Publications of Tatiana Art
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See detailAcylcarnitine profile in Alaskan sled dogs during submaximal multiday exercise points out metabolic flexibility and liver role in energy metabolism.
Tosi, Irène ULiege; Art, Tatiana ULiege; BOEMER, François ULiege et al

in PLoS ONE (2021), 16(8), 0256009

Alaskan sled dogs develop a particular metabolic strategy during multiday submaximal exercise, allowing them to switch from intra-muscular to extra-muscular energy substrates thus postponing fatigue ... [more ▼]

Alaskan sled dogs develop a particular metabolic strategy during multiday submaximal exercise, allowing them to switch from intra-muscular to extra-muscular energy substrates thus postponing fatigue. Specifically, a progressively increasing stimulus for hepatic glycogenolysis and gluconeogenesis provides glucose for both fueling exercise and replenishing the depleted muscle glycogen. Moreover, recent studies have shown that with continuation of exercise sled dogs increase their insulin-sensitivity and their capacity to transport and oxidize glucose and carbohydrates rather than oxidizing fatty acids. Carnitine and acylcarnitines (AC) play an essential role as metabolic regulators in both fat and glucose metabolism; they serve as biomarkers in different species in both physiologic and pathologic conditions. We assessed the effect of multiday exercise in conditioned sled dogs on plasma short (SC), medium (MC) and long (LC) chain AC by tandem mass spectrometry (MS/MS). Our results show chain-specific modification of AC profiles during the exercise challenge: LCACs maintained a steady increase throughout exercise, some SCACs increased during the last phase of exercise and acetylcarnitine (C2) initially increased before decreasing during the later phase of exercise. We speculated that SCACs kinetics could reflect an increased protein catabolism and C2 pattern could reflect its hepatic uptake for energy-generating purposes to sustain gluconeogenesis. LCACs may be exported by muscle to avoid their accumulation to preserve glucose oxidation and insulin-sensitivity or they could be distributed by liver as energy substrates. These findings, although representing a "snapshot" of blood as a crossing point between different organs, shed further light on sled dogs metabolism that is liver-centric and more carbohydrate-dependent than fat-dependent and during prolonged submaximal exercise. [less ▲]

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See detailIn Vitro Assays for the Assessment of Impaired Mitochondrial Bioenergetics in Equine Atypical Myopathy.
Kruse, Caroline ULiege; Stern, David ULiege; Mouithys-Mickalad, Ange ULiege et al

in Life (Basel, Switzerland) (2021), 11(7),

Equine atypical myopathy is a seasonal intoxication of grazing equids. In Europe, this poisoning is associated with the ingestion of toxins contained in the seeds and seedlings of the sycamore maple (Acer ... [more ▼]

Equine atypical myopathy is a seasonal intoxication of grazing equids. In Europe, this poisoning is associated with the ingestion of toxins contained in the seeds and seedlings of the sycamore maple (Acer pseudoplatanus). The toxins involved in atypical myopathy are known to inhibit ß-oxidation of fatty acids and induce a general decrease in mitochondrial respiration, as determined by high-resolution respirometry applied to muscle samples taken from cases of atypical myopathy. The severe impairment of mitochondrial bioenergetics induced by the toxins may explain the high rate of mortality observed: about 74% of horses with atypical myopathy die, most within the first two days of signs of poisoning. The mechanism of toxicity is not completely elucidated yet. To improve our understanding of the pathological process and to assess therapeutic candidates, we designed in vitro assays using equine skeletal myoblasts cultured from muscle biopsies and subjected to toxins involved in atypical myopathy. We established that equine primary myoblasts do respond to one of the toxins incriminated in the disease. [less ▲]

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See detailEvaluation of the accuracy of a commercial heart rate monitoring system to detect RR-waves interval in Warmblood horses
Frippiat, Thibault ULiege; van Beckhoven, Cees; Moyse, Evelyne ULiege et al

Scientific conference (2020, November 20)

