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See detailOur soil map as cultural heritage: what of the Belgium soil survey project should be preserved and what is being lost?
Legrain, Xavier ULiege; Dondeyne, Stefaan; Deckers, Jozef et al

Poster (2012, July 06)

Between 1947 and 1991, soils of Belgium were mapped to establish a systematic inventory of the country soil resources. Field observations were done by soil auger to a standard depth of 1.25 m and at a ... [more ▼]

Between 1947 and 1991, soils of Belgium were mapped to establish a systematic inventory of the country soil resources. Field observations were done by soil auger to a standard depth of 1.25 m and at a mean density of 2 points per hectare. Cadastral plans at scale 1:5,000 where used for georeferencing field observations and for delimiting map units, subsequently generalized on the 1:10,000 topographic base map. The final map was published on sheets at scale 1:20,000 along with descriptive texts. Besides, data on about 15,000 described and analyzed soil profiles were reported in technical annexes. With the advent of computers, data on soil profiles have been transfered into relational databases and soil sheets have been digitized. Coding of the data rendered them more accessible, but inevitably implied a standardization and hence a reduction of some information. Still most of the soil surveyors have already passed away, besides their intangible expert knowledge, a wealth of information is also being lost when their field notes, unpublished reports, minutes of meetings and draft maps are being disregarded. The map legend was developed during the first decade of the survey, reflecting state of knowledge on soil formation and their relative importance for agricultural land-use in the 1950s. To guarantee that future generations will be able to appreciate the value and concepts underpinning the soil information, it is important that at least a minimum set of such historical documents would be preserved, analyzed and documented. [less ▲]

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See detailMultiscale modelling of the influence of VEGF on sprouting angiogenesis.
Carlier, Aurélie ULiege; Geris, Liesbet ULiege; Van Oosterwyck, Hans

Poster (2012, July 06)

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See detailBehavioural effects of approach-avoidance motivational conflicts in Zebrafish: testing an Attentional Control Model on videotracked swimming activity
Ylieff, Marc ULiege; Froidbise, Sophie; Jacquet, Laurie et al

Poster (2012, July 05)

Motivational conflicts have been thoroughly studied in birds and mammals over the last decades, but their investigation has remained anecdotic with respect to fish. However, recent researches reveal that ... [more ▼]

Motivational conflicts have been thoroughly studied in birds and mammals over the last decades, but their investigation has remained anecdotic with respect to fish. However, recent researches reveal that, emotion and cognition also play a pivotal role in the expression of fish behaviour. Fish exhibit fear, long-term memory, attentional and learning capacities that are comparable with those of other vertebrates, including nonhuman primates. Thus, fish can be expected to manage motivational conflicts using cognitive similar resources. As many other teleost fishes, zebrafish (Danio rerio) is a good candidate to investigate the behavioural effects of approach–avoidance conflicts because of its genetic and neurophysiological proximity with “higher” vertebrates. The present study aims to determine how Zebrafish reacted to threats of different magnitude (low vs. high) following the delivery of food. [less ▲]

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See detailResorcinol-formaldehyde carbon xerogels as lithium-ion battery anode materials: influence of porosity on capacity and cycling behaviour
Piedboeuf, Marie-Laure ULiege; Léonard, Alexandre ULiege; Khomenko, Volodymyr et al

Poster (2012, July 05)

Carbon xerogels are promising candidates in the development of new high performance C-based anode materials for Li-ion batteries. Indeed, their specific capacities widely exceed that of conventional ... [more ▼]

Carbon xerogels are promising candidates in the development of new high performance C-based anode materials for Li-ion batteries. Indeed, their specific capacities widely exceed that of conventional graphitic structures, and they can be intercalated/deintercalated in a low-cost electrolyte based on propylene carbonate (PC), which has an excellent conductivity at low temperatures. In addition, such carbonaceous materials show very small changes of volume during the charge/discharge, providing a long cycle life of such an anode. Nevertheless, hard carbons also exhibit quite high irreversible capacity losses due to their intrinsic high microporosity and, compared to graphite, a poor rate performance related to slow diffusion of Li in the internal structure[1]. To reduce these disadvantages, the structural and textural characteristics need to be carefully controlled. Porous carbon xerogels can easily be prepared from resorcinol-formaldehyde aqueous mixtures, which are polymerized, dried and pyrolysed. The porosity of these xerogels is mainly governed by the pH of the precursor solution as well as by the drying procedure. Globally, these materials are composed of microporous nodules delimiting meso- or macroporous voids, the size of which is adjusted via the synthesis pH. Too a high microporosity can induce considerable irreversible capacity losses and too small mesopores may hinder the proper chemical diffusion of lithium ions within a bulk electrode material. The latter is often a rate-limiting step and optimized transport pathways could be provided by creating large mesopores or even macropores within the microporous carbon [3]. Here we report on the preliminary electrochemical characterization of porous carbon xerogels prepared by vacuum drying procedure. By adjusting the pH of the precursor solution, the materials obtained develop low to high values of specific surface areas and exhibit homogeneous pore sizes that range from several microns to several nanometers. The electrochemical performance of these materials as electrode compounds was tested by galvanostatic charge-discharge of 16-mm disc electrodes assembled in CR2016 coin cells or of 13-mm disc electrodes in home-made Swagelok-type cells. The first results show that all the samples show quite a high irreversible capacity during the first cycle; this irreversible capacity is proportional to the specific surface area. Its value nevertheless remains quite low for the low-surface area macroporous sample. The latter also shows the best reversible capacity after the second cycle, with values approaching that of commonly used graphite. For example, when cycled at a rate of C/20 for 10 cycles, this sample showed a capacity of 320 mAh/g; the value was kept at 200 mAh/g when increasing the rate up to C/5. The long-term cycling performance was investigated by cycling the anodes at C/20 and C/5. Again, the macroporous sample behaves best, with superior capacity retention and invariable discharge capacity of ~175 mAh/g after more than 100 cycles. The electrochemical properties of carbon xerogels was evaluated in the conditions which are used typically for graphite (cycles in the potentials range from 0.003 to 1.5 V vs. Li+/Li). A higher reversible capacity of 400 mAh/g could be obtained for the macroporous sample using a discharge with plating of Li as described in [4], but this method could not be accepted in the case of Lithium-ion batteries. These first results show that carbon xerogels are very promising candidates as anode materials for Li batteries, providing the textural characteristics are carefully controlled. The ongoing work is dealing with the establishment of possible relationships between textural features and electrochemical performance in order to shed light on the requirements that will dictate the best synthesis procedures. References: [1] T. Tran, B. Yebka, X. Song, G. Nazri, K. Kinoshita and D. Curtis, J. Power Sources, 85, 269, 2000. [2] N. Job, A. Théry, R. Pirard, J. Marien, L. Kocon, J.-N. Rouzaud, F. Béguin and J.-P. Pirard, Carbon 43, 2481, 2005. [3] F. Cheng, Z. Tao, J. Liang, and J. Chen, Chem. Mater., 20, 667, 2008. [4] W. Xing, J. S. Xue, J.R. Dahn, J. Electrochem. Soc, 143, 3046, 1996. [less ▲]

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See detailFunctional study of Arabidopsis thaliana ASF/SF2-like pre-mRNA SR splicing factors
Stankovic, Nancy ULiege; Tillemans, Vinciane; Leponce, Isabelle et al

Poster (2012, July 04)

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See detailEnteroendocrine cells ontogenesis in zebrafish
Stern, David ULiege; Voz, Marianne ULiege

Poster (2012, July 04)

Endocrine cells of the digestive tract include pancreatic cells clustered in the islets of Langerhans and the enteroendocrine cells, scattered throughout the digestive epithelium. Notch pathway plays a ... [more ▼]

Endocrine cells of the digestive tract include pancreatic cells clustered in the islets of Langerhans and the enteroendocrine cells, scattered throughout the digestive epithelium. Notch pathway plays a crucial role in endocrine cell fate determination and mediates cell fate decisions. The goal of this project is to decipher the molecular cascade triggered by Notch signaling that controls the endocrine cell differentiation in the digestive system. The targets of Notch signaling are usually members of the bHLH family and more precisely of the Achaete scute-like (Ascl) family or of the atonal related proteins (ARP) family. In this study, we searched for all ARP and Ascl factors expressed in the endocrine lineage of the pancreas and in the gastrointestinal tract in zebrafish.   [less ▲]

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See detailConverting the legend of the Soil Map of Belgium into the World Reference Base for Soil Resources: Lessons from correlating national soil survey data to an international soil classification system
Bouhon, Antoine; Dondeyne, Stefaan; Legrain, Xavier ULiege et al

Poster (2012, July 03)

Soils in Belgium were mapped between 1947 and 1991 and published at a 1:20000 scale. These maps are used in land consolidation projects and for assessing soils’ vulnerability to erosion and pollution ... [more ▼]

Soils in Belgium were mapped between 1947 and 1991 and published at a 1:20000 scale. These maps are used in land consolidation projects and for assessing soils’ vulnerability to erosion and pollution. Integration of land-use and environmental policies within the European Union however requires a harmonization of different national soil classification systems. With the World Reference Base for Soil Resources (WRB) as common classification system within the Union, the authorities of Flanders and Wallonia commissioned a study to elaborate a methodology for converting the Belgian soil legend into WRB. The Belgian legend is based on field properties such as texture, drainage status and profile development. The WRB classification is based on diagnostic features defined by morphological, physical and chemical properties. A key and software programme have been developed to convert the Belgian units into WRB units. However, as many Belgian units could not unequivocally be translated into WRB units, additional guidelines had to be derived based on soil survey data classified according to WRB. The data show that principles of the legend shifted over time or were interpreted differently to take regional specificities into account. To overcome resulting ambiguities it is proposed to establish a database of reference soil profiles. Whereas, overall WRB is satisfactory for classifying soils at national level, the experience also shows that some WRB concepts may benefit from revisions to facilitate its correlation with national soil survey data. [less ▲]

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See detailModeling of the Diffusion of VOCs Emitted by Barley Roots
Hirtt, Laura ULiege; Destain, Marie-France ULiege; Lognay, Georges ULiege et al

Poster (2012, July 02)

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See detailHydrological instrumentation of a pilot catchment in view to improve the soil loss modeling: focus on the spatial distribution of erosion and deposition (loamy region, Belgium)
Pineux, Nathalie ULiege; Degré, Aurore ULiege

Poster (2012, July 02)

Nowadays, lots of catchments are affected by inundations or mudflows which are the consequences of excessive runoff and accelerated erosion. That generates also a significant loss of arable land. Yet, the ... [more ▼]

Nowadays, lots of catchments are affected by inundations or mudflows which are the consequences of excessive runoff and accelerated erosion. That generates also a significant loss of arable land. Yet, the soil resource is not an unlimited commodity. Moreover, sediments’ transfer to watercourses alters their physical and chemical quality. The watershed management should aim at both limiting erosion and enhancing deposition in appropriate zones. Therefore, the global objective of this work is to acquire hydropedological data in order to better quantify the erosion and deposition phenomenon in Belgium. The poster presents the field monitoring put in place in our experimental watershed. Indeed, observed quantitative data are essential but still limited. Particularly, we lack observations spatially distributed on the watershed. The watershed is a 124 ha agricultural zone in the loamy region. Its slopes range from 0% to 9%. Instrumentation includes a weather station with disdrometer, discharge measurement at the outlet coupled with water sampling. Fields observations are done to determine the texture redistribution and compared with a previous soil survey realised in 1958. Moreover, regular flights above the area will allow us to obtain a very accurate DEM using Lidar technology (5cm pixel) and observing the relief evolution. The CAESAR model will be tested on this watershed. It aims at representing both erosion and sedimentation and estimates the net erosive flows. This model is based on the Einstein-Brown equations and needs an initial digital elevation model, hourly rainfalls, soil texture, etc. It produces a digital elevation models’ evolution through time. [less ▲]

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See detailImpact of soil management on earthworm diversity according to differential plowing and plant residue incorporation
Lemtiri, Aboulkacem ULiege; Alabi, Taofic; Zirbes, Lara ULiege et al

