References of "Shimomura, K"
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See detailAnthemis nobilis L. (roman chamomille): in vitro culture, micropropagation, and the production of essential oil.
Fauconnier, Marie-Laure ULiege; Jaziri, Mondher; Homes, Jacques et al

in Bajaj, Y. P. S. (Ed.) Medicinal and aromatic plants IX (1996)

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See detail- Establishment of normal and transformed root cultures of Artemisia annua L. for artemisinin production.
Jaziri, M.; Shimomura, K.; Yoshimatsu, K. et al

in Journal of Plant Physiology (1995), 145(1-2), 175-177

Transformed cultures of Artemisia annua L. (Asteraceae) were established by the co-culture method using leaf segments of A. annua and Agrobacterium rhizogenes NCIB 8196 or MAFF 03-01724. The hairy root ... [more ▼]

Transformed cultures of Artemisia annua L. (Asteraceae) were established by the co-culture method using leaf segments of A. annua and Agrobacterium rhizogenes NCIB 8196 or MAFF 03-01724. The hairy root clones thus obtained grew vigorously on hormone-free medium, showing the typical transformed morphology. The genetic transformation of the root was proved by the opine assay. Normal root and shoot cultures were also established. A highly specific and sensitive enzyme-linked immuno-sorbent assay (ELISA) method was used for the detection and semi-quantitative determination of artemisinin and structurally related compounds in these cultures. The presence of artemisinin was confirmed by gas chromatography coupled to mass spectrometry (GC-MS) analysis. The hairy roots cultured in the dark produced no detectable level of artemisinin as shown by the adventitious shoots cultured under light conditions. The ELISA analysis of the green hairy roots cultured in liquid medium under a 16 h light/day photoperiod showed the existence of compound(s) structurally related to artemisinin, though normal and hairy roots cultured in the dark give no detectable levels of immuno-signal. [less ▲]

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See detailTerpenoids production by normal and transformed cultures of Artemisia annua L.
Jaziri, M.; Shimomura, K.; Yoshimatsu, K. et al

Poster (1993, August 31)

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See detailEssential oil production by Anthemis nobilis L. tissue culture.
Fauconnier, Marie-Laure ULiege; Jaziri, M.; Marlier, Michel et al

in Journal of Plant Physiology (1993), 141(6), 759-761

The production of essential oil by tissue culture s ol Antbemis nobilis L. including cell suspension, shoot and crown-gall cultures is reported. The biosynthetic capability of these different cultures was ... [more ▼]

The production of essential oil by tissue culture s ol Antbemis nobilis L. including cell suspension, shoot and crown-gall cultures is reported. The biosynthetic capability of these different cultures was compared with that of plants grown in a field. The addition of crude polysaccharide fraction prepared from yeast extract and from the plant itself to shoot cultures affected the composition and total essential content (from 0.08 o/o to 0.3Qo/o dry weight). The essential oil content of the crown-gall tissue ol A. nobilis was 0.25 % of the dry weight and the composition of the essential oil was comparable to that of the flowers. [less ▲]

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