Publications of Mutien-Marie Garigliany
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See detailHumpback whales (Megaptera novaeangliae) breeding off Mozambique and Ecuador show geographic variation of persistent organic pollutants and isotopic niches
Remili, Anaïs; Gallego Reyes, Pedro ULiege; Pinzone, Marianna ULiege et al

in Environmental Pollution (2020), 267

Humpback whales (Megaptera novaeangliae) from the Southern Hemisphere carry information on persistent organic pollutants (POPs) from their feeding zones in Antarctica to their breeding grounds, making ... [more ▼]

Humpback whales (Megaptera novaeangliae) from the Southern Hemisphere carry information on persistent organic pollutants (POPs) from their feeding zones in Antarctica to their breeding grounds, making this species a sentinel of contaminants accumulation in the Southern Ocean. This study aimed to evaluate driving factors, namely feeding areas, trophic level, and sex, affecting POP concentrations in the blubber of humpback whales breeding off Mozambique and off Ecuador. Biopsies of free-ranging humpback whales including blubber and skin were collected in 2014 and 2015 from Ecuador (n = 59) and in 2017 from Mozambique (n = 89). In both populations, HCB was the major contaminant followed by DDTs > CHLs > PCBs > HCHs > PBDEs. POP concentrations were significantly higher in males compared to females. HCB, DDTs, HCHs and PBDEs were significantly different between whales from the Mozambique population and the Ecuador population. Sex and feeding habits were important driving factors accounting for POP concentrations in Ecuador whales. The whales from our study had some of the lowest POP concentrations measured for humpback whales in the world. These whales fed predominantly on krill as reflected from the low δ13C and δ15N values measured in the skin. However, the isotopic niches of whales from Mozambique and Ecuador did not overlap indicating that the two populations are feeding in different areas of the Southern Ocean. [less ▲]

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See detailSARS-COV-2 Natural Transmission from Human to Cat, Belgium, March 2020
Garigliany, Mutien-Marie ULiege; Van Laere, Anne-Sophie ULiege; Clercx, Cécile ULiege et al

in Emerging Infectious Diseases (2020), 26(12), 3069-3071

In March 2020, a severe respiratory syndrome developed in a cat, 1 week after its owner received positive test results for severe acute respiratory syndrome coronavirus 2. Viral RNA was detected in the ... [more ▼]

In March 2020, a severe respiratory syndrome developed in a cat, 1 week after its owner received positive test results for severe acute respiratory syndrome coronavirus 2. Viral RNA was detected in the cat’s nasopharyngeal swab samples and vomitus or feces; immunoglobulin against the virus was found in convalescent-phase serum. Human-to-cat transmission is suspected. [less ▲]

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See detailAspergillus qPCR testing on nasal swab: A useful tool for diagnosis and follow‐up of sinonasal aspergillosis in dogs?
Biénès, Tom ULiege; Fastrès, Aline ULiege; Vangrinsven, Emilie ULiege et al

in Journal of Veterinary Internal Medicine (2020, October 17), 34(6), 3058-3166

Polymerase chain reaction (PCR) testing either for Aspergillus.spp or for Aspergillus fumigatus is now available; however, the interest of such tests in the diagnosis of canine sinonasal aspergillosis ... [more ▼]

