Publications of Caroline LE GOFF
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See detailLe COVID-19 pourrait être dangereux pour le coeur des sportifs
ANCION, Arnaud ULiege; LE GOFF, Caroline ULiege; Kaux, Jean-François ULiege

Article for general public (2020)

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See detailThe pathway through LC-MS method development: in-house or ready-to-use kit-based methods?
LE GOFF, Caroline ULiege; Farre Segura, Jordi ULiege; STOJKOVIC, Violeta ULiege et al

in Clinical Chemistry and Laboratory Medicine (2020)

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See detailImpact of Metformin on vitamin B12 status
VRANKEN, Laura ULiege; FONTAINE, Corentin ULiege; VALDES SOCIN, Hernan Gonzalo ULiege et al

Poster (2019, November 15)

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See detailEvaluation of Metformin impact on vitamin B12 status
VRANKEN, Laura ULiege; FONTAINE, Corentin ULiege; LUKAS, Pierre ULiege et al

Poster (2019, May)

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See detailComparison of cardiac biomarker dynamics in marathon, semimarathon and untrained runners: what is the impact on results interpretation?
Le Goff, Caroline ULiege; Lennartz, Lieselotte; VRANKEN, Laura ULiege et al

in Journal of Laboratory and Precision Medicine (2019), 4(6), 10

Background: Cardiac biomarkers elevations have been described after running exercise. Objective of our study was to check cardiac biomarker dynamics in well trained athletes and untrained middle aged ... [more ▼]

Background: Cardiac biomarkers elevations have been described after running exercise. Objective of our study was to check cardiac biomarker dynamics in well trained athletes and untrained middle aged apparently healthy men and to highlight the impact on the lab results interpretation in emergency department. Methods: Cardiac biomarkers for ischemic condition, cardiac stretch and fibrotic processes were tested in different type of runners before, directly after and 3 hours after running. Markers for inflammation, muscle disease and renal function were also measured. Results: Cardiac biomarker levels between groups were not statistically different in the pre-exercise samples for natriuretic peptides [B-type natriuretic peptide (BNP), N-terminal Pro BNP (NT-ProBNP)] and galectin-3 (Gal-3), only Troponin I levels were higher. Directly after exercise, all cardiac biomarker levels were higher compared to the baseline, Gal-3 and BNP levels decreased 3 hours after completion of the run.Troponin values continued to increase with highest levels 3 hours after exercise. Troponin T, NT-Pro BNP and Gal-3 also showed significant correlation to markers of inflammation, fibrosis and renal function. Conclusions: Exercises of different intensity can be associated with biochemical abnormalities and longterm consequences are unknown. In chest pain patients presenting to the emergency department possible impact of exercise on test results, especially Troponin, should be checked. [less ▲]

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See detailPreserved wake-dependent cortical excitability dynamics predict cognitive fitness beyond age-related brain alterations.
Van Egroo, Maxime ULiege; Narbutas, Justinas ULiege; Chylinski, Daphné ULiege et al

in Communications Biology (2019), 2

Age-related cognitive decline arises from alterations in brain structure as well as in sleep-wake regulation. Here, we investigated whether preserved wake-dependent regulation of cortical function could ... [more ▼]

Age-related cognitive decline arises from alterations in brain structure as well as in sleep-wake regulation. Here, we investigated whether preserved wake-dependent regulation of cortical function could represent a positive factor for cognitive fitness in aging. We quantified cortical excitability dynamics during prolonged wakefulness as a sensitive marker of age-related alteration in sleep-wake regulation in 60 healthy older individuals (50-69 y; 42 women). Brain structural integrity was assessed with amyloid-beta- and tau-PET, and with MRI. Participants' cognition was investigated using an extensive neuropsychological task battery. We show that individuals with preserved wake-dependent cortical excitability dynamics exhibit better cognitive performance, particularly in the executive domain which is essential to successful cognitive aging. Critically, this association remained significant after accounting for brain structural integrity measures. Preserved dynamics of basic brain function during wakefulness could therefore be essential to cognitive fitness in aging, independently from age-related brain structural modifications that can ultimately lead to dementia. [less ▲]

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See detailRecertification of 25-hydroxyvitamin D standards by Isotope Pattern Deconvolution (IPD)
Fabregat Cabello, Neus ULiege; Pitarch-Motellón, Jorge; LE GOFF, Caroline ULiege et al

Scientific conference (2018, April 20)

Vitamin D (VTD) is an important prohormone widely known since its deficiency is directly related to development of rickets in children and osteoporosis in adults. Furthermore, recent studies have ... [more ▼]

