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See detailStudy of insect diversity in vegetable crops in Libreville and Owendo (Gabon)
Bayendi-Loudit, Sandrine ULiege; Francis, Frédéric ULiege; Ndoutoume Ndong, Auguste et al

Poster (2014, December 12)

Gabon is a Central African country rich in diversity of plant and animal species. Vegetable production increases every year, this development causes the proliferation of pests and pathogens. In 2012 ... [more ▼]

Gabon is a Central African country rich in diversity of plant and animal species. Vegetable production increases every year, this development causes the proliferation of pests and pathogens. In 2012, study was conducted in three market garden perimeters in Libreville and Owendo to ensure a sustainable pest control. Records of yellow traps and weekly visual observations were made in plots of pigweed (Amaranthus sp. Amaranthaceae), of red sorrel (Hibiscus sabdariffa L, Malavaceae), of tomatoes (Lycopersicon esculentum Mill, Solanaceae) and of cabbage (Brassica oleracea L., Brassicaceae). A total of 7119 insects were counted and taxonomically classified into 8 separate orders including 81 families. The main pests were represented by 4 families: Aphididae (31.0%), Cecidomyiidae (5.1%), Chrysomelidae (4.0%), Thripidae (2.0%) and 6 families of useful insects (pollinators and predators): Dolichopodidae (5.4%), Andrenidae (2.6%), Coccinellidae (1.7%), Syrphidae (0.6%), Braconidae (0.5%), Hemerobiidae (0.1%). Entomological diversity is discussed according to the local conditions. [less ▲]

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See detailMeasuring the Effect of Stochastic Perturbation Component in Cellular Automata Urban Growth Model
El Saeid Mustafa, Ahmed Mohamed ULiege; Saadi, Ismaïl ULiege; Cools, Mario ULiege et al

in Procedia Environmental Sciences (2014), 22(Winter), 156168

Urban environments are complex dynamic systems whose prediction of the future states cannot exclusively rely on deterministic rules. Although several studies on urban growth were carried out using ... [more ▼]

Urban environments are complex dynamic systems whose prediction of the future states cannot exclusively rely on deterministic rules. Although several studies on urban growth were carried out using different modelling approaches, the measurement of uncertainties was commonly neglected in these studies. This paper investigates the effect of uncertainty in urban growth models by introducing a stochastic perturbation method. A cellular automaton is used to simulate predicted urban growth. The effect of stochastic perturbation is addressed by comparing series of urban growth simulations based on different degree of stochastic perturbation randomness with the original urban growth simulation, obtained with the sole cellular automata neighbouring effects. These simulations are evaluated using cell-to-cell location agreement and a number of spatial metrics. The model framework has been applied to the Ourthe river basin in Belgium. The results show that the accuracy of the model is increased by introducing a stochastic perturbation component with a limited degree of randomness, in the cellular automata urban growth model. [less ▲]

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See detailAnalphabétisme secondaire, raison cynique et la culture pour tous.
Hamers, Jérémy ULiege

Conference (2014, December 12)

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See detailThe cerato-mandibular ligament: an innovation for sound production and feeding in damselfishes (Pomacentridae).
Olivier, Damien ULiege; Frederich, Bruno ULiege; Spanopoulos-Zarco, Milton et al

Conference (2014, December 12)

The success of a taxonomic group can be promoted by a key character that allows the group to interact with its environment in a different way and to potentially occupy new niches. The Pomacentridae ... [more ▼]

The success of a taxonomic group can be promoted by a key character that allows the group to interact with its environment in a different way and to potentially occupy new niches. The Pomacentridae possess a synapomorphic trait, the cerato-mandibular (c-md) ligament, which joins the hyoid bar to the inner part of the lower jaw. It has previously been shown that this ligament is a key trait in communication in damselfishes because it enables them to slam the oral jaws shut causing teeth collision and sound production. This specific behavior of mouth closing could, however, also be used for other tasks, such as feeding. Many territorial damselfishes are referred to as farmers, due to their ability to manage algal crops on which they feed. This study hypothesizes that the c-md ligament provides an advantage for grazing filamentous algae, and should thus be considered a key trait for farming behavior. The kinematic patterns associated with sound production and biting filamentous algae or attached animal prey are all based on the same mechanism and are associated with a slam of the oral jaws. We observed that transection of the c-md ligaments makes the fish unable to perform such actions. This study shows that the c-md ligament is a key trait both for sound production and for grazing activities in damselfishes. The buccal jaw slam enables the fish to perform accurate strikes on small filamentous algae. This kind of bite probably plays a major role in farming activity and allows grazing damselfishes to occupy distinct niches, possibly increasing their competitive evolutionary success. [less ▲]

