References of "Guiot, Julien"
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See detailMacrophage-derived exosomes attenuate fibrosis in airway epithelial cells through delivery of antifibrotic miR-142-3p
GUIOT, Julien ULiege; Cambier, Maureen ULiege; Boeckx, Amandine ULiege et al

in Thorax (2020)

Introduction: Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown aetiology and cure. Recent studies have reported a dysregulation of exosomal microRNAs ... [more ▼]

Introduction: Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown aetiology and cure. Recent studies have reported a dysregulation of exosomal microRNAs (miRs) in the IPF context. However, the impact of IPF-related exosomal miRs on the progression of pulmonary fibrosis is unknown. Methods: Two independent cohorts were enrolled at the ambulatory care polyclinic of Liège University. Exosomes from sputum were obtained from 19 patients with IPF and 23 healthy subjects (HSs) (cohort 1), and the ones from plasma derived from 14 patients with IPF and 14 HSs (cohort 2). Exosomal miR expression was performed by quantitative reverse transcription–PCR. The functional role of exosomal miRs was assessed in vitro by transfecting miR mimics in human alveolar epithelial cells and lung fibroblasts. Results: Exosomal miR analysis showed that miR-142-3p was significantly upregulated in sputum and plasma of patients with IPF (8.06-fold, p<0.0001; 1.64 fold, p=0.008, respectively). Correlation analysis revealed a positive association between exosomal miR-142-3p and the percentage of macrophages from sputum of patients with IPF (r=0.576, p=0.012), suggesting macrophage origin of exosomal miR-142-3p upregulation. The overexpression of miR-142-3p in alveolar epithelial cells and lung fibroblasts was able to reduce the expression of transforming growth factor β receptor 1 (TGFβ-R1) and profibrotic genes. Furthermore, exosomes isolated from macrophages present antifibrotic properties due in part to the repression of TGFβ-R1 by miR-142-3p transfer in target cells. Discussion: Our results suggest that macrophage-derived exosomes may fight against pulmonary fibrosis progression via the delivery of antifibrotic miR-142–3 p to alveolar epithelial cells and lung fibroblasts. [less ▲]

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See detailExosomal Long Non-Coding RNAs in Lung Diseases
Poulet, Christophe ULiege; NJOCK, Makon-Sébastien ULiege; MOERMANS, Catherine ULiege et al

in International Journal of Molecular Sciences (2020), 21(10), 3580

Within the non-coding genome landscape, long non-coding RNAs (lncRNAs) and their secretion within exosomes are a window that could further explain the regulation, the sustaining, and the spread of lung ... [more ▼]

Within the non-coding genome landscape, long non-coding RNAs (lncRNAs) and their secretion within exosomes are a window that could further explain the regulation, the sustaining, and the spread of lung diseases. We present here a compilation of the current knowledge on lncRNAs commonly found in Chronic Obstructive Pulmonary Disease (COPD), asthma, Idiopathic Pulmonary Fibrosis (IPF), or lung cancers. We built interaction networks describing the mechanisms of action for COPD, asthma, and IPF, as well as private networks for H19, MALAT1, MEG3, FENDRR, CDKN2B-AS1, TUG1, HOTAIR, and GAS5 lncRNAs in lung cancers. We identified five signaling pathways targeted by these eight lncRNAs over the lung diseases mentioned above. These lncRNAs were involved in ten treatment resistances in lung cancers, with HOTAIR being itself described in seven resistances. Besides, five of them were previously described as promising biomarkers for the diagnosis and prognosis of asthma, COPD, and lung cancers. Additionally, we describe the exosomal-based studies on H19, MALAT1, HOTAIR, GAS5, UCA1, lnc-MMP2-2, GAPLINC, TBILA, AGAP2-AS1, and SOX2-OT. This review concludes on the need for additional studies describing the lncRNA mechanisms of action and confirming their potential as biomarkers, as well as their involvement in resistance to treatment, especially in non-cancerous lung diseases. [less ▲]

