Publications of Marie-Laure Fauconnier
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See detailChemical composition and anti-inflammatory activity of Apium graveolens var. dulce essential oils from Senegal
Thiam, Abdoulaye; Gueye, Momar Talla; Sanghare, Cheickna Hamala et al

in American Journal of Food Science and Technology (2020), 20(8(6)), 226-232

Apium graveolens var. dulce commonly known as celery, belongs to the Apiaceae family. It is used especially as a vegetable and medicinal plant. In Senegal, A. graveolens is used as a food condiment. The ... [more ▼]

Apium graveolens var. dulce commonly known as celery, belongs to the Apiaceae family. It is used especially as a vegetable and medicinal plant. In Senegal, A. graveolens is used as a food condiment. The aim of this work is to study the chemical composition of A. graveolens stems (S), leaves (L) essential oils and anti-inflammatory activity. GC/FID and GC/MS analyzes carried out on essential oils obtained by steam distillation showed that oils from both stems and leaves were characterized by the same major constituents α-pinene, the prominent compound of oils represented 69.3 and 68.4% for stems (S) and leaves (L), respectively. It is followed by limonene (9.5 and 9.8%), α-phellandrene (5.5 and 5.9%) and β-pinene (4.8 and 4.3%). These compounds represented 89.1% (S) and 88.4% (L) of the total essential oils content. Anti-inflammatory activity was measured by the inhibition of 5-lipoxygenase (5-LOX) by A. graveolens essential oil anti-inflammatory assays revealed an IC50 of 29.5±2.0 µg/mL for A. graveolens oils and 23.7±0.5 µg/mL for quercetine used as a reference. This study showed that essential oils of A. graveolens was an important source of α-pinene who is probably responsible of its anti-inflammatory properties. [less ▲]

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See detailOptimization of gallic acid encapsulation in calcium alginate microbeads using Box-Behnken Experimental Design
Lakrat, Mohammed; Berraaouan, Doha; Fauconnier, Marie-Laure ULiege et al

in Polymer bulletin (2020)

The aim of this study was the optimization of the gallic acid (GA) encapsulation efficiency within calcium alginate microparticles by the ionotropic gelation technique, using Box-Behnken design for the ... [more ▼]

The aim of this study was the optimization of the gallic acid (GA) encapsulation efficiency within calcium alginate microparticles by the ionotropic gelation technique, using Box-Behnken design for the surface methodology response. For this purpose, three independent variables were selected: sodium alginate concentration (X1), calcium chloride concentration (X2), and gallic acid concentrations (X3). The influence of each variable on the encapsulation efficiency was evaluated. The optimum conditions to reach maximum encapsulation efficiency were found to be: X1 = 30 g/l (3%, w/v), X2 = 21.63 g/l (2.163%, w/v) and X3 = 15 g/l (1.5%, w/v), respectively. The encapsulation efficiency was determined to be 42.8%. The obtained microbeads were further examined using differential scanning calorimetry (DSC) and Fourier transform infrared (ATR-FTIR), and the inclusion of gallic acid was confirmed. The gallic acid concentration (X3) is the statistically significant factor in the optimization process. In addition, no autoxidation of the gallic acid compound was observed in the formulated calcium alginate microbeads. Scanning electron microscope (SEM) analysis showed that the shape of the particle was spherical for all formulations and their surface is wrinkled. The release study of the gallic acid carried out in an aqueous medium at pH value 6.8, showed that the GA release pattern was fast for all systems studied (85% at 20 min), and the profile of the release was influenced by the size of the calcium alginate microbeads. The obtained results reveal that the calcium alginate microbeads prepared through the ionotropic gelation technique possess great prominent for gallic acid encapsulation as well as its liberation. [less ▲]

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See detailIdentification of a proanthocyanidin from Litchi chinensis Sonn. root with anti-tyrosinase and antioxidant activity.
Saive, Matthew ULiege; Genva, Manon ULiege; Istasse, Thibaut ULiege et al

in Biomolecules (2020)

This work follows an ethnobotanical study that took place in the island of Mayotte (France), which pointed out the potential properties of Litchi chinensis Sonn. roots when used to enhance skin health and ... [more ▼]

