Publications of Didier Marlier
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See detailParticularités de l’interprétation de l’hémogramme et des examens biochimiques sanguins chez le lapin (Oryctolagus cuniculus)
Thilliez, Natacha ULiege; Marlier, Didier ULiege

Scientific conference (2020, March 06)

Cette conférence présente les particularités de l’interprétation de l’hémogramme et des examens biochimiques sanguins chez le lapin européen (Oryctolagus cuniculus)

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See detailColumbid alphaherpesvirus-1 (Pigeon herpesvirus 1)
Marlier, Didier ULiege

in Swayne, David (Ed.) Diseases of Poultry 14th edition (2020)

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See detailBacterial osteomyelitis in a green iguana (IGUANA IGUANA)
Gandar, Frederic ULiege; Paillusseau, Clément; Deflers, Hélène ULiege et al

in Journal of Exotic Pet Medicine (2019), 28

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See detailSUCCESSFUL MEDICAL MANAGEMENT OF A BEZOAR IN A PERUVIAN GUINEA PIG (CAVIA PORCELLUS)
Deflers, Hélène ULiege; Gandar, Frédéric ULiege; Etienne, Anne-Laure ULiege et al

in Journal of Exotic Pet Medicine (2019), 29

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See detailIn vitro and in vivo assessment of eprociclovir as antiviral treatment against testudinid herpesvirus 3 in Hermann's tortoise (Testudo hermanni)
Gandar, F.; Marlier, Didier ULiege; Vanderplasschen, Alain ULiege

in Research in Veterinary Science (2019), 124

Tortoises belonging to the Testudinidae family are infected by Testudinid herpesviruses. Testudinid herpesvirus 3 (TeHV-3) is considered the most pathogenic and affects several tortoise species ... [more ▼]

Tortoises belonging to the Testudinidae family are infected by Testudinid herpesviruses. Testudinid herpesvirus 3 (TeHV-3) is considered the most pathogenic and affects several tortoise species, particularly those from the Testudo genus. As most species of this genus are endangered contribute to ecological concerns over this virus. Here, we aimed to explore the rational development of an antiviral treatment against TeHV-3 using Hermann's tortoise (Testudo hermanni) as a host model. Ten antiviral compounds were tested in cell culture for their toxicity and their activity against TeHV-3. Eight compounds exhibited different levels of activity against TeHV-3 with either no or only minor cytotoxic effects on cells. Next, eprociclovir (EPV, ciprovir) was selected for further investigations in vivo. Its pharmacokinetic properties were investigated after a single sub-cutaneous administration at 5 or 10 mg/kg. Plasma concentrations remained above half maximal effective concentration (EC 50 ) for 2.2 and 4.4 h after administration at 5 and 10 mg/kg, respectively. Finally, EPV toxicity was investigated after administration at the dose of 10 mg/kg, BID for seven consecutive days. As early as one day after initiation of the treatment up to its end, EPV plasma concentration remained under the EC 50. Apathy and anorexia developed after 7 days. Biochemical and anatomopathological examinations revealed nephrotoxic effects of EPV. Altogether, these data suggest that EPV is not a suitable molecule for the treatment of TeHV-3. Further studies are required to determine whether the other molecules identified here for their anti-TeHV-3 activity represent potential candidates for the development of efficacious treatments. © 2019 Elsevier Ltd [less ▲]

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See detailInfluence of a single dose of buprenorphine on rabbit (Oryctolagus cuniculus) gastrointestinal motility
Deflers, Hélène ULiege; Gandar, Frederic ULiege; Bolen, Géraldine ULiege et al

in Veterinary Anaesthesia and Analgesia (2018)

OBJECTIVE: To establish a noninvasive imaging protocol for rabbit gastrointestinal transit evaluation. To assess the effect of a single injection of buprenorphine on the digestive transit of rabbits via ... [more ▼]

