Publications and communications of Patrick Fickers

Do, D., Guruk, M., Kus-Liśkiewicz, M., Damblon, C., Arguelles-Arias, A., Erten, H., & Fickers, P. (February 2024). Biosynthesis of the antioxidant γ-glutamyl-cysteine with engineered Yarrowia lipolytica. Biotechnology Journal, 19 (2), 2300564. doi:10.1002/biot.202300564

Velastegui, E., Quezada, J., Guerrero, K., Altamirano, C., Martinez Alvarez, J. A., Berrios, J., & Fickers, P. (April 2023). Is heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris? Applied Microbiology and Biotechnology, 107 (7-8), 2223 - 2233. doi:10.1007/s00253-023-12434-2

Bustos Cosios, C. V., Fickers, P., & Berrios, J. (10 February 2023). CELL ENGINEERING OF THE KOMAGATAELLA PHAFFII FOR CO-SUBSTRATE METABOLISM (SORBITOL) [Poster presentation]. Annual BSM Symposium 2023 - Amazing Microbes, Brussels, Belgium.

Berrios, J., Theron, C., Steels, S., Ponce, B., Velastegui, E., Bustos Cosios, C. V., Altamirano, C., & Fickers, P. (20 July 2022). Role of Dissimilative Pathway of Komagataella phaffii (Pichia pastoris): Formaldehyde Toxicity and Energy Metabolism. Microorganisms, 10 (7), 1466. doi:10.3390/microorganisms10071466

Bustos Cosios, C. V., Fickers, P., & Berrios, J. (22 June 2022). S7_P1 CELL ENGINEERING OF THE KOMAGATAELLA PHAFFII FOR CO-SUBSTRATE METABOLISM [Poster presentation]. 1 st Polish Yeast Conference, Rzeszów, Poland.

Bustos Cosios, C. V., Quezada, J., Veas, R., Altamirano, C., Braun-Galleani, S., Fickers, P., & Berrios, J. (14 April 2022). Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production. Metabolites, 12 (4), 346. doi:10.3390/metabo12040346

Ben Amira, A., Arguelles Arias, A., Fickers, P., Attia, H., Besbes, S., & Blecker, C. (2021). Effect of brine concentration on physico-chemical characteristics, texture, rheological properties and proteolysis level of cheeses produced by an optimized wild cardoon rennet. Journal of Food Science and Technology. doi:10.1007/s13197-020-04643-8

Do, D. T. H., Fickers, P., & Ben Tahar, I. (2021). Improvement of glutathione production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. Biotechnology Letters, 43 (2), 407-414. doi:10.1007/s10529-020-03039-0

Li, C., Ong, K. L., Cui, Z., Sang, Z., Li, X., Patria, R. D., Qi, Q., Fickers, P., Yan, J., & Lin, C. S. K. (2021). Promising advancement in fermentative succinic acid production by yeast hosts. Journal of Hazardous Materials, 401. doi:10.1016/j.jhazmat.2020.123414

Nunes, P. M. B., Fraga, J. L., Ratier, R. B., Rocha-Leão, M. H. M., Brígida, A. I. S., Fickers, P., & Amaral, P. F. F. (2021). Waste soybean frying oil for the production, extraction, and characterization of cell-wall-associated lipases from Yarrowia lipolytica. Bioprocess and Biosystems Engineering. doi:10.1007/s00449-020-02489-0

Do Thi Hoang, D., & Fickers, P. (23 April 2020). Engineering Yarrowia lipolytica for the Synthesis of Glutathione from Organic By-Products. Microorganisms, 8 (4). doi:10.3390/microorganisms8040611

Carsanba, E., Papanikolaou, S., Fickers, P., & Erten, H. (2020). Lipids by yarrowia lipolytica strains cultivated on glucose in batch cultures. Microorganisms, 8 (7), 1-14. doi:10.3390/microorganisms8071054

Fickers, P. (2020). Metabolic engineering of non-conventional yeast [Paper presentation]. ESBES2020.

Fickers, P., Cheng, H., & Lin, C. S. K. (2020). Sugar alcohols and organic acids synthesis in yarrowia lipolytica: Where are we? Microorganisms, 8 (4). doi:10.3390/microorganisms8040574

Gorczyca, M., Kaźmierczak, J., Steels, S., Fickers, P., & Celińska, E. (2020). Impact of oxygen availability on heterologous geneexpression and polypeptide secretion dynamics in Yarrowia lipolytica-based protein production platforms. Yeast. doi:10.1002/yea.3499

Niang, P. M., Arguelles Arias, A., Steels, S., Denies, O., Nicaud, J.-M., & Fickers, P. (2020). In Yarrowia lipolytica erythritol catabolism ends with erythrose phosphate. Cell Biology International, 44 (2), 651-660. doi:10.1002/cbin.11265

Ong, K. L., Fickers, P., & Lin, C. S. K. (2020). Enhancing succinic acid productivity in the yeast Yarrowia lipolytica with improved glycerol uptake rate. Science of the Total Environment, 702. doi:10.1016/j.scitotenv.2019.134911

Theron, C. W., Vandermies, M., Telek, S., Steels, S., & Fickers, P. (2020). Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B. Scientific Reports, 10 (1). doi:10.1038/s41598-020-58683-3

Vassau, A., Fickers, P., Leclere, V., & Jacques, P. (2020). Overall strategy to implement NRPS production in fungal host [Paper presentation]. ESBES2020.

Vandermies, M., Theron, C., & Fickers, P. (17 September 2019). The quest for a cell factory for the production of recombinant proteins: Pichia pastoris vs Yarrowia lipolytica [Paper presentation]. ECAB5, Firenze, Italy.