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See detailDevelopment of a Clinical Decision Support System for Severity Risk Prediction and Triage of COVID-19 Patients at Hospital Admission: an International Multicenter Study.
Wu, Guangyao; Yang, Pei; Xie, Yuanliang et al

in European Respiratory Journal (2020)

BACKGROUND: The outbreak of the coronavirus disease 2019 (COVID-19) has globally strained medical resources and caused significant mortality. OBJECTIVE: To develop and validate machine-learning model ... [more ▼]

BACKGROUND: The outbreak of the coronavirus disease 2019 (COVID-19) has globally strained medical resources and caused significant mortality. OBJECTIVE: To develop and validate machine-learning model based on clinical features for severity risk assessment and triage for COVID-19 patients at hospital admission. METHOD: 725 patients were used to train and validate the model including a retrospective cohort of 299 hospitalised COVID-19 patients at Wuhan, China, from December 23, 2019, to February 13, 2020, and five cohorts with 426 patients from eight centers in China, Italy, and Belgium, from February 20, 2020, to March 21, 2020. The main outcome was the onset of severe or critical illness during hospitalisation. Model performances were quantified using the area under the receiver operating characteristic curve (AUC) and metrics derived from the confusion-matrix. RESULTS: The median age was 50.0 years and 137 (45.8%) were men in the retrospective cohort. The median age was 62.0 years and 236 (55.4%) were men in five cohorts. The model was prospectively validated on five cohorts yielding AUCs ranging from 0.84 to 0.89, with accuracies ranging from 74.4% to 87.5%, sensitivities ranging from 75.0% to 96.9%, and specificities ranging from 57.5% to 88.0%, all of which performed better than the pneumonia severity index. The cut-off values of the low, medium, and high-risk probabilities were 0.21 and 0.80. The online-calculators can be found at www.covid19risk.ai. CONCLUSION: The machine-learning model, nomogram, and online-calculator might be useful to access the onset of severe and critical illness among COVID-19 patients and triage at hospital admission. [less ▲]

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See detailCOVID-19 dual energy CT scan for a double sided disease
DEPREZ, Louis ULiege; LOVINFOSSE, Pierre ULiege; Boulanger, Yves-Gauthier et al

in Thorax (2020)

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See detailDevelopment and validation of an automated radiomic CT signature for detecting COVID-19
Vaidyantahan, Akshayaa; GUIOT, Julien ULiege; DEPREZ, Louis ULiege et al

in European Respiratory Journal (2020)

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See detailSuitable reference genes determination for real-time PCR using induced sputum samples.
MOERMANS, Catherine ULiege; Deliege, Esteban; Pirottin, Dimitri ULiege et al

in European Respiratory Journal (2019), 54(6),

Induced sputum is a non-invasive method of collecting cells from airways. Gene expression analysis from sputum cells has been used to understand the underlying mechanisms of airway diseases such as asthma ... [more ▼]

Induced sputum is a non-invasive method of collecting cells from airways. Gene expression analysis from sputum cells has been used to understand the underlying mechanisms of airway diseases such as asthma or chronic obstructive pulmonary disease (COPD). Suitable reference genes for normalisation of target mRNA levels between sputum samples have not been defined so far.The current study assessed the expression stability of nine common reference genes in sputum samples from 14 healthy volunteers, 12 asthmatics and 12 COPD patients.Using three different algorithms (geNorm, NormFinder and BestKeeper), we identified HPRT1 and GNB2L1 as the most optimal reference genes to use for normalisation of quantitative reverse transcriptase (RT) PCR data from sputum cells. The higher expression stability of HPRT1 and GNB2L1 were confirmed in a validation set of patients including nine healthy controls, five COPD patients and five asthmatic patients. In this group, the RNA extraction and RT-PCR methods differed, which attested that these genes remained the most reliable whatever the method used to extract the RNA, generate complementary DNA or amplify it.Finally, an example of relative quantification of gene expression linked to eosinophils or neutrophils provided more accurate results after normalisation with the reference genes identified as the most stable compared to the least stable and confirmed our findings. [less ▲]

