Publications of Caroline LE GOFF
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See detailThe impact of an ultra-trail on the dynamic of cardiac, inflammatory, renal and oxidative stress biological markers correlated with electrocardiogram and echocardiogram
LE GOFF, Caroline ULiege; Kaux, Jean-François ULiege; DULGHERU, Raluca Elena ULiege et al

in Acta Cardiologica (in press)

The aim of this study was to describe the effects of a 64.2 km ultra-trail on the biomarkers of muscle damage, inflammation and oxidative stress, and compare the results observed with an ECG and an ... [more ▼]

The aim of this study was to describe the effects of a 64.2 km ultra-trail on the biomarkers of muscle damage, inflammation and oxidative stress, and compare the results observed with an ECG and an echocardiogram, both performed before and after the race. Thirty-three ultra-trail volunteers (45.8 ± 8.7 years old) were enrolled in our study. Three blood tests were drawn from each runner, one just before (TPRE), one just after (TPOST) and the last 3 h after the end of the race (TPOST3h). All the markers increased. The maximum concentrations observed were at TPOST3h and were significant (p<0.001) for creatine kinase, creatine kinase isoform MB, high-sensitivity C-reactive protein, uric acid and for the ratio of reduced glutathione to oxidised glutathione. However, in the case of myoglobin, high-sensitive troponin T, N-terminal pro-brain natriuretic peptide, oxidised glutathione, myeloperoxidase, cystatin C and creatinine, the most significant increases were at TPOST (p<0.001). Modifications were observed in the medical imaging using echocardiography such as reduction of left ventricule end-sytolic and diastolic volumes and left ventricular global longitudinal strain. ECG showed electrical criteria for left ventricular hypertrophy and incomplete right bundle branch block after the race. Endurance races cause significant physiological stress to the body that can be measured by the increase of different biomarkers. From a laboratory perspective, it is important to take into account the possible exercise performed previous to the testing to avoid a misinterpretation of the results. From a training perspective, due to these increases in biomarkers, it is recommended that runners wait at least 72 h after an ultra-trail before subsequent training. In addition a transient impairment of ventricular function due to dehydration were observed. [less ▲]

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See detailIntense sport practices and cardiac biomarkers
LE GOFF, Caroline ULiege; Farre Segura, Jordi ULiege; Dufour, Patrice ULiege et al

in Clinical Biochemistry (2020), 79

Biomarkers are well established for the diagnosis of myocardial infarction, heart failure and cardiac fibrosis. Different papers on cardiac biomarker evolution during exercise have been published in the ... [more ▼]

Biomarkers are well established for the diagnosis of myocardial infarction, heart failure and cardiac fibrosis. Different papers on cardiac biomarker evolution during exercise have been published in the literature and generally show mild to moderate elevations. However, the mechanism responsible for these elevations, reflecting physiological or even pathophysiological changes, still has to be clearly elucidated. There are also indications of higher cardiac risk in poorly trained athletes than in well-trained athletes. Whether regular repetition of intensive exercise might lead, in the longer term, to fibrosis and heart failure remains to be determined. In this review, we summarized the main research about the effects of intense exercise (in particular, running) on cardiac biomarkers (including troponins, natriuretic peptides, etc.). We found that cardiac fibrosis biomarkers seemed to be the most informative regarding the biological impact of intense physical activity. [less ▲]

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See detailParathormone, bone alkaline phosphatase and 25-hydroxyvitamin D status in a large cohort of 1200 children and teenagers.
LADANG, Aurélie ULiege; ROUSSELLE, Olivier ULiege; Huyghebaert, Loreen ULiege et al

in Acta Clinica Belgica (2020)

OBJECTIVES: 25-Hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH) and bone alkaline phosphatase (BALP) are biomarkers of calcium/phosphate metabolism and bone turnover. Although vitamin D deficiency is ... [more ▼]

