Publications and communications of Rosalie SACHELI

Penoy, N.* , Delma, K. L.* , Homkar, N., Karim Sakira, A., Egrek, S., Sacheli, R., Sacre, P.-Y., Grignard, B., Hayette, M.-P., Somé, T. I., Semdé, R., Evrard, B., & Piel, G. (15 February 2024). Development and optimization of a one step process for the production and sterilization of liposomes using supercritical CO2. International Journal of Pharmaceutics, 651, 123769. doi:10.1016/j.ijpharm.2024.123769

Sacheli, R., Knapp, J., PHOLIEN, C., EGREK, S., Léonard, P., Giot, J.-B., Delaere, B., BLETARD, N., Gofflot, S., Nollevaux, M.-C., Meunier, P., Lovinfosse, P., Pirotte, B., Truyens, C., Millon, L., Detry, O., & Hayette, M.-P. (20 November 2023). Genetic diversity of Echinococcus multilocularis specimens isolated from Belgian patients with alveolar echinococcosis using EmsB microsatellites analysis. Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases, 116, 105531. doi:10.1016/j.meegid.2023.105531

Sacheli, R., Philippe, C., Meex, C., MZOUGUI, S., Melin, P., & Hayette, M.-P. (17 October 2023). Occurrence of Vibrio spp. in Selected Recreational Water Bodies in Belgium during 2021 Bathing Season. International Journal of Environmental Research and Public Health, 20 (20), 6932. doi:10.3390/ijerph20206932

Bive, B. Z., Sacheli, R., Mudogo, C. N., Zakayi, P. K., Bontems, S., Lelo, G. M., & Hayette, M.-P. (25 August 2023). Correlation of antifungal susceptibility and sequence types within Cryptococcus neoformans VNI from HIV patients, and ERG11 gene polymorphism. Journal de Mycologie Médicale, 33 (4), 101428. doi:10.1016/j.mycmed.2023.101428

Egrek, S., Sacheli, R., Beaumaikers, E., Toussaint, F., Giot, J.-B., Léonard, P., Detry, O., Truyens, C., & Hayette, M.-P. (17 April 2023). Evaluation of a commercial IgG monotest assay: a new automated chemiluminescent immunoassay for the serodiagnosis of cystic echinococcosis [Poster presentation]. 31rd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID).

Detry, O., Bihain, C., Sacheli, R., EGREK, S., BLETARD, N., Meunier, P., Lovinfosse, P., Delwaide, J., Botembe Nicole, Larranaga E, Truyens C, Delaere B, Pirotte B, Giot, J.-B., Léonard, P., & Hayette, M.-P. (March 2023). Alveolar echinococcosis is increasing in Southern Belgium: a report of the Belgian National Reference Laboratory for Echinococcosis (BNRLE) and clinical experience of ECHINO- Liege. Acta Gastro-Enterologica Belgica, 86 (1), 25.

Delma, K. L., Penoy, N., Sakira, A. K., EGREK, S., Sacheli, R., Grignard, B., Hayette, M.-P., Issa Somé, T., Evrard, B., Semdé, R., & Piel, G. (11 January 2023). Use of supercritical CO2 for the sterilization of liposomes: Study of the influence of sterilization conditions on the chemical and physical stability of phospholipids and liposomes. European Journal of Pharmaceutics and Biopharmaceutics, 183, 112 - 118. doi:10.1016/j.ejpb.2023.01.002

Sacheli, R., & Hayette, M.-P. (2023). Rapport d'activité du centre national de référence pour les mycoses 2020-2021.

Egrek, S., Sacheli, R., Giot, J.-B., Truyens, C., Léonard, P., Van Esbroeck, M., Depypere, M., Miendje Deyi, V. Y., Montesinos Hernandez, M. I., Boelens, J., Van Acker, J., Oris, E., Litzroth, A., Detry, O., & Hayette, M.-P. (November 2022). Belgian laboratory surveillance of human alveolar and cystic echinococcosis in 2021 [Paper presentation]. Belgian Society of Parasitology & Protistology, 10th November 2022, Leuven, Belgium.

