References of "Pastoret, Paul-Pierre"
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See detailEpidémiosurveillance de l'encéphalopathie spongiforme bovine en Belgique : Bilan de l'année 1999
Saegerman, Claude ULiege; Dechamps, P.; Roels, S. et al

in Annales de Médecine Vétérinaire (2001), 145(1), 47-58

[No abstract available]

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See detailLe diagnostic immunologique rapide des encéphalopathies spongiformes transmissibles
Pastoret, Paul-Pierre ULiege; Gouffaux, M.; Saegerman, Claude ULiege et al

in Annales de Médecine Vétérinaire (2001), 145

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See detailEpdémiosurveillance de l'encéphalopathie spongiforme bovine en Belgique : bilan de l'année 1999
Saegerman, Claude ULiege; Dechamps, P.; Roels, S. et al

in Annales de Médecine Vétérinaire (2001), 145

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See detailConstitutive NF-kappaB activity preserves homeostasis of quiescent mature lymphocytes and granulocytes by controlling the expression of distinct Bcl-2 family proteins
Bureau, Fabrice ULiege; Vanderplasschen, Alain ULiege; Jaspar, F. et al

in Proceedings: International Symposium: NF-kappaB: Regulation, Gene Expression & Disease (2001)

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See detailVirus Neutralizing Antibodies against a Panel of 18 Bvdv Isolates in Calves Vaccinated with Rispoval Rs-Bvd
Hamers, Claude ULiege; Di Valentin, Emmanuel ULiege; Dumont, Chloé ULiege et al

in Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health (2000), 47(10), 721-6

Seven of nine colostrum-deprived calves, free from infection with bovine virus diarrhoea virus (BVDV), were vaccinated with Rispoval RS-BVD on two occasions, 21 days apart, while the other two were kept ... [more ▼]

Seven of nine colostrum-deprived calves, free from infection with bovine virus diarrhoea virus (BVDV), were vaccinated with Rispoval RS-BVD on two occasions, 21 days apart, while the other two were kept as BVDV infection controls. The virus neutralizing (VN) serum antibodies induced by vaccination were tested for their ability to neutralize 18 European BVDV isolates, including laboratory reference strains and recent field isolates, both cytopathic and non-cytopathic biotypes as well as genotypes I and II. The strains were isolated in Belgium, France, Germany and the United Kingdom. While there were large variations in the vaccine-induced VN titres of the individual calves against all the strains, e.g. the titres against Osloss NCP, the European reference strain ranged from 1.7 to 6.7 (1:log2), serum from each animal was capable of neutralizing between nine and all 18 of the strains tested. Nevertheless, from the results of this study, it can be concluded that in colostrum-deprived BVDV seronegative calves, Rispoval RS-BVD can stimulate the production of VN antibodies capable of neutralizing a wide range of antigenically diverse European isolates of BVDV, including genotypes I and II. [less ▲]

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See detailDifferences in Experimental Virulence of Bovine Viral Diarrhoea Viral Strains Isolated from Haemorrhagic Syndromes
Hamers, Claude ULiege; Couvreur, Bernard; Dehan, Pierre ULiege et al

in Veterinary Journal (2000), 160(3), 250-258

In the late 1980s, a new hypervirulent and epidemic form of bovine viral diarrhoea virus (BVDV) infection appeared in North America. A similar but sporadic syndrome was later reported in Europe. To ... [more ▼]

In the late 1980s, a new hypervirulent and epidemic form of bovine viral diarrhoea virus (BVDV) infection appeared in North America. A similar but sporadic syndrome was later reported in Europe. To compare the pathogenic characters of the North American and European hypervirulent strains, we inoculated BVDV naive calves with BVDV strains isolated from haemorrhagic syndromes originating in Belgium, France and the USA. The experimental procedure comprised daily clinical examination and measurement of blood and virological parameters.The American BVD890/256 strain induced severe thrombocytopaenia, profuse diarrhoea and pneumonia in all calves, indicating that hypervirulent BVDV could be the primary infectious agent of pneumonia. Interestingly, a strong correlation was observed between the intense viraemia and a decreased platelet count. None of the European strains tested induced significant pathological signs, although isolated from cases presenting haemorrhagic syndrome. [less ▲]

