References of "Poster"
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See detailMelanopsin retinal ganglion cells in LHON patients: an fMRI study of brain activations under monochromatic light stimulation
Evangelisti, Stefania; Testa, Claudia; La Morgia, Chiara et al

Poster (2018, June)

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See detailOpal-like photoanodes with photonic effects in macroporous perovskite solar cells
Dewalque, Jennifer ULiege; Baron, Damien ULiege; Spronck, Gilles ULiege et al

Poster (2018, February 28)

Organic-inorganic metal halide perovskite compounds, used either in mesoscopic or planar solar cells architectures, have allowed preparing highly efficient solid-state devices (>20%). In mesoscopic solar ... [more ▼]

Organic-inorganic metal halide perovskite compounds, used either in mesoscopic or planar solar cells architectures, have allowed preparing highly efficient solid-state devices (>20%). In mesoscopic solar cells, a mesoporous TiO2 scaffold is incorporated into the cells. However, because of the small pore size compared to the wavelengths of visible light, the scaffold barely scatters light. In this work, we propose to design periodically structured TiO2 scaffold from colloidal crystal templating fabrication method. Polystyrene beads with diameters comparable to visible light wavelengths are used as structuring agent. The resulting opal-like photonic structure will strongly interact with light and increase light harvesting. The photoanode microstructure is characterized by scanning electron microscopy (SEM) and X-Ray diffraction (XRD). In parallel, light interaction is modeled in order to find the best compromise in terms of photonic architecture. Sunlight absorption by the solar cells is presented and discussed with dependency of pore sizes and number of opal-like layers. [less ▲]

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See detailWhat is the landscape of artisanal cheese production in Belgium?
Gerard, Amaury ULiege; Daube, Georges ULiege; Sindic, Marianne ULiege

Poster (2018, February 08)

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See detailTocopherol analysis of almond oils produced in eastern Morocco
Melhaoui, R.; Fauconnier, Marie-Laure ULiege; Sindic, Marianne ULiege et al

Poster (2018, February 08)

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See detailIntegrated continuous flow photoreactors: Photooxidation of (L)-methionine with singlet oxygen
Emmanuel, Noémie ULiege; Mendoza Gallego, Carlos ULiege; Winter, Marc et al

Poster (2018, February 06)

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See detailImproving the bioinformatics analysis of HTS clonality data in virus-induced leukemia
Hahaut, Vincent ULiege; Rosewick, Nicolas; Artesi, Maria ULiege et al

Poster (2018, February 02)

Proviral integration into the host genome is one of the main hallmarks of infection by oncogenic retroviruses. This event creates a life-long signature, each infected cell being characterized by a ... [more ▼]

Proviral integration into the host genome is one of the main hallmarks of infection by oncogenic retroviruses. This event creates a life-long signature, each infected cell being characterized by a specific integration site (IS). Monitoring of the clonal architecture over time (clone: population of cells sharing an identical IS) has significantly contributed to a better understanding of HIV persistence, gene therapy vector mediated treatment and deltaretrovirus-induced leukemia. Our lab recently developed an optimized high-throughput sequencing (HTS) based clonality method. It enables the identification of proviral integration sites genome-wide while simultaneously quantifying the abundance of the corresponding clones. The method is superior to any of the previously available protocols, mainly in terms of sensitivity, cost-effectiveness and hands-on time, making it suitable for routine clinical observation of infected individuals. Using this method, we recently showed that longitudinal monitoring of the dominant leukemic clone in patients infected by Human T-cell Leukemia Virus-1 (HTLV-1) better predicts therapeutic response (Artesi et al, Leukemia, 2017). We applied the method to biological samples isolated from HTLV-1 infected patients and Bovine Leukemia Virus (BLV) infected animals (bovine and sheep). This resulted in the generation of an unprecedented volume of raw sequence data. In this study we developed a novel clonality analysis pipeline that better exploits the potential of the method, improving previously published protocols. [less ▲]

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See detailPersonal Dose Computation with the Aid of Staff Monitoring system based on 3D Depth Cameras
Abdelrahman, Mahmoud Eid Mahmoud ULiege; Vanhavere, Filip; Struelens, Lara et al

Poster (2018, February 01)

For more than 50 years, passive dosimeters have been used to assess the dose to workers occupationally exposed to ionizing radiation. Such dosimeters are designed to measure the operational quantity Hp(10 ... [more ▼]

For more than 50 years, passive dosimeters have been used to assess the dose to workers occupationally exposed to ionizing radiation. Such dosimeters are designed to measure the operational quantity Hp(10) as an estimate of the effective dose, E, which is a quantitative expression of the “radiation detriment” that cannot be measured directly. With these dosimeters, the results are mostly known only after some time, and wearing a dosimeter is often seen as a burden by some workers. Furthermore, the uncertainties associated with the present dosimeters (within a factor of 1.5 or 2 from the real value) are not negligible. In line with the current move to more real time personal dose monitoring, we are working towards an innovative approach based on computational methods to determine occupational exposures. The aim of this research is to calculate doses to workers instead of measuring them. For this, the spatial radiation field, including energy and angular distribution, needs to be known. The real movement of the persons in a given workplace can be monitored in real-time using Time-of-Flight cameras and flexible computational phantoms representing the workers anatomy can be positioned using the tracking information. Finally, all this input data should be transferred to a tool, using Monte Carlo techniques to calculate the doses to the workers. As a first step, a tool used to track a person in 3D coordinates using Microsoft® Kinect™ was developed. The tool, which is utilizing the skeleton tracking algorithm embedded in the Kinect SDK from Microsoft, is capable of correctly tracking the worker movement in real-time. A series of validation experiments were performed to test the tracking tool and the dose calculation method. An anthropomorphic phantom was positioned on a moveable table in the horizontal irradiator of the Laboratory for Nuclear Calibration (LNK) at SCK•CEN. The phantom was moved to different distances from a Cs-137 source. The position of the phantom was monitored with the Kinect™ and the coordinates recorded. The dose to the phantom was calculated using different methods: 1. Using the reference values from the calibration facility (LNK), 2. Using VISIPLAN-3D: An analytical dose assessment tool developed at SCK•CEN, 3. Using MCNPX Monte-Carlo simulations, and 4. Using InstaDose® dosimeters based on direct ion storage technology. A comparison was made between each method and results showed good agreement between the reference, the measured and the calculated dose. This experiment was repeated with different degrees of complexity of movement of the phantom. This first test proved the validity of the methodology used. [less ▲]

See detailThe knotty phenotype of the frd3 Arabidopsis mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

Poster (2018, February 01)

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See detailBLUU-Tramp: a new H/D exchange method to identify the protein unfolding landscape
Kay, Jennifer ULiege; Corazza, Alessandra; Dumoulin, Mireille ULiege et al

Poster (2018, February 01)

Detailed reference viewed: 14 (1 ULiège)
See detailUnderstanding mesothelioma chemoresistance and implication of ADAM in this process
Bellefroid, Marine ULiege

Poster (2018, February 01)

Mesothelioma is an aggressive cancer that affects mesothelial surfaces. The most recognized risk factor for mesothelioma is asbestos exposure since the fibres can reach the pleura and are not cleared. The ... [more ▼]

Mesothelioma is an aggressive cancer that affects mesothelial surfaces. The most recognized risk factor for mesothelioma is asbestos exposure since the fibres can reach the pleura and are not cleared. The cancerization might arise 30 to 40 years after exposure. The gold standard treatment is a combination of cisplatin and pemetrexed. Unfortunately, the available therapies have a limited impact and cancer cells quickly develop resistance to treatment. Adamalysins constitute a family of zinc dependant proteases. Among them, ADAM (a disintegrin and metalloprotease) display a conserved structure made of various domains playing roles in cell to cell interactions, cell to extracellular matrix interactions as well as the shedding of some membrane proteins. Numerous ADAM are implicated in processes leading to cancer development such as inflammation, invasion, angiogenesis and resistance to chemotherapy. ADAM8 has been described to be implicated in cisplatin resistance in NSCLC and bladder cancers. This project aims at unveiling the mechanisms of treatment resistance induced by ADAM8 and other proteases in mesothelioma. The final goal will be the development of therapeutic strategies based on the inhibition of ADAM proteases. In human mesothelioma samples, ADAM8 mRNA are overexpressed as compared to normal pleura samples. Therefore, we hypothesized that ADAM8 was implicated in cancer development and we started to investigate it in vitro. We transfected AB12 murine mesothelioma cells with shRNA targeting ADAM8. These ADAM8-ShRNA cells treated with cisplatin displayed a decreased survival rate as compared to control-ShRNA treated cells. This indicates that ADAM8 might be implicated in cisplatin resistance. Concurrently, we have generated 2 different mesothelioma cell lines resistant to cisplatin. The first is derived from AB12 cell line and the second derives from a cell line obtained by a primary cancerization mice model (6627). Briefly, the cells have been treated with increasing doses of cisplatin from 0,25 µM to 2 µM, a dose that would kill parental cell lines. The resistance of the treated cells was assessed by a cell proliferation array in presence of cisplatin and we confirm that these cisplatin-treated cell lines display an increased resistance to the drug. In the very next future, we will perform a RNA sequencing experiment in order to compare the cisplatin-resistant cell lines to the matching parental cells in order to determine which pathways are modified and would explain how the cells resist. In conclusion, our results show that ADAM8 might be implicated in cisplatin resistance in mesothelioma. In our future researches, we will aim at discovering the molecular mechanisms of resistance to cisplatin. We hypothesize that our results will shed light on pathways differentially expressed between the parental and the resistant cell lines. By understanding the modified pathways, we hope to be able to prevent chemotherapy-resistant cells to emerge. [less ▲]

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See detailTargeting a coagulation-EMT axis in early metastasis
Vanwynsberghe, Aline ULiege; Lambert, Justine ULiege; Francart, Marie-Emilie ULiege et al

Poster (2018, February 01)

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See detailContinuous-flow preparation of γ-butyrolactone scaffolds from renewable fumaric and itaconic acids under photosensitized conditions
Gerardy, Romaric ULiege; Winter, Marc; Horn, Clemens R. et al

Poster (2018, February)

Detailed reference viewed: 17 (2 ULiège)
See detailRole of ADAM proteases in mesothelioma chemoresistance
Bellefroid, Marine ULiege

Poster (2018, January 27)

Detailed reference viewed: 13 (1 ULiège)
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See detailPregnant women in ED : a new specific triage algorithm
JOBE, Jérôme ULiege; POISMANS, Gaëlle ULiege; GHUYSEN, Alexandre ULiege

Poster (2018, January 20)

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See detailBenchmarking the Market Timing Skills of Hedge Funds: An Adjustment from Option Greeks
Fays, Boris ULiege; Hübner, Georges ULiege; Lambert, Marie ULiege

Poster (2018, January 18)

This paper shed new lights on hedge fund industry managers who claim to time the market. We revisit the Treynor and Mazuy model to infer the nature of the gamma trading of hedge funds style and follow the ... [more ▼]

This paper shed new lights on hedge fund industry managers who claim to time the market. We revisit the Treynor and Mazuy model to infer the nature of the gamma trading of hedge funds style and follow the framework of Hübner (2016) who provide an option-based adjustment of alpha for option-like payoffs. We feature convex and concave payoffs for directional and non-directional bets. Our model has applications in performance analysis as we show that adjusting for the convex nature of trades in hedge funds, the alpha of funds with ”smart” market timing ability (long call payoff) is, on average, increased by 0.70% per month. [less ▲]

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See detailACCROSS Project: Investigating the impacts of circulation changes on stratospheric tracers
Prignon, Maxime ULiege; Chabrillat, Simon; Mahieu, Emmanuel ULiege

Poster (2018, January 12)

The ACCROSS project (Atmospheric Composition and Circulation investigated with meteorological Reanalyses, Observational datasets and models for the Study of the Stratosphere and its changes) aims to ... [more ▼]

The ACCROSS project (Atmospheric Composition and Circulation investigated with meteorological Reanalyses, Observational datasets and models for the Study of the Stratosphere and its changes) aims to improve our understanding of the circulation changes in the stratosphere during the past three decades. To achieve this objective, miscellaneous observational datasets and model simulations are used. Here are presented the main aspects and the first preliminary results of the project. In the framework of the recent studies demonstrating the influence of stratospheric circulation changes on the trend of long-lived tracers (e.g. hydrogen chlorine), we decided to investigate the impact of these circulation changes on hydrogen fluoride (HF). Fourier-Transform infrared spectroscopy data produced at the Jungfraujoch NDACC site and satellite data (HALOE and ACE) are used to evaluate the HF trends. Moreover, to support our data interpretation, chemistry-transport model BASCOE simulations are also included. These early investigations show a modulation in HF time series at Jungfraujoch occurring around 2007. At the time being, it seems that BASCOE driven by ERA-Interim reanalysis cannot capture this modulation. [less ▲]

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See detailPreliminary characterization of the stratospheric circulation using long-lived tracers with the WACCM chemistry-climate model and observations
Minganti, Daniele ULiege

Poster (2018, January 12)