Vandermies, M., & Fickers, P. (2019). Strategies at Bioreactor Scale for the Production of Recombinant Proteins in Yarrowia lipolytica. In A. Sibirny, Non-conventional Yeasts: from Basic Research to Application. Springer. doi:10.1007/978-3-030-21110-3_16

Ben Tahar, I., Kus-Liśkiewicz, M., Lara, Y., Javaux, E., & Fickers, P. (2019). Characterization of a non-toxic pyomelanin pigment produced by the yeast Yarrowia lipolytica. Biotechnology Progress. doi:10.1002/btpr.2912

Vandermies, M., Theron, C., & Fickers, P. (04 June 2019). The quest for the best cell factory for recombinant protein production: Yarrowia lipolytica vs Pichia pastoris [Poster presentation]. Emerging applications of microbes, Leuven, Belgium.

Vassaux, A., Tarayre, C., Arguelles Arias, A., Compère, P., Delvigne, F., Fickers, P., Jahn, L., Lang, A., Leclère, V., Ludwig-Müller, J., Ongena, M., Schafhauser, T., Telek, S., Théatre, A., van Berkel, W., Vandenbol, M., van Pée, K.-H., Willems, L., Wohlleben, W., & Jacques, P. (2019). Astin C production by the endophytic fungus Cyanodermella asteris in planktonic and immobilized culture conditions. Biotechnology Journal. doi:10.1002/biot.201800624

Do Thi Hoang Diem, Steels, S., Denies, O., & Fickers, P. (08 May 2019). By-products show potential in glutathione production by engineered non-conventional yeast Yarrowia lipolytica [Poster presentation]. 5th Edition of The international conferences Green Chemistry and White biotechnology on (BIO-) Polimers and Ecocircularly, Chaleroi, Belgium.

Do Thi Hoang Diem, Vandermies, M., Fickers, P., & Theron, C. (2019). Non-Conventional Yeast Species for Recombinant Protein and Metabolite Production. In Reference module in Life Sciences. Elsevier.

Vancsok, C., & Fickers, P. (2019). Un édulcorant dans votre café ?

Vandermies, M., & Fickers, P. (30 January 2019). Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica. Microorganisms, 7 (2), 40. doi:10.3390/microorganisms7020040

Ben Tahar, I., Fickers, P., Dziedzic, A., Płoch, D., Skóra, B., & Kus-Liśkiewicz, M. (2019). Green pyomelanin-mediated synthesis of gold nanoparticles: Modelling and design, physico-chemical and biological characteristics. Microbial Cell Factories, 18 (1). doi:10.1186/s12934-019-1254-2

Carsanba, E., Papanikolaou, S., Fickers, P., Agirman, B., & Erten, H. (2019). Citric acid production by yarrowia lipolytica. In A. Sibirny (Ed.), Non-conventional Yeasts: from Basic Research to Application (pp. 91-117). Springer International Publishing. doi:10.1007/978-3-030-21110-3_4

Carsanba, E., Papanikolaou, S., Fickers, P., & Erten, H. (2019). Screening various Yarrowia lipolytica strains for citric acid production. Yeast, 36 (5), 319-327. doi:10.1002/yea.3389

Chi, P., Wang, S., Ge, X., Bilal, M., Fickers, P., & Cheng, H. (2019). Efficient D-threitol production by an engineered strain of Yarrowia lipolytica overexpressing xylitol dehydrogenase gene from Scheffersomyces stipitis. Biochemical Engineering Journal, 149. doi:10.1016/j.bej.2019.107259

Do Thi Hoang Diem, Theron, C., & Fickers, P. (2019). Organic wastes as feedstocks for non-conventional yeast-based bioprocesses. Microorganisms, 7 (8). doi:10.3390/microorganisms7080229

Fickers, P. (2019). The quest for the best cell factory for the production of recombinant proteins: Pichia pastoris vs Yarrowia lipolytica [Paper presentation]. 6th Ukrainian Congress for Cell Biology, Ukraine.

Fickers, P. (2019). Erythritol metabolism in Yarrowia lipolytica and engineering tools derived [Paper presentation]. ISSY35.

Ong, K., Fickers, P., Pank, K., Dharma, P., Luk, H., Uisan, K., & Lin, C. (2019). Fermentation of fruit and vegetable wastes for biobased products. In R. Kosseva & C. Webb, Food industry wastes: assessment and recuperation of comodities. Academic Press.

Park, Y.-K., Vandermies, M., Soudier, P., Telek, S., Thomas, S., Nicaud, J.-M., & Fickers, P. (2019). Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions. Microbial Cell Factories, 18 (1). doi:10.1186/s12934-019-1218-6

Theron, C. W., Berrios, J., Steels, S., Telek, S., Lecler, R., Rodriguez, C., & Fickers, P. (2019). Expression of recombinant enhanced green fluorescent protein provides insight into foreign gene-expression differences between Mut+ and MutS strains of Pichia pastoris. Yeast (Chichester, England), 36 (5), 285-296. doi:10.1002/yea.3388

Vassaux, A., Meunier, L., Vandenbol, M., Baurain, D., Fickers, P., Jacques, P., & Leclère, V. (2019). Nonribosomal peptides in fungal cell factories: from genome mining to optimized heterologous production. Biotechnology Advances. doi:10.1016/j.biotechadv.2019.107449

Velastegui, E., Theron, C., Berrios, J., & Fickers, P. (2019). Downregulation by organic nitrogen of AOX1 promoter used for controlled expression of foreign genes in the yeast Pichia pastoris. Yeast, 36 (5), 297-304. doi:10.1002/yea.3381

Nicaud, J.-M., Trassaert, M., Thomas, S., Fickers, P., Vandermies, M., & Carly, F. (22 November 2018). Inducible promoter for gene expression and synthetic biology.

