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See detailAnaerobic methane oxidation and aerobic methane production in an east African great lake (Lake Kivu)
Roland, Fleur ULiege; Morana, Cédric; Darchambeau, François et al

in Journal of Great Lakes Research (2018)

We investigated CH4 oxidation in the water column of Lake Kivu, a deep meromictic tropical lake with CH4-rich anoxic deep waters. Depth profiles of dissolved gases (CH4 and N2O) and a diversity of ... [more ▼]

We investigated CH4 oxidation in the water column of Lake Kivu, a deep meromictic tropical lake with CH4-rich anoxic deep waters. Depth profiles of dissolved gases (CH4 and N2O) and a diversity of potential electron acceptors for anaerobic CH4 oxidation (NO3−, SO4 2−, Fe and Mn oxides) were determined during six field campaigns between June 2011 and August 2014. Denitrification measurements based on stable isotope labelling experiments were performed twice. In addition, we quantified aerobic and anaerobic CH4 oxidation, NO3− and SO4 2− consumption rates,with and without the presence of an inhibitor of SO4 2−-reducing bacteria activity. Aerobic CH4 production was also measured in parallel incubations with the addition of an inhibitor of aerobic CH4 oxidation. Themaximu m aerobic and anaerobic CH4 oxidation rates were estimated to be 27±2 and 16±8 μmol/L/d, respectively. We observed a difference in the relative importance of aerobic and anaerobic CH4 oxidation during the rainy and the dry season, with a greater role for aerobic oxidation during the dry season. Lower anaerobic CH4 oxidation rates were measured in presence of molybdate in half of the measurements, suggesting the occurrence of linkage between SO4 2− reduction and anaerobic CH4 oxidation. NO3− consumption and dissolved Mn production rates were never high enough to sustain themeasured anaerobic CH4 oxidation, reinforcing the idea of a coupling between SO4 2− reduction and CH4 oxidation in the anoxic waters of Lake Kivu. Finally, significant rates (up to 0.37 μmol/L/d) of pelagic CH4 production were also measured in oxygenated waters. [less ▲]

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See detailL'image exposée. La représentation des photographies de l'Agence France-Presse sur les réseaux sociaux numériques
Fabre, Maxime ULiege

Doctoral thesis (2018)

Le cliché d’une « barquette de frites nappées de chocolat », un « coucher de soleil » aux couleurs saturées près de Puerto Natales au Chili… Cette thèse a pour objectif d’étudier la représentation des ... [more ▼]

Le cliché d’une « barquette de frites nappées de chocolat », un « coucher de soleil » aux couleurs saturées près de Puerto Natales au Chili… Cette thèse a pour objectif d’étudier la représentation des photographies exposées par l’Agence France-Presse sur les « réseaux sociaux » – Instagram, Facebook, Twitter et Tumblr en têtes –, à l’aune d’une réflexion sur les transformations et les mutations des pratiques photojournalistiques en régime « numérique ». L’étude se divise en deux axes : une approche sémiotique d’abord, qui permet de comprendre de quelle manière le discours photojournalistique déploie les conditions formelles et médiatiques qui président à la fixation de la croyance, et articulent des normes et des attentes sur le statut de vérité du monde présenté par ces images ; une approche généalogique ensuite, qui étudie les conditions selon lesquelles les photographies de presse sont exposées. En réinterrogeant le concept de banalité, en lui redonnant son sens premier, ce deuxième axe développe une réflexion sur la complexité de la médiation « numérique », entre le pouvoir de réquisition des « industries du passage » d’un côté, et l’adaptation du discours informationnel de l’AFP de l’autre. À partir d’un point de vue nettement interdisciplinaire, la thèse prouve ici qu’il est possible de comprendre la médiation « numérique » comme la corrélation expressive entre l’économie d’un dispositif et le développement de formes de vies iconiques singulières. [less ▲]

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See detailOf Buying Many Books There is No End: Paratextual Marks as a Source for the History of the Book in Islam
Bauden, Frédéric ULiege

Scientific conference (2018, December 17)

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See detailMolecular breeding and gene function verifying for cucumber fruit traits
Hu, Bowen ULiege