The changes in stratospheric circulation are one of the major sources of uncertainty in climate projection, therefore they are a major area of research. The current work is part of the ACCROSS ... [more ▼]

The changes in stratospheric circulation are one of the major sources of uncertainty in climate projection, therefore they are a major area of research. The current work is part of the ACCROSS (Atmospheric Composition and Circulation investigated with meteorological Reanalyses, Observational datasets and models for the Study of the Stratosphere and its changes) project, which intends to improve our understanding of the circulation changes in the past years through an extensive use of observations and model simulations of selected long-lived tracers. Here we compare simulations of a state-of-the-art Chemistry Climate Model with satellite observations of HF and N2O from February 2004 to February 2013. To accomplish this task major modifications to the model chemistry scheme have been made. This early comparison shows poor agreement in the HF distribution in the middle stratosphere for all latitudes, while in the low stratosphere the agreement is better, especially in the tropics. Since good agreement is found in the N2O distribution, the residual circulation is well represented, e.g. the model reproduces well the position of the transport barriers in the SH, this suggests that the disagreement in the HF distributions is due to an incomplete chemical scheme. A comparison with chemistry-transport models using the same chemistry scheme and boundary conditions is needed to evaluate this point. [less ▲]

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See detailEtude de l’influence de la fertilisation azotée sur le développement racinaire du froment d’hiver (Triticum aestivum L.)
Eylenbosch, Damien ULiege; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2018, January)

Le poster présente les premiers résultats obtenus dans un essai sur l'effet de fertilisation azotée sur le système racinaire du froment d'hiver. La quantification des racines a été réalisée sur base d ... [more ▼]

Le poster présente les premiers résultats obtenus dans un essai sur l'effet de fertilisation azotée sur le système racinaire du froment d'hiver. La quantification des racines a été réalisée sur base d'échantillons de sol analysés, après lavage, par imagerie hyperspectrale proche infrarouge. Cette méthode d'analyse de prélèvements racinaires permet de quantifier les racines sans devoir les séparer préalablement des résidus de culture présents dans les échantillons de sol et sans devoir les peser. Pour le prélèvement racinaire réalisé au stade redressement de la culture de froment, aucun effet de la fertilisation azotée apportée au stade tallage n'a pu être mis en évidence. Une diminution de la densité racinaire sur les 90 cm de sol étudiés a par contre été clairement mesurée. [less ▲]

Detailed reference viewed: 18 (0 ULiège)
See detailDeveloping Tools for Evaluating Chromatin Preps for Porcine Functional Genomics
Tuggle, Christopher K.; Huang, Jianzhen; Beiki, Hamid et al

Poster (2018)

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See detailSensitivity of the Antarctic surface mass balance to oceanic perturbations
Kittel, Christoph ULiege; Amory, Charles ULiege; Agosta, Cécile ULiege et al

Poster (2017, December 15)

Regional climate models (RCMs) are suitable numerical tools to study the surface mass balance (SMB) of the wide polar ice sheets due to their high spatial resolution and polar-adapted physics. Nonetheless ... [more ▼]

Regional climate models (RCMs) are suitable numerical tools to study the surface mass balance (SMB) of the wide polar ice sheets due to their high spatial resolution and polar-adapted physics. Nonetheless, RCMs are driven at their boundaries and over the ocean by reanalysis or global climate model (GCM) products and are thus influenced by potential biases in these large-scale fields. These biases can be significant for both the atmosphere and the sea surface conditions (i.e. sea ice concentration and sea surface temperature). With the RCM MAR, a set of sensitivity experiments has been realized to assess the direct response of the SMB of the Antarctic ice sheet to oceanic perturbations. MAR is forced by ERA-Interim and anomalies based on mean GCM biases are introduced in sea surface conditions. Results show significant increases (decreases) of liquid and solid precipitation due to biases related to warm (cold) oceans. As precipitation is mainly caused by low-pressure systems that intrude into the continent and do not penetrate far inland, coastal areas are more sensitive than inland regions. Furthermore, warm ocean representative biases lead to anomalies as large as anomalies simulated by other RCMs or GCMs for the end of the 21st century. [less ▲]

Detailed reference viewed: 38 (10 ULiège)
See detailSystematic capture of MeV electron beams by MWR
Santos-Costa, Daniel; Bellotti, Amadeo; Janssen, Mike et al

Poster (2017, December 13)

Every ~ 53 days since August 2016, Juno swings by Jupiter and as the spacecraft spins along a polar orbit, measurements of Jupiter's microwave radiation are carried out at high data rates for several ... [more ▼]

Every ~ 53 days since August 2016, Juno swings by Jupiter and as the spacecraft spins along a polar orbit, measurements of Jupiter's microwave radiation are carried out at high data rates for several hours (~ 9 hours) with the Juno Microwave Radiometer (MWR). Within ~ 6 planetary radii (Rj) and from inside/outside the magnetospheric region, the thermal and synchrotron emissions are measured at high temporal and spatial resolutions. In this paper, we present a synthesis of the spatial distributions of the microwave radiation and discuss the similarities and differences observed at six wavelengths (1.3-50 cm). In addition to the thermal emission and synchrotron radiation from Jupiter's electron belt, unexpected signatures in MWR are either systematically or sporadically reported during perijove 1 (PJ1) and PJ3-PJ6. The preliminary results of a multi-instrument analysis of radio (MWR), extreme and far-ultraviolet auroral emissions (Juno UVS), field (Juno magnetometer), keV electrons (JEDI), and background radiation signatures in Juno's ASC and SRU instruments suggest that some of these signatures are consistent with the capture by MWR of synchrotron emission radiated by MeV electron beams, which may be associated with auroral activity. We subsequently describe in detail our data analysis and effort to model the synchrotron radiation from MeV electron beams to support our findings. [less ▲]

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See detailJuno-UVS observation of the Io footprint: Influence of Io’s local environment and passage into eclipse on the strength of the interaction
Hue, Vincent; Gladstone, Randy; Greathouse, Thomas K et al

Poster (2017, December 13)

The Juno mission offers an unprecedented opportunity to study Jupiter, from its internal structure to its magnetospheric environment. Juno-UVS is a UV spectrograph with a bandpass of 70<λ<205 nm, built to ... [more ▼]

The Juno mission offers an unprecedented opportunity to study Jupiter, from its internal structure to its magnetospheric environment. Juno-UVS is a UV spectrograph with a bandpass of 70<λ<205 nm, built to characterize Jupiter’s UV emissions and provide remote sensing capacities for the onboard fields and particle instruments (MAG, Waves, JADE and JEDI). Juno’s orbit allows observing Jupiter from a unique vantage point above the poles. In particular, UVS has observed the instantaneous Io footprint and extended tail as Io enters into eclipse. This observation may better constrain whether the atmosphere of Io is sustained via volcanic activity or sublimation. Among other processes, the modulation of Io’s footprint brightness correlates to the strength of the interaction between the Io plasma torus and its ionosphere, which, in turn, is likely to be affected by the atmospheric collapse. UVS observed the Io footprint during two eclipses that occurred on PJ1 and PJ3, and one additional eclipse observation is planned during PJ9 (24 Oct. 2017). We present how the electrodynamic coupling between Io and Jupiter is influenced by changes in Io’s local environment, e.g. Io’s passage in and out of eclipse and Io’s traverse of the magnetodisc plasma sheet. [less ▲]

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See detailElectron Pitch Angle Distributions Along Field Lines Connected to the Auroral Region from ~25 to ~1.2 RJ Measured by the Jovian Auroral Distributions Experiment-Electrons (JADE-E) on Juno
Allegrini, Frederic; Bagenal, Fran; Bolton, Scott J et al

Poster (2017, December 13)

The Jovian Auroral Distributions Experiment (JADE) on Juno provides critical in situ measurements of electrons and ions needed to understand the plasma distributions and processes that fill the Jovian ... [more ▼]

The Jovian Auroral Distributions Experiment (JADE) on Juno provides critical in situ measurements of electrons and ions needed to understand the plasma distributions and processes that fill the Jovian magnetosphere and ultimately produce Jupiter’s bright and dynamic aurora. JADE is an instrument suite that includes two essentially identical electron sensors (JADE-Es) and a single ion sensor (JADE-I). JADE-E measures electron energy distributions from ~0.1 to 100 keV and provides detailed electron pitch angle distributions (PAD) at ~7.5° resolution. Juno’s trajectories in the northern hemisphere have allowed JADE to sample electron energy and pitch angle distributions on field lines connected to the auroral regions from as close as ~1.2 RJ all the way to distances greater than 25 RJ. Here, we report on the evolution of these distributions. Specifically, the PADs change from mostly uniform at distances greater than ~20 RJ, to butterfly from ~18 to ~12 RJ, to field aligned or pancake, depending on the energy, closer to Jupiter. Below ~1.5 RJ, electron beams and loss cones are observed. [less ▲]

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See detailObservation and simulation of ethane (C2H6) at 23 FTIR sites
Mahieu, Emmanuel ULiege; Franco, B; Pozzer, A et al

Poster (2017, December 13)

Ethane is the most abundant non-methane hydrocarbon (NMHC) in the Earth atmosphere. Its main sources are of anthropogenic origin, with globally 62% from leakage during production and transport of natural ... [more ▼]

Ethane is the most abundant non-methane hydrocarbon (NMHC) in the Earth atmosphere. Its main sources are of anthropogenic origin, with globally 62% from leakage during production and transport of natural gas, 20% from biofuel combustion and 18% from biomass burning. In the Southern hemisphere, anthropogenic emissions are lower which makes biomass burning emissions a more significant source. The main removal process is oxidation by the hydroxyl radical (OH), leading to a mean atmospheric lifetime of 2 months. Until recently, a prolonged decrease of its abundance has been documented, at rates of -1 to -2.7%/yr, with global emissions dropping from 14 to 11 Tg/yr over 1984-2010 owing to successful measures reducing fugitive emissions from its fossil fuel sources. However, subsequent investigations have reported on an upturn in the ethane trend, characterized by a sharp rise from about 2009 onwards. The ethane increase is attributed to the oil and natural gas production boom in North America, although significant changes in OH could also be at play.In the present contribution, we report the trend of ethane at 23 ground-based Fourier Transform Infrared (FTIR) sites spanning the 80ºN to 79ºS latitude range. Over 2010-2015, a significant ethane rise of 3-5%/yr is determined for most sites in the Northern Hemisphere, while for the Southern hemisphere the rates of changes are not significant at the 2-sigma uncertainty level. Dedicated model simulations by EMAC (ECHAM5/MESSy Atmospheric Chemistry; ~1.8×1.8 degrees) implementing various emission scenarios are included in order to support data interpretation. The usual underestimation of the NMHCs emissions in the main inventories is confirmed here for RCP85 (Representative Concentration Pathway Database v8.5). Scaling them by 1.5 is needed to capture the background levels of atmospheric ethane. Moreover, additional and significant emissions (~7 Tg over 2009-2015) are needed to capture the ethane rise in the Northern hemisphere. Attributing them to the oil and gas sector and locating them in North America allows EMAC to produce adequate trends in the Northern hemisphere, but not in the Southern hemisphere, where they are overestimated. Possible causes for this difference are discussed. [less ▲]

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See detailEffet de la cuisson sur le profil en fibres de quelques légumes tropicaux
Kalala Bolokango, Gaetan ULiege; KAMBASHI, Bienvenu; Everaert, Nadia ULiege et al

Poster (2017, December 13)

Outre le monde occidental, les pays africains dont la population a souffert de malnutrition intra-utérine sont également exposés au fléau de l’obésité à l’âge adulte en raison d’effets de programmation de ... [more ▼]

Outre le monde occidental, les pays africains dont la population a souffert de malnutrition intra-utérine sont également exposés au fléau de l’obésité à l’âge adulte en raison d’effets de programmation de long terme induits par les carences dans le jeune âge. La consommation de fibres alimentaires (DF) demeure indispensable pour la modulation du microbiote intestinal afin de prévenir cette maladie et les pathologies associées. Les légumes et les fruits sont des principales sources naturelles de DF. . En Afrique tropicale humide et sub-humide, les légumes feuilles sont fortement consommés par les populations rurales, tandis que les populations urbaines modifient leur régime alimentaire en l’ « occidentalisant ». Afin de mieux comprendre comment les changements alimentaires modifient les modes de consommation des fibres alimentaires, face à la déficience d’information dans la littérature à ce sujet, il convient de caractériser le profil en fibres de légumes méconnus et l’impact de la cuisson sur celui-ci. [less ▲]

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See detailRole for PARs and thrombin-GPIbα interaction in thrombin induced procoagulant platelet formation
Bourcy, Morgane ULiege; Wang, Samuel; Pasalic, Leonardo et al

Poster (2017, December 11)

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See detailInvestigation of the shielding properties of a Bi-2212 bulk superconducting tube subjected to an inhomogeneous magnetic field at various temperatures
Hogan, Kevin ULiege; Wera, Laurent ULiege; Fagnard, Jean-François ULiege et al

Poster (2017, December 11)

Magnetic shielding with bulk superconductors is commonly studied under homogeneous magnetic fields. This configuration simplifies the analysis of the magnetic reaction of the superconductor as the applied ... [more ▼]

