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See detailAn analysis of potential resistance of the phytophagous mite, Tetranychus urticae Koch (Acari: Tetranychidae) to four botanical pesticides
Attia, Sabrine; Lebdi, KL; Heuskin, Stéphanie ULiege et al

in Biotechnologie, Agronomie, Société et Environnement (2015), 19(3), 232-238

Description of the subject. Synthetic acaricides have been widely used to manage Tetranychus urticae. Due to the excessive use of biocide and the associated problems of pesticide resistance and ... [more ▼]

Description of the subject. Synthetic acaricides have been widely used to manage Tetranychus urticae. Due to the excessive use of biocide and the associated problems of pesticide resistance and environmental pollution, there is an increasing demand for sustainable, environmentally-friendly control methods. Among the current alternative strategies aimed at decreasing the pest populations, the pesticides based on plant extracts are currently one of the most promising methods. Essential oils with acaricidal properties have been categorized as green pesticides because they are biodegradable and predominantly non-toxic to vertebrates. Objectives. With an aim to reduce the use of synthetic pesticides, they represent a promising approach for eco-chemical control of mites. Method. The aim of the present work was to analyze the risk of resistance emergence of T. urticae to repeated treatments with four plant extracts: Deverra scoparia Coss. & Durieu (Araliales: Apiaceae), Hertia cheirifolia (L.) Kuntze (Asterales: Ateraceae), Santolina africana Jord. & Fourr. (Asterales: Asteraceae) essential oils and garlic distillate Allium sativum L. (Asparagales: Alliaceae) after 20 generations. Results. Repeated treatments with S. africana essential oil during 20 generations did not provoke an emergence of resistance while a low development of resistance was observed with H. cheirifolia, A. sativum and D. scoparia extracts. Conclusions. The efficacy of these extracts against the two spotted spider mite and their low development of resistance make them a promising use for pest management. [less ▲]

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See detailAnalysis of preferences of agro-pastoralists for the attributes of traction dromedaries in harness cultivation: A case study of the Koro district of Mali
Traoré, Bakary ULiege; Govoeyi, Benoît ULiege; Hamadou, Issa et al

in Pastoralism (2019), 9(19),

The aim of this study was to characterize agro-pastoralists’ preferences in dromedary rearing in the Koro district of Mali, which has experienced a decade of spontaneous development in using dromedaries ... [more ▼]

The aim of this study was to characterize agro-pastoralists’ preferences in dromedary rearing in the Koro district of Mali, which has experienced a decade of spontaneous development in using dromedaries for harnessed cultivation. Five criteria for assessment of dromedary types used for traction were identified through nine focus group discussions. The assessment criteria developed and estimated rates were the morphological characteristics (23.2%), disease resistance (14.1%), work attitude (26.3%), colour of the coat (25.3%) and docility (11.1%). A multi-attribute analysis method was applied with 115 dromedary owners. Conditional logit was used to estimate utility function and willingness to pay (WTP) for different attributes. Work attitude seems to be an important parameter for decision-making with a WTP amounting to 558.94 euros (614.94 USD). Disease resistance comes second position in decision-making for the selection of dromedaries, with a WTP of 348.77 euros (383.40 USD). To a lesser extent, the white coat with a WTP in the order of 54.45 euros (59.86 USD) intervenes in agro-pastoral decision-making. The agro-pastoralists have a strong reluctance for the dark brown coat. In conclusion, the present results showed that further studies should be conducted on materials and traction technologies to promote harnessed cultivation by dromedaries to preserve and strengthen the multi-functional nature of the dromedary. [less ▲]

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See detailAnalysis of product designers' creative and cognitive processes. Recommendations for the development of EsQUIsE to support Product Design.
Elsen, Catherine ULiege

Master's dissertation (2007)

The goal of this work is to analyse the product designers’ main habits in creation and cognition, to study the sketches’ contributions to early stages of design processes (these sketches being one common ... [more ▼]

