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See detailEvidence for a Role of Early Oestrogens in the Central Processing of Sexually Relevant Olfactory Cues in Female Mice
Pierman, S.; Douhard, Quentin ULiege; Bakker, Julie ULiege

in European Journal of Neuroscience (2008), 27(2), 423-31

We previously found that female aromatase knockout (ArKO) mice showed less investigation of socially relevant odours as well as reduced sexual behaviour. We now ask whether these behavioural deficits ... [more ▼]

We previously found that female aromatase knockout (ArKO) mice showed less investigation of socially relevant odours as well as reduced sexual behaviour. We now ask whether these behavioural deficits might be due to an inadequate processing of odours in female ArKO mice. Therefore, we exposed female ArKO mice to same- and opposite-sex urinary odours and determined the expression of the immediate early gene c-Fos along the main and accessory olfactory projection pathways. We included ArKO males in the present study as we previously observed that they show female-typical detection thresholds of urinary odours, suggesting a role for perinatal oestrogens in these behavioural responses. No sex or genotype differences were observed in the olfactory bulb after urine exposure. By contrast, sex differences in c-Fos responses were observed in wild-type (WT) mice following exposure to male urine in the more central regions of the olfactory pathway; only WT females showed a significant Fos induction in the amygdala, central medial pre-optic area and ventromedial hypothalamus. However, ArKO females did not show a c-Fos response to male odours in the ventromedial hypothalamus, suggesting that the processing of male odours is affected in ArKO females and thus that oestrogens may be necessary for the development of neural responses to sexually relevant odours in female mice. By contrast, c-Fos responses to either male or oestrous female urine were very similar between ArKO and WT males, pointing to a central role of androgen vs. oestrogen signalling in the male circuits that control olfactory investigation and preferences. [less ▲]

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See detailEvidence for a role of heat shock factor 1 in inhibition of NF-kB pathway during heat shock response-mediated lung protection
Wirth, D.; Bureau, Fabrice ULiege; Melotte, D. et al

in American Journal of Physiology - Lung Cellular and Molecular Physiology (2004), 287

Heat shock transcription factor (HSF)-1 is recognized as a central component of the heat shock response, which protects against various harmful conditions. However, the mechanisms underlying the ... [more ▼]

Heat shock transcription factor (HSF)-1 is recognized as a central component of the heat shock response, which protects against various harmful conditions. However, the mechanisms underlying the protection and the role of HSF-1 in these mechanisms have not yet been clearly elucidated. Using HSF-1 knockout mice (Hsf1_/_), we examined whether heat shock responsemediated lung protection involved an inhibition of the proinflammatory pathway via an interaction between HSF-1 and NF-_B, in response to cadmium insult. The HSF-1-dependent protective effect against intranasal instillation of cadmium (10 and 100 _g/mouse) was demonstrated by the higher protein content (1.2- and 1.4-fold), macrophage (1.6- and 1.9-fold), and neutrophil (2.6- and 1.8-fold) number in bronchoalveolar fluids, higher lung wet-to-dry weight ratio, and more severe lung damage evaluated by histopathology in Hsf1_/_compared with wild-type animals. These responses were associated with higher granulocyte/macrophage colony-stimulating factor (GMCSF; 1.7-fold) but not TNF-_ concentrations in bronchoalveolar fluids of Hsf1_/_ mice compared with those of wild-type animals, indicating that HSF-1 behaved as a repressor of specific cytokine production in our model. To further investigate the mechanism of GM-CSF repression, we analyzed the NF-_B activity and I_B stability. The DNA binding NF-_B activity, in particular p50 homodimer activity, was higher in Hsf1_/_ mice than in wild-type mice after cadmium exposure. These results provide a first line of evidence that mechanisms of lung protection depending on HSF-1 involve specific cytokine repression via inhibition of NF-_B activation in vivo. [less ▲]

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See detailEvidence for a role of microRNA-21 and microRNA-125b in negatively regulating angiogenic processes
Malvaux, Ludovic ULiege

Doctoral thesis (2011)

Recently discovered, miRNAs have quickly become strong regulators of biological processes. These small non-coding RNAs of about 22 nucleotides partially base pair to the 3’UTR of the targeted mRNAs and ... [more ▼]