Vandermies, M., & Fickers, P. (10 September 2018). Metabolic engineering of Yarrowia lipolytica for taurine synthesis [Paper presentation]. 12th Symposium of the European Society of Biochemical Engineering Sciences, Lisbon, Portugal.

Bouchedja D. N., Danthine, S., Kar, T., Fickers, P., Sassi H., Boudjellal A., Blecker, C., & Delvigne, F. (04 July 2018). pH level has a strong impact on population dynamics of the yeast Yarrowia lipolytica and oil micro-droplets in multiphasic bioreactor. FEMS Microbiology Letters, 365 (6). doi:10.1093/femsle/fny173

Vandermies, M., Kar, T., Carly, F., Nicaud, J.-M., Delvigne, F., & Fickers, P. (2018). Yarrowia lipolytica morphological mutant enables lasting in situ immobilization in bioreactor. Applied Microbiology and Biotechnology. doi:10.1007/s00253-018-9006-5

Vandermies, M., Denies, O. (Other coll.), Do, H. (Other coll.), Theron, C. (Other coll.), Vassaux, A. (Other coll.), & Fickers, P. (Other coll.). (2018). La levure comme usine cellulaire.

Vandermies, M., Kar, T., Carly, Nicaud, J.-M., Delvigne, F., & Fickers, P. (17 May 2018). New filamentous mutant of Yarrowia lipolytica and its use in biofilm bioreactors [Poster presentation]. Bioforum Liège 2018.

Ben Amira, A., Blecker, C., Richel, A., Fickers, P., Francis, F., Besbes, S., & Attia, H. (18 March 2018). Influence of the ripening stage and the lyophilization of wild cardoon flowers on their chemical composition, enzymatic activities of extracts and technological properties of cheese curds [Poster presentation]. 6è Journées scientifiques de l'Agro-Alimentaire, Sousse, Tunisia.

Vandermies, M., Denies, O., Nicaud, J.-M., & Fickers, P. (08 February 2018). Catabolic selectable marker eases genome editing in Yarrowia lipolytica [Paper presentation]. National Symposium for Applied Biotechnological Sciences 2018, Bruxelles, Belgium.

Vandermies, M., Trassaert, M., Carly, F., Denies, O., Thomas, S., Fickers, P., & Nicaud, J.-M. (08 February 2018). Versatile regulated promoter for Yarrowia lipolytica [Poster presentation]. National Symposium for Applied Biotechnological Sciences 2018, Bruxelles, Belgium.

Ben Amira, A., Blecker, C., Richel, A., Arguelles Arias, A., Fickers, P., Francis, F., Besbes, S., & Attia, H. (2018). Influence of the ripening stage and the lyophilization of wild cardoon flowers on their chemical composition, enzymatic activities of extracts and technological properties of cheese curds. Food Chemistry, 245, 919-925. doi:10.1016/j.foodchem.2017.11.082

Carly, F., & Fickers, P. (2018). Erythritol production by yeasts: A snapshot of current knowledge. Yeast. doi:10.1002/yea.3306

Carly, F., Steels, S., Telek, S., Vandermies, M., Nicaud, J.-M., & Fickers, P. (2018). Identification and characterization of EYD1, encoding an erythritol dehydrogenase in Yarrowia lipolytica and its application to bioconvert erythritol into erythrulose. Bioresource Technology, 247, 963-969. doi:10.1016/j.biortech.2017.09.168

Cheng, H., Wang, S., Bilal, M., Ge, X., Zhang, C., Fickers, P., & Cheng, H. (2018). Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowia lipolytica and improvement of erythritol productivity using metabolic engineering. Microbial Cell Factories, 17 (1), 133. doi:10.1186/s12934-018-0982-z

da Silva, L. V., Coelho, M. A. Z., Amaral, P. F. F., & Fickers, P. (2018). A novel osmotic pressure strategy to improve erythritol production by Yarrowia lipolytica from glycerol. Bioprocess and Biosystems Engineering, 41 (12), 1883-1886. doi:10.1007/s00449-018-2001-5

Delvigne, F., Zacchetti, B., Fickers, P., Fifani, B., Roulling, F., Lefebvre, C., Neubauer, P., & Junne, S. (2018). Improving control in microbial cell factories: from single-cell to large-scale bioproduction. FEMS Microbiology Letters, 365 (22). doi:10.1093/femsle/fny236

Fickers, P. (2018). The yeast Yarrowia lipolytica as a cell factory [Paper presentation]. CBM2018.

Fickers, P. (2018). Recent insights on pAOX1 regulation in Komagataella pastoris [Paper presentation]. International conference on advances in microbiology and biotechnology.

Fickers, P. (2018). New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica [Paper presentation]. NCY2018.

Theron, C., Berrios, J., Delvigne, F., & Fickers, P. (2018). Integrating metabolic modeling and population heterogeneity analysis into optimizing recombinant protein production by Komagataella (Pichia) pastoris. Applied Microbiology and Biotechnology, 102 (1), 63-80. doi:10.1007/s00253-017-8612-y

Yurkiv, M., Kurylenko, O., Vasylyshyn, R., Dmytruk, K., Fickers, P., & Sibirny, A. (2018). Gene of the transcriptional activator MET4 is involved in regulation of glutathione biosynthesis in the methylotrophic yeast Ogataea (Hansenula) polymorpha. FEMS Yeast Research, 18 (2). doi:10.1093/femsyr/foy004

Carly, F., Vandermies, M., Telek, S., Steels, S., Thomas, S., Nicaud, J.-M., & Fickers, P. (September 2017). Metabolic engineering of Yarrowia lipolytica for the synthesis of added value chemicals [Paper presentation]. 7th International Weigl Conference, Ukraine.

Vandermies, M., Denies, O., Nicaud, J.-M., & Fickers, P. (September 2017). Useful tools for genome editing in the non-conventional yeast Yarrowia lipolytica [Poster presentation]. 7th International Weigl Conference, Lviv, Ukraine.