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See detailEvidence of Puumala Hantavirus Infection in Red Foxes (Vulpes Vulpes) in Belgium
Escutenaire, Sophie; Pastoret, Paul-Pierre ULiege; Sjolander, Katarina Brus et al

in Veterinary Record: Journal of the British Veterinary Association (2000), 147(13), 365-6

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See detailA Multipotential Beta -1,6-N-Acetylglucosaminyl-Transferase Is Encoded by Bovine Herpesvirus Type 4
Vanderplasschen, Alain ULiege; Markine-Goriaynoff, Nicolas ULiege; Lomonte, P. et al

in Proceedings of the National Academy of Sciences of the United States of America (2000), 97(11), 5756-5761

The beta-1,6-N-acetylglucosaminyltransferase (beta1,6GnT) gene family encodes enzymes playing crucial roles in glycan synthesis. Important changes in beta1,6GnT expression are observed during development ... [more ▼]

The beta-1,6-N-acetylglucosaminyltransferase (beta1,6GnT) gene family encodes enzymes playing crucial roles in glycan synthesis. Important changes in beta1,6GnT expression are observed during development, oncogenesis, and immunodeficiency. The most characterized beta1,6GnTs in this gene family are the human (h) C2GnT-L and h-IGnT, which have core 2 [Galbeta1-->3(GlcNAcbeta1-->6)GalNAc] and I branching [GlcNAcbeta1-->3(GlcNAcbeta1-->6)Gal] activities, respectively. Recently, h-C2GnT-M was shown to be unique in forming core 2, core 4 [GlcNAcbeta1-->3(GlcNAcbeta1-->6)GalNAc], and I structures. To date, the beta1,6GnT gene family has been characterized only in mammals. Here, we describe that bovine herpesvirus type 4 (BHV-4) encodes a beta1,6GnT expressed during viral replication and exhibiting all of the core 2, core 4, and I branching activities. Sequencing of the BHV-4 genome revealed an ORF, hereafter called BORFF3-4, encoding a protein (pBORFF3-4) exhibiting 81.1%, 50.7%, and 36.6% amino acid identity with h-C2GnT-M, h-C2GnT-L, and h-IGnT, respectively. Reverse transcriptase-PCR analysis revealed that BORFF3-4 is expressed during BHV-4 replication. Expression of BORFF3-4 in Chinese hamster ovary cells directed the expression of core 2 branched oligosaccharides and I antigenic structures on the cell surface. Moreover, a soluble form of pBORFF3-4 had core 4 branching activity in addition to core 2 and I branching activities. Finally, infection of a C2GnT-negative cell line with BHV-4 induced expression of core 2 branched oligosaccharides. This study extends the beta1,6GnT gene family to a viral gene and provides a model to study the biological functions of a beta1,6GnT in the context of viral infection. [less ▲]

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See detailAnimals, Public Health and the Example of Cowpox
Pastoret, Paul-Pierre ULiege; Bennett, Malcolm; Brochier, Bernard et al

in Revue Scientifique et Technique. Office International des Epizooties (2000), 19(1), 23-32

Public attitudes towards animals are often contradictory. In developed countries where the problem of food security has been solved to a large degree, public concern is concentrated on food safety and ... [more ▼]

Public attitudes towards animals are often contradictory. In developed countries where the problem of food security has been solved to a large degree, public concern is concentrated on food safety and animal welfare, along with a debate on the social status of both domestic and wild animals. Zoonoses are often the focal point of these concerns. In developing countries (which often have the greatest biodiversity), wildlife constitutes an uncontrollable source of often unknown zoonoses. The authors attempt to analyse the link between animals and public health. Special attention is given to the example of cowpox, a disease which has been recognised as a zoonosis for more than two centuries but the epidemiology of which has recently been rediscovered. [less ▲]

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See detailHantavirus Infections
Escutenaire, Sophie; Pastoret, Paul-Pierre ULiege

in Revue Scientifique et Technique. Office International des Epizooties (2000), 19(1), 64-78

Hantaviruses are the causative agents of the zoonotic diseases known as haemorrhagic fever with renal syndrome (HFRS) in Europe and Asia, and hantavirus pulmonary syndrome (HPS) in the Americas. These ... [more ▼]

