References of "Sanchez, Christelle"
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See detailFib3-3 as a biomarker for osteoarthritis in a rat model with metabolic dysregulation
de Visser, Huub; Sanchez, Christelle ULiege; Mastbergen, Simon et al

in Cartilage (in press)

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See detailA systematic review of the secretome of skeletal muscle cells
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

Poster (2019, May 03)

This poster presents the preliminary results of a systematic review that summarized recent mass-spectrometry based proteomics discoveries of the secretome of skeletal muscle cells in response to disease ... [more ▼]

This poster presents the preliminary results of a systematic review that summarized recent mass-spectrometry based proteomics discoveries of the secretome of skeletal muscle cells in response to disease, exercise or metabolic stress. [less ▲]

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See detailSécrétome des cellules musculaires squelettiques: revue systématique de la littérature
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

Conference (2019, April 04)

Présentation des résultats préliminaires d'une revue systématique en cours de rédaction portant sur le sécrétome des cellules musculaires squelettiques. L'étude du sécrétome de ces cellules pourrait ... [more ▼]

Présentation des résultats préliminaires d'une revue systématique en cours de rédaction portant sur le sécrétome des cellules musculaires squelettiques. L'étude du sécrétome de ces cellules pourrait permettre de comprendre les mécanismes liés au vieillissement mais également de permettre la découverte de biomarqueurs et de cibles thérapeutiques de certaines maladies [less ▲]

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See detailSyndecan-4 is increased in osteoarthritic knee, but nor ip or shoulder, articular hypertrophic chondrocytes
Sanchez, Christelle ULiege; Lambert, Cécile ULiege; Dubuc, Jean-Emile et al

in Osteoarthritis and Cartilage (2019, April), 27(Suppl 1), 159-160

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See detailThe secretome of skeletal muscle cells: A systematic review
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

Poster (2019, February 20)

This poster presents the preliminary results of a systematic review that summarized recent mass-spectrometry based proteomics discoveries of the secretome of skeletal muscle cells in response to disease ... [more ▼]

This poster presents the preliminary results of a systematic review that summarized recent mass-spectrometry based proteomics discoveries of the secretome of skeletal muscle cells in response to disease, exercise or metabolic stress. [less ▲]

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See detailLe syndécan-4 est un marqueur de l’hypertrophie des chondrocytes du genou arthrosique
Sanchez, Christelle ULiege; Lambert, Cécile ULiege; Dubuc, JE et al

in Revue du Rhumatisme (2018, November), 85S

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See detailProteomic analysis of osteoblasts secretome provides new insights in mechanisms underlying osteoarthritis subchondral bone sclerosis
Sanchez, Christelle ULiege; Mazzucchelli, Gabriel ULiege; Comblain, Fanny et al

in Annals of the Rheumatic Diseases (2018, June), 77(supplement 2), 894

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See detailComparison of secretome from osteoblasts derived from sclerotic versus non-sclerotic subchondral bone in OA: A pilot study.
Sanchez, Christelle ULiege; Mazzucchelli, Gabriel ULiege; Lambert, Cécile ULiege et al

in PLoS ONE (2018), 13(3), 0194591

OBJECTIVE: Osteoarthritis (OA) is characterized by cartilage degradation but also by other joint tissues modifications like subchondral bone sclerosis. In this study, we used a proteomic approach to ... [more ▼]

OBJECTIVE: Osteoarthritis (OA) is characterized by cartilage degradation but also by other joint tissues modifications like subchondral bone sclerosis. In this study, we used a proteomic approach to compare secretome of osteoblast isolated from sclerotic (SC) or non sclerotic (NSC) area of OA subchondral bone. DESIGN: Secretome was analyzed using differential quantitative and relative label free analysis on nanoUPLC G2 HDMS system. mRNA of the more differentially secreted proteins were quantified by RT-PCR in cell culture from 5 other patients. Finally, osteomodulin and fibulin-3 sequences were quantified by western blot and immunoassays in serum and culture supernatants. RESULTS: 175 proteins were identified in NSC osteoblast secretome. Data are available via ProteomeXchange with identifier PXD008494. Compared to NSC osteoblast secretome, 12 proteins were significantly less secreted (Osteomodulin, IGFBP5, VCAM-1, IGF2, 78 kDa glucose-regulated protein, versican, calumenin, IGFBP2, thrombospondin-4, periostin, reticulocalbin 1 and osteonectin), and 13 proteins were significantly more secreted by SC osteoblasts (CHI3L1, fibulin-3, SERPINE2, IGFBP6, SH3BGRL3, SERPINE1, reticulocalbin3, alpha-2-HS-glycoprotein, TIMP-2, IGFBP3, TIMP-1, SERPINF1, CSF-1). Similar changes in osteomodulin, IGF2, SERPINE1, fibulin-3 and CHI3L1 mRNA levels were observed. ELISAs assays confirm the decrease by half of osteomodulin protein in SC osteoblasts supernatant compared to NSC and in OA patients serum compared to healthy subjects. Fibulin-3 epitopes Fib3-1, Fib3-2 and Fib3-3 were also increased in SC osteoblasts supernatant compared to NSC. CONCLUSIONS: We highlighted some proteins differentially secreted by the osteoblasts coming from OA subchondral bone sclerosis. These changes contribute to explain some features observed in OA subchondral bone, like the increase of bone remodeling or abnormalities in bone matrix mineralization. Among identified proteins, osteomodulin was found decreased and fibulin-3 increased in serum of OA patients. These findings suggest that osteomodulin and fibulin-3 fragments could be biomarkers to monitor early changes in subchondral bone metabolism in OA. [less ▲]