Vandermies, M., Kar, T., Denies, O., Nicaud, J.-M., & Fickers, P. (September 2017). New filamentous mutant of Yarrowia lipolytica and its use in biofilm bioreactors [Poster presentation]. 7th International Weigl Conference, Lviv, Ukraine.

Vandermies, M., Denies, O., Nicaud, J.-M., & Fickers, P. (August 2017). EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica. Journal of Microbiological Methods, 139, 161-164. doi:10.1016/j.mimet.2017.05.012

Carly, F., Vandermies, M., Telek, S., Steels, S., Thomas, S., Nicaud, J.-M., & Fickers, P. (July 2017). Enhancing erythritol productivity in Yarrowia lipolytica using metabolic engineering. Metabolic Engineering, 42, 19-24. doi:10.1016/j.ymben.2017.05.002

Carly, F., Vandermies, M., Nicaud, J.-M., & Fickers, P. (June 2017). Enhancing the sweetener erythritol productivity in Yarrowia lipolytica using metabolic engineering [Paper presentation]. 33rd International Specialised Symposium on Yeast, Cork, Ireland.

Vassaux, A., Tarayre, C., Delvigne, F., Fickers, P., Jacques, P., Vandenbol, M., & Leclere, V. (24 May 2017). An anticancer molecule stemming from a plant root produced in a bioreactor [Poster presentation]. Terra Innovation Game.

Ben Taher, I., Bennour, H., Fickers, P., & Hassouna, M. (2017). Valorization of Potato Peels Residues on Cellulase Production Using a Mixed Culture of Aspergillus niger ATCC 16404 and Trichoderma reesei DSMZ 970. Waste and Biomass Valorization, 8 (1), 183-192. doi:10.1007/s12649-016-9558-5

Ben Taher, I., Fickers, P., Chniti, S., & Hassouna, M. (2017). Optimization of enzymatic hydrolysis and fermentation conditions for improved bioethanol production from potato peel residues. Biotechnology Progress, 33 (2), 397-406. doi:10.1002/btpr.2427

Bouchedja, D. N., Danthine, S., Kar, T., Fickers, P., Boudjellal, A., & Delvigne, F. (2017). Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775. Bioresources and Bioprocessing, 4 (3), 1-12. doi:10.1186/s40643-016-0132-6

Carly, F., Gamboa-Melendez, H., Vandermies, M., Damblon, C., Nicaud, J.-M., & Fickers, P. (2017). Identification and characterization of EYK1, a key gene for erythritol catabolism in Yarrowia lipolytica. Applied Microbiology and Biotechnology, 101. doi:10.1007/s00253-017-8361-y

Carly, F., Gamboa-Melendez, H., Vandermies, M., Damblon, C., Nicaud, J. M., & Fickers, P. (2017). Erratum to: Identification and characterization of EYK1, a key gene for erythritol catabolism in Yarrowia lipolytica (Applied Microbiology and Biotechnology, (2017), 10.1007/s00253-017-8361-y). Applied Microbiology and Biotechnology, 101 (16), 6573. doi:10.1007/s00253-017-8412-4

Delvigne, F., Baert, J., Sassi, H., Fickers, P., Grünberger, A., & Dusny, C. (2017). Taking control over microbial populations: Current approaches for exploiting biological noise in bioprocesses. Biotechnology Journal, 12 (7). doi:10.1002/biot.201600549

Fickers, P. (2017). The yeast Yarrowia lipolytica as a cell-factory for the synthesis of added-value chemicals [Paper presentation]. Microbial Physiology and Genomics 2017.

Fickers, P. (2017). Pichia process optimisation by methanol/sorbitol co-feeding [Paper presentation]. 7th IFIBIOP, China.

Fickers, P. (2017). Metabolic engineering of Yarrowia lipolytica for the synthesis of added value chemicals [Paper presentation]. 7th International Weigl Conference.

Nicaud, J., Fickers, P., & Carly, F. (2017). Mutant yeast strains with enhanced production of erythritol or erythrulose.

Sassi, H., Delvigne, F., Kallel, H., & Fickers, P. (2017). pH and Not Cell Morphology Modulate pLIP2 Induction in the Dimorphic Yeast Yarrowia lipolytica [letter to the editor]. Current Microbiology, 74 (3), 413-417. doi:10.1007/s00284-017-1207-0

Trassaert, M.* , Vandermies, M.* , Carly, F., Denies, O., Thomas, S., Fickers, P., & Nicaud, J.-M. (2017). New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica. Microbial Cell Factories, 16 (141). doi:10.1186/s12934-017-0755-0
* These authors have contributed equally to this work.

Fickers, P. (September 2016). Decihering LIP2 and POX2 promoter regulation for high yield recombinant proteins production in the yeast Yarrowia lipolytica [Paper presentation]. ICY2016.

Baert, J., Delepierre, A., Telek, S., Fickers, P., Toye, D., de Lamotte, A., Lara, A. R., Jaén, K. E., Gosset, G., Jensen, P. R., & Delvigne, F. (2016). Microbial population heterogeneity versus bioreactor heterogeneity: Evaluation of Redox Sensor Green as an exogenous metabolic biosensor. Engineering in Life Sciences, 16 (7), 643 - 651. doi:10.1002/elsc.201500149

Carly, F., Delvigne, F., Kar, T., Nicaud, J., Crutz-Le Coq, A., Steels, S., & Fickers, P. (2016). Pichia process optimisation by methanol/sorbitol co-feeding [Paper presentation]. 7th World Congress and Expo on Applied Microbiology, Dubai, United Arab Emirates.

