Publications of Céline KEMPENEERS
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See detailPrimary Ciliary Dyskinesia Center in CHU of Liège
KEMPENEERS, Céline ULiege

Speech/Talk (2020)

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See detailThe influence of the conservation of respiratory epithelial samples on ciliary functional analysis
Bricmont, Noémie ULiege; Benchimol, Lionel ULiege; POIRRIER, Anne-Lise ULiege et al

Conference (2020)

Background: Primary ciliary dyskinesia(PCD) is an inherited otosinopulmonary ciliopathy in which respiratory cilia are stationary, or beat in a dyskinetic manner. Digital high speed videomicroscopy(DHSV ... [more ▼]

Background: Primary ciliary dyskinesia(PCD) is an inherited otosinopulmonary ciliopathy in which respiratory cilia are stationary, or beat in a dyskinetic manner. Digital high speed videomicroscopy(DHSV) allows ciliary functional analysis(CFA), including beat frequency(CBF) and pattern(CBP). DHSV is highly sensitive and specific for PCD diagnosis, but lacks standardization and evidence-based data. The technical process of ciliated epithelial samples before CFA varies among studies. Of importance, delay after sampling and temperature for samples conservation may impact CFA. However, these parameters have never been compared. Aims: To evaluate the stability of ciliary function over time when nasal brushing samples are conserved either in the fridge, or at room temperature. Methods: Ciliated epithelial samples were obtained by brushing the inferior nasal turbinate from 5 non-smoking healthy subjects. The samples were divided equally, and conserved either at 4°C or at 22°C. Beating cilia were recorded using DHSV at 37°C immediately(T0, then 9 hours after sampling(T9). CFA was assessed by CBF and the percentage of normal CBP. Results: There was no significant difference between CFA performed immediately or 9 hours after sampling, regardless of the sample storage temperature. Conclusion: This pilot study suggested that the stability of CFA may be preserved for 9 hours at 22°C and at 4°C. Larger studies are needed to confirm these preliminary results, notably in pathological conditions. [less ▲]

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See detailProtocole de prise en charge de la bronchiolite
KEMPENEERS, Céline ULiege

Speech/Talk (2019)

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See detailTotal absence of the pericardium associated with hypogammaglobulinemia and bronchiectasis in a girl
KINUANI MULASI, Rachel ULiege; BRUYERE, Pierre-Julien ULiege; Schoysman, Laurent et al

in Paediatric Reports (2019), 11(8250),

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See detailPrimary Ciliary Dyskinesia in CHU of Liège
KEMPENEERS, Céline ULiege

Speech/Talk (2019)

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See detailCiliary Functional Analysis: Beating a path towards standardisation
KEMPENEERS, Céline ULiege; Seaton, Claire; Garcia Espinosa, Bernardo et al

Poster (2019, October 18)

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See detailCiliary functional analysis: Beating a path towards standardization.
KEMPENEERS, Céline ULiege; Seaton, Claire; Garcia Espinosa, Bernardo et al

in Pediatric Pulmonology (2019), 54(10), 1627-1638

Primary ciliary dyskinesia is an inherited disorder in which respiratory cilia are stationary, or beat in a slow or dyskinetic manner, leading to impaired mucociliary clearance and significant ... [more ▼]

Primary ciliary dyskinesia is an inherited disorder in which respiratory cilia are stationary, or beat in a slow or dyskinetic manner, leading to impaired mucociliary clearance and significant sinopulmonary disease. One diagnostic test is ciliary functional analysis using digital high-speed video microscopy (DHSV), which allows real-time analysis of complete ciliary function, comprising ciliary beat frequency (CBF) and ciliary beat pattern (CBP). However, DHSV lacks standardization. In this paper, the current knowledge of DHSV ciliary functional analysis is presented, and recommendations given for a standardized protocol for ciliary sample collection and processing. A proposal is presented for a quantitative and qualitative CBP evaluation system, to be used to develop international consensus agreement, and future DHSV research areas are identified. [less ▲]

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See detailChronic cough in Pediatrics: Algorithms
KEMPENEERS, Céline ULiege

Speech/Talk (2018)

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See detailToux chronique chez l’enfant
KEMPENEERS, Céline ULiege

Speech/Talk (2018)

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See detailTo beat, or not to beat, that is question! The spectrum of ciliopathies.
Kempeneers, Céline ULiege; Chilvers, Mark A.

in Pediatric Pulmonology (2018), 53(8), 1122-1129

Cilia are widely distributed throughout the human body, and have numerous roles in physiology, development, and disease. Ciliary ultrastructure is complex, consisting of nine parallel microtubules ... [more ▼]

Cilia are widely distributed throughout the human body, and have numerous roles in physiology, development, and disease. Ciliary ultrastructure is complex, consisting of nine parallel microtubules doublets, with or without motor dynein arms and a central pair of microtubules. Classification of cilia has evolved over time, and currently, four main classes are described: motile and non-motile cilia with a "9 + 2" structure, and motile and non-motile cilia with a "9 + 0" structure, which depend on the presence or absence of dynein arms and a central pair. Ciliopathies are inherited multisystem disorders of cilia, and may present with a varied spectrum of genotypes and phenotypes. Motor and sensory ciliopathies were historically considered as distinct dysfunctions of motile and non-motile cilia, but recent data indicate that the classical features of motor and sensory cilia may overlap. [less ▲]

