Publications of Jean DEFRAIGNE
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See detailCompared Phenolic Compound Contents of 22 Commercial Fruit and Vegetable Juices: Relationship to Ex-Vivo Vascular Reactivity and Potential In Vivo Projection
Matute Matute, Alexis Fernando ULiege; Tabart, Jessica ULiege; CHERAMY-BIEN, Jean-Paul ULiege et al

in Antioxidants (2020), 9(2), 92

The real impact of polyphenol-rich vegetable and fruit juice intake on cardiovascular health remains a matter of controversy. In the present study, rat aorta segments immersed in an organ bath (OB) were ... [more ▼]

The real impact of polyphenol-rich vegetable and fruit juice intake on cardiovascular health remains a matter of controversy. In the present study, rat aorta segments immersed in an organ bath (OB) were used to explore whether the total polyphenol content and/or individual phenolic compound contents of 22 commercial vegetable (n = 3) and fruit juices [(citrus (n = 5), berries (n = 10), apple (n = 2), pineapple (n = 2)] might be associated with vascular tone. Red juices (particularly blackcurrant) and lemon juice caused the most marked vasorelaxation, its amplitude being endothelium dependent or not according to the volume ratio of juice to initial OB solution Vjuice/VOBS). At volume ratios 5% and 10%, both the juice and OB total polyphenol for all juices and total anthocyanin contents for berry juices significantly correlated with aorta vasorelaxation intensity. This was not the case for total or individual flavonols (except kaempferol) or for total or individual flavanols (except epigallocatechin gallate). If one relates our measured concentrations of individual phenolic compounds in OB to what is known about their physiological concentrations, and given our evidenced correlations between compound concentrations and vasorelaxation intensity, kaempferol, epigallocatechin gallate and peonidin-3-O-glucoside seem to emerge as the interesting phenolic compounds likely to be responsible for the potent vasorelaxation observed with fruit juices, and more particularly blackcurrant ones. Clinical investigation is required, however, to confirm our observations. [less ▲]

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See detailLa transplantation cardiaque : indications actuelles et resultats de l'experience liegeoise.
BRULS, Samuel ULiege; Tchana-Sato, Vincent ULiege; LAVIGNE, Jean-Paul ULiege et al

in Revue medicale de Liege (2020), 75(1), 29-36

Heart transplantation remains undoubtedly the most effective treatment for end-stage heart failure, whatever its cause. Last decade has witnessed significant improvements in terms of morbidity and ... [more ▼]

Heart transplantation remains undoubtedly the most effective treatment for end-stage heart failure, whatever its cause. Last decade has witnessed significant improvements in terms of morbidity and mortality following heart transplant. The 5-year survival rate is now beyond 70 %. However, the shortage of potential donors limits its use and requires strict criteria before listing a candidate for heart transplantation. Herein, we present a review of current indications and results of the heart transplantation program at the University hospital of Liege. [less ▲]

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See detailFirst report of a successful pediatric heart transplantation from donation after circulatory death with distant procurement using normothermic regional perfusion and cold storage.
Tchana-Sato, Vincent ULiege; Ledoux, Didier ULiege; Vandendriessche, Katrien et al

in The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation (2019), 38(10), 1112-1115

Heart transplantation (HT) from donation after circulatory death (DCD) is a promising alternative to expand the heart donor pool. Cold storage can be used in a strategy to successfully retrieve and ... [more ▼]

Heart transplantation (HT) from donation after circulatory death (DCD) is a promising alternative to expand the heart donor pool. Cold storage can be used in a strategy to successfully retrieve and transplant DCD hearts after reconditioning using normothermic regional perfusion for distant procurement. Herein, we present the first report of a pediatric DCD heart reconditioned with normothermic regional perfusion, preserved using only cold storage while being transported to a neighboring center, and then successfully transplanted after nearly 2 hours of cold static storage. If supported by an appropriate trial, this finding could obviate the need to use expensive perfusion devices for short interhospital distances for DCD heart transportation and stimulate more centers across the world to embrace DCD HT. [less ▲]

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See detailMechanical strain induces a pro-fibrotic phenotype in human mitral valvular interstitial cells through RhoC/ROCK/MRTF-A and Erk1/2 signaling pathways
Blomme, Benoit ULiege; Deroanne, Christophe ULiege; Hulin, Alexia ULiege et al

in Journal of Molecular and Cellular Cardiology (2019), 135

The mitral valve is a complex multilayered structure populated by fibroblast-like cells, valvular interstitial cells (VIC) which are embedded in an extracellular matrix (ECM) scaffold and are submitted to ... [more ▼]