Hantaviruses are the causative agents of the zoonotic diseases known as haemorrhagic fever with renal syndrome (HFRS) in Europe and Asia, and hantavirus pulmonary syndrome (HPS) in the Americas. These pathogens are maintained in the wild by rodent reservoirs and are mainly transmitted via the aerosol route. The infection is chronic and apparently asymptomatic in host animals. Whilst HFRS is caused by Hantaan, Seoul, Dobrava and Puumala hantaviruses, HPS is associated with Sin Nombre-like viruses. Common clinical features of HFRS and HPS include fever, myalgia, thrombocytopenia, leukocytosis and a capillary leak syndrome associated with shock in most severe cases. Outbreaks of HFRS and HPS are generally observed during years with dense rodent populations resulting from favourable climatic and environmental conditions. Human activities, such as rodent trapping, farming, cleaning rodent-infested areas, construction work, camping and hunting, are also implicated in the occurrence of hantavirus disease. Prophylactic measures in endemic areas rely essentially on information campaigns and rodent control. [less ▲]

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See detailSpatial and Temporal Dynamics of Puumala Hantavirus Infection in Red Bank Vole (Clethrionomys Glareolus) Populations in Belgium
Escutenaire, Sophie; Chalon, Patrice ULiege; Verhagen, Ron et al

in Virus Research (2000), 67(1), 91-107

Dynamics of hantavirus infection and population densities in rodents were investigated from 1996 to 1999 in southern Belgium. Evidence of Puumala infection was restricted to Clethrionomys glareolus ... [more ▼]

Dynamics of hantavirus infection and population densities in rodents were investigated from 1996 to 1999 in southern Belgium. Evidence of Puumala infection was restricted to Clethrionomys glareolus. Although the serotype was not determined, antibodies against hantavirus were also found in eight Apodemus sylvaticus. In fall 1996, the seroprevalence in C. glareolus was high (20.1%, 37 of 184) and the infection was widely distributed in the area studied whereas a focal occurrence of positive rodents and lower seroprevalence rates were recorded in spring 1997 (14.3%, six of 42), fall 1997 (6. 6%, 11 of 166), spring 1998 (6.4%, three of 47) and fall 1998 (6.7%, 11 of 165). A pullulation of rodents was observed in spring 1999 and was associated with a markedly higher seroprevalence in C. glareolus (47.7%, 189 of 396). In all seasons, infection rates in adults were higher than in juveniles and subadults. No significant difference of prevalence was recorded between males and females. In two trapping sites, the temporary disappearance of positive animals after a crash in rodent populations suggests that a threshold in density is necessary for the maintenance of the enzootic cycle. [less ▲]

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See detailDeux ans d'absence de rage chez le renard en Belgique. Bilan de l'épidémiosurveillance de la rage en 1999
Brochier, Bernard; Dechamps, Pierre; Costy, Françoise et al

in Annales de Médecine Vétérinaire (2000), 144(4), 247-254

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See detailL'herpèsvirus bovin de type 4 : virus pathogène ou passager?
Thiry, Etienne ULiege; Markine-Goriaynoff, Nicolas ULiege; Minner, Frédéric et al

in Point Vétérinaire (2000), 31

Bovine herpesvirus type 4 (BHV-4) is a ubiquitous herpesvirus in cattle. It has been isolated from animals showing a wide variety of clinical signs but few of the isolates have proven experimental ... [more ▼]

Bovine herpesvirus type 4 (BHV-4) is a ubiquitous herpesvirus in cattle. It has been isolated from animals showing a wide variety of clinical signs but few of the isolates have proven experimental pathogenicity. Species that are susceptible to BHV-4 include animals other than ruminants, notably cats and, surprisingly, a primate the owl monkey (Aotus trivirgatus). BHV-4 exists as a latent infection in mononuclear cells. In cattle, BHV-4 has been isolated in animals exhibiting ocular and respiratory conditions and it has been found in females with genital tract conditions such as post-partum metritis and vulvovaginitis. There is some epidemiological and experimental evidence that BHV-4 may be a cause of bovine abortion. There is no vaccine available in Europe and prevention is exclusively by hygiene measures. [less ▲]

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See detailAn approach to the control of disease transmission in pig-to-human xenotransplantation.
Onions, David E.; Cooper, David K.C.; Alexander, Tom J.L. et al

in Xenotransplantation (2000), 7(2), 143-55

Although several major immunologic hurdles need to be overcome, the pig is currently considered the most likely source animal of cells, tissues and organs for transplantation into humans. Concerns have ... [more ▼]