This work follows an ethnobotanical study that took place in the island of Mayotte (France), which pointed out the potential properties of Litchi chinensis Sonn. roots when used to enhance skin health and appearance. Through in vitro testing of a crude methanolic extract, high anti-tyrosinase (skin whitening e ect) and antioxidant activities (skin soothing e ect) could be measured. HPLC successive bio-guided fractionation steps allowed the purification of one of the compounds responsible for the biological activities. The isolated compound was characterized by UV, IR, MS and 2D-NMR, revealing, for the first time in Litchi chinensis Sonn. roots, an A-type proanthocyanidin and thus revealing a consensus among the traditional use shown by the ethnobotanical study, in vitro biological activities and chemical characterization. [less ▲]

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See detailSimultaneous determination of 14 bioactive citrus flavonoids using thin-layer chromatography combined with surface enhanced Raman spectroscopy
Li, Yuzhi ULiege; Zhao, Chengying; Lu, Chang et al

in Food Chemistry (2020)

Citrus flavonoids consist of diverse analogs and possess various health-promoting effects dramatically depending on their chemical structures. Since different flavonoids usually co-exist in real samples ... [more ▼]

Citrus flavonoids consist of diverse analogs and possess various health-promoting effects dramatically depending on their chemical structures. Since different flavonoids usually co-exist in real samples, it’s necessary to develop rapid and efficient methods for simultaneous determination of multiple flavonoids. Herein, thin layer chromatography combined with surface enhanced Raman spectroscopy (TLC-SERS) was established to simultaneously separate and detect 14 main citrus flavonoids for the first time. These target compounds could be characterized and discriminated when paired with SERS at 6-500 times greater the sensitivity than TLC alone. TLC-SERS exhibited high recovery rates (91.5-121.7%) with relative standard deviation (RSD) lower than 20.8%. Moreover, the established TLC-SERS method was successfully used to simultaneously detect multiple flavonoids in real samples, which exhibited comparable accuracy to high performance liquid chromatography (HPLC) with shorter analytical time (10 vs 45 min). All the results demonstrated that this could be a promising method for simultaneous, rapid, sensitive and accurate detection of flavonoids. [less ▲]

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See detailPhytotoxicity of essential oils: opportunities and constraints for the development of biopesticides. A review
Werrie, Pierre-Yves ULiege; Durenne, Bastien; Delaplace, Pierre ULiege et al

in Foods (2020)

Abstract: The extensive use of chemical pesticides leads to risks for both the environment and human health due to the toxicity and poor biodegradability that they may present. Farmers therefore need ... [more ▼]

Abstract: The extensive use of chemical pesticides leads to risks for both the environment and human health due to the toxicity and poor biodegradability that they may present. Farmers therefore need alternative agricultural practices including the use of natural molecules to achieve more sustainable production methods to meet consumer and societal expectations. Numerous studies have reported the potential of essential oils as biopesticides for integrated weed or pest management. However, their phytotoxic properties have long been a major drawback for their potential applicability (apart from herbicidal application). Therefore, deciphering the mode of action of essential oils exogenously applied in regards to their potential phytotoxicity will help in the development of biopesticides for sustainable agriculture. Nowadays, plant physiologists are attempting to understand the mechanisms underlying their phytotoxicity at both cellular and molecular levels using transcriptomic and metabolomic tools. This review systematically discusses the functional and cellular impacts of essential oils applied in the agronomic context. Putative molecular targets and resulting physiological disturbances are described. New opportunities regarding the development of biopesticides are discussed including biostimulation and defense elicitation or priming properties of essential oils. [less ▲]

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See detailSimple liquid chromatography-electrospray ionization ion trap mass spectrometry method for the quantification of galacto-oxylipin arabidopsides in plant samples
Genva, Manon ULiege; Anderson, Mats; Fauconnier, Marie-Laure ULiege

in Scientific Reports (2020)

A simple and sensitive method to quantify fve diferent arabidopsides by HPLC—ion trap mass spectrometry in complex plant samples was developed and validated. Arabidopsides are oxidized galactolipids frst ... [more ▼]