Vitamin D (VTD) is an important prohormone widely known since its deficiency is directly related to development of rickets in children and osteoporosis in adults. Furthermore, recent studies have demonstrated that vitamin D has also an important role in non-skeletal conditions such as autoimmune diseases, cardiovascular diseases and cancer, among others. This vitamin can be found in two main forms: vitamin D2 and vitamin D3. The metabolism of both forms of vitamin D are subjected to a first hydroxylation in the liver to form 25-hydroxyvitamin D (25(OH)D) and then to a second one in the kidney to form 1,25-dihydroxyvitamin D (1,25(OH)2D), the active form of vitamin D. Nevertheless, the measurement of 25(OH)D in serum samples is preferred test for the assessment of vitamin D status over the 1,25(OH)2D. There are two main reasons for this choice: the longer lifetime (3 weeks versus 4 h) and its higher concentration levels (ng/mL versus pg/mL)[2]. Over the last years, a dramatic rise in vitamin D testing (as 25(OH)D) has been observed, due to two main reasons: the increased number of patients with VTD deficiency and the increase of the role of VTD as a biomarker related to several diseases. In this work we propose a recertification approach for 25(OH)D2/D3 solvent standards based on Isotope Pattern Deconvolution (IPD) using NIST SRM 2972 as reference material. This approach could help to meet the requirements for external standardization criteria using in-house calibration curves. [less ▲]

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See detailDosage des catécholamines et des métabolites par LC-MS/MS
LE GOFF, Caroline ULiege

Conference (2018, March 13)

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See detailLes biomarqueurs cardiaques: le tour de la question
LE GOFF, Caroline ULiege

Conference (2018, March 09)

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See detailComparison of cardiac biomarkers fluctuation in runners of marathons, semi-marathons and untrained runners
Le Goff, Caroline ULiege; VRANKEN, Laura ULiege; Kaux, Jean-François ULiege et al

in European Journal of Sports Medicine (2017, September), 5(Supplement 1), 16

BACKGROUND: Regular exercise like running is one important part of the prevention program of cardiovascular disease. There are several studies on biomarker changes during marathons especially cardiac ... [more ▼]

BACKGROUND: Regular exercise like running is one important part of the prevention program of cardiovascular disease. There are several studies on biomarker changes during marathons especially cardiac biomarkers have been studied and mild to moderate elevations have been described as a results of a running exercise Exact underlying mechanism for these biomarker elevations reflecting physiological or even pathobiological changes is unknown and less trained athletes might exhibit a higher risk compared to well trained The aim of our study was to compare three cardiac biomarkers for ischemic condition (Troponin), cardiac stretch (natriuretic peptides) and fibrotic processes (Galectin3) were tested in different type of runners, trained marathon and semimarathon runners and untrained runners before, directly after and 3 hours after the running exercise. METHODS: 23 (mean age 41± 8.8 yo) marathon runners, 15 semimarathon runners(44.1±8.4yo) and 17 healthy sedentary subjects (37± 4.4 yo) (race of 10 km, <2h of sport/week). Blood samples were taken just before (T0), just after (T1) and 3 hours (T3) after the race, centrifuged, aliquoted and stored frozen at 80C before further analysis. The study was approved by the Ethical Committee of our University Hospital. The analyses were performed on the Abbott ARCHITECT i2000SR (Abbott Laboratories, Germany) for the hs cTnI, BNP and Gal3 and on the C8000 (Roche Diagnostics, Switzerland) for hscTnT and NTproBNP according to the manufacturer’s instructions for use. RESULTS: In all 3 running groups there is an increase of cardiac biomarkers Troponin I, BNP, Galectin3 and NTProBNP after completion of the physical exercise. Biomarkers increase is depending on the intensity and duration of the exercise and is higher in long distance marathon and semimarathon runners compared to the control group with a 1 hour run. Cardiac biomarker levels between trained marathon and semimarathon runners were not statistically different in the preexercise baseline samples for BNP, NTProBNP and Galectin3. Compared to untrained runners only Troponin I levels were higher in baseline sample of marathon runners (hscTnI, p<0.03) when compared to controls, cardiac Troponin T (hscTnT, p<0.29) was less significant. CONCLUSIONS: Our study demonstrates that exercises of different intensity can be associated with biochemical abnormalities that may reflect adverse consequences on cardiac structure as fibrosis and biology. With exeption of Troponin I there was no difference in the preexercise baselines samples. [less ▲]

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See detailImpact of a mountain ultra-marathon (UTMB) on cardiac biomarkers
Le Goff, Caroline ULiege; Gergelé, Laurent; Kaux, Jean-François ULiege et al

in European Journal of Sports Medicine (2017, September), 5(Supplement 1), 15-16

OBJECTIVES: While moderate exercise has beneficial effects on the cardiovascular system, consequences of a supraphysiological effort are not clear yet. In particular, the physiological consequences of ... [more ▼]