Poster (2012, July 02)

Earthworms are largely distributed in terrestrial ecosystems and their abundance and diversity in soils are significantly affected by biotic (macro- and micro-organisms) and abiotic factors: soil ... [more ▼]

Earthworms are largely distributed in terrestrial ecosystems and their abundance and diversity in soils are significantly affected by biotic (macro- and micro-organisms) and abiotic factors: soil properties (pH, texture, structure…); agricultural management system and climate change. Here, tillage effect of earthworm population combined with crops residual management was investigated and correlated with soils properties. From wheat experimental field plots, the diversity of earthworm according to the field crop management was assessed. Application of particular crop production practices such as the integration of different levels of crop residues, diverse parts of wheat straws, at the field level regulate earthworm diversity and population abundance. Indeed, tillage reduced earthworm population with a 35% rate also corresponding to changes in soil properties. Agricultural practices had to be adapted to include consideration on macro-invertebrate abundance and diversity to maintain efficient soil fertility and allow sustainable crop production [less ▲]

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See detailAntarctic Microbial BIOdiversity : the importance of geographical versus ecological factors
Obbels, Dagmar; De Carvalho Maalouf, Pedro ULiege; De Wever, Aaike et al

Poster (2012, July)

Antarctica is a prime region to test whether microbes have a biogeography and to study their metacommunity dynamics, because (i) it is isolated from the other continents, (ii) its extreme environmental ... [more ▼]

Antarctica is a prime region to test whether microbes have a biogeography and to study their metacommunity dynamics, because (i) it is isolated from the other continents, (ii) its extreme environmental conditions allow microorganisms to dominate its ecosystems, and (iii) lacustrine and terrestrial habitats occur isolated in a matrix of ice and ocean. We compiled a large set of samples from benthic microbial mats from Antarctic lakes in different ice-free regions and used a polyphasic approach to study their microbial biodiversity by combining morphological characterization of diatoms with molecular techniques such as Denaturing Gradient Gel Electrophoresis (green algae and cyanobacteria), 454 pyrosequencing and cultivation (prokaryotes). [less ▲]

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See detailRadial arm maze as a new paradigm to study collective behaviours in fish
Delcourt, Johann ULiege; Garnier, Simon; Miller, Noam Y. et al

Poster (2012, July)

Collective decision-making is based on both environmental information perceived by individuals and social interactions with other group members. Determining and analyzing separately both interactions is a ... [more ▼]

Collective decision-making is based on both environmental information perceived by individuals and social interactions with other group members. Determining and analyzing separately both interactions is a real challenge. If the environmental influences on group behaviours can be determined, new possibilities to collect information about processes inside the group become possible. To improve our knowledge of these processes, an experiment where collective decision-making can be measured easily and without any ambiguity is needed. For this perspective, a new paradigm in the study of collective behaviour is introduced here. The radial arm maze is a classical method used to study individual cognitive abilities. Its advantages are firstly to allow control of environmental information; secondly, to realize multi-way tests, and thirdly, to give the opportunity to collect categorical responses like presence/absence. We apply this paradigm for the first time to a whole animal group. We have also developed an image analysis system able to automatically count the number of individuals in every defined zone. Due to this counting, the degree of cohesion, the group stability, the activity and zone preferences can be described as function of factors such as the group size, the defined zones, or the experimental time. The degree of cohesion can be measured by a new index taking into account the number of sub-groups and the size of each ones. Group activity can be measured by the movement of the majority group between arms. This activity allows determining exploratory processes but also whether zone preferences or homing phenomena appear in the absence of any stimulus. To illustrate, our first results from the exploratory behaviour study of shoals of golden shiners (Notemigonus crysoleucas) are introduced. Using this new paradigm, it is now possible to quantify rapidly in a standardised way the collective responses of fish shoals according to the absence or presence of environmental stimuli, and to create experiments where environmental information is controlled. [less ▲]

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See detailGenomic selection and scan for major genes for a new lamb survival trait for the New Zealand sheep industry
Auvray, Benoit; Vanderick, Sylvie ULiege; Newman, Sheryl-Anne et al

Poster (2012, July)

Lambing percentage is one of the most significant factors affecting profitability on New Zealand sheep farms. Since the early 1990s, lambing percentage has increased at about 1% per year from a relatively ... [more ▼]

Lambing percentage is one of the most significant factors affecting profitability on New Zealand sheep farms. Since the early 1990s, lambing percentage has increased at about 1% per year from a relatively stable level of approximately 100%, and top performing sheep farms are now consistently achieving 150% or more. As lambing percentage increases, the proportion of ewes bearing twins and triplets increases accordingly. Lamb mortality rate in these multiples is higher than in singles, with triplets being particularly susceptible. Consequently, lamb survival has become increasingly important to the New Zealand sheep industry. Sheep Improvement Ltd. (SIL, New Zealand’s national sheep genetic evaluation system owned by Beef + Lamb NZ) records lamb survival to weaning but genetic improvement has been limited due to the low heritability of the trait and the current method of recording. To address those issues, we have developed an improved survival to weaning trait for industry implementation, which is more accurate and more heritable than the current SIL trait. This poster will present results of applying genome-enabled prediction procedures to the new trait to obtain molecular breeding values. It will also describe results from a genome wide association study using the new trait. [less ▲]

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See detailModeling argon dynamics in first-year sea ice
Moreau, S.; Vancoppenolle, M.; Tison, J.-L. et al

Poster (2012, July)

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See detailHepatitis E virus infection of wild boar (Sus scrofa) in southern Belgium.
Thiry, Damien ULiege; Mauroy, Axel ULiege; Brochier, Bernard et al

Poster (2012, July)

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See detailA 40-year accumulation dataset for Adelie Land, Antarctica and its application for model validation
Agosta, Cécile ULiege; Favier, Vincent; Genthon, Christophe et al

Poster (2012, July)

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See detailAtomic decay data for modeling K lines of iron peak and light odd-Z elements
Palmeri, P.; Quinet, Pascal ULiege; Mendoza, C. et al

Poster (2012, July)

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See detailExamining the development of morphological representations in developing readers: a self-teaching study
Quemart, Pauline ULiege; Casalis, Séverine

Poster (2012, July)

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See detailTransposability and evaluation of pedotranfer functions for predicting properties of water retention on soils of low chelif. Algeria
Touil, Sami; Saidi, Djamel; Degré, Aurore ULiege

Poster (2012, July)

An important question remains about PTF’s transposability to others agropedoclimatic contexts. Models developed and validated in a particular bioclimatic context, were relatively little tested in other ... [more ▼]

An important question remains about PTF’s transposability to others agropedoclimatic contexts. Models developed and validated in a particular bioclimatic context, were relatively little tested in other contexts. The evaluation of PTF to estimate water retention at field capacity pF 2.5 (-330 hPa) and at wilting point pF 4.2 (-15000 hPa) of some soils of Lower Cheliff is presented. [less ▲]

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See detailEffects of cold temperatures on the early stages of maize inbreds
Riva-Roveda, Laetitia ULiege; Escale, Brigitte; Giauffret, Catherine et al

Poster (2012, July)

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See detailBiophysical Approaches Modelling the Interaction of the Antimicrobial Mycosubtilin with Sterol‐Containing Membranes of Sensitive Cells
Nasir, Mehmet Nail ULiege; Loison, Claire; Benichou, Emmanuel et al

Poster (2012, July)

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See detailSimulations of the Antifungal Lipopeptide Mycosubtilin in Langmuir Monolayers of Sterols
Loison, Claire; Nasir, Mehmet Nail ULiege; Besson, Françoise

Poster (2012, July)

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See detailDevelopment of semiochemical slow-release formulations as biological control devices
Heuskin, Stéphanie ULiege; Lorge, Stéphanie ULiege; Leroy, Pascal ULiege et al

Poster (2012, July)

Semiochemicals have been widely considered within various integrated pest management (IPM) strategies. In the present work, two sesquiterpenoids, E-β-farnesene and E-β-caryophyllene, were formulated for ... [more ▼]

Semiochemicals have been widely considered within various integrated pest management (IPM) strategies. In the present work, two sesquiterpenoids, E-β-farnesene and E-β-caryophyllene, were formulated for their related properties as aphid enemy attractants. E-β-farnesene, the alarm pheromone of many aphid species, was also identified as a kairomone of aphid predators (Episyrphus balteatus De Geer (Diptera: Syrphidae)) and parasitoids (Aphidius ervi Haliday (Hymenoptera: Braconidae)). E-β-caryophyllene was identified as a potential component of the aggregation pheromone of the Asian ladybird, Harmonia axyridis Pallas, another aphid predator. The two products were purified from essential oils of Matricaria chamomilla L. (Asteraceae) and Nepeta cataria L. (Lamiaceae) for E-β-farnesene and E-β-caryophyllene, respectively. Natural and biodegradable slow-release formulations were then investigated in order to deliver these molecules on crop fields for a long period of time as biological control devices. Due to their sensitivity to oxidation, both sesquiterpenes needed to be protected from degradation. For this purpose, alginate – hydrophilic matrix with low oxygen permeability – was used as polymer for the formulations: the main objective was to deliver semiochemical substances in the air in a controlled way. Consequently, a careful selection of alginates was realised. Formulated beads showed different structural and encapsulation properties depending on various formulation factors. Alginate formulations were characterized by texturometry and by confocal microscopy in order to observe the distribution of semiochemicals in alginate network. The last step of alginate bead characterisation consisted in studying release rate of semiochemicals in laboratory-controlled conditions by optimised trapping and validated Fast-GC procedures. Finally, the efficiency of formulations as aphid predator (Syrphidae) and parasitoid (A. ervi) attractants was demonstrated by field trapping and olfactometry experiments. [less ▲]

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See detailIncreased cell proliferation in Seriatopora hystrix following heat-induced bleaching
Fransolet, David ULiege; Ugille, Aurélie; Leblud, Julien et al

Poster (2012, July)

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See detailFlexible aggregative behavior of Harmonia axyridis according to the freshness of area marking in overwintering sites
Durieux, Delphine ULiege; Fischer, Christophe ULiege; Fassotte, Bérénice ULiege et al

Poster (2012, July)

The multicolored Asian ladybeetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), aggregates inside dwellings and buildings during winter to survive cold. This adaptive behavior causes annoyances ... [more ▼]

The multicolored Asian ladybeetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), aggregates inside dwellings and buildings during winter to survive cold. This adaptive behavior causes annoyances to the occupants because of their large number and the induction of allergic reactions. Although this species has aroused a great interest these last years, the factors involved in the selection of its overwintering sites remain misunderstood. The work presented herein was oriented to the study of the non-volatile chemical compounds involved in this aggregation behavior. Chemical analyses revealed the occurrence, in aggregation sites, of an area marking made up of saturated and unsaturated hydrocarbons. Behavioral investigations demonstrated that H. axyridis preferentially aggregates in sites previously marked by congeners, indicating the retention potential of this blend on overwintering individuals. In the second instance, the same analyses were performed on an area marking aged of one year. The chemical investigations showed that only saturated hydrocarbons can still be detected after that period of time but the remaining blend does not induce aggregation anymore. This difference of response according to the freshness of the area marking suggests that this species would not be prisoner of the marking previously deposited on the substrate if the surrounding has changed and the site is not suitable anymore. [less ▲]

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See detailDRIVING FACTORS OF SOIL FERTILITY IN MOUNTAIN TERRACED PADDY FIELDS OF YUANYANG (CHINA)
Colinet, Gilles ULiege; Wu, Bozhi; Li, Yongmei et al

Poster (2012, July)

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See detailComparison of Staphylococcus aureus strains isolated from bovine mastitis
Bardiau, Marjorie ULiege; Duprez, Jean-Noel; Mainil, Jacques et al

Poster (2012, July)

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See detailNon-invasive monitoring of mixed cropping systems. A case-study in Ratchaburi province, Thailand
Garré, Sarah ULiege; Coteur, Ine; Diels, Jan et al

Poster (2012, July)

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See detailParticle acceleration in colliding-wind massive binaries: a relevant science case for ASTRO-H
De Becker, Michaël ULiege; Khangulyan, Dmitry; Bosch-Ramon, Valenti