Polymerase chain reaction (PCR) testing either for Aspergillus.spp or for Aspergillus fumigatus is now available; however, the interest of such tests in the diagnosis of canine sinonasal aspergillosis (SNA) has not yet been assessed. The aim of this study was to evaluate the presence of fungal material using qPCR targeting Aspergillus.spp (PanAsp) and A. fumigatus (Aspfum) in samples obtained from nasal cavities of dogs with various nasal diseases and healthy dogs. In SNA dogs, Aspfum and PanAsp were positive in 13/20 and 14/20 dogs with a mean cycle threshold (Ct) of 30.6 [range 23,2 ‐ 33,3] and 28.3 [24,3 ‐ 34,5], respectively. The PanAsp was also positive in 3 non‐SNA dogs: one with cured SNA, one with LPR and one with nasal tumor, but at very low load (Ct>33). Results between both qPCR were highly correlated (r = 0.8, P < 0.01). For Aspfum and PanAsp, the sensitivity was 65% and 70% and the specificity was 100% and 94%, respectively. Aspfum qPCR test on deep blinded nasal swabs appears highly specific but only moderately sensitive to diagnose canine SNA. In some dogs fungal plaques are exclusively found in the frontal sinus and are probably not reached by blinded sampling. Since A. fumigatus is the most common etiological agent of canine SNA (96.7% of isolates), Aspfum testing appears appropriate; however, PanAsp testing is a non‐negligible tool to detect the small percentage of SNA cases related to other Aspergillus species. Results also show that healthy predisposed dogs do not seem to be carriers and confirm that A. fumigatus does not appear to have a major role in LPR. The negative results found in cured SNA dogs show a good correlation with clinical and rhinoscopic findings. In conclusion, Aspfum and/or PanAsp (qPCR testing) on deep nasal blinded swabs can be useful for the detection of SNA at diagnosis and after cure. [less ▲]

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See detailHémorragie pulmonaire aiguë associée à une herpèsvirose atypique
Loublier, Clémence ULiege; Cesarini Latorre, Carlota ULiege; Garigliany, Mutien-Marie ULiege et al

in Pratique Vétérinaire Équine (2020), 52, 207(207), 43-47

A 6-year-old halfbred gelding horse was referred for coughing, serious nasal discharge and epistaxis. Examinations revealed petechiae on the upper respiratory tract, acute pulmonary haemorrhage, slight ... [more ▼]

A 6-year-old halfbred gelding horse was referred for coughing, serious nasal discharge and epistaxis. Examinations revealed petechiae on the upper respiratory tract, acute pulmonary haemorrhage, slight haematuria and severe azotaemia. A diagnosis of equine herpesvirus type 1 infection with endotheliotropic strain D752 was confirmed by polymerase chain reaction (PCR) on whole blood and nasal swab. This atypical presentation is probably explained by the vascular tropism of the virus, which appears to be multisystemic, including respiratory and renal as seen in this case. [less ▲]

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See detailAnti-Schmallenberg virus activities of type I/III interferons-induced Mx1 GTPases from different mammalian species
Bayrou, Calixte ULiege; Van Laere, Anne-Sophie ULiege; Dam Van, Phai et al

Conference (2020, September 29)

• Objectives Type I/ III interferons provide powerful and universal innate intracellular defense mechanisms against viruses. Among the antiviral effectors induced, Mx proteins of some species appear as ... [more ▼]