Zono, B., Sacheli, R., Nani-Tuma, H. S., Mavanga, A., Mwambi, J. A., Etondo, M., Kasumba, D. M., Moutschen, M., Lelo, G. M., & Hayette, M.-P. (20 September 2022). P461 Cryptococcosis screening and isolates characterization in asymptomatic people living with HIV in Kinshasa, DRC [Poster presentation]. ISHAM 2022 Congress. doi:10.1093/mmy/myac072.p461

Zono, B.* , SACHELI, R.* , Situakibanza Nani-Tuma, H.* , Kabututu Zakayi, P.* , Ka, A.* , Mbula Mambimbi, M.* , Muendele, G.* , BOREUX, R.* , Landu, N.* , Nzanzu Mudogo, C.* , M'Buze, P.-R.* , MOUTSCHEN, M.* , Meyer, W.* , Mvumbi Lelo, G.* , & HAYETTE, M.-P.*. (19 May 2022). Clinical epidemiology and high genetic diversity amongst Cryptococcus spp. isolates infecting people living with HIV in Kinshasa, Democratic Republic of Congo. PLoS ONE, 17 (5), 0267842. doi:10.1371/journal.pone.0267842

Zono, B., SACHELI, R., Kabututu, P., BONTEMS, S., Mvumbi, G., & HAYETTE, M.-P. (01 April 2022). Antifungal susceptibility testing of Cryptococcus strains from Kinshasa (DRC), intronic substitution of the ERG11 gene in one of the fluconazole resistant strains [Paper presentation]. Symposium BSHAM-SFMM, Gand, Belgium.

Sacheli, R. (31 March 2022). Easy and rapid screening method to detect the resistance to terbinafine in dermatophytes [Paper presentation]. 2022 BSHAM – SFMM Joint Symposium, Ghent, Belgium.

Ndiaye, M., SACHELI, R., Diongue, K., ADJETEY BAHUN, A., DARFOUF, R., Cheickh Seck, M., Badiane, A. S., Diallo, M. A., Dieng, T., HAYETTE, M.-P., & Ndiaye, D. (2022). Evaluation of the multiplex real time PCR DermaGenius assay for the detection of dermatophytes in hair samples from Senegal. Journal of Fungi. doi:10.3390/jof8010011

Philippe, C.* , SACHELI, R.* , MELIN, P., & HAYETTE, M.-P. (2022). National cartography of water points for the presence of Vibrio spp. in Belgium [Poster presentation]. 32nd ECCMID European congress of clinical microbiology and infectious diseases, Lisbon, Portugal.

Zono, B., MOUTSCHEN, M., Situakibanza, H., SACHELI, R., Kabututu, P., Mvumbi, G., & HAYETTE, M.-P. (10 December 2021). Comparaison des caractéristiques cliniques et biologiques des PVVIH présentant une méningite à Cryptococcus neoformans versus C. curvatus/C. laurentii [Paper presentation]. Journées scientifiques PNLS/RDC, Kinshasa, Congo - Kinshasa.

Zono, B., SACHELI, R., Situakibanza, H., Kabututu, P., MOUTSCHEN, M., Mvumbi, G., & HAYETTE, M.-P. (10 December 2021). Épidémiologie clinique et caractérisation moléculaire d’isolats de Cryptococcus spp. infectant les PVVIH à Kinshasa, RDC [Paper presentation]. Journées scientifiques PNLS, Kinshasa, Congo - Kinshasa.

Zono, B., MOUTSCHEN, M., SITUAKIBANZA, H., SACHELI, R., MUENDELE, G., KABUTUTU, P., BIAKABUSWA, A., LANDU, N., MVUMBI, G., & Hayette, M.-P. (15 November 2021). Comparison of clinical and biological characteristics of HIV infected patients presenting Cryptococcus neorformans versus C. curvatus /C. laurentii meningitis. BMC Infectious Diseases, 21 (1), 1-10. doi:10.1186/s12879-021-06849-3

SACHELI, R., & HAYETTE, M.-P. (2021). Antifungal Resistance in Dermatophytes: Genetic considerations, Clinical Presentations and Alternative Therapies. Journal of Fungi. doi:10.3390/jof7110983