A simple and sensitive method to quantify fve diferent arabidopsides by HPLC—ion trap mass spectrometry in complex plant samples was developed and validated. Arabidopsides are oxidized galactolipids frst described in Arabidopsis thaliana but also produced by other plant species under stress conditions. External calibration was performed using arabidopsides purifed from freezethawed Arabidopsis leaves. Lipids were extracted and pre-purifed on an SPE silica column before HPLC–MS analysis.Arabidopsides were separated on a C18 column using a gradient of mQ water and acetonitrile:mQ water (85:15) supplemented with formic acid (0.2%) and ammonium formate (12 mM).The method was validated according to European commission decision 2002/657/CE. LOD, LOQ, linearity, intra-day and inter-day precision and accuracy, selectivity, matrix efects and recoveries were determined for the fve metabolites.The established method is highly selective in a complex plant matrix. LOD and LOQ were, respectively, in the range 0.098–0.78 and 0.64–1.56 µM, allowing the arabidopside quantifcation from 25.6–62.4 nmol/g fresh weight. Calibration curve correlation coefcients were higher than 0.997. Matrix efects ranged from -2.09% to 6.10% and recoveries between 70.7% and 109%.The method was successfully applied to complex plant matrixes: Arabidopsis thaliana and Nasturtium officinale. [less ▲]

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See detailAbout lipid metabolism in Hermetia illucens (L. 1758). On the origin of fatty acids in prepupae.
Hoc, Bertrand ULiege; Genva, Manon ULiege; Fauconnier, Marie-Laure ULiege et al

in Scientific Reports (2020)

Although increasingly targeted in animal nutrition, black soldier fly larvae or prepupae (BSF, Hermetia illucens L. 1758) require the characterization and modulation of their fatty acid profile to become ... [more ▼]

Although increasingly targeted in animal nutrition, black soldier fly larvae or prepupae (BSF, Hermetia illucens L. 1758) require the characterization and modulation of their fatty acid profile to become fully integrated within the feed sector. This improvement will only be possible by the understanding of underlaying biochemical pathways of fatty acid synthesis in BSF. In this study, we hypothesized a labelling of de novo synthesized fatty acids in BSF by the incorporation of deuterated water ( D2O) in their feed. Three batches of fifty larvae were reared on two diets with different polyunsaturated fatty acid profiles moistened with 40% of H2O or D2O: chicken feed or 40% of chicken feed and 60% of flax cake. Although the occurrence of D2O in insect feed increased the larval development time and decreased prepupal weight, it was possible to track the biosynthesis of fatty acids through deuterium labelling. Some fatty acids (decanoic, lauric or myristic acid) were exclusively present in their deuterated form while others (palmitic, palmitoleic or oleic acid) were found in two forms (deuterated or not) indicating that BSF can partially produce these fatty acids via biosynthesis pathways and not only by bioaccumulation from the diet. These results suggest the importance of carbohydrates as a source of acetyl-CoA in the constitution of the BSF fatty acid profile but also the potential importance of specific enzymes (e.g. thioesterase II or Δ12 fat2 desaturase) in BSF fatty acid metabolism. Finally, nearly no deuterated polyunsaturated fatty acids were found in BSF fed with deuterium confirming that BSF is not able to produce these types of fatty acids. Despite the high levels of linolenic acid in flax-enriched diets, BSF will simply bioaccumulate around 13% of this fatty acid and will metabolize approximately two-thirds of it into saturated fatty acids as lauric or myristic acid. [less ▲]

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See detailTemporal evolution of PAHs bioaccessibility in an aged-contaminated soil during the growth of two Fabaceae.
Davin, Marie ULiege; Renard, Elisa; Lefébure, Kévin ULiege et al

in International Journal of Environmental Research and Public Health (2020)

Polycyclic aromatic hydrocarbons (PAHs) are health-concerning organic compounds that accumulate in the environment. Bioremediation and phytoremediation are studied to develop eco-friendly remediation ... [more ▼]

Polycyclic aromatic hydrocarbons (PAHs) are health-concerning organic compounds that accumulate in the environment. Bioremediation and phytoremediation are studied to develop eco-friendly remediation techniques. In this study, the e ects of two plants (Medicago sativa L. and Trifolium pratense L.) on the PAHs’ bioaccessibility in an aged-contaminated soil throughout a long-term rhizoremediation trial was investigated. A bioaccessibility measurement protocol, using Tenax® beads, was adapted to the studied soil. The aged-contaminated soil was cultured with each plant type and compared to unplanted soil. The bioaccessible and residual PAH contents were quantified after 3, 6 and 12 months. The PAHs’ desorption kinetics were established for 15 PAHs and described by a site distribution model. A common Tenax® extraction time (24 h) was established as a comparison basis for PAHs bioaccessibility. The rhizoremediation results show that M. sativa developed better than T. pratense on the contaminated soil. When plants were absent (control) or small (T. pratense), the global PAHs’ residual contents dissipated from the rhizosphere to 8% and 10% of the total initial content, respectively. However, in the presence of M. sativa, dissipation after 12 months was only 50% of the total initial content. Finally, the PAHs’ bioaccessible content increased more significantly in the absence of plants. This one-year trial brought no evidence that the presence of M. sativa or T. pratense on this tested aged-contaminated soil was beneficial in the PAH remediation process, compared to unplanted soil. [less ▲]

