Publications of Romy GADISSEUR
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See detailNouvelles perspectives cliniques avec ImmunoCAP ISAC
GADISSEUR, Romy ULiege

Conference (2020, February 20)

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See detailDetection of wheat allergens using 2D Western blot and mass spectrometry
Courtois, Justine ULiege; BERTHOLET, Catherine ULiege; Tollenaere, S. et al

in Journal of Pharmaceutical and Biomedical Analysis (2020), 178

Background: Wheat allergy is relatively common and the associated clinical manifestations depend on the involved molecular allergens as well as on the way of exposure. Different symptoms have been ... [more ▼]

Background: Wheat allergy is relatively common and the associated clinical manifestations depend on the involved molecular allergens as well as on the way of exposure. Different symptoms have been described: wheat-dependent exercise-induced anaphylaxis (WDEIA), atopic dermatitis (AD) and pollen rhinitis (PR). Traditional diagnostic methods do not allow accurate molecular identification of the allergens that are essential for risk assessment and for the choice of the most adapted treatment. Methods: Standardized total protein extracts obtained from wheat seeds were separated by 2D electrophoresis. Twenty-one sera with high wheat-specific immunoglobulin E (sIgE) levels were classified into three patients groups based on their clinical profile. These sera were tested by Western blot on 2D separated standardized wheat protein extract and their sIgE sensitization profiles were compared. Results: Specific sensitization profiles were identified for each phenotype group. For WDEIA, protein spots around 37 kDa (pH 6-9) and 37–50 kDa (pH 5-6) were identified. For AD, spots were observed around 50 kDa (pH 9), 10 kDa (pH 9) and 20 to 75 kDa (pH3). For PR, specific spots were located around 90 kDa (pH 9). The mass spectrometry (UHPLC-MS/MS) analysis of these identified spots pointed out several potential interesting allergens: Tri a 26, Tri a bA, Tri a 34, Tri a tritin. Conclusions: The present study allowed the identification of different protein areas specific to these studied groups. The protein spots of interest were identified by UHPLC-MS/MS. It has been possible to establish a link between a specific symptomatology and the newly identified responsible allergens. © 2019 Elsevier B.V. [less ▲]

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See detailNew approaches to manage hymenoptera venom allergic patients at risk of anaphylaxis
GADISSEUR, Romy ULiege

Conference (2019, December 14)

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See detailAllergies croisées crustacés et insectes comestibles
GADISSEUR, Romy ULiege

Conference (2019, December 14)

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See detailRisk of allergenic transmissions linked to human insect consumption
GADISSEUR, Romy ULiege

Scientific conference (2019, December 03)

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See detailGood Clinical Practice for in-vitro diagnosis of food allergy
GADISSEUR, Romy ULiege

Conference (2019, September 21)

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See detailWEBINAR Intérêt de la puce ImmunoCAP ISAC
GADISSEUR, Romy ULiege

in GADISSEUR, Romy (Ed.) Intérêt de la puce ImmunoCAP ISAC (2019)

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See detailBetula verrucosa may be taken as a marker allergen for Tx5 tree mix
GADISSEUR, Romy ULiege; LUKAS, Pierre ULiege; Cavalier, Etienne ULiege

in Allergy (2019, June 03), 74(106), 130-331

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See detailPhleum pratense may be taken as a marker allergen for Gx3 grass mix
GADISSEUR, Romy ULiege; LUKAS, Pierre ULiege; Cavalier, Etienne ULiege

in Allergy (2019, June 03), 74(106), 101-331

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See detailProfil de sensibilisation de patients allergiques au sein d'une population entomophage à Niamey au Niger
GADISSEUR, Romy ULiege; Hamidou, Tahirou

Poster (2019, April 17)

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See detailHighlighting of allergens found in edible crickets (Ornithachris turbida cavroisi) from Niger using 2D Western blot – a clinical case report
Courtois, Justine ULiege; Hamidou, Tahirou; Van der Brempt, Xavier et al

Conference (2018, December 01)

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See detailDiagnostic accuracy of three automated urine analyzers compared to urine culture
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine culture is an important diagnosis method of urinary tract infection. However, it is time-consuming and results aren’t available quickly. Automated urine analyzers have been developed to screen urine ... [more ▼]

Urine culture is an important diagnosis method of urinary tract infection. However, it is time-consuming and results aren’t available quickly. Automated urine analyzers have been developed to screen urine samples more rapidly. The goal of this study was to compare three automated analyzers to urine culture: the Atellica UAS 800 (Siemens, Munich, Germany), the UF-4000 (Sysmex, Kobe, Japan) and the SediMAX (Menarini, Florence, Italy). We first validated each analyzer. We analyzed then 318 samples with the three analyzers within 2 hours after sample reception. An urine aliquot was collected before sediment analysis for bacteria culture. Ten microliters of un-centrifuged urine was inoculated on blood agar and CLED agar plates, then they were incubated aerobically at 36°C for 24 h. Bacteria count of each analyzer was compared to urine culture to determine diagnostic accuracy. The result was considered positive when the bacteria growth reached 104 CFU/ml. We also used the results of leukocytes and nitrites results from the strip measurement to improve the accuracy. The abilities of the UF-4000 to distinguish Gram positive (GP) from Gram negative (GN) bacteria, and of the UAS 800 to identify rod and cocci, were determined. [less ▲]

