Publications of Justine Javaux
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See detailLy6Chi monocytes are key immunoregulators of gammaherpesvirus infection
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Conference (2020, December 18)

Detailed reference viewed: 27 (5 ULiège)
See detailLy6Chi monocytes are key immunoregulators of gammaherpesvirus infection
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Conference (2020, November 20)

Detailed reference viewed: 25 (5 ULiège)
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See detailA gammaherpesvirus licenses CD8 T cells to protect the host from pneumovirus-induced immunopathologies.
Dourcy, Mickael ULiege; Maquet, Céline ULiege; Dams, Lorène ULiege et al

in Mucosal Immunology (2020)

Human respiratory syncytial virus (RSV) is a pneumovirus that causes severe infections in infants worldwide. Despite intensive research, safe and effective vaccines against RSV have remained elusive. The ... [more ▼]

Human respiratory syncytial virus (RSV) is a pneumovirus that causes severe infections in infants worldwide. Despite intensive research, safe and effective vaccines against RSV have remained elusive. The main reason is that RSV infection of children previously immunized with formalin-inactivated-RSV vaccines has been associated with exacerbated pathology, a phenomenon called RSV vaccine-enhanced respiratory disease. In parallel, despite the high RSV prevalence, only a minor proportion of children develop severe diseases. Interestingly, variation in the immune responses against RSV or following RSV vaccination could be linked with differences of exposure to microbes during childhood. Gammaherpesviruses (γHVs), such as the Epstein–Barr virus, are persistent viruses that deeply influence the immune system of their host and could therefore affect the development of pneumovirus-induced immunopathologies for the long term. Here, we showed that a previous ɣHV infection protects against both pneumovirus vaccine-enhanced disease and pneumovirus primary infection and that CD8 T cells are essential for this protection. These observations shed a new light on the understanding of pneumovirus-induced diseases and open new perspectives for the development of vaccine strategies. [less ▲]

Detailed reference viewed: 134 (33 ULiège)
See detailHelminth infection is associated with the accumulation of lung interstitial macrophages and increased susceptibility to γ-herpesvirus infection in C57BL/6 mice
Rolot, Marion; Preure, Amira ULiege; Dougall, Annette et al

Scientific conference (2020, March 06)

Detailed reference viewed: 42 (9 ULiège)
See detailLy6Chi monocytes are key orchestrators of gammaherpesvirus lifecycle
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Conference (2019, December 19)

Detailed reference viewed: 24 (5 ULiège)
See detailHelminth infection is associated with the accumulation of lung interstitial macrophages and increased susceptibility to gammaherpesvirus infection in C57BL/6 mice
Rolot, Marion; Preure, Amira; Dougall, Annette et al

Conference (2019, December 02)

Parasitic helminths can condition innate cells such as lung macrophages for accelerated clearance in a secondary infection. However, it is unknown how helminth-induced macrophage changes in the lung ... [more ▼]

Parasitic helminths can condition innate cells such as lung macrophages for accelerated clearance in a secondary infection. However, it is unknown how helminth-induced macrophage changes in the lung affect the response to concurrent bystander infection with viruses. Here, we have examined the lung macrophage responses after Schistosoma mansoni egg-induced inflammation or Nippostrongylus brasiliensis infection and observed a severe accumulation of CD11b+ interstitial macrophages (IntMs) while siglec-F+ alveolar macrophages (AlvMs) were reduced. Interestingly, expansion of IntMs was more pronounced in C57BL/6 compared to BALB/c mice and we observed a strong upregulation of the M(IL-4) alternative activation marker YM1 in IntMs of C57BL/6, compared to BALB/c mice. We then examined the susceptibility of both mouse strains to murid gammaherpesvirus 4 (MuHV-4) infection and observed that helminth exposure rendered C57BL/6 mice highly susceptible to MuHV-4 acute infection whereas BALB/c mice controlled viral infection earlier as previously published by our group through the expansion of “virtual” memory CD8+ T cells (Tvm) (Rolot et al., 2018. Nat Commun. 2018 Oct 30;9(1):4516). We further confirmed the role of IL-4-induced Tvm in the CD8-mediated control of MuHV-4 in both C57BL/6 and BALB/c strains, suggesting that the increased early susceptibility of C57BL/6 mice to MuHV-4 does not affect the induction of effector CD8+ T cells. Finally, we observed increased proportions of MuHV-4-infected macrophages after 4 days post-infection when C57BL/6 mice were exposed to helminths, suggesting that helminth infection modifies the lung macrophage niche to become more permissive to MuHV-4 infection. [less ▲]

Detailed reference viewed: 57 (9 ULiège)
See detailLy6Chi monocytes are key orchestrators of gammaherpesvirus lifecycle
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Conference (2019, November 14)

Detailed reference viewed: 12 (1 ULiège)
See detailRole of monocytes in Murid gammaherpesvirus 4 lifecycle
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Conference (2019, November 08)

Detailed reference viewed: 18 (2 ULiège)
See detailLy6Chi monocytes are key orchestrators of gammaherpesvirus lifecycle
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Poster (2019, September 13)

Detailed reference viewed: 18 (2 ULiège)
See detailHelminth infection is associated with expansion of lung interstitial macrophages and increased susceptibility to gammaherpesvirus infection in C57BL/6 mice
Rolot, Marion; Preure, Amira; Dougall, Annette et al

Conference (2019, June 24)