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See detailInfluence of climate variation on phenolic composition and antioxidant capacity of Medicago minima populations
Kabtni, Souhir; Sdouga, Dorra; Bettaieb Rebay, Ines et al

in Scientific Reports (2020)

Medicago minima is a pasture legume that grows almost all over the world. In Tunisia, it occupies various climatic environments and is considered the most abundant annual Medicago plant. However, this ... [more ▼]

Medicago minima is a pasture legume that grows almost all over the world. In Tunisia, it occupies various climatic environments and is considered the most abundant annual Medicago plant. However, this species is unconsumed and unused by humans. This study aimed to explore the phytochemical characteristics of Medicago minima selected from diferent provenances in Tunisia and subsequently investigate the infuence of environmental factors on their phenolic composition and antioxidant activity. Therefore, a calorimetric method and DPPH tests provided the total phenolic and total favonoid contents and antioxidant potential in roots, stems, leaves and seeds. High performance liquid chromatography (HPLC) identifed and quantifed four phenolic acids and three favonoids in the studied organs. Roots and leaves showed the greatest phenolic compound content and had high antioxidant activity. Rutin and syringic acid (leaves) represent a characteristic for this species. For each organ, principal component analysis of phenolic profles showed that the root’s phenolic composition could be an indication of the plant adaptation to even small changes in its environments. Plants originating from a cold climate, higher altitude or semi-arid environment had the highest phenolic compound contents in their organs. Our fndings provide useful information for the exploitation of the phenolic compounds in these weeds for the development of environmental sustainability. [less ▲]

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See detailComposition, Seasonal Variation and Biological Activities of Lantana camara essential oils from Côte d’Ivoire.
Nea, Fatimata ULiege; Kambiré, Didjour Albert; Genva, Manon ULiege et al

in Molecules (2020)

This work aims to study the variations in the composition of Lantana camara leaf, flower and stem essential oils over two years. L. camara organs were harvested in Bregbo (East Côte d'Ivoire) each month ... [more ▼]

This work aims to study the variations in the composition of Lantana camara leaf, flower and stem essential oils over two years. L. camara organs were harvested in Bregbo (East Côte d'Ivoire) each month from June 2015 to June 2017. The essential oils were obtained by hydrodistillation and characterized by GC-MS and 13C NMR. Eighty-four compounds accounting for 84.4 – 99.1% of the essential oils have been identified. The essential oils hydrodistillated from L. camara are dominated by sesquiterpenes such as (E)-β-caryophyllene and α-humulene, which were found in all samples. Some monoterpenes such as thymol, sabinene and α-pinene were also present. Statistical analysis (principal component analysis and clustering) revealed a high variability in essential oil composition between the different organs and also within the studied periods as the thymol proportion was higher during flowering and fruiting months. In addition, the stem, flower, and fruit essential oils were more concentrated in thymol than the leaf essential oils. The proportions of (E)-β-caryophyllene and α-humulene were strictly inverted with the thymol proportion throughout the harvest period or vegetative cycle. The antioxidant, anti-inflammatory and insecticidal activities of leaves and flowers essential oils were also studied. Results showed that L. camara leaf and flower essential oils displayed high antioxidant, anti-inflammatory and insecticidal activities. [less ▲]

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See detailLinolenic fatty acid hydroperoxide acts as biocide on plant pathogenic bacteria: biophysical investigation of the mode of action
Deboever, Estelle ULiege; Lins, Laurence ULiege; Ongena, Marc ULiege et al

in Bioorganic Chemistry (2020)

Fatty acid hydroperoxides (HPO) are free phyto-oxylipins known for their crucial role as signalling molecules during plant defense mechanisms. They were also demonstrated to have direct biocidal ... [more ▼]