Poster (2012, July)

The strong stellar winds of massive stars in binary systems interact through shocks responsible for several phenomena, including significant particle acceleration up to relativistic energies. The ... [more ▼]

The strong stellar winds of massive stars in binary systems interact through shocks responsible for several phenomena, including significant particle acceleration up to relativistic energies. The existence of this relativistic particle population is mainly revealed through detection of bright synchrotron radio emission in the case of a few tens of systems. More recently, Suzaku observations revealed the existence of non-thermal X-rays in the case of two colliding-wind binaries (CWBs), confirming the prediction that inverse Compton scattering should be at work in these objects. In this context, the ASTRO-H mission constitutes the ideal tool to investigate non-thermal phenomena in hard X-rays (above 10 keV), where the well-known thermal emission from the shocked winds should not be significantly present. This poster gives an overview of this science case, and provides clues for the expected input of ASTRO-H in the study of these objects. [less ▲]

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See detailNon-thermal Hard X-ray Emission from Colliding Wind Binary Systems
Khangulyan, Dmitry; Bosch-Ramon, Valenti; De Becker, Michaël ULiege

Poster (2012, July)

Colliding-wind massive star binaries are known to produce strong shocks in their wind-wind interaction regions. The interaction region is the scenario of several physical processes, including strong ... [more ▼]

Colliding-wind massive star binaries are known to produce strong shocks in their wind-wind interaction regions. The interaction region is the scenario of several physical processes, including strong thermal X-ray emission and in several cases particle acceleration up to relativistic energies. The latter process is still poorly understood in these environments, and deserves a particular attention in stellar astrophysics. The investigation of particle acceleration is a critical aspect of high-energy astrophysics, especially at energies above 10 keV where non-thermal emission processes dominate. We discuss the possibility to detect the hard X-ray emission from WR147, a well-known particle accelerator, using Astro-H Hard X-ray Telescope (HXT) and Soft Gamma-Ray Detector (SGD), and to probe the particle acceleration process at work in its colliding-wind region. [less ▲]

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See detailClimate change simulation in continental Antarctica using Open-Top Chambers
Mano, Marie-José ULiege; Namsaraev, Zorigto; Gorodetskaya, Irina et al

Poster (2012, July)

In continental Antarctica, the environnmental conditions are extreme and only microbial organisms can withstand them. Currently, the majority of OTCs experiments are being held in Maritime Antarctica but ... [more ▼]

In continental Antarctica, the environnmental conditions are extreme and only microbial organisms can withstand them. Currently, the majority of OTCs experiments are being held in Maritime Antarctica but it would be interesting to have such data for the continental part of Eastern Antarctica. To monitor the response of the microbial communities to local simulations of climate change, 8 Open-Top Chambers (OTC) were installed close to the Princess Elisabeth station, in the Sor Rondane Mountains in January 2010. They are located on the Utsteinen ridge, the Tanngarden granite outcrop, the Teltet nunatak and the fourth nunatak of the Pingvinane range. In each location, two OTCs and a control area were established. Temperature and humidity loggers were installed inside the OTCs and outside, in the control areas, to estimate the environmental changes induced by the OTCs. [less ▲]

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See detailDiversity of the cyanobacterial communities from the Sør Rondane Mountains (Eastern Antarctica)
Mano, Marie-José ULiege; Namsaraev, Zorigto; Fernandez, Rafael et al

Poster (2012, July)

The new Belgian “Princess Elisabeth” research station was built in 2009 and is located 200 km inland in the Western part of the Sør Rondane Mountains (Eastern Antarctica). The BELSPO projects ANTAR-IMPACT ... [more ▼]

The new Belgian “Princess Elisabeth” research station was built in 2009 and is located 200 km inland in the Western part of the Sør Rondane Mountains (Eastern Antarctica). The BELSPO projects ANTAR-IMPACT and BELDIVA aimed to evaluate the diversity and the distribution patterns of the microorganisms from different types of habitats in a radius of 50 km around the Belgian station. These data will serve to follow future anthropogenic and climatic impacts on these communities. Here, we focus on the diversity of cyanobacteria. [less ▲]

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See detailCalculation of Thermoelectric Properties from First-Principles
Xu, Bin ULiege; Verstraete, Matthieu ULiege

Poster (2012, July)

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See detailNon-radial, non-adiabatic solar-like oscillations in RGB and HB stars
Grosjean, Mathieu ULiege; Dupret, Marc-Antoine ULiege; Belkacem, Kevin et al

Poster (2012, July)

Corot and Kepler observations of red giants reveal rich spectra of non-radial solar-like oscillations allowing to probe their internal structure. We compare the theoretical spectrum (amplitudes and life ... [more ▼]

Corot and Kepler observations of red giants reveal rich spectra of non-radial solar-like oscillations allowing to probe their internal structure. We compare the theoretical spectrum (amplitudes and life- times) of two red giants in the same region of the HR diagram but in different evolutionary phases. The lifetimes are obtained by computing theoretical non-adiabatic non-radial solar-like oscillations for mixed modes in the two models. Thanks to this, we have been able to compute the oscillation amplitudes through a stochastic excitation model. We present here our first results on the inertia, damping rates and amplitudes of the oscillations in the two stars and discuss the trapping, the visibilities and the am- plitudes of the different modes. The differences in the spectra of the two stars are also investigated. As already known, the period spacings in the two models are very different. Moreover, we find significant differences in amplitudes and lifetimes between the two models. [less ▲]

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See detailLitterfall quantity and quality and nutrient status in a young stand under Norway spruce and broadleaved species
Bazgir, Masoud; Carnol, Monique ULiege

Poster (2012, July)

In Europe, because of previous intense forest use in the past centuries, the plantation of coniferous stands, especially Norway spruce with high production potential has been a common strategy. However ... [more ▼]

In Europe, because of previous intense forest use in the past centuries, the plantation of coniferous stands, especially Norway spruce with high production potential has been a common strategy. However, the characteristics of Norway spruce such a s susceptibility to windfall, forest dieback and soil acidification have caused negative ecological impacts at many sites. Conversion of conifers into deciduous or mixed stands has been suggested in order to improve ecological conditions and biodiversity o f forest ecosystems. In this study, six broadleaved species were planted at the same site, thus being subjected to similar condition in terms of soil type, land use history and climate. The aim of this research was to study of foliar nutrient status and nu trient return to the forest floor through litterfall 11 years after conversion of Norway spruce (Picea abie s (L.) KARST.) into a mixed stand with common alder (Alnus glutinosa (L.) GAERTN.), european beech (Fagus sylvatica L.), pedunculate oak (Quercus rob ur L.), silver birch (Betula pendula ROTH.), goat willow (Salix caprea L.) and rowan (Sorbus aucuparia L.). Our results revealed that tree species had a different foliar nutrient status and an effect on nutrient input fluxes through litterfall. For example , litter nutrient contents (N, Ca, Mg, K) were highest under alder, rowan and willow. Total litterfall and nutrient fluxes from leaf litter were highest under rowan. Our results demonstrate that, on poor sites, plantation of pioneer species, especially row an, may improve the nutrient status of the forest floor through higher input in litterfall. [less ▲]

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See detailEffects of tree species diversity on earthworm communities in European forests
de Wandeler, Hans; Ottoy, Sam; Hermy, Martin et al

Poster (2012, July)

The belowground food web holds a big part of the associated biodiversity in forest ecosystems and plays a major role in essential ecosystem processes, e.g. litter decomposition and nutrient turnover. So ... [more ▼]

The belowground food web holds a big part of the associated biodiversity in forest ecosystems and plays a major role in essential ecosystem processes, e.g. litter decomposition and nutrient turnover. So far, important interactions between diversity and composition of above-and belowground food webs have been observed. However the effects of tree species diversity on the belowground food web are so far not conclusive. This study aims at elucidating the effect of tree species mixtures, species diversity and trait diversity on the composition of the earthworm communities in European forests. Experimental platforms of planted tree species diversity assemblages in Finland (Satakunta) and Germany (Biotree) are therefore intensively sampled for earthworms using the combined mustard extraction/hand sorting method. First results are reported and discussed and diversity effects are evaluated with overyielding tests. [less ▲]

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See detailPhenolic compounds in apple juices - Method of quantification by UHPLC-UV and UHPLC-MS/MS
Verdu, Cindy ULiege; Gatto, Julia; Freuze, Ingrid et al

Poster (2012, July)

An UPHLC-UV/MS/MS method was developed for separation and quantification of major polyphenolic compounds present in apple juice. The main classes of polyphenols studied here are monomeric or polymeric ... [more ▼]

An UPHLC-UV/MS/MS method was developed for separation and quantification of major polyphenolic compounds present in apple juice. The main classes of polyphenols studied here are monomeric or polymeric flavan-3-ols, phenolic acids, flavonols and dihydrochalcones. compounds were quantified simultaneously with the two detection systems, i.e. with UV-PDA detection and ESI-triple quadrupole mass detection used in the selected reaction monitoring (SRM) mode. Each method (UHPLC-UV and UHPLC-SRM) was assessed with 2 different apple juice samples and linearity, limit of detection and quantification (LOD and LOQ), accuracy and precision tests were realized on the 15 major compounds. Despite the validation of the UHPLC-UV and UHPLC-SRM methods, significantly different results were obtained by comparing UHPLC-UV and UHPLC-SRM quantifications. Three groups of polyphenols were observed, the first one with equivalent results between the two quantification methods (in most cases), and the two others with overestimated or underestimated results when comparing UHPLC-UV and UHPLC-SRM quantifications. [less ▲]

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See detailContribution of new radiocarbon dates to track the impact of past anthropogenic disturbances on current vegetation in Central Africa
Morin-Rivat, Julie ULiege; Gillet, Jean-François ULiege; Bourland, Nils ULiege et al

Poster (2012, July)

Introduction: Understanding current Central Africa vegetation patterns faces the scarcity of data about their past evolution. However, a growing hypothesis suggests that past human activities could have ... [more ▼]

Introduction: Understanding current Central Africa vegetation patterns faces the scarcity of data about their past evolution. However, a growing hypothesis suggests that past human activities could have had a substantial influence on vegetation (Van Gemerden et al. 2003, Brncic et al. 2009). Indeed, by creating large openings (fig. 1), they might have triggered the expansion of light-demanding species currently suffering from a lack of regeneration. This lack of saplings could have been caused by the change in land use since colonization with the sedentarization of shifting cultivators. Aim of the study: To investigate the potential relationship between past anthropogenic disturbances and present vegetation. Material & methods: Fieldworks combining anthracological and ecological approaches have been undertaken in Northern Congo and South-Eastern Cameroon (fig. 2). We excavated thirty 150-200 cm deep pits under different forest covers and vegetation types to identify evidences of past human presence (i.e potsherds, fig. 3, slags from metallurgy, anthropogenic pieces of charcoals and anthropophilous charred seeds). Charcoals and seeds (oil palm Elaeis guineensis, fig. 4, Canarium schweinfurthii) in combination with artifacts have been dated. Results: A set of 38 new radiocarbon dates ranging from 15,200 cal BP to present time have been obtained. They confirm the existence of important past fire events in a region where natural ones seldom occur. Together with artifacts, our findings support the few already available dates documenting evidences of past human activities in Central African rainforests. On the 36 most recent dates (fig. 5), the majority belongs to the 2,300-1,400 cal BP period (61%). The whole semi-deciduous forest zone is concerned by this period of intense disturbances with a high rate of fragmentation. It follows the last great arid phase ca. 2,500 cal BP and might be linked to the iron workers expansion. Another pool of dates between 650 and 250 cal BP (33%) associated with potsherds might be correlated to a dry phase contemporary to the Little Ice Age in Europe. That last group of dates points out the potential positive impact of anthropogenic disturbances connected to a dry climatic event on light-demanding species populations. Indeed, this is consistent with the fact that most of current light-demanding trees have a higher number of stems around 100 cm dbh. Conclusion: Our multidisciplinary approach allowed new insights into the link between human history and vegetation dynamics in Central Africa. Further investigations should be conducted to go deeper into the understanding of the evolution of Central African rainforests and to improve the management of currently logged light-demanding species resulting from the LIA period. Thirty new dates from sixteen soil profiles are forthcoming. References: Brncic T., Willis M., K. J., Harris D. J., Telfer M. W. & Bailey M. W. 2009. Fire and climate change impacts on lowland forest composition in northern Congo during the last 2580 years from palaeoecological analyses of a seasonally flooded swamp. The Holocene, 19, 79-89. Reimer P. J., Baillie M. G. L., Bard E., Bayliss A., Beck J. W., Blackwell P. G., Bronk Ramsey C., Buck C. E., Burr G. S., Edwards R. L., Friedrich M., Grootes P. M., Guilderson T. P., Hajdas I., Heaton T. J., Hogg A. G., Hughen K. A., Kaiser K. F., Kromer B., McCormac F. G., Manning S. W., Reimer R. W., Richards D. A., Southon J.R., Talamo S., Turney C. S. M., van der Plicht J., & Weyhenmeyer, C. E. 2009. IntCal09 and Marine09 radiocarbon age calibration curves, 0-50,000 years cal BP. Radiocarbon, 51(4), 1111-1150. Van Gemerden B. S., Olff H., Parren M. P. E, Bongers F. 2003. The pristine rain forest? Remnants of historical human impacts on current tree species composition and diversity. Journal of Biogeography, 30, 1381-1390. [less ▲]