Recently discovered, miRNAs have quickly become strong regulators of biological processes. These small non-coding RNAs of about 22 nucleotides partially base pair to the 3’UTR of the targeted mRNAs and repress them. Due to their wide range effects, microRNAs were extensively studied in various diseases and were rapidly demonstrated to be deregulated in pathologies such as cancer. More recently, they have been shown to be implicated in vascular network formation (angiogenesis) and were proposed to be used in anti-angiogenic therapies. Nowadays about twenty angiomiRs have been discovered including the endothelial specific miR-126. As observed in several miRNA profiling of endothelial cells and confirmed in our laboratory in HUVECs (human umbilical veins endothelial cells), miR-21 and miR- 125b are highly expressed in this cell type suggesting that these miRNAs could play a role in vascular network formation. We then studied the implication of miR-21 and miR-125b in in vitro as well as in vivo angiogenesis. One of the most studied miRNA in cancer progression is miR-21 as it was shown to modify proliferating properties of numerous tumor cells. Our experiments revealed that miR-21 overexpression and inhibition have no direct effect on endothelial cells proliferation rate. However, miR-21 overexpression leads to the inhibition of HUVECs migration and tube formation as demonstrated in in vitro angiogenic assays. Moreover, opposite effects were observed upon miR-21 inhibition. We also confirmed that RhoB, a small Rho-GTPase implicated in stress fibers formation, is involved in these phenomena as RhoB inhibition using siRNA mimics miR-21 overexpression in endothelial cells. Moreover, miR-21 modulation affects RhoB mRNA and protein expressions. We further demonstrated a direct interaction between miR-21 and the RhoB 3’UTR confirming that miR-21 modulates angiogenesis partially through its effect on RhoB expression. A similar approach was used to study the implication of miR-125b in vascular network formation. In vivo, miR-125b expression was modulated in the zebrafish revealing that miR-125b expression needs to be controlled for proper intersomitic blood vessels establishment. In vitro, miR-125b overexpression decreases HUVECs migration and tube formation whereas miR-125b inhibition increases these functions. A transcriptomic analysis suggests that numerous adhesion molecules such as VE-cadherin or MCAM are involved in these processes. Furthermore, other proteins known to regulate angiogenesis such as the transcription factor ETS1 and the VEGFA receptor, VEGFR2 were also shown to be regulated by miR-125b. This observation confirms that miR-125b modulates angiogenic properties of endothelial cells. Finally, we investigated the impact of miR-21 and miR-125b overexpression in an in vivo pathological model of angiogenesis. In a mouse model of choroïdal neovascularization we demonstrated that miR-21 or miR-125b overexpression in the eyes of these mice decreases blood vessel establishment suggesting that these microRNAs could be used as therapeutic antiangiogenic agents. Taken together, the results presented in this thesis show that miR-21 and miR-125b regulate angiogenesis in vitro and in vivo. [less ▲]

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See detailEvidence for a role of prolactin fragments in corpus luteum function
Ricken, A.; Merkwitz, C.; Struman, Ingrid ULiege et al

in The society for the study of reproduction Annual meeeting, Quebec 2005 (2005)

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See detailEvidence for a role of sleep in forgetting of irrelevant information
Collette, Fabienne ULiege; Rauchs, Géraldine; Landeau, Brigitte et al

in NeuroImage (2009, June), 47(Suppl 1), 328-

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See detailEvidence for a role of the Simian Virus 40 in human breast carcinomas
Hachana, Mohamed Ridha ULiege; Trimeche, Mounir; Ziadi, Sonia et al

in Breast Cancer Research and Treatment (2009), 113(1), 43-58

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See detailEvidence for a Second Receptor Binding Site on Human Prolactin
Goffin, Vincent; Struman, Ingrid ULiege; Mainfroid, V. et al

in Journal of Biological Chemistry (1994), 269(51), 32598-606

The existence of a second receptor binding site on human prolactin (hPRL) was investigated by site-directed mutagenesis. First, 12 residues of helices 1 and 3 were mutated to alanine. Since none of the ... [more ▼]