Fatty acid hydroperoxides (HPO) are free phyto-oxylipins known for their crucial role as signalling molecules during plant defense mechanisms. They were also demonstrated to have direct biocidal activities against plant pathogens including gram negative bacteria. In the present work, the biocidal effect of one linolenic fatty acid hydroperoxide, namely 13-HPOT has been investigated on three plant pathogen gram negative bacteria: Pectobacterium carotovorum, Pseudomonas syringae and Xanthomonas translucens. We showed that 13-HPOT has a strong dose response effect on those phytopathogens. In a second part, the molecular mechanism behind the antibacterial effect of 13-HPOT was investigated at a molecular level using an integrative biophysical approach combining in vitro and in silico methods. Since other antimicrobial amphiphilic molecules have been shown to target the lipid membrane of the organisms they act on, we focused our study on the interaction of 13-HPOT with biomimetic membranes. In a first step, we hypothesized that the inner membrane of the bacteria was the main site of action of 13-HPOT and hence we used lipids representative of this membrane to form our models. Our results indicated that 13-HPOT can interact with the lipid representative of the inner bacterial plasma membrane. A strong membrane insertion is suggested but no major permeabilization of the membrane is observed. Phosphatidylethanolamine (PE) and cardiolipin (CL), present in the bacterial plasma membrane, appear to play important roles in this interaction. We suggest that the mode of action of 13-HPOT should involve either subtle changes in membrane properties, such as its lateral organization and distribution, and/or interactions with membrane proteins. [less ▲]

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See detailSensitive and simultaneous SERS detection of different pathogens based on aptamer and Raman reporter co-mediated gold tags
Li, Yuzhi ULiege; Lu, Chang; Zhou, Shuaishuai et al

in Sensors and Actuators. B, Chemical (2020)

A biosensor based on novel SERS tags, consisting of gold nanorods (GNRs) complexed with oligonucleotide aptamers and the Raman reporters, was developed for the sensitive and simultaneous detection of ... [more ▼]

A biosensor based on novel SERS tags, consisting of gold nanorods (GNRs) complexed with oligonucleotide aptamers and the Raman reporters, was developed for the sensitive and simultaneous detection of different food pathogens. The aptamers not only act as bio-recognition molecules, but along with the Raman reporters, induce the GNRs to grow to specific shapes, which in turn enhance the Raman signal and facilitate sensitive detection. Signal interference during the simultaneous detection of pathogens is avoided, due to the stable anchored aptamers and embedded Raman reporters. We combined the novel SERS tags with antibody-modified magnetic nanoparticles to create a biosensor capable of simultaneous detection of Escherichia coli O157:H7 and Salmonella typhimurium with good linear response (101 to 106 cfu/mL), high detection sensitivity (<8 cfu/mL) and recovery rate (95.26–107.88%) in spiked food samples. This strategy achieves the goal of sensitive and simultaneous quantitative detection of pathogens. [less ▲]

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See detailVarietal susceptibility of maize to larger grain borer, Prostephanus truncatus (Horn) (Coleoptera; Bostrichidae), based on grain physicochemical parameters
Ngom, Déthié; Fauconnier, Marie-Laure ULiege; Malumba Kamba, Paul ULiege et al

in PLoS ONE (2020)

Maize (Zea mays L) is one of main nutrients sources for humans and animals worldwide. In Africa, storage of maize ensures food resources availability throughout the year. However, it often suffers losses ... [more ▼]

Maize (Zea mays L) is one of main nutrients sources for humans and animals worldwide. In Africa, storage of maize ensures food resources availability throughout the year. However, it often suffers losses exceeding 20% due to insects such as the larger grain borer, Prostephanus truncatus (Horn) (Coleoptera; Bostrichidae), major pest of stored maize in the tropical countries. This study aims to select resistant varieties to reduce maize storage losses and explain the physicochemical parameters role in grains susceptibility. In the first study, maize grains were artificially infested under no-choice method with insects. Susceptibility parameters such as weight loss, grain damage, number of emerged insects, median development time and susceptibility index varied significantly through maize varieties. Dobie susceptibility index (SI) was assessed as a major indicator of resistance. The most resistant varieties were Early-Thaï, DMR-ES and Tzee-Yellow. Conversely, Synth-9243, Obatampa and Synth-C varieties were susceptible. SWAN, Across-Pool and Tzee-White were classified as moderately resistant varieties. The insect reproductive potential was significantly different in the nine maize varieties and Early-Thaï, DMR-ES and Tzee-Yellow varieties were the least favourable host. To assess the relationship between grains physicochemical characteristics and varietal susceptibility, moisture, total phenolics, palmitic acid, proteins, amylose, density and grain hardness were evaluated according to standardized methods. Palmitic acid, SI, insects emerged and grain damage were significantly and positively correlated with each other, and negatively correlated with grains hardness, phenolics and amylose contents. Maize susceptibility index was significantly and negatively correlated to amylose, and phenolics contents and positively correlated to palmitic acid content. This study identified three resistant maize varieties to P. tuncatus and revealed that the major factors involved in this resistance were hardness, phenolic and amylose contents of grains. [less ▲]