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See detailIn vitro bactericidal activity of nebulized silver on equine common respiratory bacteria
Paindaveine, Charlotte ULiege; Frippiat, Thibault ULiege; Mainil, Jacques ULiege et al

in Journal of Veterinary Internal Medicine (2019), 33

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See detailMeasured and predicted oxygen uptake in healthy adults
Art, Tatiana ULiege; Detilleux, Johann ULiege; Balligand, Marc ULiege et al

Poster (2019)

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See detailUse of methacholine bronchoprovocation test for the diagnosis of asymptomatic severe equine asthma
Frippiat, Thibault ULiege; Frellstedt, Linda; Tosi, Irène ULiege et al

Poster (2017)

Reasons for performing study: A group of experts in the field of equine respiratory disorders has recently proposed to rename Inflammatory Airway Disease and Recurrent Airway Obstruction as mild to ... [more ▼]

Reasons for performing study: A group of experts in the field of equine respiratory disorders has recently proposed to rename Inflammatory Airway Disease and Recurrent Airway Obstruction as mild to moderate and severe equine asthma (SEA) respectively, in reference of the human asthma. One of the characteristics of asthma, in equine and human patients, is the absence of clinical signs during clinical remission. Methacholine bronchoprovocation test (BPT), which is a standard diagnostic test for detection of asymptomatic human asthma, has been used in some research protocols in horses but no study has examined its potential value for clinical diagnostic. Objectives: To assess whether the methacholine bronchoprovocation test is an applicable and repeatable clinical test to distinguish control horses and SEA horses in clinical remission. Methods: Twelve horses (6 with SEA history and 6 controls). Seven of them (3 SEA and 4 control horses) underwent BPT twice at a 24-hour-interval to determine the repeatability of the test. Then all horses were tested before and after a 7-days period in a stall environment. Simultaneously, usual ancillary pulmonary examinations were performed. Results: The BPT had a good feasibility and a significant repeatability. Before the stay in stalls, SEA and controls horses could not be differentiated by the BPT. After a 7-days period of straw and hay exposure, the bronchial hyperresponsiveness was increased in both SEA and control horses, while the other clinical or functional parameters were not significantly affected. The 7-days period in stalls resulted in a significant difference in bronchoreactivity between SEA and control horses. Conclusions: Methacholine BPT does not permit to differentiate control and asymptomatic SEA horses in clinical remission, unless the horses have been kept in stalls. Potential relevance: These results suggest that the mechanisms underlying the bronchial hyperresponsiveness in asthma differ between human and equine patients. Nevertheless, the BPT may be used to detect asymptomatic severe asthmatic horses after a light environmental challenge. Further researches are needed to assess the clinical interest of BPT for subclinical mild-moderate equine asthma patients. [less ▲]

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See detailClinical outcome after surgical correction of cleft palate by laryngeal tie-forward in 2 horses
Tosi, Irène ULiege; Arevalo Rodriguez, José Manuel ULiege; Salciccia, Alexandra ULiege et al

Poster (2017)

Introduction: Cleft palate is a rare congenital defect in horses. Its description in literature is generally limited to cases of young foals referred for milk regurgitation from nostrils, coughing and ... [more ▼]