• Objectives Type I/ III interferons provide powerful and universal innate intracellular defense mechanisms against viruses. Among the antiviral effectors induced, Mx proteins of some species appear as key components of antiviral defense. The most studied isoform, the human MxA protein, is known as the “Swiss army knife” of the antiviral response due to its ability to inhibit the cellular amplification of numerous viruses belonging to, at least, 12 different families. The bovine counterpart (the bovine Mx1 protein, BoMx1) has been shown to impair the amplification of 5 different viral family members. The Schmallenberg virus (SBV) belongs to the family Peribunyaviridae and was described for the first time in 2011. After having emerged in Northern Europe, it rapidly spread across the continent causing limited or no symptoms in adult ruminants but severe malformations in a small proportion of in utero infected fetuses. The objective of this study was to look for a cause of the ruminant sensitivity to the SBV infection in comparison to other mammal species. The question was: could the ruminant sensitivity be linked to a lack of antiviral Mx1 activity against the SBV? To answer this question, four different Mx1 isoforms originated from different mammals were studied in vitro. • Materials and methods The study was conducted in cell culture-based conditions. A lipid complex (Lipofectamine® 3000 Transfection reagent kit) transfection model was used to transiently express the Mx1 protein of four different species (the bovine, canine, equine and porcine isoforms) and compare their antiviral activities. After the transfected cells being infected with the SBV, the amount of Mx1 protein and nucleoprotein (NP) of the virus was measured using the fluorescent-activated cell sorting (FACS) technology. Human embryonic kidney cells (HEK-293T) were transfected with expression plasmids designed to express the different Mx1 isoforms tagged with the V5 epitope which was flanked on the N-terminal extremity of each Mx1 protein. The presence of the V5 epitope allowed an absolute standardization of the detection of the different V5-Mx1 isoforms. After a 24h incubation post-transfection, the cells were infected with the SBV (SBV-BH80/11-4) and cell fixation was realized after 5 hours of viral amplification. The immunolabelling was achieved using, on the one hand, a phycoerythrin (PE) conjugated monoclonal antibody targeting the V5 epitope allowing the Mx1 protein detection, and, on the other hand, a primary mouse monoclonal antibody targeting the SBV nucleoprotein associated with a goat anti-mouse IgG polyclonal antibody conjugated to the fluorescein isothiocyanate (FITC) fluorochrome. The results expressed the mean intensity of 3 independent assay (one assay comprising 3 wells for each condition and 100,000 cells analyzed per well). The statistical analysis was performed by analysis of variance (ANOVA). • Results All the tested Mx1 protein isoforms showed an antiviral effect. Indeed, in a same well, the percentage of NP expressing cells was significantly lower in the V5-Mx1 positive population than in the V5-Mx1 negative population. Among the different Mx isoforms, the antiviral effect of the canine Mx1 protein was significantly less pronounced. In the conditions of this experiment a clear dose-dependent effect was seen: an increase in the Mx1-associated fluorescence intensity was correlated with a reduction of the number of NP positive cells until an almost complete absence of the NP signal in the cells with the highest Mx fluorescence values. • Conclusions The bovine species sensitivity to the SBV infection is not linked to a lack of antiviral activity of the bovine Mx1 protein against the virus. The experiment corroborates the results showing that the Mx1 proteins have been selected during evolution to efficiently inhibits the amplification of a large diversity of viruses including the Peribunyaviridae family. The dose-dependent effect we herein described could reflect an unknown specific mechanism of the SBV inhibition by the Mx1 protein. [less ▲]

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See detailIn Vitro and In Vivo Models to Study the Zoonotic Mosquito-Borne Usutu Virus.
Benzarti, Emna ULiege; Garigliany, Mutien-Marie ULiege

in Viruses (2020), 12(10),

Usutu virus (USUV), a mosquito-borne zoonotic flavivirus discovered in South Africa in 1959, has spread to many European countries over the last 20 years. The virus is currently a major concern for animal ... [more ▼]

Usutu virus (USUV), a mosquito-borne zoonotic flavivirus discovered in South Africa in 1959, has spread to many European countries over the last 20 years. The virus is currently a major concern for animal health due to its expanding host range and the growing number of avian mass mortality events. Although human infections with USUV are often asymptomatic, they are occasionally accompanied by neurological complications reminiscent of those due to West Nile virus (another flavivirus closely related to USUV). Whilst USUV actually appears less threatening than some other emergent arboviruses, the lessons learned from Chikungunya, Dengue, and Zika viruses during the past few years should not be ignored. Further, it would not be surprising if, with time, USUV disperses further eastwards towards Asia and possibly westwards to the Americas, which may result in more pathogenic USUV strains to humans and/or animals. These observations, inviting the scientific community to be more vigilant about the spread and genetic evolution of USUV, have prompted the use of experimental systems to understand USUV pathogenesis and to boost the development of vaccines and antivirals. This review is the first to provide comprehensive coverage of existing in vitro and in vivo models for USUV infection and to discuss their contribution in advancing data concerning this neurotropic virus. We believe that this paper is a helpful tool for scientists to identify gaps in the knowledge about USUV and to design their future experiments to study the virus. [less ▲]

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See detailAnalysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction
Fastrès, Aline ULiege; Canonne-Guibert, Morgane ULiege; Taminiau, Bernard ULiege et al

in BMC Veterinary Research (2020), 51(46), 1-12

Infection with Bordetella bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM ... [more ▼]