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See detailSeasonal effect on the chemical composition, insecticidal properties and other biological activities of Zanthoxylum leprieurii Guill. & Perr. essential oils
Tanoh, Amenan Evelyne ULiege; Blanchard Boué, Guy; Nea, Fatimata ULiege et al

in Foods (2020)

This study focused, for the first time, on the evaluation of the seasonal effect on the chemical composition and biological activities of essential oils hydrodistillated from leaves, trunk bark and fruits ... [more ▼]

This study focused, for the first time, on the evaluation of the seasonal effect on the chemical composition and biological activities of essential oils hydrodistillated from leaves, trunk bark and fruits of Zanthoxylum leprieurii (Z. leprieurii), a traditional medicinal wild plant growing in Côte d’Ivoire. The essential oils were obtained by hydrodistillation from fresh organs of Z. leprieurii growing on the same site over several months using a Clevenger‐type apparatus and analyzed by gas chromatography‐mass spectrometry (GC/MS). Leaf essential oils were dominated by tridecan‐ 2‐one (9.00 ± 0.02–36.80 ± 0.06%), (E)‐β‐ocimene (1.30 ± 0.50–23.57 ± 0.47%), β‐caryophyllene (7.00 ± 1.02–19.85 ± 0.48%), dendrolasin (1.79 ± 0.08–16.40 ± 0.85%) and undecan‐2‐one (1.20 ± 0.03–8.51 ± 0.35%). Fruit essential oils were rich in β‐myrcene (16.40 ± 0.91–48.27 ± 0.26%), citronellol (1.90 ± 0.02–28.24 ± 0.10%) and geranial (5.30 ± 0.53–12.50 ± 0.47%). Tridecan‐2‐one (45.26 ± 0.96–78.80 ± 0.55%), β‐caryophyllene (1.80 ± 0.23–13.20 ± 0.33%), 𝛼‐humulene (4.30 ±1.09–12.73 ± 1.41%) and tridecan‐2‐ol (2.23 ± 0.17–10.10 ± 0.61%) were identified as major components of trunk bark oils. Statistical analyses of essential oil compositions showed that the variability mainly comes from the organs. Indeed, principal component analysis (PCA) and hierarchical cluster analysis (HCA) allowed us to cluster the samples into three groups, each one consisting of one different Z. leprieurii organ, showing that essential oils hydrodistillated from the different organs do not display the same chemical composition. However, significant differences in essential oil compositions for the same organ were highlighted during the studied period, showing the impact of the seasonal effect on essential oil compositions. Biological activities of the produced essential oils were also investigated. Essential oils exhibited high insecticidal activities against Sitophilus granarius, as well as antioxidant, anti‐inflammatory and moderate anti‐plasmodial properties. [less ▲]

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See detailAptamer-based biosensor for detection of mycotoxins
Guo, Xiaodong; Wen, Fang; Zheng, Nan et al

in Frontiers in Chemistry (2020)

Mycotoxins are a large types of secondary metabolites appeared by fungi, they pose a great hazard and toxic reactions to human and animals. A majority of countries and regulators, such as European Union ... [more ▼]

Mycotoxins are a large types of secondary metabolites appeared by fungi, they pose a great hazard and toxic reactions to human and animals. A majority of countries and regulators, such as European Union, have established series of requirements and set the maximum tolerated levels. The development of high sensitive and specific analytical platform for mycotoxins is much in demand to address new challenges for food safety in worldwide. Due to the superiority of simple, rapid, and low-cost characteristics, aptamer-based biosensors are successfully developed for the detection of various mycotoxins with high sensitivity and selectivity compared with traditional instrumental methods and immunological approaches. In this article, we discuss and analyze the development of aptasensors for mycotoxins determination in food and agricultural products during the last eleven years and cover the literatures from the first report in 2008 until today. In addition, challenges and future trends for the selection of aptamers towards various mycotoxins and aptasensors for multi-mycotoxins analysis are summarized. Given the promising development and potential application of aptasensors, the future researches will witness the great practicability of aptamer-based biosensor for food safety field. [less ▲]

