[en] In all mammalian females, follicular growth and maturation are essentially dependent on the pituitary gonadotropins, FSH and LH. These glycoprotein hormones have many similarities, but their action, based on high affinity binding to specific membrane receptors, are quite different. The purpose of this study was to perform a sensitive localization of FSH and LH in secretory granules of gonadotrophs using highly specific antisera. This morphological study included light microscopy (PAP) and electron microscopy (immunogold single and double labeling) procedures. Histologically, approximatively 11.5% of cells were positive for LH, whereas only 5.4% of cells were positive for FSH. With the electron microscope, single labeling allowed identification of morphologically distinct LH-containing cells and FSH-containing cells. Double immunostaining confirmed that no cells contained both hormones. The finding that FSH and LH are produced in separate pituitary cells is in agreement with recent studies that have suggested a specific role and regulatory process for gonadotropins in the bovine species.
Centre de recherche :
Laboratoire d'Endocrinologie et de Physiologie de la Reproduction animale
Disciplines :
Endocrinologie, métabolisme & nutrition
Auteur, co-auteur :
Bastings, E.
Beckers, Albert ; Université de Liège - ULiège > Département des sciences cliniques > Endocrinologie
Reznik, Michel ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Langue du document :
Anglais
Titre :
Immunocytochemical evidence for production of luteinizing hormone and follicle-stimulating hormone in separate cells in the bovine.
Date de publication/diffusion :
1991
Titre du périodique :
Biology of Reproduction
ISSN :
0006-3363
eISSN :
1529-7268
Maison d'édition :
Society for the Study of Reproduction, Madison, Etats-Unis - Wisconsin