Introduction: Cleft palate is a rare congenital defect in horses. Its description in literature is generally limited to cases of young foals referred for milk regurgitation from nostrils, coughing and dysphagia, with a guarded to poor prognosis if not treated. Surgical intervention consists mainly in palatoplasty that is considered a salvage procedure and complications are frequent. Only few cases of cleft palate in adult horses are described in literature. Laryngeal tie-forward, a surgical technique generally used to treat dorsal displacement of the soft palate, has been described only in one 4-year-old pony with cleft palate but clinical improvement after surgery was partial and temporary. Objectives: To describe laryngeal tie-forward as a valid option for the treatment of cleft palate in two adult horses not suitable for palatoplasty. Methods: Clinical records of two 8 y.o. jumping horses performing respectively at a low and intermediate intensity of exercise and competition. Both horses were referred for respiratory noise, exercise intolerance, cough and alimentary nasal discharge; they were diagnosed with a moderate soft palate defect and surgically treated by laryngeal tie-forward. A clinical examination and control endoscopy were realized at 4 months for one horse and at 3 years after surgery for the other. Results: In both horses postoperative endoscopy showed a visible reduction of the gap between soft palate and the larynx so that the epiglottis was covering the soft palate defect. One horse was examined 4 months after surgery, a residual respiratory noise during exercise was still present but lighter than prior to intervention, its performances had remarkably improved. The second horse was controlled 3 years after surgery, some cough was still observed but limited to the onset of exercise, performances were satisfying and the horse was working at the same intensity level. Globally, in both horses, initial symptoms had disappeared or were significantly reduced after surgery and owners were satisfied. Reduction of the soft palate defect was still effective, even several months after surgery. Conclusions: The description of these two clinical cases shows that some horses can grow up to adult age with moderate defects of the soft palate; they are likely to show clinical signs of variable severity. In such cases laryngeal tie-forward should be considered as an option if the defect is too large or asymmetrical to be corrected with palatoplasty. [less ▲]

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See detailLearning to look - Purpose and design of an awareness-raising online course in veterinary sciences
Tasnier, Sophie ULiege; Busoni, Valeria ULiege; Hanzen, Christian ULiege et al

in Prilla, M; Ullmann, T; Kravcik, M (Eds.) et al Proceedings of the 5th Workshop on Awareness and Reflection in Technology-Enhanced Learning (2015, September 15)

This paper reports on a work in progress: an online self-instruction course created to stimulate students’ awareness processes when dealing with pictures. Using non-clinical material, the “Learning to ... [more ▼]

This paper reports on a work in progress: an online self-instruction course created to stimulate students’ awareness processes when dealing with pictures. Using non-clinical material, the “Learning to Look” course was de-signed as a preliminary training to the observation of histological sections, radi-ological graphs, and other specialized visual material. Following a presentation of the project, salient results of a feedback questionnaire completed by 382 stu-dents about their experience of the course are provided. [less ▲]

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See detailThe Innate Immune Response of Equine Bronchial Epithelial Cells is Altered by Training
Frellstedt, Linda ULiege; Gosset, Philippe; Kervoaze, Gwenola et al

in Veterinary Research (2015), 46(3), 1-12

Respiratory diseases, including inflammatory airway disease (IAD), viral and bacterial infections, are common problems in exercising horses. The airway epithelium constitutes a major physical barrier ... [more ▼]

Respiratory diseases, including inflammatory airway disease (IAD), viral and bacterial infections, are common problems in exercising horses. The airway epithelium constitutes a major physical barrier against airborne infections and plays an essential role in the lung innate immune response mainly through toll-like receptor (TLR) activation. The aim of this study was to develop a model for the culture of equine bronchial epithelial cells (EBEC) in vitro and to explore EBEC innate immune responses in trained horses. Bronchial epithelial biopsies were taken from 6 adult horses during lower airway endoscopy. EBEC were grown in vitro by an explant method. The innate immune response of EBEC was evaluated in vitro by treatment with TLR ligands. TLR3 is the most strongly expressed TLR at the mRNA level in EBEC and stimulation of EBEC with Poly(I:C), an analog of viral dsRNA, triggers a strong secretion of IFN-β, TNF-α, IL-6 and CXCL8. We further evaluated the EBEC innate immune response in horses that underwent a 4-month-training program. While training had no effect on TLR mRNA expression in EBEC as well as in bronchial biopsies, it increased the production of IFN-β after stimulation with a TLR3 ligand and decreased the secretion of TNF-α and IL-6 after stimulation with a TLR2 and TLR3 ligand. These findings may be implicated in the increased risk for viral and bacterial infections observed in sport horses. Altogether, we report a successful model for the culture of EBEC that can be applied to the investigation of pathophysiologic conditions in longitudinal studies. [less ▲]

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See detailAltered mitochondrial oxidative phosphorylation capacity in horses suffering from polysaccharide storage myopathy
Tosi, Irène ULiege; Art, Tatiana ULiege; Cassart, Dominique ULiege et al

in Proceedings of the Third FMV Scientific Meeting (2013, October 11)

