References of "Tutunaru, Alexandru-Cosmin"
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See detailEffect of antimicrobial drug on lung microbiota in healthy dogs.
Fastrès, Aline ULiege; Vangrinsven, Emilie ULiege; Tutunaru, Alexandru-Cosmin ULiege et al

in Journal of Veterinary Internal Medicine (2018, October 12)

In human and dogs, baseline differences in lung microbiota (LM) have been associated with important clinical features in chronic lung diseases (CLD) and there is growing evidence that an altered LM ... [more ▼]

In human and dogs, baseline differences in lung microbiota (LM) have been associated with important clinical features in chronic lung diseases (CLD) and there is growing evidence that an altered LM contributes to disease pathogenesis. The common use of antibiotic drugs throughout the management of CLD likely represents a major confounding factor in the study of the LM. However, the effect of antibiotic treatment on the LM in healthy individuals has not been specifically investigated yet. The aim of the present study was to assess the short and medium-term effect of an oral treatment with a large spectrum antimicrobial drug on the LM in healthy dogs. Six healthy experimental beagle dogs were included. Amoxycillin/clavulanic acid (AC) was administered at a dose of 20 mg/kg twice daily for 10 days. In each dog, bronchoalveolar lavage fluid (BALF) was collected at 3 different timepoints: before administration of AC (J0) and immediately (J10) as well as 16 days (J26) after interruption of AC. In each BALF, total and differentiated cell counts were obtained and metagenetic analyses were performed on the V1-V3 hypervariable region of 16S rDNA after total bacterial DNA extraction and sequencing on a MiSeq Illumina sequencer. Taxonomical assignation and microbiota community analysis were done with MOTHUR V1.35 with an OTU clustering distance of 0.03. Statistical comparisons between events for microbiota community and BALF cell counts were made using Friedman test and post-hoc t-tests with Bonferroni correction. Statistical differences in bacterial population relative abundance between timepoints were assessed using a mixed linear model with FDR correction for multiple comparisons. Administration of AC did not induce significant changes in BALF cellular counts and had no effect on the richness, evenness and alpha diversity. Bacteroidetes and Proteobacteria abundance increased from J0 to J10 (mean: 12.0 to 24.1, and 12.3 to 26.2%, respectively; p<0.001) and decreased at J26 (13.1 and 9.3%; p<0.001). Firmicutes abundance decreased from J0 to J10 (45.6 to 18.3%; p<0.001) and increased at J26 (28.8%; p<0.001). Actinobacteria abundance increased at J26, compared with J0 and J10 (43.7 compared with 23.2 and 23.5% respectively; p<0.001). We failed to show significant differences between timepoints at the level of genera and species. In conclusion, in healthy dog, oral administration of a commonly used large spectrum antimicrobial drug induces significant changes in microbial population abundance at the phyla level. Most but not all of these changes normalize within 2 weeks after discontinuation of the drug. [less ▲]

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See detailInvestigation of serum Krebs Von den Lungen 6 concentration as a predisposing factor and in the diagnosis of canine idiopathic pulmonary fibrosis in the West Highland white terrier.
Fastrès, Aline ULiege; Roels, Elodie ULiege; Bolen, Géraldine ULiege et al

Poster (2018, September 06)

Canine idiopathic pulmonary fibrosis (CIPF) mimics human idiopathic pulmonary fibrosis (IPF) and affects old dogs from the West Highland white terrier (WHWT) breed. Due to difficulties to both diagnose ... [more ▼]