Carly, F., Niu, H., Delvigne, F., & Fickers, P. (2016). Influence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with limited oxygen transfer rate. Journal of Industrial Microbiology and Biotechnology, 43 (4), 517-23. doi:10.1007/s10295-015-1722-6

Sassi, H., Delvigne, F., Nicaud, J.-M., Crutz Le Coq, A.-M., Steels, S., & Fickers, P. (2016). Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica. Microbial Cell Factories, 15 (1), 159. doi:10.1186/s12934-016-0558-8

Beye, C., Tounkara, L. S., Seck, M. A., Thonart, P., & Fickers, P. (November 2015). Opportunités pour la valorisation des végétaux riches en anthocyanes comme sources de colorants alimentaires (synthèse bibliographique). Biotechnologie, Agronomie, Société et Environnement, 19 (4), 392 - 401.

Baert, J., Kinet, R., Brognaux, A., Delepierre, A., Telek, S., Sorensen, S. J., Riber, L., Fickers, P., & Delvigne, F. (2015). Phenotypic variability in bioprocessing conditions can be tracked on the basis of on-line flow cytometry and fits to a scaling law. Biotechnology Journal. doi:10.1002/biot.201400537

Rakicka, M., Lazar, Z., Dulermo, T., Fickers, P., & Nicaud, J. M. (2015). Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions. Biotechnology for Biofuels, 8, 104. doi:10.1186/s13068-015-0286-z

Sanchez-Vargas, E., Alcala-Galiano, D., Fickers, P., Chico, E., & Ramos-Sanchez, L. (2015). Use of selectivity factor on the conceptual design of cell culture systems. Biotecnologia Aplicada.

Ramos-Sanchez, L. B., Julian-Ricardo, M. C., Cordova, J., & Fickers, P. (December 2014). Fungal lipase production by solid-state fermentation [Paper presentation]. Biotecnología Habana.

Fickers, P. (November 2014). Bacillus as a source of novel antibiotics [Paper presentation]. Bacteriology 2014, Chicago, United States.

Ramos-Sanchez, L. B., Cujilema-Quitio, M. C., Leon-Revelo, G., Marquez-Penamaria, G., Morell-Napoles, G., & Fickers, P. (November 2014). Propuesta de estrategia para la producción de biocombustibles de biomasa lignocelulósica [Paper presentation]. 4to Taller Nacional de Fuentes Renovables de Energía, Camaguey, Cuba.

Carly, F., Niu, H., & Fickers, P. (October 2014). Optimization of pAOX1 induction in a methanol/sorbitol co-feeding process in Pichia pastoris [Paper presentation]. 31st International Specialised Symposium on Yeast, Vipava, Slovenia.

Carly, F., Niu, H., & Fickers, P. (September 2014). Optimization of pAOX1 induction in a methanol/sorbitol co-feeding process in Pichia pastoris Mut+/pAOX1-lacZ strain [Paper presentation]. ESBES-IFIBIOP 2014 - 10th European Symposium on Biochemical Engineering Sciences and 6th International Forum on Industrial Bioprocesses, Lille, France.

Arguelles Arias, A., Joris, B., & Fickers, P. (21 April 2014). Dual Mode of Action of Amylolysin, a Type-B Lantibiotic Produced by Bacillus amyloliquefaciens GA1. Protein and Peptide Letters, 4, 336-340. doi:10.2174/09298665113206660102

Bouchedja, D. N., Delvigne, F., Boudjellal, A., Blecker, C., Kar, T., Fickers, P., & Danthine, S. (06 April 2014). Accumulation lipidique par Yarrowia lipolytica: un nouvel outil d'observation [Paper presentation]. 2ème journées Scientifiques de l'Agro-Alimentaire (J.S.A.A. 2014), Sousse, Tunisia.

Fickers, P. (April 2014). Antibiotic compounds from Bacillus: why are they so amazing? [Paper presentation]. 5th International Young Scientist Conference on Biotechnology for Sustainable Development, Rzeszow, Poland.

Fickers, P. (2014). Pichia pastoris: a workhorse for recombinant protein production. Current Research in Microbiology and Biotechnology, (2), 354-363.

Jacques, P., Coutte, F., Bechet, M., Guez, J. S., Leclere, V., Dhali, D., Fickers, P., John, M., & Niehren, J. (2014). Directed Biosynthesis of Antifungal and Biosurfactant Lipopeptides from Bacillus subtilis [Paper presentation]. 10th European Symposium on Biochemical Engineering Science, Lille, France.

Richelle, A., Fickers, P., & Bogaerts, P. (2014). Macroscopic modelling of baker’s yeast production in fed-batch cultures and its link with trehalose production. Computers and Chemical Engineering, (61), 220-233. doi:10.1016/j.compchemeng.2013.11.007

Sibirny, A., Madzak, K., & Fickers, P. (2014). Genetic engineering of non-conventional yeast for the production of valuable Compounds. In F. Darvishi, Microbial biotechnology, Progress and trends. CRC Press.

Fickers, P. (September 2013). The lipases from Y. lipolytica: genetics, production, regulation, biochemical characterization and biotechnological applications [Paper presentation]. Yeast 2013, Frankfurt, Germany.

Schyns, G., Serra, C., Henriques, A., Arguelles Arias, A., Joris, B., & Fickers, P. (22 July 2013). ISOLATION OF THE ANTIMICROBIAL CYCLIC PEPTIDE SUBTILOSIN A FROM A GUT-ASSOCIATED BACILLUS SUBTILIS STRAIN. American Journal of Biochemistry and Biotechnology, 9 (3). doi:10.3844/ajbbsp.2013.307.317

Arguelles Arias, A., Joris, B., Ongena, M., & Fickers, P. (June 2013). Characterisation, production and application of a novel lantibiotic from Bacillus amyloliquefaciens [Paper presentation]. XV Congreso Nacional de Biotecnología y Bioingeniería. SMBB/GIM-2013, Cancun, Mexico.