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See detailAptasensors Technologies for Aflatoxins B1 Application
Guo, Xiaodong; Fauconnier, Marie-Laure ULiege; Wang, Jiaqi

in Current Trends in Veterinary and Dairy Research (2020)

Mycotoxins, highly toxic metabolites occurred by various molds, can contaminate foods and agricultural products, which mainly includes maize, cereals, nuts, meat, milk, wine, and fruits. Aflatoxin B1 ... [more ▼]

Mycotoxins, highly toxic metabolites occurred by various molds, can contaminate foods and agricultural products, which mainly includes maize, cereals, nuts, meat, milk, wine, and fruits. Aflatoxin B1 (AFB1), one of the most important and toxic mycotoxins, has been classified as group 1 carcinogen by the International Agency for Research on Cancer (IARC) of the World Health Organization (WHO) [1]. The toxicity of AFB1 is over than KCN, arsenic and melamine by 10, 68, and 416 times, respectively. The maximum tolerated level of AFB1 was established at 2 μg kg-1 for all cereals and cereal-derived products by European Commission [2]. Traditional AFB1 detection methods are instrumental approaches (like High-Performance Liquid Chromatography (HPLC)) and antibody-based immunoassays (like Enzyme-Linked Immune Sorbent Assay (ELISA)). Aptamers, considered as “chemical antibodies”, were obtained and selected by systematic evolution of the ligand by the exponential enrichment process (SELEX) in vitro. They can specifically recognize various target with high affinity like or even superior to antibodies [3]. Aptamer-based biosensors have been widely applied for the detection of AFB1 in recent years. More importantly, in order to achieve the performance of simple and rapid detection, low cost, high sensitivity and specificity, current technologies were employed to construct aptasensors for AFB1 determination. [less ▲]

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See detailDetermination of Physico-biochemical Proprieties and Composition in Volatile Constituents by Solid Phase Micro-extraction of Honey Samples from Different Botanical and Geographical Origins in Morocco
Hafida, Hanine; Fauconnier, Marie-Laure ULiege; Kenne Kemene, Tierry ULiege et al

in Journal of Apicultural Research (2020)

There is very little information available on the physicochemical proprieties and biochemical composition of the honey commercially available which are truly endangered in Morocco. None of the studied ... [more ▼]

There is very little information available on the physicochemical proprieties and biochemical composition of the honey commercially available which are truly endangered in Morocco. None of the studied honey is available for commercial purposes, which is the main interest and novelty of this study. The aim of this work is to characterize and classify forty seven honey samples collected from different localities in Morocco and to compare them with two foreign honey samples from Ghana and France, based on their physicochemical proprieties, phenolic contents, radical scavenging activity and volatile compounds by SPME-GC/MS were used to evaluate the quality and cluster all honey samples. Variance analysis revealed highly significant differences between samples (p<0.05). Monofloral honey was characterized by the highest concentration of proline (292.77 ± 13.30). Mutifloral honey from France showed higher amounts of diastase (17.50 ± 1.80) than other compounds, while eucalyptus honey had a higher amount of HMF (105.14 ± 3.7) than the others. Multifloral honey from Ghana showed higher contents of total phenol (149.31 ± 0.41 mg GAE/100g) and flavonoids (58.28 ± 2.6mgRu/100g) than carotenoids (40.76 ± 0.7 mg Eb carotene/100g). Thyme honey showed the highest phenol content (70.97 ± 1.35 mg GAE/100g), flavonoids content (47.18 ± 2.43 mg ERu/100g) and carotenoids content (74.94 ± 3.08mEb-carotene) than other monofloral honey and glucose honey examined. The principal components analysis (PCA) was performed in order to classify honey samples and identify the most discriminant parameters. Lastly, using ANOVA and correlations for all parameters, significant differences between diverse types of honey were examined. Biochemical and SPME/GC/MS methods were used to propose a complementary approach for honey classification. [less ▲]

