Publications of Sébastien Massart
Bookmark and Share    
Full Text
See detailSafeguarding and using global banana diversity: a holistic approach
Van den houwe, Ines; Chase, Rachel; Sardos, Julie et al

in CABI agriculture and Bioscience (2020)

Detailed reference viewed: 24 (4 ULiège)
Full Text
See detailIlluminating an Ecological Blackbox: Using High Throughput Sequencing to Characterize the Plant Virome Across Scales
Maclot, François ULiege; Candresse, Thierry; Filloux, Denis et al

in Frontiers in Microbiology (2020), 11(578064),

Detailed reference viewed: 25 (3 ULiège)
Full Text
See detailTactical sciences for plant viruses to foster food security: the potato case
Massart, Sébastien ULiege; De Jonghe, Kris

Conference (2020, October 15)

Detailed reference viewed: 30 (1 ULiège)
Full Text
See detailCitrus Psorosis Virus: Current Insights on a Still Poorly Understood Ophiovirus
Belabess, Zineb; Sagouti, Tourya; Rhallabi, Naima et al

in Microorganisms (2020), 8

Detailed reference viewed: 23 (3 ULiège)
Full Text
See detailFirst report of grapevine rupestris vein feathering virus in grapevine
Khalili, Maryam; Nourinejhad Zarghani, Shaheen; Massart, Sébastien ULiege et al

in Journal of Plant Pathology (2020)

Detailed reference viewed: 30 (3 ULiège)
Full Text
See detailMaltose and Totally Impermeable Film Enhanced Suppression of Anaerobic Soil Disinfestation on Soilborne Pathogens and Increased Strawberry Yield
Song, Zhaoxin ULiege; Yan, Dongdong; Fang, Wensheng et al

in Sustainability (2020), 12(5456),

Detailed reference viewed: 27 (3 ULiège)
Full Text
See detailIdentification and molecular characterization of a novel foveavirus from Rubus spp. in Turkey
Gazel, Mona; Roumi, Vahid; Ordek, Kivilcim et al

in Virus Research (2020), 286

A novel plant virus was identified by high-throughput sequencing analysis from a raspberry plant showing slight mottling symptom. The complete genome sequence of this virus is 8645 nucleotides long ... [more ▼]

A novel plant virus was identified by high-throughput sequencing analysis from a raspberry plant showing slight mottling symptom. The complete genome sequence of this virus is 8645 nucleotides long, including the 5′ and 3′ UTRs. Its genome contains five ORFs and is very close to members of the genus Foveavirus (Quinvirinae, Betaflexiviridae) in terms of genome organization, TGB presence and the sizes of the RdRp and CP proteins. The novel virus shares 33.5–51.3 % and 23.3–41.3 % nucleotide identity to other genera of the Betaflexifiviridae family based on polymerase (RdRp) and CP genes, respectively. Compared to other foveavirus species, the RdRp protein showed the highest sequence identity (45.3 %) to the RdRp of peach chlorotic mottle virus (PCMV) while the maximal sequence identity for the CP protein was 33.9 % with grapevine rupestris stem pitting-associated virus (GRSPaV). The low nucleotide and amino acid sequence identity with known foveaviruses indicated that it was a novel virus, for which the provisional name “rubus virus 1 (RuV1)” is proposed. The phylogenetic analysis supports the assignment of this virus as a new species of the genus Foveavirus. A survey of 537 Rubus spp. samples grown in six provinces of Turkey, including some symptomatic samples, showed a RuV1 prevalence of 2.2 %, confirming its presence in both raspberry and blackberry plants in a single province, although no obvious association between virus infection and specific symptoms was found. [less ▲]

Detailed reference viewed: 26 (2 ULiège)
Full Text
See detailStandardization of Plant Microbiome Studies: Which Proportion of the Microbiota is Really Harvested?
Sare, Abdoul Razack ULiege; Stouvenakers, Gilles ULiege; Eck, Mathilde ULiege et al

in Microorganisms (2020), 8(3), 342

Studies in plant-microbiome currently use diverse protocols, making their comparison difficult and biased. Research in human microbiome have faced similar challenges, but the scientific community proposed ... [more ▼]