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See detailWavelet Spectrum Estimation under Chronology Uncertainties Application to Dust Flux Data from Belgian Fagnes
Lenoir, Guillaume; Allan, Mohammed ULiege; Crucifix, Michel et al

Poster (2012, July)

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See detailNuclear genetic structure of Myodes glareolus in Fennoscandia with a particular emphasis on the contact zones - Preliminary results
Pisano, Julie ULiege; Henttonen, Heikki; Galan, Maxime et al

Poster (2012, July)

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See detailRadiative parameters for lowly charged tungsten ions of interest in fusion plasma research
Quinet, Pascal ULiege; Biémont, Emile ULiege; Palmeri, P. et al

Poster (2012, July)

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See detailMid and late Holocene dust deposition in Western Europe: The Misten peat bog (Hautes Fagnes - Belgium)
Allan, Mohammed ULiege; Fagel, Nathalie ULiege; Le Roux, Gael et al

Poster (2012, June 28)

The Misten peat bog representing 7.5 m of peat accumulation in the Hautes-Fagnes Plateau, Belgium, provides a record of Rare Earth Elements (REE) deposition since more than 7000 years. The analyses of REE ... [more ▼]

The Misten peat bog representing 7.5 m of peat accumulation in the Hautes-Fagnes Plateau, Belgium, provides a record of Rare Earth Elements (REE) deposition since more than 7000 years. The analyses of REE and lithogenic element concentrations, as well as the Nd isotopes, were performed by HR-ICP-MS and MC-ICP-MS, respectively in peat layers previously dated by 210Pb and 14C. REE concentration variations in peat samples are correlated with Ti, Zr and Sc that are lithogenic conservative elements, suggesting that REE are immobile in the studied peat bogs [1] and can be used as tracers of dust deposition. Peat humification, C/N ratio, ash content and bulk density were used to evaluate hydroclimatic conditions. The Nd values show large variability, between +1 to –22, identifying three major sources of dusts falling into the peat: local soils, distal volcanic and desert particles. More recently, industrial emissions provide a fourth source of dusts [2], which is also clearly recorded in the last 200 years of the Misten peat profile. [less ▲]

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See detailSynthesis of lutetium-carrying oligonucleotides for targeted cancer therapy and imaging
Gijs, Marlies ULiege; Aerts, An; Impens, Nathalie et al

Poster (2012, June 27)

In this study, different techniques were evaluated to test the binding properties of an anti-HER3 aptamer to its target on cells or as pure protein. Furthermore, the aptamer was successfully coupled to ... [more ▼]

In this study, different techniques were evaluated to test the binding properties of an anti-HER3 aptamer to its target on cells or as pure protein. Furthermore, the aptamer was successfully coupled to DOTA-NHS ester and labelled with natural occurring lutetium. However, for a better yield, the coupling and labelling reactions need further optimization. [less ▲]

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See detailCoupling long term database with SWAT and STICS models for testing modles and simulating nitrogen management scenarios
Deneufbourg, Mathieu ULiege; Pugeaux, Nicolas; Huguet, Jean et al

Poster (2012, June 26)

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See detailThe meeting preparation task: Validation of a new measure of everyday life functioning with persons diagnosed with schizophrenia.
Laloyaux, Julien ULiege; Levaux, Marie-Noëlle ULiege; Mourad, Haitham et al

Poster (2012, June 25)

Objective: The objective was to create a new measure of everyday life functioning by developing a computerized real-life activity task where participants are asked to prepare a room for a meeting ... [more ▼]

Objective: The objective was to create a new measure of everyday life functioning by developing a computerized real-life activity task where participants are asked to prepare a room for a meeting. Background: Difficulties in everyday life activities are core features of persons diagnosed with schizophrenia. However, everyday life functioning is usually evaluated with questionnaires, performance-based or observation assessments, all of which contain a number of limits. Method: Twenty-one individuals diagnosed with schizophrenia and 21 matched healthy controls completed the computerized meeting preparation task. Patients were also evaluated with an extensive cognitive battery and several measures of symptomatology and everyday life functioning. Results: Results showed that performance on the computerized task significantly differentiated patients and controls for a certain number of variables. These variables, summarized in a global score, were significantly correlated with both cognitive and everyday life functioning. Moreover, this composite score significantly predicted 39% of everyday life functioning, whereas a cognitive composite score only predicted 22%. In addition, when the meeting task composite score was combined with symptomatology and the cognitive composite score, these measures significantly predicted up to 73% of everyday life functioning. Conclusion: The findings suggest that the meeting preparation task provides a good indication of the level of everyday life functioning in patients with schizophrenia, and may be viewed as a valuable instrument in both an evaluation and remediation context. [less ▲]

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See detailDoes fluid intelligence protect from age-related decline in cognitive control ?
Manard, Marine ULiege; Carabin, Delphine; Collette, Fabienne ULiege

Poster (2012, June 25)

Age-related difficulties have been reported on proactive control whereas reactive control seems to remain intact. This study investigated the potential influence of fluid intelligence capacity on the age ... [more ▼]

Age-related difficulties have been reported on proactive control whereas reactive control seems to remain intact. This study investigated the potential influence of fluid intelligence capacity on the age-related decline in proactive control. We used a working memory recognition paradigm involving proactive or reactive cognitive control by manipulating the interference level across items. 80 young adults (18-29 years old) and 80 healthy older adults (60-89 years old) were included. The main results revealed significant effects of age and fluid intelligence capacity on sensitivity to interference. As expected, reactive control performance remained intact with aging (similar interference effect in the two groups). In contrast, we observed a larger interference effect in the proactive condition in aging. Finally, older participants with similar level of fluid intelligence to young adults showed no proactive control age-related decrement. Beyond the fact that this study confirms the selective age-related decline in proactive control, it also indicates that the level of fluid intelligence influences the efficiency of proactive control in aging. [less ▲]

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See detailCurrent Pericopsis elata (Fabaceae) patches in southeastern Cameroon: remnants of a long and rich Human-rainforest relationship?
Bourland, Nils ULiege; Cerisier, François; Livingstone Smith, Alexandre et al

Poster (2012, June 23)

Pericopsis elata is one of the most valuable African timber species. This IUCN Red Listed tree suffers from a lack of regeneration, thus its current presence provokes questioning. Our work aimed at ... [more ▼]

Pericopsis elata is one of the most valuable African timber species. This IUCN Red Listed tree suffers from a lack of regeneration, thus its current presence provokes questioning. Our work aimed at understanding its origins so as to help securing its future. This study, lead away from engineering works, was conducted in four different sites located within the natural distribution area of the species and taking into account the different growing conditions were the species occurs. Our observations are based on an analysis of charcoal elements and pottery fragments discovered in subsurface layers of soils as well as current botanical and pedological surveys. Evidence of past human activities we found led to the assumption that this part of the Congo Basin was much more inhabited than previously thought. Some of the results obtained for P. elata could apply for other long lived light demanding species growing in the same environment [less ▲]

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See detailRole of γδ T cells in HPV-induced cancer progression
Van hede, Dorien ULiege; Bastin, Renaud; Francis, Floriane et al

Poster (2012, June 22)

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See detailCheckpoints modulation by the human T-lymphotropic virus type 1 (HTLV-1) Tax protein : towards new therapeutic approaches
Carpentier, Alexandre ULiege; Willems, Luc

Poster (2012, June 22)

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is ... [more ▼]

HTLV-1 infects approximately 20 million people worldwide and causes several diseases. This virus is responsible for the adult T-cell leukemia (ATL) and for a chronic neuropathology (TSP/HAM). There is currently no satisfactory treatment for these diseases. Among the proteins encoded by HTLV-1, Tax appears to play an important role in the mechanisms leading to pathogenicity. We are interested in the mechanisms of cell transformation by HTLV-1 and more particularly in the interplay between the viral Tax oncoprotein and the DNA damage response (DDR). We demonstrated that transient expression of Tax results in DNA damage, cell cycle arrest and activation of the ATM-Chk2-p53 axis of the DDR. In fibroblasts, cell cycle arrest occurs at the G1 and G2 phases depending on the p53 background. In contrast, HTLV-1 infected lymphocytes proliferate continuously and appear to be adapted to Chk2 and p53 checkpoints. This mechanism allows infected lymphocytes to proliferate despite the presence of genomic lesions. Our data shows that HTLV-1 infected cells use an alternative DNA repair pathway controlled by ATM and Chk1. This particularity may be used as novel therapeutic approach based on the principle of synthetic lethality. [less ▲]

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See detailOncogenic human papillomavirus could directly interact with Natural Killer cells
Renoux, Virginie; Bastin, Renaud ULiege; Boniver, Jacques ULiege et al

Poster (2012, June 22)

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See detailInteraction of HTLV-1 Tax with minichromosome maintenance proteins accelerates the replication timing program
Barez, Pierre-Yves ULiege; Willems, Luc ULiege

Poster (2012, June 22)

First human retrovirus discovered, HTLV-1 infects approximately twenty million individuals worldwide. HTLV-1 is the causative agent of different diseases among which the most important are the adult T ... [more ▼]

First human retrovirus discovered, HTLV-1 infects approximately twenty million individuals worldwide. HTLV-1 is the causative agent of different diseases among which the most important are the adult T-cell leukemia (ATL) and a neurodegenerative disorder called HAM/TSP (Human associated myelopathy/ Tropical spastic paraparesis). We are interested in the mechanisms of transformation by the viral Tax oncoprotein. Recently, we showed that Tax interacts with the minichromosome maintenance MCM2-7 helicase and binds to origins of DNA replication (Boxus et al, 2012 Blood 119:151). Thereby, Tax modulates the spatiotemporal program of origin activation during the S phase of cell cycle. In fact, Tax accelerates S phase progression by inducing early firing of late replication origins. Concomitantly, Tax-MCM2-7 interplay also modulates viral transcription. Together, our data demonstrates that interaction between Tax and MCM2-7 is involved in replication reprogramming and viral transcription. [less ▲]

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See detailMolecular imaging through in combinaison with quantitative proteomic approaches unraveling the molecular players of breast cancer adaptation to anti-angiogenic therapy.
Cimino, Jonathan ULiege; Sounni, Nor Eddine ULiege; Calligaris, David ULiege et al

Poster (2012, June 22)

Breast carcinoma is the most common and second leading cause of cancer mortality in women. The recognition of the “angiogenic switch” as a rate-limiting secondary step in tumorigenesis led to extensive ... [more ▼]