Infection with Bordetella bronchiseptica (Bb), a pathogen involved in canine infectious respiratory disease complex, can be confirmed using culture or qPCR. Studies about the canine lung microbiota (LM) are recent, sparse, and only one paper has been published in canine lung infection. In this study, we aimed to compare the LM between Bb infected and healthy dogs, and to correlate sequencing with culture and qPCR results. Twenty Bb infected dogs diagnosed either by qPCR and/or culture and 4 healthy dogs were included. qPCR for Mycoplasma cynos (Mc) were also available in 18 diseased and all healthy dogs. Sequencing results, obtained from bronchoalveolar lavage fluid after DNA extraction, PCR targeting the V1–V3 region of the 16S rDNA and sequencing, showed the presence of Bbin all diseased dogs, about half being co‑infected with Mc. In diseased compared with healthy dogs, the β‑diversity changed (P=0.0024); bacterial richness and α‑diversity were lower (P=0.012 and 0.0061), and bacterial load higher (P= 0.004). Bb qPCR classes and culture results correlated with the abundance of Bb (r = 0.71, P < 0.001 and r = 0.70, P= 0.0022). Mc qPCR classes also correlated with the abundance of Mc (r = 0.73, P < 0.001). Bb infection induced lung dysbiosis, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, compared with healthy dogs. Sequencing results highly correlate with qPCR and culture results showing that sequencing can be reliable to identify microorganisms involved in lung infectious diseases. [less ▲]

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See detailNew Insights into the Susceptibility of Immunocompetent Mice to Usutu Virus.
Benzarti, Emna ULiege; Sarlet, Michaël ULiege; Franssen, Mathieu ULiege et al

in Viruses (2020), 12(2),

Usutu virus (USUV) is a mosquito-borne flavivirus that shares many similarities with the closely related West Nile virus (WNV) in terms of ecology and clinical manifestations. Initially distributed in ... [more ▼]

Usutu virus (USUV) is a mosquito-borne flavivirus that shares many similarities with the closely related West Nile virus (WNV) in terms of ecology and clinical manifestations. Initially distributed in Africa, USUV emerged in Italy in 1996 and managed to co-circulate with WNV in many European countries in a similar mosquito-bird life cycle. The rapid geographic spread of USUV, the seasonal mass mortalities it causes in the European avifauna, and the increasing number of infections with neurological disease both in healthy and immunocompromised humans has stimulated interest in infection studies to delineate USUV pathogenesis. Here, we assessed the pathogenicity of two USUV isolates from a recent Belgian outbreak in immunocompetent mice. The intradermal injection of USUV gave rise to disorientation and paraplegia and was associated with neuronal death in the brain and spinal cord in a single mouse. Intranasal inoculation of USUV could also establish the infection; viral RNA was detected in the brain 15 days post-infection. Overall, this pilot study probes the suitability of this murine model for the study of USUV neuroinvasiveness and the possibility of direct transmission in mammals. [less ▲]

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See detailExperimental Usutu Virus Infection in Domestic Canaries Serinus canaria.
Benzarti, Emna ULiege; Rivas Troncoso, José Felipe Antonio ULiege; Sarlet, Michaël ULiege et al

in Viruses (2020), 12(2),

Usutu virus (USUV) is a neurotropic flavivirus closely related to West Nile virus (WNV). Its enzootic cycle mainly involves mosquitoes and birds. Human infection can occur with occasional, but sometimes ... [more ▼]

Usutu virus (USUV) is a neurotropic flavivirus closely related to West Nile virus (WNV). Its enzootic cycle mainly involves mosquitoes and birds. Human infection can occur with occasional, but sometimes severe, neurological complications. Since its emergence and spread in Europe over the last two decades, USUV has been linked to significant avian outbreaks, especially among Passeriformes, including European blackbirds (Turdus merula). Strikingly, no in vivo avian model exists so far to study this arbovirus. The domestic canary (Serinus canaria) is a passerine, which is considered as a highly susceptible model of infection by WNV. Here, we experimentally challenged domestic canaries with two different doses of USUV. All inoculated birds presented detectable amounts of viral RNA in the blood and RNA shedding via feathers and droppings during the early stages of the infection, as determined by RT-qPCR. Mortality occurred in both infected groups (1/5 and 2/5, respectively) and was not necessarily correlated to a pure neurological disease. Subsequent analyses of samples from dead birds showed histopathological changes and virus tropism mimicking those reported in naturally infected birds. A robust seroconversion followed the infection in almost all the surviving canaries. Altogether, these results demonstrate that domestic canaries constitute an interesting experimental model for the study of USUV pathogenesis and transmission. [less ▲]