Doctoral thesis (2018)

Cucumber is a widely cultivated vegetable with high economic benefit. The assembled draft genome sequence and variation map generated by deep resequencing provided big data to advantage cucumber serving ... [more ▼]

Cucumber is a widely cultivated vegetable with high economic benefit. The assembled draft genome sequence and variation map generated by deep resequencing provided big data to advantage cucumber serving as the model plant for sex determination, long-distance trafficking and plant viruses disease research. However, the underlying molecular basis of some important horticultural traits, such as peel color, is still unknown. Besides, Cucurbitaceous species are well known to be one of the most refractory plants for transformation, which makes many meaningful traits hardly to be verified reliably in cucumber by reverse genetics, or makes identified gene cannot be applied well in molecular breeding. In this dissertation, the classical research methods of forward genetics and reward genetics was adopted. Initially, we discovered a cucumber mutant showing distinct light green exocarp of immature fruit from our EMS mutant library. By combining bulked-segregant analysis (BSA) and transient RNA interference, we first identified Csa7G051430 responsible for light green skin. Additionally, we successfully obtained transgenic cucumber using an inbred line rather than an F1 hybrid as previously used. We further optimized the CRISPR/Cas9 system by using stronger CsU6 promoter and a GFP tag to facilitate selection both the transformants and transgene-free mutants among the progeny. Thirdly, with these optimized procedures, we first verified the gene function of CsWIP1 which plays an important role in sex determination in cucumber and generated transgene-free gynoecious cucumber plants from a commercially valuable inbred line. The main contents and results are as follows: (1) An accumulation and replication of chloroplasts 5 gene mutation A mutant showing light green exocarp was discovered from ethylmethane sulfonate (EMS) mutagenized cucumber line 406 with dark green exocarp. Genetic analysis showed the mutant phenotype is conferred by a single recessive gene, here designated as lgp (light green peel). By re-sequencing of bulked segregants, we identified the candidate gene Csa7G051430 encoding ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 (ARC5) that plays a vital role in chloroplast division in Arabidopsis. A single nucleotide polymorphism (SNP) causing amino acid alteration in the conserved GTPase domain of Csa7G051430 showed co-segregation with the altered phenotype. Furthermore, a transient RNA interference of this gene resulted in reduced number and enlarged size of chloroplasts, which were also observed in the lgp mutant. These evidences support that the non-synonymous SNP in Csa7G051430 is the causative mutation for the light green peel. This study provides a new allele for cucumber breeding for light green fruits and additional resource for the study of chloroplast development. (2) Improving transformation protocol and optimizing CRISPR/Cas9 system in cucumber To find a way to improve the genetic transformation efficiency, GFP (green fluorescent protein) was used as a reporter during Agrobacterium infection and in situ hybridization of CsSTM was carried out. The result suggested the regenerated adventitious shoot was originate from cells in deeper layers where Agrobacterium can barely arrived by immerse infection. To enhance infection, a simple syringe was used for vacuum infiltration. Additionally, hemin was used to promote the rooting of transgenic shoots, which solved the problem that addition of auxin promoted chlorosis of the transgenic shoots. The transformation protocol established in this study was used to perform CRISPR/Cas9-mediated knocking out of three genes and the transformation efficiency approached 1.00‰. Aside from cucumber, the transgenic and genome-editing approach has been validated in melon, so it would be possible that this approach can be widely used in Cucurbitaceae species. (3) Engineering non-transgenic gynoecious cucumber using CRISPR/Cas9 system We verified the function of an important sex determination gene CsWIP1 and obtained transgene-free gynoecious cucumber by utilizing the improved efficient transformation protocol and optimized CRISPR/Cas9 system. Cswip1 T0 mutants displayed gynoecious phenotype, with the upper nodes bearing only female flowers, which indicated CsWIP1 acts as an inhibitor of carpel development in cucumber. Three types of deletions were found in T0 plant line 4 which displayed a high mutation rate 64.3% without off target. Homozygous and transgene-free Cswip1 T2 mutants were obtained from self-pollinating of heterozygous T1 mutants generated by crossing-pollinating between T0 and wild-type. Compared with monoecious wild-type plants, the Cswip1 mutants had seven times female flowers more than wild-type. Development of gynoecious cucumber by transgenic approach provide a stable and fast strategy to convert monoecious lines to gynoecious lines. [less ▲]