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See detailOli essenziali: applicazioni in agronomia
Fauconnier, Marie-Laure ULiege

Conference (2020, February 15)

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See detailMolecular biophysics as a tool to investigate the bioherbicide effect of essential oils related to their interaction with plant plasma membrane
Lins, Laurence ULiege; De Clerck, Caroline ULiege; Fauconnier, Marie-Laure ULiege et al

Conference (2020, February)

Essential oils (EOs) are used in an increasingly number of sectors like medicine, cosmetics, food industry and more recently in agronomy. In agronomy, EOs are used as bio-pesticides for their insecticidal ... [more ▼]

Essential oils (EOs) are used in an increasingly number of sectors like medicine, cosmetics, food industry and more recently in agronomy. In agronomy, EOs are used as bio-pesticides for their insecticidal, antifungal or bactericidal effects but also as bio-herbicides. Owing to the current attraction for natural products, a better understanding of their mode of biological action for new and optimal applications is of importance. It has been shown that EOs antimicrobial activity, quite well described in the literature, is at least partly due to their interaction with the plasma membrane. They notably change the lipid composition, altering fluidity, leading to various effects which can induce cell lysis, apoptosis or necrosis. Citronellol, a major compound of lemongrass EO was notably shown to have antifungal activities by changing the membrane composition and inhibiting cell growth. Cinnamaldehyde (cinnamon EO) has been reported to have a broad spectrum of antibacterial activity, notably by affecting cell morphology, membrane integrity, permeability and composition. We are currently working on the development of a bioherbicide made from Cinnamomum zeylanicum Blume (cinnamon) and Cymbogognon winterianus Jowitt (citronella) EOs. We have shown that the application of the whole EOs and their major individual compounds on the leaves and cotyledons of A. thaliana appears to be promising: when applied on cotyledons or leaves, EOs induce damages that are as important as those observed for commercial herbicides. Since EOs are small amphiphilic molecules, they can cross the mesh of cell wall and interact directly with the plant plasma membrane (PPM). Modifying the lipid organization could lead to crucial cellular effects, notably on protein function. We used a unique and original combination of in silico (molecular dynamics simulations) and in vitro (Langmuir monolayers, isothermal calorimetry, fluorescence and infrared spectroscopies) biophysical approaches, previously developed to study structure-function relationships of molecules of biological interest (pharmacological drugs , proteins, peptides, surfactants…) to investigate the interaction of EOs or their individual compounds with bio-mimetic plant plasma membranes to better understand the structure- activity relationships in the context of their bioherbicide activity. [less ▲]

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See detailOn the effect of initial drying techniques on essential oil composition, phenolic compound and antioxidant properties of anise (Pimpinella anisum L.) seeds
Bettaieb, Iness ULiege; Bourgou, Soumaya; Ben Kaab, Sofiene et al

in Journal of Food Measurement and Characterization (2020), 14(1), 220-228

The efect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms of their essential oils, phenolics and antioxidant activities. The optimum yield of essential oil was found ... [more ▼]

The efect of drying methods (sun, oven and shade drying) on aniseeds was investigated in terms of their essential oils, phenolics and antioxidant activities. The optimum yield of essential oil was found in shade drying (2.62%). Fourteen volatile compounds were determined in all samples with variation of the main component proportions depending on drying methods. Thus, trans-anethole (84.21%) and estragole (3.82%) proportions signiicantly increased in shade drying. The highest total phenol and lavonoid contents of aniseeds were recorded in shade drying (42.70 mg of GAE/g and 53.55 mg of QE/g, respectively) while the lowest contents in oven drying at 60 °C (31.15 mg of GAE/g and 46.20 mg of QE/g, respectively). In all drying methods, naringin (41.04–43.76%), chloroginic acid (23.13–27.19%) and rosmarinic acid (12.26–15.95%) were the predominant phenol compounds. Although shade drying increased the antioxidant activity, aniseed extracts exhibited higher radical scavenging (IC50 = 10.15 μg/mL), reducing power (EC50 = 187.24 μg/mL) and chelating (IC50 = 6.85 mg/mL) capacities than essential oils (IC50 = 114.87 μg/mL, EC50 = 548.05 μg/mL and IC50 = 58.65 mg/mL, respectively). In conclusion, Shade drying method was found to enhance essential oils, phenols and antioxidant activities in aniseeds. [less ▲]

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