OBJECTIVE: To establish a noninvasive imaging protocol for rabbit gastrointestinal transit evaluation. To assess the effect of a single injection of buprenorphine on the digestive transit of rabbits via this new technique. STUDY DESIGN: Prospective, parallel study. ANIMALS: Fifteen specific pathogen-free male New Zealand White rabbits weighing 2.68 ± 0.28 kg. METHODS: A 10 mL kg-1 barium meal was administered and the rabbits were subjected to serial radiographic and ultrasound examinations without treatment and 1 week later following a single intramuscular dose of 100 μg kg-1 of buprenorphine. Radiographic data from the stomach and caecum were collected and assigned a retention score ranging from 0 (no barium) to 3 (large amount of barium). The resulting scores and pyloric and duodenal contraction counts were analysed using a mixed linear model and are expressed as least square mean (lsm) ± standard error. Transit was estimated based on the apparition time of faeces in the pelvic area and analysed using a Wilcoxon test. A p < 0.05 was considered significant. RESULTS: Buprenorphine treatment induced a higher lsm number of pyloric (1.73 ± 0.19 versus 0.78 ± 0.19, p < 0.01) and lsm duodenal contractions (17.35 ± 1.04 versus 13.44 ± 1.04, p < 0.01). Buprenorphine administration decreased the lsm barium retention score in the stomach (2.44 ± 0.05 versus 2.64 ± 0.05, p < 0.01), but had no effect on the lsm barium retention score in the caecum. The time to apparition of faeces in the pelvic area was not influenced by buprenorphine administration (p = 0.66). CONCLUSIONS AND CLINICAL RELEVANCE: A single high dose of buprenorphine appears to have no adverse effect on gastrointestinal motility in healthy rabbits. [less ▲]

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See detailBenign ovarian teratoma in a central bearded dragon (Pogona vitticeps)
Gandar, Frederic ULiege; Paillusseau, Clément; Deflers, Hélène ULiege et al

Conference (2018, March 23)

A six-year-old female central bearded dragon (Pogona vitticeps) was examined for a six-month history of increasing coelomic distention, dyspnea, dysorexia, and tenesmus. Complementary examinations ... [more ▼]

A six-year-old female central bearded dragon (Pogona vitticeps) was examined for a six-month history of increasing coelomic distention, dyspnea, dysorexia, and tenesmus. Complementary examinations revealed hyperuricemia, hyperphosphatemia, increased creatine kinase, and a heterogeneous polycystic mass with peripheral coelomic effusion. An exploratory coeliotomy revealed a mass at the anatomical location of the right ovary and follicular stasis of the left ovary; bilateral ovariectomy was performed. After anatomopathological and bacterial examination, the bearded dragon was diagnosed with benign ovarian teratoma and secondary bacterial infection. Either no evidence of reoccurrence was visible by radiography or ultrasonography after 10 weeks and the owner was advised to regularly return for follow-up ultrasound examinations. To the best authors’ knowledge, this is the first case report of a benign ovarian teratoma in this species and ovarian teratoma should be included in the differential diagnosis of coelomic distention in saurian females [less ▲]

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See detailInfectious diseases diagnosis and vaccination in pet rabbits : an everyday challenge
Marlier, Didier ULiege

in Marlier, Didier (Ed.) Proceedings of the Third International Conference on Avian heRpetological and Exotic mammal medicine (2017, March 25)

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See detailEpizootische Enteropathie bij konijnen : Verleden, heden en toekomst
Marlier, Didier ULiege

Scientific conference (2016, April 13)

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See detail16e Franse studiedagen betreffende konijnenonderzoek
Marlier, Didier ULiege

Scientific conference (2016, April 13)

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See detailAntibiotherapy in pet rodents and rabbits: a preliminary analysis of practitioners’ habits
Marlier, Didier ULiege

in Proceedings of the European College of Zoological Medicine Symposium (2016, April)

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See detailLes kystes ovariens chez le cobaye
Gillet, Aline; Deflers, Hélène ULiege; Marlier, Didier ULiege

in Revue de Médecine Vétérinaire (2016), 167(3-4), 51-58

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See detailL’Entéropathie Epizootique des lapins : passé, présent et futur
Marlier, Didier ULiege

in ITAVI (Ed.) Proceedings des 16èmes journées de la recherche cunicole (2015, November 24)

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See detailThe genome of a tortoise herpesvirus (testudinid herpesvirus 3) has a novel structure and contains a large region that is not required for replication in vitro or virulence in vivo.
Gandar, Frederic ULiege; Wilkie, Gavin S.; Gatherer, Derek et al

in Journal of Virology (2015), 89(22), 11438-11456

Testudinid herpesvirus 3 (TeHV-3) is the causative agent of a lethal disease affecting several tortoise species. The threat that this virus poses to endangered animals is focusing efforts on ... [more ▼]