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See detailWhat do the Brazilian piranhas have to tell us?
Raick, Xavier ULiege; Huby, Alessia ULiege; Kurchevski, Gregório et al

Conference (2018, December 15)

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See detailLe Temple et le cantique de Syméon en Luc 2, 29-32. Analyse d’une métaphore poétique opérée par l’auteur du 3e Évangile
Bassilua Nzuzi, Anicet ULiege

Conference (2018, December 15)

The Bible contains a number of religious songs designated by Hebrew terms, then Greek (hymnos, ôdè, psalmos), often of similar meaning. The Old Testament provides many examples through the Psalter. In the ... [more ▼]

The Bible contains a number of religious songs designated by Hebrew terms, then Greek (hymnos, ôdè, psalmos), often of similar meaning. The Old Testament provides many examples through the Psalter. In the New Testament, it is attested (Lehmann, 2009) that Jesus and his disciples had an intimate knowledge of the Psalms. These songs of the Old Testament were particularly used in the liturgy of the Temple of Jerusalem, especially for sacrifices, feasts, processions. Their use has even been extended to the field of private religion (Ibid.). It turns out that these texts inspire some others in the New Testament, like the four hymns that appear at the beginning of Luke's gospel (1,46-55: Magnificat; 1,68-79: Benedictus 2, 14: Gloria: and 2, 29-32: Nunc Dimittis). Of these four hymns, that of Simeon (the Nunc Dimittis) is pronounced in the Temple thus opening a track that links the capture of its contours in consideration of the context of its execution, the sanctuary. We propose to analyze this link. Indeed, we know that the first two chapters of the Gospel of Luke function as the « narrative beginning » of the entire « work to Theophilus » - Luke-Acts - (Gerber, 2009) and that in these two chapters the information provided on the birth of Jesus was carefully chosen and placed as the foundation of God's soteriological project presented in this book. As a result, the four songs contained in this sequence are part of this literary strategy of the author of Luke (Dillon, 2006) who, unlike Paul or Marc who present the work of salvation through the theology of the cross (ie the death of Jesus), places the moment of salvation in his birth. This birth is thus declined in words, miracles and acts of compassion (Marguerat, 2008) who call, for the characters chosen by Luke, the praise. This is how these words are placed especially in the mouth of Simeon. And their significance deserves a stop for a moment. The formula εὐλόγησεν τὸν θεὸν (v. 28) placed at the beginning of the hymn seems to indicate that the following point of view is that of the author (Lohfink, 1994); the verb ὁράω (see) which qualifies the wonder realized by God vis-à-vis Simeon does not appear as a fruit of chance, etc. As it can be seen, the combination of spoken words with the character who pronounces it (Simeon, pious man) - obviously the song of Simeon has a parallel in the book of Jubilee (Jub 22: 7-9) and in Cicero ( The Republic, VI, 9) - and the place where they are pronounced (the Temple) tends to suggest an additional layer - if not the main one - of the meaning that Luke proposes to Nunc Dimittis at least in the understanding that he wants to give to this event occurred at the Temple (the meeting between Jesus and Simeon). By putting this text in parallel with others of the Old Testament - the New Testament provides many cases of proofreading of the ancient texts operated by the first Christians (1st - 2nd Century) - as the one who reports the vision (see) of Ezekiel of the departure of the glory of God from the Temple (Ez, 10), we find an illumination of this soteriological dimension that Luke proposes in his work. Jesus, qualified of your salvation (τὸ σωτήριόν σου) in the mouth of Simeon, is seen as the return of the glory of God in the same Temple. So, we can clearly see that, starting from these elements, the topological anchoring of the performance of Simeon's song, the Temple, makes the latter (the Temple) a metaphor of the hymn itself. [less ▲]

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See detailModeling non-stationary operational risk: A smooth-transition distributional regression approach
Hambuckers, Julien ULiege; Kneib, Thomas

Conference (2018, December 15)