Magnetic shielding with bulk superconductors is commonly studied under homogeneous magnetic fields. This configuration simplifies the analysis of the magnetic reaction of the superconductor as the applied magnetic field is known precisely everywhere. In practical applications, however, the magnetic field to be screened is seldom homogeneous. Magnetic shields experience magnetic field gradients and flux lines curvatures that might lead to a deterioration of the shielding properties. In the present work, we investigate experimentally the magnetic shielding properties of a bulk superconducting Bi-2212 tube subjected to an inhomogeneous magnetic field. This study is carried out at various temperatures thanks to a bespoke variable temperature measurement system based on a cryocooler. A superconducting pancake coil, made of 1G superconducting tape (Bi-2223), generates the inhomogeneous magnetic field to be screened. The bulk superconducting tube is anchored thermally to a sample holder fixed in the vicinity of the cold head. A cryogenic Hall probe is placed at the center of the bulk Bi-2212 tube to characterize the shielding properties of the superconductor. With this system, we characterize the threshold induction, i.e. the maximal applicable magnetic field below which the shielding factor (ratio between the measured and applied field) drops below a threshold value, at various temperatures ranging from 60 K to 92 K. Then we show and discuss the effect of the sweep rate of the applied magnetic field on the threshold induction value. Our experimental results are confronted to experimental results obtained at 77 K in an axial homogeneous magnetic field. This allow us to emphasize the impact of the inhomogeneity of the applied magnetic field on the shielding properties. We finally point out the portions of the magnetic field shield experiencing the highest magnetic field. [less ▲]

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See detailSystèmes d'informations foncières et politiques publiques (2015 - 2016)
Hendrickx, Sébastien ULiege; Ruelle, Christine ULiege; Zangl, Stéphanie ULiege et al

Poster (2017, December 11)

La recherche s’intéresse à la question de l’intérêt de développer l’observation foncière en Wallonie. Par une approche documentaire, sur la base d’entretiens auprès de témoins privilégiés et par une ... [more ▼]

La recherche s’intéresse à la question de l’intérêt de développer l’observation foncière en Wallonie. Par une approche documentaire, sur la base d’entretiens auprès de témoins privilégiés et par une enquête adressée à l’ensemble des communes wallonnes, elle tente d’évaluer la prise en compte des systèmes d’informations foncières en vue de mener à bien une politique d’aménagement du territoire efficace. Le poster présente les objectifs de cette recherche et les résultats acquis lors de la subvention 2015 - 2016. [less ▲]

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See detailPolitique wallonne de la ville : (1) Programme de développement urbain / (2) Cadre stratégique / (3) Quartiers prioritaires / (4) Activités économiques
Bianchet, Bruno ULiege; Claeys, Dorian ULiege; Mercenier, Chloé ULiege et al

Poster (2017, December 11)

Posters présentant les analyses et recommandations émises en vue de la mise en place éventuelle d'une politique de la ville en Wallonie, dans le cadre d'une recherche menée au sein de la Conférence ... [more ▼]

Posters présentant les analyses et recommandations émises en vue de la mise en place éventuelle d'une politique de la ville en Wallonie, dans le cadre d'une recherche menée au sein de la Conférence Permanente du Développement Territorial (CPDT). [less ▲]

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See detailCharges d'urbanisme et principe de proportionnalité
Hendrickx, Sébastien ULiege; Defer, Valentine ULiege; Maldague, Hubert ULiege et al

Poster (2017, December 11)

La recherche concerne les obligations imposées au promoteur et plus particulièrement le concept d’ « obligations de faire liées aux permis », soit les obligations de fournir une prestation, un service ou ... [more ▼]

La recherche concerne les obligations imposées au promoteur et plus particulièrement le concept d’ « obligations de faire liées aux permis », soit les obligations de fournir une prestation, un service ou un versement d’argent que peuvent imposer les autorités compétentes dans le cadre de la délivrance d’un permis d’urbanisme ou d’urbanisation. Le poster présente les objectifs de la recherche, la méthodologie et les résultats engrangés. [less ▲]

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See detailActivités agro-économiques: Besoins et attentes des acteurs de la filière céréalière wallonne
Andre, Marie ULiege; Bruggeman, Derek; Coppens, Alain et al

Poster (2017, December 11)

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See detailActivités agro-économiques: Méthodologie établie pour répondre aux objectifs de la recherche
Bruggeman, Derek; Coppens, Alain; Courtois, Xavier et al

Poster (2017, December 11)

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See detailSynthesis and Complexation of Sterically Hindered Imidazolium-2-dithiocarboxylate Ligands
Beltran Alvarez, Tomás Francisco ULiege; Zaragoza, Guillermo; Delaude, Lionel ULiege

Poster (2017, December 07)

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See detailThe Arabidopsis frd3 mutant phenotype: a new level of complexity
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

Poster (2017, December 07)

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See detailCassini UVIS Auroral Observations in 2016 and 2017
Pryor, Wayne R.; Esposito, Larry W.; Jouchoux, Alain et al

Poster (2017, December 06)

In 2016 and 2017, the Cassini Saturn orbiter executed a final series of high-inclination, low-periapsis orbits ideal for studies of Saturn's polar regions. The Cassini Ultraviolet Imaging Spectrograph ... [more ▼]

In 2016 and 2017, the Cassini Saturn orbiter executed a final series of high-inclination, low-periapsis orbits ideal for studies of Saturn's polar regions. The Cassini Ultraviolet Imaging Spectrograph (UVIS) obtained an extensive set of auroral images, some at the highest spatial resolution obtained during Cassini's long orbital mission (2004-2017). In some cases, two or three spacecraft slews at right angles to the long slit of the spectrograph were required to cover the entire auroral region to form auroral images. We will present selected images from this set showing narrow arcs of emission, more diffuse auroral emissions, multiple auroral arcs in a single image, discrete spots of emission, small scale vortices, large-scale spiral forms, and parallel linear features that appear to cross in places like twisted wires. Some shorter features are transverse to the main auroral arcs, like barbs on a wire. UVIS observations were in some cases simultaneous with auroral observations from the Cassini Imaging Science Subsystem (ISS) the Cassini Visual and Infrared Mapping Spectrometer (VIMS), and the Hubble Space Telescope Space Telescope Imaging Spectrograph (STIS) that will also be presented. [less ▲]

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See detailIntérêt du Western blot 2D dans les réactions croisées avec les insectes
Courtois, Justine ULiege; Tollenaere, Stéphanie; Quinting, Birgit et al

Poster (2017, December 01)

Introduction L’entomophagie est une alternative alimentaire qui devient, depuis quelques années, une pratique de plus en plus courante dans nos pays. Le but de notre étude est donc d’identifier les ... [more ▼]

Introduction L’entomophagie est une alternative alimentaire qui devient, depuis quelques années, une pratique de plus en plus courante dans nos pays. Le but de notre étude est donc d’identifier les potentielles réactions croisées entre les allergènes des crustacés, des acariens et des grillons (Gryllodes sigillatus). Matériel & Méthodes Nous avons sélectionné 12 patients présentant une allergie aux acariens et/ou aux crustacés sur base de leur positivité en IgE spécifiques (IgEs) dirigés contre deux tropomyosines, Der p 10 (acariens) et contre Pen a 1 (crevettes) dosées par ImmunoCAP250 (ThermoFisher Scientific). Ensuite, nous avons réalisé une extraction protéique totale à partir de grillons séchés dans le but de séparer les protéines selon leurs points isoélectriques et leurs poids moléculaires. Finalement, nous avons réalisé un Western blot (WB) 1D suivi de WB 2D afin de déterminer les allergènes moléculaires responsables d’un profil de sensibilisation pour chaque sérum de patients testés contre cet extrait protéique. Résultats & Discussion Le WB 1D a confirmé la réactivité des IgEs contre une protéine située aux environs de 37 kDa qui pourrait être soit la tropomyosine soit l’arginine kinase (AK) du grillon. Le WB 2D a également confirmé une sensibilisation contre une protéine située aux alentours de 37 kDa, pH 3-4, probablement la tropomyosine et/ou contre une protéine localisée aux environs de 37 kDa, pH 6-7, probablement l’AK. De plus, une zone a également été mise en évidence aux environs de 17,5 kDa, pH 4 qui pourrait être la troponine C, une autre protéine allergénique décrite. Conclusion Nos résultats préliminaires ont montré qu’il y avait, bel et bien, une réaction croisée entre les crustacés/acariens et les grillons. Les protéines en cause sont la tromomyosine et/ou l’AK ainsi que la troponine C. Ces hypothèses seront confirmées par spectrométrie de masse (LC-MS/MS). [less ▲]

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See detailLe moment d’apparition de la fatigue mentale varie-t-il en fonction de l’âge ? Détermination du Time-on-Task effect chez des jeunes, des middle-age, et des âgés.
Gilsoul, Jessica ULiege; Collette, Fabienne ULiege

Poster (2017, December 01)

Introduction. La fatigue mentale peut survenir chez des personnes jeunes après un certain temps de tâche (Time-on-Task effect). Wang et al. (2016) ont mis en évidence une augmentation des temps de ... [more ▼]

Introduction. La fatigue mentale peut survenir chez des personnes jeunes après un certain temps de tâche (Time-on-Task effect). Wang et al. (2016) ont mis en évidence une augmentation des temps de réaction de jeunes personnes entre les 40 premières et les 40 dernières minutes d’une tâche Stroop de 160min. Notre étude a pour but de répliquer les résultats de Wang et al. (2016) et de tester pour la première fois le Time-on-Task effect chez des middle-age et des âgés. Méthode. Vingt-et-un jeunes (MÂge=21,29), 16 middle-age (MÂge = 49,94), et 17 âgés (MÂge = 65,06) ont réalisé une tâche Stroop durant 160min. Celle-ci comprenait des items facilitateurs (FA), interférents (I), et neutres (NE), ces derniers apparaissant systématiquement un item sur deux. Les 160min. ont été divisées en huit blocs de 20min. avec élimination du Bloc 0 (Bloc d’habituation). Des ANOVAs 3(Groupe : Jeune, Middle-age, Âgé) X 7(Bloc : 1,…,7) à mesures répétées ont été réalisées sur les moyennes et les coefficients de variation (CV) des temps de réaction (TR) des trois types d’items. Résultats. Moyenne. Les jeunes montrent une augmentation de leur TR entre le 1er et le 5ème Bloc (p=,03) sur les items NE alors que cette augmentation survient lors du Bloc 4 pour les middle-age (p=,006) et les âgés (p=,04). CV. Les jeunes montrent une augmentation de leur CV entre le Bloc 1 et le bloc 7 (p=,002) sur les items NE alors que celle-ci survient lors du Bloc 6 pour les middle-age (p=,02). En revanche, le CV des âgés ne varie pas en fonction des blocs. Discussion. La fatigue mentale mesurée grâce au Time-on-Task dans le vieillissement semble se manifester par un ralentissement général du traitement de l’information à mesure de l’avancée sur la tâche : les middle-age et les âgés étant plus précocement fatigués que les sujets jeunes. En revanche, alors que les jeunes et les middle-age semblent affectés au niveau de leur CV, le vieillissement plus avancé semble préserver la variabilité intra-individuelle du Time-on-Task. De façon intéressante, alors que les items FA et I semblent moins sensibles à la fatigue mentale, les trois groupes ont montré un ralentissement significatif de leur vitesse sur les items NE à mesure du Time-on-Task. Etant donné la prévisibilité des NE dans notre paradigme, nous faisons l’hypothèse que la fatigue mentale survient plus facilement lorsque l’individu peut systématiquement anticiper l’information à traiter, le menant à un désengagement de la tâche. [less ▲]

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See detailDissociation entre recollection objective et subjective dans le vieillissement normal : une approche par analyses multiniveaux
Folville, Adrien ULiege; D'Argembeau, Arnaud ULiege; Bastin, Christine ULiege

Poster (2017, December 01)

Bien que le vieillissement normal soit associé à un déclin en recollection lorsque ce processus est mesuré de façon objective (mémoire de source ou rappel libre), l’expérience subjective de recollection ... [more ▼]