The goal of this work is to analyse the product designers’ main habits in creation and cognition, to study the sketches’ contributions to early stages of design processes (these sketches being one common feature in creation that designers have with architects) in order to evaluate if EsQUIsE could support product design. Several questions sequence this evaluation: i) What are the main product designers’ work’s methods? What type of projects do they usually approach? What kind of issues, constraints do they have to confront? ii) What are the cognitive processes involved during the design phases? iii) When and how do sketches enter these processes? What are their effectiveness, their main contributions and on the opposite their main limitations? iv) Assuming that sketches constitute for product designers an effective support for the early creative phases, what are their principal characteristics? What do they content? What are their predominant graphic codes in this particular domain? v) What are the nowadays Computer-aided design tools’ appointed functions in product design? What are their advantages and drawbacks? How could they become better adapted to preliminary design phases? These interrogations are considered thanks to the following work methodology: • First an information collecting through a short state of art. The following subjects are: projects and constraints analysis; technical background; summary of the principal creative and cognitive processes in design; study of sketches potentials; advantages and limitations of actual CAD tools and short listing of the main software used in product design. • Then a straight contact with designers through interviews in order to collect their points of view on the previous subjects. • Follow the appreciation of the previous theories and collected information thanks to the pragmatic character of two experiments. These experiments bring new elements. They afford a better understanding of the general design theories, they confirm the sketches’ and 3D representations’ contributions to the design processes and furnish the first basis of graphic codes’ and sketches contents’ analysis. • Finally, the observation of graphic designers during the modelling of a simple object permits the deeper analyse of the relationship the user maintains toward nowadays CAD tools. A summary of the multiple aspects EsQUIsE should present to efficiently support the early conception phases in product design concludes this research. [less ▲]

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See detailAnalysis of prolactin receptor expression in the murine brain using a novel prolactin receptor reporter mouse
Kokay, Ilona; Wyatt, Amanda; Phillipps, Hollian et al

in Journal of Neuroendocrinology (2018), 30

Prolactin influences a wide range of physiological functions via actions within the central nervous system, as well as in peripheral tissues. A significant limitation in studies investigating these ... [more ▼]

Prolactin influences a wide range of physiological functions via actions within the central nervous system, as well as in peripheral tissues. A significant limitation in studies investigating these functions is the difficulty in identifying prolactin receptor (Prlr) expression, particularly in the brain. We have developed a novel mouse line using homologous recombination within mouse embryonic stem cells to produce a mouse in which an internal ribosome entry site (IRES) followed by Cre recombinase cDNA is inserted immediately after exon 10 in the Prlr gene, thereby targeting the long isoform of the Prlr. By crossing this Prlr-IRES-Cre mouse with a ROSA26-CAGStauGFP (τGFP) reporter mouse line, and using immunohistochemistry to detect τGFP, we were able to generate a detailed map of the distribution of individual Prlr expressing neurones and fibres throughout the brain of adult mice without the need for amplification of the GFP signal. Because the τGFP is targeted to neurotubules, the labelling detected not only cell bodies, but also processes of prolactin-sensitive neurones. In both males and females, Cre-dependent τGFP expression was localised, with varying degrees of abundance, in a number of brain regions, including the lateral septal nucleus, bed nucleus of the stria terminalis, preoptic and hypothalamic nuclei, medial habenula, posterodorsal medial amygdala, and brainstem regions such as the periaqueductal grey and parabrachial nucleus. The labelling was highly specific, occurring only in cells where we could also detect Prlr mRNA by in situ hybridisation. Apart from two brain areas, the anteroventral periventricular nucleus and the medial preoptic nucleus, the number and distribution of τGFP-immunopositive cells was similar in males and females, suggesting that prolactin may have many equivalent functions in both sexes. These mice provide a valuable tool for investigating the neural circuits underlying the actions of prolactin. [less ▲]

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See detailAnalysis of proline permease gene expression at the molecular level
Jauniaux, J. C.; Vandenbol, Micheline ULiege; Vissers, S. et al

Poster (1986)

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See detailAnalysis of properdin (BF) genotypes associated with litter size in a commercial pig cross population
Buske, Bernd ULiege; Brunsch, Christa; Zeller, Karin et al

in Journal of Animal Breeding and Genetics (2005), 122(4), 259-263

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See detailAnalysis of protamine peptides in insulin pharmaceutical formulations by capillary electrophoresis.
Lamalle, Caroline; Servais, Anne-Catherine ULiege; Demelenne, Alice ULiege et al

in Journal of Separation Science (2016), 39(6), 1189-94

Protamines are a group of highly basic peptides that are sometimes added to insulin formulations to prolong the pharmacological action. In this study, different methods were investigated to identify ... [more ▼]