OBJECTIVES: While moderate exercise has beneficial effects on the cardiovascular system, consequences of a supraphysiological effort are not clear yet. In particular, the physiological consequences of ultramarathons need to be further documented. The aim of the study was to assess the changes of various cardiac biomarkers after a mountain ultramarathon. MATERIAL AND METHODS: Blood and urine samples were collected on 28 runners (17 men) participating to the UltraTrail du Mont Blanc (105 km, total positive elevation: 5600 m) at 3 different times: before the race (Pre), within 1 h after the finish (Post) and 7 days after the finish (D+7). Several biomarkers involved in heart disease (coronary syndrome, heart failure and fibrosis) and in inflammation were assayed on different analyzers such a COBAS® (for CKMB,TnThs, NTproBNP, HFABP and CRPs) and KRYPTOR® (for Copeptin). ST2 was measured manually with the Presage kit from CRITICAL DIAGNOSTIC®. RESULTS: Plasma levels of cardiac markers (CKMB, TnThs, NTproBNP, copeptin, HFABP, ST2) and inflammation (CRPs) increased significantly at Post. Means values increased from Pre to Post as follows: 2.3 to 91.9 UI/L for CKMB (p<0.0001); 7.6 to 31.7 ng/L for TnThs (p<0.0001); 41.7 to 1190.5 ng/L for NTproBNP, 4.2 to 22.9 pmol/L for copeptin (p=0.001); 3.6 to 107.8 ng/mL for HFABP (p<0.0001), 29.7 to 126.2 ng/mL for ST2 (p<0.0001); 0.5 to 29.1 mg/L for CRPs (p<0.0001). With the exception of a few (HFABP, ST2, CRPs) biomarkers in some subjects, all values were back to Pre values at D+7. DISCUSSION/CONCLUSION: Prolonged strenuous running exercise caused an elevation in cardiac biomarkers. Elevation in CKMB levels lacks specificity for cardiac damage as runners have increased CKMB from skeletal muscles as well. Previous studies suggested that exercise induced TnThs elevation is a benign reversible physiologic phenomenon but this parameter, as well as HFABP, could be a sign of ischemia. Different phenomena occurred such as stretch of myocytes causing an increase in pressure or volume and neurohormonal activation which can explain the Copeptine and NTproBNP increase, while ST2 is a biomarker of cardiac remodeling and fibrosis. CRP is an acute phase compound that tends to increase following a strenuous and prolonged bout of exercise and/or muscular injury. As the values tended to return within the normal reference range values within 7 days after the race, our study suggests that there is no permanent structural damage at the myocardium level. [less ▲]

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See detailExploring the effect of a second closely-timed PRP infiltration for tendinopathy
Kaux, Jean-François ULiege; Forthomme, Bénédicte ULiege; Le Goff, Caroline ULiege et al

in European Journal of Sports Medicine (2017, September), 5(Supplement 1), 20-21

INTRODUCTION: Although PRP is very popular in sport, especially since it was removed from the doping list, it remains controversed in literature (3). Up to now, there exists no general agreement on the ... [more ▼]