Bien que le vieillissement normal soit associé à un déclin en recollection lorsque ce processus est mesuré de façon objective (mémoire de source ou rappel libre), l’expérience subjective de recollection (vivacité du souvenir) demeure stable au cours du vieillissement, suggérant l’existence d’une dissociation entre ces deux processus (Mcdonough et al. 2014). A ce jour, les recherches ont toujours comparé ces deux aspects de la recollection séparément en mesurant les scores moyens de chaque participant, si bien que la relation entre mesures objective et subjective de recollection avec une approche essai-par-essai reste inconnue. Afin d’examiner cette question, nous avons recruté 34 participants jeunes et 34 participants âgés. Ils ont pris part à une tâche de recollection indicée au cours de laquelle ils mémorisaient des images complexes associées des titres descriptifs, chaque image étant présentée à gauche ou à droite de l’écran et avant ou après une pause. A la récupération, pour chaque essai, les participants devaient répondre à une question de mémoire de source spatiale ou temporelle et émettre un jugement de vivacité concernant leur souvenir de l’image associé au titre. Ensuite, les participants étaient invités à rappeler oralement autant d’éléments que possible concernant l’image. Des analyses multiniveaux ont été utilisées pour évaluer la relation entre les mesures de recollection subjective (vivacité) et objective (rappel libre) à travers les essais dans les deux groupes. Les résultats ont mis en évidence une différence significative entre les groupes en mémoire de source. Les participants âgés ont émis des jugements de vivacité plus élevés que les participants jeunes bien que leurs rappels libres soient moins détaillés, mettant ainsi en évidence une dissociation entre ces mesures. Les analyses multiniveaux ont révélé que la quantité de détail rappelé était un prédicteur significatif du jugement de vivacité dans les deux groupes, bien que cette relation soit plus importante chez les sujets jeunes par rapport aux âgés. Nos mesures de recollection objective suggèrent que le vieillissement normal diminue notre capacité à récupérer des informations contextuelles et des détails visuels associés à des souvenirs complexes. Les analyses multiniveaux suggèrent que les participants âgés ajusteraient moins bien leurs jugements subjectifs de vivacité vis-à-vis de la quantité d’information qu’ils récupèrent en mémoire comparé aux jeunes. Nous émettons l’hypothèse que des changements métacognitifs liés à l’âge ou qu’une utilisation d’informations différentes pour émettre un jugement de vivacité puisse expliquer cette faible correspondance entre recollection objective et subjective dans le vieillissement normal. [less ▲]

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See detailEvaluation of ground reaction forces based on kinematic data
Van Hulle, Romain ULiege; Schwartz, Cédric ULiege; Bruls, Olivier ULiege

Poster (2017, December 01)

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See detailTaxonomic characterisation of bacteria communities from water of diversified aquaponic systems
Eck, Mathilde ULiege; Sare, Abdoul Razack ULiege; Massart, Sébastien ULiege et al

Poster (2017, December)

In 2030, the world’s population should reach 8.3 billion people. It is thus necessary to find intensive yet sustainable food production methods to feed this growing population and aquaponics could ... [more ▼]

In 2030, the world’s population should reach 8.3 billion people. It is thus necessary to find intensive yet sustainable food production methods to feed this growing population and aquaponics could contribute to it.. Aquaponics is defined as a combination of hydroponic and aquaculture techniques and seems to be a promising technology to meet this resilience. It functions with plants, fish and microorganisms which play a key role in nitrification and mineralisation of fish wastes into nutrients absorbable by plants. Herein we aim at characterising the bacteria present in diversified systems to better understand the composition and role of their communities in aquaponics. To this end, nine diversified aquaponic systems were sampled. The DNA from each bacteria community was extracted and sequenced with Illumina MiSeq technology by targeting the V1-V3 16S rDNA region. The sequences were then analysed with the QIIME bioinformatic software. Results show that Proteobacteria and Bacteroidetes are the dominant phyla for all the aquaponic systems. Depending on each system, different proportions of other phyla are also present among the bacterial community. The genera which compose all the identified phyla are more diverse and an important proportion of them are usually found in soils and rhizosphere. One of the roles that could be linked to these genera is the breaking down of complex organic compounds which could be related to the mineralisation phenomenon observed in aquaponic systems. Further studies should be undertaken to identify the exact species present in aquaponic systems and to understand their specific functions. [less ▲]

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See detailContinuous-flow preparation of γ-butyrolactone scaffolds from renewable fumaric and itaconic acids under photosensitized conditions
Gerardy, Romaric ULiege; Winter, Marc; Horn, Clemens R. et al

Poster (2017, December)

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See detailElemental composition of soils and sediments by using portable X-ray fluorescence (pXRF) spectroscopy: a preliminary study
Alberti, Roberto; Falsone, Gloria; Gironda, M. et al

Poster (2017, December)

Portable X-ray fluorescence spectrometry (pXRF) provides rapid and low-cost analysis in laboratory and field conditions for a wide range of environmental applications. Its successful application in soils ... [more ▼]

Portable X-ray fluorescence spectrometry (pXRF) provides rapid and low-cost analysis in laboratory and field conditions for a wide range of environmental applications. Its successful application in soils requires a good instrument calibration and the possibility to implement custom calibrations and methods using known samples as well as reference materials. In the present study two set of samples, one from pedogenized sediments of a basin in Marradi (Italy; n=13) and the other from agricultural land in Meuse valley (Belgium; n=10), were analyzed by using a portable X-ray fluorescence (pXRF TRACER 5i made by Bruker) to detect and quantify the element species (Al, Si, Ca, Fe, Mg, K, Mn, P, S, Ba, Cd, Co, Cr, Cu, Mo, Pb, Sb, Sn, Sr and Zn). Element concentrations results were compared with analytical results from aqua regia extractions (AQ) followed by ICP-OES analysis. The aim of this work was to verify and find the best instrument setup for the specific matrix, elements and their concentration range with the purpose of using the pXRF technique directly insitu for the screening of sites potentially polluted. A standard factory calibration for soil applications was used for samples from Meuse valley, while a custom calibration for P, S, Ca and Fe quantification with the coefficient correction method was developed and used for pedogenized sediments from Marradi, as well as for Cu and Cr quantification in both sites. Comparing portable XRF and AQ results, elements could be grouped in three classes: 1) elements where the content determined by both methods is not correlated (p>0.05; Al, Si, and K); 2) elements strongly correlated (0.84<r<0.99, p<0.001; P, S, Mn, Fe, Ca, Cu, Cr, Zn and Pb); 3) elements with a weaker correlation (0.67<r<0.78, p<0.01; Mg and Sr). A further class of elements (Co, Mo, Cd, Sn, Sb, and Ba) was not evaluable because their concentration was below the detection limit of the pXRF. The measured concentrations and the relationship between pXRF and AQ were obviously affected by the elements form in the soils. The value of macroelements commonly related to the silicate phase measured by portable XRF is higher than that determined by AQ, which does not allow the destruction of Si-O and Al-O bounds (explaining the uncorrelation in the class 1 elements). The elements related to the organic matter (P and S, r2>0.60), carbonates (Ca, r2>0.55) and oxy(hydr)oxides (Fe and Mn) have instead comparable concentrations between pXRF and AQ. Preliminary results showed that portable XRF can quickly provide data for some elucidations of important pedogenic processes involving many elements (class 2) in the studied soils. Alternative methods offer results after expensive and time-consuming analytical procedures. Moreover for environmental and human health purposes, the pXRF should be a valid tool for a rapid screening of heavy metals, such as Cu, Cr, Zn and Pb. A larger set of soil samples and further custom calibration will be tested. [less ▲]

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See detailGC-MS Orbitrap and GC×GC-(HR)TOFMS in colorectal cancer metabolomics
Di Giovanni, Nicolas ULiege; Cojocariu, C; Silcock, P et al

Poster (2017, December)

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See detailAntifungal effects of volatile organic compounds emitted during infection of barley roots by fungal pathogens
De Clerck, Caroline ULiege; Fiers, Marie; Jallais, Lucie et al

Poster (2017, December)

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See detailShould we all eat insects ?
Courtois, Justine ULiege; Goddé, Marie; Tollenaere, Stéphanie et al

Poster (2017, November 25)

Introduction Entomophagy is an alternative food that has become more common in our countries during recent years. The aim of the study was to identify the potential cross-reactivity between the allergens ... [more ▼]

Introduction Entomophagy is an alternative food that has become more common in our countries during recent years. The aim of the study was to identify the potential cross-reactivity between the allergens of shrimps, House Dust Mites (HDM) and crickets. Material & Method We selected 3 patients (aged 7–18–27 y.o.) on the basis of their positive sIgE results against Der p 10 (from 16.9 to >100 KUA/L) and against Pen a 1 (from 14.3 to >100 KUA/L), two tropomyosins. Each patient had a diagnosis of both HDM allergy and food allergy to shrimps. We performed a total Gryllodes sigillatus protein extraction in order to separate the proteins on the basis of their isoelectric point and their molecular weight. Afterwards, we performed 1D and 2D Western blot (WB) to determine the molecular allergen sensitization profile of each patient serum to the extract. Results & Discussion The 1D WB confirmed the sIgE reactivity to a protein around 37 kDa that could be the Gryllodes’ tropomyosin. The 2D WB confirmed for the 3 patients’ sera a tropomyosin sensitization (around 37 kDa, pH 5-7). Furthermore, it showed for 1 out of 3 patients a sensitization to a protein around 17 kDa, pH 9 that could be troponin, another described allergenic protein. Conclusion Our preliminary results showed IgE cross-reactivities with the Gryllodes’ tropomyosin in 3 shrimp and HDM allergic patients with positive sIgE to Pen a 1 and Der p 10. One patient presented a sensitization to the Gryllodes’ troponin, but the identification of this protein should be confirmed by LC-MS/MS. [less ▲]

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See detailEco-extraction of aniseed oil: Use of green solvent as alternative solvent to n-hexane
Bettaieb ép Rebey, Ines ULiege; Bourgou, Soumaya; Detry, Pauline ULiege et al

Poster (2017, November 25)

Pimpinella anisum L. (anise) seeds have been widely used as a culinary ingredient as well as traditional remedies for the treatment of different disorders in the folk medicine systems of different ... [more ▼]

Pimpinella anisum L. (anise) seeds have been widely used as a culinary ingredient as well as traditional remedies for the treatment of different disorders in the folk medicine systems of different civilizations. Its essential oil show many properties and is already well studied and used. The lipid fraction of the seeds has also already been investigated. Petroselinic acid was the most prevalent fatty acid in anis oil seeds. This one also presents several properties. Nowadays, fixed oils are extracted with the help of petrosolvents. This raise issues regarding environment and security, but also public health. Regulations are more and more strict and alternative more and more searched. In general, those ones consist in the use of substitution solvents. Thus, the purpose of this work was designed to evaluate the performances of a green solvent compared to n-hexane in aniseed oil extraction. [less ▲]

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See detailHow immunoblotting and mass spectrometry can help to diagnose mustard allergy
Courtois, Justine ULiege; BERTHOLET, Catherine ULiege; Cavalier, Etienne ULiege et al

Poster (2017, November 24)

Introduction We describe a clinical case study of severe allergy to a variety of mustard, Sinapis alba, in an adult patient without any previous food nor respiratory allergy history. Objectives The ... [more ▼]

Introduction We describe a clinical case study of severe allergy to a variety of mustard, Sinapis alba, in an adult patient without any previous food nor respiratory allergy history. Objectives The diagnosis of allergy to mustard is based on anamnesis, skin prick test and specific IgE (sIgE) measurement to total mustard extract. Actually, the in vitro diagnostic tools cannot help the physician to define the precise mustard allergens involved in the allergic reaction and are unable to support evaluation of potential cross-reactions. Indeed, no molecular allergen component is commercially available for mustard. We aimed to adapt a 2D immunoblot method to mustard. Afterwards, mass spectrometry (LC-MS/MS) was used to identify precisely the allergens bound to sIgE. Methods We analyzed the serum of a 37 y.o. man presenting a grade 2 reaction (facial quincke edema with respiratory distress) after eating food containing the mustard species Sinapis alba. He had positive sIgE results for mustard extract (0.62 KUA/L) and a positive realistic SPT to foods containing mustard. We extracted total proteins of Sinapis alba seed. The different proteins were separated based on their isoelectric point and their molecular weight. The patient serum was analyzed by 2D Western blot in order to evaluate its sIgE reactivity against the different protein spots. Finally, the protein spots recognized by the patient sIgE were precisely identified by LC-MS/MS. Results The patient sIgE sensitization profile showed three specific protein spots. The first protein spot was observed at 18 kDa and pH 5 to 6. A second protein spot was localized around 14 kDa and pH 5. Finally, the third protein spot was situated around 15 kDa and pH 7. The LC-MS/MS analysis of these 3 spots pointed out 2 allergens already described in mustard allergy: sin a 1 (2S-albumin) and sin a 2 (11S-globulin). Conclusion In this study, a 2D immunoblot provided a specific sensitization profile for a patient presenting a grade 2 allergy to mustard with low sIgE to total mustard extract and without any other history of allergy. The protein spots recognized by the sIgE concerned two main allergens identified by LC-MS/MS as sin a 1 and sin a 2. Those allergens are classified in the storage protein family which is associated to severe reactions to food and could be highly cross-reactive. We pointed out specific mustard allergens that could be associated to severe reactions such as facial quincke edema with respiratory distress. [less ▲]

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See detailRare Sulfamethoxazole Crystalluria – case report
Castiglione, Vincent ULiege; Diop, Coumba Souley ULiege; CAVALIER, Etienne ULiege et al

Poster (2017, November 24)

Case discussion We report here 7 Caucasian patients with very uncommon crystalluria. There were 3 female and 4 male of 54 to 86 years-old. Patients had no relevant medical record in common. However, they ... [more ▼]