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See detailAltered mitochondrial oxidative phosphorylation capacity in horses suffering from polysaccharide storage myopathy
Tosi, Irène ULiege; Art, Tatiana ULiege; Cassart, Dominique ULiege et al

Poster (2013)

Introduction: Exertional rhabdomyolyses are a common cause of exercise intolerance in the equine athlete, and Polysaccharide Storage Myopathy (PSSM) is a widely described muscular pathology. It is ... [more ▼]

Introduction: Exertional rhabdomyolyses are a common cause of exercise intolerance in the equine athlete, and Polysaccharide Storage Myopathy (PSSM) is a widely described muscular pathology. It is characterized by an accumulation of abnormal glycogen in myofibers due to a genetic defect in the skeletal muscle glycogen synthase (GYS1) enzyme. We hypothesized that the energetic production through the oxidative phosphorylation (OXPHOS) in muscular mitochondria might be impaired in type-1 PSSM-affected horses. Material and Methods: Eight horses with a history of exertional rhabdomyolysis were tested for the GYS1 mutation. Muscle biopsies were collected and used for histological analysis and high resolution respirometry (HRR). HRR values from 3 groups of horses (PSSM-positive horses, horses with a history of myopathy but negative to PSSM and healthy controls) were compared using a linear mixed model to take into account repeated (2-3 times) measurements made for each horse. Results: In four horses histology revealed an accumulation of abnormal glycogen in myofibers. A severe depression of the maximal OXPHOS capacity was observed by HRR in all horses with exertional rhabodmyolysis, with lower values in PSSM-positive cases. Conclusions: Our study shows a severe decreased OXPHOS capacity in PSSM-affected horses. PSSM is considered primarily a defect in glycogen synthesis but altered OXPHOS might play a central role in its pathogenesis. [less ▲]

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See detailExercise modifies the innate immune response in equine bronchial epithelial cells
Frellstedt, Linda ULiege; Gosset, Philippe; Pirottin, Dimitri ULiege et al

in Proceedings of the 3rd Scientific Meeting of the Faculty of Veterinary Medicine (University of Liege - Belgium) (2013)

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See detailAnalgésie préemptive du porcelet avant la castration: comparaison de l'effet de l'acide tolfénamique et du méloxicam sur la douleur et le stress
Wavreille, José; Danard, Maylis ULiege; Servais, Vincent et al

in 42èmes Journées de la Recherche Porcine (2012)

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See detailLe microdamier d'expression: Un outil performant dans la compréhension de la réponse immunitaire à un stress causé par l'exercice chez le cheval revue de littérature
Schoeneker, Julie; Art, Tatiana ULiege; Pirottin, Dimitri ULiege et al

in Annales de Médecine Vétérinaire (2011), 154

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans ... [more ▼]

Malgré de multiples études, les interrelations entre l’exercice, le stress et la réponse immune sont peu définies. Or, la compréhension de ces interrelations pourrait jouer un rôle très important dans l’amélioration de la santé et des résultats sportifs des athlètes. En effet, l’exercice peut être reconnu comme un stress. Il induit des modifications de l’équilibre homéostatique qui peuvent à leur tour altérer la réponse immunitaire de l’hôte et donc sa susceptibilité aux maladies. L’adrénaline est la molécule essentielle de tout processus de stress. La technologie des microdamiers, outil majeur d’investigation transcriptomique, permet l’étude de l’expression génique de l’ensemble du génome. Son utilisation devrait donc permettre de mieux caractériser et définir les interrelations entre l’exercice, le stress et la réponse immune. Cette revue recense les interrelations connues entre la réponse immune à un stress adrénergique d’une part et la réponse immune à l’exercice d’autre part. Elle considère en outre la contribution potentielle des microdamiers à une meilleure compréhension des effets d’un stress, et plus particulièrement celui lié à l’exercice, sur l’immunité. [less ▲]