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See detailCiliary Videomicroscopy: Standardisation and Use in Ciliary Functional Analysis
KEMPENEERS, Céline ULiege

Scientific conference (2018, May 17)

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See detailCiliary functional analysis using ciliary videomicroscopy: time for a standardisation
KEMPENEERS, Céline ULiege; Seaton, Claire; Garcia Espinosa, Bernardo et al

Conference (2018, February)

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See detailResponse.
KEMPENEERS, Céline ULiege; Seaton, Claire; Chilvers, Mark A.

in CHEST (2017), 152(6), 1349-1350

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See detailCiliary Videomicroscopy: Standardisation and Use in Ciliary Functional Analysis
KEMPENEERS, Céline ULiege

Doctoral thesis (2017)

Cilia are hair-like organelles, distributed widely throughout the human body, and may be either motile or sensory. Primary ciliary dyskinesia is an inherited motile ciliopathy, in which respiratory cilia ... [more ▼]

Cilia are hair-like organelles, distributed widely throughout the human body, and may be either motile or sensory. Primary ciliary dyskinesia is an inherited motile ciliopathy, in which respiratory cilia are stationary, or beat in a slow or dyskinetic manner. This leads to impaired mucociliary clearance and significant sinopulmonary disease. The diagnosis of primary ciliary dyskinesia is challenging, and a panel of different tests are required. Digital high speed videomicroscopy is one technique which allows real time analysis of complete ciliary function, including ciliary beat frequency and beat pattern, and allows beating cilia to be visualized in three different planes. Despite it being highly sensitive and specific test for diagnosing primary ciliary dyskinesia, digital high speed videomicroscopy lacks standardization. A review of the current state of the art concerning digital high speed videomicroscopy ciliary functional assessment was conducted, and the methodology between different laboratories compared. The data was used to generate recommendations concerning digital high speed videomicrosopy methodology, evaluation, and interpretation. The variability of ciliary beat pattern within healthy respiratory ciliated epithelium, and reproducibility in manual beat pattern analysis, was evaluated using digital high speed videomicroscopy. The results highlighted issues with single and limited manual beat pattern analysis. Compared to the sideways profile, in healthy subjects, cilia beating toward the observer and from above profile were found to be less sensitive in detecting ciliary dyskinesia. However, these beating profiles may be used to calculate ciliary beat frequency. In addition, the evaluation of five beat cycles to measure ciliary beat frequency is as accurate as ten beat cycles. Summary Standardized protocols for ciliary functional analysis using digital high speed videomicroscopy need to be established and applied in different laboratories with appropriate reference ranges. Ciliary beat pattern is not uniform within healthy respiratory ciliated epithelium, emphasizing the risk of limited beat pattern analysis. Further work is needed to evaluate the use of the different beat profiles for evaluation of ciliary function in patients with primary ciliary dyskinesia. [less ▲]

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See detailVariation of Ciliary Beat Pattern in Three Different Beating Planes in Healthy Subjects.
Kempeneers, Céline ULiege; Seaton, Claire; Chilvers, Mark A.

in CHEST (2017), 151(5), 993-1001

BACKGROUND: Digital high-speed video microscopy (DHSV) allows analysis of ciliary beat frequency (CBF) and ciliary beat pattern (CBP) of respiratory cilia in three planes. Normal reference data use a ... [more ▼]

BACKGROUND: Digital high-speed video microscopy (DHSV) allows analysis of ciliary beat frequency (CBF) and ciliary beat pattern (CBP) of respiratory cilia in three planes. Normal reference data use a sideways edge to evaluate ciliary dyskinesia and calculate CBF using the time needed for a cilium to complete 10 beat cycles. Variability in CBF within the respiratory epithelium has been described, but data concerning variation of CBP is limited in healthy epithelium. This study aimed to document variability of CBP in normal samples, to compare ciliary function in three profiles, and to compare CBF calculated over five or 10 beat cycles. METHODS: Nasal brushing samples from 13 healthy subjects were recorded using DHSV in three profiles. CBP and CBF over a 10-beat cycle were evaluated in all profiles, and CBF was reevaluated over five-beat cycles in the sideways edges. RESULTS: A uniform CBP was seen in 82.1% of edges. In the sideways profile, uniformity within the edge was lower (uniform normal CBP, 69.1% [sideways profile]; 97.1% [toward the observer], 92.0% [from above]), and dyskinesia was higher. Interobserver agreement for dyskinesia was poor. CBF was not different between profiles (P = .8097) or between 10 and five beat cycles (P = .1126). CONCLUSIONS: Our study demonstrates a lack of uniformity and consistency in manual CBP analysis of samples from healthy subjects, emphasizing the risk of automated CBP analysis in limited regions of interest and of single and limited manual CBP analysis. The toward the observer and from above profiles may be used to calculate CBF but may be less sensitive for evaluation of ciliary dyskinesia and CBP. CBF can be measured reliably by evaluation of only five-beat cycles. [less ▲]

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See detailLa dyskinésie ciliaire
KEMPENEERS, Céline ULiege

Speech/Talk (2016)

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See detailPrimary Ciliary Dyskinesia
KEMPENEERS, Céline ULiege

Conference given outside the academic context (2016)

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See detailPrimary Ciliary Dyskinesia Diagnosis : variation of the Ciliary Beat Pattern in Healthy Subjects
KEMPENEERS, Céline ULiege; Seaton, Claire; Chilvers, Mark

Conference (2016, March)

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