References of "Tonus, Céline"
     in
Bookmark and Share    
Full Text
See detailMOOCs et évaluation : état de l’art et analyse de cas
Tonus, Céline ULiege; De Lemos Esteves, Frédéric ULiege; Fettweis, Véronique ULiege et al

Conference (2019, April 27)

Depuis 2012, les MOOCs (Massive Open Online Courses – Cours en ligne ouverts et massifs) ont pris une place qu’il est impossible d’ignorer dans le paysage de l’enseignement. A ce jour, environ 11.000 ... [more ▼]

Depuis 2012, les MOOCs (Massive Open Online Courses – Cours en ligne ouverts et massifs) ont pris une place qu’il est impossible d’ignorer dans le paysage de l’enseignement. A ce jour, environ 11.000 MOOCs sont accessibles, portés par quelques 900 universités et rassemblant plus de 100 millions d’apprenants issus de tous les coins de la planète. L’évaluation de apprentissages au sein de ces dispositifs est une problématique importante. Comment évaluer de manière valide plusieurs milliers d’apprenants actifs dans un MOOC ? Comment, dans ces conditions, offrir un feedback de qualité ? Faut-il certifier ces formations et comment garantir alors la valeur de cette certification ? Telles sont les questions qui seront abordées dans la première partie de la conférence. La seconde partie analysera, à l’aide du modèle « Prisme» (Leclercq, 2006 ; Gherib, Dujardin & Verpoorten, 2016) les différentes pratiques d’évaluation développées au sein des MOOCs produits par l’Université de Liège. [less ▲]

Detailed reference viewed: 15 (3 ULiège)
Full Text
Peer Reviewed
See detailA tutored online remedial course in veterinary Histology: For which students? For which perceptions and results?
Tonus, Céline ULiege; Jérôme, Françoise ULiege; Antoine, Nadine ULiege

Poster (2018, May)

Introduction and aim of the study. An online remedial course was associated to Histology practical teaching in the third year of the Bachelor's degree in the Faculty of Veterinary Medicine of the ... [more ▼]

Introduction and aim of the study. An online remedial course was associated to Histology practical teaching in the third year of the Bachelor's degree in the Faculty of Veterinary Medicine of the University of Liège. The main characteristics of this program were (i) voluntary enrolment, (ii) structured and progressive learning, (iii) individualized guidance and (iv) the opportunity for each student to work at his/her own pace. For each organ system, three levels of exercises were developed by combining the institutional e-learning environment (eCampus) and the virtual microscopy application Cytomine/Shareview. Level 1 consisted in short exercises aiming to revise the theoretical notions essential to the proper understanding of histological observations. Level 2 consisted in complete and guided description of histological slides very similar to those observed during in-class practical labs. Level 3 consisted in identification and description of unknown histological slides. The present study aimed at determining the learning profiles of participants in the remedial course and at making out the course’s effects on each profile in terms of engagement, perceptions and performance. Material and methods. Engagement and performance of each student were recorded during in-class practical labs and during remedial course. At the end of the remedial course and before summative assessment, students' perception was evaluated by an on-line survey. Performance to the summative assessment was also recorded. Results. Despite the small number of participants (n=22), four learning profiles could be determined. Profiles differ from each other essentially in terms of students’ strategic choices when entering the course and regarding their engagement in remedial exercises. Students' perceptions in relation to the remedial course were generally positive, regardless of the learning strategy they adopted. Effects on performance in summative assessment were only observable for students who developed engaged or strategical learning profiles. [less ▲]

Detailed reference viewed: 38 (19 ULiège)
Full Text
Peer Reviewed
See detailÉvaluer formativement en 1er Bac à l’université, et après ? Focale sur un outil de feedback et de feed forward longitudinal et personnalisé dans un cours d’Anglais à distance.
Tonus, Céline ULiege; Bouvy, Christine ULiege; Leduc, Laurent ULiege

in Actes du 30e colloque de l'ADMEE-Europe: L’évaluation en éducation et en formation face aux transformations des sociétés contemporaines (2018)

A l’heure où, dans les sociétés occidentales notamment, les thèmes de l’aide à la réussite ou de la "First-Year Experience" en milieu universitaire font l’objet d’une attention de plus en plus soutenue et ... [more ▼]