Breast carcinoma is the most common and second leading cause of cancer mortality in women. The recognition of the “angiogenic switch” as a rate-limiting secondary step in tumorigenesis led to extensive pre-clinical researches on angiogenesis and finally the approval of VEGF-neutralizing antibodies (bevacizumab) and VEGF receptor tyrosine kinase inhibitors (RTKs:Sunitinib). The Sunitinib has been used clinically in patients with breast cancer refractory to other therapeutic agents. Unfortunately, like the cytotoxic therapies, these drugs do not produce lasting effects and resistance to treatment appeared clinically. Questions have emerged about the failure of anti-angiogenic therapy in clinic and the limitations of predictive preclinical models, and also about the molecular assessment of all stages of tumor adaptation and metastatic disease. To this end, we applied quantitative proteomics and imaging mass spectrometry tools to visualize and study the profiles of proteins and small molecules associated with tumor treated or not with Sunitinib using a novel preclinical model of breast carcinoma cells. In this project, we first developed a reproducible model of resistance to Sunitinib of human triple negative breast cancer MDA-MB-231 cells expressing luciferase gene. Cells were subcutaneously injected into mice RAG1-/- and divided into four experimental groups including, control mice treated with vehicle or Sunitinib for 30 days and sacrificed 1 days after treatment withdrawal or when tumor reached a volume of 300 mm3. In the second step. Tumors were analyzed using a nanoAcquity UPLC Synapt TM HDMS TM G1 (Waters, Manchester,UK) and Mass Spectrometry Imaging. For quantitative proteomic analyses of tumors, a bioinformatics analysis was used with the Protein lynx global server 2.2.5 software. Imaging mass spectrometry was performed on tissue sections of tumors and organs subsequently colonized by metastases. Matrix sublimation was used to coat tumor sections (14 µm-tick) with 1.5 Diaminonaphthalene for lipids analysis and Sinapinic acid for entire proteins analysis. Ion cartographies were recorded with a Solarix 9.4T FTMS instrument for lipids and with an Ultraflex II TOF-TOF instrument for entire proteins (Bruker Daltonics, Germany) with a spatial resolution of 100 µm. Global protemic revealed different protein profiles between tumor treated or not with Sunitinib. The Mass Spectrometry Imaging detected differences in intensity and location of some proteins and lipids are also associated with some histological features including inflammatory, necrotic and angiogenic areas. Bioinformatics analysis will be applied to ensure the integration of all data in order to provide the basis for identifying molecular pathways activated during the acquisition of refractoriness to drug treatments. [less ▲]

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See detailSelection and cultivation of hydrolytic microorganisms extracted from the digestive tract of the termite Reticulitermes santonensis (3DV.1.55)
Tarayre, Cédric ULiege; Bauwens, Julien ULiege; Matteotti, Christel ULiege et al

Poster (2012, June 21)

Biofuel production can be based on the use of fermentable substrates issued from the hydrolysis of lignocellulosic biomass stemming from agricultural residues and by-products. However, such substrates are ... [more ▼]

Biofuel production can be based on the use of fermentable substrates issued from the hydrolysis of lignocellulosic biomass stemming from agricultural residues and by-products. However, such substrates are not easy to degrade. Enzymes (cellulases, xylanases, etc.) can be used for this purpose and pre-treatments can increase their action by providing more available extremities. The digestive tract of the termite Reticulitermes santonensis contains various microorganisms (bacteria, molds, protists) able to degrade the wood components. These microorganisms act as consortia, leading to a better hydrolysis than in the cow rumen. Our purpose is the isolation of microorganisms from termite guts in order to evaluate their potential for hydrolysis of lignocellulosic materials. This approach led us to isolate and to study a bacteria (Bacillus sp.) displaying a xylanase activity, a mold (Aspergillus sp.) displaying a cellulase activity and a chrysophyte (protist) displaying an amylase activity. [less ▲]

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See detailPericopsis elata (Harms) Meeuwen in Cameroon: Ecological Check-up of an Endangered Timber Species
Bourland, Nils ULiege; Kouadio, Yao Lambert; Lejeune, Philippe ULiege et al

Poster (2012, June 20)

Pericopsis elata (Fabaceae – assamela, afrormosia, kokrodua) is a high valued timber species of the moist semi deciduous African forests. Because of logging which started more than 50 years ago, it is ... [more ▼]

Pericopsis elata (Fabaceae – assamela, afrormosia, kokrodua) is a high valued timber species of the moist semi deciduous African forests. Because of logging which started more than 50 years ago, it is considered as threatened and included on both IUCN Red List and CITES Appendix II. Nevertheless, there is still little information available on the species ecology: essential biological parameters controlling its population dynamics remain unknown. Our study first aims at improving the knowledge of its main ecological parameters, then at assessing the impact of selective logging on its populations in a forest management unit in Cameroon (ca 120,000 ha). After inventorying the species (sampling rate of 1.2%), mortality and growth were assessed over continuous 5 and 2-year periods in unlogged and logged areas, respectively. Phenology was monitored in the unlogged forest during 5 years (leaf shedding and flushing, flowering, ripe and unripe fruiting). The population structure followed a bell-shaped curve. Mean annual diameter increments in both environments did not differ significantly between unlogged and logged areas (0.29±0.06 0.31±0.04 cm for unlogged and logged areas, respectively). P. elata is a deciduous species that flowers at the end of the main dry season (Marsh-April). The minimum reproduction and effective flowering diameters were, respectively, 32 and 37 cm. Fruit maturation took place during 7 months (the seed rain occurs in December-January), but all unripe fruits abort 3 years out of 5. With a minimum logging diameter of 90 cm, the recovery rate computed over a 30-year period was greater than 100%. Selective logging harvested only 12.1% of the total number of seed trees and had little influence on the species biological parameters. Securing sufficient regeneration as a post-logging action is probably the most important consideration for achieving long-term sustainability. [less ▲]

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See detailAre ecological indices based on macrophytes communities pertinent tools to monitor ecological potential of Walloon reservoirs?
Sossey Alaoui, Khadija ULiege; Galoux Daniel, SPW; Rosillon, Francis ULiege

Poster (2012, June 19)

Diversity and trophic metrics based on macrophyte communities were calculated to test their pertinence to assess the ecological potential of Walloon reservoirs (Belgium). The BE-FL method (four ... [more ▼]

Diversity and trophic metrics based on macrophyte communities were calculated to test their pertinence to assess the ecological potential of Walloon reservoirs (Belgium). The BE-FL method (four complementary metrics) and the United Kingdom method (five metrics calculated using information on taxonomic composition and abundance of macrophyte species and groups of such species) were tested. Highly significant correlations were found between the chemical variables (o-P (mg P/l); P tot (µg P/l); NH4+ (mg N/l) DCO (mg/l) Cond (µS/cm), the phytoplankton assemblage index, Q (Padisak et al., 2006) applied for the same reservoirs (Descy et al., 2010). and trophic metric LMNI and between them and the abundance of disturbance indicators (V). Groupe of macrfophyte species metrics do not necessarily provide any direct information on the quality or degree of degradation of the environment from which the sample was taken, whereas trophic indices do. [less ▲]

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See detailAnalysis of the allergenicity of natural and recombinant Der p 3
Bouaziz, Ahlem ULiege; Campisi, vincent; Herman, julie et al

Poster (2012, June 19)

Background: Der p 3 a trypsin-like protease is a Dermatophagoides pteronyssinus allergen which is synthesized in the mite under a zymogen form. The enzymatic activity of this allergen has been shown to ... [more ▼]

Background: Der p 3 a trypsin-like protease is a Dermatophagoides pteronyssinus allergen which is synthesized in the mite under a zymogen form. The enzymatic activity of this allergen has been shown to enhance the inflammatory process of allergy. To date, there are a few studies that described the allergenicity and the IgE reactivity of the group 3 allergens, the allergenic properties of recombinant Der p 3 was also not characterized. Methods: The autolysis of rDer p 3 and rDer p 3 S196A were analyzed by means of SDS-PAGE and enzymatic activity and their allergenicities by means of assays for IgE binding, IgE binding inhibition and basophiles mediator-release. Results: 100% of the sera from allergic patients showed IgE reactivity to natural Der p 3 and recombinant form. However, the IgE binding to the Der p 3 was less 4 times than rDer p 1. The IgE binding to rDer p 3 S196A was higher than rDer p 3. These variations can be linked with the phenomen of autolysis and instability of Der p 3 during the ELISA test. The mediators release performed with RBL sensitized with sera from allergic patients and stimulated with rDer p3, rDer p 3 S196A and natural Der p 3 were similar and lower than Der p 1 for concentration < 10 ng/ml. Conclusions: recombinant mature Der p 3 retained overall identity to its natural form in terms of structure and allergenicity. The instability and autolysis of Der p 3 drastically influence its IgE binding capacity. These results can be explain the considerable variations with the frequencies reactivity (16-100%) of natural Der p 3. The RBL assays demonstrated that the allergenicity of rDer p 3 is similar to rDer p 1 for the concentrations >10 ng /ml. [less ▲]

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See detailGenetic parameters for methane indicator traits based on milk fatty acids in Dual Purpose Belgian Blue cattle
Kandel, Purna Bhadra ULiege; Gengler, Nicolas ULiege; Soyeurt, Hélène ULiege

Poster (2012, June 18)

Dairy production is pointed out for its large methane emission. Therefore, specific nutritional strategies are applying to abate methane emission but very less information is available about the animal ... [more ▼]

Dairy production is pointed out for its large methane emission. Therefore, specific nutritional strategies are applying to abate methane emission but very less information is available about the animal genetic variability of methane emission. Methane indicators using traits indirectly related to methane and easily recorded like the mid-infrared (MIR) prediction of fatty acid could be used to conduct genetic studies. MIR methane indicators used in this study were derived from published fatty acid based methane indicators using 597 calibration samples. Genetic parameters of these MIR indicators were estimated by single trait random regression test-day models from 13,389 records collected on 1602 Dual Purpose Belgium Blue cows in their first 3 lactations. For the published indicator showing the highest relationship (R2 =0.88) with Sulphur hexafluoride (SF6) methane emission data, the average daily heritability was 0.25±0.06, 0.25±0.07 and 0.18±0.09 for the first three lactations, respectively. Similarly, the lactation heritability was 0.45±0.09, 0.46±0.11 and 0.24±0.14. The sire genetic variability was 3.60, 4.08, 1.19 kg2 of methane for the first three lactation, respectively. The genetic difference between the sires having cows eructing the highest and the lowest methane content was 11.62, 13.01 and 5.98 kg per lactation for the first three parities. This study suggested that methane indicator traits can be predicted by MIR and the genetic variability of these traits seems to exist. Therefore, it also suggests the genetic variability of methane content eructed by dairy cows. These first finding might open new opportunities for animal selection program on methane emission. [less ▲]

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See detailmulti-technique analysis of electrochemical mechanisms in tin based intermetallic anodes
Lippens, Pierre-Emmanuel; Chamas, Mohamad; Sougrati, Moulay Tahar et al

Poster (2012, June 17)

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See detailMetabolic plasticity of wild-type and AOX-deficient Chlamydomonas reinhardtii cells related to the inorganic nitrogen source (nitrate or ammonium), as revealed by a 2D-DIGE comparative proteomic analysis
Gerin, Stéphanie ULiege; Mathy, Grégory; Franck, Fabrice ULiege

Poster (2012, June 15)

In the model unicellular green alga Chlamydomonas reinhardtii, both nitrate and ammonium can be used as primary inorganic nitrogen sources. Interestingly, the expression of the mitochondrial alternative ... [more ▼]