Case discussion We report here 7 Caucasian patients with very uncommon crystalluria. There were 3 female and 4 male of 54 to 86 years-old. Patients had no relevant medical record in common. However, they all were hospitalized for different types of infections: three patients had urinary infection, two had osteitis, and the two last had sepsis. The patients had all been first treated with different antibiotherapy, which had then been replaced by cotrimoxazole after antibiogram. The administrated doses varied from 800mg to 4800mg a day of Sulfamethoxazole. Crystalluria description In all patients, the urine microscopic analysis revealed unusual crystals of various shapes including rectangles, thick parallelepipeds, truncated lozenges, spheroids, mushrooms, or “flowers”. Some crystals were incorrectly identified by the urinary sediment analyzer as uric acid, but we sought to determine them accurately. Most of the crystals were strongly birefringent and measured between 20 and 100µm. Urine pH varied from 5.0 to 6.5 on strip analysis. After urine centrifugation, we performed infrared spectrophotometry analysis on dried residue. In all cases, the infrared spectra allowed us to identify the N-acetyl-Sulfamethoxazole, the main metabolite of Sulfamethoxazole. Crystalluria was observed between 1 and 26 days after Sulfamethoxazole treatment initiation. The serum creatinine increased from 16% to 88% in 3 patients between the first day of Sulfamethoxazole treatment and the day of crystalluria. These considerations raised concern for drug implication in renal failure in some of these patients. Teaching points for the clinical condition Drug-induced kidney failure is well-known, but the direct precipitation of drug crystals into tubules is rare, and also probably under-evaluated. Even if Sulfamethoxazole tubular precipitation was probably not the main cause of renal failure in these cases, we suspect it could have played a role. N-Acteyl-Sulfamethoxazole can precipitate in urine in many uncommon crystals shapes that raise suspicion for drug nephrotoxicity. Automated urine analyzers may misidentify these rare crystals. Crystal’s recognition may be difficult even with polarized light microscopy. This is why they must be identified by infrared spectrophotometry to avoid misdiagnosis. These renal failures linked to Sulfamethoxazole precipitation are more susceptible to appear with high dosage of drug, hypovolemia and pre-existing renal failure. Hypoalbuminemia has also been described as a risk factor and was present in our four patients (between 26 to 39g/l, range laboratory: 43-54). Thus, prevention of Sulfamethoxazole precipitation consists in hydratation to maintain urine flow, and require adaptation of cotrimoxazole dosage in regard of renal function. Urine alkalinization (pH >7.0) is also possible in order to increase Sulfamethoxazole metabolite solubility. [less ▲]

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See detailIntellectual and social enrichement linked to larger hippocampal volume in healthy aging
Narbutas, Justinas ULiege; Van Egroo, Maxime ULiege; Besson, Gabriel ULiege et al

Poster (2017, November 24)

Introduction. Decreased hippocampal volume in older adults is associated with episodic memory decline and subsequent neurodegenerative diseases. According to the dynamic polygon hypothesis, strategies ... [more ▼]

Introduction. Decreased hippocampal volume in older adults is associated with episodic memory decline and subsequent neurodegenerative diseases. According to the dynamic polygon hypothesis, strategies that increase neurogenesis of the hippocampus are likely to be successsful in delaying the onset of cognitive impairment in ageing. Several modifiable factors can have a positive effect on the size of the hippocampus, one of them being cognitive reserve. However, to date, very few studies reported an impact of cognitive reserve on hippocampal volume in healthy older adults. Therefore, the main objective of our study was to explore whether cognitive reserve is linked to hippocampal volume in healthy aging. We focussed particularly on intellectual and social enrichment during lifespan, because these aspects have been linked to hippocampal volume in clinical populations. Methods. Twenty-six healthy late middle-aged participants (51-69 y.o.) underwent 3T magnetic resonance imaging. Hippocampal volume was calculated with the Automatic Segmentation of Hippocampal Subfields (ASHS) software, which uses T1-weighted and T2-weighted MRI to obtain optimal segmentation of the hippocampus and its subfields. Raw volumetric scores obtained with ASHS were controlled for age and total intracranial brain volume. Only the main hippocampal regions (CA1, CA2, CA3, dentate gyrus) were included in the analysis. Volunteers also completed a questionnaire quantifying their lifespan engagement in intellectual (i.e. reading, hobbies) and social (i.e., volunteering, social games) enrichment. More specifically, participants had to describe the frequency of each activity they have engaged in from 6 years old to the present day. Results. Pearson correlation and hierarchical linear regression analyses revealed that higher frequency of intellectual (r = 0.40; p = 0.023) and social (r = 0.44; p = 0.013) enrichment was significantly linked to larger hippocampal volume, even when controlling for age and sex. Education, another proxy of cognitive reserve, had, however, no significant association with hippocampal volume, possibly due to restricted variance in education and small sample size. Conclusion. These results suggest that in a late middle-aged population, lifespan intellectual and social enrichment is related to larger hippocampal volume. These findings could indicate that lifespan enrichment promotes hippocampal neurogenesis. Future analysis on a larger sample will distinguish the impact of early and later life enrichment on hippocampal volume and will also assess whether this relation can modulate hippocampal-related changes in episodic memory in aging population. [less ▲]

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See detailOral calcium load test for recurrent calcium stone-formers
Castiglione, Vincent ULiege; CAVALIER, Etienne ULiege

Poster (2017, November 24)

Background Calcium is the most frequent component of urinary stones, and hypercalciuria is the main risk factor in recurrent stone-formers. The oral calcium load test is a dynamical biological test that ... [more ▼]

Background Calcium is the most frequent component of urinary stones, and hypercalciuria is the main risk factor in recurrent stone-formers. The oral calcium load test is a dynamical biological test that determines the origin of hypercalciuria in order to optimize the treatment. However, there is little literature about it, and it seems to have lost popularity in daily practice, this why we studied a population of stone-formers who underwent the oral calcium load test. Methods Between 2013 and 2016, we prospectively recruited 117 recurrent calcium stone-formers. After 2 days of calcium restricted-diet, patients had urinary and blood sampling at baseline and 120 min after the intake of 1 g of calcium per os. Blood and urinary parameters were assessed during the dynamical test, including stone risk factors, calcium metabolism and bone evaluation. According to these results, patients were classified in three groups: resorptive, renal or absorptive hypercalciuria. Results First, 19 patients were diagnosed with normocalcemic primary hyperparathyroidism, assessed by inappropriate parathormone decrease (41.41±12.82 vs. 54.06±13.84% p<0.01) in regard to calcemia. The measurement of ionized calcium was mandatory in order to detect induced hypercalcemia after calcium intake. These patients also had higher beta-crosslaps, lower phosphate reabsorption threshold and lower distal third radius bone mineral density. The treatment of this first group of patient is the hyperparathyroïdectomy. Fasting hypercalciuria was present in 39 patients with urinary calcium >0.37mmol/mmol of creatinine, and without hyperparathyroidism, classified thus as renal hypercalciuria. The treatment of these patients should include adapted calcium intake and thiazids. The third group included 34 patients with absorptive hypercalciuria defined by the presence of delta urinary calcium/creatinine <0.6mmol/mmol between 0 and 120 min, and without any other significant abnormality. Finally, the test result was not reliable for 33 cases because of the absence of sufficient calcemia increase or when the cause of lithogenesis could not be clearly identified. Conclusions The oral calcium load test was successful for the identification of main metabolic conditions leading to urolithiasis, including normocalcemic primary hyperparathyroidism, and is useful to improve and personalize the treatment of stone-formers. [less ▲]

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See detailTrophic ecology of icefishes (Notothenioidei, Perciformes) in a context of climate change
Peignot, Quentin; Frederich, Bruno ULiege; Lepoint, Gilles ULiege et al

Poster (2017, November 23)

The Southern Ocean currently undergoes major environmental modifications related to climate change. When faced with environmental changes, all organisms are expected to have some intrinsic capacity to ... [more ▼]

The Southern Ocean currently undergoes major environmental modifications related to climate change. When faced with environmental changes, all organisms are expected to have some intrinsic capacity to adapt their ecological habits to their new life conditions. Currently, biological traits of many Antarctic species are still unknown and we need such information to assess their capacity to adapt to natural and anthropogenic perturbations. Notothenioidei (Perciformes), or icefishes, are endemic to the Southern Ocean and they are an important trophic component of these marine ecosystems. Here we used stable isotopes ratios of carbon (δ13C) and nitrogen (δ15N) of two well represented genera of icefishes (Trematomus and Lepidonotothen) sampled in 2002/2004 and/or in 2015/2016 to better understand their resources partitioning and the influence of environmental changes on their trophic ecology. Isotopic niches (which are a proxy of the realized ecological niches) were modeled using the SIBER (Stable Isotope Bayesian Ellipses in R) R-package. Our results revealed a great variation in isotopic compositions among icefishes, suggesting that they exploit a wide array of resources. The overlap between the isotopic niches of Trematomus and Lepidonotothen genera in 2002/2004 highlights a possible past competition for resources. Moreover, temporal evolution of niches suggests taxon-specific ecological plasticity in response to variation in environmental parameters and/or in prey availability. An increase in resources partitioning between the two genera was observed over the studied period. In the future, it would be interesting to use mixing models including isotopic composition of potential preys to identify possible changes in their trophic ecology. [less ▲]

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See detailMorphological diversification of the endemic Antarctic fishes Trematominae (Notothenioidei, Teleostei)
Frederich, Bruno ULiege; Heindler, Franz Maximilian; Christiansen, Henrik et al

Poster (2017, November 23)

Adaptive radiation involves the early, rapid ecological and morphological diversification of multiple lineages from a common ancestor into new, diverging adaptive zones. Regarding this definition with ... [more ▼]

Adaptive radiation involves the early, rapid ecological and morphological diversification of multiple lineages from a common ancestor into new, diverging adaptive zones. Regarding this definition with emphasis on the tempo of diversification, Antarctic notothenioids represent one of the very few examples of adaptive radiation in marine fishes. Time-calibrated phylogenies suggest that the diversification of most speciose notothenioid lineages occured between 20 and 15 Ma. The subfamily Trematominae is one of those diverse subclades showing a large range of ecological niches. In the present study, we aim to describe the evolutionary history of Trematomus species. By combining a consensus time-tree and a geometric morphometric dataset, we first illustrate their pattern of cephalic shape diversification in a phylomorphospace and we infer the morphology of their common ancestor. We also explore whether the cephalic shape data shows a phylogenetic signal, which is defined as the statistical dependence among species trait values due to their phylogenetic relatedness. The combination of phylogenetic signal test and exploration of the phylomorphospace allows us to assess whether Trematomus rapidly diverged in various adaptive zones as expected under a classic scenario of adaptive radiation. [less ▲]

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See detailEffects of fluid preservation on sea star stable isotope compositions: How useful can museum collections be for trophic ecology studies?
Le Bourg, Baptiste ULiege; Lepoint, Gilles ULiege; Michel, Loïc

Poster (2017, November 23)

Stable isotope analyses (SIA) of carbon (δ13C) and nitrogen (δ15N) are a common tool to investigate the trophic ecology of animals. After field sampling, animal tissues are usually preserved dried or ... [more ▼]

Stable isotope analyses (SIA) of carbon (δ13C) and nitrogen (δ15N) are a common tool to investigate the trophic ecology of animals. After field sampling, animal tissues are usually preserved dried or frozen before subsequent analysis. However, museum samples, or samples not initially collected for SIA are often fixed in preservative solutions. These preservation methods may alter stable isotope ratios in animal tissues, and those effects should be quantified. Here, we investigated long-term effects (one year) of four preservation methods (freezing, alcohol, formaldehyde, drying) on δ13C and δ15N values of sea stars. We show that only formaldehyde preservation significantly affects δ13C values. However, after being altered, the mean δ13C value remained stable over time, suggesting that a correction factor may be used regardless of preservation time. δ15N values change across time for all methods except freezing but this may be the result of a higher intra-individual variability of δ15N values. The influence of preservation methods on estimation of isotopic niches size (parameterised as standard ellipse area) was also investigated. Bayesian estimations of standard ellipse areas (SEAB) of preserved samples were not different from those of fresh samples except for the samples preserved 6 months in alcohol. These preliminary results suggest that samples stored during one year in preservative fluids may be used for SIA in order to investigate the trophic ecology of sea stars. Future results will also help to determine whether the findings can be generalised to sea stars preserved for a longer time, such as those in museum collections. [less ▲]

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See detailAdductor mandibulae muscle and lower jaw morphology: an approach to determine bite strategies in piranhas and relatives (Serrasalmidae, Characiformes, Teleostei)
Huby, Alessia ULiege; Parmentier, Eric ULiege

Poster (2017, November 23)

Serrasalmidae (98 species) is a large monophyletic group of South American freshwater fishes. They have different dietary preferences ranging from the predominantly herbivorous or frugivorous pacus to the ... [more ▼]

