References of "VRANKEN, Laura"
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See detailSimultaneous measurement of 25(OH)-vitamin D and 24,25(OH)2-vitamin D to define cut-offs for CYP24A1 mutation and vitamin D deficiency in a population of 1200 young subjects.
Cavalier, Etienne ULiege; Huyghebaert, Loreen ULiege; ROUSSELLE, Olivier ULiege et al

in Clinical chemistry and laboratory medicine (2020), 58(2), 197-201

Background Simultaneous measurement of 25(OH)D and 24,25(OH)2D is a new tool for predicting vitamin D deficiency and allows evaluating CYP24A1 lack of function. Interpretation of 24,25(OH)2D should be ... [more ▼]

Background Simultaneous measurement of 25(OH)D and 24,25(OH)2D is a new tool for predicting vitamin D deficiency and allows evaluating CYP24A1 lack of function. Interpretation of 24,25(OH)2D should be performed according to 25(OH)D levels and a ratio, called the vitamin D metabolite ratio (VMR) has been proposed for such a purpose. Unfortunately, the VMR can be expressed in different ways and cannot be used if 24,25(OH)2D concentrations are undetectable. Here, we propose evaluating the enzyme activity taking into consideration the probability that a normal population presents undetectable 24,25(OH)2D concentrations according to 25(OH)D levels. We thus retrospectively measured 25(OH)D and 24,25(OH)2D in a population of 1200 young subjects to evaluate the 25(OH)D threshold above which the enzyme was induced. Methods Serum samples from 1200 infants, children, adolescent and young adults were used to simultaneously quantify 25(OH)D and 24,25(OH)2D by LCMS/MS. Results Median (interquartile range [IQR]) levels were 20.6 (14.4-27.2) ng/mL for 25(OH)D. 172 subjects (14.3%) presented 24,25(OH)2D values below the LOQ. When 25(OH)D values were <11 ng/mL, 63.1% of subjects presented undetectable 24,25(OH)2D concentrations. Percentage decreased with increasing 25(OH)D values to become 19.7% for 25(OH)D comprised between 12 and 15 ng/mL, 5.1% for 25(OH)D between 16 and 20 and 0.7% for 25(OH)D >21 ng/mL. Conclusions We suggest using a statistical approach to evaluate CYP24A1 function according to 25(OH)D concentrations. Our results also show that vitamin D deficiency, as defined biochemically, could be around 20 ng/mL in infants, children, adolescent and young adults and that vitamin D deficiency could be evaluated on a more individual basis. [less ▲]

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See detailImpact of Metformin on vitamin B12 status
VRANKEN, Laura ULiege; FONTAINE, Corentin ULiege; VALDES SOCIN, Hernan Gonzalo ULiege et al

Poster (2019, November 15)

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See detailBiomarkers of neonatal stress assessment: A prospective study.
ROUATBI, Hatem ULiege; Zigabe, Serge; Gkiougki, Eva et al

in Early Human Development (2019), 137

INTRODUCTION: Early diagnosis of perinatal asphyxia, the major cause of neonatal mortality and morbidity, might be improved by the detection of neonatal stress biomarkers such as cardiac troponin (CTn)T ... [more ▼]

INTRODUCTION: Early diagnosis of perinatal asphyxia, the major cause of neonatal mortality and morbidity, might be improved by the detection of neonatal stress biomarkers such as cardiac troponin (CTn)T, CTnI, NT-Terminal-pro-Brain Natriuretic Peptide (NT-pro-BNP), copeptin, and high sensitivity C-reactive protein (hs-CRP). However, reference values in neonates are lacking. The objective of our study was therefore to establish a reference range of these biomarkers in healthy full term newborns and to analyze the influence of delivery mode on their cord blood concentrations. PATIENTS AND METHODS: CTnT, CTnI, NT-pro-BNP, Copeptin and hs-CRP levels were determined in 201 neonates enrolled in this prospective study and correlated to the delivery mode and post-natal outcome. RESULTS: Using the 99th percentile, the upper reference limit in healthy newborns was established for all biomarkers. Neonates born after complicated delivery had significantly higher values of CTnT, CTnI and Copeptin than those born after uncomplicated delivery. In the multiple regression models with CTnT as dependent variable, the delivery mode was the statistically significant independent variable. CONCLUSION: In this study, we established reference values of cord blood concentrations of cardiac stress biomarkers in healthy newborns. We showed that cardiac-related birth stress is dependent on delivery mode. [less ▲]

