References of "Sounouvou, Axel Gérald Hope Tognidé"
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See detailCymbopogon giganteus Chiov. essential oil: Direct effects or activity in combination with antibiotics against multi-drug resistant bacteria
Toukourou, Habib; Sounouvou, Axel Gérald Hope Tognidé ULiege; Catteau, Lucy et al

in Journal of Applied Biology and Biotechnology (2020), 8(1), 84-89

The discovery of new antimicrobial agents is necessary due to the emergence of multi-drug bacterial resistance. The aim of this work was to study the direct and indirect antimicrobial activity of a ... [more ▼]

The discovery of new antimicrobial agents is necessary due to the emergence of multi-drug bacterial resistance. The aim of this work was to study the direct and indirect antimicrobial activity of a Beninese sample of Cymbopogon giganteus essential oil (EOCG) on multi-drug resistant clinical bacteria, its chemical composition, and its cytotoxicity. Direct antimicrobial activity was tested by determination of minimal inhibitory concentration (MIC), and indirect activity, by determining Fractional Inhibitory Concentration Index using checkerboard [fractional inhibitory concentration indices (FICI); synergy: FICI ≤ 0.5; additivity: 0.5 < FICI ≤ 1]. EOCG composition was determined by GC-MS and GC-FID and cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphényltetrazolium bromide (MTT) assay. p-Menthane derivatives (54.87%) and limonene (12.07%) were detected as major compounds by GC analysis. Our results confirmed the direct antimicrobial activity of EOCG, but here on clinical resistant strains (MIC from 0.125% v/v to 0.5% v/v). We also show synergistic effects between EOCG and amoxicillin with FICI ranges of 0.12–0.5 against two Escherichia coli resistant clinical strains, synergistic to additive effects between EOCG and colistin or oxacillin/ampicillin, respectively, against Pseudomonas aeruginosa PA544 and Staphylococcus epidermidis SECN361 (two resistant clinical isolates). Our results also indicate that EOCG had low cytotoxicity (IC50: 67.06 ± 2.694 μg/ml). [less ▲]

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See detailDevelopment of new dermatological formulations for the treatment of cutaneous candidiasis
Zinsou, Aude; Assanhou, Gabin; Ganfon, Habib et al

in Scientific African (2020), 8

The inhibitory activity of the essential oils (EO) of mint and thyme on Candida albicans are well known, however, their valorization in a dosage form for the management of cutaneous candidiasis has been ... [more ▼]

The inhibitory activity of the essential oils (EO) of mint and thyme on Candida albicans are well known, however, their valorization in a dosage form for the management of cutaneous candidiasis has been little explored. This study aimed to formulate innovative and cost-effective dermatological topicals based on mint and thyme essential oils for the treatment of cutaneous candidiasis. Thyme essential oil was obtained commercially, while that of mint was obtained by hydrodistillation. In order to determine their antifungal activity, both essentials oils were tested alone and in combination against a reference strain of Candida albicans (strain MHMR) and clinical strains of Candida albicans and Candida tropicalis. Three creams were formulated using natural (shea butter, palm oil) and chemical (cetomacrogol) excipients. The efficacies of these creams were assessed in vivo using Wistar rats infected with Candida albicans MHMR. The in vitro antimicrobial study showed that C. albicans MHMR was more sensitive to thyme essential oil with a MIC of 310 μgmL−1, which was higher than the reference fluconazole used for the in vitro study (32 μgmL−1). The antimicrobial synergy study of both essential oils using checkerboard test demonstrated an additive effect of mint and thyme essential oils on C. albicans MHMR. GC/FID and GC/MS analyses led to the identification of thymol and menthol respectively as the main components of thyme and mint essential oils. A shea butter cream base and a shea butter and palm oil cream both containing 5% of EO (mint and thyme) exhibited the best in vivo antimicrobial activities, inducing optimal wound healing in infected rats compared with ketoconazole commercial cream used for the in vivo study. These results provide a solid basis at least in part, for the use of essential oil in creams formulated with natural excipients for the management of cutaneous candidiasis. [less ▲]

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See detailChromametric assessment of drug skin tolerance: A comparative study between Africans and Caucasians skins
Sounouvou, Axel Gérald Hope Tognidé ULiege; Lechanteur, Anna ULiege; Quetin-Leclercq, Joëlle et al

in Skin Research and Technology (2019)

Background/Aims: During dermatological forms development, one of the simplest non-invasive techniques used to evaluate cutaneous tolerance of formulations is to monitor the color changes using a ... [more ▼]

Background/Aims: During dermatological forms development, one of the simplest non-invasive techniques used to evaluate cutaneous tolerance of formulations is to monitor the color changes using a tristimulus chromameter. Most published tolerance studies involving chromametric measurements are performed on Caucasian subjects. However, in the context of drug formulation for African-type populations, it is not always relevant to transpose tolerance results obtained on Caucasians populations to African-type ones due to histological ethnic differences of the skin. The goal of this work was to assess whether tristimulus chromameter can be used to highlight color variations following the application of dermatological topics on black skin in order to validate skin tolerance studies made on African-type subjects. Materials and Methods: After application of two commercial creams with opposite side effects (skin irritation and skin blanching) in both Africans and Caucasians populations, color variations were evaluated using a tristimulus chromameter in L*a*b* color system and compared between both populations. L* indicating color brightness, a* represents green and red directions and b* represents blue and yellow directions. Results: While skin irritation resulted in a significant increase of a* parameter in both studied populations, the skin blanching resulted in a decrease of a* associated with an increase of L*. Conclusion: We established that tristimulus chromameter can be used to achieve in vivo skin tolerance study of dermatologic formulations in Africans despite their dark skin even though it appeared less sensitive. This study can speed up the development of dermatological forms dedicated to Africans and/or Caucasians subjects. [less ▲]