OBJECTIVES: 25-Hydroxyvitamin D (25(OH)D), parathyroid hormone (PTH) and bone alkaline phosphatase (BALP) are biomarkers of calcium/phosphate metabolism and bone turnover. Although vitamin D deficiency is a well-known cause of secondary hyperparathyroidism, few studies have considered vitamin D status when establishing reference ranges. In this study, we report PTH levels according to the vitamin D status and BALP levels in a large cohort of 1200 children. Additionally, we provide PTH pediatric reference values according to 25(OH)D status as well as BALP pediatric reference ranges. METHODS: Serum samples from 1200 children (equally distributed from 5 months to 20 years old) who underwent blood sampling for allergy exploration were used to quantify 25(OH)D, PTH and BALP. RESULTS: The percentage of vitamin D deficient children (<20 ng/ml) progressively increased during childhood starting from 7% in the 0 to 2 year-old subgroup to a mean of at least 50% among teenagers. PTH levels inversely mirrored 25(OH)D concentrations for all age and gender subgroups, and 25(OH)D deficient subgroups presented higher PTH levels than their non-deficient counterparts. In the non-deficient 25(OH)D population, PTH levels were the highest at 11 years old for girls and 14 years old for boys. BALP results were slightly increased during childhood and showed a constant decrease during teenage years starting from 12 years old for girls and 14 years old for boys. CONCLUSION: Our results highlight the inverse relationship between PTH and 25(OH)D in children and the need for a well characterized 25(OH)D population to establish pediatric reference ranges for PTH. [less ▲]

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See detailLe COVID-19 pourrait être dangereux pour le coeur des sportifs
ANCION, Arnaud ULiege; LE GOFF, Caroline ULiege; Kaux, Jean-François ULiege

Article for general public (2020)

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See detailThe pathway through LC-MS method development: in-house or ready-to-use kit-based methods?
LE GOFF, Caroline ULiege; Farre Segura, Jordi ULiege; STOJKOVIC, Violeta ULiege et al

in Clinical Chemistry and Laboratory Medicine (2020)

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See detailEvolution of the slopes of ST2 and galectin-3 during marathon and ultratrail running compared to a control group
LE GOFF, Caroline ULiege; Kaux, Jean-François ULiege; Farre Segura, Jordi ULiege et al

in Clinical Chemistry and Laboratory Medicine (2020), 58(2), 314-321

Background Previous studies have suggested that exercising may induce cardiac damage. Galectin-3 (Gal-3) and soluble suppression of tumorigenicity 2 (ST2) are very interesting biomarkers for heart failure ... [more ▼]

Background Previous studies have suggested that exercising may induce cardiac damage. Galectin-3 (Gal-3) and soluble suppression of tumorigenicity 2 (ST2) are very interesting biomarkers for heart failure and myocardial fibrosis. We aimed to compare the kinetics of emerging fibrosis cardiac biomarkers as Gal-3 and ST-2 in endurance runners, and recreational runners before and after a running event represented by a marathon and an ultratrail event. Methods Blood samples were taken from 19 healthy non-elite marathon runners (42 km), 27 ultratour runners (67 km), and 14 recreational runners who represented the control group (10 km) just before the run (T0), just after (T1) and 3 h after (T2), in order to analyze Gal-3, ST2, hsTnT, NT-proBNP, CKMB and hsCRP. We compared the percentage of evolution and the slopes obtained from T0 to T1 (pT0T1) and from T1 to T2 (pT1T2), between the different groups of runners participating in three different races. Results Plasma cardiac biomarker concentrations increased significantly from baseline to immediately post-exercise and most of the time decreased over the subsequent 3-h period. For pT0T1 and pT1T2, the markers Gal-3 and ST2 showed a significant difference between types of run (p < 0.05 and p < 0.0001, respectively). During the recovery time, Gal-3 returned to the baseline values but not ST2 which continued to increase. Conclusions Gal-3 and ST2 are considered as a reflection of cardiac fibrosis and remodeling. The evolution of both was different, particularly after the recovery time. ST2 values exceeding cutoff values at any time. [less ▲]