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See detailLe syndrome de détresse respiratoire aiguë
Parzibut, Gilles ULiege; CANIVET, Jean-Luc ULiege; GUIOT, Julien ULiege et al

in Revue Médicale de Liège (2019), 74(10), 514-520

Since its first description in 1967, a lot of progress has been made in understanding the pathophysiology, diagnosis and management of acute respiratory distress syndrome (ARDS). This nosological entity ... [more ▼]

Since its first description in 1967, a lot of progress has been made in understanding the pathophysiology, diagnosis and management of acute respiratory distress syndrome (ARDS). This nosological entity is based on the appearance of a diffuse alveolar damage associating pulmonary epithelial barrier disruption with an alveolar filling, both responsible of profound hypoxemia and important morbi-mortality. Nowadays, ARDS remains a frequent syndrome, associated with various etiologies. Diagnosis is based on the occurrence of acute hypoxic respiratory failure not explained by cardiac insufficiency or volume overload, within 7 days after a recognized risk factor, and in the presence of bilateral pulmonary opacities not fully explained by effusions, atelectasis or nodules on the chest radiography. Survivors present an increased risk of developing cognitive decline, depression, post-traumatic stress, and typical ICU related side-effects such as polyneuropathy and sarcopenia. In this context and not withstanding significant recent progress in the field of mechanical ventilation and extra-corporeal respiratory assistance, early diagnosis remains essential to identify patients with ARDS in order to offer them the most appropriate therapy. [less ▲]

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See detailIgG4-related pleural disease in a patient with a history of unknown origin acute pancreatitis: a case report and review of the literature.
Damas, François ULiege; Ghysen, K.; GESTER, Fanny ULiege et al

in Acta Clinica Belgica (2019), 8

Immunoglobulin G4-related disease is a rare autoimmune systemic disease with the capability of involving every organ. The disease is microscopically defined by a diffuse tissular inflammation with an ... [more ▼]

Immunoglobulin G4-related disease is a rare autoimmune systemic disease with the capability of involving every organ. The disease is microscopically defined by a diffuse tissular inflammation with an infiltration of IgG4 positive plasma cells in the affected organs. IgG4 disease has an increasing incidence in the last few years with a growing interest in its pathophysiology still misunderstood to date. Despite the growing recognition of this pathology, the literature still does not allow to propose a simple diagnostic algorithm. In this article, we present a case of a 56-year-old man with a history of unknown etiology acute pancreatitis and a unilateral pleural effusion. [less ▲]

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See detailExosomal miRNAs in Lung Diseases: From Biologic Function to Therapeutic Targets.
GUIOT, Julien ULiege; Struman, Ingrid ULiege; Louis, Edouard ULiege et al

in Journal of clinical medicine (2019), 8(9),

Increasing evidence suggests the potential role of extracellular vesicles (EVs) in many lung diseases. According to their subcellular origin, secretion mechanism, and size, EVs are currently classified ... [more ▼]

Increasing evidence suggests the potential role of extracellular vesicles (EVs) in many lung diseases. According to their subcellular origin, secretion mechanism, and size, EVs are currently classified into three subpopulations: exosomes, microvesicles, and apoptotic bodies. Exosomes are released in most biofluids, including airway fluids, and play a key role in intercellular communication via the delivery of their cargo (e.g., microRNAs (miRNAs)) to target cell. In a physiological context, lung exosomes present protective effects against stress signals which allow them to participate in the maintenance of lung homeostasis. The presence of air pollution alters the composition of lung exosomes (dysregulation of exosomal miRNAs) and their homeostatic property. Indeed, besides their potential as diagnostic biomarkers for lung diseases, lung exosomes are functional units capable of dysregulating numerous pathophysiological processes (including inflammation or fibrosis), resulting in the promotion of lung disease progression. Here, we review recent studies on the known and potential role of lung exosomes/exosomal miRNAs, in the maintaining of lung homeostasis on one hand, and in promoting lung disease progression on the other. We will also discuss using exosomes as prognostic/diagnostic biomarkers as well as therapeutic tools for lung diseases. [less ▲]