A l’heure où, dans les sociétés occidentales notamment, les thèmes de l’aide à la réussite ou de la "First-Year Experience" en milieu universitaire font l’objet d’une attention de plus en plus soutenue et appellent une prise en considération accrue de l’hétérogénéité forcément élevée des publics d’étudiants primo-arrivants, les projets pédagogiques axés sur les pratiques d’Assessment for Learning et de feedbacks formatifs figurent, dans la littérature sur le sujet, parmi les leviers à activer par priorité dans cette optique, notamment au regard de leur proximité évidente avec les approches visant à personnaliser la formation. Développé depuis 2013 à l’Université de Liège en suivant une logique d’ancrage facultaire, le projet "Feedback 1er Bac" propose aux enseignants en charge des étudiants de 1ère année une formule originale d’accompagnement à la conception et l’intégration dans les cours d’initiatives pédagogiques affiliées aux théories de l’AfL ou du feedback voire, pour certaines réalisations, à celles de la personnalisation. <br />Après avoir introduit brièvement les tenants et aboutissants du projet "Feedback 1er Bac", la présente communication propose tout d’abord d’établir, parmi l’ensemble des initiatives développées dans le cadre du programme (plus de 70 sur les premières facultés), un relevé non exhaustif de celles répondant au double critère de logique de personnalisation des feedbacks aux étudiants et de réalisme au niveau de la charge de l’enseignant. <br />Dans un second temps, nous envisagerons en détails le cas d’un dispositif de ce type, développé au sein d'un cours d'Anglais destiné à des étudiants de première année en Médecine vétérinaire. Celui-ci consiste en un tableau de bord dynamique, personnalisé en ligne pour les étudiants et articulé autour des feedbacks obtenus à une série de six tests formatifs successifs, feedbacks que l’outil vise à dépasser suivant une politique de feed forward systématique, propre à susciter l’autorégulation des utilisateurs. Après une analyse des lignes de forces du dispositif, abordées par le prisme de deux modèles théoriques de l’AfL (Nicol, 2009 ; Leduc et al., 2017), la communication se penchera sur l’évaluation de son impact ainsi que sur l’analyse des données objectives et subjectives collectées pour éclairer sa réception par les utilisateurs. [less ▲]

Detailed reference viewed: 104 (45 ULiège)
Full Text
See detailContribution à la mise en place d’une plateforme de transgénèse chez la volaille domestique: culture, cryopréservation et ingénierie génétique de cellules germinales primordiales.
Tonus, Céline ULiege

Doctoral thesis (2017)

Primordial germ cells (PGCs) are the intermediate of choice for domestic fowl transgenesis and genome editing. These can support large transgenes insertions and, as precursors of the germline, can ... [more ▼]

Primordial germ cells (PGCs) are the intermediate of choice for domestic fowl transgenesis and genome editing. These can support large transgenes insertions and, as precursors of the germline, can transmit a genetic modification to the next generation. Moreover, given the difficulties for cryopreserving avian gametes (spermatozoa and, above all, oocytes), PGCs represent the material of choice for germplasm preservation in these species. Despite the obvious advantages of PGCs use, recently described related technologies appear generally as more difficult to pursue, and as less effective than expected. In this context, we have developed and optimized some of them which appear as essential for carrying complex chicken genome engineering projects. We first focused on improving the effectiveness of culture and cryopreservation of PGCs. By developing a culture method in inserts, ending up with an efficiency close to 40 %, we were able to isolate and long-term cultivate chickens PGCs from 3 commercial breeds and 2 Belgian endangered local breeds. The cells showed all the characteristics of the PGCs in terms of morphology, markers' expression and gonadal migration. We also applied a cryopreservative vitrification method, which showed results superior to those obtained by conventional slow freezing. During the last part of this work, we genetically engineered PGCs to obtain stable transfectants expressing GFP. These transfectants allowed us to examine long-term gonadal colonization and germline transgene transmission. We also tested a Cre recombinase-mediated site-specific cassette exchange method. As a final result, we have developed the methods and know-how mandatory to allow efficient harvesting, expansion, genetic engineering, reimplantation and cryopreservation of chicken PGCs, and we have shown the possibility of germline transmission of a long term cultured and genetically modified PGCs line. [less ▲]

