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See detailMyopathie atypique et toxicité des samares: réponse de l'expert au courrier des lecteurs
Votion, Dominique ULiege

in Cheval Santé (2020), 126

Réponse via le courrier des lecteurs à diverses questions relatives à la toxicité des érables

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See detailRéponses aux Questions Fréquemment Posées (FAQs) à Propos de l’Alimentation et de la Gestion des Équidés ainsi que de la gestion des Pâtures afin de Réduire le Risque de Myopathie Atypique
François, Anne-Christine ULiege; Votion, Dominique ULiege

in Animals (2020), 10

Cet document est la traduction en français de l'article: Votion D-M., François A-C., Kruse C., Renaud R., Farinelle A., Bouquieaux M-C., Marcillaud-Pitel C., Gustin P. Answers to the frequently asked ... [more ▼]

Cet document est la traduction en français de l'article: Votion D-M., François A-C., Kruse C., Renaud R., Farinelle A., Bouquieaux M-C., Marcillaud-Pitel C., Gustin P. Answers to the frequently asked questions regarding horse feeding and management practices to reduce the risk of atypical myopathy. Il comporte de nombreuses illustrations [less ▲]

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See detailAnswers to the frequently asked questions regarding horse feeding and management practices to reduce the risk of atypical myopathy
Votion, Dominique ULiege; François, Anne-Christine ULiege; Kruse, Caroline ULiege et al

in Animals (2020)

In 2014, atypical myopathy (AM) was linked to Acer pseudoplatanus (sycamore maple) in Europe. The emergence of this seasonal intoxication caused by a native tree has raised many questions. This manuscript ... [more ▼]

In 2014, atypical myopathy (AM) was linked to Acer pseudoplatanus (sycamore maple) in Europe. The emergence of this seasonal intoxication caused by a native tree has raised many questions. This manuscript aims at answering the five most frequently asked questions (FAQs) regarding (1) identification of toxic trees, reduction of risk at the level of (2) pastures and (3) equids, (4) the risk associated with pastures with sycamores that have always been used without horses being poisoned and (5) the length of the risk periods. Answers were found in a literature review and data gathered by AM surveillance networks. A guide is offered FAQ1: a guide is offered to differentiate common maple trees (FAQ1). In order to reduce the risk of AM at pasture levell: (FAQ2): avoid humid pastures, permanent pasturing, spreading of manure and for pasture with sycamores in the vicinity and avoid sycamore maple trees around pasture (FAQ2). To reduce the risk of AM and, at horse level (FAQ3 ): during risk periods, reduce pasturing time according to weather conditions and to less than six hours a day during risk periods for horses on risk pasture, provide supplementary feeds including toxin-free hay, water from the distribution network, vitamins and a salt block (FAQ3). AFAQ4: all pastures with a sycamore tree in the vicinity are at risk (FAQ4). FAQ5: Ninety-four 94% of cases occur over a two 3-month periods, starting in October and in March, for cases resulting from seeds and seedlings ingestion, respectively (FAQ5). [less ▲]

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See detailComment reconnaître les érables de nos régions - Fiche technique
Renaud, Benoît ULiege; François, Anne-Christine ULiege; Dopagne, Claude ULiege et al

Learning material (2019)

Cette fiche reprend les caractéristiques permettant de distinguer les 3 érables communs de nos régions, à savoir: l'érable sycomore, l'érable plane et l'érable champêtre.

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See detailIdentification of the maple tree responsible for atypical myopathy
Renaud, Benoît ULiege; François, Anne-Christine ULiege; Dopagne, Claude ULiege et al

Learning material (2019)

This technical note aims at differentiate the three Acer species commonly found in Europe: Acer pseudoplatanus (sycamore maple), Acer platanoides (Norway maple) and Acer campestre (field maple) .