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See detailEpithelial expression of mRNA and protein for IL-6, IL-10 and TNF-alpha in endobronchial biopsies in horses with recurrent airway obstruction.
Riihimäki, Miia; Raine, Amanda; Pourazar, Jamshid et al

in BMC Veterinary Research (2008), 4

BACKGROUND: The aim of this study was to evaluate the contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of cytokines of innate immunity IL-6, IL-10 and ... [more ▼]

BACKGROUND: The aim of this study was to evaluate the contribution of bronchial epithelium to airway inflammation, with focus on mRNA and protein expression of cytokines of innate immunity IL-6, IL-10 and TNF-alpha, in horses with Recurrent Airway Obstruction (RAO) during exacerbation and in remission. RESULTS: Despite marked clinical and physiologic alterations between exacerbation and after remission in the RAO horses no differences were detected in either cytokine mRNA or protein levels. Moreover, the expression of investigated cytokines in RAO horses on pasture did not differ from controls.In comparing real-time PCR analysis to results of immunohistochemistry only IL-10 mRNA and protein levels in RAO horses on pasture were significantly correlated (rs = 0.893, p = 0.007). Curiously, in controls examined on pasture the TNF-alpha protein level was positively correlated to IL-10 mRNA expression (rs = 0.967, p = 0.007) and negatively correlated to IL-6 mRNA expression (rs = -0.971, p = 0.001). CONCLUSION: Given the complementary relationship of assessing cytokines directly by immunohistochemistry, or indirectly by PCR to mRNA, the lack of significant changes in either mRNA or protein levels of IL-6, IL-10 or TNF-alpha mRNA in RAO horses in exacerbation suggests that these particular cytokines in bronchial tissue may not play a substantive role in the active inflammation of this disease. To support this contention further studies examining time dependency of expression of IL-6, IL-10 or TNF-alpha are needed, as is expansion of the range of cytokines to include other key regulators of airway inflammation. [less ▲]

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See detailEvaluation de la condition sportive du cheval de complet sur le terrain
Van Erck, Emmanuelle ULiege; de Moffaerts, Brieuc; Art, Tatiana ULiege et al

in Proceedings du Congrès Annuel A.V.E.F. (2005)

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See detailEffets fonctionnels respiratoires induits par l'inhalation de glycopyrrolate chez le cheval poussif en crise
Art, Tatiana ULiege; de Moffarts, Brieuc; Van Erck, Emmanuelle ULiege et al

in Annales de Médecine Vétérinaire (2003), 147

Bronchoconstriction is one of the most constant clinical sign observed in heaves-affected horses in crisis. Cholinergic pathways are involved in the occurrence of this obstruction. Consequently ... [more ▼]

Bronchoconstriction is one of the most constant clinical sign observed in heaves-affected horses in crisis. Cholinergic pathways are involved in the occurrence of this obstruction. Consequently anticholinergic drugs are potentially able to improve the pulmonary function of horses in acute crisis. Among these, atropine and ipratropium bromide have been previously experimented and their beneficial effects reported. In the present work, glycopyrrolate, another synthetic anticholinergic molecule has been tested for bronchodilator efficiency in heaves-affected horses. An acute exacerbation of the illness was obtained by a natural challenge with hay and straw in 7 heaves-affected horses. The first day, the reversibility of the bronchospasm of these horses was controlled by intravenous administration of atropine. Then, pulmonary function tests including mechanics of breathing, ie measurement of pleural pressure changes and respiratory airflows, and arterial blond gases analysis, were performed before as well as 15 and 60 minutes alter inhalation of 1500 µg of glycopyrrolate (Robinul®). The crisis induced a dramatic increase of total pulmonary resistance and maximal pleural pressure changes as well as a decrease in dynamic lung compliance. These changes were significantly reversed 15 and 60 minutes after inhalation of glycopyrrolate. We did not observe any adverse effects similar to those classically observed with atropine. These results showed that glycopyrrolate is a very efficient bronchodilator that may be used by the practitioner to treat a heaves-affected horses in crisis [less ▲]

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