INTRODUCTION: Although PRP is very popular in sport, especially since it was removed from the doping list, it remains controversed in literature (3). Up to now, there exists no general agreement on the preparation and the use of PRP. Some clinical series have previously evaluated the effect of PRP in the treatment of proximal patellar tendinopathies. Although it is possible that a single infiltrative administration may prove to be an effective treatment for this indication, most of the existing studies evaluated the effects of two or three successive infiltrations. The multiplication of infiltrations is arguably likely to increase the risks of complications, and this treatment can be expensive. PURPOSE: We aimed to evaluate whether two infiltrations of PRP prove more effective than a single treatment. METHODS: Our study is a single blinded, randomized controlled clinical trial on leisure sportsmen with chronic proximal patellar tendinopathies, rebel to classical management, including physiotherapy, shock wave therapy… Twenty patients suffering from proximal patellar tendinopathies for over than 3 months were enrolled into the study. PRP was obtained using an aphaeresis machine (1). The subjects were split into two randomized groups and beneficed of 1 or 2 infiltrations of pure PRP, respectively. The one-year followup evaluation consisted of VAS, IKDC and VISAP scores, while algometer, isokinetic and ultrasounds evaluations were carried out up to 3 months. RESULTS: The concentration of the PRP used for each infiltration was similar in both groups (913.20 ± 65.60 × 103/ L for group 1 and 917.90 ± 63.08for group 2, with virtually no red (<0.001 × 106/ L) nor white cells (<0.001 × 103/ L) in either group). The VAS significantly decreased with time over the 3 month followup period (p = 0002), with no difference observed between the two groups (p = 0.2). Values obtained with the pressure algometer increased with time across both groups over the 3 month followup period (p < 0.0001), and values were significantly higher for Group 1 (p = 0.001). The IKDC score increased with time in both groups over the followup period (p = 0.034), with values again significantly higher for Group 1 (p = 0.0026). The VISAP score increased with time in both groups over the followup period (p = 0.0023), with no difference observed between the groups (p = 0.41). No improvements in isokinetic physical performance were observed in either group. However, pain during E30 significantly decreased over the 3 month followup period (p = 0.027) for patients in both groups. No improvement in either jumping performances or in pain was observed in either group during optojump evaluation. No improvements in US findings were observed. However, an increase of the sagittal hypoechoic area was observed in Group1 (p = 0.0038). After one year, 90% patients of group 1 did not report anymore pain during daily activities, in comparison with only 20% in group 2. In group 1, 20% of subjects still described pain during work activities and 40% during practicing sports versus 40% and 70%, respectively, in group 2. One patient in each group did not return to sport; both subjects still experienced pain through daily and occupational work activities. Six subjects among the group 1 (67%) and 7 among the group 2 (78%) returned to their former sport, and 55% of both groups to the former level than before the tendinopathy. However, 44% of the group 1 and 78% of the group 2 still experienced pain during sports activities. The practiced sports were football, handball, cycling, running, fitness.On the other hand, patients with only few months of symptoms did not evolved more favorably than those with symptoms for longer. CONCLUSIONS: The comparison between 1 or 2 infiltrations of PRP did not reveal any difference between the 2 groups after a followup period of 3 months. A second closely timed infiltration of PRP to treat proximal patellar tendinopathies is not necessary to improve the efficacy of this treatment in the short term (2). However, there remains a need to evaluate the longer term results. REFERENCES: Kaux JF, Croisier JL, Bruyere O, Rodriguez De La Cruz C, Forthomme B, Brabant G, Lapraille S, Lonneux V, Noel D, Le Goff C, Gothot A, Collette J, Crielaard JM. One injection of plateletrich plasma associated to a submaximal eccentric protocol to treat chronic jumper's knee. J Sports Med Phys Fitness. 2015 Sep;55(9):95361 Kaux JF, Croisier JL, Forthomme B, Le Goff C, Buhler F, Savanier B, Delcour S, Gothot A, Crielaard JM. Using plateletrich plasma to treat jumper's knees: Exploring the effect of a second closelytimed infiltration. J Sci Med Sport. 2016 Mar;19(3):2004. Kaux JF, Drion P, Croisier JL, Crielaard JM. Tendinopathies and plateletrich plasma (PRP): from preclinical experiments to therapeutic use. J Stem Cells Regen Med. 2015 May 30;11(1):717. A10 [less ▲]

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See detailA fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS (powerpoint)
Fabregat Cabello, Neus ULiege; Farre Segura, Jordi ULiege; Huyghebaert, Loreen ULiege et al

Scientific conference (2017, September)

We present here a rapid, easy, reliable and cost-effective method for the quantification of 25-hydryxoyvitamin D2 and D3, epi-25-hydroxyyvitamin D3 and 24,25-dihydroxyvitamin D3 by LC-MS/MS in serum ... [more ▼]

We present here a rapid, easy, reliable and cost-effective method for the quantification of 25-hydryxoyvitamin D2 and D3, epi-25-hydroxyyvitamin D3 and 24,25-dihydroxyvitamin D3 by LC-MS/MS in serum samples. The proposed methodology has been strongly validated with both NIST and Labquality materials, obtaining mean intra-assay and inter-assay imprecision lower than 6 and 6.4% and mean recoveries within 95-104%. Besides we have compared satisfactorily samples from Vitamin D Standardization Program (n=80) with reference values and patient samples (n=281) with our reference LC-MS/MS method. The proposed methodology is prepared to be used in routine testing and permits the calculation of the Vitamin D Metabolite Ratio (VMR). [less ▲]

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See detailQuantification of serum androstanediol glucuronide
LE GOFF, Caroline ULiege

in Clinical Chemistry and Laboratory Medicine (2017, June)

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See detailEstablishment of reference values for 6 six steroids in serum
LE GOFF, Caroline ULiege; Fabregat Cabello, Neus ULiege; Huyghebaert, Loreen ULiege et al

in Clinical Chemistry and Laboratory Medicine (2017, June)

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