References of "Pastoret, Paul-Pierre"
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See detailVirus neutralising antibodies against 22 bovine viral diarrhoea virus isolates in vaccinated calves
Hamers, Claude ULiege; Di Valentin, Emmanuel ULiege; Dumont, Chloé ULiege et al

in Veterinary Journal (2002), 163(1), 61-67

Seven of nine colostrum deprived calves, free from bovine viral diarrhoea virus (BVDV), were vaccinated with a commercially available vaccine containing two inactivated strains of BVDV, an inactivated ... [more ▼]

Seven of nine colostrum deprived calves, free from bovine viral diarrhoea virus (BVDV), were vaccinated with a commercially available vaccine containing two inactivated strains of BVDV, an inactivated strain of bovine herpesvirus-1 and modified-live strains of bovine respiratory syncytial virus and para-influenza-3 virus. The two other calves were kept as controls. The virus neutralising (VN) antibodies induced by vaccination were tested against 22 antigenically diverse BVDV isolates, including reference strains and field isolates, both cytopathic and non-cytopathic, as well as genotypes I and II. The strains were isolated in Belgium, France, Germany, the United Kingdom and the USA. While there were variations in the VN titres of the individual calves against all the strains, serum from the seven animals neutralised 20 or more of the strains tested. From the results, it can be concluded that the vaccine can stimulate the production of VN antibodies capable of neutralising a wide range of European and American isolates of BVDV, including genotypes I and II. [less ▲]

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See detailNew vaccine production technologies and their impact on the use of animals
Pastoret, Paul-Pierre ULiege; Sitrin, R.; Merten, O. W. et al

in Developments in Biologicals (2002), 111

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See detailCostimulatory molecule requirement for bovine WC1+ γδ T cells' proliferative response to bacterial superantigens
Fikri, Youssef; Pastoret, Paul-Pierre ULiege; Nyabenda, Jean

in Scandinavian Journal of Immunology (2002), 55(4), 373-381

We have previously shown that the proliferation of freshly isolated bovine WC1+ γδ T cells to superantigens (SAgs) including staphylococcal enterotoxin A (SEA), and staphylococcal enterotoxin B (SEB) or ... [more ▼]

We have previously shown that the proliferation of freshly isolated bovine WC1+ γδ T cells to superantigens (SAgs) including staphylococcal enterotoxin A (SEA), and staphylococcal enterotoxin B (SEB) or toxic shock syndrome type-1 (TSST-1) required the presence of antigen-presenting cells (APC) and the addition of exogenous interleukin (IL)-2. The costimulatory activity provided by molecules expressed on professional APC for the proliferation of γδ T cells has not been addressed hitherto. In the present study, we investigated the ability of two selected APC populations, the dendritic cells (DCs) highly expressing CD80 and CD86 molecules (CD80highCD86high) and the monocytes expressing the same molecules at a rather low level (CD80lowCD86low), to stimulate the proliferation of purified bovine WC1+ γδ T cells to SAgs. DCs were more efficient than monocytes in inducing γδ T-cell proliferation, and this response was dependent on exogenous IL-2 in both presentation modes. Stimulating γδ T cells with gradual doses of SAgs or concanavalin A (ConA) resulted in similar dose-dependent reaction profiles suggesting a minimal role of the major histocompatibility complex (MHC). However, significant proliferation was already obtained with the starting doses in the presence of DC compared with monocytes, and higher proliferation was reached with DC at optimal doses. Finally, the addition of monoclonal antibody (MoAb) anti-CD86 markedly inhibited SAgs- and ConA-mediated proliferation, whereas MoAb anti-CD80 had no effect. The combination of both anti-CD80 and anti-CD86, however, suppressed this response. These results suggest that bovine γδ T-cell proliferation response requires indubitably CD86 costimulation. The role of CD80 molecule seems less clear. [less ▲]

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See detailPrésentation de Monsieur Alain Vanderplasschen : Lauréat du prix Édouard Van Beneden
Pastoret, Paul-Pierre ULiege

in Bulletin de la Société Royale des Sciences de Liège (2002), 71(2), 82-83

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See detailLa traçabilité des maladies infectieuses animales
Pastoret, Paul-Pierre ULiege

in Bulletin de la Société Royale des Sciences de Liège (2002), 71(1), 47-63

Traceability of animal infectious diseases is one of the oldest problems taken into account by veterinary officials. Regulations are put in place by the Office international des Epizooties based in Paris ... [more ▼]