Fickers, P. (June 2013). The amylolysin from Bacillus amyloliquefaciens GA1 [Paper presentation]. V Conferencia International « Cienca y Tecnologia por un Desarrollo Sostenible » CYTDES 2013, Cuba.

Arguelles Arias, A., Ongena, M., Devreese, B., Terrak, M., Joris, B., & Fickers, P. (2013). Characterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1. PLoS ONE, 8 (12). doi:10.1371/journal.pone.0083037

Bechet, M., Castera-Guy, J., Guez, J.-S., Chihib, N.-E., Coucheney, F., Coutte, F., Fickers, P., Leclere, V., Wathelet, B., & Jacques, P. (2013). Production of a novel mixture of mycosubtilins by mutants of Bacillus subtilis. Bioresource Technology, 145, 264-70. doi:10.1016/j.biortech.2013.03.123

Fickers, P., & Nicaud, J. M. (2013). Biotechnological application of Yarrowia lipolytica lipase : an overview. In Yarrowia lipolytica: Biotechnological Applications (pp. 121-136). Springer.

Fickers, P., Sauveplane, V., & Nicaud, J. M. (2013). The lipase from Y. lipolytica : genetics, production, regulation and biochemical characterisation. In Yarrowia lipolytica: Biotechnological Applications (pp. 99-119). Springer.

Gahungu, M., Arguelles Arias, A., Fickers, P., Zervosen, A., Joris, B., Damblon, C., & Luxen, A. (2013). Synthesis and biological evaluation of potential threonine synthase inhibitors: Rhizocticin A and Plumbemycin A. Bioorganic and Medicinal Chemistry, 21 (17), 4958-67. doi:10.1016/j.bmc.2013.06.064

Jacques, P., Bechet, M., Castera-Guy, J., Chihib, N., Coutte, F., Coucheney, F., Deravel, J., Fickers, P., Guez, J. S., Hofte, M., Leclere, V., Lemiere, S., Lepretre, A., Van Hese, N., & Wathelet, B. (2013). Novel biosurfactants with biocontrol activity and low ecotoxicity [Paper presentation]. 9th European Congress of Chemical Engineering and 2nd European Congress of Applied Biotechnology, The Hague, Netherlands.

Nastro, R. A.* , Arguelles Arias, A.* , Ongena, M., Di Costanzo, A., Trifuoggi, M., Guida, M., & Fickers, P. (2013). Antimicrobial Activity of Bacillus amyloliquefaciens ANT1 Toward Pathogenic Bacteria and Mold: Effects on Biofilm Formation. Probiotics and Antimicrobial Proteins, 5, 252-258. doi:10.1007/s12602-013-9143-1
* These authors have contributed equally to this work.

Niu, H., Amribt, Z., Fickers, P., Tan, W., & Bogaerts, P. (2013). Metabolic Pathway Analysis and Reduction for Mammalian Cell Cultures-Towards Macroscopic Modeling. Chemical Engineering Science, (102), 461-473. doi:10.1016/j.ces.2013.07.034

Niu, H., Daukandt, M., Rodriguez, C., Fickers, P., & Bogaerts, P. (2013). Dynamic modeling of methylotrophic Pichia pastoris culture with exhaust gas analysis: from cellular metabolism to process simulation. Chemical Engineering Science, (87), 381-392. doi:10.1016/j.ces.2012.11.006

Niu, H., Jost, L., Pirlot, N., Sassi, H., Daukandt, M., Rodriguez, C., & Fickers, P. (2013). A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut(+)/pAOX1-lacZ strain. Microbial Cell Factories, 12, 33. doi:10.1186/1475-2859-12-33

Schyns, G., Serra, C. R., Lapointe, T., Pereira-Leal, J. B., Potot, S., Fickers, P., Perkins, J. B., Wyss, M., & Henriques, A. O. (2013). Genome of a Gut Strain of Bacillus subtilis. Genome Announcements, 1 (1). doi:10.1128/genomeA.00184-12

Arguelles Arias, A., Ongena, M., Joris, B., & Fickers, P. (September 2012). Amylolysin, an unusual lantibiotic from the soil bacterium Bacillus amyloliquefaciens with potent antilisterial activity on poultry meat [Paper presentation]. IBS 2012 - 15th International Biotechnology Symposium & Exhibition, Daegu, South Korea.

Arguelles Arias, A., Ongena, M., Fickers, P., & Joris, B. (30 August 2012). The Bacillus amyloliquefaciens lantibiotic Amylolysin : a potent weapon against multi-drug resistant pathogens [Paper presentation]. International Meeting on Antimicrobial Peptides, Leipzig, Germany.

Fickers, P. (2012). Antibiotic compounds from Bacillus: why are they so amazing. American Journal of Biochemistry and Biotechnology, (8), 38-43.

Arguelles Arias, A., Craig, M., & Fickers, P. (2011). Gram-positive antibiotic biosynthesis cluster: a review. In Science against microbial pathogens: communicating current research and technological advances. A. Mendez-Vilas.

Arguelles Arias, A., Ongena, M., Devreese, B., Joris, B., & Fickers, P. (2011). Production et purification de l’amylolysine, un lantibiotique produit par Bacillus amyloliquefaciens GA1 [Poster presentation]. 13ème Congrès de la Société Française de Génie des Procédés, Lille, France.

Cawoy, H., Bettiol, W., Fickers, P., & Ongena, M. (2011). Bacillus-based biological control of plant diseases. In M. Stoytcheva (Ed.), Pesticides in the Modern World - Pesticides Use and Management. Rijeka, Croatia: InTech.