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See detailIntelligence artificielle et big data: vers une épidémiologie 2.0?
Audic, Mathilde; Gadot, Paul-Marie; Hendrikx, Pascal et al

Conference (2019, November 28)

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See detailDetection of mitochondrial dysfunction by blood mononuclear cells analysis
Kruse, Caroline ULiege; Art, Tatiana ULiege; Mouithys-Mickalad, Ange ULiege et al

Poster (2019, November 21)

Since mitochondria play diverse crucial roles within the cell, mitochondrial dysfunction can have disastrous consequences. High resolution respirometry (HRR) is a new tool that allows the quantification ... [more ▼]

Since mitochondria play diverse crucial roles within the cell, mitochondrial dysfunction can have disastrous consequences. High resolution respirometry (HRR) is a new tool that allows the quantification of mitochondrial oxidative phosphorylation and electron transfer capacities. This tool allows an analysis of mitochondrial bioenergetics and the main substrate pathway of the cells that are of interest. In human, platelets and peripheral blood mononuclear cells have been used as biomarkers in diverse pathological models, as diabetes, atherosclerosis, cancer and neurodegenerative diseases. In the horse, HRR studies have been conducted on skeletal muscles samples. Using this technique, a so-called “mitochondrial syndrome” has been reported in equine atypical myopathy, an environmental intoxication, which is characterized by a severe decrease of mitochondrial respiratory capacity in affected individuals. However, the muscle biopsy procedure is difficult to use in equine clinical routine. Therefore, the aim of this study was to test whether mitochondrial function could be assessed on equine blood mononuclear cells with respirometry. Whole blood was collected in 9 ml EDTA tubes in six horses. Cells of interest (PBMC) were isolated with a density-separation medium (Lymphoprep™) following a standardized procedure. Electron paramagnetic resonance as well as HRR analysis were performed within the following 6 hours. With this isolation technique more than 1x106 PBMC/ml of whole blood were obtained. Respirometric analysis with PBMC taken from the same horses indicated a lack of reproducibility. This finding seems to be primarily due to a too long timeframe (i.e. ±Xh) between cell isolation and respirometric analysis. Additional studies indicated that when respirometry was performed within the first 2 hours after cell isolation, standard variation between samples was significantly reduced. Up to now a correlation between skeletal muscle fiber and PBMC mitochondrial bioenergetics in the equine species is not possible to be established. A standardization of the procedure is vital for reproducibility between laboratories and comparison between healthy and ill horses. The next steps are to compare the oxygraphic measures between equine PBMC and muscle biopsies, as well as the comparison of mitochondrial function between healthy and horses in extreme metabolic conditions (e.g atypical myopathy). [less ▲]

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See detailLa myopathie atypique : le top 10 des dernières nouvelles
Votion, Dominique ULiege; François, Anne-Christine ULiege; Renaud, Benoît ULiege et al

in Mespoulhès, Céline (Ed.) Proceeding Journée AVEF (Association des Vétérinaires Equins Français) (2019, November 15)

Cet écrit reprend le top 10 des dernières nouvelles relatives à la myopathie atypique dont certaines ne sont pas encore publiées.

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See detailÉvaluation des profils quantitatifs en MCPA-carnitine et acylcarnitines chez des chevaux atteints de myopathie atypique
Wouters, Clovis ULiege; Toquet, Marie-Pierre; Marcillaud-Pitel, Christel et al

in Mespoulhès-Rivière, Céline (Ed.) Proceeding Journée AVEF (Association des Vétérinaires Equins Français) (2019, November 15)

L’objectif de cette étude était de déterminer s’il existe un lien entre la mort de l’équidé et le diagnostic histologique avec les valeurs quantitatives de MCPA-carnitine et d’acylcarnitines, chez des ... [more ▼]

L’objectif de cette étude était de déterminer s’il existe un lien entre la mort de l’équidé et le diagnostic histologique avec les valeurs quantitatives de MCPA-carnitine et d’acylcarnitines, chez des chevaux MCPA-carnitine positif et présentant des signes cliniques évocateurs de myopathie atypique. [less ▲]

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See detailUntargeted metabolomics studies focusing on prognosis and resistance factors leading to the identification of new potential biomarkers in equine atypical myopathy
Wouters, Clovis ULiege; Toquet, Marie-Pierre; Renaud, Benoît ULiege et al

in Bayrou, Calixte; Delguste, Catherine; Douny, Caroline (Eds.) et al Proceedings of the 6th FARAH-Day (2019, November 08)

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See detailDiagnostic and prognostic value of hypoglycin A, methylenecyclopropyl acetic acidcarnitine and, acylcarnitines profile in horses with atypical myopathy
Renaud, Benoît ULiege; François, Anne-Christine ULiege; BOEMER, François ULiege et al

in Bayrou, Calixte; Delguste, Catherine; Douny, Caroline (Eds.) et al Proceedings of the 6th FARAH-Day (2019, November 08)