Protamines are a group of highly basic peptides that are sometimes added to insulin formulations to prolong the pharmacological action. In this study, different methods were investigated to identify protamine in insulin formulations. Capillary electrophoresis in aqueous and non-aqueous media was tested to separate these peptides with very close amino acid sequences. Different buffers (phosphate or formate, both acidified) and various additives (principally negatively charged and neutral surfactants) were investigated to optimize peptide separation. Finally, a micellar electrokinetic capillary chromatography method using a capillary of 120 cm effective length and an aqueous background electrolyte made up of 100 mM phosphate buffer (pH 2) and 50 mM Thesit(R) gave the best results, providing the separation of the four major protamine peptides within 25 min. [less ▲]

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See detailAnalysis of PSII antenna size heterogeneity of Chlamydomonas reinhardtii during state transitions
de Marchin, Thomas ULiege; Ghysels, Bart ULiege; Nicolay, Samuel ULiege et al

in Biochimica et Biophysica Acta. Bioenergetics (2014), 1837(1), 121-130

PSII antenna size heterogeneity has been intensively studied in the past. Based on DCMU fluorescence rise kinetics, multiple types of photosystems with different properties were described. However, due to ... [more ▼]

PSII antenna size heterogeneity has been intensively studied in the past. Based on DCMU fluorescence rise kinetics, multiple types of photosystems with different properties were described. However, due to the complexity of fluorescence signal analysis, multiple questions remain unanswered. The number of different types of PSII is still debated as well as their degree of connectivity. In Chlamydomonas reinhardtii we found that PSIIα possesses a high degree of connectivity and an antenna 2-3 times larger than PSIIβ, as described previously. We also found some connectivity for PSIIβ in contrast with the majority of previous studies. This is in agreement with biochemical studies which describe PSII mega-, super- and core- complexes in Chlamydomonas. In these studies, the smallest unit of PSII in vivo would be a dimer of two core complexes hence allowing connectivity. We discuss the possible relationships between PSIIα and PSIIβ and the PSII mega-, super- and core- complexes. We also showed that strain and medium dependent variations in the half-time of the fluorescence rise can be explained by variations in the proportions of PSIIα and PSIIβ. When analyzing the state transition process in vivo, we found that this process induces an inter-conversion of PSIIα and PSIIβ. During a transition from state 2 to state 1, DCMU fluorescence rise kinetics are satisfactorily fitted by considering two PSII populations with constant kinetic parameters. We discuss our findings about PSII heterogeneity during state transitions in relation with recent results on the remodeling of the pigment-protein PSII architecture during this process. [less ▲]

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See detailAnalysis of PSII antenna size heterogeneity of Chlamydomonas reinhardtii during state transitions - Colloque annuel de la Société Française de Photosynthèse
de Marchin, Thomas ULiege; Ghysels, Bart ULiege; Nicolay, Samuel ULiege et al

Conference (2013, June 18)

PSII antenna size heterogeneity has been extensively studied in the past. Based on in vivo DCMU fluorescence rise kinetics, at least two types of photosystems were described. They differ by their apparent ... [more ▼]

PSII antenna size heterogeneity has been extensively studied in the past. Based on in vivo DCMU fluorescence rise kinetics, at least two types of photosystems were described. They differ by their apparent antenna size and connectivity (this last term refers to the transfer of absorbed energy from a closed PSII unit to an open neighboring unit). In this study, we analysed PSII heterogeneity in Chlamydomonas reinhardtii using non-linear linear regression fitting on in vivo DCMU fluorescence rise kinetics, with a focus on changes in PSII heterogeneity associated with state transitions. We found that PSIIα possesses a high degree of connectivity and an antenna about 3 times larger than PSIIβ, as described previously. In contrast with most earlier studies, we found some connectivity for PSIIβ (although it was highly variable). This is in agreement with recent models based on biochemical and structural analysis of PSII after gel filtration separation which describe PSII mega-, super- and core- complexes in Chlamydomonas. According to these studies, the smallest unit of PSII in vivo would be a dimer of two core complexes hence still allowing connectivity. We also showed that strain and medium dependent variations in the half-time of the fluorescence rise, generally taken as an indicator of the average cross-section of PSII, can be explained by variations in the proportions of PSIIα and PSIIβ. When analyzing the state transition process, we showed for the first time in vivo that it induces an inter-conversion of PSIIα and PSIIβ. These findings are discussed with respect to the latest insights on the remodeling of the pigment-protein PSII architecture during this process. [less ▲]