The mitral valve is a complex multilayered structure populated by fibroblast-like cells, valvular interstitial cells (VIC) which are embedded in an extracellular matrix (ECM) scaffold and are submitted to the mechanical deformations affecting valve at each heartbeat, for an average of 40 million times per year. Myxomatous mitral valve (MMV) is the most frequent heart valve disease characterized by disruption of several valvular structures due to alterations of their ECM preventing the complete closure of the valve resulting in symptoms of prolapse and regurgitation. VIC and their ECM exhibit reciprocal dynamic processes between the mechanical signals issued from the ECM and the modulation of VIC phenotype responsible for ECM homeostasis of the valve. Abnormal perception and responsiveness of VIC to mechanical stress may induce an inappropriate adaptative remodeling of the valve progressively leading to MMV. To investigate the response of human VIC to mechanical strain and identify the molecular mechanisms of mechano-transduction in these cells, a cyclic equibiaxial elongation of 14% at the cardiac frequency of 1.16 Hz was applied to VIC by using a Flexercell-4000 T™ apparatus for increasing time (from 1 h to 8 h). We showed that cyclic stretch induces an early (1 h) and transient over-expression of TGFβ2 and αSMA. CTGF, a profibrotic growth factor promoting the synthesis of ECM components, was strongly induced after 1 and 2 h of stretching and still upregulated at 8 h. The mechanical stress-induced CTGF up-regulation was dependent on RhoC, but not RhoA, as demonstrated by siRNA-mediated silencing approaches, and further supported by evidencing RhoC activation upon cell stretching and suppression of cell response by pharmacological inhibition of the effector ROCK1/2. It was also dependent on the MEK/Erk1/2 pathway which was activated by mechanical stress independently of RhoC and ROCK. Finally, mechanical stretching induced the nuclear translocation of myocardin related transcription factor-A (MRTF-A) which forms a transcriptional complex with SRF to promote the expression of target genes, notably CTGF. Treatment of stretched cultures with inhibitors of the identified pathways (ROCK1/2, MEK/Erk1/2, MRTF-A translocation) blocked CTGF overexpression and abrogated the increased MRTF-A nuclear translocation. CTGF is up-regulated in many pathological processes involving mechanically challenged organs, promotes ECM accumulation and is considered as a hallmark of fibrotic diseases. Pharmacological targeting of MRTF-A by newly developed inhibitors may represent a relevant therapy for MMV. © 2019 [less ▲]

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See detailSuccessful clinical transplantation of hearts donated after circulatory death using normothermic regional perfusion
TCHANA-SATO, Vincent ULiege; LEDOUX, Didier ULiege; DETRY, Olivier ULiege et al

in Journal of Heart and Lung Transplantation (2019), 38(6), 593-598

BACKGROUND: Heart transplantation (HT) from donation after circulatory death (DCD) has yet to achieve wide clinical application despite the encouraging resultsreported recently. In this study we describe ... [more ▼]

BACKGROUND: Heart transplantation (HT) from donation after circulatory death (DCD) has yet to achieve wide clinical application despite the encouraging resultsreported recently. In this study we describe 2 cases of successful adult DCD HT performed at our institution using an original protocol. METHODS: Our local abdominal DCD protocol was updated to allow DCD heart procurement, and was accepted by the institutional ethics committee. The main features of the protocol include: pre-mortem insertion of peripheral venoarterial extracorporeal membrane oxygenation cannulas; thoracoabdominal normothermic regional perfusion (NRP) by clamping the 3 aortic arch vessels to exclude cerebral circu- lation; and in-situ heart resuscitation. The retrieved hearts were directly transplanted into recipients located in an adjoining operating room. RESULTS: The procurement warm ischemic time was 25 minutes for the first donor, and 26 minutes for the second donor. The cold ischemic time was 16 minutes for the first recipient and 17 minutes for the second recipient. The suture time was 30 minutes for the first recipient, and 53 minutes for the second recipient. Both recipients were easily weaned off cardiopulmonary bypass in sinus rhythm and inotro- pic support. Post-operative evaluation of cardiac function was excellent and the patients were subse- quently discharged home. CONCLUSIONS: Transplantation of hearts from DCD donors is now a clinical reality.NRP is a useful tool for resuscitation, reperfusion, and preservation of transplanted hearts. It also offers the opportunity to assess the function and viability of organs before transplantation. However,due to ethical issues, some may object to ante-mortem intervention. [less ▲]

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See detailHeart donation after circulatory death
LEDOUX, Didier ULiege; MASSION, Paul ULiege; HANS, Grégory ULiege et al

Conference (2019, March 14)

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See detailExtracorporeal CO2 removal and regional citrate anticoagulation in an experimental model of hypercapnic acidosis.
Morimont, Philippe ULiege; Habran, Simon; Desaive, Thomas ULiege et al

in Artificial Organs (2019)

Low flow extracorporeal veno-venous CO2 removal (ECCO2 R) therapy is used to remove CO2 while reducing ventilation intensity. However, the use of this technique is limited because efficiency of CO2 ... [more ▼]