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See detailNatural transmission of SARS-CoV-2 infection and associated disease from human to pet cat (Felis silvestris catus), Belgium, March 2020
Garigliany, Mutien-Marie ULiege; Van Laere, Anne-Sophie ULiege; Clercx, Cécile ULiege et al

in Emerging Infectious Diseases (2020)

In early March 2020, a household cat suddenly developed a severe respiratory syndrome one week after its owner tested positive for the SARS-CoV-2 virus. The viral RNA was detected in the nasopharyngeal ... [more ▼]

In early March 2020, a household cat suddenly developed a severe respiratory syndrome one week after its owner tested positive for the SARS-CoV-2 virus. The viral RNA was detected in the nasopharyngeal swabs, vomit or feces and convalescent serum collected 3 weeks after the onset of symptoms contained anti-SARS-CoV-2 immunoglobulins. [less ▲]

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See detailUsutu Virus Infection of Embryonated Chicken Eggs and a Chicken Embryo-Derived Primary Cell Line.
Benzarti, Emna ULiege; Rivas Troncoso, José Felipe Antonio ULiege; Sarlet, Michaël ULiege et al

in Viruses (2020), 12(5),

Usutu virus (USUV) is a mosquito-borne flavivirus, closely related to the West Nile virus (WNV). Similar to WNV, USUV may cause infections in humans, with occasional, but sometimes severe, neurological ... [more ▼]

Usutu virus (USUV) is a mosquito-borne flavivirus, closely related to the West Nile virus (WNV). Similar to WNV, USUV may cause infections in humans, with occasional, but sometimes severe, neurological complications. Further, USUV can be highly pathogenic in wild and captive birds and its circulation in Europe has given rise to substantial avian death. Adequate study models of this virus are still lacking but are critically needed to understand its pathogenesis and virulence spectrum. The chicken embryo is a low-cost, easy-to-manipulate and ethically acceptable model that closely reflects mammalian fetal development and allows immune response investigations, drug screening, and high-throughput virus production for vaccine development. While former studies suggested that this model was refractory to USUV infection, we unexpectedly found that high doses of four phylogenetically distinct USUV strains caused embryonic lethality. By employing immunohistochemistry and quantitative reverse transcriptase-polymerase chain reaction, we demonstrated that USUV was widely distributed in embryonic tissues, including the brain, retina, and feather follicles. We then successfully developed a primary cell line from the chorioallantoic membrane that was permissive to the virus without the need for viral adaptation. We believe the future use of these models would foster a significant understanding of USUV-induced neuropathogenesis and immune response and allow the future development of drugs and vaccines against USUV. [less ▲]

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See detailUsutu Virus Epizootic in Belgium in 2017 and 2018: Evidence of Virus Endemization and Ongoing Introduction Events.
Benzarti, Emna ULiege; Sarlet, Michaël ULiege; Franssen, Mathieu ULiege et al

in Vector Borne and Zoonotic Diseases (2020)

Wildlife surveillance allowed the monitoring of the zoonotic mosquito-borne Usutu virus (USUV) in birds and bats (Pipistrellus pipistrellus) in southern Belgium in 2017 and 2018. USUV-RNA was detected in ... [more ▼]