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See detailColonization of a new habitat by copepods: An in situ experiment
Mascart, Thibaud ULiege; Lepoint, Gilles ULiege; Biondo, Renzo ULiege et al

Conference (2014, December 12)

Colonization of new habitats by a biological community is conspicuous and this dynamic process is one of the architectural forces of the biogeographical distribution we know today. Within the meiofauna ... [more ▼]

Colonization of new habitats by a biological community is conspicuous and this dynamic process is one of the architectural forces of the biogeographical distribution we know today. Within the meiofauna (<1mm), copepods (Crustacea) have successfully adapted to nearly every ecosystem and heir colonization power of permanent habitats is therefore well-established. However, few studies tackled the colonization of new naturally occurring provisional habitats, which are of ecological interest since they are rich in organic material, structurally complex and devoted of native fauna. Hence, the present study investigated the copepod colonization of provisional macrophytodetritus (mainly composed of senescent leaves and drift macroalgae) accumulated on bare sand patches inside a Mediterranean Posidonia oceanica seagrass meadow. General motive of colonization such as food and shelter are well-defined. However, little is known regarding the mode of the colonization and source pool of the associated colonists. Here, an in situ experiment was deployed in order to understand the mode of copepod’s colonization to fauna deprived macrophytodetritus. The objectives were: (1) assessing the adjacent colonist’s source pool (i.e. sediment, water column or P. oceanica canopy), (2) investigating the speed of settlement and (3) quantifying the species composition of the colonizing copepods. In summary: (1) species from every source pool actively colonized the macrophytodetritus through the water column and through the sediment-macrophytodetritus interface. (2) The initial settlement occurred within the first 24 hours. (3) The species composition showed to be different than the source’s composition. After 24h, the composition was similar to 45% of the P. oceanica, 28% of the water column and 25% of the sediments. After 96h, the composition was similar to 24% of the P. oceanica, 13% of the water column and 10% of the sediments. Indicating an evolution towards a macrophytodetritus copepod specific community composed of a mixture of the adjacent habitats first colonizers. [less ▲]

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See detailSeasonal sampling and stable isotopes use to delineate seagrass phytodetritus macrofauna trophic ecology: baseline variation or actual diet change?
Remy, François ULiege; Mascart, Thibaud ULiege; Dauby, Patrick ULiege et al

Conference (2014, December 12)

In Mediterranean exported seagrass macrophytodetritus accumulations, a diverse (more than 130 species) and abundant (up to 4900 id.m-2) macrofauna assemblage is found alongside meiofauna, microalgae ... [more ▼]

In Mediterranean exported seagrass macrophytodetritus accumulations, a diverse (more than 130 species) and abundant (up to 4900 id.m-2) macrofauna assemblage is found alongside meiofauna, microalgae, fungi and bacteria. Macrophytodetritus are mainly composed of poorly digestible yet highly colonized material: the dead leaves of the very productive (300 to 2000 g dry wt m-2 yr-1) endemic seagrass Posidonia oceanica. A key role may be played by macrofauna, and more particularly by litter vagile macroinvertebrates (invertebrates > 500µm), in the degradation, enrichment and carbon transfer from P. oceanica to coastal food webs. Indeed, results of gut content observations of the most abundant species show that even if only a few of these species ingest a large proportion of P.oceanica dead leaves fragments, most of the others ingest a small but non-negligible part, suggesting a potential role of the whole community in the mechanical fragmentation of the dead leaves. Mediterranean exported macrophytodetritus accumulations are very dynamic habitats with very variable food availability, quality, and composition. Such an inconstant habitat may result in drastic modifications of the invertebrate community but also of its trophic structure and ecology. To test this hypothesis of influence of pulsed availability, quality and composition of food sources on the vagile macrofauna diet, we took seasonal samples in Calvi Bay (Corsica, 8°45’E; 42°35’N), at two sites between August 2011 and May 2012. Gut content observations and C/N/S stable isotope analysis of bulk tissues were conducted on both the macrofauna and their potential food sources. Significant seasonal and spatial differences of ingestion patterns of the most abundant species were emphasised as were differences of isotopic signatures. “SIAR” Bayesian mixing model and “SIBER” package were used to analyse isotopic data and determine if these differences were due to actual diet modifications or only to baselines isotopic composition variations. [less ▲]