Canine idiopathic pulmonary fibrosis (CIPF) mimics human idiopathic pulmonary fibrosis (IPF) and affects old dogs from the West Highland white terrier (WHWT) breed. Due to difficulties to both diagnose early and follow the course of pulmonary fibrosis, numerous biomarkers have been studied in both species. In humans, the mucin Krebs Von den Lungen 6 (KL-6) has consistently been found to be increased in the serum from patients with interstitial lung disease, including IPF and is possibly involved in the IPF pathophysiological process. In CIPF, it has not yet been assessed. In this study, we assessed the diagnostic value of serum KL-6 concentration in CIPF as well as the association between serum KL-6 concentrations and breed predisposition for the disease. Blood was obtained from 5 groups of dogs: WHWTs affected with CIPF (n=20, 14 females (F)/6 males (M), mean age=11.0y, range [5.2-14.5]), healthy aged-matched WHWTs (n=18, 11F/7M, 10.5y [5.3-15.3]), and healthy dogs from other breeds not predisposed for CIPF: Jack Russel terriers (JR) (n=13, 8F/5M, 6.0y [3.0-9.1]), Malinois Belgian shepherds (MBS) (n=13, 5F/8M, 6.5y [2.8-10.8]) and King Charles spaniels (KCS) (n=13, 9F/4M, 6.5y [3.6-9.3]). Serum was stored at -80°C until batched analysis. KL-6 concentration was measured with a commercially available ELISA test (Canine Krebs Von den Lungen 6 Elisa kit, amsbio). Results were compared using a covariance analysis including the 5 groups defined above, gender and an interaction between groups and gender together with the effect of the age. The analysis was followed by post-hoc comparisons between groups using t-tests. Results show that only the effect of groups was significant (p=0.010). Serum KL-6 concentration was higher in healthy WHWTs (4.8+/-4.0 ng/ml) compared with KCS (1.6+/-1.8 ng/ml) and MBS (2.2+/-1.0 ng/ml) (p=0.001 and 0.013 respectively) and higher in JR (4.1+/-2.9 ng/ml) compared with KCS (p=0.016). There was no difference between CIPF (3.2+/-1.9 ng/ml) and healthy WHWTs (p=0.072). In conclusion, differences in serum KL-6 concentrations were found amongst breeds in healthy dogs, with higher concentrations observed in terrier breeds. Whether this may reflect a predisposing factor for CIPF development merits further investigations. Unlike what has been found in human IPF, KL-6 is not a good serum biomarker for diagnosis as there was no difference between CIPF and unaffected WHWTs. [less ▲]

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See detailAnesthesia Case of the Month
Tutunaru, Alexandru-Cosmin ULiege; Sandersen, Charlotte ULiege

in Journal of the American Veterinary Medical Association (2017), 250(9), 984-987

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See detailUse of cis-atracurium to maintain moderate neuromuscular blockade in experimental pigs
Tutunaru, Alexandru-Cosmin ULiege; Dupont, Julien ULiege; Serteyn, Didier ULiege et al

in Veterinary Anaesthesia and Analgesia (2017)

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See detailComparison of clinical effects of epidural levobupivacaine morphine versus bupivacaine morphine in dogs undergoing elective pelvic limb surgery
Cerasoli, Ilaria; Tutunaru, Alexandru-Cosmin ULiege; Cenani, Alessia et al

in Veterinary Anaesthesia and Analgesia (2017)

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See detailComparison of clinical effects of epidural levobupivacaine morphine versus bupivacaine morphine in dogs undergoing elective pelvic limb surgery
Cesaroli, Ilaria; Tutunaru, Alexandru-Cosmin ULiege; Cenami, Alessia et al

in Veterinary Anaesthesia and Analgesia (2017), 44

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See detailNeuromuscular blockade as part of an anaesthetic protocol for cataract surgery in an alpaca
Tutunaru, Alexandru-Cosmin ULiege; Storms, Goedele ULiege; Monclin, Sébastien ULiege et al

in Journal of Camelid Science (2016), 9

A six-month-old alpaca was presented to the veterinary teaching hospital of the University of Liège (Belgium) with a bilateral cataract. A decision was made to perform bilateral cataract surgery after an ... [more ▼]

A six-month-old alpaca was presented to the veterinary teaching hospital of the University of Liège (Belgium) with a bilateral cataract. A decision was made to perform bilateral cataract surgery after an ocular ultrasound and electroretinogram were performed to assess the posterior segment and retinal function, respectively. As a premedication, the alpaca received xylazine, butorphanol and midazolam intramuscularly. Anaesthesia was induced subsequently with ketamine and midazolam intravenously and maintained with isoflurane on oxygen/air mixture. The alpaca was mechanically ventilated during surgery. Neuromuscular blockade was induced and maintained with cis-atracurium intravenously. The dose of cis-atracurium was permanently adapted using a peripheral nerve stimulator. This case report brings new knowledge regarding the use and dosage of cis-atracurium in alpacas. [less ▲]