Low flow extracorporeal veno-venous CO2 removal (ECCO2 R) therapy is used to remove CO2 while reducing ventilation intensity. However, the use of this technique is limited because efficiency of CO2 removal and potential beneficial effects on pulmonary hemodynamics are not precisely established. Moreover, this technique requires anticoagulation that may induce severe complications in critically ill patients. This study aimed at determining precisely efficiency of CO2 extraction and its effects on right ventricular (RV) afterload, and comparing regional anticoagulation with citrate to systemic heparin anticoagulation during ECCO2 R. ECCO2 R was highly efficient to normalize pH and PaCO2 and to reduce RV afterload resulting from hypercapnic acidosis. Regional anticoagulation with citrate solution was as effective as standard heparin anticoagulation but did not improve CO2 removal and lead to more hypocalcemia and hypotension. [less ▲]

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See detailEx-vivo vasorelaxation induced by polyphenol-rich extracts from plant, fruits and berries on porcine coronary artery rings
Ebabe Elle, Etienne ULiege; Defraigne, Jean-Olivier ULiege; Schini-Kerth, Valérie et al

in Free Radical Biology and Medicine (2018, June), 120

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See detailAnalyse détaillée de la seconde version de l’avant-projet de Code du bien-être animal wallon. Lecture commentée au 21/03/2018 du Chapitre 8 (Expérimentation animale)
Drion, Pierre ULiege; Corhay, Albert ULiege; Haubruge, Eric ULiege et al

Report (2018)

La compétence « bien-être animal », auparavant fédérale, a été régionalisée en juillet 2014. Ce projet de code vise à remplacer les dispositions légales en vigueur (la Loi de 1984 telle que modifiée par ... [more ▼]

La compétence « bien-être animal », auparavant fédérale, a été régionalisée en juillet 2014. Ce projet de code vise à remplacer les dispositions légales en vigueur (la Loi de 1984 telle que modifiée par les décrets du Gouvernement wallon). Certains éléments sont repris tels quels de la Directive 2010/63. Cela est nécessaire car la Directive européenne en tant que telle n’a pas de force obligatoire en Belgique. Elle doit être transcrite par un instrument législatif (avant, la Loi de 1984 et ses modifications, aujourd’hui, le projet de code pour la Région wallonne). Certains aspects semblent flous, mais renvoient à des dispositions que le Gouvernement doit encore prendre (au travers d’arrêtés du Gouvernement wallon, comme le faisaient avant les nombreux arrêtés royaux et du gouvernement qui réglementent la matière). Les arrêtés d’exécution devront obligatoirement tenir compte de la Directive européenne et s’inspirer de dispositions actuellement en vigueur. [less ▲]

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See detailWhat is the risk of cancer transmission with heart transplantation
Stiennon, Laurie ULiege; TCHANA-SATO, Vincent ULiege; ANCION, Arnaud ULiege et al

Poster (2018, March 16)

The organ donor shortage is particularly severe for heart grafts. Donor history of malignancy is a contra-indication for organ donation, but as the heart is an organ at lower risk of metastasis compared ... [more ▼]

The organ donor shortage is particularly severe for heart grafts. Donor history of malignancy is a contra-indication for organ donation, but as the heart is an organ at lower risk of metastasis compared to the liver or the lungs, the authors aimed to better determine this risk with heart transplantation (HTx) through an extensive review of the literature. Results: 7 cases (melanoma, glioblastoma (2), renal carcinoma, choriocarcinoma, hypernephroma, prostate) of transmission of cancer with HTx were reported. All recipients died from transmitted cancer. Interestingly, in 9 other cases, HTx did not induce cancer transmission at long term, despite the evidence of active cancer in the donor proven by autopsy or by cancer transmission in recipients of other organs from the same donor. Reports of UNOS described 274 heart donors with history of various cancers, without transmission to the recipients. In another report, UNOS reported 212 HTx from donor with brain cancer without cancer transmission to the recipients. Conclusion: HTx is not protected from the risk of donor cancer transmission, but very few cases were reported so far. This fact might indicate that donors with past history of cancer might be considered for heart donation in selected cases, considering the actual organ donor shortage. [less ▲]

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See detailAfterload burden on the right ventricle is enhanced when ARDS is associated with hypercapnic acidosis.
MORIMONT, Philippe ULiege; Habran, Simon ULiege; Desaive, Thomas ULiege et al

in Annals of Intensive Care (2018, February 05), 8(1),

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See detailLes urgences cardiaques et vasculaires : du diagnostic au traitement.
Scheen, André ULiege; Lancellotti, Patrizio ULiege; Defraigne, Jean-Olivier ULiege et al

in Revue Médicale de Liège (2018), 73(5-6), 221-223

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See detailLa dissection aortique
TCHANA-SATO, Vincent ULiege; SAKALIHASAN, Natzi ULiege; DEFRAIGNE, Jean ULiege

in Revue Médicale de Liège (2018), 73(5-6), 290-295

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See detailL’anévrisme rompu de l’aorte abdominale
TCHANA-SATO, Vincent ULiege; SAKALIHASAN, Natzi ULiege; DEFRAIGNE, Jean ULiege

in Revue Médicale de Liège (2018), 73(5-6), 296-299

Detailed reference viewed: 52 (7 ULiège)