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See detailComment j'explore... Les modifications biologiques dans les pathologies infiltrantes pulmonaires
GESTER, Fanny ULiege; DUYSINX, Bernard ULiege; VON FRENCKELL, Christian ULiege et al

in Revue medicale de Liege (2019), 74(1), 47-53

Interstitial lung diseases (ILD) are a part of a vast and heterogeneous clinicopathological entity. The work-up have to rule out a granulomatosis or a secondary cause, before making the diagnosis of an ... [more ▼]

Interstitial lung diseases (ILD) are a part of a vast and heterogeneous clinicopathological entity. The work-up have to rule out a granulomatosis or a secondary cause, before making the diagnosis of an idiopathic ILD. The etiological diagnosis is based on a multidisciplinary approach integrating a network of clinical and paraclinical datas. If the diagnosis remains unclear, a lung biopsy is suggested with a transbronchial approach (mainly cryobiopsy) or with a surgical approach (video-assisted thoracoscopy). This review article mainly describes the biological analyses that contribute to explore ILDs. [less ▲]

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See detailBiomarkers in systemic sclerosis-associated interstitial lung disease: review of the literature.
BONHOMME, Olivier ULiege; ANDRE, Béatrice ULiege; GESTER, Fanny ULiege et al

in Rheumatology (Oxford, England) (2019)

SSc is a rare disease of unknown origin associated with multiple organ involvement. One of the major complications that drives the mortality of SSc patients is interstitial lung disease. The course of SSc ... [more ▼]

SSc is a rare disease of unknown origin associated with multiple organ involvement. One of the major complications that drives the mortality of SSc patients is interstitial lung disease. The course of SSc-interstitial lung disease progression has a wide spectrum. Since the treatment is based on aggressive immunosuppression it should not be given to stable or non-progressing disease. The correct identification of disease with high risk of progression remains a challenge for early therapeutic intervention, and biomarkers remain urgently needed. In fact, eight categories of biomarkers have been identified and classified according to the different biological pathways involved. The purpose of this article is to describe the main biomarkers thought to be of interest with clinical value in the diagnosis and prognosis of SSc-interstitial lung disease. [less ▲]

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See detailL'hypertension artérielle pulmonaire
GUIOT, Julien ULiege; Parzibut, Gilles ULiege; Weber, T. et al

in Revue Médicale de Liège (2019), 74(3), 139-145

Pulmonary arterial hypertension (PAH) is a rare vascular lung disease with a complex etiopathogeny characterized by an increased pulmonary arterial pressure of 25 mmHg or above assessed by right heart ... [more ▼]

Pulmonary arterial hypertension (PAH) is a rare vascular lung disease with a complex etiopathogeny characterized by an increased pulmonary arterial pressure of 25 mmHg or above assessed by right heart catheterization. The diagnosis is difficult due to the atypical presentation with shortness of breath requiring a sequential approach bringing at the end the clinician to perform a right heart catheterization. Nowadays, several therapies have proven to be efficient for treating PAH. Recently, international recommendations have moved to an initial combination therapy reducing the overall morbi-mortality of the patients. Therefore, early therapy appears to be a priority in PAH underlying the need for increasing the global knowledge around PAH. © 2019 Revue Medicale de Liege. All Rights Reserved. [less ▲]

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See detailInsulin-Only STAR: Liège Clinical Trial Interim Results on Safety and Efficacy
Uyttendaele, Vincent ULiege; Knopp, Jennifer L.; PIROTTE, Marc ULiege et al

Poster (2018, November)

Objective: Stress-induced hyperglycaemia and insulin resistance is common in critically ill patients and associated with worsened outcomes. STAR (Stochastic TARgeted) glycaemic control (GC) has proven ... [more ▼]

