Article (Scientific journals)
The application of reporter gene assays for the detection of endocrine disruptors in sport supplements
Plotan, Monica; Elliott, Chris; Scippo, Marie-Louise et al.
2011In Analytica Chimica Acta, 700, p. 34-40
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Keywords :
Endocrine disruptor; Estrogen; Androgen; Bioassay; Health food supplement; Food safety
Abstract :
[en] The increasing availability and use of sports supplements is of concern as highlighted by a number of studies reporting endocrine disruptor contamination in such products. The health food supplement market, including sport supplements, is growing across the Developed World. Therefore, the need to ensure the quality and safety of sport supplements for the consumer is essential. <br />The development and validation of two reporter gene assays coupled with solid phase sample preparation enabling the detection of estrogenic and androgenic constituents in sport supplements is reported. Both assays were shown to be of high sensitivity with the estrogen and androgen reporter gene assays having an EC50 of 0.01 ng mL-1 and 0.16 ng mL-1 respectively. <br />The developed assays were applied in a survey of 63 sport supplements samples obtained across the Island of Ireland with an additional seven reference samples previously investigated using LC-MS/MS. Androgen and estrogen bio-activity was found in 71 % of the investigated samples. Bio-activity profiling was further broken down into agonists, partial agonists and antagonists. Supplements (13) with the strongest estrogenic bio-activity were chosen for further investigation. LC-MS/MS analysis of these samples determined the presence of phytoestrogens in seven of them. Supplements (38) with androgen bio-activity were also selected for further investigation. Androgen agonist bio-activity was detected in 12 supplements, antagonistic bio-activity was detected in 16 and partial antagonistic bio-activity was detected in 10. A further group of supplements (7) did not present androgenic bio-activity when tested alone but enhanced the androgenic agonist bio-activity of dihydrotestosterone when combined. <br />The developed assays offer advantages in detection of known, unknown and low-level mixtures of endocrine disruptors over existing analytical screening techniques. For the detection and identification of constituent hormonally active compounds the combination of biological and physio-chemical techniques is optimal.
Research center :
Giga-Development and Stem Cells - ULiège
Disciplines :
Food science
Author, co-author :
Plotan, Monica
Elliott, Chris
Scippo, Marie-Louise  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Analyse des denrées alimentaires
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Antignac, Jean-Philippe
Malone, Edward
Bovee, Toine
Mitchell, Samuel
Connolly
Language :
English
Title :
The application of reporter gene assays for the detection of endocrine disruptors in sport supplements
Publication date :
2011
Journal title :
Analytica Chimica Acta
ISSN :
0003-2670
eISSN :
1873-4324
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
700
Pages :
34-40
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 January 2011

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