The distribution of operational losses is particularly challenging to model, due to the high probability of extremes and the existence of time-varying structural dependencies. In particular, operational ... [more ▼]

The distribution of operational losses is particularly challenging to model, due to the high probability of extremes and the existence of time-varying structural dependencies. In particular, operational loss severity distribution is often concerned with changes in regulations, business cycles or financial crises that affect the dependence structure with potential predictors. To help accounting for this empirical feature, we introduce smooth-transition (ST) Generalized Pareto (GP). In this time-varying regression model, the parameters of the GP distribution are related to explanatory variables through a regression function, which depends itself on a time-varying predictor of structural changes. First, we discuss the computational challenges associated to this class of models. Then, we propose several estimation strategies and investigate their finite sample properties in a simulation study. Eventually, we use our findings to study the time-varying dependence structure of monthly operational risks with market volatility and past extreme events. [less ▲]

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See detailLes dangers de l'hyper-spécialisation précoce
Jidovtseff, Boris ULiege

Scientific conference (2018, December 15)

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See detailMagnetocapillary Self-Assemblies: Interfacial Locomotion at Low Reynolds Number
Grosjean, Galien ULiege

Doctoral thesis (2018)

Les auto-assemblages magnétocapillaires se forment lorsque des particules ferromagnétiques douces sont déposées sur la surface d'un liquide et soumises à un champ d'induction. Ils émergent de la ... [more ▼]

Les auto-assemblages magnétocapillaires se forment lorsque des particules ferromagnétiques douces sont déposées sur la surface d'un liquide et soumises à un champ d'induction. Ils émergent de la compétition entre une interaction dipolaire magnétique et l'effet Cheerios, une force attractive due à la déformation du ménisque. Sous des champs variables, ces structures auto-assemblées nagent le long de l'interface. Grâce à leur simplicité conceptuelle, ils permettent de vérifier et d'étendre notre compréhension générale des micronageurs. Différents arrangements de particules peuvent être réalisés dans le but d'accomplir divers objectifs : du plus simple nageur unidimensionel à la plus complexe structure inspirée du vivant ; d'un nageur rapide et contrôlable à un transporteur de cargaison ou mélangeur de fluides. [less ▲]

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See detailSynthesis and AFM-based Single-Molecule Force Spectroscopy of Helical Aromatic Oligoamide foldamers
Devaux, Floriane ULiege

Doctoral thesis (2018)

In this work, we describe the synthesis of aromatic oligoamide foldamers designed for AFM-based single-molecule force spectroscopy (SMFS) and the detailed investigation of their mechanical properties by ... [more ▼]

In this work, we describe the synthesis of aromatic oligoamide foldamers designed for AFM-based single-molecule force spectroscopy (SMFS) and the detailed investigation of their mechanical properties by this technique. The aim of the thesis was to gain insights into the forces governing the folding and the mechanochemical properties of synthetic folded molecules. We designed and studied three different kinds of helical foldamers to investigate the influence of the size of the foldamer and of the chemical architecture on the mechanical properties at the level of the single molecule in organic solvents. Pulling experiments on quinoline-based foldamers of four different sizes revealed a characteristic unfolding pattern, consistent with the sequential opening of the same groups of interactions involved in the helical conformation. The length of the unfolding patterns is proportional to the foldamer size, while the average unfolding forces show non-linear force dependence. This result highlighted the cooperativity of the interactions originating from the chemical sequence in the folding. From pulling-relaxing SMFS experiments, we evaluated the unfolding free energies and evidenced the capacity of the unfolded molecules to rapidly refold under the mechanical load. Together with experiments in different solvents, these results demonstrate the high robustness of this class of foldamers. SMFS experiments on the naphthyridine-constituted foldamer confirmed its helical structure and suggested that the strength of the interactions along the foldamer are weaker for a wider helix. The insertion of a methylene bridge in the aromatic oligoamide sequence showed a substantial effect on the mechanical properties. The aliphatic junction indeed disrupts the interaction continuum in the helical conformation under load. Providing detailed characterizations down to the single-molecule level, which are complementary to the traditional ensemble experiments, we also showed that helically folded aromatic oligoamides are a genuine class of foldamers with very promising properties as building blocks for mechanized nanomachines. [less ▲]