Studies in plant-microbiome currently use diverse protocols, making their comparison difficult and biased. Research in human microbiome have faced similar challenges, but the scientific community proposed various recommendations which could also be applied to phytobiome studies. Here, we addressed the isolation of plant microbiota through apple carposphere and lettuce root microbiome. We demonstrated that the fraction of the culturable epiphytic microbiota harvested by a single wash might only represent one-third of the residing microbiota harvested after four successive washes. In addition, we observed important variability between the efficiency of washing protocols (up to 1.6-fold difference for apple and 1.9 for lettuce). QIIME2 analysis of 16S rRNA gene, showed a significant difference of the alpha and beta diversity between protocols in both cases. The abundance of 76 taxa was significantly different between protocols used for apple. In both cases, differences between protocols disappeared when sequences of the four washes were pooled. Hence, pooling the four successive washes increased the alpha diversity for apple in comparison to a single wash. These results underline the interest of repeated washing to leverage abundance of microbial cells harvested from plant epiphytic microbiota whatever the washing protocols, thus minimizing bias. [less ▲]

Detailed reference viewed: 60 (14 ULiège)
Full Text
See detailFirst report of Grapevine pinot gris virus (GPGV) and Grapevine rupestris stem pitting-associated virus (GRSPaV) in grapevine in Belgium
Massart, Sébastien ULiege; Vankerkoven, Laetitia; Blouin, Arnaud ULiege et al

in Plant Disease (2020)

Grapevine pinot gris virus (GPGV) belongs to the genus Trichovirus of the family Betaflexiviridae from the order Tymovirales. It was first discovered in Italy in 2012, and later in a number of countries ... [more ▼]

Grapevine pinot gris virus (GPGV) belongs to the genus Trichovirus of the family Betaflexiviridae from the order Tymovirales. It was first discovered in Italy in 2012, and later in a number of countries including Germany and France. Grapevine rupestris stem pitting-associated virus (GRSPaV) is a member of the genus Foveavirus of the family Betaflexiviridae, order Tymovirales. As grapevine is known to be a host of a wide variety of viruses, a pilot sampling (10 symptomless plants) was carried out in May 2018 and the presence of GPGV evaluated by RT-PCR (data not shown). Subsequently, a leaf sample from a GPGV positive Vitis vinifera ´Regent´ from a Belgian vineyard (province of Namur) was analyzed by high-throughput sequencing (HTS). The sequencing library was prepared on the template of ribosomal-depleted total RNAs (Ribo-Zero Plant Leaf Kit, Illumina) using the TruSeq Stranded Total RNA Library Prep Kit (Illumina). The sample was sequenced (2 × 150 nt) on the Illumina Nextseq 500 platform (GIGA, Liege University, Liege, Belgium). After quality control and elimination of duplicate reads, 2,934,997 high quality paired reads were assembled de novo using SPADES as a plugin in Geneious version 10.1.5 (https://www.geneious.com). BLASTn analysis of the contigs against the NCBI reference database showed homologies to two grapevine viruses, GPGV (NC_015782.2) and GRSPaV (NC_001948.1). The nearly full genome sequences (7,344 nt for GPGV, and 8,711 nt for GRSPaV) of both viruses were reconstructed by de novo assembly, and deposited in GenBank under the accession numbers MN228488 (GPGV) and MN228487 (GRSPaV). BLASTn analysis indicated that the closest sequences to the GPGV isolate were KM491305 (France), KU194413 (Canada) and KR528581 (Korea) with 98.6% identity, while the closest sequences to the GRSPaV isolate (98.9% identity) were MG938325, MG938334 and MG938327 (France), all belonging to the molecular clade 3 of group L of GRSPaV (Hily et al. 2018). To estimate the occurrence of GPGV and GRSPaV in the vineyard, 49 samples, all asymptomatic, were randomly selected and tested together with the sequenced sample using reverse transcription polymerase chain reaction (RT-PCR). For the detection of GPGV, the primer pair GPG-14F (5’-AATTGATCCCGTGTAGTGC-3’) and GPG-632R (5’-TCCGAGGACGATGAACCTC-3’) was applied (anticipated amplicon size: 618 bp); whereas for the detection of GRSPaV, the primers GRSPV-NGS-Be-s (5’-TCTGCATTAGGCATCATGTG-3’) and GRSPV-NGS-Be-as (5’-GGCCGTTACCAATCTTCTCG-3’) were designed based on the HTS-generated sequences and used (anticipated amplicon size: 420 bp). Among the 50 samples, eight samples tested positive for GPGV, and 12 tested positive for GRSPaV. Interestingly, four samples, including the HTS-sequenced sample, were positive for both viruses. To confirm the identity of the PCR products, amplicons from two samples for each virus were sequenced at Starseq, Mainz, Germany. The sequences of the projected GPGV amplicons (accession numbers MK533603 and MK533604) showed 98.5 and 100% identity to the HTS sequence, respectively, confirming the presence of the virus in the samples. The sequences of the projected GRSPaV amplicons (MK569516 and MK569517) showed 100% identity to each other and 98.5% identity to the isolate 34 clone 1 from France (MG938303), but they diverged from the HTS sequence (80% identity). The results suggest the presence of divergent isolates of GRSPaV that belong to at least two distinct clusters (clade 1 and clade 3 in Hily et al. 2018) in the vineyard. To our knowledge, this is the first report of grapevine viruses in Belgium. Although no detrimental effects were observed on the original plant and the two viruses are very common worldwide, GPGV can be associated with severe symptoms. Unveiling the presence of the viruses in Belgium shall contribute to understanding the occurrence of the viruses and developing management measures should they become necessary. [less ▲]

