Publications of Jérôme Ponthier
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See detailCase Report: Suppression of Harem Stallion Behavior and Fertility Following Anti-Gonadotropin-Releasing Hormone Vaccination of a Captive Wild Przewalski's Horse (Equus ferus przewalskii)
Ponthier, Jérôme ULiege; Rigaux, Goulven; Parrilla Hernandez, Sonia ULiege et al

in Frontiers in Veterinary Science (2020), 7

This report describes an option to modulate the testicular function of wild horses and field methods to assess it. Non-surgical castration of a captive wild Przewalski’s stallion with anti-gonadotropin ... [more ▼]

This report describes an option to modulate the testicular function of wild horses and field methods to assess it. Non-surgical castration of a captive wild Przewalski’s stallion with anti-gonadotropin-releasing hormone (GnRH) immunization was performed by sub-cutaneous injection of two doses of 450 μg (3 ml) of GnRH conjugated to diphtheria toxin, further repeated every 6 months. Semen quality was assessed after collection by electro-ejaculation under general anesthesia. Endocrine and behavioral consequences were studied during a 2-year follow-up period. The procedure of electro-ejaculation was safe and effective to collect spermatozoa. Motility was low but was improved by a significant dilution of sample (1v/4v−1v/5v) after collection. Immuno-neutering resulted in a decrease of the total spermatozoa number and motility 1 month after primary vaccination. However, infertility could not yet be guaranteed. Six months post-vaccination, serum testosterone concentrations had decreased and the treated stallion had lost his harem stallion role. Moreover, at the same time, the total spermatozoa number was near zero with no motile spermatozoa, and offspring was no longer observed. As a conclusion, electro-ejaculation under general anesthesia is suitable on wild horses to obtain spermatozoa that should be washed or largely diluted before use for artificial insemination (AI) programs. Anti-GnRH immuno-neutering protocol led to a dramatic decrease of spermatozoa number, motility, and testosterone production. This also induced deep changes in the social structure of the band. Such technique could be considered as an alternative to surgical castration in wild horses. [less ▲]

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See detailEndoscopy Guided Photoablation of Endometrial Cysts using a 980 nm Laser with a Contact Fiber in Mares
Ponthier, Jérôme ULiege; de la Rebière de Pouyade, Geoffroy ULiege; Salciccia, Alexandra ULiege et al

in Journal of Visualized Experiments (2020)

In mares, endometrial cysts are associated with endometriosis and can cause maternal recognition failure or compromise and delay pregnancy diagnoses. Historical treatments were invasive and had adverse ... [more ▼]

In mares, endometrial cysts are associated with endometriosis and can cause maternal recognition failure or compromise and delay pregnancy diagnoses. Historical treatments were invasive and had adverse effects on the endometrium. Hysteroscopically guided laser therapy is easy and effective for endometrial cysts resection, with no deleterious effects for the endometrium. A 110 cm long and 1.0 cm wide endoscope is sterilely introduced in the uterus through the open cervix of an estrous mare after vulvar cleaning. The uterus is slowly infused with less than 1 L of physiologic solution and the laser fiber is inserted in the biopsy canal of the endoscope. Cysts are then cauterized with the 980 nm diode laser with a contact fiber set at 20‒2 5W in continuous mode. Each cyst is punctured until complete voiding of the cyst and shrinking of the cyst wall around the fiber. Uterine lavages with sterile saline solution are performed directly after the surgery and for one or two days as non-inflammatory fluid can be observed. This procedure is easy and quickly performed, with no obvious deleterious effects. Cysts resection makes ultrasound pregnancy diagnosis easier and, in some cases, could restore proper embryo migration in the uterine horns between day 6.5 and 17. However, this treatment does not improve the underlying histological lesions related to endometriosis. These considerations should be clearly expressed to the breeder before this procedure. [less ▲]

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See detailProceedings of the 7th FARAH-Day
Bayrou, Calixte ULiege; Cesarini Latorre, Carlota ULiege; Delguste, Catherine ULiege et al

Book published by Presses de la Faculté de Médecine vétérinaire de l'Université de Liège (2020)

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See detailInadequate mare-foal bonding restored by adoption of her own foal - a case report
Parrilla Hernandez, Sonia ULiege; Deleuze, Stefan ULiege; Wimmer-Scherr, Christina ULiege et al

Poster (2019, September 21)

Difficulties in mare-foal bonding are mostly the consequence of an abnormal maternal behaviour. Despite current therapies usually based on restrain and sedation of the mare as well as adapted management ... [more ▼]