Although several major immunologic hurdles need to be overcome, the pig is currently considered the most likely source animal of cells, tissues and organs for transplantation into humans. Concerns have been raised with regard to the potential for the transfer of infectious agents with the transplanted organ to the human recipient. This risk is perceived to be increased as it is likely that the patient will be iatrogenically immunocompromised and the organ-source pig may be genetically engineered in such a way to render its organs particularly susceptible to infection with human viruses. Furthermore, the risk may not be restricted to the recipient, but may have consequences for the health of others in the community. The identification of porcine endogenous retroviruses and of hitherto unknown viruses have given rise to the most concern. We document here the agents we believe should be excluded from the organ-source pigs. We discuss the likelihood of achieving this aim and outline the potential means by which it may best be achieved. [less ▲]

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See detailEpidemiologische bewaking van boviene spongiforme encefalopathie in Belgïe: Jaaroverzicht voor 1998
Saegerman, Claude ULiege; Dechamps, P.; Vanopdenbosch, E. et al

in Vlaams Diergeneeskundig Tijdschrift (2000), 69

In 1998 werden 6 runderen tussen de 54 en 71 maanden ouderdom, afkomstig van de provincies West-Vlaanderen (3 gevallen), Oost-Vlaanderen (2 gevallen) en Luik (1 geval), gediagnostiseerd als gevallen van ... [more ▼]

In 1998 werden 6 runderen tussen de 54 en 71 maanden ouderdom, afkomstig van de provincies West-Vlaanderen (3 gevallen), Oost-Vlaanderen (2 gevallen) en Luik (1 geval), gediagnostiseerd als gevallen van boviene spongiforme encephalopathie (BSE). De hypotheses betreffende de oorsprong van de infectie op zijn de volgende : het optreden van sporadische gevallen zonder duidelijk definieerbare oorzaak; de mogelijke kruiscontaminatie tussen voeder voor monogastrische dieren met daarin dierlijk meel en voeder voor herkauwers waarin geen dierlijk meel is verwerkt en dit tijdens het fabricatieproces, de stockage, het transport of de distributie; het gebruik van dierlijk beendermeel in het voeder voor runderen geproduceerd voor de ban (van kracht vanaf 27/7/1994). Algemeen kan men dus stellen dat in België de aanwezigheid van gecontamineerd diermeel als risicofactor voor BSE niet kan worden uitgesloten. De oorsprong van deze diermelen kon nog niet gedetermineerd worden. [less ▲]

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See detailEssai clinique d'innocuité et d'efficacité d'un vaccin antirabique inactivé et purifié à usage humain
Costy, F.; Renders, C.; Brochier, B. et al

in Annales de Médecine Vétérinaire (2000), 145(2), 65-72

Quatre cent soixante etudiants veterinaires en debut d'essai clinique (220 en Belgique et 240 en France) ont recu une primovaccination par voie intramusculaire (JO, J7 et J28) ainsi qu'un rappel apres 1 ... [more ▼]

Quatre cent soixante etudiants veterinaires en debut d'essai clinique (220 en Belgique et 240 en France) ont recu une primovaccination par voie intramusculaire (JO, J7 et J28) ainsi qu'un rappel apres 1 an avec trois lots industriels successifs d'un vaccin antirabique prepare sur cellules Vero et purifie par chromato­ graphie . Les resultats immunologiques tant apres primovaccination (J42) qu'apres rappel (J379) etaient supe­rieurs au seuil de protection theoriquement admis de 0,5Ul/ml. On n'a pas mis en evidence de difference si­gnificative entre les trois lots de vaccin utilises en terme de moyenne geometrique des titres en anticorps antirabiques neutralisants. Quant a l'innocuite de ce vaccin, les reactions locales et systemiques etaient en ge­neral legeres. Aucune reaction immediate severe n'a ete observee et aucun evenement indesirable grave n'a ete relie au vaccin. Les reactions locales sont le plus souvent des douleurs a l'endroit de !'injection: elles ap­paraissent dans 60% des cas en primovaccination et 53% des cas en rappel. Ces douleurs apparaissent le plus souvent dans les 24 heures apres vaccination et durent generalement 24 a 48 heures. L'incidence des autres reactions locales ne depasse jamais 9,8%. Les reactions systemiques sont le plus souvent legeres ou moderees; on y retrouve le plus souvent des troubles gastro-intestinaux et generaux. Le pourcentage de. su­ jets presentant au moins une reaction systemique variait de plus ou moins 20% en primovaccination a plus ou moins 15% lors du rappel. On n'a pas mis en evidence de difference significative dans la frequence d'appari­ tion des reactions locales et generales en fonction du lot, et cela quelle que soit la dose rec;ue. On peut conclure de maniere generale que la vaccination avec le vaccin antirabique prepare sur cellules Vero et purifie par chro­ matographie s'est revelee efficace et bien toleree. [less ▲]