Serrasalmidae (98 species) is a large monophyletic group of South American freshwater fishes. They have different dietary preferences ranging from the predominantly herbivorous or frugivorous pacus to the omnivorous or primarily carnivorous piranhas. Flesh-eating piranhas are supposed to have proportionally the most powerful bite forces among vertebrates but it has never been compared with those of plant, fruit and seed-eating species. Moreover, the bite strategy has never been explored in this family: is there a difference between carnivorous piranhas and herbivorous relatives? In the present study, we used the adductor mandibulae muscle and the lower jaw as models to answer this questioning through morphometric methods. We found that flesh-eating piranhas have a more developed adductor mandibulae muscle than seed-eating species which in turn have a larger jaw muscle than plant-eating species. The dorsomedial section of the jaw muscle (pars malaris) is the most developed in carnivorous species whereas it is the ventrolateral section (pars rictalis) in herbivorous species. We also distinguish two characteristic shapes of lower jaw that are related to dietary preferences. Results suggest different bite strategies: flesh-eating species possess a "scissor-like" way of feeding whereas the plant, fruit and seed-eating species show "vise-like" system. [less ▲]

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See detailAgroforestry in temperate regions, where does the water go? Electrical resitivity tomography as a tool to help us find out.
Maloteau, Sophie; Coussement, Tom; Pardon, Paul et al

Poster (2017, November 23)

Recently, agroforestry systems have been recognized to provide an opportunity for “ecological intensification”, thereby increasing yield outcome while simultaneously minimizing negative impacts on the ... [more ▼]

Recently, agroforestry systems have been recognized to provide an opportunity for “ecological intensification”, thereby increasing yield outcome while simultaneously minimizing negative impacts on the environment. Mixtures of trees and crops have the potential to capture more resources of light, water and nutrients than monocultures of trees or crops (Cannell et al. 1996). Nevertheless, few studies are available focusing on the impact of trees on soil moisture dynamics in cropped soil in temperate regions. In this study, we monitored the soil water dynamics in a corn field bordered by poplar trees in Ieper, Belgium using Electrical resistivity tomography (ERT) and classical soil tension sensors (Watermark) during the entire growing season of 2016 (May-September). We installed four ERT transects of 30 m long with an electrode spacing of 50cm. Three transects were placed in a part of the field bordered by trees and one reference transect was located in a part of the field without trees. Next to each transect, Watermark sensors were installed to estimate the soil water tension. The data allow us to monitor the influence of the trees on the soil water depletion by the crop. We quantified the effect in space and time of mature poplar trees on soil moisture dynamics in an agricultural field sown with maize during one growing season and confirmed the ability of electrical resistivity tomography to study tree-crop interactions for water under field conditions and we delimited an area of influence of the tree on the crop using a segmented linear regression technique. With our study, we show the potential of ERT to quantify tree-crop-soil interactions for water in agroforestry systems. [less ▲]

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See detailA forward model for electrical conduction in soil-root continuum: a virtual rhizotron study
Rao, Sathyanarayan; Meunier, Félicien; Ehosioke, Solomon ULiege et al

Poster (2017, November 23)

Electrical Resistivity Tomography monitoring of soil-root system water fluxes have received growing interest in the past decades. Some studies suggest that roots can be more electrically conductive than ... [more ▼]

Electrical Resistivity Tomography monitoring of soil-root system water fluxes have received growing interest in the past decades. Some studies suggest that roots can be more electrically conductive than soil. We suggest that ERT data taken in agricultural fields is impacted by plant roots and might contaminate estimates of soil water content based on bare soil petrophysical relations. To understand how do roots impact electrical current flow and thus ERT data, a numerical electrical model was coupled with a mechanistic maize-soil water flow model. All the maize roots with a radius larger than 0.05 cm were explicitly accounted for in the finite element mesh and associated to their specific electrical properties. Root growth and water uptake processes continuously affected the EC contrast between soil and root. We demonstrated that high contrats between root and soil EC lead to errors in the estimation of soil water content, which could be disminished by using an appropriate biopedophysical correction term. The effective EC (bulk properties) of the medium computed using simulated plate electrodes at rhizotron boundaries reveal directional anisotropy induced by root processes and is more pronounced in sand medium when compared to loam. The percentage change in bulk EC due to change in direction (𝐸𝐸𝐸𝐸ℎ𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜𝑜 .vs. 𝐸𝐸𝐸𝐸𝑣𝑣𝑣𝑣𝑣 𝑣 𝑣𝑣𝑣𝑣𝑣 𝑣 ) starts at ~30 % in sand and ~3 % in loam when root is young and increases upto ~500% in sand and ~20% in loam at day 22 when root is three weeks old. Directions in which there is more anisotropy contains more information on the root processes and hence they can be used as prior information for ERT injection scheme to retrieve better information. [less ▲]

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See detailEvaluation of leaf area index of winter wheat canopy by means of ground-based stereoscopic vision
Leemans, Vincent ULiege; Glésener, Michaël; Dumont, Benjamin ULiege et al

Poster (2017, November 22)

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See detailLes biais attentionnels envers l’alcool chez les « petits consommateurs » vs « gros consommateurs » dans un environnement virtuel
Pollet, Hélène; Quertemont, Etienne ULiege; Bouchard, Stéphane et al

Poster (2017, November 18)

Introduction : Les personnes dépendantes à l'alcool montrent des biais attentionnels quand elles sont confrontées à des indices liés à l’alcool (Kreusch, Vilenne, & Quertemont, 2013). D’après le modèle de ... [more ▼]

Introduction : Les personnes dépendantes à l'alcool montrent des biais attentionnels quand elles sont confrontées à des indices liés à l’alcool (Kreusch, Vilenne, & Quertemont, 2013). D’après le modèle de Wiers et al. (2007), les biais attentionnels favoriseraient la consommation répétée de substances. Objectif : Cette étude exploratoire évalue dans quelle mesure la réalité virtuelle fournit un environnement permettant de tester ces biais attentionnels. Hypothèse : Les gros consommateurs d'alcool présentent des biais attentionnels plus fréquents et plus intenses que les petits consommateurs. Méthode : Un questionnaire en ligne permettant de recruter 24 petits et 24 gros consommateurs d’alcool a d’abord été administré afin de présélectionner les sujets. Les participants ont ensuite réalisé une tâche d’immersion dans un environnement virtuel de « bar ». Le nombre de zooms effectués sur les stimuli « bières » et le nombre de commentaires rapportés en lien avec l’alcool étaient mesurés. Directement après l’immersion, la Visual Analogue Craving Scale (VAS) leur était administrée afin d’évaluer leur envie de boire. Résultats : Les « gros consommateurs » présentent davantage de zooms sur les stimuli « bières » que les «petits consommateurs». De plus, les « gros consommateurs » présentent un score plus élevé à la VAS que les «petits consommateurs », ce qui témoignerait d’une envie de consommer plus élevée après l’immersion par rapport aux « petits consommateurs ». Cependant, aucune corrélation significative n’a été mise en avant entre le nombre de zooms effectués et le score à la VAS chez les « gros consommateurs ». Conclusion : En accord avec nos hypothèses, nos résultats ont montré que les gros consommateurs présentent davantage de biais attentionnels envers les stimuli liés à l’alcool que les petits consommateurs ». Cependant, de futures recherches sont nécessaires afin de pouvoir confirmer que la réalité virtuelle fournit un environnement qui permet de tester les biais attentionnels. [less ▲]

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See detailUtilisation de la réalité virtuelle comme outil thérapeutique Quels sont les facteurs influençant son acceptation auprès des cliniciens ?
Marchal, Sylvie; Etienne, Anne-Marie ULiege; Willems, Sylvie ULiege et al

Poster (2017, November 18)

Le travail d’un psychologue, d’un neuropsychologue ou encore d’un logopède évolue constamment en fonction des apports de la recherche. L’efficacité de la réalité virtuelle comme outil thérapeutique est ... [more ▼]

Le travail d’un psychologue, d’un neuropsychologue ou encore d’un logopède évolue constamment en fonction des apports de la recherche. L’efficacité de la réalité virtuelle comme outil thérapeutique est étudiée et soulignée depuis une vingtaine d’années (Malbos et al., 2013). Néanmoins, actuellement, une minorité de cliniciens l’ont intégrée dans leur pratique (Gicquel, 2016). Le but de cette étude est d’identifier les facteurs ayant un rôle déterminant dans le choix des cliniciens d’utiliser la réalité virtuelle. Pour ce faire, nous avons effectué notre travail en deux étapes : l’élaboration d’un modèle conceptuel et l’élaboration d’un questionnaire visant à éprouver la pertinence du modèle proposé. Le modèle a donc été construit sur base de la littérature concernant l’acceptation d’une technologie (Azjen, 1985 ; Davis, 1989 ; Taylor et Todd, 1995 ; Venkatesh et al., 2008). Il soutient l’idée que l’intention de réaliser un comportement dépendrait de l’utilité perçue, de l’attitude, des normes subjectives et de la perception de contrôle comportemental. Ce modèle a ensuite guidé l’élaboration des items de notre questionnaire en adaptant ceux régulièrement proposés au sein de la littérature (Davis, 1989 ; Glegg, 2013 ; Venkatesh et al., 2008). Au niveau méthodologique, la diffusion du questionnaire s’est faite par internet au travers des réseaux sociaux et professionnels francophones. La participation se réalisait sur base volontaire. Ainsi, 98 personnes ont participé à notre étude (80 femmes, 18 hommes). Cet échantillon était composé de 71 psychologues (72,4%), 15 neuropsychologues (15,3%) et 12 logopèdes (12,2%). Parmi eux, 84 répondants (85,7%) n’avait jamais utilisé la réalité virtuelle dans un contexte clinique et plus de la moitié de l’échantillon s’estimait peu familier avec cette technologie ou les recherches sur son efficacité en clinique. En accord avec nos hypothèses, nos résultats indiquent que les cliniciens tendent principalement à se référer à la norme subjective, à leur perception de contrôle sur l’utilisation de l’outil ainsi qu’à leur attitude propre envers la technologie pour former leur intention d’utiliser la réalité virtuelle dans leur pratique clinique. Par contre, l’absence de significativité de l’utilité perçue est en désaccord avec notre hypothèse. Ceci pourrait potentiellement s’expliquer par la non-familiarité des répondants avec l’utilisation de l’outil virtuel. [less ▲]

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See detailFirm Performance and Multi-Scale Territorial Resources in Wallonia: Towards a Model using Business Accounting?
Wilmotte, Pierre-François ULiege; Halleux, Jean-Marie ULiege

Poster (2017, November 17)

In Europe (and in Wallonia), most public policies related to regional development and economic development aim to stimulate/reinforce the ‘’territorial resources’’ in order to improve the competitiveness ... [more ▼]

In Europe (and in Wallonia), most public policies related to regional development and economic development aim to stimulate/reinforce the ‘’territorial resources’’ in order to improve the competitiveness of the (Walloon, Belgian or European) firms. For instance, in Wallonia, one of the key action of the Marshall Plan (i.e. the main regional strategy in the domain of economic development) aims to “mobilize the territory” (in French, « mobilisation du territoire à destination du développement économique »). In this perspective, our objective is to quantify and analyse the territorial resources that impact the performance of Walloon or Belgian firms, with the final aim to advice the policy makers and to improve the related public policies. This topic is related to questions such as: Does a better accessibility of economic estates improve the performance of firms? Are cultural amenities (in order to attract talents) and land prices (in order to reduce their costs) strategic competitive advantages for firms? The poster describes some methodological issues of (in general) the PhD research and (particularly) one part of the research, concerning a multivariate statistics analysis of business accounting. [less ▲]

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See detailVers l'identification non-linéaire des structures industrielles au moyen de sinus balayés
Dossogne, Tilàn ULiege; Noël, Jean-Philippe ULiege; Kerschen, Gaëtan ULiege

Poster (2017, November 16)

Les phénomènes vibratoires non-linéaires sont fréquemment rencontrés dans les structures mécaniques et aérospatiales. La division de ces structures en sous-systèmes assemblés ou l’usage de nouveaux ... [more ▼]

Les phénomènes vibratoires non-linéaires sont fréquemment rencontrés dans les structures mécaniques et aérospatiales. La division de ces structures en sous-systèmes assemblés ou l’usage de nouveaux matériaux favorisent l’apparition de mécanismes non-linéaires tels que le frottement, les impacts ou l'hystérésis. Il existe donc un besoin croissant en industrie de pouvoir détecter et identifier ces phénomènes non-linéaires sur des structures complexes et à grande échelle. L’ objectif de ce travail est d'établir une méthodologie d’identification des non-linéarités dans les structures industrielles, allant de la détection à la caractérisation et l’estimation de paramètres non-linéaires. Le processus d’identification est basé uniquement sur des mesures obtenues en sinus balayé, afin d’ obtenir une séparation naturelle des modes ainsi qu’une excitation suffisante des mécanismes non-linéaires. La première étape, la détection, permet de localiser spatialement les sources de non-linéarités dans une structure au moyen d’un indice de non-linéarité. La seconde étape, la caractérisation, offre une visualisation qualitative des comportements non-linéaires locaux en utilisant la méthode dite ASM (Acceleration Surface Method). Cette méthode repose sur l’approximation des forces de rappel non-linéaires au moyen de signaux d’accélération représentés dans l’espace d’état. La dernière étape a recours à la méthode x-ASM (eXtended Acceleration Surface Method) afin d’estimer la valeur de chaque paramètre linéaire du modèle grâce à des procédés d’optimisation couplés à des modèles éléments finis. L’ensemble de la méthodologie est validée sur un avion F-16 Fighting Falcon possédant des non-linéarités au niveau des connexions entre les ailes et la charge utile. [less ▲]