Detailed reference viewed: 77 (33 ULiège)
Full Text
Peer Reviewed
See detailCryopreservation of chicken primordial germ cells by vitrification and slow-freezing: a comparative study
Tonus, Céline ULiege; Connan, Delphine ULiege; Waroux, Olivier ULiege et al

in Theriogenology (2017), 88

In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed ... [more ▼]

In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed. Viability immediately after warming was close to 80% and did not differ between the two cryopreservation methods. Proliferation tended to be slower for both cryopreservation methods compared to controls, but the difference was significant only for vitrification. No difference was found between the two methods after flow cytometry analysis of SSEA-1 expression and RT-PCR on several factors related to PGCs phenotype. After one week in culture, all cryopreserved cells reached controls main morphological and expanding (viability/proliferation) features. However, slow freezing generated more unwanted cells clusters than vitrification. After injection of the PGCs into recipient embryos, vitrified PGCs showed a clear, yet not significant, tendency to colonize the gonad at a higher rate than slow frozen PGCs. Slow freezing in cryovials remains simple, inexpensive and less technically demanding than vitrification. Nevertheless, the intrinsic advantages of our aseptic vitrification method and the present study suggest that this should be considered as safer than classical slow freezing for cryopreserving chicken PGCs. [less ▲]

Detailed reference viewed: 167 (58 ULiège)
Full Text
See detailFormasup: Portfolio professionnel
Tonus, Céline ULiege

Master's dissertation (2016)

Detailed reference viewed: 38 (11 ULiège)
Full Text
Peer Reviewed
See detailLong term-cultured and cryopreserved primordial germ cells from various chicken breeds retain high proliferative potential and gonadal colonisation competency
Tonus, Céline ULiege; Cloquette, Karine; Ectors, Fabien ULiege et al

in Reproduction, Fertility and Development (2016), 28(5), 628-639

Detailed reference viewed: 186 (78 ULiège)
Full Text
See detailEquine cadaver ligaments : A new promising source of stem cells
Shikh Al Sook, Mohamad Khir ULiege; Gabriel, Annick ULiege; Salouci, Moustafa et al

Scientific conference (2015, November 07)

Detailed reference viewed: 171 (95 ULiège)
Full Text
See detailLong term culture, cryopreservation and genetic modification of chicken primordial germ cells
Tonus, Céline ULiege; Garcia Gil, Francisco José ULiege; Cloquette, Karine et al

Poster (2015, October 16)

Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties ... [more ▼]

Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties in vitro and are foreseen as promising tools for developing efficient avian genetic engineering and preservation of germplasm. We propose original methods that allow long term expansion, efficient cryopreservation and genetic modification of primary cultures of undifferentiated PGCs. PGCs are collected from embryonic blood during their migratory period and grown in cell-culture insert in the presence of feeder cells (BRL). This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of PGCs lines. Forty percent of blood samples gave rise to lines originating from three commercial layer and two Belgian endangered breeds. PGCs lines were characterized for the expression of the stem cells and PGCs marker SSEA-1 by FACS. RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. All lines were male although isolated from pooled male and female blood samples. Two cryopreservation methods were developed based upon slow-freezing and aseptic vitrification. Both have shown a similar effectiveness in allowing storage without phenotype drift. Stably expressing lines were obtained by Lipofectamine® mediated transfection of a GFP plasmid. PGCs were subsequently injected in recipient embryos. Persistence of exogenous PGCs in the developing gonad of recipient embryos confirmed that PGCs retain their gonadal colonisation ability, both after long-term culture and after cryopreservation. [less ▲]

Detailed reference viewed: 91 (17 ULiège)
Full Text
Peer Reviewed
See detailEquine cadaver ligaments : A new promising source of stem cells
Shikh Al Sook, Mohamad Khir ULiege; Gabriel, Annick ULiege; Salouci, Moustafa et al

Poster (2015)

Detailed reference viewed: 250 (74 ULiège)
Full Text
See detailCertificat d'université en Tutorat à Distance: portfolio de formation
Tonus, Céline ULiege

Master's dissertation (2014)

Detailed reference viewed: 35 (7 ULiège)
Full Text
See detailLong term culture and characterization of avian primordial germ cells
Waroux, Olivier ULiege; Tonus, Céline ULiege; Grobet, Luc ULiege

Conference (2013, October 26)

Detailed reference viewed: 37 (12 ULiège)
Full Text
Peer Reviewed
See detailLong term culture and characterization of chicken primordial germ cells
Tonus, Céline ULiege; Cloquette, Karine; Ectors, Fabien ULiege et al