Wildlife surveillance allowed the monitoring of the zoonotic mosquito-borne Usutu virus (USUV) in birds and bats (Pipistrellus pipistrellus) in southern Belgium in 2017 and 2018. USUV-RNA was detected in 69 birds (of 253) from 15 species, among which 7 species had not previously been reported to be susceptible to the infection. Similarly, 2 bats (of 10) were detected positive by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). USUV-associated lesions were mainly found in Eurasian Blackbirds (Turdus merula), in which USUV antigens were demonstrated by immunohistochemistry in the brain, heart, liver, kidney, intestine, and lung. Partial nonstructural protein 5 gene-based phylogenetic analysis showed several identical or closely related strains from 2016, 2017, and 2018 clustering together within Europe 3 or Africa 3 lineages. Further, one USUV strain detected in a common chaffinch (Fringilla coelebs) manifested a close genetic relationship with the European 1 strains circulating in Hungary and Austria. Our data provide evidence of USUV endemization in southern Belgium in local birds and bats, extension of the host range of the virus and ongoing virus introduction from abroad, likely by migratory birds. Our results highlight the need for vigilance in the forthcoming years toward new virus-associated outbreaks in birds and possible human infections in Belgium. [less ▲]

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See detailPutative Role of Arthropod Vectors in African Swine Fever Virus Transmission in Relation to Their Bio-Ecological Properties
Bonnet, Sarah I.; Bouhsira, Emilie; De Regge, Nick et al

in Viruses (2020), 12

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See detailAn expert opinion assessment of blood-feeding arthropods based on their capacity to transmit African swine fever virus in Metropolitan France.
Saegerman, Claude ULiege; Bonnet, Sarah; Bouhsira, Emilie et al

in Transboundary and emerging diseases (2020)

To deal with the limited literature data on the vectorial capacity of blood-feeding arthropods (BFAs) and their role in the transmission of African swine fever virus (ASFV) in Metropolitan France, a ... [more ▼]

To deal with the limited literature data on the vectorial capacity of blood-feeding arthropods (BFAs) and their role in the transmission of African swine fever virus (ASFV) in Metropolitan France, a dedicated working group of the French Agency for Food, Environmental and Occupational Health & Safety performed an expert knowledge elicitation. In total, 15 different BFAs were selected as potential vectors by the ad hoc working group involved. Ten criteria were considered to define the vectorial capacity: vectorial competence, current abundance, expected temporal abundance, spatial distribution, longevity, biting rate, active dispersal capacity, trophic preferences for Suidae, probability of contact with domestic pigs and probability of contact with wild boar. Fourteen experts participated to the elicitation. For each BFA, experts proposed a score (between 0 and 3) for each of the above criteria with an index of uncertainty (between 1 and 4). Overall, all experts gave a weight for all criteria (by distributing 100 marbles). A global weighted sum of score per BFA was calculated permitting to rank the different BFAs in decreasing order. Finally, a regression tree analysis was used to group those BFAs with comparable likelihood to play a role in ASF transmission. Out of the ten considered criteria, the experts indicated vectorial competence, abundance and biting rate as the most important criteria. In the context of Metropolitan France, the stable fly (Stomoxys calcitrans) was ranked as the most probable BFA to be a vector of ASFV, followed by lice (Haematopinus suis), mosquitoes (Aedes, Culex and Anopheles), Culicoides and Tabanidea. Since scientific knowledge on their vectorial competence for ASF is scarce and associated uncertainty on expert elicitation moderate to high, more studies are however requested to investigate the potential vector role of these BFAs could have in ASFV spread, starting with Stomoxys calcitrans. [less ▲]

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See detailMechanical transmission of African swine fever virus by Stomoxys calcitrans: Insights from a mechanistic model
Vergne, T.; Andraud, M.; Bonnet, S. et al

in Transboundary and Emerging Diseases (2020)

African swine fever (ASF) represents a global threat with huge economic consequences for the swine industry. Even though direct contact is likely to be the main transmission route from infected to ... [more ▼]