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See detailEnzymatic inter-esterification of binary blends containing Irvingia gabonensis seed fat to produce Cocoa Butter Substitute (CBS)
Yamoneka Wasso, Juste ULiege; Malumba Kamba, Paul ULiege; Danthine, Sabine ULiege et al

Poster (2017, November 15)

Irvingia gabonensis seeds are rich in lipids. The fat extracted from these seeds is a lauric fat, that could have potentialities in confectionery applications. In the present study I. gabonensis seed fat ... [more ▼]

Irvingia gabonensis seeds are rich in lipids. The fat extracted from these seeds is a lauric fat, that could have potentialities in confectionery applications. In the present study I. gabonensis seed fat (IGF) was evaluated as possible Cocoa Butter Alternative. To this end, IGF was blended with four liquid oils, those blends were further enzymatically interesterified (EIE) to improve physicochemical properties. The EIE blend with Dacryodes edulis pulp oil (DPO) as liquid oil, was selected as the best candidate. Iso-solid diagram was then constructed, in order to study the compatibility between this new EIE-blend and cocoa butter (CB). The SFC melting profile of IGF can be compared to that of cocoa butter (CB), but the melting point of IGF is higher. After blending IGF with DPO (90-10) followed by an enzymatic interesterification, a new interesting fat is obtained, which presents a melting profile close to that of tempered CB and that is stable in β’1. No tempering is needed to induce a stable conformation of the obtained fat, contrarily to CB. The new fat can be blended with CB, but their compatibility is low, as indicated by the iso-solid diagram. This suggests that a maximum of 5% of the new fat could be added to CB and that this fat could be suitable as CBS for chocolate compound and confectionery fillings. [less ▲]

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See detailSowing flower strips in a wheat field to enhance biological control of aphids and support pollinators
Amy, Clara ULiege; Brigode, Mélanie ULiege; Hatt, Séverin ULiege et al

Poster (2017, November 14)

Insect pests are responsible, among other factors, for reducing the productivity of crops. While chemical insecticides used to control them cause harmful effects on human health and the environment ... [more ▼]

Insect pests are responsible, among other factors, for reducing the productivity of crops. While chemical insecticides used to control them cause harmful effects on human health and the environment, conservation biological control, i.e. managing habitats in agricultural landscapes to support pest natural enemies, turns out to be a promising approach. Moreover, the decline of pollinators in agricultural areas is observed since a few decades, being partly due to the intensification of agriculture which homogenizes and degrades agricultural landscape. To reduce the detrimental effects of agricultural intensification on biodiversity, ecosystem functioning and ecosystem services, farmers have the opportunity to adopt agri-environmental measures. Among them, the establishment of flower strips, with a recognized interest in biological control and the conservation of pollinators, is proposed. However, their establishment is not systematically efficient and profitable for the farmers. Adapting mixture composition to farmer’s need may encourage their adoption. Thus, the research project has a double goals (i) to provide intercropping flower strips for promoting the attraction of beneficial insects against pests and for promoting pollinators diversity; and (ii) to suggests diversification of farm income by planting monospecific oilseed flower strips. [less ▲]

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See detailCirculating Extracellular Vesicles From Mouse and Rat Models of Diabetes Reveal Specific Microrna Signatures as Biomarkers of Diabetic Cardiomyopathy
Chandy, Mark; Njock, Makon-Sébastien ULiege; Veitch, Shawn et al

Poster (2017, November 14)

Background: Type-2 diabetes (T2D) is associated with both reduced and preserved ejection fraction heart failure. Obese db/db mice and Goto Kakizaki (GK) rats represents animals models of T2D that develop ... [more ▼]

Background: Type-2 diabetes (T2D) is associated with both reduced and preserved ejection fraction heart failure. Obese db/db mice and Goto Kakizaki (GK) rats represents animals models of T2D that develop cardiac dysfunction similar to human diabetic cardiomyopathy, in which dominant early findings are of diastolic (and not systolic) dysfunction. Circulating extracellular vesicles (EV) contain microRNAs (miR) that can be transferred to recipient cells to modulate their function. We explored whether analysis of EV content from animals models of T2D would inform on the pathophysiology, diagnosis and therapeutic targets of cardiac dysfunction. Hypothesis: EV from animal models of T2D will have altered miR content that contributes to the pathophysiology of diabetic cardiomyopathy. Methods & Results: miR qPCR arrays on circulating EV isolated from plasma of db/db mice reveal several miR (-7, -15, -25, -30e, -148a, -150, -195) modulated during disease progression. These changes in miR content occur prior to echocardiographic evidence of diastolic dysfunction, including global longitudinal strain and strain rate. Among circulating EV miR from the GK rat model, miR-30 was also upregulated (1.42 fold, p=0.03) compared to Wistar rat. In GK rat left ventricle, and in H9C2 rat cardiac myoblast cultured in 25 mM high glucose media, mass spectrometry revealed proteins that were overexpressed in the diabetic heart including oxidative phosphorylation, glycolysis, fatty acid degredation and the citrate cycle. Using a bioinformatics approach, we next identified metabolic pathways affected by miR-30. Based on these findings, in vivo therapy with antagomiR and mimics of miR-30 are underway to test causality and reversibility of the observed cardiomyopathy. Conclusion: EV from animal models of T2D have altered miR content, including miR-30. We also identify alterations in the expression of a network of metabolism genes in the heart, which are implicated in diabetic cardiomyopathy. If causality is supported by experiments that enhance or block miR-30 expression in these models of disease, we will have identified a novel biomarker and therapeutic target for diabetic cardiomyopathy. [less ▲]

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See detailRapid changes in preoptic estradiol concentration during male sexual behavior
de Bournonville, Marie-Pierre ULiege; de Bournonville, Catherine; Ball, Gregory et al

Poster (2017, November 11)

Estrogens such as estradiol (E2) exert pleiotropic effects on physiological and behavioral responses such as neuroprotection, aggression or reproduction. Estrogens derived from local brain synthesis ... [more ▼]

Estrogens such as estradiol (E2) exert pleiotropic effects on physiological and behavioral responses such as neuroprotection, aggression or reproduction. Estrogens derived from local brain synthesis (neuroestrogens) are critical for the regulation of different functions including the control of male sexual behavior. Classically, E2 acts through effects initiated in the nucleus to regulate male sexual function. Along with these long-term effects, E2 also acts rapidly (within minutes) via membrane-initiated events. These effects are thought to depend on short-term variations in the local production of estrogens, through rapid fluctuations of the enzymatic activity of brain aromatase. In Japanese quail, rapid modulations of brain aromatase activity (AA) have been reported after sexual interactions or exposure to an acute stress. These changes take place mainly in the medial preoptic nucleus (POM), a sexually differentiated structure that plays a key role in the control of male sexual behavior and where aromatase is densely expressed. Yet, it has recently been shown that, in the short term, AA does not always reflect local E2 concentration. This study was designed to determine by in vivo microdialysis whether local E2 concentrations fluctuate during sexual interactions and test whether these changes parallel the decrease in AA observed ex vivo after copulation. We first conducted a series of experiments to validate the microdialysis and E2 assay. When dialysis probes were placed in successive baths containing known increasing amounts of E2, proportional changes in E2 concentration were measured in the dialysate. Moreover, a rise in E2 concentration was detected after in vivo retrodialysis of testosterone only if the probe was located within the POM and, after a peripheral injection of E2, a sharp rise of E2 was detected regardless of the probe location. Together these results show that in vivo microdialysis is a valid method to assess endogenous fluctuations of brain E2 concentrations in behaving animals. Two independent experiments then identified a rise in E2 concentrations in POM during sexual interactions. This increase occurred within 10 min after the initiation of the sexual interaction and was specific to the POM as there was no increase in E2 concentrations in males that had their cannula outside of this area. Together these data confirm that rapid changes in AA measured ex vivo cannot be considered as a reliable proxy for E2 concentrations. The discrepancies could originate either from the different time resolution related to the two techniques or from differences in the microenvironment in which aromatase functions in vivo and during ex vivo assays. [less ▲]

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See detailMise en évidence de l'activité neuronale par résonance magnétique fonctionnelle lors du port du froggy mouth: case report
PANS, Marine ULiege; BRUWIER, Annick ULiege; CHARAVET, Carole ULiege et al

Poster (2017, November 10)

Un appareil de rééducation de la déglutition atypique (interposition linguale entre les arcades dentaires lors de la déglutition) a été porté par un patient pour corriger sa béance dentaire antérieure ... [more ▼]

Un appareil de rééducation de la déglutition atypique (interposition linguale entre les arcades dentaires lors de la déglutition) a été porté par un patient pour corriger sa béance dentaire antérieure. Une IRMf a été réalisée avec et sans l'appareil afin de vérifier les zones de cerveau qui étaient stimulées car l'hypothèse est que l'appareil active les fibres motrices du nerf V. [less ▲]

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See detailImplementation of "Parler-Bambin" preventive program in the multilingual and multicultural context of nurseries/daycares in Lebanon
Moitel ép Messarra, Camille ULiege; Maillart, Christelle ULiege

Poster (2017, November 10)

This project is targeting prevention programs for language and communication difficulties in nurseries in Lebanon. More specifically, it will explore the implementation of a preventive program to enhance ... [more ▼]

This project is targeting prevention programs for language and communication difficulties in nurseries in Lebanon. More specifically, it will explore the implementation of a preventive program to enhance language skills in early childhood, and to specify the role of the Speech and Language Therapist (SLT) in this implementation. There is a growing consensus among researchers and clinicians that abilities acquired at an early age, especially in oral language, are predictive of children's academic performance and future social integration (Zorman, 2011). Preventive actions are increasingly recognized as a priority to improve public health worldwide (Law et al., 2013). In Lebanon, an ongoing national program (MHPSS 2015-2021 - MHPSS, Preventive and Curative, Ministry of Public Health, 2015) is taking part of this kind of healthcare development. Aims: Thus, taking into account some variables that affect the implementation process (environment communities, providers, prevention delivery system and the prevention support system), the aims of this project are 1) to study the feasibility of a preventive program model, that partners Speech and Language Therapist and educators or caregivers in daycare and nurseries, to facilitate language and communication in Lebanese toddlers (under 3 years old), 2) determine the success factors of the implementation. It will be based on a French program “Parler Bambin”, which is currently widely developed in France. Method: The first study will focus on the factors of engagement of different stakeholders in a prevention program: firstly, by identifying the professionals involved in nurseries in Lebanon and then identifying the characteristics of these stakeholders in diverse childcares. Data will be gathered through a questionnaire constructed or adapted from existing questionnaires for this purpose (eg, Preschool Teacher Literacy Beliefs Questionnaire [TBQ], Hindman and Wasik, 2008). Similarly, the questionnaires will address SLT’s representations about their role in supporting language in nurseries. Nominal groups or focus groups (Baribeau and Germain, 2010) and questionnaires will be also carried out to identify the facilitators of this kind of preventive actions and the specific needs of SLTs in preventive settings. A second phase will target the implementation process itself: adapting and implementing a preventive intervention, while taking into consideration implementation aspects. We will find out major variables that are worthy for the program’s implementation, by measuring its fidelity (dosage, quantity, intervention strength, quality, participant responsiveness, program differentiation, etc.), also using videos and direct coaching sessions. Pre and posttest measures will be applied on children (MCDI -The MacArthur communicative development inventories, Fenson et al., 1996; Questionnaire for parents of bilingual children LITMUS-PABIQ, COST IS0804, validation in Lebanon, Tuller 2015). Finally, following the pilot study, the implementation of the adapted program will be proposed according to different execution modalities to support its implementation: with a direct or indirect involvement of an SLT. It will be tested more widely, with particular focus on the role of the speech therapist. Evaluation measures similar to those presented in Phase 2 will be carried out. Expected results: This project will highlight the added value of speech therapy in setting up a prevention program. It also participates in the elaboration of a national strategic framework for the Speech and Language Therapy [less ▲]

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See detailTeacher-SLT co-intervention program to support language in kindergartens in Lebanon: methodology, procedures and measures
El Kouba, Edith ULiege; Maillart, Christelle ULiege

Poster (2017, November 10)

Background: The aim of this project is to develop a co-intervention program, lead by a teacher and an SLT, to support oral language in preschoolers in the Lebanese context (multicultural, multilingual ... [more ▼]