Objective: Stress-induced hyperglycaemia and insulin resistance is common in critically ill patients and associated with worsened outcomes. STAR (Stochastic TARgeted) glycaemic control (GC) has proven effective over different units and clinical practices. However, this risk-based dosing approach uses both insulin and nutrition to control glycemia, where virtually all others use insulin only. This study uses STAR with insulin only and nutrition set clinically in the University Hospital of Liège, Belgium, to assess safety and efficacy in this use. Method: STAR-Liège is an insulin-only version of STAR targeting 80-145mg/dL. Patient are included if two successive BG measurements are >145mg/dL. Insulin is administered through IV catheter continuously and nutrition clinically set. GC was stopped after 72h or if BG was stable at insulin rate ≤2U/h. Safety is assessed by the %BG in severe (<40mg/dL), mild (<72mg/dL) hypoglycaemia, and >180mg/dL. Performance is evaluated by the %BG within target band and median BG. Clinical data from the first 11 patients is analysed, totalling 645 hours of control. Ethics approval was granted by the University Hospital of Liège Ethics Committee. Results: The insulin-only STAR-Liège protocol showed high performance, with median [IQR] BG of 122 [106, 147] mg/dL and 78% BG in target band. Mild hypoglycaemia occurred 1.6% of time, but there was no incidence of severe hypoglycaemia. Additionally, only 9.8% BG>180mg/dL, and administered insulin and nutrition was 4.0 [1.8, 4.6] U/h and 8.1 [4.9, 9.2] g/h. Conclusions: Insulin-only GC with the STAR-Liège protocol provided equally high control safety and quality for all patients. These results are encouraging, comparable to previous studies, and supporting STAR risk-based dosing approach as a robust solution across different ICU settings and usages, and support continuation of the clinical trial. [less ▲]

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See detailSputum exosomes - promising biomarkers for idiopathic pulmonary fibrosis
Njock, Makon-Sébastien ULiege; GUIOT, Julien ULiege; HENKET, Monique ULiege et al

Poster (2018, May)

Background: Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown etiology which leads rapidly to death. As diagnosis of IPF is complex, the development of ... [more ▼]

Background: Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown etiology which leads rapidly to death. As diagnosis of IPF is complex, the development of novel molecular biomarkers is a central challenge for the future of translational research. Consequently, we sought to characterize microRNA (miR) content of exosomes from sputum of IPF patients compared to healthy donors in order to identify novel biomarkers of the disease. Methods: Exosomes were isolated from induced sputum samples of 14 IPF patients diagnosed following American Thoracic Society (ATS) /European Respiratory Society (ERS) recommendations and 11 healthy donors with standard ultracentrifugation protocol. Exosomal miR content was analysed by miR qPCR arrays, and diseases/biological processes associated to altered miRs were determined by bioinformatic analysis. Results: The presence of exosomes was confirmed in sputum from both IPF patients and healthy donors. The profiling of exosomal miRs revealed 21 differentially expressed miRs in the sputum of IPF patients compared to healthy donors. Further validation of miRs presenting an aberrant expression allowed us to identify for the first time an IPF-specific miR signature from sputum exosomes, among which miR-142-3p and miR-33a-5p present an upregulation (fold change (FC)>3, p < 0.01), whereas let-7d-5p a downregulation (FC < 0.5, p < 0.01). The bioinformatic analysis revealed that altered miRs are associated to inflammatory diseases, among which IPF is the most relevant one (p = 3.78E-10). Interestingly, most of the biological processes highlighted in this analysis are in agreement with IPF etiology, which confers to our candidates an evident role as IPF biomarkers. Based on these findings, functional tests with IPF-sputum exosomes and mimics of altered miRs are underway to test their impact on IPF progression. Summary/Conclusion: For the first time, we identified potential biomarkers for IPF from sputum exosomes. Our findings may thus lead to a better understanding about the roles of these miRs in the pathogenesis of IPF and thus open new avenues for therapeutic approaches. This study reinforced the concept that sputum exosomes might be a novel source of biomarkers for the diagnosis of pulmonary diseases. [less ▲]

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See detailDétresse respiratoire aiguë révélatrice d'un syndrome des antisynthétases
Maloir, Quentin ULiege; Ghysen, Katrien ULiege; VON FRENCKELL, Christian ULiege et al

in Revue Médicale de Liège (2018), 73(7-8), 370-375

Antisynthetase syndrome is a clinical entity characterized by specific anti-aminoacyl-tRNA-synthetase antibodies usually associated with inflammatory myopa-thy and interstitial lung disease. The classic ... [more ▼]

