Group B streptococcus; Intrapartum screening; Revogene
Résumé :
[en] Prevention of perinatal Group B Streptococcus (GBS) transmission is crucial in our effort to prevent Earlyonset GBS disease. Here, we established the performance of the Revogene GBS DS assay for the detection of group B streptococcus on intrapartum vaginal samples in a laboratory environment using a prospective noninterventional study design. Intrapartum vaginal swabs were enriched using a selective culture method which served as study reference method. Overall, 119 patients were enrolled with an antenatal and intrapartum Group B Streptococcus colonization prevalence of 12.9% and 11.8%, respectively. Compared to intrapartum culture, the Revogene GBS DS assay had a sensitivity of 92.9% and a specificity of 99.1%, while the antenatal culture displayed a sensitivity 78.6% of and specificity of 96.2%. The Revogene GBS DS assay displayed an acceptable performance according to the European Group B Streptococcus consensus recommendations.
Complementary studies in clinical practice are needed to confirm these findings.
Disciplines :
Médecine de laboratoire & technologie médicale
Auteur, co-auteur :
MEEX, Cécile ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire bactériologie
DEVEY, Anaïs ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service d'hématologie biologique et immuno-hématologie
Defêche, Justine ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Pool assistant - biologie clinique
BONTEMS, Sébastien ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire LRS
DESCY, Julie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire mycobactérie
HAYETTE, Marie-Pierre ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service de microbiologie clinique
SACHELI, Rosalie ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire CNR
MELIN, Pierrette ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service de microbiologie clinique
Langue du document :
Anglais
Titre :
Evaluation of the Revogene GBS DS assay performance for the intrapartum screening of group B streptococcus in comparison with intrapartum culture
American College of Obstetricians (ACOG). Prevention of Group B Streptococcal early-onset disease in newborns: ACOG committee opinion, number 797. Obstet Gynecol 135:2 (2020), e51–e72, 10.1097/aog.0000000000003335.
Belgian Superior Health Council. Prevention of perinatal Group B Streptococcal infections. Guidelines from the Belgian Health Council. 2003;1–26.
Bianchi-Jassir, F., Seale, A.C., Kohli-Lynch, M., Lawn, J.E., Baker, C.J., Bartlett, L., et al. Preterm birth associated with Group B Streptococcus maternal colonization worldwide: systematic review and meta-analyses. Clin Infect Dis 65:2 (2017), S133–S142, 10.1093/cid/cix661.
Buchan, B.W., Faron, M.L., Fuller, D., Davis, T.E., Mayne, D., Ledeboer, N.A., Multicenter clinical evaluation of the Xpert GBS LB assay for detection of Group B Streptococcus in prenatal screening specimens. J Clin Microbiol 53:2 (2015), 443–448 https://dx.doi.org/10.1128/2FJCM.02598-14.
Choera, T., Jung-Hynes, B., Chen, D.J., Comparative study of Revogene GBS LB assay and GeneXpert GBS LB assay for the detection of Group B Streptococcus in prenatal screening samples. BMC Infect Dis, 20(1), 2020, 38, 10.1186/s12879-019-4756-y.
Cools, P., Melin, P, Group B Streptococcus and perinatal mortality. Res Microbiol 168:9–10 (2017), 793–801, 10.1016/j.resmic.2017.04.002.
Di Renzo, G.C., Melin, P., Berardi, A., Blennow, M., Carbonell-Estrany, X., Donzelli, G.P., et al. Intrapartum GBS screening and antibiotic prophylaxis: a European consensus conference. J Matern Fetal Neonatal Med 53:2 (2015), 443–448, 10.3109/14767058.2014.934804.
El Helali, N., Giovangrandi, Y., Guyot, K., Chevet, K., Gutmann, L., Durand-Zaleski, I., Cost and Effectiveness of intrapartum Group B Streptococcus polymerase chain reaction screening for term deliveries. Obstet Gynecol 119:4 (2012), 822–829, 10.1097/aog.0b013e31824b1461.
El Helali, N., Nguyen, J.C., Ly, A., Giovangrandi, Y., Trinquart, L., Diagnostic accuracy of a rapid real-time polymerase chain reaction assay for universal intrapartum Group B Streptococcus Screening. Clin Infect Dis 49 (2009), 417–423, 10.1086/600303.
Gori, A., Harrison, O.B., Mlia, E., Nishihara, Y., Chan, J.M., Msefula, J., et al. Pan-GWAS of Streptococcus agalactiae highlights lineage-specific genes associated with virulence and niche adaptation. MBio, 11(3), 2020, 10.1128/MBIO.00728-20 e00728–20.