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See detailAnalysis of quality parameters of whole rapeseed by NIRS
Biston, R.; Dardenne, P.; Cwikowski, M. et al

in Wathelet, Jean-Paul (Ed.) Glucosinolates in Rapeseeds: Analytical Aspects (1987)

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See detailAnalysis of recent pharmaceutical regulatory documents on analytical method validation.
Rozet, Eric ULiege; Ceccato, Attilio ULiege; Hubert, Cédric ULiege et al

in Journal of Chromatography. A (2007), 1158(1-2), 111-25

All analysts face the same situations as method validation is the process of proving that an analytical method is acceptable for its intended purpose. In order to resolve this problem, the analyst refers ... [more ▼]

All analysts face the same situations as method validation is the process of proving that an analytical method is acceptable for its intended purpose. In order to resolve this problem, the analyst refers to regulatory or guidance documents, and therefore the validity of the analytical methods is dependent on the guidance, terminology and methodology, proposed in these documents. It is therefore of prime importance to have clear definitions of the different validation criteria used to assess this validity. It is also necessary to have methodologies in accordance with these definitions and consequently to use statistical methods which are relevant with these definitions, the objective of the validation and the objective of the analytical method. The main purpose of this paper is to outline the inconsistencies between some definitions of the criteria and the experimental procedures proposed to evaluate those criteria in recent documents dedicated to the validation of analytical methods in the pharmaceutical field, together with the risks and problems when trying to cope with contradictory, and sometimes scientifically irrelevant, requirements and definitions. [less ▲]

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See detailAnalysis of recent seizures of synthetic cannabinoids in Liege area
DENOOZ, Raphael ULiege

Scientific conference (2011, October 18)

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See detailAnalysis of regional wall motion during contrast-enhanced dobutamine stress echocardiography: effect of contrast imaging settings.
Cosyns, Bernard; Van Camp, Guy; Droogmans, Sophie et al

in European Journal of Echocardiography (2009), 10(8), 956-60

Aims: Myocardial contrast perfusion echocardiography (MCE) allows simultaneous assessment of perfusion and function. However, low frame rate during MCE may reduce the viewer's ability to discern ... [more ▼]

Aims: Myocardial contrast perfusion echocardiography (MCE) allows simultaneous assessment of perfusion and function. However, low frame rate during MCE may reduce the viewer's ability to discern contractile dysfunction. This study sought to compare MCE and left ventricular opacification (LVO) settings with regard to wall motion abnormalities (WMA) at rest and during dobutamine stress echocardiography (DSE). Methods and results: In 50 patients scheduled for coronary angiography and with poor baseline image quality, MCE and LVO were performed during DSE. Regional wall motion was assessed and inter-observer agreement was determined for each imaging modality. The endocardial border score index was similar for both modalities. The wall motion score index (WMSCI) at peak stress using MCE was well correlated with WMSCI obtained with LVO (r2 = 0.9, P < 0.001). However, WMSCI at peak stress was underestimated by MCE (1.66 ± 0.58 with DSE-LVO vs. 1.535 ± 0.50 with DSE-MCE; P < 0.001). Inter-observer agreement on the presence of WMA was 0.65 for MCE and 0.67 for LVO at peak stress. Conclusion: Myocardial contrast perfusion echocardiography provides equal endocardial border delineation compared with LVO modality. Although the inter-observer agreement is slightly higher with LVO compared with MCE, it is not significantly different with MCE at peak stress. Despite the similar improvement in endocardial border delineation, LVO settings allow the detection of more WMA than MCE at peak stress, leading to a significantly higher accuracy for the detection of ischaemia in patients suspected of coronary artery disease when only wall motion is taken into account. [less ▲]