Traceability of animal infectious diseases is one of the oldest problems taken into account by veterinary officials. Regulations are put in place by the Office international des Epizooties based in Paris. It was previously often impossible to distinguish between an animal infected with a wild strain of infectious agent or simply vaccinated using the available diagnostic tools. Nowadays it is sometimes possible to do it using marker vaccines associated with a companion diagnostic test. Examples can be found with classical swine fever and pseudorabies in pigs, infectious bovine rhinotracheitis, foot and mouth disease, equine influenza and, to a lesser extent, rabies and feline leukaemia. Traceability of spongiform encephalopathies will also be discussed. [less ▲]

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See detailCD40 engagement enhances eosinophil survival through induction of cellular inhibitor of apoptosis protein 2 expression: possible involvement in allergic inflammation
Bureau, Fabrice ULiege; Seumois, G.; Jaspar, F. et al

in Pflügers Archiv: European Journal of Physiology (2002), 443

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See detailCyclopentenone prostaglandins at low concetrations exert pro-inflammatory effects through oxidative stress-induced ERK1/2 activation
Bureau, Fabrice ULiege; Desmet, Christophe ULiege; Mélotte, C. et al

in Proceedings: Spring Meeting of the Belgian Society of Physiology and Pharmacology (2002)

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See detailCD40 engagement enhances eosinophil survival through induction of cellular inhibitor of apoptosis protein 2 expression: Possible involvement in allergic inflammation.
Bureau, Fabrice ULiege; Seumois, Gregory; Jaspar, Fabrice et al

in Journal of Allergy and Clinical Immunology (2002), 110(3), 443-9

BACKGROUND: CD40 engagement enhances eosinophil survival, suggesting a role for this receptor in the development of eosinophilia. OBJECTIVE: We examined whether CD40 enhances eosinophil survival by ... [more ▼]

BACKGROUND: CD40 engagement enhances eosinophil survival, suggesting a role for this receptor in the development of eosinophilia. OBJECTIVE: We examined whether CD40 enhances eosinophil survival by inducing the expression of antiapoptotic proteins. Three members of the inhibitor of apoptosis protein (IAP) family, namely cellular (c)-IAP1, c-IAP2, and XIAP, and 2 antiapoptotic proteins of the Bcl-2 family, namely Bcl-x(L) and Bfl-1/A1, were investigated. METHODS: Blood and sputum were obtained from healthy subjects and atopic asthmatic patients. Blood eosinophils were isolated by means of magnetic selection. Expression of CD40, IAPs, and Bcl-2 proteins was investigated by using flow cytometry, immunoblotting, or both. CD40 stimulation was achieved with agonistic antibodies or soluble ligands. Apoptosis was assessed by staining with propidium iodide and FITC-conjugated annexin-V. c-IAP2 expression was inhibited with antisense oligonucleotides. RESULTS: Freshly isolated eosinophils from healthy and asthmatic patients did not express CD40. Conversely, eosinophils expressed CD40 spontaneously when cultured for 48 hours. At this time point, CD40 stimulation significantly delayed eosinophil apoptosis. Inhibition of eosinophil apoptosis was accompanied by induction of c-IAP2 but not c-IAP1, XIAP, Bcl-x(L), or Bfl-1/A1 expression. Antisense knockdown of c-iap2 abolished CD40-induced enhancement of eosinophil survival. Sputum cells from asthmatic patients, unlike those from healthy subjects, substantially expressed CD40 and c-IAP2. Moreover, a strong correlation was found between the percentage of eosinophils in the sputum from asthmatic patients and the sputum level of CD40 and c-IAP2 expression. CONCLUSION: The results demonstrate that CD40 engagement enhances eosinophil survival through induction of c-IAP2 expression and suggest a role for this mechanism in allergic inflammation. [less ▲]

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See detailGenetic Characterization of Puumala Hantavirus Strains from Belgium : evidence for a Distinct Phylogenetic Lineage
Escutenaire, Sophie; Chalon, Patrice ULiege; Heyman, Paul et al

in Virus Research (2001), 74(1-2), 1-15

Puumala hantavirus (PUUV) sequences were recovered from red bank voles (Clethrionomys glareolus) trapped between 1996 and 1998 in four localities of southern Belgium: Thuin, Montbliart, Momignies and ... [more ▼]