Horváth-Puhó, E., van Kassel, M.N., Gonçalves, B.P., de Gier, B., Procter, S.R., Paul, P., et al. Mortality, neurodevelopmental impairments, and economic outcomes after invasive Group B Streptococcal disease in early infancy in Denmark and the Netherlands: a national matched cohort study. Lancet Child Adolesc Health 5:6 (2021), 398–407, 10.1016/S2352-4642(21)00022-5.
Koebnick, C., Sidell, M.A., Getahun, D., Tartof, S.Y., Rozema, E., Taylor, B., et al. Intrapartum antibiotic exposure and body mass index in children. Clin Infect Dis, 2021, 10.1093/cid/ciab053 ciab053.
Madrid, L., Seale, A.C., Kohli-Lynch, M., Edmond, K.M., Lawn, J.E., Heath, P.T., et al. Infant Group B Streptococcal disease incidence and serotypes worldwide: systematic review and meta-analyses. Clin Infect Dis 65:2 (2017), S160–S172, 10.1093/cid/cix656.
Manning, D.S., Lewis, M.A., Springman, A.C., Lehotzky, E., Whittam, T.S., Davies, D., Genotypic diversity and serotype distribution of Group B Streptococcus isolated from women before and after delivery. Clin Infect Dis 46:12 (2008), 1829–1837, 10.1086/588296.
Mueller, M., Henle, A., Droz, S., Kind, A.B., Rohner, S., Baumann, M., et al. Intrapartum detection of Group B Streptococci colonization by rapid PCR-test on labor ward. Eur J Obstet Gynecol Reprod Biol 176 (2014), 137–141, 10.1016/j.ejogrb.2014.02.039.
Plainvert, C., El Alaoui, F., Tazi, A., Joubrel, C., Anselem, O., Ballon, M., et al. Intrapartum Group B Streptococcus screening in the labor ward by Xpert® GBS real-time PCR. Eur J Clin Microbiol Infect Dis 37:2 (2018), 265–270, 10.1007/s10096-017-3125-2.
Schrag, S.J., Zell, E.R., Lynfield, R., Roome, A., Arnold, K.E., Craig, A.S., et al. A population-based comparison of strategies to prevent early-onset Group B Streptococcal disease in neonates. N Engl J Med 347 (2002), 233–239, 10.1056/NEJMoa020205.
Schrag, S.J., Zywicki, S., Farley, M.M., Reingold, A.L., Harrison, L.H., Lefkowitz, L.B., et al. Group B Streptococcal disease in the era of intrapartum antibiotic prophylaxis. N Engl J Med 342 (2000), 15–20, 10.1056/NEJM200001063420103.
Seale, A.C., Bianchi-Jassir, F., Russell, N.J., Kohli-Lynch, M., Tann, C.J., Hall, J., et al. Estimates of the burden of Group B Streptococcal disease worldwide for pregnant women, stillbirths, and children. Clin Infect Dis 65:2 (2017), S200–S219, 10.1093/cid/cix664.
Seale, A.C., Blencowe, H., Bianchi-Jassir, F., Embleton, N., Bassat, Q., Ordi, J., et al. Stillbirth with Group B Streptococcus disease worldwide: systematic review and meta-analyses. Clin Infect Dis 65:2 (2017), S125–S132, 10.1093/cid/cix585.
Shin, J.H., Pride, D.T., Comparison of three nucleic acid amplification tests and culture for detection of Group B Streptococcus from enrichment broth. J Clin Microbiol 57:6 (2019), 1–9, 10.1128/jcm.01958-18.
Teatero, S., Ferrieri, P., Martin, I., Demczuk, W., McGeer, A., Fittipaldi, N., Serotype distribution, population structure, and antimicrobial resistance of Group B Streptococcus strains recovered from colonized pregnant women. J Clin Microbiol 55:2 (2017), 412–422, 10.1128/jcm.01615-16.
Tickler, I.A., Tenover, F.C., Dewell, S., Le, V.M., Blackman, R.N., Goering, R.V., et al. Streptococcus agalactiae strains with chromosomal deletions evade detection with molecular methods. J clin Microbiol, 57(4), 2019, 10.1128/jcm.02040-18 e02040-18.
Uzan-Yulzari, A., Turta, O., Belogolovski, A., Ziv, O., Kunz, C., Perschbacher, S., et al. Neonatal antibiotic exposure impairs child growth during the first six years of life by pertubing intestinal microbial colonization. Nat Commun, 12, 2021, 443, 10.1038/s41467-020-20495-4.
Verani, J.R., McGee, L., Schrag, S.J., Division of Bacterial Diseases, National Center for Immunization and Respiratory Diseases (CDC). Prevention of perinatal Group B Streptococcal disease: revised Guidelines from CDC. MMWR 59:RR–10 (2010), 1–36 2010.