Testudinid herpesvirus 3 (TeHV-3) is the causative agent of a lethal disease affecting several tortoise species. The threat that this virus poses to endangered animals is focusing efforts on characterizing its properties, in order to enable the development of prophylactic methods. We have sequenced the genomes of the two most studied TeHV-3 strains (1976 and 4295). TeHV-3 strain 1976 has a novel genome structure and is most closely related to a turtle herpesvirus, thus supporting its classification into genus Scutavirus, subfamily Alphaherpesvirinae, family Herpesviridae. The sequence of strain 1976 also revealed viral counterparts of cellular interleukin-10 and semaphorin, which have not been described previously in members of subfamily Alphaherpesvirinae. TeHV-3 strain 4295 is a mixture of three forms (m1, m2, and M), in which, in comparison to strain 1976, the genomes exhibit large, partially overlapping deletions of 12.5 to 22.4 kb. Viral subclones representing these forms were isolated by limiting dilution assays, and each replicated in cell culture comparably to strain 1976. With the goal of testing the potential of the three forms as attenuated vaccine candidates, strain 4295 was inoculated intranasally into Hermann's tortoises (Testudo hermanni). All inoculated subjects died, and PCR analyses demonstrated the ability of the m2 and M forms to spread and invade the brain. In contrast, the m1 form was detected in none of the organs tested, suggesting its potential as the basis of an attenuated vaccine candidate. Our findings represent a major step toward characterizing TeHV-3 and developing prophylactic methods against it. [less ▲]

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See detailImproving adjuvant systems for polyclonal egg yolk antibody (IgY) production in laying hens in terms of productivity and animal welfare
Marcq, Christopher ULiege; Marlier, Didier ULiege; Beckers, Yves ULiege

in Veterinary Immunology and Immunopathology (2015), 165(1-2), 54-63

The antibody production in the egg yolks of immunized laying hens is seen as a way of improving animal welfare compared with conventional production by mammals. Immunoglobulin Y (IgY) technology, however ... [more ▼]

The antibody production in the egg yolks of immunized laying hens is seen as a way of improving animal welfare compared with conventional production by mammals. Immunoglobulin Y (IgY) technology, however, has still to address welfare issues linked to the widespread use of an adjuvant in vaccines. Currently, Freund's adjuvants, complete (FCA) or incomplete (FIA), remain the standard. This study sought to evaluate various approaches used to enhance egg yolk antibody production in terms of both productivity and avian welfare. The outer membrane protein (OMP) of Salmonella Typhimurium was used as the prototype antigen. At 20 weeks of age, 56 ISA Brown hens, with specific-Salmonella-free status, were divided into seven groups (n = 8) and received an initial intramuscular immunization. Hens in the two negative control groups received phosphate buffered saline (PBS) or FIA alone. Hens in the other groups received 80 μg of Salmonella OMP emulsified with one of the following adjuvants: 200 μl of FIA alone (T1); 200 μl of FIA supplemented with 8 μg of C-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) (T2); and 280 μl of Montanide ISA 70 VG (T4). Birds in the T3 group received the antigen in emulsion with FIA and were given the tested immunostimulatory component (l-carnitine) via their feed (100 mg/kg). A positive control group (PC) received FCA for the first and final immunizations and FIA for the other boosters. Immunization was repeated after 20, 46, 82 and 221 days. Eggs were collected regularly until 242 days after the first immunization and the anti-Salmonella Typhimurium activities in the yolk were determined by ELISA. After 242 days, the birds were euthanized and the injection sites were evaluated for gross and microscopic lesions. Among the tested immunostimulatory approaches, supplementation of FIA with CpG-ODN led to a significant and long-lasting enhancement of the specific antibody response. This treatment was even higher than the positive benchmark using FCA in the first immunization. The study results showed that a clinical examination of injection sites is insufficient for drawing conclusions about the local tolerance of vaccines. Tissue damage was noticeable in all treatment groups. The birds receiving the Montanide adjuvant, however, had fewer and less severe lesions. Given these limited side-effects, Montanide ISA 70 VG could provide the depot effect needed to ensure the immunomodulatory efficiency of CpG-ODN. The association of these two adjuvants could prove a promising alternative to Freund's adjuvants (FA). [less ▲]

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See detailLes pathologies liées aux conditions d’entretien chez le lapin de compagnie
Marlier, Didier ULiege

Scientific conference (2015, March 06)

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See detailMaladies digestives du lapin
Marlier, Didier ULiege

in Gidenne, Thierry (Ed.) Le lapin. De la biologie à l'élevage. (2015)

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