In the model unicellular green alga Chlamydomonas reinhardtii, both nitrate and ammonium can be used as primary inorganic nitrogen sources. Interestingly, the expression of the mitochondrial alternative oxidase (AOX), an "energy-dissipating" ubiquinol-oxygen oxidoreductase of the mitochondrial electron transport chain, is under the control of the exogenous nitrogen source : it is activated in nitrate-grown cells and repressed in ammonium-grown cells at both transcriptional and translational levels. This regulation of AOX by nitrogen is Chlamydomonas-specific and currently its bioenergetic and metabolic significance is poorly understood. In order to get clues to this peculiar phenomenon, we characterized the global metabolic response of a wild-type strain (WT) and an AOX-deficient mutant (AOX-) obtained by RNA interference grown either on nitrate or ammonium. For this purpose, we used a highly accurate 2D electrophoresis-based comparative proteomic approach (2D-DIGE) to compare the cellular proteomes of nitrate and ammonium-grown WT and AOX- Chlamydomonas. The analysis was performed in the middle of the exponential growth phase in mixotrophic conditions. It revealed many proteomic modifications between WT and AOX- cells and a smaller number between nitrate and ammonium-grown cells. In nitrate-grown cells, we notably observed an important up-regulation of glutamine synthetase. Interestingly, in AOX- cells, we respectively detected a general down-regulation and a general up-regulation of mitochondrial and chloroplastic bioenergetic enzymes, and also an important up-regulation of glutathione-dependent oxidative stress defense systems together with a remarkable down-regulation of methionine synthase. Altogether these results and previous studies provide new features in understanding the metabolic adaptations occurring in response to the inorganic nitrogen source with emphasis on the role played by AOX. [less ▲]

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See detailOptimization of an Haematococcus pluvialis medium by a Genetic Algorithm-based strategy
Fratamico, Anthony ULiege; Tocquin, Pierre ULiege; Franck, Fabrice ULiege

Poster (2012, June 14)

The successful use of living organisms for the production of biomass or metabolites requires a careful control and optimization over growing conditions. However the range of interacting parameters makes ... [more ▼]

The successful use of living organisms for the production of biomass or metabolites requires a careful control and optimization over growing conditions. However the range of interacting parameters makes full optimization difficult and time-consuming. In this context, Genetic Algorithm-based (GA) methods emerge as promising strategies for optimization of biotechnological processes. However, the potential of GA in the microalgae field remains, today, poorly explored. As a proof-of-concept, we evaluated how GA could be applied for the optimization of a medium for high yield photoautotrophic growth of Haematoccocus pluvialis. [less ▲]

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See detailPRoNTo: Pattern Recognition for Neuroimaging Toolbox
Schrouff, Jessica ULiege; Rosa, Maria J; Rondina, Jane et al

Poster (2012, June 12)

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See detailSelf-appraisal and medial prefrontal activation in early stage Alzheimer’s disease
Genon, Sarah ULiege; Collette, Fabienne ULiege; Angel, Lucie et al

Poster (2012, June 12)

Introduction Self-referential processing in healthy subjects is related to activation within cortical midline structures, such as the medial prefrontal cortex (MPFC) and the posterior cingulate cortex ... [more ▼]

Introduction Self-referential processing in healthy subjects is related to activation within cortical midline structures, such as the medial prefrontal cortex (MPFC) and the posterior cingulate cortex (PCC; Northoff et al., 2006). Little is know about the engagement of these structures during self-referential processing at different stages of Alzheimer’s disease (AD). The PCC and the MPFC have been found to be activated during a self-appraisal task of adjectives in patients at very early stage of the disease (patients with Mild Cognitive Impairment, MCI; Ries et al., 2006). In contrast, in a similar task, Ruby et al. (2009) have found that mild demented patients activated the dorsal part of the MPFC (DMPFC) to a lesser extent than healthy controls (HC). Ruby et al. did not assess depression symptoms in their patients. Yet, MPFC activations have been found to be modified during self-referential processing in depressed participants (Lemogne et al., 2012). Therefore, in this study, we examined brain correlates of self-appraisal processing in AD patients when controlling for depressive symptoms. Methods Twenty-two mild AD patients and 22 HC matched on age, level of education and gender (respectively: 76±5y; 11±3y; 12M10F) to the AD patients (respectively: 76±7y; 11±3y; 11M11F) were recruited. To control for dementia severity and depression, the participants were administrated the Mattis Dementia Rating (MDR) and the Geriatric Depression Scale (GDS). A self-appraisal task intermixed with a recognition task was administered in an fMRI experimentation. In the self-appraisal task, the participants saw adjectives and had to indicate if the trait describes them (Self-condition; SC) or the King Albert II (for men)/the Queen Fabiola (for women; Other-condition; OC). The adjectives were presented in blocks of 6 items. Participants performed 9 runs consisting in one block of SC and one block of OC followed by a recognition phase where participants were presented with the 12 adjectives that they had just previously seen randomly mixed with 12 new adjectives. They were asked to indicate for each adjective whether they had seen it in the previous task. Statistical analyses focused on the self-appraisal task. Brain activations related to the self appraisal process were isolated in each participant by subtracting brain activation related to OC items from brain activation related to SC items. Then at the group level, we examined differences between groups (HC>AD and AD>HC) and a conjunction analysis examined brain activations that were common to both groups. Preprocessing and statistical analyses were performed with SPM8 (p<.001 uncorrected with a-priori hypotheses). Results GDS scores were similar in AD (3±3) and HC (3±3; T(42) = .1; P=.9) groups. No region was found to be significantly more activated during self-appraisal process in HC than in AD and vice versa when performing direct statistical comparison. Moreover, a conjunction analysis revealed that the VMPFC was the only region commonly activated in AD and HC during self-appraisal process (Punc<.001). Conclusions Our results revealed that AD patients engaged the ventral part of the MPFC to a similar extent than HC during self-appraisal judgements. These results and the results found in patients with MCI by Ries et al. (2006) suggest that at initial stages of AD, patients engaged self-related regions when they performed judgements about themselves as HC do. The divergence with the findings by Ruby et al. (2009) may be related either to the fact that they did not controlled for depressive symptoms or to the fact that their patients showed on average lower scores at the MDR (124) than our patients (127). One can assume that engagement of the self-related regions during self-appraisal judgements in the AD patients depends on the severity of the dementia and/or depressive symptoms. In conclusion, MPFC may be engaged during self-referential processing in very mild AD patients without depressive symptoms. References: Lemogne, C., Delaveau, P., Freton, M., Guionnet, S. & Fossati, P. (2012). Medial prefrontal cortex and the self in major depression. Journal of Affective Disorders, 136, 1-11. Ries, M. L., Schmitz, T.W., Kawahara, T.N., Torgerson, B.M., Trivedi, M.A. & Johnson, S.C. (2006). Task-dependent posterior cingulated activation in mild cognitive impairment. NeuroImage, 29, 485-492. Ruby, P., Collette, F., D’Argembeau, A., Péters, F., Degueldre, C., Balteau, E., Luxen, A., Maquet, P. & Salmon, E. (2009). Perspective taking to assess self-personality: What’s modified in Alzheimer’s disease ? Neurobiology of Aging, 30(10), 1637-1651. [less ▲]

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See detailIn vitro determination of prebiotic potential of sugar beet pulp extracted candidates and influence of production method
François, Emmanuelle ULiege; Goffin, Dorothée ULiege; combo, Agnan Marie-Michelle et al

Poster (2012, June 12)

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol ... [more ▼]

Various prebiotic hydrolysates were produced starting from sugar beet pulp (SBP) using alternative solution to traditional acidic hydolysis. SBP pectin was first extracted by acid extraction and ethanol precipitation. Then two technics are used to produced pectic oligosaccharides (POS) : enzymatic hydrolysis (EnzPOS : Rapidase Smart (DSM) ; 50°C ; pH 5) and microwaves-assisted hydrolysis (MW1POS : 104°C/10min/pH 7; MW2POS : 110°C/15min/pH 7). Structural characterization of fractions gave rise to different structural profiles between the four products. Moreover, fermentation parameters obtained in-vitro (A, B, Rmax and Tmax) were also characteristic of the fractions and in favor of a tight relationship between POS structure and POS function. Finally, to avoid solvent use, POS production could be envisaged directly on SBP. Avoiding the step of acidic extraction will allow to meet the green chemistry principles. [less ▲]

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See detailAutomatic multiclass classification of 18FDG-PET scans for the distinction between Parkinson’s disease and atypical parkinsonian syndromes
Phillips, Christophe ULiege; Schrouff, Jessica ULiege; Luxen, André ULiege et al

Poster (2012, June 10)

Part of the difficulty in the early diagnosis of Parkinson’s disease (PD) is in differentiating it from atypical parkinsonian disorders (APS) that have a poorer prognosis such as multiple system atrophy ... [more ▼]

Part of the difficulty in the early diagnosis of Parkinson’s disease (PD) is in differentiating it from atypical parkinsonian disorders (APS) that have a poorer prognosis such as multiple system atrophy (MSA), progressive supranuclear palsy (PSP) and corticobasal syndrome (CBS). 18flurodeoxyglucose (FDG) positron emission tomography (PET) has been recommended for the early differentiation between PD and APS [1]. Here, 120 FDG PET scans (42, 31, 26 and 21 for the PD, MSA, PSP and CBS resp.) were acquired on average 3.5 years after symptoms onset (because the initial clinical features were outside the prevailing perception for PD) to look, without any a priori assumption, for cerebral FDG uptake patterns that discriminate either between the PD and APS classes, or between the four PD, MSA, PSP and CBS classes. The diagnostic used to label the scans was defined by clinical criteria on average 4.5 years after PET assessment. [less ▲]

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See detailIncrease in cortico-thalamo-cortical connectivity during human sleep slow wave activity
Kussé, Caroline ULiege; Lehembre, Rémy; Foret, Ariane et al

Poster (2012, June 10)

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See detailCrystal Structure and Local Disorder in Modern and Ancient Prussian Blue Pigments
Samain, Louise ULiege; Martinetto, Pauline; Bordet, Pierre et al

Poster (2012, June 06)

The necessity of understanding degradation and alteration processes in a painting's materials is well established for preservation and art history issues. The task is however complex because of the highly ... [more ▼]

The necessity of understanding degradation and alteration processes in a painting's materials is well established for preservation and art history issues. The task is however complex because of the highly heterogeneous character of a paint layer, which consists of a mixture of pigments and a binder on a support. In this context we focus on a particular pigment, Prussian blue. Prussian blue is a hydrated ferric ferrocyanide complex, first synthesized in 1704 in Berlin. It has been widely used by artists until the 1970's. However reports of discoloration had already appeared in eighteenth and nineteenth century books. To date, little attention has been devoted to the understanding of the degradation processes of Prussian blue in paint layers. The preparation methods of Prussian blue were rapidly recognized as a contributory factor in the fading of the pigment because they lead to the introduction of impurities in its structure. The crystal structure of Prussian blue is notoriously complex because of the presence of vacancies and local disorder. Unresolved questions about the crystal structure of the soluble variety of Prussian blue, i.e., Prussian blue containing alkali cations, are still found in the literature. We reproduced modern and ancient preparation methods of Prussian blue and analyzed the obtained pigments by high-energy powder diffraction at the beamline ID11, ESRF, Grenoble and at the beamline CRISTAL, Soleil, Paris. The crystal structure of soluble Prussian blue was reviewed by Rietveld refinement and appears to contain approximately a quart of iron(II) sites vacant, similarly to the well-known insoluble crystal structure. The refinement of the pair distribution function extracted from the total scattering signal revealed a local structure different from the average one. The local arrangements are best described by combining three different substructures with different numbers of vacancies and vary upon the type of synthesis. The PDF analysis also evidenced the formation of nanocrystalline ferrihydrite and alumina hydrate in Prussian blue pigments synthesized according to eighteenth-century recipes. The local disorder and the presence of an undesirable iron compound in Prussian blue can help to better understand the degradation mechanisms in paint layers containing this pigment. [less ▲]

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See detailMechanochemical Study of Conformational Transitions in a Single Synthetic Peptide Chain
Willet, Nicolas ULiege; Hinterdorfer, Peter; Lecommandoux, Sébastien et al

Poster (2012, June 05)

The aim of this study is to investigate the mechanochemical behavior of homopolypeptides able to change their conformation is a stimuli-responsive way. The peptidic secondary structures were studied in ... [more ▼]

The aim of this study is to investigate the mechanochemical behavior of homopolypeptides able to change their conformation is a stimuli-responsive way. The peptidic secondary structures were studied in detail by atomic force microscopy (AFM) at the single-molecule level. Synthetic copolymers containing a polypeptide block were prepared by N-carboxyanhydride amino acid ring-opening polymerization. The polymer chains were grafted as a dilute brush onto gold surfaces via disulfide end-groups. Their mechanochemical behavior was then studied by AFM single-molecule force spectroscopy (SMFS). The investigated polypeptide blocks were based on poly(L-glutamic acid), which undergoes a transition from alpha-helix to random coil. This can be induced by external stimuli (pH, ionic strength, temperature) or simply by applying a force. We were able to study the mechanically driven unfolding of the peptide by stretching-release cycles of the biomacromolecule. Stretching the helical peptide resulted in original features in the force-distance traces. Plateaus that are specific for the helical conformation were detected, quantified and discussed. Pulling-relaxing SMFS experiments eventually led to a better understanding of the force induced unfolding of an alpha-helix and the reversibility of the phenomenon. [less ▲]

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See detailUse of glycosurfactants for the synthesis of polyHIPES in supercritical carbon dioxide
Boyère, Cédric ULiege; Favrelle, Audrey; Léonard, Alexandre ULiege et al

Poster (2012, June 04)

The abstract is available as a pdf file.