Puumala hantavirus (PUUV) sequences were recovered from red bank voles (Clethrionomys glareolus) trapped between 1996 and 1998 in four localities of southern Belgium: Thuin, Montbliart, Momignies and Couvin. In addition, three PUUV isolates originating from bank voles trapped in the 1980s in southern (Montbliart) and northern (Turnhout) Belgium were genetically characterized. Analysis of the complete S and partial M segment sequences showed that the Belgian PUUV strains constitute a genetic lineage, distinct from other known PUUV lineages from Europe and Japan. This lineage also includes a wild strain (Cg-Erft) originating from a neighbouring area of Germany. Within the Belgian lineage, geographical clustering of genetic variants was observed. In the Montbliart site, the range of diversity between the most temporally distant strains (from 1986 and 1996-1998) was higher than between those from 1996 and 1998, suggesting slight genetic drift via accumulation of neutral or quasi-neutral substitutions with time. [less ▲]

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See detailDiversity among Bovine Pestiviruses
Hamers, Claude ULiege; Dehan, Pierre ULiege; Couvreur, B. et al

in Veterinary journal (2001), 161(2), 112-22

Bovine viral diarrhoea virus (BVDV) isolates are characterized by an important genetic, antigenic and pathogenic diversity. The emergence of new hypervirulent BVDV strains in North America has provided ... [more ▼]

Bovine viral diarrhoea virus (BVDV) isolates are characterized by an important genetic, antigenic and pathogenic diversity. The emergence of new hypervirulent BVDV strains in North America has provided clear evidence of pathogenic differences between BVDV strains. The origin of BVDV diversity is related to high mutation rate occurring in RNA viruses but the consequences of mutations obviously depend on the genes which are involved. Mutations in genes encoding for structural proteins of immunological importance may have practical implications.Knowledge of BVDV diversity is important for understanding the wide variety of pathogenesis of diseases caused by the virus, for monitoring the epidemiology of the different types and for the design of optimum laboratory tests and vaccines.This review focuses on the origin and consequences of BVDV diversity with regard to pathogenesis, biotypes, and antigenic and genetic variations. [less ▲]

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See detailHumoral and Cell-Mediated Immune Responses of Foxes (Vulpes Vulpes) after Experimental Primary and Secondary Oral Vaccination Using Sag2 and V-Rg Vaccines
Lambot, Michel ULiege; Blasco, E.; Barrat, Jacques et al

in Vaccine (2001), 19(13-14), 1827-35

Humoral and cell-mediated immune responses of 36 captive foxes to two oral vaccines against rabies currently used for foxes in Europe were studied. The Street Alabama Dufferin (SAD) mutant Gif (SAG2 ... [more ▼]

Humoral and cell-mediated immune responses of 36 captive foxes to two oral vaccines against rabies currently used for foxes in Europe were studied. The Street Alabama Dufferin (SAD) mutant Gif (SAG2) vaccine has been selected by double mutation from the SAD virus. The vaccinia recombinant virus (V-RG) expresses the rabies glycoprotein. Both vaccines induce similar humoral and cell-mediated responses after primary and secondary oral administration. We observed a typical anamnestic response, although of a limited duration, after the booster vaccination. Therefore, our results suggested that two successive oral vaccination campaigns should not significantly improve the immunisation of foxes. Lymphocyte in vitro proliferative response to the SAD antigen highlighted the presence in blood of a T-cell specific memory 6 months after vaccination. The synthesis of several vulpine cytokines was detected in peripheral blood mononuclear cells (PBMC) stimulated by SAD antigen via reverse transcription polymerase chain amplification. The data showed a concomitant expression of interleukin (IL)-4 and interferon-gamma in PBMC of vaccinated foxes. No change was detected in the level of IL-2, IL-10 and IL-12 synthesis, whereas the pro-inflammatory cytokine tumour necrosis factor-alpha seemed involved in the activation of naive T lymphocytes. [less ▲]

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See detailLes hantavirus: Évolution moléculaire et épidémiologie de l'infection
Escutenaire, Sophie; Brochier, Bernard; Pastoret, Paul-Pierre ULiege

in Virologie (2001), 5(6), 419-429

Hantaviruses belong to the Bunyaviridae family and are responsible for human diseases called haemorrhagic fever with renal syndrome (HFRS) in Asia and Europe, and hantavirus pulmonary syndrome (HPS) in ... [more ▼]

Hantaviruses belong to the Bunyaviridae family and are responsible for human diseases called haemorrhagic fever with renal syndrome (HFRS) in Asia and Europe, and hantavirus pulmonary syndrome (HPS) in Americas. Hantaviruses are carried by wild rodents and have been co-evolving with their hosts for millions of years. The genetic diversity among hantaviruses is linked to the specificity of the reservoirs and to their geographical distribution. Host rodents are chronically infected and spread the virus in saliva, urine and faeces. Humans are generally contaminated by inhalation of viral particles from infected material. In Europe, Puumala (PUU) virus is responsible for a mild form of HFRS, called nephropathia epidemica (NE), and Dobrava (DOB) virus causes more severe symptoms that may lead to the death of the patients. Until now, no vaccine against hantaviruses has been available in Europe. [less ▲]