Zono, B., SACHELI, R., Situakibanza, H., Kabututu, P., Mbula, M., Muendele, G., BOREUX, R., Landu, N., Mudogo, C., M'buze, P.-R., Moutschen, M., Mvumbi, G., & Hayette, M.-P. (28 October 2021). Molecular Analysis of Cryptococcus spp. Reveals Species Diversity and Multilocus Sequence Typing Heterogeneity among People Living with HIV in Kinshasa. Journal of Fungi, 7 (916), 42-43. doi:10.3390/jof7110916

Sacheli, R., Arendrup Maiken, Chowdhary Anuradha, Coppieters, W., Naomé, A., Baurain, D., Hanikenne, M., & Hayette, M.-P. (October 2021). Development of a bioinformatic tool for the treatment of WGS data for dermatophytes typing and characterization: Focus on squalene epoxidase mutations and terbinafine resistance [Poster presentation]. 10th Trends in Medical Mycology (TIMM), Aberdeen, United Kingdom.

Sacheli, R., Knapp Jenny, Miévis, O., Giot, J.-B., Léonard, P., Delaere Bénédicte, BLETARD, N., Detry, O., Millon Laurence, & Hayette, M.-P. (09 July 2021). Molecular typing of Belgian Echinococcus multilocularis specimens from alveolar echinococcosis human lesions using EmsB microsatellites analysis [Poster presentation]. 31st European Congress of Clinical Microbiology & Infectious Diseases (ECCMID).

Sacheli, R., Cuypers, L., Seidel, L., DARFOUF, R., Adjetey, C., Lagrou, K., & Hayette, M.-P. (2021). Correction to: Epidemiology of Dermatophytes in Belgium: A 5 Years’ Survey (Mycopathologia, (2021), 186, 3, (399-409), 10.1007/s11046-021-00542-4). Mycopathologia, 186 (6), 897. doi:10.1007/s11046-021-00590-w

SACHELI, R., Henri, A.-S., SEIDEL, L., ERNST, M., DARFOUF, R., ADJETEY BAHUN, A., SCHYNS, M., MARECHAL, L., MEEX, C., ARRESE ESTRADA, J., & HAYETTE, M.-P. (2020). Evaluation of the new Id-Fungi plates from Conidia for MALDI-TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples. Mycoses. doi:10.1111/myc.13156

Diop, C. S., DESCY, J., MEEX, C., SACHELI, R., & HAYETTE, M.-P. (April 2020). Saccharomyces cerevisiae fungaemia: a 10-year review in the CHU of Liege (Belgium) [Poster presentation]. 30th ECCMID.

MEEX, C., Douillez, S., Kinet-Poleur, A., SACHELI, R., & MELIN, P. (April 2020). Characterization of Group B Streptococci (GBS) colonizing pregnant women in Belgium, 2018: antimicrobial susceptibility profile and distributions of capsular-types, pili-types and sequence-types [Poster presentation]. 30th ECCMID, Paris, France.

SACHELI, R., Harag, S., Dehavay, F., EVRARD, S., Rousseaux, D., ADJETEY BAHUN, A., SEIDEL, L., LAFFINEUR, K., Lagrou, K., & HAYETTE, M.-P. (2020). Belgian national survey on tinea capitis: epidemiological considerations and highlight of terbinafine resistant T. mentagrophytes with a mutation on SQLE gene. Journal of Fungi. doi:10.3390/jof6040195

SACHELI, R., Menatong, X., Labarbe, C., Crützen, C., Harag, S., André, J., Evrard, S., Lagrou, K., Laffineur, K., Rousseaux, D., De Tollenaere, L., ADJETEY BAHUN, A., SEIDEL, L., & HAYETTE, M.-P. (2019). Belgian national survey on tinea capitis: epidemiology and molecular investigations. Mycoses, Supplement.

SACHELI, R., Tomé, C., ADJETEY BAHUN, A., DARFOUF, R., PALMEIRA, L., & HAYETTE, M.-P. (2019). Classification of dermatophytes by a multilocus phylogenetic approach based on Tef-1α, beta tubulin and ITS genes. Mycoses.

Melin, P., SACHELI, R., Lambotte, O., Hayette, M.-P., DESCY, J., HUYNEN, P., & MEEX, C. (2019). Group B streptococcus neonatal invasive infections in Belgium 2010-2017, and characterization of isolated strains. In I. C. INMIS, INMIS 2019 Abstract book.