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See detailSimultaneous measurement of 25(OH)-vitamin D and 24,25(OH)2-vitamin D to define cut-offs for CYP24A1 mutation and vitamin D deficiency in a population of 1200 young subjects.
Cavalier, Etienne ULiege; Huyghebaert, Loreen ULiege; ROUSSELLE, Olivier ULiege et al

in Clinical chemistry and laboratory medicine (2020), 58(2), 197-201

Background Simultaneous measurement of 25(OH)D and 24,25(OH)2D is a new tool for predicting vitamin D deficiency and allows evaluating CYP24A1 lack of function. Interpretation of 24,25(OH)2D should be ... [more ▼]

Background Simultaneous measurement of 25(OH)D and 24,25(OH)2D is a new tool for predicting vitamin D deficiency and allows evaluating CYP24A1 lack of function. Interpretation of 24,25(OH)2D should be performed according to 25(OH)D levels and a ratio, called the vitamin D metabolite ratio (VMR) has been proposed for such a purpose. Unfortunately, the VMR can be expressed in different ways and cannot be used if 24,25(OH)2D concentrations are undetectable. Here, we propose evaluating the enzyme activity taking into consideration the probability that a normal population presents undetectable 24,25(OH)2D concentrations according to 25(OH)D levels. We thus retrospectively measured 25(OH)D and 24,25(OH)2D in a population of 1200 young subjects to evaluate the 25(OH)D threshold above which the enzyme was induced. Methods Serum samples from 1200 infants, children, adolescent and young adults were used to simultaneously quantify 25(OH)D and 24,25(OH)2D by LCMS/MS. Results Median (interquartile range [IQR]) levels were 20.6 (14.4-27.2) ng/mL for 25(OH)D. 172 subjects (14.3%) presented 24,25(OH)2D values below the LOQ. When 25(OH)D values were <11 ng/mL, 63.1% of subjects presented undetectable 24,25(OH)2D concentrations. Percentage decreased with increasing 25(OH)D values to become 19.7% for 25(OH)D comprised between 12 and 15 ng/mL, 5.1% for 25(OH)D between 16 and 20 and 0.7% for 25(OH)D >21 ng/mL. Conclusions We suggest using a statistical approach to evaluate CYP24A1 function according to 25(OH)D concentrations. Our results also show that vitamin D deficiency, as defined biochemically, could be around 20 ng/mL in infants, children, adolescent and young adults and that vitamin D deficiency could be evaluated on a more individual basis. [less ▲]

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See detailThe pathway through LC-MS method development: homemade or kit-based?
LE GOFF, Caroline ULiege

Conference (2019, December 17)

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See detailCardiac biomarkers and ultra-endurance
LE GOFF, Caroline ULiege

Conference (2019, November 29)

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See detailSerum Klotho Levels in ultra-trailers of the Tor des Géants
LE GOFF, Caroline ULiege

Poster (2019, November 15)

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See detailSERUM FREE CORTISOL QUANTIFICATION BY LC-MSMS
LE GOFF, Caroline ULiege; Nascimento, Helena; Disecare, Monica et al

Poster (2019, November 15)

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See detailImpact of Metformin on vitamin B12 status
VRANKEN, Laura ULiege; FONTAINE, Corentin ULiege; VALDES SOCIN, Hernan Gonzalo ULiege et al

Poster (2019, November 15)

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See detailEffort as a sensitive variable of wakefulness extension and its impact on cognitive performance
Mouraux, Charlotte; Van Egroo, Maxime ULiege; Chylinski, Daphné ULiege et al

Conference (2019, November)

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See detailMise au ppoint sur l'adrénarche prématurée: quel cut-off? Quelles méthodes?
LE GOFF, Caroline ULiege

Scientific conference (2019, October 24)