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See detailThe MicroH2 project:an association of four laboratories to improve theknowledge on biohydrogen production precesses
Beckers, Laurent ULiege; Calusinska, Magdalena ULiege; Hamilton, Christopher ULiege et al

Poster (2012, June 04)

This poster presents a collaborative research project (MicroH2) held at the University of Liège (Belgium) since 2007 (www.microh2.ulg.ac.be) and involving four different research groups. The project aims ... [more ▼]

This poster presents a collaborative research project (MicroH2) held at the University of Liège (Belgium) since 2007 (www.microh2.ulg.ac.be) and involving four different research groups. The project aims to develop a center of excellence in the fields of photo- and dark- biohydrogen production. Our studies contribute to improve the knowledge of the processes involved in the microbiological production of hydrogen, from a fundamental and practical point of view. Some results are highlighted here. The research concerning photofermentation focuses on the interactions between respiration, photosynthesis and H2-producing pathways in algal microorganisms, by using mitochondrial mutants and genetically modified strains with modified ability for hydrogen production [1-2]. To study the metabolism of the hydrogen production by anaerobic bacteria, pure cultures and defined consortia are used and their production of biogas and soluble metabolites is measured. Moreover, we have developed and optimized molecular tools, like quantitative RT-PCR and FISH, to monitor the variations of bacterial populations in novel bioreactors for hydrogen production [3-4]. We have also mined the complete genomes of Clostridium spp. for putative hydrogenase genes and found a large diversity of them [5]. [less ▲]

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See detailDiscrimination of pure grassland species using NIR Hyperspectral Imaging
Dale, Laura ULiege; Bogdan, Anca Dorina; Pacurar, Florin Simion et al

Poster (2012, June 03)

The objective of this study was to discriminate by hyperspectral imaging system, SWIR ImSpector N25E, different pure grassland species (Festuca rubra L., Trifolium repens L., Agrostis capillaris L ... [more ▼]

The objective of this study was to discriminate by hyperspectral imaging system, SWIR ImSpector N25E, different pure grassland species (Festuca rubra L., Trifolium repens L., Agrostis capillaris L., Hieracium aurantiacum L., Arnica montana L.) into grassland species mixtures. All the samples were collected from natural meadows of the National Apuseni Park, Apuseni Mountains, Gârda area (Romania). The samples were air-dried, then prepared using the protocol for NIRS analysis adapted on the scanning linear system. For images acquisition, the Hyper See program was used. Then a model build under MatLab (PLS–DA) was used to discriminate pure species from the mixtures of two or three species. This analysis was carried out in order to see, on images obtained previously from the floristic composition of experimental parcels, if the pure species are or are not recognized according to the spectral data base. More than 99% correct predictions for species discrimination were obtained. This study should guide us to verify if a toxic species is present or not in natural meadows used as food for animals. The floristic composition of a meadow can be determinate only if we have in the data base, spectra for each identified species, as being part of the mixture. [less ▲]

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See detailRaymond Lemaire and the Historic City
Houbart, Claudine ULiege

Poster (2012, June 02)

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See detailComparison of mineral intake between children from endemic and non-endemic areas for Kashin-Beck disease in Tibet Autonomous Region: Pilote study
DERMIENCE, Michael ULiege; Maesen, Philippe ULiege; Mathieu, Françoise et al

Poster (2012, June 01)

Background The Kashin-Beck disease (KBD) is an endemic and chronic osteochondropathy affecting between 0.74 million and 2.5 million people in the Tibet Autonomous Region and in several provinces of the ... [more ▼]

Background The Kashin-Beck disease (KBD) is an endemic and chronic osteochondropathy affecting between 0.74 million and 2.5 million people in the Tibet Autonomous Region and in several provinces of the People’s Republic of China. The etiology remains unclear, although a multifactorial hypothesis has been proposed (selenium/iodine deficiency; high concentration of organic matters in drinking water; and mycotoxin poisoning by fungi infecting cereals). The rural population is almost exclusively affected. Objectives The first objective of this study was to assess and to compare the mineral daily intake between Tibetan preschool children living in endemic areas for the Kashin-Beck disease and those living in non-endemic areas. A second objective was the comparison of children daily intakes with Chinese Dietary Reference Intakes (DRIs). The third objective was to estimate a sample size for a cross-sectional survey aiming to highlight significant differences in mineral intakes between the two groups of preschool children. Materials and Methods Ten Tibetan preschool children were enrolled per group (endemic/non-endemic) for this pilot study. Children had to be weaned and those living in endemic area must have a KBD sibling. The nutrition survey consisted in an interactive simplified 24-hour recall questionnaire. Two 24-hour recalls were recorded for the endemic group, in February 2010 (EAw) and in May 2010 (EAs), while one day has been recorded for the non-endemic group in July 2011 (NEA). At the same time, samples of the main staple foods were collected for chemical analysis. The daily intakes of Ca, P, Mg, Fe, Zn, Cu, and Mn were calculated based on the 24-hour recall records, the chemical analyses, and data from food composition tables (FCTs). Results and discussion We are aware of limitations in the methodological approach of this pilot-study. The number of children, although weak, was empirically decided for the sake of feasibility and because no prior information was available. The interactive 24-hour recalls between the two groups are spaced in time. Nevertheless, the diet of rural Tibetans is far from diversified, and the variability among seasons and years is probably low. Daily intakes in Ca, P, Mg, Fe, Zn, Cu and Mn were calculated for children of each group. Parametric test were applied for comparison of mean daily intakes between groups. A significant difference was highlighted for Cu (p-value<0.01) and Fe (p-value<0.05), no other significant difference was detected. When comparing individual daily intakes and Chinese DRIs, the most striking results concern Ca and Mn. The great majority of children showed severe deficiency in calcium and enormous excess in manganese. A power (1-β) of 80% is commonly accepted when estimating a sample size for a cross-sectional survey. In this case, the maximum sample size is too high and not feasible in practice (several hundred children per group). Oppositely, the power was calculated per mineral, based on 100 children per group. The power is higher than 90% for Mg, Fe, and Cu. For the others minerals, the power is well below 80%. Nevertheless, general deficiencies and excesses have been observed in the two groups for part of these minerals. We believe that it is a statement in itself and trying to highlight small differences between groups in such extremes is not relevant. [less ▲]

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See detailEffect of air-drying conditions on physical and nutritional properties of osmotically pre-treated pomegranate seeds
Bchir, Brahim ULiege; Besbes, Souhail; Karoui, Romdhane et al

Poster (2012, June 01)

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See detailSpecialized « ochre » procurement strategies in the Transition context : the red pigments from the Châtelperronian of the Grotte du Renne, Arcy-sur-Cure (France)
Salomon, Hélène ULiege; Coquinot, Yvan; Beck, Lucile et al

Poster (2012, June 01)

In many reports of prehistoric pigment studies, these artefacts are considered as the testimony of past symbolic activities. The first step of the processing sequence, that is to say the acquisition of ... [more ▼]

In many reports of prehistoric pigment studies, these artefacts are considered as the testimony of past symbolic activities. The first step of the processing sequence, that is to say the acquisition of raw colouring material, is not well described and understood. Physico-chemical (SEM-EDS, XRD, TEM-EDX, µPIXE-µPIGE) and petrological analysis were carried out on the colouring materials excavated in the châtelperronian layers (40000-35000 B.P.) of the French site the Grotte du Renne in Arcy-sur-Cure. The Châtelperronian is one of the transitional techno-complexes, basically one of the last cultures made by Neanderthals in Europe. The physico-chemical data were related to the location of the colouring materials on the site, in association with exceptionally well preserved “hut” structures. It was thus possible to demonstrate that none of these materials, either red or black, was heated before being used, contrary to what had been assumed so far. The supply in colouring materials was as carefully organized as for mineral materials such as flint, for example; they were collected in different geological formations occasionally showing on the surface, close to the cave and at more than 30 km from the cave. The exploitation of these geological sites did not vary during the whole Châtelperronian period, and privileged materials which can easily be ground to powder. The set of colouring minerals from the Grotte du Renne reveals Neanderthals’ in-depth knowledge of mineral materials; they understood perfectly well their properties and qualities, and used them extensively, so that the raw colouring material was part of the livelihood and the Châtelperronian site must have been a literally dazzling sight, all red and black. [less ▲]

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See detailLa communication du risque au sein de la sécurité alimentaire : la gouvernance des risques avec un grain de BPA
Vanhaeren, Stéphanie ULiege

Poster (2012, June 01)

Lors d’une réflexion sur les politiques modernes de sécurité alimentaire, trois modèles principaux d’évaluation de politiques publiques sont en compétition : les modèles de risque technocratique ... [more ▼]

Lors d’une réflexion sur les politiques modernes de sécurité alimentaire, trois modèles principaux d’évaluation de politiques publiques sont en compétition : les modèles de risque technocratique, décisionniste et « transparent » (Millstone 2004). Chacun de ces modèles amène de nouveaux acteurs dans le processus décisionnel : dans le modèle technocratique, les scientifiques sont la seule source d’information pour les décideurs politiques. Les acteurs socio-politiques et économiques sont invités à la table de discussion dans le modèle décisionniste, quoiqu'une moindre importance soit donnée à leur avis. Enfin, le modèle transparent plaide pour que le poids des avis de chacun de ces acteurs soit égal. Ce dernier modèle a inspiré celui de la gouvernance des risques de Renn (Dreyer and Renn, 2009), qui complète le modèle transparent en plaçant une nouvelle emphase sur l'évaluation et la communication du risque. Cette communication est un concept central dans l’évaluation des politiques publiques (Muller, 2005), qui considère la politique comme le résultat d’interactions entre un nombre important d’acteurs, chacun apportant ses propres vues du monde et approches de l’action. Le but de ces politiques est de définir des modes d’action et de représentation de la réalité qui guideront les choix et actions mises en place pour résoudre des problèmes communs et connus. Muller (2005) appelle cette construction sociale « référentiel sectoriel d’action publique ». Le référentiel résulte d’une co-construction d’outils de politique publique, qui ont le potentiel de gagner le support de chaque autre acteur. Ce référentiel, une fois appliqué à la gouvernance des risques, pose les fondations pour un référentiel de la sécurité (Brunet, 2007), résultat des structures et activités de communications, elles-mêmes le canal des valeurs, normes, images et des logiques qui contribuent à la structuration de la perception individuelle et sociale du risque. Au sein du modèle de Renn, la communication devient l’élément central de chaque étape du processus d’analyse des risques. Ce processus offre un apprentissage mutuel et itératif, marquant la différence avec la simple information, unilatérale par nature, et la communication, qui permet des boucles d'enrichissement, ouvrant la possibilité aux parties prenantes (scientifiques, décideurs politiques, acteurs socio-économiques) de créer un référentiel partagé par tous les acteurs du processus. Comme illustration du fonctionnement du modèle de gouvernance des risques, nous allons analyser le cas du bisphénol A, qui fut, en 2010, le berceau d'une controverse. Cette dernière a abouti à l’adoption de la directive 2011/8/UE par la Commission Européenne (CE), restreignant l'utilisation du bisphénol A dans les biberons en plastique pour nourrissons, décision allant à l'encontre de l'avis scientifique de l'Agence Européenne pour la Sécurité Alimentaire (EFSA), qui avait établit qu'il n'y avait pas de danger pour la santé tant que la dose journalière admissible n'était pas dépassée. La controverse qui a émergé en 2010 était majoritairement une controverse publique – même si elle avait ses racines dans une controverse scientifique antérieure –, relayée par une large couverture médiatique, où les arguments émotionnels étaient plus mis en avant que les arguments scientifiques. Ces désaccords ont mis en évidence des divergences croissantes entre les opinions scientifiques et l'opinion publique : en adoptant cette directive, la CE a estimé que l'opinion publique ne pouvait plus être ignorée. Était-il possible pour la CE d'ignorer les réclamations des profanes ? Aurait-elle dû faire plus confiance aux experts de l’EFSA en outrepassant ces appels ? [less ▲]