Background: The aim of this project is to develop a co-intervention program, lead by a teacher and an SLT, to support oral language in preschoolers in the Lebanese context (multicultural, multilingual). Actually, there is a growing consensus, that efforts to enhance children’s early language skills, can improve their reading skills and long-term academic achievement. However, some children are at risk of delays due to diverse environments (e.g. low socioeconomic status), and impoverished language and literacy experiences. Thus, international recommendations highlight the necessity for SLTs to conceptualize their intervention, according to community based needs, focusing on primary prevention for early communication and language difficulties, given their later impact on social and professional integration (Law 2013). Aims: In line with the recommendations of the Ministry of Public Health in Lebanon (Mental Health program services MHPSS, preventive and curative, Ministry of Public Health, 2015), the purpose of the project is to support language skills in preschoolers by adapting, implementing and analyzing co-intervention program that partners early childhood educators along with SLTs. Methods: First, we will identify the beliefs, representations and expectations of the different stakeholders (teachers, speech-language pathologists, school principals) through questionnaires and interviews among the diverse Lebanese school contexts in order to 1) identify the knowledge and 2) gather their expectations in the area of support and prevention for language difficulties in kindergarten. Second, the project aims to identify the quality of teacher-child interaction, considered as one of the primary mechanism that foster the development of child’s language competences. It is commonly assessed by using the Classroom Assessment Scoring System (CLASS-K; Pianta, La Paro, & Hamre, 2008). Ten dimensions will be considered: climate (positive or negative), teacher sensitivity, children's perspectives, behavior management, productivity, teaching practices and learning formats, concept Development, quality of feedback provided to the child, and linguistic modeling. Finally, we will design a co-intervention program that is the most consistent with the contextual, cultural and linguistic diversity in Lebanon. Two intervention settings will be addressed and compared: 1) a co-intervention with an SLT including direct coaching, and video-retroactions and 2) an implementation of the program led by the teacher after a training provided by an SLT. We will collect information about children’s bilingual environment through the Parental Bilingual Questionnaire (PABIQ-LITMUS, COST Action IS804), and we will evaluate interactions through CLASS-K (Pianta, La Paro, & Hamre, 2008), pre and post evaluation. Children's language skills will be measured through: ELO-L (Zebib, Khomsi, Henry, Messarra, & Kouba Hreich, Lebanon Tests Editions, 2017). Moreover, measurements of the fidelity of program’s implementation (dosage, adhesion, quality of the interactions, responsiveness) will also be carried out. Results: The expected results are intended to demonstrate to which extent an intervention program, to enhance communication and language skills, carried out jointly by teachers and speech therapists, would be more effective than a program delivered without the intervention of SLTs. The intervention of speech-language pathologists would thus have a more effective impact on the teachers' practices and the language skills of the children. This would justify the primary role of SLTs in indirect interventions in preschools in Lebanon. [less ▲]

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See detailInjection of spin-polarized current in a Ge-based magnetic device with coplanar contacts
Fourneau, Emile ULiege; Avila Osses, Jonathan ULiege; Silhanek, Alejandro ULiege et al

Poster (2017, November 09)

Electron spin injection and spin detection in magnetic materials are key features to the functionalization of electron spin polarization as a degree of freedom for both information processing and storage ... [more ▼]

Electron spin injection and spin detection in magnetic materials are key features to the functionalization of electron spin polarization as a degree of freedom for both information processing and storage. Currently, spin-dependent tunneling in magnetic junction devices is the most common approach to achieve efficient spin injection. However, many recent studies highlighted the interesting possibility to create spin-polarized currents in structures which combine a magnetic semiconductor, e.g. magnetic alloys based on group-IV semiconductors or diluted magnetic semiconductor compounds, forming a Schottky-like rectifying junction with a metallic ferromagnet. Although theoretical works have already addressed the performance of this structure by numerical simulations of the spin drift and spin diffusion equations, taking into account various characteristics of the ferromagnet (FM) / semiconductor (SC) interface such as barrier height and boundary roughness in 1D models, correlations with experimental results are scarce. This work aims at achieving spin injection with Ge-based magnetic structures using a rectifying junction in a coplanar architecture. We performed 2D numerical calculations of the spin drift and diffusion process in the direct neighborhood of a junction consisting of a Mn5Ge3 half-metallic ferromagnet acting as the injecting contact and an n-type Ge film, forming 3- and 4-terminal devices. Our results show that geometrical effects play a major role on the spin injection efficiency. Moreover, the simulations emphasize the asymmetry of spin accumulation at the FM/SC interface as well as a strong effect of the depletion layer caused by the Schottky contact junction. [less ▲]

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See detailPE teachers as promotors of physically educated citizens: Comparison of their representations in Quebec and Wallonia
Cloes, Marc ULiege; Carbonneau, Ulysse; Grenier, Johanne

Poster (2017, November 08)

If physical education (PE) was sport oriented since the 80’s, an international movement underlined a need of change in order to deal more with the society evolution. Since the turn of the new millennium ... [more ▼]

If physical education (PE) was sport oriented since the 80’s, an international movement underlined a need of change in order to deal more with the society evolution. Since the turn of the new millennium, PE has been increasingly linked to the promotion of physically active lifestyles. The implementation of such change of core values differed dramatically from one country to another. For example, in two French speaking regions, Quebec and Wallonia, the educational stakeholders reacted in different ways. As soon as 2001, the Quebec Ministry of Education decided to entrust PE and health education to PE teachers while the Wallonian educational authorities seem to discover today the central role that PE teachers could play in promoting a healthy lifestyle in youth. In this study, we compared Quebec and Wallonian PE teachers’ representations of their role as promotors of an active lifestyle. Six Canadian (3 females) and 11 Wallonian PE teachers (6 females) answered to a semi-structured interview. It was focused on: students’ motivation and self-confidence to engage in regular physical activity; knowledge about the health benefits of an active lifestyle, and self-determination to practice regular physical activity. A content analysis of the verbatim was processed in order to identify the main concepts expressed by the subjects. At the current state of the data analysis, it appears that cultural differences are clearly identified between Canadian and Wallonian PE teachers. The development of a healthy active lifestyle is more predominant among Canadian educators who can draw their experiences in more than 10 years of the Physical and Health Education curriculum while their colleagues from Wallonia are still trying to test new approaches without a strong institutional support. Wallonian teachers could take benefit of their colleagues’ experience in order to facilitate the adoption of news teaching strategies. [less ▲]

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See detailModulation of NMDA receptor mediated excitatory synaptic currents in dopamine neurons of the substantia nigra.
Destreel, Geoffrey ULiege; Seutin, Vincent ULiege; Engel, Dominique ULiege

Poster (2017, November 08)

Dopamine (DA) neurons of the substantia nigra pars compacta (SNc) exhibit two main firing modes, spontaneous single action potential (AP) firing and bursting. The amount of DA released by these neurons in ... [more ▼]

Dopamine (DA) neurons of the substantia nigra pars compacta (SNc) exhibit two main firing modes, spontaneous single action potential (AP) firing and bursting. The amount of DA released by these neurons in target areas depends on the presynaptic AP firing pattern and is essential to modulate several aspects of behavior such as the control of movement. Bursting activity is mediated by excitatory afferents and specifically by the activation of NMDA receptors (NMDARs). However, the level of activation of NMDARs at these synapses during spontaneous synaptic activity is unknown. We assessed the occupancy of the glycine binding sites of the NMDAR by testing the effects of coagonists and by blocking the uptake of glycine on spontaneous excitatory postsynaptic currents (sEPSCs) in DA neurons of the SNc. [less ▲]

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See detailActive Healthy Kids Belgium 2016 Report Card on Physical Activity for Children and Youth
Seghers, Jan; Wijtzes, Anne I; De Ridder, Karin A.A. et al

Poster (2017, November 08)

This 2016 Belgium Report Card on Physical Activity for Children and Youth is the first systematic evaluation of physical activity behaviors, related health behaviors, health outcomes, and influences thereon ... [more ▼]

This 2016 Belgium Report Card on Physical Activity for Children and Youth is the first systematic evaluation of physical activity behaviors, related health behaviors, health outcomes, and influences thereon, using the Active Healthy Kids Canada grading framework (Tremblay et al., 2015). A research working group as well as policy experts from both Flanders and Wallonia collaborated to determine the indicators to be graded, data sources to be used, and factors to be taken into account during the grading process. Grades were assigned based on examination of the current data and literature for each indicator against a benchmark or optimal scenario: A (81-100%) = We are succeeding with a large majority of children; B (61 – 80%) = We are succeeding with well over half of children; C (41 – 60%) = We are succeeding with about half of children; D (21 – 40%) = We are succeeding with less than half, but some, children; F (00 – 20%) = We are succeeding with very few children; INC = there is no or insufficient evidence to assign a grade. In addition to an overall grade, an indicator could be assigned a plus sign or minus sign based on the presence or absence, respectively, of substantial social inequalities, according to age, region, gender, or socioeconomic status. Eleven indicators were selected and assigned the following grades: overall physical activity (F+), organized sport participation (C-), active play (C+), active transportation (C-), sedentary behaviors (D-), school (B-), government strategies and investment (C+), and weight status (D). Incomplete grades were assigned to family and peers, community and the built environment, and dietary behaviors due to a lack of nationally representative data. Despite moderately positive social and environmental influences, physical activity levels of Belgian children and youth are low while levels of sedentary behaviors are high. [less ▲]

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See detailERα- and dose-dependent effect of estetrol on angiogenesis and tumor growth
Gallez, Anne ULiege; BLACHER, Silvia ULiege; Gérard, Céline et al

Poster (2017, November 07)

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See detailRaman Chemical Imaging in Kidney Stone Analysis
Castiglione, Vincent ULiege; Sacre, Pierre-Yves ULiege; CAVALIER, Etienne ULiege et al

Poster (2017, November 02)

Background: The structure of kidney stones might provide clinical useful information in addition to the stone composition. The Raman chemical imaging (RCI) is a new technology used for the production of ... [more ▼]

Background: The structure of kidney stones might provide clinical useful information in addition to the stone composition. The Raman chemical imaging (RCI) is a new technology used for the production of two-dimensions maps of the constituents' distribution in samples. We aimed at determining the use of RCI in urinary stone analysis. Methods: Twelve calculi were analyzed by RCI using a confocal Raman microspectrophotometer. They were selected according to their heterogeneous composition and morphology. Prior to the analysis, samples were sliced and milled in order to detect the nucleus of the stones and having a smooth surface. RCI was performed on the whole section of stones. Once acquired, the data were baseline corrected and analyzed by MCR-ALS. Results were then compared to the spectra obtained by Fourier Transform Infrared spectroscopy, the gold standard method for the determination of urolithiasis composition. Results: RCI succeeded in identifying all the chemical components contained in each sample, including monohydrate and dihydrate calcium oxalate, anhydrous and dihydrate uric acid, apatite, struvite, brushite, whitlockite and ammonium urate. However, proteins couldn't be detected because of the huge autofluorescence background and the small concentration of these poor Raman scatterers. Carbapatite and calcium oxalate were correctly detected even when they represented less than 5 percent of the whole stones, allowing the detection of very small structures like Randall's plaques. Moreover, RCI provided the distribution of components within the stones. The nuclei were accurately identified, as well as thin layers of other components. Conversion of dihydrate to monohydrate calcium oxalate was correctly observed in the center of one sample. Conclusion: RCI showed a good accuracy in comparison with infrared spectroscopy in identifying components of kidney stones. In addition, RCI is nondestructive enabling the storage of samples. This analysis was also useful in determining the organization of components within stones, which help locating constituents in low quantity, such as nuclei. However, this analysis is time-consuming, which makes it more suitable for research studies rather than routine analysis. [less ▲]

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See detailComparison of faecal microbiota of horses suffering from atypical myopathy and healthy co-grazers
Cerri, Simona ULiege; Taminiau, Bernard ULiege; Votion, Dominique ULiege et al

Poster (2017, November 02)

Objectives: To characterize faecal microbiota of horses with atypical myopathy (AM) compared with healthy co-grazers (HcG). Methods: Fresh faecal samples were obtained from 6 horses (1 stallion, 3 ... [more ▼]

Objectives: To characterize faecal microbiota of horses with atypical myopathy (AM) compared with healthy co-grazers (HcG). Methods: Fresh faecal samples were obtained from 6 horses (1 stallion, 3 geldings and 2 females; mean age of 11.810 years) with confirmed AM and 6 HcG (4 geldings and 2 females; mean age of 13.68 years) during autumn-2016 and spring-2017 AM outbreaks in Belgium. Bacterial taxonomy profiling obtained by 16S amplicon sequencing of faeces was used to identify differentially distributed bacterial taxa between AM and HcG. Results were statistically compared using Welch's t-test with STAMP software. Results: A total of 90,407 sequences were analysed and clustered to 8,066 operational taxonomic units. Bacterial populations were distributed between 17 phylas, although 20% of sequences could not be attributed to an existing phylum. Horses with AM harboured a significantly higher relative abundance of Ruminococcaeae family with a significantly lower Lachnospiraceae when compared to HcG. Discussion: AM is caused by hypoglycin A intoxication, but only a part of horses pasturing in the same toxic environment develops the pathology, suggesting that there may be protective factors at the horse level. The results of this study show significant differences in faecal microbiota between AM cases and HcG, which could suggest that microbiota could play a role in the development or prevention of clinical disease. Conclusions: Results demonstrate that microbiota of AM affected horses is significantly different compared to HcG. Significance: Microbiome could influence the development of AM, but this role deserves further investigation. [less ▲]

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