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See detailHypoglycin-A Intoxikation durch Bergahorn (Acer pseudoplatanus) bei Zootieren - eine wachsende Gefahr
Bunert, Carolin; Grund, Lisa; Ziemssen, Eva et al

Poster (2019, November)

Atypische Weidemyopathie ist eine bei Equiden bekannte Erkrankung, die durch die Aufnahme von toxinhaltigen Anteilen des Bergahorns (Acer pseudoplatanus) ausgelöst wird. Das Toxin Hypoglycin A ist in den ... [more ▼]

Atypische Weidemyopathie ist eine bei Equiden bekannte Erkrankung, die durch die Aufnahme von toxinhaltigen Anteilen des Bergahorns (Acer pseudoplatanus) ausgelöst wird. Das Toxin Hypoglycin A ist in den Samen und Sämlingen von Bergahorn-Pflanzen in besonders hoher Konzentration enthalten; entsprechend tritt die Erkrankung vor allem im Herbst und im Frühling auf, wenn die jeweiligen Pflanzenteile für Tiere erreichbar sind. Hypoglycin A wird zu MCPA-CoA metabolisiert, welches die Fettsäureoxidation in den Mitochondrien hemmt. In der Folge kommt es zu Energiemangel in lebenswichtigen Organen (Herz, Hirn, Atemmuskulatur) und einer massiven Rhabdomyolyse. Erkrankte Pferde zeigen Steifheit, Schwäche und Myoglobinurie, die Mortalitätsrate liegt bei 70-90%. 1 [less ▲]

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See detailPeripheral blood mononuclear cells to detect mitochondrial dysfunction in horses: a preliminary study
Kruse, Caroline ULiege; Art, Tatiana ULiege; Mouithys-Mickalad, Ange ULiege et al

Conference (2019, October 14)

Mitochondrial dysfunction has emerged as a ubiquitous cause of disease in human and animals. In the equine species, muscle fibers were analyzed to detect and assess defective mitochondrial function. Using ... [more ▼]

Mitochondrial dysfunction has emerged as a ubiquitous cause of disease in human and animals. In the equine species, muscle fibers were analyzed to detect and assess defective mitochondrial function. Using respirometry, a so-called “mitochondrial syndrome” has been reported in equine atypical myopathy, which is characterized by a severe decrease of mitochondrial respiratory capacity in affected individuals [1] . Up to now, respirometric studies in horses have been successfully conducted on skeletal muscles samples. However, the muscle biopsy procedure is rather invasive and therefore difficult to use in equine clinical routine. Despite the fact that peripheral blood mononuclear cells (PBMC) have been used in human medicine to assess mitochondrial function, there is only one study comparing PBMC and skeletal muscle oxidative capacity in monkeys so far [2]. Therefore, the aim of this study was to test whether mitochondrial function can be assessed on equine blood cells with respirometry, according to the fact that blood is a biological sample easier to collect than muscle. Whole blood was collected in 9 ml EDTA tubes in four horses. Cells of interest (PBMC) were isolated with a density-separation medium (Lymphoprep™) within one hour of sampling, following a standardized procedure. Electron paramagnetic resonance as well as the respirometric analysis were performed within the following 4 hours. The preliminary tests show that recorded respirations are similar to those documented in humans. However, a lack of reproducibility of respirometric analysis with PBMC taken from the same horses was observed. Therefore, a correlation between skeletal muscle fiber and PBMC mitochondrial bioenergetics in the equine species is, up to now, not possible to be established. Nevertheless, an efficient PBMC isolation protocol was established and mitochondrial respiration with respirometry was obtained. The cause of the variations within the same individual is still under examination. [less ▲]

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See detailRole of Sirtuin 3 in the differentiation of 3T3-L1 preadipocytes
Arnould, Thierry; Storder, Julie; Kruse, Caroline ULiege et al

Conference (2019, October)

An adaptive capacity of white adipose tissues is essential to match energy intake and expenditure. Part of this adaptation is driven by the enrollment of new adipocytes, a phenomenon referred to ... [more ▼]