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See detailDental extractions in patients treated with intravenous bisphosphonates and risk of osteonecrosis of jaws.
GAUDIN, Elise ULiege; HERION, Francine ULiege; ROMPEN, Eric ULiege et al

Poster (2012, June)

Aim : Dental extraction remains contra-indicated in patients treated with intravenous (IV) bisphosphonates for oncology reasons because of the high risk of bisphosphonate-related osteonecrosis of the jaw ... [more ▼]

Aim : Dental extraction remains contra-indicated in patients treated with intravenous (IV) bisphosphonates for oncology reasons because of the high risk of bisphosphonate-related osteonecrosis of the jaw (BRONJ). The objective of the present abstract was to present a preventive tooth extraction protocol in patients treated with IV bisphosphonates based on the surgical removal of the alveolar process. The second objective was to identify potential risk factors to develop BRONJ. Material and Methods : 17 patients treated with IV bisphosphonate and needing at least a tooth extraction, were included. A standardized extraction protocol was followed, including alveolectomy of at least 50% of the alveolar process, pre and post-operative antibiotherapy was administrated. The patients were followed for a mean period of 29 months (min:3 -max:62). Results : In all, 17 patients and 22 extraction sites mandible (15), maxilla (7) were involved in the study. No signs of inflamed tissue or necrotic exposed bone in any patient were observed during the follow-up period and the level of comfort for the patient was improved in all cases. Nevertheless, when a careful screening of the healing area was made using a probe, in 4 out of 22 (18,2%) sites, a remaining bone contact was found and appeared to be related location (mandible), duration of biphosphonate treatment and to concomitant. Conclusion : The present cases series suggests that the described extraction protocol in IV biphosphonate patients allowed 100% bone healing and complete soft tissues healing in 81.8% of the extraction sites.The sites that have not fully recovered seemed to be related to different factors. [less ▲]

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See detailMineralogical and Chemical Characterizations of Natural Clays from NW Cameroon
Mache, Jacques Richard ULiege; Fagel, Nathalie ULiege; Njoya, André

Poster (2012, June)

In this study physical and chemical characterization of seven clay samples from Cameroon is reported. The various analysis show that the main clay mineral in all samples is smectite with a small amount of ... [more ▼]

In this study physical and chemical characterization of seven clay samples from Cameroon is reported. The various analysis show that the main clay mineral in all samples is smectite with a small amount of kaolinite; some non clay minerals as cristobalite, K-feldspars, plagioclase, ilmenite and quartz are also present. The Cation Exchange Capacities range from 24.2 to 62.0 meq/100g. The specific surface area range from 58 to 123 m2/g. The chemical composition of the clays is dominated by SiO2, Al2O3 and Fe2O3 whereas MgO and Na2O are present only in small quantities. [less ▲]

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See detailPatient morbidity after socket preservation using a connective tissue graft versus a bilayer collagen matrix: Preliminary results of a comparative Randomized Control Trial.
FERNANDEZ AYORA, Alberto ULiege; VANHOUTTE, Vanessa ULiege; LECLOUX, Geoffrey ULiege et al

Poster (2012, June)

INTRODUCTION AND AIM: The present randomized controlled trial compared socket preservation procedures using bovine hydroxyapatite (Bio-Oss®, Geistlich, Switzerland) covered with a connective tissue graft ... [more ▼]

INTRODUCTION AND AIM: The present randomized controlled trial compared socket preservation procedures using bovine hydroxyapatite (Bio-Oss®, Geistlich, Switzerland) covered with a connective tissue graft (CT) versus a bilayer collagen membrane (CM) (Mucograft®, Geistlich, Switzerland), placed from buccal to palatal in split-thickness pouches in order to seal the extraction site and to potentially thicken the buccal mucosa. The aim of this present abstract was to evaluate post-operative complications and patient-centered outcomes of the two independent surgical protocols. METHODS: This randomized controlled trial included 26 patients (16 female, 10 male) aged from 20 to 69 years (mean: 42.6, SD: 12). 26 teeth were extracted atraumatically and the 2 distinct surgical protocols were applied randomly. Patients filled out a VAS form 1 week after the surgery to evaluate their level of discomfort and post-operative pain. Drug intakes as well as complications were also recorded. RESULTS: No statistical significant differences were found between the 2 groups in terms of post-operative complications (bleeding) and post-operative pain. The consumption of painkillers after the surgery decreased over time but was similar in the two groups. CONCLUSION: According to the preliminary results of the present RCT, none of both protocols seemed to induce significant post-operative pain and discomfort. There was no difference between the two procedures regarding post-operative complications and patient centered outcomes. [less ▲]

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See detailEarly Implant placement in socket preservations: 12-month results of a prospective case series.
DETHIER, Frédéric ULiege; LECLOUX, Geoffrey ULiege; ROMPEN, Eric ULiege et al

Poster (2012, June)

Objectives: The aim of this study was to evaluate the clinical and histological outcomes of implant placed after socket preservation procedures in the esthetic zone. Implant survival rate and biological ... [more ▼]

Objectives: The aim of this study was to evaluate the clinical and histological outcomes of implant placed after socket preservation procedures in the esthetic zone. Implant survival rate and biological complications were recorded during a follow-up period of 1 year. Materials and methods: A series of 12 patients needing the replacement of a single tooth in the esthetic zone were included in the present study. The hopeless teeth were extracted atraumatically, the sockets were filled with bovine hydroxyapatite (0.25-1mm particles) and the sites were covered with a saddled connective tissue graft. After a healing period of 3 months, biopsies were retrieved in the socket preservation sites and implants were placed. The patients were followed for a period of 1 year. Results: The etiologies of tooth loss were : endodontic (9), caries (1), periodontal (1), and traumatic (1). A total of 12 implants (10 incisors, 2 premolars) were placed on 12 patients (6 men / 6 women) aged from 24 to 61 (42,3 – 11,91). All implant reached an adequate primary stability (> 20N/cm2). 7 out of 12 implants were immediately restored with a temporary crown, and the définitive restorations were successfully placed in all cases. After a follow-up period of 1 year, all implants fulfilled strict success criteria for dental implants with regard to osteointegration, including the absence of peri-implant radiolucency, implant mobility, suppuration, and pain and no further complications occurred. Out of 12 sites only 7 biopsies were retrieved for histological analyses. New bone was found in the apical third of the socket in every specimen, in the mid-third in only 3 specimen and no bone was found in any of the spécimen in the more cervical third of the socket. Conclusion: The results from this prospective case series suggest that early implant placement in extraction socket site seems to display good clinical outcomes despite the incomplete bone regeneration in the socket at 3 months. [less ▲]

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See detailThe neural correlates of recollection and familiarity during aging
Angel, Lucie; Bastin, Christine ULiege; Genon, Sarah ULiege et al

Poster (2012, June)

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See detailDescription of a ‘soft tissue friendly’ protocol for immediate loading in the esthetic zone: 1 year result of 13 consecutive patients
COMPEYRON, Yoann ULiege; LECLOUX, Geoffrey ULiege; ROMPEN, Eric ULiege et al

Poster (2012, June)

Objectives: Repeted abutment connection and disconnections compromize the connective and epithelial sealing around implant components, leading to peri-implant bone remodeling (Abrahamsson, 1997). Placing ... [more ▼]

Objectives: Repeted abutment connection and disconnections compromize the connective and epithelial sealing around implant components, leading to peri-implant bone remodeling (Abrahamsson, 1997). Placing the definitive abutment at the time of implant placement might be a treatment option to minimize this biological rupture. The aim of this study was to evaluate the effectiveness of pre-shaped zirconium abutments for immediate implant loading in the anterior maxilla. Materials and methods: A total of 13 patients needing the placement of one or several implant in the esthetic zone were treated with Regular Crossfit Bone Level (Straumann) implants. Implants were placed and immediately restored with a pre-shaped zirconium abutments and provisional resin crowns if the primary stability reached at least 20 N/cm2. Implant survival rate, biological and prosthodontic complications as well as peri-implant tissue status (papilla index) were recorded during a follow-up period of 1 year. Results: A total of 14 implants (8 incisors, 1 canines, 5 premolars) placed in 11 patients (4 men / 7 women) aged from 22 to 60 years old (48,5 +/- 11,5) could be assessed for the entire follow-up period. 2 patients dropped out. All implants reached an adequate primary stability (> 20N/cm2) to perform an immediate implant restoration. Straight pre-shaped Zirconium abutment was used in each case. Six implants were placed in socket preservation sites and five implants were placed simultaneously with a GBR. After a follow-up period of 1 year, all implants fulfilled survival criteria for dental implants with regard to osteointegration, including the absence of peri-implant radiolucency, implant mobility, suppuration, and pain. One abutment screw loosening was observed during the provisional period and no abutment fracture occurred. Definitive full ceramic-cemented restorations were successfully performed on each implant after a mean period of 6 months and no further complications occurred. In 11 out of 14 implants, an improvement of the papilla index was observed from baseline to 1 year follow-up whereas 3 implants showed a decrease of this index. Conclusion: The results from this prospective case series suggest that immediately restored implants using a final pre-shaped zirconium abutment in esthetic zone display favorable implant and prosthodontic outcomes and seems to allow an adequate papilla index improvement in most of the cases. [less ▲]

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See detailRetrieval of methanol (CH3OH) above the high-altitude Jungfraujoch station (46.5ºN): preliminary total column time series, long-term trend and seasonal modulation
Mahieu, Emmanuel ULiege; Bader, Whitney ULiege; Bovy, Benoît ULiege et al

Poster (2012, June)

Methanol (CH3OH) is a key organic compound in the Earth’s troposphere, with reported concentrations of the order of a few ppbv. It is indeed the second most abundant organic atmospheric compound after ... [more ▼]

Methanol (CH3OH) is a key organic compound in the Earth’s troposphere, with reported concentrations of the order of a few ppbv. It is indeed the second most abundant organic atmospheric compound after methane. Its lifetime is estimated to a few days. Natural sources of CH3OH include plant growth, oceans, decomposition of plant matter, oxidation of methane,… They are complemented by anthropogenic (from vehicles, industry) and biomass burning emissions. Oxidation by the hydroxyl radical is the main sink, leading to the formation of carbon monoxide (CO) and formaldehyde (H2CO). The first reported retrievals of methanol used a microwindow extending from 992 to 999 cm-1 or from 1029 to 1037 cm-1. In both cases, lines of the strong ν8 band of CH3OH were adjusted, accounting for interferences by several isotopologues of ozone and by water vapor. In this contribution, we present first retrievals of CH3OH from observations recorded at the high-altitude station of the Jungfraujoch (46.5°N, 8°E, 3580 m asl), with a Bruker 120HR spectrometer, in the framework of the Network for the Detection of Atmospheric Composition Change (NDACC, visit http://www.ndacc.org). A strategy maximizing the information content and combining the 992-999 and 1029-1037 cm-1 domains has been set up and used. A preliminary long-term CH3OH total column time series derived from the Jungfraujoch observational database allows us to investigate the seasonal variation and long-term trend of this species at northern mid-latitudes. [less ▲]

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See detailRadiative rates for forbidden transitions in doubly-ionized Fe-peak elements
Fivet, V.; Quinet, Pascal ULiege; Bautista, M.A.

Poster (2012, June)

Detailed reference viewed: 4 (1 ULiège)