Antisynthetase syndrome is a clinical entity characterized by specific anti-aminoacyl-tRNA-synthetase antibodies usually associated with inflammatory myopa-thy and interstitial lung disease. The classic presentation of the pathology is the pulmonary interstitium involvment, wich commonly determines the global prognosis. The subsequent diagnosis of antisynthetase syndrome in patients with acute respiratory distress syndrome (ARDS) is unusual, even more so when a veino-veinous (VV) extracorporeal membrane oxygenation (ECMO) is required. This article presents a clinical case of antisynthetase syndrome with severe ARDS successfully treated with immunosup-pressive agents and ECMO. © 2018 Revue Medicale de Liege. All rights reserved. [less ▲]

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See detailLe medicament du mois Trimbow(R) ou triple association bronchodilatatrice et anti-inflammatoire en aérosol dans la broncho-pneumopathie chronique obstructive
Corhay, Jean-Louis ULiege; BONHOMME, Olivier ULiege; GUIOT, Julien ULiege

in Revue Médicale de Liège (2018), 73(9), 480-484

Single-inhaler triple therapy in extrafine solution combining an inhaled corticosteroid (ICS), the dipropionate of beclometasone, a long acting ss2-agonist (LABA), the fumarate of formoterol and an long ... [more ▼]

Single-inhaler triple therapy in extrafine solution combining an inhaled corticosteroid (ICS), the dipropionate of beclometasone, a long acting ss2-agonist (LABA), the fumarate of formoterol and an long-acting muscarinic antagonist (LAMA), the bromide of glycopyrronium, was developed for the treatment of the chronic obstructive pulmonary disease (COPD). Trimbow(R) is the first triple therapy in spray with fixed dose and containing 3 pharmacological agents (LABA-LAMA-ICS). Clinical trials show that Trimbow(R) improves numerous parameters such as the respiratory function, the quality of life, the symptoms and the rate of moderate to severe exacerbations while being tolerated well. These results justify its use in severe and very severe COPD with exacerbations in spite of treatment by LABA-LAMA or LABA-ICS. In this article, we present a brief synthesis of the main recent clinical trials on Trimbow(R), its comparison with other pharmacological agents/associations regularly used in the treatment of COPD, as well as some practical information on its use in routine. [less ▲]

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See detailSputum exosomes: promising biomarkers for idiopathic pulmonary fibrosis.
Njock, Makon-Sébastien ULiege; GUIOT, Julien ULiege; HENKET, Monique ULiege et al

in Thorax (2018)

Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown aetiology which leads rapidly to death. As diagnosis of IPF is complex, we aimed to characterise ... [more ▼]

Idiopathic pulmonary fibrosis (IPF) is a progressive fibrosing interstitial lung disease of unknown aetiology which leads rapidly to death. As diagnosis of IPF is complex, we aimed to characterise microRNA (miRNA) content of exosomes from sputum of patients with IPF. Using miRNA quantitative PCR array, we found a substantial dysregulation of sputum exosomal miRNA levels between patients with IPF and healthy subjects and identified a unique signature of three miRNAs. Interestingly, we found a negative correlation between miR-142-3p and diffusing capacity of the lungs for carbon monoxide/alveolar volume. This is the first characterisation of miRNA content of sputum-derived exosomes in IPF that identified promising biomarkers for diagnosis and disease severity. [less ▲]

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See detailPreliminary results from the STAR-Liège clinical trial: Virtual trials, safety, performance, and compliance analysis
Uyttendaele, Vincent ULiege; Knopp, Jennifer L.; PIROTTE, Marc ULiege et al

in IFAC-PapersOnLine (2018)

Glycaemic control has been shown to improve outcome in critically ill patients, but hard to achieve in a safe and effective manner. This paper presents the preliminary results of 8 patients controlled at ... [more ▼]