Difficulties in mare-foal bonding are mostly the consequence of an abnormal maternal behaviour. Despite current therapies usually based on restrain and sedation of the mare as well as adapted management of each situation, the mare-foal bonding is not always created. However, adoption of foster foals and establishment of the maternal behaviour is often successful. Procedures of adoption mimic the transient increase of oxytocin during delivery and its effects by cervical and vaginal stimulation (Fergusson reflex), or by pharmacological simulation of the high concentrations of prostaglandins observed during parturition. Even if the mechanism is not yet well understood in mares, both hormones seem to have a direct effect on the brain control of maternal behaviour. A primiparous 7 years old mare was presented at the Equine Clinic showing aggressive behaviour towards her 4 days filly especially when the foal attempted to suckle. No evident cause of pain at suckling was found. A hormonal adoption procedure of her own foal was implemented after several attempts to restore the mare-foal bonding by restrain and sedation of the mare. After 3h of fasting and isolation of the foal, the mare received an im injection of 750µg of cloprostenol. The foal was carefully presented to the mare when the secondary effects of the prostaglandins were visible. The mare accepted the foal and allowed suckling rapidly. During the next 24 hours under supervision, the mare showed a very normal maternal behaviour. After 3 days of normal bonding, mare and foal were discharged from the clinic. Adoption of filial foals could be a valuable option in the treatment to establish or restore the normal bonding between a mare and her own foal. [less ▲]

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See detailOptimizing drone raising and marking techniques in Belgium: a report.
Egyptien, Sophie ULiege; Brutinel, Flore ULiege; Ponthier, Jérôme ULiege et al

Conference (2019, September)

Research on drone semen freezing requires very large numbers of drones, which can be very challenging. No established technique to supply these large numbers has been clearly described. We report our ... [more ▼]

Research on drone semen freezing requires very large numbers of drones, which can be very challenging. No established technique to supply these large numbers has been clearly described. We report our attempts to reach sufficient numbers of drones while maintaining a viable balance in the breeding system under Belgian beekeeping conditions. Controlling the age of drones can also be an issue. We report marking drones every 24h for up to 16days using one different color POSCA® Marker per day. Colored drones were well tolerated by the workers. Drones frames were introduced in a strong colony in a Dadant 10 frames hive for the queen to lay eggs. 24h before first hatching the frames were placed in a Dadant 6 frames hive and 2 different caging techniques were tested. Technique 1: Males were kept on a frame in a cage that was opened for daily marking. We observed that the cage quickly got overcrowded and drones tended to escape or get crushed and killed during the manipulations. The technique was then slightly modified and drones were individually collected from the frame, marked and then placed on a caged workers frame. This, however, was associated with too a high number of drones flying away during the manipulation and was abandoned. Technique 2: A maximum of 3 frames of males, 1 of workers and 1 of food were transferred into the body of a Dadant 6 frames hive placed on top of an empty super with a queen excluder between them. Date of birth was assessed by daily marking of the emerged drones. Drones were at first well tolerated but after one month they were chased out of the hive and killed by the workers. Drones raised in the small hive were smaller than drones that escaped and were raised by the nearby colonies. They also had diarrhea and almost no semen could consequently be collected. We conclude that raising large numbers of drones in surrogate hives is suboptimal. An alternative where drones are raised in their home hive and kept in small groups in small cages should be investigated. [less ▲]

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See detailImpact of oral clomiphene citrate administration on prostatic volume and plasmatic testosterone concentrations in male beagles
Egyptien, Sophie ULiege; Beaufays, France ULiege; Brutinel, Flore ULiege et al

in Reproduction in Domestic Animals (2019, September), 54(3), 63-64

Clomiphene citrate (CC) is a synthetic non-steroidal agonist/antagonist oestrogenic compound with reported agonistic properties on dogs’ male reproductive system. This protocol compared the impact of a 0 ... [more ▼]

Clomiphene citrate (CC) is a synthetic non-steroidal agonist/antagonist oestrogenic compound with reported agonistic properties on dogs’ male reproductive system. This protocol compared the impact of a 0.5mg/kg/day treatment of CC on plasmatic testosterone concentrations and prostatic volume (PV) on 2 groups of 5 young (<1.5years) healthy versus old (>8years) dogs with benign prostatic hyperplasia (BPH). Each dog was its own control and parameters were evaluated weekly over 2 periods of 8 weeks: 1 week pre-treatment, 3 weeks of treatment (placebo or CC) and 4 weeks post-treatment. Prostatic measures made by transabdominal ultrasonography were fed in the formula: (1/2.6 (lxLxh)) + 1.8) to calculate the PV (cm3). Plasmatic testosterone concentration (ng/ml) was measured by radioimmunoassay on samples obtained at the end of the morning. A linear mixed model was built and differences of least squares means were used to analyse the results and test the effects of treatment, week, age and their interactions. CC dogs have significantly lower PV values (10.36) than control dogs (12.91; p=0.01). CC old dogs have significantly lower PV values (15.05) than control old dogs (18.31; p=0.03). PV significantly (p=0.01) decreases in CC dogs from week 1 to 5 and then re-increases from week 6 to 8. CC dogs have significantly lower testosterone levels (0.7543) than controls (1.17; p=0.01). There is a significant interaction between the week and the treatment as shown by a transient decrease in testosterone similar to that of the PV. We conclude that CC decreases testosterone production and prostatic volume, which opens doors for BPH treatment. [less ▲]

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See detailMaladies infectieuses en reproduction et néonatalogie équine: état des lieux en Belgique
Ponthier, Jérôme ULiege; Barrandeguy, Maria Edith

Conference (2019, May 14)