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See detailEvaluation of Metformin impact on vitamin B12 status
VRANKEN, Laura ULiege; FONTAINE, Corentin ULiege; LUKAS, Pierre ULiege et al

Poster (2019, May)

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See detailCardiac stress biomarkers in neonates: role of the delivery mode
ROUATBI, Hatem ULiege; Gkiouki, E.; Zigabe, Serge et al

Poster (2019, May)

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See detailComparison of cardiac biomarker dynamics in marathon, semimarathon and untrained runners: what is the impact on results interpretation?
Le Goff, Caroline ULiege; Lennartz, Lieselotte; VRANKEN, Laura ULiege et al

in Journal of Laboratory and Precision Medicine (2019), 4(6), 10

Background: Cardiac biomarkers elevations have been described after running exercise. Objective of our study was to check cardiac biomarker dynamics in well trained athletes and untrained middle aged ... [more ▼]

Background: Cardiac biomarkers elevations have been described after running exercise. Objective of our study was to check cardiac biomarker dynamics in well trained athletes and untrained middle aged apparently healthy men and to highlight the impact on the lab results interpretation in emergency department. Methods: Cardiac biomarkers for ischemic condition, cardiac stretch and fibrotic processes were tested in different type of runners before, directly after and 3 hours after running. Markers for inflammation, muscle disease and renal function were also measured. Results: Cardiac biomarker levels between groups were not statistically different in the pre-exercise samples for natriuretic peptides [B-type natriuretic peptide (BNP), N-terminal Pro BNP (NT-ProBNP)] and galectin-3 (Gal-3), only Troponin I levels were higher. Directly after exercise, all cardiac biomarker levels were higher compared to the baseline, Gal-3 and BNP levels decreased 3 hours after completion of the run.Troponin values continued to increase with highest levels 3 hours after exercise. Troponin T, NT-Pro BNP and Gal-3 also showed significant correlation to markers of inflammation, fibrosis and renal function. Conclusions: Exercises of different intensity can be associated with biochemical abnormalities and longterm consequences are unknown. In chest pain patients presenting to the emergency department possible impact of exercise on test results, especially Troponin, should be checked. [less ▲]

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See detailImpact of the creatinine-based equations used on the chronic kidney disease classification in a large laboratory database
masson, grégoire; VRANKEN, Laura ULiege; KRZESINSKI, Jean-Marie ULiege et al

in Nephrology Dialysis Transplantation (2019)

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See detailValidation of 24 blood gas analyzers, GEM 5000 Premier
GADISSEUR, Romy ULiege; MUSSO, Giuseppe ULiege; Cavalier, Etienne ULiege et al

Poster (2018, September 28)

Introduction: Point-of-care blood gas test results may help to take therapeutic decision by their immediate impact on patient care. Recently, a novel cartridge-type blood gas analyzer, the GEM Premier ... [more ▼]

