Publications of Silvia Turchetto
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See detailCoordination between Transport and Local Translation in Neurons
Broix, Loïc ULiege; Turchetto, Silvia ULiege; Nguyen, Laurent ULiege

in Trends in Cell Biology (2021)

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See detailATP-citrate lyase promotes axonal transport across species.
Even, Aviel; Morelli, Giovanni ULiege; Turchetto, Silvia ULiege et al

in Nature Communications (2021), 12(1), 5878

Microtubule (MT)-based transport is an evolutionary conserved process finely tuned by posttranslational modifications. Among them, α-tubulin acetylation, primarily catalyzed by a vesicular pool of α ... [more ▼]

Microtubule (MT)-based transport is an evolutionary conserved process finely tuned by posttranslational modifications. Among them, α-tubulin acetylation, primarily catalyzed by a vesicular pool of α-tubulin N-acetyltransferase 1 (Atat1), promotes the recruitment and processivity of molecular motors along MT tracks. However, the mechanism that controls Atat1 activity remains poorly understood. Here, we show that ATP-citrate lyase (Acly) is enriched in vesicles and provide Acetyl-Coenzyme-A (Acetyl-CoA) to Atat1. In addition, we showed that Acly expression is reduced upon loss of Elongator activity, further connecting Elongator to Atat1 in a pathway regulating α-tubulin acetylation and MT-dependent transport in projection neurons, across species. Remarkably, comparable defects occur in fibroblasts from Familial Dysautonomia (FD) patients bearing an autosomal recessive mutation in the gene coding for the Elongator subunit ELP1. Our data may thus shine light on the pathophysiological mechanisms underlying FD. [less ▲]

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See detailATAT1-enriched vesicles promote microtubule acetylation via axonal transport
Even, Aviel; Morelli, Giovanni ULiege; Broix, Loic et al

Poster (2019, June 13)

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See detailATAT1-enriched vesicles promote microtubule acetylation via axonal transport.
Even, Aviel; Morelli, Giovanni ULiege; Broix, Loïc ULiege et al

in Science Advances (2019), 5(12), 2705

Microtubules are polymerized dimers of alpha- and beta-tubulin that underlie a broad range of cellular activities. Acetylation of alpha-tubulin by the acetyltransferase ATAT1 modulates microtubule ... [more ▼]

Microtubules are polymerized dimers of alpha- and beta-tubulin that underlie a broad range of cellular activities. Acetylation of alpha-tubulin by the acetyltransferase ATAT1 modulates microtubule dynamics and functions in neurons. However, it remains unclear how this enzyme acetylates microtubules over long distances in axons. Here, we show that loss of ATAT1 impairs axonal transport in neurons in vivo, and cell-free motility assays confirm a requirement of alpha-tubulin acetylation for proper bidirectional vesicular transport. Moreover, we demonstrate that the main cellular pool of ATAT1 is transported at the cytosolic side of neuronal vesicles that are moving along axons. Together, our data suggest that axonal transport of ATAT1-enriched vesicles is the predominant driver of alpha-tubulin acetylation in axons. [less ▲]

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