Detailed reference viewed: 40 (3 ULiège)
Full Text
See detailAquaponic water, a novel source of biocontrol agents against pythium aphanidermatum root rot in lettuce
Stouvenakers, Gilles ULiege; Massart, Sébastien ULiege; Jijakli, Haissam ULiege

Poster (2020, January 31)

In aquaponics, phytosanitary treatments for plant diseases management are a delicate matter because of the presence of fish. However, it appears that aquaponic systems could be naturally armed against ... [more ▼]

In aquaponics, phytosanitary treatments for plant diseases management are a delicate matter because of the presence of fish. However, it appears that aquaponic systems could be naturally armed against plant pathogens. This natural protective action is called suppressiveness. Aquaponic water has shown a direct inhibitory effect on in vitro Pythium spp. growth (Gravel et al. 2015; Sirakov et al. 2016; Stouvenakers et al. 2018). To confirm this discovery, in vivo experiments using Pythium aphanidermatum have been carried out on lettuces growing in hydroponic (HP) water, aquaponic (AP) water or aquaponic water complemented (COMP) with mineral nutrients to reach HP levels. Suppressiveness property of AP, COMP and HP waters was evaluated by comparing root symptoms and lettuces yields. For each treatments, lettuces root microbiota were analysed after Illumina high throughput sequencing of ITS and 16S rDNA genes. Results showed that yields and roots health of AP lettuces were significantly better compared to COMP and HP lettuces. The bioinformatics analysis of sequenced microbiota showed differences in terms of microbiota diversity and features composition when comparing AP with COMP and HP water. These results highlight that the physicochemical modifications of the AP water to make the COMP water disturb the AP microbiota and lead to the loss of its suppressive capacity. Moreover, AP could be an interesting source of novel biocontrol agents for plant pathogens control in aquaponics. [less ▲]

Detailed reference viewed: 118 (6 ULiège)
Full Text
See detailSanitizing the international banana germplasm collection for a safe exchange of banana plants around the world
Berhal, Chadi ULiege; Van denhouwe, Ines; Roux, Nicolas et al

Poster (2020, January 31)