Introduction: Point-of-care blood gas test results may help to take therapeutic decision by their immediate impact on patient care. Recently, a novel cartridge-type blood gas analyzer, the GEM Premier 5000 (IL-Werfen) was commercialized and 24 analyzers were installed at the University Hospital of Liège. One of them was installed in the main central laboratory, the 23 other ones were installed into 19 patient care units. Before the implementation, we evaluated the analytical performance of all the 24 GEM Premier 5000, for the determination of whole blood pH, pCO2 and pO2, electrolytes (Na+, K+, Cl−), ionized calcium (iCa2+), glucose, lactate and co-oximetry parameters ((total hemoglobin (tHb), oxyhemoglobin (O2Hb), carboxyhemoglobin (COHb), methemoglobin (metHb), deoxyhemoglobin (HHb)). Method: First, we evaluated the performance of the GEM Premier 5000 dedicated to the central lab, so-called “referent analyzer”, with 3 levels of External Quality Controls material (EQC RNA Medicals). CLSI EP5 recommends 2 replicates per run, 1 or 2 runs per day, for a minimum of 20 days. Nevertheless, we analyzed the 3 levels of EQC, 2 replicates per run, 2 runs per day during 5 consecutive days. Afterwards, on the whole 24 GEM Premier 5000, we analyzed aqueous QC material (Werfen GEM System Evaluator, level 1-2), 3 replicates within a single run, once per day, during 5 consecutive days. We determined the manufacturer's claim for Within-Run and Total precisions for each. Co-oxymetry parameters were not evaluated on 4 analyzers. Then, we compared the all 23 analyzers to the “referent analyzer” of the central lab. Therefore, for each parameter, we showed in a Youden diagram all the results obtained by 23 analyzers. The position of the acceptance ranges were shown graphically using the specifications for Acceptable(%) Root Mean Standard Deviation (RMSD) proposed by the German Guidelines for Quality (RILIBAK) for whole blood parameter, for different ranges of parameters. Results: The results showed a good correlation between analyzers excepted for some parameters. Lactate and MetHb: level 2 were often over-estimated when compared to “Reference Analyzer”. It could be explained by the fact that this IQC level contains very low Lactate and MetHb rates. The pO2 level2: some results were over estimated (random errors >< cassette reagents >< low values). The pO2 level1: over-estimated with 8 analyzers letting us think that the cassette reagent of our “Reference Analyzer” had a bias in the lower range. Conclusion: Performance evaluation of a large cluster of Blood Gas Analyzers is always a challenge for a Hospital. Accreditation is one of the main goal in each Belgian laboratory. Hospital Accreditation is also discussed in Belgium. This study shows an interesting approach to validate Blood Gas Analyzers for highlighting data. Based on our study results, we estimated that the evaluated instrument are a suitable blood gas analyzer for both POCT and laboratory use. [less ▲]

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See detailCARDIAC BIOMARKERS FLUCTUATION IN RUNNERS OF MARATHONS, SEMI-MARATHONS AND UNTRAINED RUNNERS
Le Goff, Caroline ULiege; VRANKEN, Laura ULiege; Kaux, Jean-François ULiege et al

in 23rd Annual Congress of the ECSS (2018, July)

INTRODUCTION: Regular exercise like running is one important part of the prevention program of cardiovascular disease. There are several studies on biomarker changes during marathons especially cardiac ... [more ▼]

INTRODUCTION: Regular exercise like running is one important part of the prevention program of cardiovascular disease. There are several studies on biomarker changes during marathons especially cardiac biomarkers have been studied and mild to moderate elevations have been described as a results of a running exercise Exact underlying mechanism for these biomarker elevations reflecting physiological or even pathobiological changes is unknown and less trained athletes might exhibit a higher risk compared to well trained. The aim of our study was to compare three cardiac biomarkers for ischemic condition , cardiac stretch and fibrotic processes were tested in different type of runners, trained marathon and semi-marathon runners and untrained runners before, directly after and 3 hours after the running exercise. METHODS: 23 marathon runners, 15 semi-marathon runners <44.1±8.4yo> and 17 healthy sedentary subjects < 37± 4.4 yo> were enrolled in our study. Blood samples were taken just before , just after and 3 hours after the race, centrifuged, aliquoted and stored frozen at -80°C before further analysis. The study was approved by the Ethical Committee of our University Hospital. The analyses were performed on the Abbott ARCHITECT i2000SR for the hs cTnI, BNP and Gal-3 and on the C8000 for hs-cTnT and NTproBNP according to the manufacturer’s instructions for use. RESULTS: In all 3 running groups there is an increase of cardiac biomarkers Troponin I, BNP, Galectin-3 and NT-ProBNP after completion of the physical exercise. Biomarkers increase is depending on the intensity and duration of the exercise and is higher in long distance marathon and semi-marathon runners compared to the control group with a 1 hour run. Cardiac biomarker levels between trained marathon and semi-marathon runners were not statistically different in the pre-exercise baseline samples for BNP, NT-Pro-BNP and Galectin-3. Compared to untrained runners only Troponin I levels were higher in baseline sample of marathon runners when compared to controls, cardiac Troponin T was less significant. CONCLUSION: In conclusion, the question whether running exercise of different intensity could be harmful to the heart has no simple answer. We could show that running exercise can be associated with biochemical abnormalities that may reflect adverse consequences on the heart like possible micro necrosis, oxidative stress, fibrosis and myocardial stretch. With exception of Troponin where levels continue to raise after end of running, NPs and Gal-3 levels normalized relatively fast after the exercise. The possible harmful effect of longer term cardiac consequences of repeated intensive sport acitvities still needs to be demonstrated. [less ▲]

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