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See detailEvaluation of the effect of oral omeprazole on canine cerebrospinal fluid production: A pilot study.
Girod, Maud ULiege; Allerton, F.; Gommeren, Kris ULiege et al

in Veterinary Journal (2016), 209

Administration of omeprazole by ventriculo-cisternal perfusion or intravenously has been shown to decrease cerebrospinal fluid (CSF) production in dogs and rabbits. Oral omeprazole has consequently been ... [more ▼]

Administration of omeprazole by ventriculo-cisternal perfusion or intravenously has been shown to decrease cerebrospinal fluid (CSF) production in dogs and rabbits. Oral omeprazole has consequently been recommended to reduce CSF production in dogs with conditions in which clinical signs may be attributable to an accumulation of CSF in the central nervous system (e.g. hydrocephalus, syringomyelia). The albumin quotient (QAlb), the ratio between CSF and serum albumin concentration, has been proposed as a reliable means to evaluate CSF production; decreasing CSF production should cause an increase in QAlb. The aims of this study were to assess the effect of oral administration of omeprazole on QAlb in dogs and to compare two methods to assess CSF albumin concentration. Fifteen healthy Beagle dogs received omeprazole (1.2 mg/kg/day) orally for 14 days; CSF and blood were obtained before and after treatment. CSF albumin concentrations were evaluated by nephelometry and high-resolution protein electrophoresis. Regardless of the method used for measuring albumin, QAlb did not change significantly following oral omeprazole administration, suggesting that CSF production in healthy dogs may not be affected by chronic oral therapy with omeprazole. [less ▲]

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See detailHeart rate variability as a measure of comfort in the anaesthetised horse
Lacroix, A.; Gougnard, A.; Cerri, S. et al

in Proceedings of the Farah Day (2015)

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See detailHeart rate variability as a measure of comfort in the anaesthetised horse
Lacroix, Alice; Gougnard, Alexandra ULiege; Cerri, Simona ULiege et al

Poster (2015)

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See detailEffects of omeprazole on the canine cerebrospinal fluid composition
Girod, Maud ULiege; Allerton, Fergus; Gommeren, Kris ULiege et al

in Proceedings of the 23rd ECVIM-CA CONGRESS (2013, September)

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See detailClinical Assessment of alfaxolone in rabbits
Tutunaru, Alexandru-Cosmin ULiege; Sonea, Alexandru; Drion, Pierre ULiege et al

Poster (2012, October 19)

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See detailClinical aspects of alfaxalone use in rabbits. Partial results.
Tutunaru, Alexandru-Cosmin ULiege; Sonea, P; Drion, Pierre ULiege et al

in BCLAS, ESLAV (Ed.) Proceedings BCLAS, ESLAV & ECLAM Scientific Meeting (2012, October 15)

Alfaxalone, a synthetic neuroactive steroid, has been shown to induce smooth general anesthesia in wild and companion rabbits. The aim of the study was to test the effect of alfaxolone in experimental ... [more ▼]

Alfaxalone, a synthetic neuroactive steroid, has been shown to induce smooth general anesthesia in wild and companion rabbits. The aim of the study was to test the effect of alfaxolone in experimental rabbits without concomitant oxygen supplementation.Respiratory status was assessed by measuring the duration of the post-induction apnea, the respiratory rate, the oxygen saturation of hemoglobin in the peripheral blood (SpO2) and the end-tidal CO2 (EtCO2) Cardiac function was assessed by monitoring heart rate using a pulse oximeter. The degree of anesthesia was monitored by evaluating ear and paw pinch reflex, as well as ocular signs such as nystagmus, exophtalmia and the loss of palpebral and corneal reflexes. [less ▲]

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See detailClinical efficacy and safety of levobupivacaine and morphine by lumbosacral epidural route for elective hindlimb surgery in dogs
Cerasoli, Ilaria; Cenani, Alessia; Tutunaru, Alexandru-Cosmin ULiege et al

Scientific conference (2012)

Detailed reference viewed: 17 (0 ULiège)