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See detailKinetics of humoral immune response after rabies VR-G oral vaccination of captive fox cubs (Vulpes vulpes) with or without maternally derived antibodies against the vaccine.
Blasco, E.; Lambot, Michel ULiege; Barrat, Jacques et al

in Vaccine (2001), 19(32), 4805-15

In western Europe during the spring, the largest proportion of fox populations are cubs and the key to successful rabies oral vaccination campaigns is cub vaccination. In this paper we report on studies ... [more ▼]

In western Europe during the spring, the largest proportion of fox populations are cubs and the key to successful rabies oral vaccination campaigns is cub vaccination. In this paper we report on studies of the serology of 93 fox (Vulpes vulpes) cubs born to unvaccinated and orally vaccinated captive vixens, some of which were orally vaccinated at 30 or at 90 days of age with the vaccinia recombinant vaccine (VR-G) that expresses the rabies virus glycoprotein. The duration of cub passively acquired antibody, the development of immune responses to oral vaccination at either 30 or 90 days of age, possible interference between passive and active immunity to such vaccination and resistance to a potentially lethal rabies challenge dose when five months old were measured. The study showed that rabies neutralising antibody can be passed to their cubs by vixens orally vaccinated with VR-G during pregnancy. Maternally derived antibody titres in cubs declined with time and disappeared by 45-75 days after birth. Thirty days old cubs serologically responded to oral vaccination. No interference between antibody of maternal origin and active immunity conferred by VR-G oral vaccination or between antibody of maternal origin and protection was observed. Thus, very young cub immunisation against rabies with VR-G per os is possible whatever the immune status of their mothers. Provided a vaccine-bait suitable for such young cubs exists, oral vaccination at den entrances with VR-G is a feasibility. [less ▲]

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See detailAvancées récentes en biologie moléculaire du virus de la diarrhée virale bovine
Dehan, Pierre ULiege; Hamers, Claude ULiege; Couvreur, B. et al

in Annales de Médecine Vétérinaire (2001), 145(1), 39-46

This review highlights new information on molecular biology of bovine viral diarrhea virus and other pestiviruses. The intensive research on this subject has profound effects on our understanding of the ... [more ▼]

This review highlights new information on molecular biology of bovine viral diarrhea virus and other pestiviruses. The intensive research on this subject has profound effects on our understanding of the pathogenesis of the bovine viral diarrhea and the mucosal disease, leading to the development of new techniques for diagnosis and vaccination. [less ▲]

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See detailPurified bovine WC1+ γδ T lymphocytes are activated by staphylococcal enterotoxins and toxic shock syndrome toxin-1 superantigens: Proliferation response, TCR Vγ profile and cytokines expression
Fikri, Youssef; Denis, Olivier; Pastoret, Paul-Pierre ULiege et al

in Immunology Letters (2001), 77(2), 87-95

In this study, the ability of purified bovine γδ T cells in vitro to be activated by superantigens (SAg) was investigated. Freshly isolated WC1+ γδ T cells, in the presence of autologous glutaraldehyde ... [more ▼]

In this study, the ability of purified bovine γδ T cells in vitro to be activated by superantigens (SAg) was investigated. Freshly isolated WC1+ γδ T cells, in the presence of autologous glutaraldehyde-fixed or γ-irradiated antigen presenting cells (APC) and IL-2, were incubated with staphylococcal enterotoxins A and B (SEA and SEB), and toxic shock syndrome toxin-1 (TSST-1). Both a proliferative response and the expression of particular T cell receptor genes of the γ variable (TCR Vγ) repertoire family were induced. Genes encoding TCR Vγ1 and TCR Vγ2 family, but not TCR Vγ5 were detected. The cells also expressed cytokine transcripts, namely, those of IL-12, IFN-γ and TNF-α, but not IL-2, IL-4, IL-6, IL-7 and IL-10. The activation and proliferation of freshly isolated γδ T cells by non-processed antigens required two signals, one originating from the APC and a second dependent on exogenous IL-2. Our results show that purified bovine WC1+ γδ T cells could be driven to proliferate and to express a particular TCRVγ profile in response to superantigen activation. The possible implication of cytokines expressed by bovine γδ T cells in immunopathogenesis is discussed. © 2001 Elsevier Science B.V. [less ▲]

