Publications of Pascale HUYNEN
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See detailVirus de l’Immunodéficience acquise.
Huynen, Pascale ULiege

Scientific conference (2019, December 13)

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See detailInfections sexuellement transmissibles
Huynen, Pascale ULiege; LIBOIS, Agnès

Scientific conference (2019, October 03)

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See detailEvolution in viroloy and the effect on organ donation
HUYNEN, Pascale ULiege

Conference (2019, September 26)

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See detailGroup B streptococcus neonatal invasive infections in Belgium 2010-2017, and characterization of isolated strains.
Melin, Pierrette ULiege; SACHELI, Rosalie ULiege; Lambotte, Olivia et al

in INMIS, International Committee (Ed.) INMIS 2019 Abstract book (2019, September)

Introduction/Background & Aims: Where intrapartum antibiotic-prophylaxis (IAP) is given to pregnant women colonized with Group B Streptococcus (GBS), the incidence of neonatal early-onset disease (EOD ... [more ▼]

Introduction/Background & Aims: Where intrapartum antibiotic-prophylaxis (IAP) is given to pregnant women colonized with Group B Streptococcus (GBS), the incidence of neonatal early-onset disease (EOD) has been successfully reduced; nevertheless, GBS is still the leading cause of severe disease among newborns, notably because the incidence of GBS late-onset disease (LOD) is not affected by IAP. Another strategy such as maternal immunization for prevention of both EOD/LOD is highly desirable worldwide. Aiming to describe GBS epidemiology and characterization of relevant epidemiological markers for vaccine development, surveillance of isolates causing neonatal disease is needed. We provide here results from the Belgian surveillance organized by the National Reference Centre(NRC). Methods: A total of 292 strains of GBS isolated from blood culture/cerebro-spinal fluid of newborns with invasive disease (149 EOD; 143 LOD) were sent to NRC by laboratories of a surveillance network, through years 2010-2017. Capsular-polysaccharide (CPS)-typing and pili-typing were performed with multiplex PCR assays. Multilocus sequence-typing and assignment to the hypervirulent clonal-complex (CC)17 was determined. Results: CPS type III isolates were responsible for 38.9% (n=58) of EOD cases, followed mainly by types Ia, V and II (22.1%, 18.1%, 8.1%). LOD cases were mainly caused by type III isolates (n=107, 74.8%), followed by types Ia (12.6%), V, Ib, IV and II (4.2%, 3.5%,2.8%, 2.1%). These distributions did not vary during the study period. A pili type was assigned to all isolates: at least one pili gene, PI2a, PI2b, or a combination of genes PI1-PI2a and PI1-PI2b. In 2016-2017, the hypervirulent-clone CC17 accounted for 33.3% of EOD (70.4% of type III) and 67% of LOD (89% of type III). Conclusions: The Belgian CPS distributions of isolates from EOD/LOD were similar to European data. One or 2 of 3 pilus-genes were detected in all isolates. CPS type III was predominant in both EOD/LOD and was mainly represented by CC-17 strains. [less ▲]

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See detailTORCH Serology and pregnancy
HUYNEN, Pascale ULiege

Conference (2019, June)

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See detailLa maladie de Lyme : aspects biologiques
HUYNEN, Pascale ULiege

Conference (2019, May 23)

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See detailLe diagnostic de la maladie de Lyme n'est pas simple: le point avec le Dr HUYNEN du CHU de Liège
HUYNEN, Pascale ULiege

Article for general public (2019)

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See detailMaladie de Lyme : la clinique prime sur la sérologie
HUYNEN, Pascale ULiege

Article for general public (2019)

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See detailNorovirus enteritis
HUYNEN, Pascale ULiege

Scientific conference (2019, April 26)

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See detailVirologie Clinique: sérologie infectieuse et détection antigénique
HUYNEN, Pascale ULiege

Learning material (2019)

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See detailLes infections digestives et leur prévention (hors C.difficile)
HUYNEN, Pascale ULiege; DUMAZY, Axelle ULiege

Scientific conference (2019, March 20)

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See detailBorréliose de Lyme
HUYNEN, Pascale ULiege; LEONARD, Philippe ULiege

Scientific conference (2019, March 11)

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See detailLe norovirus humain : contribution à la détection, à la caractérisation et l'épidémiologie clinique et moléculaire.
HUYNEN, Pascale ULiege

Doctoral thesis (2019)

Noroviruses are non-enveloped single stranded RNA viruses. The norovirus genus contains seven genogroups (G) including genogroups I, II and IV which infect humans. Human noroviruses (NoV) are recognized ... [more ▼]