Banana is a perennial herbaceous plant and is important for food security, feeding millions of small holders, and as a cash crop in many developing countries. Preserving the biodiversity of Musa species ... [more ▼]

Banana is a perennial herbaceous plant and is important for food security, feeding millions of small holders, and as a cash crop in many developing countries. Preserving the biodiversity of Musa species is a key issue to address current and future challenges caused by abiotic or biotic stresses, among which the viral pathogens. The most comprehensive repository of Musa genetic resources in the world is managed by Bioversity International at the KULeuven, Belgium. These resources are distributed around the world and it is therefore of utmost importance to guarantee the clean health status of the plants. Some viral pathogens, like banana bunchy top virus, banana mild mosaic virus or banana streak virus have an important impact on the banana production. Therefore, the indexing and sanitation program established at the Germplasm Health Unit (GHU) at GxABT (ULiege), is screening and treating the plants from the Genebank collection held by Bioversity International. It consists of detecting the presence of infecting viruses in the plant material, and to further perform virus-cleaning therapies to eradicate the virus(es), putting back viral-free banana accessions for a safe and sustained worldwide distribution. More than 260 banana accessions entered the therapy programs, 52% of them were already send back virus-free to the Genebank, and 31% are ongoing. The protocols used, their performance as well as their adaptation to the genetic diversity of Musa will be presented and discussed. [less ▲]

Detailed reference viewed: 71 (5 ULiège)
See detailExploring the virus richness and diversity in contrasted plant communities of Poaceae in Belgium
Maclot, François ULiege; Candresse, Thierry; Filloux, Denis et al

Poster (2020, January 31)

Prior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism ... [more ▼]

Prior to plant domestication, it is hypothesized that plant viruses were co-evolving with uncultivated plants growing in mixed species communities, thereby resulting in complex interactions (antagonism, commensalism, mutualism). Development of agriculture further deeply modified natural ecosystems, land use and dynamics of virus-plant interactions, which has fostered occurrence of virus disease emergence events. In this context, we are conducting a study in the Natural Park “Burdinale-Mehaigne” (Belgium) using high throughput sequencing technologies in order to examine the impact of plant species diversity on the virome (e.g. the genomes of viral community) of Poaceae in contrasted plant communities (cereal monocultures, grazed pastures and natural grasslands). We adapted a virion-associated nucleic acids (VANA) metagenomics protocol to sequence at high throughput pools of 50 plant samples per plant community (50 samples reflecting plant species composition) and per plant species. Over two years, about 4,300 Poaceae plants (corresponding to 24 species) were sampled and bioinformatic analyses revealed presence of diverse viral communities in wild and cultivated Poaceae, even though they did not present any symptoms. These viruses belong to diverse families (e.g. Alphaflexiviridae, Luteoviridae, Partitiviridae, Totiviridae), infecting a large range of hosts within Poaceae and transmitted by different vectors (insects, mites, nematods) or seed-borne. More than 30 virus species were detected in the different plots and at least half of them are candidates for new virus species, revealing that virome in Poaceae-based agroecosystems and its impact on the dynamic of plant communities remain largely unexplored. [less ▲]

Detailed reference viewed: 89 (4 ULiège)
See detailGenebank Platform: GreenPass puts germplasm distribution in the fast lane
Massart, Sébastien ULiege; Major, Michael; Kumar, Lava

E-print/Working paper (2020)

Detailed reference viewed: 23 (1 ULiège)
Full Text
See detailComplete Sequence, Genome Organization and Molecular Detection of Grapevine Line Pattern Virus, a New Putative Anulavirus Infecting Grapevine
Elbeaino, Toufic; Kontra, Levente; Demian, Emese et al

in Viruses (2020), 12(602),

Detailed reference viewed: 23 (5 ULiège)
Full Text
See detailSixty Years After the First Description: Genome Sequence and Biological Characterization of European Wheat Striate Mosaic Virus Infecting Cereal Crops
Sõmera, Merike; Kvarnheden, Anders; Desbiez, Cécile et al

in Phytopathology (2020)

Detailed reference viewed: 39 (10 ULiège)