Hayette, M.-P., De Tonnellaere, L., ADJETEY BAHUN, A., DARFOUF, R., & SACHELI, R. (23 May 2019). Evaluation of a screening agar for the detection of terbinafine resistance in dermatophytes isolates: preliminary results [Paper presentation]. Annual meeting of the Belgian Society of Human and Animal Mycology, Bruxelles, Belgium.

Van beckhoven Dominique, SACHELI, R., Patteet Sofie, HAYETTE, M.-P., & Lagrou Katrien. (2019). MYCOSES : Surveillance épidémiologique en Belgique 2016-2017. Brussels, Belgium: Sciensano.

MEEX, C., Defêche, J., DEVEY, A., DESCY, J., HAYETTE, M.-P., HUYNEN, P., SACHELI, R., & MELIN, P. (December 2018). Dépistage intrapartum du Streptococcus agalactiae par PCR GenePOC GBS DS [Poster presentation]. 38ème Réunion Interdisciplinaire de Chimiothérapie Anti-Infectieuse (RICAI).

MEEX, C., DEVEY, A., PHAM HONG, N., VAN GEET, M., DARFOUF, R., SACHELI, R., & MELIN, P. (February 2018). Prevalence and capsular-polysaccharide type distribution of colonizing group B streptococci (GBS) isolated from rectovaginal samples in pregnant women in Hanoï, Vietnam [Poster presentation]. 1st International Symposium on Streptococcus agalactiae Disease, Cape Town, South Africa.

Jacquinet, S., SACHELI, R., & MELIN, P. (2018). Vibrio cholerae (Cholera). In Maladies infectieuses liées à la consommation des aliments et de l'eau Surveillance épidémiologique 2015-2016 (pp. 82-84). Bruxelles, Belgium: WIV-ISP.

MELIN, P., LECOMTE, L., SACHELI, R., DESCY, J., HUYNEN, P., HAYETTE, M.-P., & MEEX, C. (2018). Group B Streptococcus neonatal invasive infections in Belgium, 2013-2016. In Abstract book of 1st ISSAD 2018.

SACHELI, R., MEEX, C., DESCY, J., HUYNEN, P., HAYETTE, M.-P., & MELIN, P. (2018). Characterization of Group B Streptococcus strains isolated from neonatal invasive diseases in Belgium, 2015. In abstract book of 1st ISSAD 2018.

SACHELI, R., MEEX, C., DESCY, J., HUYNEN, P., HAYETTE, M.-P., & MELIN, P. (2018). 2015 Surveillance of Group B Streptococcus strains isolated from invasive diseases among adults in Belgium : bacteriological and clinical characteristics. In Abstract book of 1st ISSAD 2018.

MEEX, C., DUPONT, A., SACHELI, R., DESCY, J., HUYNEN, P., HAYETTE, M.-P., & MELIN, P. (19 October 2017). Evaluation of the Xpert®GBS LB test (Cepheid) performed on antenatal screening LIM enrichment broth for detection of Streptococcus agalactiae or group B streptococcus, compared to the reference culture method [Paper presentation]. 20th Lancefield International Symposium on Streptococci and Streptococcal Diseases 2017, Denarau Island, Fiji.

MEEX, C., DEVEY, A., PHAM HONG, N., VAN GEET, M., DARFOUF, R., SACHELI, R., & MELIN, P. (October 2017). Prevalence and capsular-polysaccharide type distribution of colonizing group B streptococci (GBS) isolated from recto-vaginal samples in pregnant women in Hanoï, Vietnam [Poster presentation]. 20th Lancefield International Symposium on Streptococci and Streptococcal Diseases 2017, Denarau Island, Fiji.

SACHELI, R., DARFOUF, R., ADJETEY BAHUN, A., DESCY, J., HUYNEN, P., Patteet, S., Lagrou, K., & HAYETTE, M.-P. (October 2017). A 5-year survey of dermatophytes strains circulating in Belgium [Poster presentation]. 8th Trends in Medical Mycology, Belgrade, Serbia.