Noroviruses are non-enveloped single stranded RNA viruses. The norovirus genus contains seven genogroups (G) including genogroups I, II and IV which infect humans. Human noroviruses (NoV) are recognized as worldwide leading pathogens causing viral gastro-enteritis, and as the main pathogenic agent responsible for foodborne gastro-enteritis. Ten years ago, NoV were not well known by microbiologists and clinicians in Belgium. The commercial detection methods now available did not yet exist and NoV were not routinely detected in patients. In particular, the University Hospital of Liège did not have a method for NoV detection in patients. As a prerequisite to further NoV study, development and implementation of a NoV detection method was necessary. This work describes the development of a quantitative real time PCR, allowing the detection of pathogenic human NoV genogroups I, II and IV in a single reaction. When dealing with a gastro-enteritis outbreak of suspected viral etiology, a rapid NoV identification is the key to effective control of viral transmission. To enable this, a given diagnostic laboratory needs a simple and rapid method that does not require expensive or complicated equipment. Despite molecular diagnosis methods remaining the gold standard for NoV detection, a rapid immunologic test for viral antigen detection was also evaluated and implemented in this study. In the context of a gastro-enteritis outbreak, these easy-to use tests are a rapid and efficient diagnosis method which can leading to quick implementation of prevention strategies. NoV outbreaks are difficult to control, mainly since NoV are highly contagious and resistant in the environment, complicating complete disinfection and leading to recurrent outbreaks. In hospitals in particular, NoV outbreaks can have a real public health impact and can lead to high costs. The rapid implementation efficient hygiene measures is essential to limit the size of such outbreaks. In developing countries, gastro-enteritis remains an important cause of morbidity and mortality. In Africa in particular, NoV detection is not or not often performed due to the prohibitive cost; the impact of NoV infections is thus probably under-estimated. In this continent very few studies have been realized. Via a collaboration with the Bobo Dioulasso health care center in Burkina Faso, the second study performed in the context of this thesis addressed this lack of information and allowed the investigation of molecular epidemiology of NoV strains circulating in 418 children from this region. While NoV GII.4 is typically identified as the predominant genotype in most studies performed in Africa, a large circulating strain diversity was observed in the study realized in Burkina Faso, with a surprisingly high NoV GI proportion. The number of NoV carriers observed in asymptomatic young children was high. These children could therefore play a reservoir role for NoV infections. The frequent contacts with NoV and exposure to numerous different strains, probably due to limited access to drinking water and a low hygiene level, could explain the host protection and the high carrier level in these countries. After a short incubation period of 24 to 72 hours, the usual clinical presentation of a NoV infection in a healthy patient is a gastro-enteritis, benign in most cases and spontaneously resolved in two to three days. In immunocompromised patients, clinical evolution of a NoV infection in not the same. In these patients, NoV are responsible for persistent and chronic diarrhea, and often remain under-diagnosed. Such persistent infections have long been described in hematopoietic stem cell transplant patients and, more recently, in solid organ transplant patients. Chronic diarrhea has also been reported in patients with high immunosuppression due to HIV infection. In a university hospital where more than 2000 patients have been transplanted up to the time of writing, a NoV study in this patient category was indicated. Via an effective collaboration with the Service of Nephrology and financial support obtained from the University Hospital of Liège, a prospective study was realized during more than four years in 117 kidney transplanted patients, presenting or not gastro-intestinal symptoms. In this study, prolonged viral excretion was observed in symptomatic kidney transplanted patients but also in asymptomatic patients, with maximal excretion duration of more than to eight and six months respectively. During these prolonged excretions, viral loads remained high in some patients, which could represent a potential source of viral transmission. NoV detection with molecular biology methods should be a part of the exploration of persistent digestive troubles in immunocompromised patients to allow differentiation with other origins of digestive and improvement of clinical care of these patients. Indeed, solving NoV chronic infections in immunocompromised, transplant patients is linked with the immune system recovery. This study has shown a positive impact of immunosuppressive treatment reduction on symptomatology. The study of NoV has undoubtily contributed to develop specific skills in this field, and had improved knowledge concerning this enteropathogen that is still underinvestigated in patients. [less ▲]

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See detailÉpidémie nosocomiale à norovirus : à propos d'une expérience hospitalière
HUYNEN, Pascale ULiege; MAUROY, Axel; LAMBERT, Nathalie et al

in Revue Médicale de Liège (2019), 74(2), 86-89

Human noroviruses (NoV) are the main pathogenic agents worldwide responsible for viral sporadic and epidemic gastroenteritis worldwide. A gastroenteritis outbreak broke out in patients hospitalized in ... [more ▼]