Glycaemic control has been shown to improve outcome in critically ill patients, but hard to achieve in a safe and effective manner. This paper presents the preliminary results of 8 patients controlled at the University Hospital of Liège under STAR-Liège, an insulin-only version of the model-based STAR glycaemic controller framework. Clinical data is compared with virtual trial simulations of the glycaemic control outcomes for the STAR-Liège protocol, and with the standard of care protocol of this intensive care unit, to assess safety, performance, and compliance of the new protocol. Results show 78% of clinical blood glucose measurements in target band. Only 3% of blood glucose measurements were below 4.4 mmol/L (79 mg/dL), with only 1% mild hypoglycaemia and no severe hypoglycaemia. These results are similar to simulation of the protocol, but slightly higher workload is observed clinically due to nursing choice. Compared to standard protocol virtual trial simulations, STAR-Liège achieved tighter and less variable control with similar safety, and less percentage time in higher blood glucose levels. Clinically, 14% of insulin intervention were increased or decreased from recommendation with median [IQR] change of 1 [1, 2] or -2 [-3, -2] U/hr respectively. Clinical and simulation results show STAR-Liège better controls glycaemia to lower ranges compared to the standard protocol, while ensuring safety. Lower time in higher blood glucose ranges potentially improves patient outcomes. Compliance analysis shows potential nurse fears in protocol changes and different insulin dosing. These results are encouraging for the continuation of the clinical trial realised in this medical intensive care unit and its extension to insulin and nutrition control. [less ▲]

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See detailComment j'explore... Techniques diagnostiques invasives des pneumopathies interstitielles diffuses
DUYSINX, Bernard ULiege; GUIOT, Julien ULiege; PELLEGRINI, Ivan ULiege et al

in Revue Médicale de Liège (2018), 73(3), 147-155

Interstitial lung diseases represent a very heterogeneous group of diseases mainly affecting connective lung tissue even if alveolar space may sometimes be involved. The identification of their etiology ... [more ▼]

Interstitial lung diseases represent a very heterogeneous group of diseases mainly affecting connective lung tissue even if alveolar space may sometimes be involved. The identification of their etiology is the key stage in their management. It requires the integration of anamnestic, clinical, biological, radiological data and, sometimes relies on, cytology or histology. In this review, we assess the contribution and feasibility of the different invasive techniques used for interstitial lung disease diagnosis. In particular we focus on the yield of lung endoscopy in casting light on the multidisciplinary confrontation, which is the gold standard of the interstitial lung disease care management. [less ▲]

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See detailMethodology for Sputum Induction and Laboratory Processing.
GUIOT, Julien ULiege; Demarche, Sophie ULiege; HENKET, Monique ULiege et al

in Journal of Visualized Experiments (2017), (130),

The technique of sputum induction and processing is a recognized non-invasive method allowing the collection and analysis of cells from the airways, which is interesting in various respiratory diseases ... [more ▼]

The technique of sputum induction and processing is a recognized non-invasive method allowing the collection and analysis of cells from the airways, which is interesting in various respiratory diseases like asthma, chronic obstructive pulmonary disease (COPD), chronic cough, or idiopathic pulmonary fibrosis. This technique is well tolerated, safe and non-invasive, but is currently limited to research services and specialized centers in clinical practice because it is technically demanding, time-consuming, and requires trained staff. The success rate of sputum induction and analysis is about 80%. Here, we describe the induction and laboratory processing of sputum samples. Sputum is induced by inhalation of hypertonic or isotonic saline with salbutamol. For the processing, we use the whole sputum technique. Dithiothreitol (DTT) is used to allow mucolysis of sputum samples. The primary aim of sputum processing is to obtain a differential cell count to study the cell types present in the airway lumen. Additional analyses may also be performed on sputum supernatant and sputum cells, which may allow further investigation into inflammatory processes and immune mechanisms. Examples include studying mediators in sputum supernatant and performing a large spectrum of analysis on sputum cells such as flow cytometry, genomics, or proteomics. Finally, representative results of sputum analysis in healthy controls, asthmatics, and COPD patients are presented. [less ▲]

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