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See detailImpact of Climate Change on “Aphid - Natural Enemies” Relationship
Boullis, Antoine ULiege; Oostrom, Marjolein; Francis, Frédéric ULiege et al

Poster (2014, December 12)

While the effects of global atmospheric changes on vegetation and associated insect populations (bottom-up interactions) are increasingly studied, how these gases affect the interactions between insects ... [more ▼]

While the effects of global atmospheric changes on vegetation and associated insect populations (bottom-up interactions) are increasingly studied, how these gases affect the interactions between insects and their natural enemies (top-down interactions) is less clear. As the efficacy of natural enemies is governed largely by behavioral mechanisms, changes in their prey-seeking behavior or the behavior of insect prey defenses can change the dynamics of insect populations. The impact of increases in atmospheric carbon dioxide concentrations on aphid population dynamic is well documented. However, nothing about their chemical ecology is reported in the literature. Aphids are using many chemical signals to communicate with each other or with their environment. For example aphids produce an alarm pheromone to signal the presence of a natural enemy (such as a predator or a parasitoid) in the colony. Moreover, this pheromone is used by natural enemies as a kairomone to locate aphid prey, and is thus at the center of aphid – natural enemies interactions. In this study, the impact of elevated CO2 concentration on the emission of the alarm pheromone in the aphid A. pisum is studied. Using a zNoseTM (Gas chromatograph enabling the fast analyze of the chemical composition of a sample), the kinetic of the EBF emission in real-time is set up for a single individual predated by a coccinellid predator Harmonia axyridis Pallas. This experimentation is done both for aphids reared under ambient atmospheric conditions and for individuals reared under elevated CO2 concentrations. We present the differences in terms of emission dynamic and discuss the potential of these results in terms of biological control. [less ▲]

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See detailDetection of small amounts of human adenoviruses in stools: comparison of a new immuno real-time PCR assay with classical tools.
Bonot, Sebastian; Ogorzaly, Leslie; Elmoualij, Benaïssa ULiege et al

in Clinical Microbiology and Infection (2014), 20(12), 1-7

The detection of low virus concentrations in biological matrices, especially stool samples, is facing significant limitations as far as common diagnostic methods (enzyme-linked-immunosorbent assay (ELISA ... [more ▼]

The detection of low virus concentrations in biological matrices, especially stool samples, is facing significant limitations as far as common diagnostic methods (enzyme-linked-immunosorbent assay (ELISA) or quantitative real-time PCR (qPCR)) are considered. Here the development of a new immuno real-time PCR (iPCR) is described and its performance in the detection of human adenoviruses (HAdVs) in spiked stools is compared with those of ELISA and qPCR assays. For the iPCR, detection of the sandwich formed by the complexation of capture antibody-antigen-detection antibody was performed by qPCR thanks to the substitution of peroxydase by a chimeric DNA. This modification increased the detection sensitivity 200-fold compared to ELISA. The direct qPCR results revealed that only 0.3–9.5% of the spiked HAdV were detectable, resulting from important losses of DNA occurring at the extraction step. This step was not necessary in the iPCR workflow, avoiding this drawback. The losses of viral particles occurred at the elution step from the stool only. The recovery rate of the iPCR was thus better and ranged between 21 and 54%. As a result, iPCR enabled the detection of lower virus concentrations in stool samples compared to those detected by ELISA and qPCR. The iPCR could be considered as a ‘hyper sensitive ELISA’ for early detection of HAdV infections, especially in the case of immunocompromised patients after haematopoietic stem cell transplant. [less ▲]