Human noroviruses (NoV) are the main pathogenic agents worldwide responsible for viral sporadic and epidemic gastroenteritis worldwide. A gastroenteritis outbreak broke out in patients hospitalized in several wards located in two different floors of a hospital in Liege, Belgium. The objective was to determine whether a same NoV strain would be involved in the two different floors, and to explore how this outbreak would have spread from a floor to the other. Stool samples from patients and healthcare workers were collected, as well as data from medical files. NoV detection, quantification and characterization were performed using molecular biology methods. A same NoV strain, from genotype GII.4, was detected in two patients hospitalized on the two different floors. This finding allowed to conclude that a same outbreak spread in the two floors, probably due to movements of common healthcare workers. A rapid NoV detection during outbreak is important in the aim to rapidly implement hygiene measures to limit the size of the outbreak. [less ▲]

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See detailVirologie et syndromes cliniques
HUYNEN, Pascale ULiege

Scientific conference (2019, January 16)

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See detailLes norovirus, ces entéro-pathogènes épidémiques mondiaux méconnus
Huynen, Pascale ULiege; Mauroy, A.; Melin, Pierrette ULiege et al

in Revue Médicale de Liège (2019), 74(1), 41-46

Discovered in the 1970s, human noroviruses (NoV) are the leading cause of foodborne disease and gastroenteritis outbreaks worldwide. NoV affect people of all ages. In children less than 5 years old ... [more ▼]

Discovered in the 1970s, human noroviruses (NoV) are the leading cause of foodborne disease and gastroenteritis outbreaks worldwide. NoV affect people of all ages. In children less than 5 years old, despite rotavirus remains the main enteropathogen responsible for viral gastroenteritis, NoV become the first etiological virus in countries where the rotavirus vaccine was introduced. Treatment of viral gastroenteritis is symptomatic.The key element in front of NoV infection is limiting their transmission. A rapid NoV detection during outbreak is important in the aim to rapidly implement hygiene measures to limit the size of the outbreak. Prevention of NoV infections relies on the use of adequate hand hygiene measures and disinfection of contaminated environmental surfaces. In face of an acute gastroenteritis outbreak, the early NoV identification with rapid laboratory tests or molecular biology methods is needed in the aim to implement as soon as possible hygiene measures to limit the size of the NoV outbreak. Due to antigenically diverse NoV strains and the lack of long term immunity, the development of an effective vaccine is difficult. © Revue Medicale de Liege. All rights reserved. [less ▲]

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See detailProfesseur invité
Huynen, Pascale ULiege

Learning material (2019)

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See detailNorovirus enteritis
HUYNEN, Pascale ULiege

Learning material (2019)

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See detailEvaluation of the Abbott RealTime quantitative CMV and EBV assays using the maxCycle protocol in a laboratory automation context
Bontems, Sébastien ULiege; BOREUX, Raphaël ULiege; CAPRARO, Valérie ULiege et al

in Journal of Virological Methods (2019), 270

Real-time PCR are often used for the diagnosis and monitoring of Cytomegalovirus (CMV)and Epstein-Barr virus (EBV)infections in susceptible populations. In this context, we evaluated the analytical ... [more ▼]

Real-time PCR are often used for the diagnosis and monitoring of Cytomegalovirus (CMV)and Epstein-Barr virus (EBV)infections in susceptible populations. In this context, we evaluated the analytical performances of the Abbott RealTime CMV/EBV maxCycle protocol automated on the m2000 platform (Abbott). It was compared to our routinely-used procedure consisting of a NucleoMag® DNA extraction automated on a STARlet platform followed by manually processed CMV and EBV quantitative real-time PCR (Diagenode). In this study, we showed that both EBV assays exhibited a similar sensitivity but with a better precision for the EBV Abbott RealTime assay. For the CMV performances, the Abbott assay was more sensitive and more precise than our routine method. The use of WHO International Standards also indicated a slight underestimation of the viral loads (−0.25 log10 IU/mL and −0.21 log10 IU/mL for CMV and EBV assays respectively)while these were rather overestimated with the Starlet/Diagenode method (0.48 log10 IU/mL and 0.19 log10 IU/mL for CMV and EBV assays respectively). These trends were confirmed using relevant whole-blood clinical samples and external quality controls. The workflows were also compared and we highlighted a significant technician hands-on time reduction (−63%)using the Abbott CMV/EBV maxCycle automated protocol. © 2019 Elsevier B.V. [less ▲]

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