The existence of a second receptor binding site on human prolactin (hPRL) was investigated by site-directed mutagenesis. First, 12 residues of helices 1 and 3 were mutated to alanine. Since none of the resulting mutants exhibit reduced bioactivity in the Nb2 cell proliferation bioassay, the mutated residues do not appear to be functionally necessary. Next, small residues surrounding the helix 1-helix 3 interface were replaced with Arg and/or Trp, the aim being to sterically hinder the second binding site. Several of these mutants exhibit only weak agonistic properties, supporting our hypothesis that the channel between helices 1 and 3 is involved in a second receptor binding site. We then analyzed the antagonistic and self-antagonistic properties of native hPRL and of several hPRLs analogs altered at binding site 1 or 2. Even at high concentrations (approximately 10 microM), no self-inhibition was observed with native hPRL; site 2 hPRL mutants self-antagonized while site 1 mutants did not. From these data, we propose a model of hPRL-PRL receptor interaction which slightly differs from that proposed earlier for the homologous human growth hormone (hGH) (Fuh, G., Cunningham, B. C., Fukunaga, R., Nagata, S., and Goeddel, D. V., and Well, J. A. (1992) Science 256, 1677-1680). Like hGH, hPRL would bind sequentially to two receptor molecules, first through site 1, then through site 2, but we would expect the two sites of hPRL to display, unlike the two binding sites of hGH, about the same binding affinity, thus preventing self-antagonism at high concentrations. [less ▲]

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See detailEvidence For a Self-Enrichment Process in Galactic Halo Globular Clusters
Parmentier, G.; Jehin, Emmanuel ULiege; Magain, Pierre ULiege et al

in Grebel, E.; Bradner, W. (Eds.) Modes of Star Formation and the Origin of Field Populations (2002)

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See detailEvidence for a sharp structure variation inside a red-giant star
Miglio, Andrea ULiege; Montalban Iglesias, Josefa ULiege; Carrier, F. et al

in Astronomy and Astrophysics (2010), 520

Context. The availability of precisely determined frequencies of radial and non-radial oscillation modes in red giants is finally paving the way for detailed studies of the internal structure of these ... [more ▼]

Context. The availability of precisely determined frequencies of radial and non-radial oscillation modes in red giants is finally paving the way for detailed studies of the internal structure of these stars. <BR /> Aims: We look for the seismic signature of regions of sharp structure variation in the internal structure of the CoRoT target HR 7349. <BR /> Methods: We analyse the frequency dependence of the large frequency separation and second frequency differences, as well as the behaviour of the large frequency separation obtained with the envelope auto-correlation function. <BR /> Results: We find evidence for a periodic component in the oscillation frequencies, i.e. the seismic signature of a sharp structure variation in HR 7349. In a comparison with stellar models we interpret this feature as caused by a local depression of the sound speed that occurs in the helium second-ionization region. Using solely seismic constraints this allows us to estimate the mass (M = 1.2[SUB]-0.4[/SUB][SUP]+0.6[/SUP] M_&sun;) and radius (R = 12.2[SUB]-1.8[/SUB][SUP]+2.1[/SUP] R_&sun;) of HR 7349, which agrees with the location of the star in an HR diagram. [less ▲]

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See detailEvidence for a specific impairment of serial order short-term memory in dyslexic children
Martinez Perez, Trecy ULiege; Majerus, Steve ULiege; Mahot, Aline et al

in Dyslexia : The Journal of the British Dyslexia Association (2012), 18(2), 94-109

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See detailEvidence for an own-age bias in age estimation from voices in older persons
Moyse, Evelyne ULiege; Beaufort, Aline ULiege; Brédart, Serge ULiege

in European Journal of Ageing (2014)

Previous studies have investigated the effect of ageing on age estimation from faces as well as the occur- rence of an own-age bias in such age estimation from faces. To the best of our knowledge, the ... [more ▼]

Previous studies have investigated the effect of ageing on age estimation from faces as well as the occur- rence of an own-age bias in such age estimation from faces. To the best of our knowledge, the occurrence of an own age effect on age estimation from voices has never been examined earlier using an experimental design in which the age of participants (young vs. old) and the age of voice stimuli (young vs. old) were crossed. Results revealed an own-age bias in older adults only. In comparison with younger adults, older participants showed age estimation abilities that are preserved for voices from their own age group and impaired for younger voices. This own age bias was absent in younger participants. [less ▲]