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See detailLes effets de l'exercice sur la fertilité chez l'étalon de sport
Ponthier, Jérôme ULiege

in Nouveau Praticien Vétérinaire Equine (2019), Hors-série(12), 147-151

Les résultats sportifs d’un étalon conditionnent les demandes pour des saillies ou des doses d’insémination. Malheureusement, l’entraînement et la compétition limite la disponibilité de l’étalon de sport ... [more ▼]

Les résultats sportifs d’un étalon conditionnent les demandes pour des saillies ou des doses d’insémination. Malheureusement, l’entraînement et la compétition limite la disponibilité de l’étalon de sport, diminuant sa prolificité au moment du démarrage de sa carrière reproductrice. Cette revue propose de revoir les effets du sport et de la température sur la physiologie et l’endocrinologie du testicule et ses conséquences sur la fertilité. [less ▲]

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See detailProceedings of the 6th FARAH-Day
Bayrou, Calixte ULiege; Delguste, Catherine ULiege; Douny, Caroline ULiege et al

Book published by Presses de la Faculté de Médecine vétérinaire de l’Université de Liège (2019)

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See detailOn-field Electro-ejaculation of Wild Prezwalski Horses: Results and Perspectives
Rigaux, Goulven; Parrilla Hernandez, Sonia ULiege; Egyptien, Sophie ULiege et al

in Journal of Equine Veterinary Science (2018), 66

hus, semen collected from wild horses by electro-ejaculation performed under anaesthesia could be shipped after large dilution to the lab and eventually used for AI programs, if CASA results show ... [more ▼]

hus, semen collected from wild horses by electro-ejaculation performed under anaesthesia could be shipped after large dilution to the lab and eventually used for AI programs, if CASA results show sufficient motility. [less ▲]

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See detailClinical case: Management of twins in mares
Ponthier, Jérôme ULiege

Scientific conference (2018, July 05)

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See detailHot topics session: Contraception in the equine
Ponthier, Jérôme ULiege

Scientific conference (2018, July 05)

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See detailEmbryo transfer in mares: Why? When? How?
Ponthier, Jérôme ULiege

Scientific conference (2018, July 04)

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See detailProceedings of the 5th FARAH-Day
Bayrou, Calixte ULiege; Cabaraux, Jean-François ULiege; Delguste, Catherine ULiege et al

Book published by Presse de la Faculté de Médecine Vétérinaire de l'Université de Liège (2018)

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See detailA case of uterine hematoma in a mare after an endometrial biopsy
Parrilla Hernandez, Sonia ULiege; Reignier, Fabrice; Barrière, Philippe et al

Poster (2017, August 26)

Endometrial biopsy is an important, safe and painless procedure to assess equine uterine health. While minor bleeding at the site of sampling is a common condition, uterine hematoma is a rare complication ... [more ▼]

Endometrial biopsy is an important, safe and painless procedure to assess equine uterine health. While minor bleeding at the site of sampling is a common condition, uterine hematoma is a rare complication. An endometrial biopsy was routinely performed on an unsedated 17 year-old welsh pony mare in estrus during a research protocol. From the next day on and during the following 7 days, a large amount of sanguineous fluid was observed in the uterine cavity during the daily ultrasound examination performed according with the protocol. No fever or any other systemic symptoms were noticed. Two weeks later, the ultrasound examination revealed an organised hyperechogenic mass compatible with a hematoma within the endometrial lumen. The mare was regularly controlled during the rest of the breeding season and no abnormalities in her cyclicity were observed. The size of the hematoma only began to decrease from the 3rd month after the biopsy onward and it disappeared completely 2 months later. This was the only complication following a biopsy in this study protocol that included a total of 70 biopsies on 49 mares and the very first case the authors got to see while biopsies have been common practice in their clinical and research activities for years. To our knowledge, this is the first time that the appearance of this condition is documented. The hematoma took 5 months to disappear at the ultrasound examination. During this time the mare could not be bred. Therefore, while endometrial biopsy should still be regarded as a safe procedure for the diagnosis of infertility in mares, the potential risk of a hematoma with its consequences for the breeding season has to be considered. [less ▲]

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See detailAborder la fertilité chez l'étalon et la jument
Ponthier, Jérôme ULiege; Deleuze, Stefan ULiege

Conference given outside the academic context (2017)

Cette conférence se propose de définir quels sont les facteurs de fertilité chez la jument comme chez l'étalon et d'étudier les pathologies ou mauvaises pratiques pouvant mener à de l'infertilité dans l ... [more ▼]

Cette conférence se propose de définir quels sont les facteurs de fertilité chez la jument comme chez l'étalon et d'étudier les pathologies ou mauvaises pratiques pouvant mener à de l'infertilité dans l'espèce équine. [less ▲]

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See detailProceedings of the 4th FARAH-Day
Bayrou, Calixte ULiege; Cabaraux, Jean-François ULiege; Delguste, Catherine ULiege et al

Book published by Presses de la Faculté de Médecine vétérinaire de l’Université de Liège (2017)

Detailed reference viewed: 61 (18 ULiège)