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See detailInterpretatie van het EVRM: 'nationale veiligheid'
Notermans, Pierre ULiege

Poster (2018, December 14)

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See detailHow much do we rely on additivity and linearity of aerodynamic and aeroelastic loading models ?
Denoël, Vincent ULiege

Scientific conference (2018, December 14)

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See detailPREGNANCY AND ENDOCRINE DISORDERS
VALDES SOCIN, Hernan Gonzalo ULiege

Learning material (2018)

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See detailHow to Support Environmental Protection Policy in Agriculture: A Case Study in Henan and Hebei Provinces, China
Yang, Xiaomei ULiege

Doctoral thesis (2018)

Crop straw (agricultural residue) is one of the most important biomass resources in China. Crop straw is either burned in the field or collected for recycling. Open burning of crop straw releases ... [more ▼]

Crop straw (agricultural residue) is one of the most important biomass resources in China. Crop straw is either burned in the field or collected for recycling. Open burning of crop straw releases particulate matter and gaseous pollutants, which play a key role in poor air quality, prompting heavy haze episodes during the harvest season. Such episodes threaten human health and interfere with social and economic activities. In contrast, recycling of crop straw reduces open burning and avoids its negative environmental impacts. In fact, improving the efficiency of straw use contributes to a circular economy, dedicated to reducing waste, while also making the best use of any ‘waste’ in economically viable processes that increase its value. Returning straw to agricultural fields in China is the easiest solution and the most important measure promoted by governments promising clean technologies to replace open burning. Recently, China’s municipalities have issued regulations forbidding outdoor burning of straw to reduce air pollution and have passed regulations to encourage farmers to use straw shredders during harvesting, and return crop straw as a bio-fertilizer. However, these regulations have not achieved the desired results, with ongoing open burning and reluctant use of straw on fields. In the first part of this research, urban residents’ willingness of to pay (WTP) for a corn straw ban in Henan (China) was assessed using contingent valuation in a face-to-face survey. Such assessments are important for policy makers to determine the investment and policy instruments for regulating the environmental impacts of straw open burning. The expected WTP analyzed using the Tobit model was about 77 RMB per person per year for the total respondents and 143 RMB per person per year for respondents with positive WTP bids. Aggregate values were between 3.4 and 3.9 billion RMB, suggesting that the corn straw burning ban is of considerable economic value in Henan. In the second part of this research, the factors affecting farmers’ willingness to participate in corn straw return and their willingness to accept compensation (WTA) were explored using a questionnaire survey and face-to-face interviews. A logistic regression model was used to assess adoption success, and the Tobit model was used for WTA analysis. High machinery costs, amount of straw returned, and slow decomposition rates of straw were the most significant factors negatively influencing adoption of this practice. They had a positive influence on the WTA. Poor quality of the straw was another significant factor reducing the probability of using straw return technology. Sown areas and soil improvements associated with adding straw wereoth positive factors determining adoption of the practice and negative determinants affecting WTA compensation. The mean WTA for the total respondent sample was 47 RMB per mu. In the third part of this research, a field experiment was carried out to compare the effects of tillage (minimum/full tillage) combined with corn straw return (mulching, incorporation, and removal) and irrigation (reduced/normal irrigation) methods on wheat productivity and water conservation. In 2013-2014, the yield for minimum tillage with residue mulch (MTm) was slightly but not significantly higher than the yield under full tillage with residue incorporation (FTi). Yields for MTm with reduced irrigation were 10.2% higher than FTi and reduced irrigation. The positive crop response to MTm may reflect higher topsoil moisture and soil temperature under MTm compared with FTi during winter. In conclusion, this study showed there is huge value to prohibiting open burning of corn straw to improve air quality. Despite machinery and operational problems that negatively influence farmers’ enthusiasm for straw return, minimum tillage coupled with corn straw return does benefit subsequent wheat yields. [less ▲]

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See detailThe Admissibility of Multiple Human Rights Complaints: Strasbourg and Geneva Compared
Deprez, Christophe ULiege

Conference (2018, December 14)