Exposure to parasitic helminths can imprint the immune system to modulate bystander immune responses to viral infection. We have recently shown that IL-4 induced during helminth infection expand bystander ... [more ▼]

Exposure to parasitic helminths can imprint the immune system to modulate bystander immune responses to viral infection. We have recently shown that IL-4 induced during helminth infection expand bystander “virtual” memory CD8+ T cells (TVM) that results in enhanced control of murid gammaherpesvirus 4 (MuHV-4) lytic infection in the lung (Rolot et al., 2018. Nat Commun. 2018 Oct 30;9(1):4516). Whereas the frequency of TVM in secondary lymphoid organs in homeostasis is known to be higher in Balb/c compared to C57BL/6 mice, we observed here that IL-4c treatment or helminth exposure induced TVM expansion and enhanced CD8-mediated control of MuHV-4 in both strains. However, lung exposure to helminths in C57BL/6 mice resulted in severely increased viral loads after MuHV-4 infection. Such increased susceptibility to MuHV-4 infection was restricted to the strain C57BL/6 and was observed after induction of Schistosoma mansoni egg-induced lung inflammation and after Nippostrongylus brasiliensis infection. Following viral respiratory infection, MuHV-4 virions are first licensed by lung macrophages to infect alveolar epithelial cells, suggesting that macrophage responses could be affected by helminth-induced inflammation. Interestingly, helminth exposure induced a severe expansion of CD11b+ interstitial macrophages displaying M(IL-4) polarisation with increased Relm-α, PD-L2 and MHC-II expression. In addition, we observed increased proportions of MuHV-4-infected macrophages when C57BL/6 mice were exposed to helminths. Altogether, these results suggest that helminth infection affects the lung macrophages which become more permissive to MuHV-4 infection. [less ▲]

Detailed reference viewed: 37 (8 ULiège)
See detailFunction of monocytes in Murid herpesvirus 4 lifecycle
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Poster (2019, May 21)

Detailed reference viewed: 18 (2 ULiège)
See detailFunction of monocytes in Murid herpesvirus 4 lifecycle
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Poster (2019, February 21)

Detailed reference viewed: 33 (4 ULiège)
See detailLy6Chi monocytes are key orchestrators of gammaherpesvirus lifecycle
Maquet, Céline ULiege; Loos, Pauline ULiege; Javaux, Justine ULiege et al

Conference (2018, December 20)

Detailed reference viewed: 13 (2 ULiège)
See detailRNA-seq analysis of latently-infected CD8+ T lymphocytes during bovine malignant catarrhal fever reveals unconventional TCR cell activation
Myster, Françoise ULiege; Javaux, Justine ULiege; Van Campe, Willem et al

Conference (2018, December 20)

Alcelaphine herpesvirus 1 (AlHV-1) persists in wildebeest asymptomatically but induces malignant catarrhal fever (MCF) upon transmission to other ruminant species, including cattle. We have previously ... [more ▼]

Alcelaphine herpesvirus 1 (AlHV-1) persists in wildebeest asymptomatically but induces malignant catarrhal fever (MCF) upon transmission to other ruminant species, including cattle. We have previously shown that MCF is a lethal disease resembling a peripheral T cell lymphoma (PTCL) and induced after ORF73-dependent latency establishment in CD8+ T lymphocytes. However, how AlHV-1 infection leads to CD8+ T lymphocyte proliferation and activation remains largely unknown. In this study, we investigated viral and cellular transcriptomes of bovine CD8+ T lymphocytes during MCF. Three groups of four 4-month-old calves were mock-infected or infected intranasally with 105 PFU of the virulent C500 wildtype (WT) strain or non-pathogenic ORF73-deficient (73null) AlHV-1 strain. At day 45 after infection (mock and 73null groups) or at time of MCF development (WT group), CD8+ T lymphocytes were isolated from peripheral blood to high purity before RNA extraction. Illumina Truseq stranded mRNA libraries were obtained and subjected to NextSeq500 sequencing. Transcriptomes were analysed for both viral and cellular transcripts. We took advantage of the non-pathogenic 73null strain to differentiate anti-viral effector/memory responses in CD8+ T cells from transcriptomics changes due to latent infection of these cells during MCF. While no viral transcripts could be detected in CD8+ T cells of mock or 73null-infected animals, expression of viral genes of interest in MCF-developing calves was detected including AlHV-1-specific semaphorin-like A3, undefined A4, bZIP protein A6, interleukin 4-like protein A9.5 and undefined glycoprotein A10. When analysing differential cellular gene expression (P < 10−4; change in expression of over fourfold), we observed upregulation of 151 (WT vs Mock) and 72 (WT vs 73null) genes, including Gzma, SerpinB9, Moxd1, Tox2, IL-10, and Cxcr3; and downregulation of 205 (WT vs Mock) and 271 (WT vs 73null) genes, including αv or β3 integrins. Intriguingly, while gene-set enrichment for TCR engagement was observed in CD8+ T cells from MCF-developing calves, supporting T cell activation, we also observed strong downregulation of gene transcripts normally involved in canonical TCR activation such as Cbl, Tec family tyrosine kinases Rlk, Tec, and Itk, and Syk. These data suggest unconventional TCR triggering and provide unprecedented mechanistic insights on how AlHV-1 could induce a PTCL-like disease. Future work should further determine the actual pathway(s) involved and which viral gene(s) drive T cell dysregulation. [less ▲]

Detailed reference viewed: 51 (8 ULiège)