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See detailUrine sediment analysis: comparison of three automated analyzers to manual microscopy
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine microscopic analysis is an old method that reveals information about kidney health. Several automated analyzers, that are less time-consuming, are currently available, but manual microscopy is still ... [more ▼]

Urine microscopic analysis is an old method that reveals information about kidney health. Several automated analyzers, that are less time-consuming, are currently available, but manual microscopy is still the gold-standard method. The goal of this study was to validate and compare three automated analyzers to manual microscopy: the Atellica UAS800 (Siemens, Munich, Germany), the UF-4000 (Sysmex, Kobe, Japan) and the SediMax (Menarini, Florence, Italy). We first validated each analyzer. A total of 359 samples were analyzed with the three analyzers and with a manual microscope within 2 hours. Two trained reviewers used a microscope with bright field, contrast phase and polarized light to identify urine elements. The diagnostic accuracy was determined thanks to microscopy results. [less ▲]

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See detailComparison of three automated strip analyzers to Cobas 800 for the analysis of glucose, proteins, albumin and creatinine
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine glucose, proteins, albumin and creatinine are measured for the screening of diabetes and renal diseases. The automated strip analyzers are used for quick screening of large populations. The goal of ... [more ▼]

Urine glucose, proteins, albumin and creatinine are measured for the screening of diabetes and renal diseases. The automated strip analyzers are used for quick screening of large populations. The goal of this study was to compare a more accurate method, the Cobas 8000 (Roche, Bale, Switzerland) to three automated analyzers: the Clinitek Novus (Siemens, Munich, Germany), the UC-3500 (Sysmex, Kobe, Japan) and the AutionMax (Menarini, Florence, Italy). A total of 284 urine samples were prospectively collected for the comparison. The samples were analyzed on the three analyzers within 2 hours. Before the analysis of samples by each method, an aliquot was frozen at -80°C. All samples were then defrost and analyzed in one batch with the Cobas 8000 (Roche, Bale, Switzerland) within the month. The diagnostic accuracy was determined thanks to the results of the Cobas 8000. However, the creatinine and albumin cannot be assessed with the AutionMax. [less ▲]

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See detailRaman chemical imaging, a new tool in kidney stone structure analysis: Case-study and comparison to Fourier Transform Infrared spectroscopy
Castiglione, Vincent ULiege; Sacre, Pierre-Yves ULiege; Cavalier, Etienne ULiege et al

Poster (2018, November 16)

The kidney stone’s structure might provide clinical information in addition to the stone composition. The Raman chemical imaging is a technology used for the production of two- dimension maps of the ... [more ▼]

The kidney stone’s structure might provide clinical information in addition to the stone composition. The Raman chemical imaging is a technology used for the production of two- dimension maps of the constituents’ distribution in samples. We aimed at determining the use of Raman chemical imaging in urinary stone analysis. Fourteen calculi were analyzed by Raman chemical imaging using a confocal Raman micro- spectrophotometer. They were selected according to their heterogeneous composition and morphology. Raman chemical imaging was performed on the whole section of stones. Once acquired, the data were baseline corrected and analyzed by MCR-ALS. Results were then compared to the spectra obtained by Fourier Transform Infrared spectroscopy. [less ▲]

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See detailHighlighting of an allergenic cross-reaction between crustaceans, house dust mites and crickets using 2D Western blot
Courtois, Justine ULiege; Quinting, Birgit; Van der Brempt, Xavier et al

Poster (2018, November 16)

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See detailHighlighting of allergens found in edible crickets (Ornithachris turbida cavroisi) from Niger using 2D Western blot – a clinical case report
Courtois, Justine ULiege; Hamidou, Tahirou; Van der Brempt, Xavier et al

Poster (2018, November 16)

Detailed reference viewed: 30 (5 ULiège)
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See detailClinical data on rare Sulfamethoxazole crystalluria assessed by Fourier Transform Infrared Spectrophotometry
CASTIGLIONE, Vincent ULiege; CAVALIER, Etienne ULiege; GADISSEUR, Romy ULiege

in Data in Brief (2018)

The data contained in this article are related to the article entitled “Case report: Uncommon Sulfamethoxazole Crystalluria” [1]. Sulfamethoxazole crystalluria is very rare and crystals identification is ... [more ▼]

The data contained in this article are related to the article entitled “Case report: Uncommon Sulfamethoxazole Crystalluria” [1]. Sulfamethoxazole crystalluria is very rare and crystals identification is complex [2], [3]. We identified seven patients with uncommon urine crystals that were composed of N-Acetyl-Sulfamethoxazole. Three of the patients developed an acute renal failure simultaneously to crystalluria. Hence, this article describes the method of crystals identification thanks to infrared spectroscopy. The relevant clinical data of patients, including medical history, drug dosage and urine parameters related to the crystalluria are presented. [less ▲]

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