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See detailEvidence for association between the HLA-DQA locus and abdominal aortic aneurysms in the Belgian population: a case control study.
Ogata, Toru; Gregoire, Lucie; Goddard, Katrina A B et al

in BMC Medical Genetics (2006), 7

BACKGROUND: Chronic inflammation and autoimmunity likely contribute to the pathogenesis of abdominal aortic aneurysms (AAAs). The aim of this study was to investigate the role of autoimmunity in the ... [more ▼]

BACKGROUND: Chronic inflammation and autoimmunity likely contribute to the pathogenesis of abdominal aortic aneurysms (AAAs). The aim of this study was to investigate the role of autoimmunity in the etiology of AAAs using a genetic association study approach with HLA polymorphisms. METHODS: HLA-DQA1, -DQB1, -DRB1 and -DRB3-5 alleles were determined in 387 AAA cases (180 Belgian and 207 Canadian) and 426 controls (269 Belgian and 157 Canadian) by a PCR and single-strand oligonucleotide probe hybridization assay. RESULTS: We observed a potential association with the HLA-DQA1 locus among Belgian males (empirical p = 0.027, asymptotic p = 0.071). Specifically, there was a significant difference in the HLA-DQA1*0102 allele frequencies between AAA cases (67/322 alleles, 20.8%) and controls (44/356 alleles, 12.4%) in Belgian males (empirical p = 0.019, asymptotic p = 0.003). In haplotype analyses, marginally significant association was found between AAA and haplotype HLA-DQA1-DRB1 (p = 0.049 with global score statistics and p = 0.002 with haplotype-specific score statistics). CONCLUSION: This study showed potential evidence that the HLA-DQA1 locus harbors a genetic risk factor for AAAs suggesting that autoimmunity plays a role in the pathogenesis of AAAs. [less ▲]

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See detailEvidence for atypical categorical speech perception in Williams syndrome.
Majerus, Steve ULiege; Poncelet, Martine ULiege; Serniclaes, W. et al

Conference (2010, May 27)

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See detailEvidence for atypical categorical speech perception in Williams syndrome.
Majerus, Steve ULiege; Poncelet, Martine ULiege; Bérault, Aurélie et al

in Journal of Neurolinguistics (2011), 24

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See detailEvidence for Auroral Emissions from Callisto's Footprint in HST UV Images
Bhattacharyya, Dolon; Clarke, John T.; Montgomery, Jordan et al

in Journal of Geophysical Research. Space Physics (in press)

Auroral emissions are expected from the footprint of Callisto in Jupiter's upper atmosphere owing to the known interaction of its atmosphere with Jupiter's magnetosphere, and from the observed auroral ... [more ▼]

Auroral emissions are expected from the footprint of Callisto in Jupiter's upper atmosphere owing to the known interaction of its atmosphere with Jupiter's magnetosphere, and from the observed auroral emissions from the footprints of the other three Galilean satellites. The mapping of Callisto along modeled magnetic field lines at Jupiter, however, places the expected footprint at the same latitude as the main auroral emissions, making it difficult to detect. We analyzed ultraviolet images of Jupiter taken using the HST/ACS instrument during a large observing campaign in 2007. Using a co-addition method similar to one used for Enceladus, we have identified a strong candidate for the footprint of Callisto on May 24, 2007. We tested this finding by applying the same co-addition technique to a nearly identical auroral configuration on May 30, 2007 when Callisto was behind Jupiter, not visible from Earth (CML = 22°; sub-Callisto system III longitude = 327°). By comparing the two co-added images, we can clearly see the presence of a strongly sub-corotating spot close to the expected Callisto footprint location on 24th May and its absence on 30th May. On the 24th Callisto was located in the current sheet. We also found a probable candidate on 26th May 2007 during which time Callisto was positioned below the current sheet. The measured location and intensity of the auroral emission provides important information about the interaction of Callisto with Jupiter's magnetic field, the corotating plasma, and the neutral and ionized state of the thin atmosphere of Callisto. [less ▲]