African swine fever (ASF) represents a global threat with huge economic consequences for the swine industry. Even though direct contact is likely to be the main transmission route from infected to susceptible hosts, recent epidemiological investigations have raised questions regarding the role of haematophagous arthropods, in particular the stable fly (Stomoxys calcitrans). In this study, we developed a mechanistic vector-borne transmission model for ASF virus (ASFV) within an outdoor domestic pig farm in order to assess the relative contribution of stable flies to the spread of the virus. The model was fitted to the ecology of the vector, its blood-feeding behaviour and pig-to-pig transmission dynamic. Model outputs suggested that in a context of low abundance (<5 flies per pig), stable flies would play a minor role in the spread of ASFV, as they are expected to be responsible for around 10% of transmission events. However, with abundances of 20 and 50 stable flies per pig, the vector-borne transmission would likely be responsible for almost 30% and 50% of transmission events, respectively. In these situations, time to reach a pig mortality of 10% would be reduced by around 26% and 40%, respectively. The sensitivity analysis emphasized that the expected relative contribution of stable flies was strongly dependent on the volume of blood they regurgitated and the infectious dose for pigs. This study identified crucial knowledge gaps that need to be filled in order to assess more precisely the potential contribution of stable flies to the spread of ASFV, including a quantitative description of the populations of haematophagous arthropods that could be found in pig farms, a better understanding of blood-feeding behaviours of stable flies and the quantification of the probability that stable flies partially fed with infectious blood transmit the virus to a susceptible pig during a subsequent blood-feeding attempt. © 2020 Wiley-VCH GmbH [less ▲]

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See detailPathogens discovery from imported meat seized at Brussels Airport (2017-2018).
Garigliany, Mutien-Marie ULiege

Conference (2019, December 03)

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See detailAnalysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction.
Fastrès, Aline ULiege; Canonne-Guibert, Morgane ULiege; Taminiau, Bernard ULiege et al

Poster (2019, November 08)

Infection with Bordetella bronchiseptica (Bb) can be confirmed using bacterial culture or quantitative polymerase chain reaction (qPCR). The lung microbiota (LM), which has been described in healthy ... [more ▼]

Infection with Bordetella bronchiseptica (Bb) can be confirmed using bacterial culture or quantitative polymerase chain reaction (qPCR). The lung microbiota (LM), which has been described in healthy experimental dogs, has not yet been studied in dogs with lower respiratory infection. In the present study we aimed to analyze the LM in dogs with Bb infection compared with healthy dogs, and to correlate the 16S rDNA amplicon sequencing results with culture and qPCR results. Twenty dogs with a diagnosis of Bb infection obtained either by qPCR and/or culture and 4 healthy dogs were included. 16S rDNA sequences were obtained from naïve bronchoalveolar lavage fluid (BALF) after DNA extraction, PCR targeting the V1-V3 region of the 16S rDNA and sequencing. Sequencing results showed the presence of Bb in all diseased dogs. About half of the dogs were co-infected, the majority with M. cynos. In diseased dogs, a shift in the β-diversity of the LM was observed (P=0.002); the richness and the α-diversity were significantly lower (P=0.012 and 0.006) and the bacterial load higher (P=0.004) compared with healthy dogs. Bb qPCR level and culture results were positively correlated with the relative abundance of Bb species after sequencing (r= 0.56, P=0.028 and r=0.70, P=0.002). Bb induced a major dysbiosis of the LM, characterized by high bacterial load, low richness and diversity and increased abundance of Bb, in comparison with healthy dogs. Results of the LM analysis highly correlate with results obtained by qPCR and culture and show that results of LM can be reliable for identification of potentially causal bacterial microorganism involved in lung infectious diseases. [less ▲]

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See detailNewly emerged African swine fever virus strain Belgium/Etalle/wb/2018: Complete genomic sequence and comparative analysis with reference p72 genotype II strains
Gilliaux, Gautier ULiege; Garigliany, Mutien-Marie ULiege; Licoppe, Alain ULiege et al

in Transboundary and Emerging Diseases (2019), 66(6), 2566-2591

In a new example of pathogens hopscotching the globe, African swine fever virus hit north‐western Europe's wildlife in summer 2018, marking a further spread of a disease that had invaded Central and ... [more ▼]

In a new example of pathogens hopscotching the globe, African swine fever virus hit north‐western Europe's wildlife in summer 2018, marking a further spread of a disease that had invaded Central and Eastern Europe recently. The complete genomic sequence of the Belgium/Etalle/wb/2018 virus is reported, with the hope it will provide a valuable tool for tracing geographical spread and biologic evolution of the virus. [less ▲]

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