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See detailGenetic structure of fragmented southern populations of African Cape Buffalo (Syncerus caffer caffer) based on microsatellite analysis
Smitz, Nathalie ULiege; Cornélis, Daniel; Chardonnet, Philippe et al

Poster (2014, December 12)

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See detailMeurtres de dragons, entre récits mythiques et séquences liturgiques
Swennen, Philippe ULiege

Scientific conference (2014, December 12)

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See detailSpatial variation in the concentrations of mercury and persistent organic pollutants in free-ranging bottlenose dolphins (Tursiops truncatus) from South Florida
Damseaux, France ULiege; Kiszka, Jeremy; Heithaus et al

Poster (2014, December 12)

The bottlenose dolphin (Tursiops truncatus) is an abundant apex predator found in nearshore waters of South Florida, especially in the Lower Florida Keys (Key West) and the coastal waters of Everglades ... [more ▼]

The bottlenose dolphin (Tursiops truncatus) is an abundant apex predator found in nearshore waters of South Florida, especially in the Lower Florida Keys (Key West) and the coastal waters of Everglades National Park (ENP). The objective of this study was to assess variation in contamination levels of total mercury (T-Hg) and persistent organic pollutants (NDL-PCBs, PBDEs, DDT, HCH, HCB, PCDD/Fs and DL-PCBs) in bottlenose dolphins found offshore of the densely populated Key West (n = 27) and from undeveloped ENP (n = 20). T-Hg and POPs were analysed in skin and blubber, respectively, by the mean of Direct Mercury analyser (for THg), GC-ECD (POPs) and GC-HRMS (DLCs). The 7 ICES PCBs were the main compounds found in bottlenose dolphins from Key West (8229 ng.g-1 lipids) and the ENP (2289 ng.g-1 lipids), while the concentrations of PCDD/Fs remained low (Key West: 104 pg.g-1 lipids, ENP: 102 ng.g-1 lipids). POP concentrations were higher in individuals from Key West compared to those from the ENP. However, POPs concentrations in Key West dolphins were lower than those from other locations in Florida and around the world. Unlike organic pollutants, T-Hg concentrations were significantly higher in ENP dolphins (Key West: 2941 ng.g-1 dw versus ENP: 9314 ng.g-1 dw) highlighting the specific cycle of Hg in mangrove ecosystems. To conclude, sources of T-Hg and POPs differed between Key West and ENP as reflected by their concentrations in skin and blubber of free-ranging bottlenose dolphins highlighting their role as sentinels of their environment. [less ▲]

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See detailPolyplex based on polycarbonate polymers for an efficient delivery of HDAC5 and HDAC7 siRNA
Frère, Antoine ULiege; Evrard, Brigitte ULiege; Mespouille, Laetitia et al

Conference (2014, December 12)

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See detailParticulate soils adherence and surface cleanability: influence of biomacromolecules at interfaces and of substrate hydrophobicity
Toure, Yetioman ULiege

Doctoral thesis (2014)

Cleaning of particulate soils is an important issue in food and pharmaceutical production. Understanding the adherence of these soils is a fundamental requirement for improving surfaces cleanability. The ... [more ▼]