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See detailEvidence for basement membranes in rat tail tendon sheaths.
Guizzardi, S.; Foidart, Jean-Michel ULiege; Leonardi, L. et al

in Basic and Applied Histochemistry (1987), 31(2), 177-81

The presence of anti-laminin antibodies and a basement membrane-like thin electrondense lamina has been demonstrated in the peritendineum of the rat tail tendon by indirect immunofluorescence and electron ... [more ▼]

The presence of anti-laminin antibodies and a basement membrane-like thin electrondense lamina has been demonstrated in the peritendineum of the rat tail tendon by indirect immunofluorescence and electron microscopy. [less ▲]

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See detailEvidence for behavioral sensitization to cocaine in preweanling rat pups
Wood, R. D.; Tirelli, Ezio ULiege; Snyder, K. J. et al

in Psychopharmacologia (1998), 138(2), 114-123

Sought to determine whether promoting context-dependent sensitization might facilitate expression of sensitization in preweanlings. Sprague-Dawley rats were injected daily from postnatal day 14 to ... [more ▼]

Sought to determine whether promoting context-dependent sensitization might facilitate expression of sensitization in preweanlings. Sprague-Dawley rats were injected daily from postnatal day 14 to postnatal day 20 with 0, 5, 15, or 30 mg/kg cocaine hydrochloride and placed for 30 min in either the experimental chamber or home cage. On postnatal day 21 (test day), Ss were challenged with either 15 mg/kg cocaine or saline prior to placement in the experimental chamber. Significant sensitization of cocaine-induced stereotyped head movements was evident in animals given 15 or 30 mg/kg chronically in the experimental chamber, but not when these same doses were given in the home cage. Less consistent evidence for cocaine-induced sensitization was seen when examining locomotion, although trends for sensitization of this behavior were seen in animals chronically injected in either the test chamber or home cage. Thus, preweanlings can exhibit cocaine sensitization, particularly in terms of stereotypy, when tested shortly after the chronic exposure period, with expression of this sensitization being facilitated by pairing the chronic injections with the test context. ((c) 1998 APA/PsycINFO, all rights reserved) [less ▲]

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See detailEvidence for cooperative effects in the exchange reaction catalysed by the oxoglutarate translocator of rat-heart mitochondria.
Sluse, Francis ULiege; Sluse-Goffart, Claudine; Duyckaerts, Claire ULiege et al

in European Journal of Biochemistry (1975), 56(1), 1-14

The initial rates of the exchange external oxoglutarate/internal malate through the inner membrane of rat-heart mitochondria, for various concentrations of the two substrates, have been reinvestigated for ... [more ▼]

The initial rates of the exchange external oxoglutarate/internal malate through the inner membrane of rat-heart mitochondria, for various concentrations of the two substrates, have been reinvestigated for an extended range of concentrations of the external oxoglutarate. This has been made possible by use of the inhibitor-stop technique that allows 100 times smaller incubation times than the centrifugation-stop technique used previously. Under the experimental conditions the uptake of the external-labelled oxoglutarate into the mitochondrial-matrix space is mediated by the oxoglutarate translocator performing a ono-to-one exchange of the anions oxoglutarate (external) and malate (internal). Two intermediary-plateau regions are observed in the kinetic saturation curve of the translocator by the external oxoglutarate, revealing a complex rate equation which is found to be the product of two one-substrate functions. Analysing these features it is shown that the model, proposed earlier, of a "double carrier" as catalyst in a rapid-equilibrium random bi-bi mechanism, is still applicable but that several external binding sites have to be considered. As already noticed the external and the internal substrates bind to their respective sites independently of each other. Furthermore, some additional requirements imposed by the observed kinetics suggest that the exchange reaction is performed by only one translocator species made of identical interacting subunits. The anion exchange is tentatively viewed as a rotation of a subunit around an axis situated in the plane of the membrane after two independent local configuration changes induced by the binding of the two substrates on this subunit. [less ▲]

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See detailEvidence for cross-talk between the LH receptor and LH during implantation in mice
Gridelet, Virginie ULiege; Tsampalas, Marie; Berndt, Sarah et al

in Reproduction, Fertility and Development (2013), 25

The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model ... [more ▼]

The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst. The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription–polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation. [less ▲]

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