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See detailSilicones in development of a highly persistent sprayable emulsion containing essential oils for treatment of common skin infections
Sounouvou, Axel Gérald Hope Tognidé ULiege; Defourny, Charline; Quetin-Leclercq, Joëlle et al

Poster (2019, September)

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See detailAssessment of drug skin tolerance in Africans using a chromameter
Sounouvou, Axel Gérald Hope Tognidé ULiege; Quetin-Leclercq, Joëlle; Piel, Géraldine ULiege et al

Conference (2019, May 20)

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See detailAssessment of drug skin tolerance in Africans using chromameter
Sounouvou, Axel Gérald Hope Tognidé ULiege; Quetin-Leclercq, Joëlle; Piel, Géraldine ULiege et al

Poster (2019, March 26)

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See detailChemical composition, direct and indirect antibacterial activity against multidrug resistant bacteria and toxicity assessment of essential oil of Cymbopogon giganteus leaves
Toukourou, Habib; Sounouvou, Axel Gérald Hope Tognidé ULiege; Catteau, Lucy et al

in Planta Medica (2019)

Because of antibioresistance rising, the search of new alternative strategies by combining classic antibiotics and essentials oils to restore antibiotics efficacy may be a promising approach. The aim of ... [more ▼]

Because of antibioresistance rising, the search of new alternative strategies by combining classic antibiotics and essentials oils to restore antibiotics efficacy may be a promising approach. The aim of this work was to study the direct and indirect antimicrobial activity of Cymbopogon giganteus essential oil from Benin (EOCG) on multidrug resistant bacteria, its chemical composition and its oral acute toxicity. Direct antimicrobial activity was tested by determination of Minimal Inhibitory Concentration (MIC), and indirect activity, by calculating the Fractional Inhibitory Concentration Index using checkerboard (FICI; synergy: FICI ≤ 0.5; additivity: 0.5<FICI≤1) on reference but also on multidrug resistant clinical isolates. Composition was determined by GC-MS and GC-FID. Cytotoxicity was evaluated in vitro against human non-cancer fibroblast cell line (WI38) by MTT assay and oral acute toxicity by determination of the “limit dose test” at 2000mg/kg. Limonene (12.07%) and p-menthane derivatives (54.87%) were the major components. Our results confirmed the direct antimicrobial activity of EOCG, but here on clinical resistant strains (MIC from 0.125%v/v to 0.5%v/v). We also observed, for the first time, the synergistic effects between EOCG and amoxicillin with FICI between 0.12-0.5 against two Escherichia coli amoxicillin-resistant clinical strains, synergistic to additive effects between EOCG and colistin or oxacillin/ampicillin respectively against Pseudomonas aeruginosa PA544 and Staphylococcus epidermidis SE361 (two multiresistant clinical isolates). EOCG had a low cytotoxicity (IC50: 67.06±2.69 μg/ml) and no acute toxicity at the dose of 2000mg/kg per os. This is the first report of oral acute toxicity assessment of this essential oil. [less ▲]

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See detailPilot study of quality control of Artemisia annua-based herbal medicine sold in Benin
Ganfon, Habib; Yemoa, Achille; Assanhou, Cabin et al

in Journal of Pharmacognosy and Phytochemistry (2019), 8(6), 1817-1822

The quality control of medicines is one of the key factors which determine its efficacy. In Africa, particularly in Benin, a large population often uses traditional medicines. Therefore, controlling their ... [more ▼]

The quality control of medicines is one of the key factors which determine its efficacy. In Africa, particularly in Benin, a large population often uses traditional medicines. Therefore, controlling their quality becomes a major concern for public health. It is in this context that this study was designated to ensure an adequate system of drugs quality control, especially herbal medicines. We collected three different samples (T1, T2 and T3) of Traditional Medicines (MTA) based on Artemisia annua and we controlled their quality. The identification of artemisinin was carried out by Thin Layer Chromatography (TLC) and the quantification by High Performance Liquid Chromatography (HPLC). After analyzing the organoleptic characteristic, the loss of water and the impurities, we found that only T3 was conform to all the specification as MTA. This work opens a new platform of expertise in the field for our team and further a hope for the management of public health in term of the control of MTA. [less ▲]

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See detailDevelopment of phtalate analysis methodology by high performance liquid chromatography coupled with diode array detector (HPLC/DAD)
Ossoulanan, Adéwolé Jules; Sounouvou, Axel Gérald Hope Tognidé ULiege; Kassehin, Urbain et al

in Elixir International Journal (2018), (118), 50779-50784

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See detailSynthesis and toxicity assessment of druggable thiosemicarbazones
Sounouvou, Axel Gérald Hope Tognidé ULiege; Kassehin, Urbain Comlan; Gbaguidi, Fernand A et al

in World Journal of Traditional Chinese Medicine (2015), 1(4), 103

Detailed reference viewed: 32 (11 ULiège)