Poster (2013, October)

Avian primordial germ cells (PGCs) can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species ... [more ▼]

Avian primordial germ cells (PGCs) can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species. We have developed original methods that allow long term (20 month) expansion of primary cultures of undifferentiated PGCs and their efficient cryopreservation. Blood samples were collected from stage 13-18 embryos, pooled, deposited in cell culture inserts and co-cultivated in the presence of irradiated BRL cells. This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of undifferentiated PGCs lines. Overall, 35% of blood samples gave rise to PGCs cell lines originating from three commercial layer breeds and two Belgian endangered breeds. Moreover, we recently isolate and cultivate a new PGC line from turkey. All PGCs lines were first characterised for the expression of the stem cells and PGCs characteristic marker SSEA-1 by FACS. RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. In addition, by means of a quantitative PCR amplification of a chromosome W specific sequence, we demonstrated a progressive drift of all our lines towards the male sex (WL), while they were initially isolated from pooled blood samples with statistically equivalent numbers of male and female embryos (35 females: 29 males). PGCs were subsequently efficiently cryopreserved by slow freezing or by a newly developed vitrification method. Labelled PGCs from 10 lines were injected in recipient embryos. At day 6, colonization of the genital ridges confirmed that PGCs retain their gonadal migratory ability, both after long-term culture (min 3, max 20 month) and after cryopreservation. In order to evaluate the germinal differentiation of cultured PGCs during the gonadal development as well as the germline transmission rate, we established a stably expressing GFP line that was successfully injected in emrbyos. Results are in progress. [less ▲]

Detailed reference viewed: 97 (6 ULiège)
Full Text
See detailA method allowing long term culture and expansion of chicken primordial germ cells
Tonus, Céline ULiege; Waroux, Olivier ULiege; Grobet, Luc ULiege

Conference (2013, March 12)

We developed an original method allowing long term culture (up to 20 month) of undifferentiated chicken PGCs, with good proliferation rates. We use a co-culture system: PGCs are cultivated in cell culture ... [more ▼]

We developed an original method allowing long term culture (up to 20 month) of undifferentiated chicken PGCs, with good proliferation rates. We use a co-culture system: PGCs are cultivated in cell culture inserts, in the presence of irradiated Buffalo Rat Liver cells. According to our own experience, physical separation of PGCs and feeder cells limits the differentiation of PGCs. Our PGCs lines keep their original phenotype including their gonadal colonization ability. We also developed a novel cryopreservation method based on vitrification, in complement of a slow rate freezing. [less ▲]

Detailed reference viewed: 36 (8 ULiège)
Full Text
Peer Reviewed
See detailLong term culture and characterization of chicken primordial germ cells
Tonus, Céline ULiege; Waroux, Olivier ULiege; Cloquette, Karine et al

Poster (2012, November)

Avian primordial germ cells (PGCs), can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species ... [more ▼]

Avian primordial germ cells (PGCs), can keep their germ cells properties and are foreseen as promising tools for developing avian transgenesis and preservation of genetic resources of endangered species. We have developed original methods that allow long term (20 month) expansion of primary cultures of undifferentiated PGCs and their efficient cryopreservation. Blood samples were collected from stage 13-18 embryos, pooled, deposited in cell culture inserts and co-cultivated in the presence of irradiated BRL cells. This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of undifferentiated PGCs lines. Overall, 35% of blood samples gave rise to PGCs cell lines originating from three commercial layer breeds and two Belgian endangered breeds. PGCs lines were first characterised for the expression of the stem cells and PGCs characteristic marker SSEA-1 by FACS (expression rate: 90-99%). RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. In addition, by means of a quantitative PCR amplification of a chromosome W specific sequence, we demonstrated a drift of all our lines towards the male sex (WL), while they were initially isolated from pooled blood samples with statistically equivalent numbers of male and female embryos (35 females: 29 males). PGCs were subsequently efficiently cryopreserved by slow freezing or by a newly developed vitrification method. Labelled PGCs from 10 lines were injected in recipient embryos. Colonization of the genital ridges confirmed that PGCs retain their gonadal migratory ability, both after long-term culture (min 3, max 20 month) and after cryopreservation. [less ▲]

Detailed reference viewed: 154 (27 ULiège)