Article (Scientific journals)
Presence of Oestrogen Receptor Type Beta in Human Retina
Munaut, Carine; Lambert, Vincent; Noël, Agnès et al.
2001In British Journal of Ophthalmology, 85 (7), p. 877-82
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Keywords :
Aged; Blotting, Western/methods; Immunohistochemistry/methods; Male; Middle Aged; oestrogen receptor; ocular; RNA, Messenger/analysis; Receptors, Estrogen/analysis/genetics; Retina/chemistry; Retinal Ganglion Cells/chemistry; Reverse Transcriptase Polymerase Chain Reaction
Abstract :
[en] BACKGROUND/AIMS: Recent studies have demonstrated the existence of two oestrogen receptor subtypes alpha (ORalpha) and beta (ORbeta) with significant differences of expression among organs. Since important pathologies of human eye could be linked to hormonal status, the expression of ORbeta in ocular posterior segment was sought. METHODS: Immunohistochemical localisation of ORbeta and ORalpha protein and detection of OR mRNAs by reverse transcription-polymerase chain reaction (RT-PCR) were performed in macular and extramacular regions of the retina and in the choroid on male and female donors eyes. RESULTS: ORbeta protein was localised in the ganglion cell layer and in the choroid. At the transcriptional level, mRNA for ORbeta and for ORalpha were both present. Local differences in the expression level were observed, however, suggesting the possibility of variation in the ratio of ORalpha v ORbeta. CONCLUSIONS: The coexistence of two oestrogen receptor subtypes in the human ocular posterior segment raises acute questions about their potential physiological role, but offers a perspective for preferential targeting of a specific receptor subtype.
Disciplines :
Biochemistry, biophysics & molecular biology
Ophthalmology
Author, co-author :
Munaut, Carine  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Lambert, Vincent  ;  Centre Hospitalier Universitaire de Liège - CHU > Ophtalmologie
Noël, Agnès ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Frankenne, Francis 
Deprez, Manuel ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Rakic, Jean-Marie ;  Centre Hospitalier Universitaire de Liège - CHU > Ophtalmologie
Language :
English
Title :
Presence of Oestrogen Receptor Type Beta in Human Retina
Publication date :
2001
Journal title :
British Journal of Ophthalmology
ISSN :
0007-1161
eISSN :
1468-2079
Publisher :
British Medical Association, London, United Kingdom
Volume :
85
Issue :
7
Pages :
877-82
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 January 2009

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