An adaptive capacity of white adipose tissues is essential to match energy intake and expenditure. Part of this adaptation is driven by the enrollment of new adipocytes, a phenomenon referred to “adipogenesis”. Our current efforts are dedicated to the investigation of the putative role of the Sirtuin 3 (SIRT3), a NAD+-dependent deacetylase and master regulator of several mitochondrial functions, in the differentiation of preadipocytes in white adipocytes. This is of particular relevance as mitochondrial biogenesis and activation of oxidative metabolism are promoted by SIRT3 and are known to be required for proper adipogenic differentiation. Moreover, SIRT3 was already reported to be required for proper differentiation of various other cell types such as skeletal myocytes [1] and brown adipocytes [2]. White 3T3-L1 adipoblasts were differentiated in adipocytes in the presence or in the absence of SIRT3 (invalidation using CIRSPR/Cas9-DN technology) and the expression of SIRT3 as well as markers of its activity were analysed during a differentiation programme of 12 days. In addition, ROS production as well as respiration (Oxygraph-2k, ORBOROS Instruments Corp) in cells in the presence or in the absence of SIRT3 were studied. The results show that the expression of SIRT3 is induced during the differentiation programme. In addition, the absence of SIRT3 is reflected by a delay (but not the inhibition) of preadipocyte differentiation as demonstrated by a reduced content of triacylglycerols. We are currently investigating the underlying mechanisms that could postpone adipogenesis in the absence of the mitochondrial deacetylase such as modifications in reactive oxygen species production and putative alterations of OXPHOS that could connect SIRT3 to proper development of the adipose phenotype. In conclusion, we showed that SIRT3 activity accompanies the adipogenic programme of 3T3-L1 preadipocytes and that the enzyme plays an important role in the kinetics of the differentiation programme. The identification of SIRT-3 dependent mechanisms playing a role in the differentiation of preadipocytes is ongoing. [less ▲]

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See detailMyopathie atypique : les différentes sources d’intoxication. Comment gérer le risque ?
Renaud, Benoît ULiege; François, Anne-Christine ULiege; Marcillaud-Pitel, Christel et al

in Proceedings Journées Sciences et Innovations équines (2019, May)

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See detailL’érable sycomore responsable de la myopathie atypique des équidés, une maladie émergente en Europe
Votion, Dominique ULiege

Scientific conference (2019, March 08)

Recherche en cours :La myopathie atypique des équidés résulte de l’ingestion d’hypoglycine A (HG A) contenue dans les samares et plantules de l’érable sycomore. Au vu de la fulgurance de la maladie (temps ... [more ▼]

Recherche en cours :La myopathie atypique des équidés résulte de l’ingestion d’hypoglycine A (HG A) contenue dans les samares et plantules de l’érable sycomore. Au vu de la fulgurance de la maladie (temps de survie moyen: 24h), même si un antidote était trouvé, celui-ci arriverait souvent trop tard. Aussi, la prévention est, et restera, l’outil clé de la lutte contre cette maladie. Les objectifs la recherche menée sont de proposer des outils de prévention (à l’échelle de la prairie et de l’animal) validés scientifiquement contre cette maladie d’origine environnementale et de mettre à disposition du secteur équin un système d’alerte basé sur l’identification des facteurs de toxicité environnementale. En parallèle, des recherches sont menées pour mieux diagnostiquer et traiter la myopathie atypique. [less ▲]

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See detailPotential new sources of hypoglycin A poisoning for equids kept at pasture in spring: a field pilot study
Votion, Dominique ULiege; Habyarimana, J.A.; Scippo, Marie-Louise ULiege et al

in Veterinary Record: Journal of the British Veterinary Association (2019)

The first objective of this pilot study was to measure the concentration of HGA in samaras of sycamore trees fallen on the ground and then in seedlings from germination. The subsequent objective was to ... [more ▼]

The first objective of this pilot study was to measure the concentration of HGA in samaras of sycamore trees fallen on the ground and then in seedlings from germination. The subsequent objective was to examine other possible sources of HGA intoxication. [less ▲]

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See detailMyopathie atypique : les sources connues et suspectées d’intoxication
Renaud, Benoît ULiege; François, Anne-Christine ULiege; Rouxhet, Serge ULiege et al

in Pratique Vétérinaire Équine (2019), 201

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See detailPoint sur les outils diagnostiques et pronostiques de la myopathie atypique
Votion, Dominique ULiege; BOEMER, François ULiege; Marcillaud-Pitel, Christel et al

in Pratique Vétérinaire Équine (2019), 201

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