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See detailLes molécules orphelines en médecine vétérinaire
Urbain, B.; Laurier, L.; Falize, Françoise et al

in Annales de Médecine Vétérinaire (2000), 144(1), 5-11

La disponibilité du médicament à usage vétérinaire dans l'Union européenne est devenue un problème crucial dans le domaine de la thérapeutique des animaux de rente étant donné qu'au 1ier janvier 2000 ... [more ▼]

La disponibilité du médicament à usage vétérinaire dans l'Union européenne est devenue un problème crucial dans le domaine de la thérapeutique des animaux de rente étant donné qu'au 1ier janvier 2000, nombre de substances ont été condamnées à disparaître du marché. Les substances indispensables, dénommées molécules orphelines, font actuellement l'objet d'un débat entre les parties intéressées par le problème de la disponibilité du médicament. Cet article tente d'expliquer les causes responsables de cette situation et de présenter les solutions proposées au niveau de l'Union européenne afin d'améliorer la disponibilité du médicament en médecine vétérinaire. Le cas particulier de la Belgique est abordé. [less ▲]

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See detailPurification and characterisation of bovine wc1+ γδ T lymphocytes from peripheral blood
Fikri, Youssef; Nyabenda, Jean; Denis, Martine et al

in Veterinary Research (2000), 31(2), 229-239

In order to isolate and characterise resting WCl+ γδT cells from cattle, we developed a protocol for purifying these cells by negative selection from peripheral blood. The purification method included ... [more ▼]

In order to isolate and characterise resting WCl+ γδT cells from cattle, we developed a protocol for purifying these cells by negative selection from peripheral blood. The purification method included five steps: separation of mononuclear cells on lymphoprep, depletion of monocytes by adherence to plasma-coated gelatin enriching T cells on a nylon wool column, depleting CD2+ T cells by sheep red blood cells (SRBC), and finally depleting CD4+ and CD8+ T cells by the magnetic cell sorting technique (MACS). This procedure proved efficient and reproducible, and the purity of the isolated WC1+ γδT cells was more than 97% as analysed by flow cytometry (FACS). Cytokines and costimulatory molecules mRNA expression was assessed by the reverse transcriptase polymerase chain reaction (RT-PCR) technique in freshly isolated resting WC1+ T cells. We found that purified uncultured WC1+ T cells express TNF-α, CD28, CTLA-4 and IL-2Rα mRNA transcripts but do not express those for IL-2, IL-4, IL-6, IL-10 and IFN-γ. The expression of CD28 and CTLA-4 transcripts on bovine WC1+ T cells indicates that these genes are evolutionarily conserved. [less ▲]

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See detailL'Université de Liège accueille le deuxième congrès international de vaccinologie
Pastoret, Paul-Pierre ULiege

in Annales de Médecine Vétérinaire (2000), 144(6), 427-429

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See detailVeterinary Vaccinology
Pastoret, Paul-Pierre ULiege

in Comptes Rendus de l'Académie des Sciences. Série III. Sciences de la Vie (1999), 322(11), 967-72

Veterinary vaccinology is a very interesting and rapidly developing field. In fact veterinary vaccines are not only used for the prevention of infectious diseases in the animal health sector, but also ... [more ▼]

Veterinary vaccinology is a very interesting and rapidly developing field. In fact veterinary vaccines are not only used for the prevention of infectious diseases in the animal health sector, but also help to solve problems of public health, to reduce detrimental environmental impact of the use of some veterinary drugs and prevent the emergence of resistance of micro-organisms or parasites. After a short introduction, this paper will deal with the use of vaccines for animal health and welfare, including new developments in the veterinary field such as marker vaccines and vectored vaccines, the special case of equine influenza-inactivated vaccines and the use of veterinary vaccines in public health. The conclusions will analyse the reasons as to why develop veterinary vaccines and the obstacles to their development. [less ▲]

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