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See detailCross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment
Elango, Jeevithan; Sanchez, Christelle ULiege; de Val, José Eduardo Maté Sánchez et al

in Scientific Reports (2018), 8(1), 5318

Homeostasis of osteoclast formation from bone marrow macrophages (BMM) is regulated by paracrine signals of the neighbourhood bone cells particularly mesenchymal stem cells (MSC), osteoblasts and ... [more ▼]

Homeostasis of osteoclast formation from bone marrow macrophages (BMM) is regulated by paracrine signals of the neighbourhood bone cells particularly mesenchymal stem cells (MSC), osteoblasts and osteocytes (OC). Besides paracrine cues, collagen and glycosaminoglycan are involved in controlling bone homeostasis. Towards this approach, different molecular weight collagens were reacted with MSC, OC and BMM to understand the bone homeostasis activity of collagen. The up-regulating effect of collagens on osteogenic cell growth was confirmed by the presence of mineralized nodules in the osteoblastogenic lineage cells and increased osteogenic stimulatory gene expression. The decreased BMM-derived TRAP+ osteoclasts number and osteoclastogenic regulatory gene expression of OC could demonstrate the exploitive osteoclastogenic activity of collagens. Osteoclastogenesis from BMM was triggered by paracrine cues of OC in some extend, but it was down-regulated by collagen. Overall, the effect of collagen on osteoclastogenesis and osteoblastogenesis may depend on the molecular weight of collagens, and collagen suppresses osteoclastogenesis, at least in part by downregulating the secretion of cytokines in OC. [less ▲]

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See detailFib3-3 as a Biomarker for Osteoarthritis in a Rat Model with Metabolic Dysregulation.
de Visser, Huub M.; Sanchez, Christelle ULiege; Mastbergen, Simon C. et al

in Cartilage (2018)

Objective Fibulin-3 is a glycoprotein highly expressed in osteoarthritic cartilage and inhibits angiogenesis and chondrocyte differentiation. Recent studies have indicated that fibulin-3 has potential ... [more ▼]

Objective Fibulin-3 is a glycoprotein highly expressed in osteoarthritic cartilage and inhibits angiogenesis and chondrocyte differentiation. Recent studies have indicated that fibulin-3 has potential value as a biomarker in osteoarthritis. The aim of the present study is to examine the role of 3 fibulin-3 peptides (Fib3-1, Fib3-2, and Fib3-3) and a type II collagen degradation product in a rat osteoarthritis model with systemic metabolic alterations combined with local cartilage damage. Design Forty, 12-week-old male, Wistar rats were randomly divided over 2 groups: a standard or a high-fat diet inducing metabolic dysregulation. After 12 weeks, articular cartilage damage was induced on the femoral condyles (groove model), in 1 knee joint in 14 rats of each diet group. At endpoint, blood was collected and serum was isolated. Enzyme-linked immunosorbent assay on all selected fibulin-3 fragments was performed from serum samples in addition to immunohistochemical analysis for Fib3-3. Results Serum concentrations of Fib3-3 were increased by 29.9%, when cartilage damage was induced in addition to a high-fat diet. Fib3-3 was also associated with an increased histological total joint degeneration ( r = 0.435) and cartilage degeneration ( r = 0.435). Immunostainings demonstrated increased Fib3-3 in the superficial cartilage of animals with high-fat diet and/or cartilage damage. Conclusions In the rat groove model combined with high-fat diet-induced metabolic dysregulation an increased Fib3-3 concentration was observed systemically, which is associated with local joint degeneration. This suggests that systemic Fib3-3 concentrations can indicate the status of joint degeneration and function as a biomarker in osteoarthritis. [less ▲]

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See detailWP1 - A summary
Sanchez, Christelle ULiege

Conference (2017, September 20)

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See detailChondrocyte secretome: a source of novel insights and exploratory biomarkers of osteoarthritis
Sanchez, Christelle ULiege; Bay-Jensen, Anne-Christine; Pap, Thomas et al

in Osteoarthritis and Cartilage (2017), 25

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See detailStudy of the evolution of the osteoarthritis pathology and the mechanical properties of cartilage in a spontaneous osteoarthritis model in the Dunkin-Hartley guinea pigs.
Legrand, Catherine ULiege; Centonze, Prescilia ULiege; Comblain, Fanny ULiege et al

Poster (2017, April 27)

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes ... [more ▼]

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes. In parallel to these histological analyzes, we studied the mechanical properties of cartilage at different stages of disease progression in the Dunkin-Hartley guinea pigs. We also correlated the severity of histological lesions with the mechanical properties of cartilage. [less ▲]

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See detailStudy of the evolution of the osteoarthritis pathology and the mechanical properties of cartilage in a spontaneous osteoarthritis model in the Dunkin-Hartley guinea pigs.
Legrand, Catherine ULiege; Centonze, Prescilia ULiege; Comblain, Fanny ULiege et al

in Osteoarthritis and Cartilage (2017, April), 25

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan con- tent, the integrity of the tidemark, the cellularity, and osteophytes ... [more ▼]

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan con- tent, the integrity of the tidemark, the cellularity, and osteophytes. In parallel to these histological analyzes, we studied the mechanical properties of cartilage at different stages of disease progression in the Dunkin-Hartley guinea pigs. We also correlated the severity of histo- logical lesions with the mechanical properties of cartilage. [less ▲]

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See detailEffect of moderate increasing exercice on the mechanical balance of the knee joint in young rats
Rahnamay Moshtagh, Parisa; Korthagen, N; Plomp, S et al

Conference (2017)

Detailed reference viewed: 16 (2 ULiège)