Forum duplication in international human rights litigation is closely connected to the general debate on the proliferation and (in)efficiency of the global system of human rights protection. This ... [more ▼]

Forum duplication in international human rights litigation is closely connected to the general debate on the proliferation and (in)efficiency of the global system of human rights protection. This presentation sought to provide a practical, comparative overview of the applicable rules and relevant case law of the European Court of Human Rights ('ECtHR') and of the UN Human Rights Committee ('HRCee') on this issue. While specific inadmissibility clauses have been drafted within these two regimes with a view to preventing multiple human rights petitions in relation to the same matter, their respective scopes differ. Moreover, both in Strasbourg and Geneva, the applicable normative framework has led to important judicial developments which deserve to be thoroughly addressed. [less ▲]

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See detailRelire la charte de Venise à travers les archives de R.M. Lemaire: genèse, révisions, questionnements
Houbart, Claudine ULiege

Scientific conference (2018, December 13)

Déposées en 1992 aux archives universitaires de la KULeuven, les archives de Raymond M. Lemaire (1921-1997) constituent une ressource exceptionnelle pour l’histoire de la conservation-restauration dans la ... [more ▼]

Déposées en 1992 aux archives universitaires de la KULeuven, les archives de Raymond M. Lemaire (1921-1997) constituent une ressource exceptionnelle pour l’histoire de la conservation-restauration dans la seconde moitié du XXe siècle. Outre ses projets personnels et son implication au sein d’organismes internationaux tels que l’ICOMOS, le Conseil de l’Europe et l’Unesco, la « fabrique » de plusieurs documents internationaux y est documentée. Parmi ceux-ci, la charte de Venise, dont Lemaire fut l’un des rédacteurs avant de tenter à plusieurs reprises, et en vain, d’en obtenir une révision. Cet exposé abordera tant la genèse que les tentatives de révision de la charte, en parallèle avec les projets contemporains de R.M. Lemaire et l’émergence de nouveaux concepts au cours des années 1970, telle la conservation intégrée. [less ▲]

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See detailObservations of satellite footprints in Jupiter's Aurorae
Mura, A.; Adriani, A.; Altieri, F. et al

Conference (2018, December 12)

JIRAM (Jovian Infrared Auroral Mapper) on board Juno is an imager/spectrometer in the 2-5 um range. One imaging channel is designed to study the Jovian H3+ auroral emissions. Its high angular resolution ... [more ▼]

JIRAM (Jovian Infrared Auroral Mapper) on board Juno is an imager/spectrometer in the 2-5 um range. One imaging channel is designed to study the Jovian H3+ auroral emissions. Its high angular resolution, combined with the unique vantage point provided by Juno, allows JIRAM to observe the aurorae in unprecedented detail. Here we present the results from ~2 years of observations of the auroral footprints of the Galilean moons. These are bright spots and associated tail that appear in Jupiter’s ionosphere at the base of the magnetic field lines which sweep past Io, Europa, and Ganymede. The moons are obstacles in the path of Jupiter’s rapidly rotating magnetospheric plasma, and the resulting electromagnetic interaction launches Alfvén waves along the magnetic field towards Jupiter, where intense electron bombardment of the hydrogen atmosphere causes it to glow. Recent observations reveal for the first time that the footprint of Io is comprised of a regularly spaced array of emission features, extending downstream of the leading footprint. Contrary to the larger spots seen in lower resolution images, the small scale of these multiple features (~100 km) is incompatible with the simple paradigm of multiple Alfvén wave reflections. Additionally, observations of Io’s trailing tail well downstream of the main footprint reveal a pair of closely spaced parallel arcs, previously unresolved. The temperatures of the main spot and tail, retrieved with the JIRAM spectrometer, are lower than the main auroral oval. This could indicate that the emission is located at a deeper level, possibly caused by higher energy electrons. Ganymede’s footprint spots (main and secondary) appear as a pair of emission features that provide a remote measure of the size Ganymede’s magnetosphere, mapped from its distant orbit onto Jupiter’s magnetosphere. [less ▲]

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See detailTraitements pharmacologiques après le coma
Sanz, Leandro ULiege

Conference given outside the academic context (2018)

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