SACHELI, R., DESCY, J., MEEX, C., HUYNEN, P., HAYETTE, M.-P., & MELIN, P. (October 2017). Evolution of rate and genotypes of resistance to macrolide/lincosamide among invasive Group B Streptococcus (GBS): Development of a multiplex PCR tool for simultaneous detection of ErmB, ErmTr, MefA and LsaC resistance genes [Poster presentation]. 20th Lancefield International Symposium on Streptococci and Streptococcal Diseases 2017, Denarau Island, Fiji.

SACHELI, R., Ferrer Clusella, A., ADJETEY BAHUN, A., DARFOUF, R., MEEX, C., & HAYETTE, M.-P. (October 2017). Comparison of four methods for the identification of Trichophyton benhamiae strains: Maldi-Tof MS, ITS sequencing, Diversilab® system and DermaGenius® Multiplex Real-Time PCR kit [Poster presentation]. 8th trends in Medical Mycology, Belgrade, Serbia.

SACHELI, R., MEEX, C., DESCY, J., HUYNEN, P., HAYETTE, M.-P., & MELIN, P. (October 2017). Update of the characteristics of Group B Streptococci (GBS) colonizing pregnant women in Belgium: capsular-type distribution, pili characterization, antimicrobial susceptibility profile and Multiple Locus Sequence Types [Poster presentation]. 20th Lancefield International Symposium on Streptococci and Streptococcal Diseases 2017, Denarau Island, Fiji.

Aouini, I., SACHELI, R., DARFOUF, R., ADJETEY BAHUN, A., BONTEMS, S., MELIN, P., HAYETTE, M.-P., & rayes, A. (2017). Molecular characterization of five metalloproteases (Mep1-5) in Microsporum audouinii strains circulating in Belgium [Poster presentation]. 8th Trends in Medical Mycology, Belgrade, Serbia.

SACHELI, R., Utri, T., ADJETEY BAHUN, A., DARFOUF, R., & HAYETTE, M.-P. (09 April 2016). Genotypic characterization of T. mentagrophytes complex strains circulating in Belgium with the Diversilab® system [Poster presentation]. 26th ECCMID, Amsterdam, Netherlands.

SACHELI, R., Utri, T., Abdel-Sadick, H. A., ALFAGEME GONZALEZ, J., ADJETEY BAHUN, A., & HAYETTE, M.-P. (23 March 2016). Etude de dermatophytes isolés dans les services de revalidation et physiothérapie d’une structure hospitalière en région Liégeoise (Belgique) [Poster presentation]. Congrès 2016 de la société française de mycologie médicale Grenoble.

SACHELI, R., & HAYETTE, M.-P. (2016). Rapport du Centre National de Référence Mycoses Liège 2015. Bruxelles, Belgium: ISP-WIV.

SACHELI, R., DARFOUF, R., GRAIDE, H., ADJETEY BAHUN, A., Pateet, S., Lagrou, K., & HAYETTE, M.-P. (October 2015). A three year survey of dermatophytoses in Belgium. Mycoses, 58 (Supplement 4), 135.

SACHELI, R., Dekkers, C., GRAIDE, H., DARFOUF, R., ADJETEY BAHUN, A., MEEX, C., DESCY, J., HUYNEN, P., MELIN, P., André, J., ARRESE ESTRADA, J., & HAYETTE, M.-P. (October 2015). Epidemiological aspects and genotypic characterization of T.violaceum strains collected during a Belgian National survey on anthropophilic tinea. Mycoses, 58 (Supplement 4), 189.

HAYETTE, M.-P., & SACHELI, R. (September 2015). Dermatophytosis, Trends in Epidemiolgy and Diagnostic Approach. Current Fungal Infection Reports, 9 (3), 164-179. doi:10.1007/s12281-015-0231-4

SACHELI, R., Dekkers, C., DARFOUF, R., ADJETEY BAHUN, A., GRAIDE, H., MEEX, C., DESCY, J., HUYNEN, P., MELIN, P., ARRESE ESTRADA, J., & HAYETTE, M.-P. (May 2015). Epidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea [Poster presentation]. Sixth FEBS Advanced Lecture Course, Human Fungal Pathogens :Molecular Mechanisms of Host-Pathogen Interactions and Virulence, Nice, France.