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See detailAnalysis of risks and impacts on the income of small poultry producers in Hatay Province, North-Vietnam
Phan Dang, Thang ULiege; Vu Dinh, Ton; Duquesne, Brigitte et al

in Hanoi University of Agriculture; Francophone Joint University Council (CIUF) (Eds.) Proceedings of Scientific Research Results - Institutional University Cooperation Program 2008-2012 (2013)

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See detailAnalysis of risks and impacts on the incomes of small poultry producers in Ha Tay Province, North-Vietnam
Phan Dang, Thang; Vu Dinh, Ton; Duquesne, Brigitte ULiege et al

in Sirivaidyapong, Sudson; Wongtavatchai, Janenuj (Eds.) Proceedings of 13th AITVM 2010 International Conference "Globalization of Tropical Animal Diseases and Public Health Concerns", Bankok, Thailand, 23-26 August, 2010 (2010)

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See detailAnalysis of Root Secreted Proteases in Arabidopsis thaliana and Nicotiana tabacum
Désiron, Carole ULiege; De Lemos Esteves, Frédéric ULiege; Natalis, Lucie et al

Poster (2011, June 09)

Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ... [more ▼]

Plants are promising tools to produce complex recombinant proteins like antibodies. When host plants are grown on hydroponics, the production of recombinant proteins that are secreted by the roots ('rhizosecretion') greatly simplifies harvest and purification of the product, during whole plant life. However, proteases represent up to 10% of the naturally secreted proteins and are known to significantly decrease the yield of production by rhizosecretion. In this study, we analyzed the rhizosecreted proteases of Arabidopsis thaliana and Nicotiana tabacum. Total rhizosecreted proteins were recovered by salt extraction and the protease activity was assayed in vitro or by zymography. The relative contribution of major protease families to total activity was evaluated with specific inhibitors and revealed significant differences between the two species. The degradation capacity of the root-secreted proteases was further characterized against selected target proteins: BSA and human IgGs. [less ▲]

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See detailAn analysis of savings among rural poor households in Rwanda
Maniriho, Aristide ULiege; Musabanganji; Lebailly, Philippe

in Das, Gouranga G.; Rukundo, Johnson Bosco (Eds.) Rwandan Economy at the Crossroads of Development - Key Macroeconomic and Microeconomic Perspectives (2020)

A household’s savings are considered a primary source of investments which drive economic growth and development. This study identifies the determinants of private savings among poor rural households in ... [more ▼]

A household’s savings are considered a primary source of investments which drive economic growth and development. This study identifies the determinants of private savings among poor rural households in Rwanda. It uses data from the 5th round of the Integrated Household Living Conditions surveys conducted in Rwanda every 4 years. It specifies a simultaneous equations model and does the estimations using the 2SLS method to account for missing variables and a possible correlation between some covariates and the error term, which is complemented with a T-test and correlation analysis. The results of the T-test show that savings among poor rural households are significantly positive. The econometric estimates show that in contrast with existing literature, income and all other forms of wealth are factors which do not influence the savings of poor rural households, while consumption is positively correlated with savings. We recommend that socioeconomic development programs, projects, and policies that aim at improving income and consumption levels thus affecting savings among poor rural households should be enhanced. [less ▲]

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See detailAn analysis of savings among rural poor households in Rwanda
Maniriho, Aristide ULiege; Musabanganji, Edouard; Lebailly, Philippe ULiege

Conference (2019)

The household’s savings are considered the primary source of the investment, which is in turn the driver of economic growth and development. This study attempted to identify the determinants of private ... [more ▼]

The household’s savings are considered the primary source of the investment, which is in turn the driver of economic growth and development. This study attempted to identify the determinants of private savings among the rural poor households in Rwanda. We used the 5th round of the Integrated Household Living Conditions survey data conducted in Rwanda every four years. A simultaneous-equations model was specified and estimated using the 2SLS method to account for endogeneity and simultaneity bias, complemented with t test and correlation analysis. The results from the t test show that savings among rural poor households is significantly positive. The econometric estimates showed farm income is not among the determinants of saving while consumption is among the most significant drivers of saving of the rural poor in Rwanda. We recommend that socioeconomic development programs, projects and/or policy that aim at improving the level of income and thus affecting the savings among rural poor households should be enhanced. [less ▲]

Detailed reference viewed: 111 (6 ULiège)