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See detailDetermination of iohexol by capillary blood microsampling and UHPLC-MS/MS
Ion, Valentin ULiege; Le Goff, Caroline ULiege; Cavalier, Etienne ULiege et al

in Journal of Pharmaceutical Analysis (2019), 9(4), 259-265

One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate (GFR ... [more ▼]

One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate (GFR). Iohexol is up to this moment a good candidate molecule for the GFR assessment since it exhibits minimum protein binding rates and minimum extra-renal clearance, being neither secreted nor reabsorbed at the tubular level. This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood, prelevated using volumetric absorbative microsampling (VAMS) systems. As an alternative to VAMS, a brand new HemaPEN® device for micro-prelevation was also tested. A new high throughput sample preparation protocol adapted for iohexol quantification from whole blood VAMS samples was developed. The medium term stability study of iohexol in dried whole blood VAMS samples that was conducted showed a good stability of this molecule for up to 12 days. By collecting only 10 μL of blood, iohexol can be analyzed from dried whole blood VAMS samples for concentration ranges between 1 and 250 μg/mL. Due to the analyte stability in VAMS for up to 12 days, this approach might be successfully applied for GFR assessment for clinical cases allowing minimum invasiveness and even delayed analysis. © 2019 Xi'an Jiaotong University [less ▲]

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See detailImpact of participating to a 330 km long ultra-trail versus a 67 km short ultra-trail on muscular and cardiac genetic regulation by microRNAs
Le Goff, Caroline ULiege; Marguerit, K; Kaux, Jean-François ULiege et al

in Bunc, V; Tsolakidis, E (Eds.) Book of abstracts - 24th Annual Congress of the European College of Sport Science (2019, July)

INTRODUCTION: Even though endurance exercise has always shown beneficial action on cardiovascular diseases, the impact of an ultra-endurance experience on the human body has not been studied enough yet ... [more ▼]

INTRODUCTION: Even though endurance exercise has always shown beneficial action on cardiovascular diseases, the impact of an ultra-endurance experience on the human body has not been studied enough yet. Indeed, the consequences of such effort could represent a major issue to the organism and particularly to the myocardial muscle, could lead to sudden cardiac death when experienced too many times - a phenomenon which is more and more linked to the athletic world. The main objective of this study was to measure the impact of high level physical activity on the expression of different microRNAs such as specific to muscular and cardiac suffering (miR-1, miR 133a and miR 208b), specific to muscular suffering (miR 133b, miR 206, miR 499a and miR 499b) specific to cardiac suffering (miR 208a) and specific to fibrosis (miR 21 and miR 29b). METHODS: The study was conducted on two different trails participants: the “Tor des Géants” (TDG), 330 km long and the “Ultra Tour de Liège” (UTL), 67km long. Blood samples were collected at different timings. Timing of sampling were respectively: 4 days before starting (t0), after completing 148 km (t2), finish line (t3) and 3 days after the end of the race for the TDG (t4) , and for the UTL : just before the starting (t0), at the finish line( t2) : and 3 hours after finishing (t3). There was a number of 12 TDG studied volunteers and 10 from the UTL. MicroRNAs were extracted by using miRneasy® Serum/Plasma kit and qRT-PCR has been employed to measure their expression. RESULTS: Only miR-1, miR-133a, miR-133b, miR-206 and miR-499a showed significant variation in their expression during both races. The variation was found to be linked to the exhaustion during the race. From these data, four out of the five miRNAs showed a higher expression level during the shorter trail (UTL). No correlation was found between miR-208a and frequently used cardiac biomarkers (hs-TnT, sST2 and NT-proBNP). No significant variation was found in fibrosis specific microRNAs (miR-21 and miR-29b). CONCLUSION: From the amount of microRNAs tested, the expression of five of them increased significantly during both the races, which showed myocardial and muscular cellular suffering. Moreover, the levels of miRNA never came back to the baseline during recovery times. However, the fibrotic miRNA did not show any significant increase during in any race, suggesting there was no permanent damage of the heart, but this point needs further investigations. [less ▲]

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