SACHELI, R., Géron, B., Dekkers, C., DESCY, J., MEEX, C., HUYNEN, P., MELIN, P., & HAYETTE, M.-P. (28 April 2015). Epidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea [Poster presentation]. 25th ECCMID, Copenhague, Denmark.

Huyghe, A.* , Van Den Ackerveken, P.* , SACHELI, R., Prevot, P.-P., Thelen, N., Renauld, J., Thiry, M., Delacroix, L., Nguyen, L., & Malgrange, B. (2015). MicroRNA-124 Regulates Cell Specification in the Cochlea through Modulation of Sfrp4/5. Cell Reports, 13, 31-42. doi:10.1016/j.celrep.2015.08.054

SACHELI, R., & Hayette, M.-P. (2015). Rapport du Centre National de Référence Mycoses Liège 2014. Bruxelles, Belgium: ISP-WIV.

MEEX, C., SACHELI, R., DESCY, J., HAYETTE, M.-P., HUYNEN, P., KRIDELKA, F., IEVEN, M., & Melin, P. (12 November 2014). EVALUATION OF THE PRACTICABILITY OF A RAPID INTRAPARTUM SCREENING BASED STRATEGY FOR PREVENTION OF GROUP B STREPTOCOCCUS PERINATAL INFECTIONS USING A REAL TIME PCR PERFORMED BY MIDWIVES AS A POINT OF CARE TEST [Poster presentation]. XIX Lancefield International Symposium on Streptococci and Streptococcal Diseases, Buenos Aires, Argentina.

Dingemans, G., van den Bosch, M., HAYETTE, M.-P., Goethel, S., Rusu, V., SACHELI, R., Meis, J., Gaajetaan, G., & Simons, G. (May 2014). Development of a new commercial qPCR assay to detect and differentiate dermatophyte infections of the skin, nails and hair [Poster presentation]. 24th ECCMID.

Volvert, M.-L., Prevot, P.-P., Close, P., Laguesse, S., Pirotte, S., Hemphill, J., Rogister, F., Krusy, N., SACHELI, R., MOONEN, G., Deiters, A., Merkenschlager, M., CHARIOT, A., Malgrange, B., Godin, J., & Nguyen, L. (May 2014). MicroRNA Targeting of CoREST controls polarization of migrating cortical neurons. Cell Reports, 7 (4), 1168-83. doi:10.1016/j.celrep.2014.03.075

Mvumbi, D., BOREUX, R., SACHELI, R., Mvumbi, L., Bobanga, L., Situakibanza, H., Melin, P., Kayembe, J.-M., Kalala, L., De Mol, P., & Hayette, M.-P. (20 December 2013). Assessment of pfcrt 72-76 haplotypes eight years after chloroquine withdrawal in Kinshasa, Democratic Republic of Congo. Malaria Journal, 12, 459. doi:10.1186/1475-2875-12-459

SACHELI, R., DIMO, L., GRAIDE, H., MEEX, C., DESCY, J., HUYNEN, P., Melin, P., Arrese Estrada, J., & Hayette, M.-P. (01 October 2013). DNA fingerprinting using Diversilab system for genotyping characterization of Microsporum audouinii and Trichophyton violaceum. Mycoses, 56 (Supplement S3), 99. doi:10.1111/myc.12121_1

MELIN, P., Dodémont, M., Sarlet, G., SACHELI, R., DESCY, J., MEEX, C., HUYNEN, P., & HAYETTE, M.-P. (2013). Improvement of transport condition of swabs for group B streptococcal (GBS) screening. In Program and Abstract of the 53rd Intersciences Conference on Antimicrobial Agents and Chemotherapy. Washington, USA: ASM. Washington, United States: ASM.

Melin, P., SACHELI, R., Sarlet, G., MEEX, C., DESCY, J., HUYNEN, P., & HAYETTE, M.-P. (2013). Surveillance of serotypes and antimicrobial susceptibility profile in group B streptococcus (GBS) in Belgium. In Program and Abstract of the 53rd Intersciences Conference on Antimicrobial Agents and Chemotherapy. Washington, USA: ASM. Washington, United States: ASM.

Melin, P., & SACHELI, R. (2013). Centre National de référence Streptococcus agalactiae 2012. Bruxelles, Belgium: ISP-WIV.