Cleaning of particulate soils is an important issue in food and pharmaceutical production. Understanding the adherence of these soils is a fundamental requirement for improving surfaces cleanability. The combination of particles and macromolecules substances appears in many processes. Adsorbed compounds from these mixtures may influence interactions at interfaces and thus fouling and cleaning. This thesis deals with a deeper understanding of the physico-chemical mechanisms affecting soiling and cleanability of open substrates in the presence of biomacromolecules (dextran, bovine serum albumin – BSA, beta-lactoglobulin – β-LGB). Model substrates were chosen according to their hydrophobicity. The influence of macromolecules was examined by introducing them in the quartz suspension taken as a model of hard hydrophilic soil used for soiling, or by conditioning the substrate prior to soiling. The substrates were pretreated with ethanol (-Eth.), piranha or UV-Ozone (-UVO); soiled by spraying the quartz suspensions, then dried, before cleaning assessment. The removal of the soiling particles was evaluated after exposure to water in a radial flow chamber (RFC). Auxiliary characterizations were surface tension and contact angle measurements, surface analysis of the substrate by X-ray photoelectron spectroscopy (XPS) and Scanning electron microscopy (SEM). The interpretation of XPS data allowed the complexity due to the ubiquitous presence of organic contaminants to be coped with, and the surface composition to be expressed in terms of both the amount of adlayer and the mass concentration of adlayer constituents. The contact of substrates with proteins led to their adsorption, which dominated the composition of the organic layer with respect to contaminants initially present, and was not markedly desorbed upon rinsing. Dextran was easily removed in presence of water, independently on the substrate nature. Surface hydrophobicity was shown to influence the morphology of the aggregates resulting from drying. The rounder aggregates formed on polystyrene when soiling was performed with suspension in pure water are more sensitive to wall shear stress than flatter ones formed on more hydrophilic substrate. This is the result of the competing processes of droplet rolling and coalescing, on the one hand, and droplet spreading, on the other hand. It affects the shape and compactness of the adhering aggregates, the efficiency of shear forces upon cleaning and finally, the adherence of soiling particles. The presence of proteins (either native or denatured) at the interface improved strongly the cleanability of more hydrophilic substrate (glass, StSteel-UVO). This is attributed to the lower surface tension. The dependence of cleanability on capillary forces, and in particular on the liquid surface tension, is predominant as compared with its dependence on the size and shape of the soiling aggregates, which influence the efficiency of shear forces exerted by the flowing water upon cleaning. The cleanability of less hydrophilic substrate (stainless steel only pre-cleaned with ethanol) did not change markedly in the presence of proteins; this may be due to a more complex interaction between surface tension and contact angle, on one hand, and a more complex interaction between proteins and contaminants, on the other hand. The presence of dextran did not affect the cleanability, as neither the liquid surface tension nor the contact angle was appreciably affected. [less ▲]

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See detailImpact of goldfish on terrestrial and aquatic microhabitat use in the palmate newt
Darnet, Elodie; Winandy, Laurane ULiege; Denoël, Mathieu ULiege

Poster (2014, December 12)

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See detailImpact of triclosan on behaviour and neural development of Cyprinodon variegatus
Benichou, Farida; Rahmouni, Chahrazed; Denoël, Mathieu ULiege et al

Poster (2014, December 12)

The study focussed on the effects of triclosan (TCS) exposure on mobility and hearing capacities of Cyprinodon variegatus larvae. TCS is an omnipresent antimicrobial and contaminant of aquatic ecosystems ... [more ▼]

The study focussed on the effects of triclosan (TCS) exposure on mobility and hearing capacities of Cyprinodon variegatus larvae. TCS is an omnipresent antimicrobial and contaminant of aquatic ecosystems, which can act as endocrine disruptor, mainly by modifying thyroid functioning. Larval stages are particularly vulnerable to deleterious effects of endocrine disrupters because of potential impairment of fish development and behaviour. Exposure to TCS was conducted at fertilization of eggs at concentrations likely to be found in the environment: 20, 50 and 100 μg.l-1. The analysis of growth parameters of C. variegatus showed no effect of TCS on the fertility of eggs, survival and larval weight. Subsequently, THs concentrations were measured on 15 days post hatching larvae. THs are initially produced as T4 (thyroxine) cells and then converted in the bioactive form of T3 (triiodothyronine) cells. The observed increase of T4 and T3 cells in larvae exposed to 50 and 100 μg.l-1 suggests an increase in THs synthesis as a consequence of TCS exposure. Auditory thresholds of larvae were determined using ABR (Auditory Brainstem Response) technique, and finally larval mobility was measured. For both parameters no significant differences were observed among the three different treatments. Audiograms showed that the auditory system is not yet completely established at 30 days post hatching. However, these results allowed us to consider C. variegatus as an “hearing generalist” because this species have a hearing sensitivity lower than 2000 Hz. Regarding locomotion, our result summarized short time experiences targeting only swimming speed, distance and degree of mobility. It would be interesting to expand the behavioural aspects on other parameters of locomotion and integrate Cyprinodon reaction to different stress (light or touch). In conclusion, our results require an extensive long-term study on the full life cycle of C. variegatus, in order to evaluate the impact of triclosan on neural function and behaviour through several generations. [less ▲]

Detailed reference viewed: 57 (5 ULiège)