Fickers, P., Marty, A., & Nicaud, J. M. (2011). The lipases from Yarrowia lipolytica: genetics, production, regulation, biochemical characterization and biotechnological applications. Biotechnology Advances, 29 (6), 632-44. doi:10.1016/j.biotechadv.2011.04.005

Halimi, B. E., Dortu, C., Arguelles Arias, A., Thonart, P., Joris, B., & Fickers, P. (2010). Antilisterial Activity on Poultry Meat of Amylolysin, a Bacteriocin from Bacillus amyloliquefaciens GA1. Probiotics and Antimicrobial Proteins. doi:10.1007/s12602-010-9040-9

Arguelles Arias, A., Ongena, M., Halimi, B., Lara, Y., Brans, A., Joris, B., & Fickers, P. (26 November 2009). Bacillus amyloliquefaciens GA1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens. Microbial Cell Factories, 8 (1), 63. doi:10.1186/1475-2859-8-63

Schyns, G., Pereira-Leal, J., Serra, C., Fickers, P., Potot, S., Barbosa, T., Chevreux, B., de Saizieu, A., Perkings, J., Wyss, M., & Henriques, A. (June 2009). The genome of a gut strain of Bacillus subtilis [Paper presentation]. 15th internationnal conference on Bacilli, San Diego, United States.

Dortu, C., Fickers, P., Franz, C. M. A. P., Ndagano, D., Huch, M., Holzapfel, W. H., Joris, B., & Thonart, P. (2009). Characterisation of an Antilisterial Bacteriocin Produced by Lactobacillus sakei CWBI-B1365 Isolated from Raw Poultry Meat and Determination of Factors Controlling its Production. Probiotics and Antimicrobial Proteins, 1, 75-84.

Fickers, P., Destain, J., & Thonart, P. (2009). Improvement Of Yarrowia Lipolytica Lipase Production By Fed-Batch Fermentation. Journal of Basic Microbiology, 49 (2), 212-215. doi:10.1002/jobm.200800186

Fickers, P., Guez, J.-S., Damblon, C., Leclere, V., Bechet, M., Jacques, P., & Joris, B. (2009). High-level biosynthesis of the anteiso-C(17) isoform of the antibiotic mycosubtilin in Bacillus subtilis and characterization of its candidacidal activity. Applied and Environmental Microbiology, 75 (13), 4636-40. doi:10.1128/AEM.00548-09

Nihorimbere, V., Fickers, P., Thonart, P., & Ongena, M. (Other coll.). (2009). Ecological fitness of Bacillus subtilis BGS3 regarding production of the surfactin lipopeptide in the rhizosphere. Environmental Microbiology Reports, 1 (2), 124-130. doi:10.1111/j.1758-2229.2009.00017.x

Ongena, M., Nihorimbere, V., Fickers, P., & Thonart, P. (2009). Influence of rhizosphere-specific parameters on surfactin production by Bacillus subtilis. Bulletin OILB/SROP, (43), 317-320.

Nihorimbere, V., Fickers, P., Thonart, P., & Ongena, M. (September 2008). Influence of rhizosphere-specific parameters on surfactin production by Bacillus subtilis [Paper presentation]. Molecular tools for understanding and improving biocontrol, Interlaken, Switzerland.

Nihorimbere, V., Fickers, P., Thonart, P., & Ongena, M. (August 2008). Ecological fitness of plant beneficial Bacillus subtilis strains in soil: influence of rhizosphere-specific parameters on surfactin synthesis [Poster presentation]. 9th International Congress of Plant Pathology, Turin, Italy.

Leclere, V., Bechet, M., Brans, A., Coutte, F., Chollet-Imbert, M., Fickers, P., Gancel, F., Guez, J. S., Joris, B., Ongena, M., & Jacques, P. (March 2008). Bacillus lipopeptides properties leading to versatile weapons for plant disease biocontrol [Paper presentation]. BACELL 2008, Oslo, Norway.

Fickers, P., Destain, J., & Thonart, P. (2008). Les lipases sont des hydrolases atypiques : principales caractéristiques et applications. Biotechnologie, Agronomie, Société et Environnement, 12 (2), 119-130.

Fickers, P., Leclere, V., Guez, J.-S., Bechet, M., Coucheney, F., Joris, B., & Jacques, P. (2008). Temperature dependence of mycosubtilin homologue production in Bacillus subtilis ATCC6633. Research in Microbiology, 159 (6), 449-57. doi:10.1016/j.resmic.2008.05.004

Jacques, P., Leclere, V., Bechet, M., Coutte, F., Chollet-Imbert, M., Dhulster, P., Fickers, P., Gancel, F., Lecouturier, D., & Guez, J. S. (2008). Genetic and bioprocess engineering applied to the overproduction of biosurfactant from Bacillus subtilis [Paper presentation]. 3rd International Congress on Bioprocesses in Food Industries, Hyderabad, India.

Nihorimbere, V., Fickers, P., Thonart, P., & Ongena, M. (2008). Ecological fitness of plant-beneficial Bacillus subtilis strains in soil: influence of rhizosphere specific parameters on surfactin synthesis. Journal of Plant Pathology, 90, 2.122.

Nihorimbere, V., Ongena, M., Fickers, P., & Thonart, P. (11 October 2007). Ecological fitness of plant beneficial Bacillus subtilis strains in soil: influence of rhizosphere-specific parameters on surfactin synthesis [Poster presentation]. Bioforum, Liège, Belgium.

Coutte, F., Bechet, M., Leclere, V., Fickers, P., Brans, A., Joris, B., Lecouturier, P., Dhulster, P., & Jacques, P. (2007). Influence of overexpression of mycosubtilin or surfactin synthetase on the phenotype of Bacillus subtilis strains [Poster presentation]. 14th International Conference on Bacilli, Pise, Italy.