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See detailEpidemiologie de l'infection par hantavirus en Belgique
Escutenaire, S.; Pastoret, Paul-Pierre ULiege

in Bulletin et Mémoires de l'Académie Royale de Médecine de Belgique (2001), 156(1-2), 137-44144-6

In Europe, Puumala (PUU) is a hantavirus responsible for a human disease called nephropathia epidemica and its natural reservoir is the red bank vole, (Clethrionomys glareolus). Although the population ... [more ▼]

In Europe, Puumala (PUU) is a hantavirus responsible for a human disease called nephropathia epidemica and its natural reservoir is the red bank vole, (Clethrionomys glareolus). Although the population densities and the prevalence rates of infection were high in red bank voles in southern Belgium during the 1996 and 1999 epidemic years, the percentages of infected rodents were low in 1997 and 1998, when only a few positive sites were found. Antibodies against PUU virus were mainly detected in the red bank vole but also in the wood mouse (Apodemus sylvaticus) and the red fox (Vulpes vulpes). The analysis of genomic sequences has shown that the Belgian viruses and the German strain Erft constitute a genetic lineage well separated from the other European PUU strains. [less ▲]

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See detailLa maladie des vaches folles et le nouveau variant de la maladie de Creutzfeldt-Jakob.
Pastoret, Paul-Pierre ULiege

in Revue Médicale de Liège (2001), 56(8), 567-71

Bovine spongiform encephalopathy and the new variant of Creutzfeldt-Jakob disease (vCJD) belong to a family of similar diseases under the name of transmissible spongiform encephalopathies (TSE). It is ... [more ▼]

Bovine spongiform encephalopathy and the new variant of Creutzfeldt-Jakob disease (vCJD) belong to a family of similar diseases under the name of transmissible spongiform encephalopathies (TSE). It is demonstrated that the agent responsible for bovine spongiform encephalopathy (BSE) is also responsible for the new variant of Creutzfeldt-Jakob in man. This contribution describes the main characteristics of the two diseases. [less ▲]

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See detailElimination de la rage en Belgique par la vaccination du renard roux (Vulpes vulpes)
Brochier, Bernard; Dechamps, Pierre ULiege; Costy, F. et al

in Annales de Médecine Vétérinaire (2001), 145(5, NOV-DEC), 293-305

The Belgian national programme for rabies elimination by fox vaccination began in 1989. Five vaccination campaigns, covering the entire infected area (10000 km(2)), were carried out from autumn 1989 until ... [more ▼]

The Belgian national programme for rabies elimination by fox vaccination began in 1989. Five vaccination campaigns, covering the entire infected area (10000 km(2)), were carried out from autumn 1989 until autumn 1991. The two first campaigns were conducted using both an attenuated rabies virus strain (SAD B19) and a vaccinia-rabies recombinant virus (V-RG) as vaccines. Since autumn 1990, the V-RG vaccine has been used exclusively. Such campaigns induced a drastic decrease in the incidence of rabies and allowed the elimination of the disease from 80% of the initial infected area. Considering the limited distribution of rabies in Belgium and in adjacent regions of neighbouring countries, successive restricted campaigns were conducted in 1992, 1993 and 1994 along the political borders only. These campaigns resulted in a new decrease in the incidence of the disease and no more rabid foxes were detected in 1993. From 1994 to 1996, rabies spread again from a border residual focus reinfecting a 3000 km(2) previously freed area. Thanks to a modified strategy of vaccination and a good crossborder cooperation, the newly reinfected focus could be controlled. The last rabies case was a bovine recorded on the 17th Of July 1999 in the administrative entity of Bastogne, close to the Grand Duchy of Luxemburg. The last case of fox rabies was recorded on the 3(rd) of April 1998 in the same entity. According to the International Office of Epizootics guidelines (1997) and the World Health Organization recommandations (1992), Belgium, conjointly with the Grand Duchy of Luxemburg, should be officially declared rabies-free in 2001. [less ▲]

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See detailLa disponibilité du médicament vétérinaire
Pastoret, Paul-Pierre ULiege; Falize, Françoise

in Annales de Médecine Vétérinaire (2001), 145(5), 317-324

The lack of availability of veterinary medicinal products in the European Union is mainly due to the implementation of the European regulations concerning the establishment of maximal residue limits in ... [more ▼]

The lack of availability of veterinary medicinal products in the European Union is mainly due to the implementation of the European regulations concerning the establishment of maximal residue limits in products derived from food producing animals. This paper reproduces a communication from the Commission to the Council and the European Parliament dealing with the measures to be taken in order to alleviate the lack of availability of veterinary medicinal products within the European Union. [less ▲]

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