Fickers, P., Brans, A., Barry, R., Joris, B., Bechet, M., Leclere, V., & Jacques, P. (2007). Engineering Bacillus subtilis ATCC6633 for the production of the lipopeptide mycosubtilin [Poster presentation]. 14th International Conference on Bacilli, Pise, Italy.

Leclere, V., Marti, R., Bechet, M., Fickers, P., & Jacques, P. (2007). Par leurs propriétés de surface, la mycosubtiline et la surfactine augmentent la colonisation par Bacillus subtilis [Poster presentation]. VIIème Congrès de la Société Française de Microbiologie, Nantes, France.

Fickers, P., Ongena, M., Destain, J., Weekers, F., & Thonart, P. (2006). Production and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica. Enzyme and Microbial Technology, 38 (6), 756-759. doi:10.1016/j.enzmictec.2005.08.005

Leclere, V., Marti, R., Bechet, M., Fickers, P., & Jacques, P. (2006). The lipopeptides mycosubtilin and surfactin enhance spreading of Bacillus subtilis strains by their surface-active properties. Archives of Microbiology, 186 (6), 475-83. doi:10.1007/s00203-006-0163-z

Fickers, P., Fudalej, F., Le Dall, M. T., Casaregola, S., Gaillardin, C., Thonart, P., & Nicaud, J. M. (March 2005). Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytiea. Fungal Genetics and Biology, 42 (3), 264-274. doi:10.1016/j.fgb.2004.12.003

Fickers, P., Nicaud, J. M., Destain, J., & Thonart, P. (March 2005). Involvement of hexokinase HXK1 in glucose catabolite repression of LIP2 encoding extracellular lipase in the yeast Yarrowia lipolytica. Current Microbiology, 50 (3), 133-137. doi:10.1007/s00284-004-4401-9

Fickers, P., Fudalej, F., Nicaud, J. M., Destain, J., & Thonart, P. (February 2005). Selection of new over-producing derivatives for the improvement of extracellular lipase production by the non-conventional yeast Yarrowia lipolytica. Journal of Biotechnology, 115 (4), 379-386. doi:10.1016/j.jbiotec.2004.09.014

Destain, J., Fickers, P., Weekers, F., Moreau, B., & Thonart, P. (2005). Utilization of methyloleate in production of microbial lipase. Applied Biochemistry and Biotechnology, 121 (Spring), 269-277. doi:10.1385/ABAB:121:1-3:0269

Fickers, P., Benetti, P., Waché, Y., Marty, A., Mauersberger, S., Smit, M., & Nicaud, J. (2005). Hydrophobic substrate utilization by the yeast Yarrowia lipolytica, and its potential applications. FEMS Yeast Research, (5), 527-543. doi:10.1016/j.femsyr.2004.09.004

Fickers, P., Destain, J., & Thonart, P. (2005). Methyl Oleate Modulates Lip2 Expression In The Lipolytic Yeast Yarrowia Lipolytica. Biotechnology Letters, 27 (22), 1751-1754. doi:10.1007/s10529-005-3547-2

Thonart, P., Destain, J., Moreau, B., Fickers, P., Weekers, F., Molitor, J. P., & Weiss, A. (10 November 2004). Procédé de production de lipase.

Destain, J., Moreau, B., Weekers, F., Fickers, P., & Thonart, P. (May 2004). Utilization of methyloleate in the production of Yarrowia lipolytica [Poster presentation]. 26th Symposium on Biotechnology for Fuels and Chemicals, Chattanooga, Tennessee, United States.

Fickers, P., Nicaud, J. M., Gaillardin, C., Destain, J., & Thonart, P. (2004). Carbon and nitrogen sources modulate lipase production in the yeast Yarrowia lipolytica. Journal of Applied Microbiology, 96 (4), 742-749. doi:10.1111/j.1365-2672.2004.02190.x

Fickers, P., Le Dall, M. T., Gaillardin, C., Thonart, P., & Nicaud, J. M. (December 2003). New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica. Journal of Microbiological Methods, 55 (3), 727-737. doi:10.1016/j.mimet.2003.07.003

Fickers, P., Nicaud, J. M., Destain, J., & Thonart, P. (December 2003). Overproduction of lipase by Yarrowia lipolytica mutants. Applied Microbiology and Biotechnology, 63 (2), 136-142. doi:10.1007/s00253-003-1342-3

Fickers, P., Le Dal, M., Gaillardin, C., Thonart, P., Destain, J., & Nicaud, J. M. (09 May 2003). A novel lox/Cre-mediated multiple gene knockout procedue in the yeast Yarrowia lipolytica [Poster presentation]. Bioforum, Liège, Belgium.

Fickers, P., Gaillardin, C., Thonart, P., & Nicaud, J. M. (April 2003). Etude phénotypique de la famille multigénique LIP codant pour des enzymes lipolytiques chez la levure Yarrowia lipolytica [Paper presentation]. Levure, Modèle et Outil VI, Genève, Switzerland.

Fickers, P., Nicaud, J. M., Destain, J., & Thonart, P. (July 2002). Lipase production in Yarrowia lipolytica overproducing mutants [Poster presentation]. Third Yarrowia lipolytica international meeting. Cell biology and biotechnology of a nonconventional yeast, Dresden, Germany.

Destain, J., Moreau, B., Fickers, P., & Thonart, P. (September 2000). Chemical characterization of Yarrowia lipolytica extracellular lipase [Poster presentation]. Lipid Lipase Interaction, Lund, Sweden.

Fickers, P., Destain, J., & Thonart, P. (September 2000). Study of lipase production by yeast Yarrowia lipolytica [Poster presentation]. Lipid Lipase Interaction, Lund, Sweden.

Fickers, P., Destain, J., & Thonart, P. (31 March 2000). Improvement of lipase production by the yeast Yarrowia lipolytica [Poster presentation]. Bioforum, Liège, Belgium.