References of "Remacle, Claire"
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See detailCharacterization of the GPR1/FUN34/YaaH protein family in the green microalga Chlamydomonas suggests their role as intracellular membrane acetate channels
Durante, Lorenzo ULiege; Hübner, Wolfgang; Lauersen, Kyle J et al

in Plant Direct (2019), 3(6),

The unicellular green microalga Chlamydomonas reinhardtii is a powerful photosynthetic model organism which is capable of heterotrophic growth on acetate as a sole carbon source. This capacity has enabled ... [more ▼]

The unicellular green microalga Chlamydomonas reinhardtii is a powerful photosynthetic model organism which is capable of heterotrophic growth on acetate as a sole carbon source. This capacity has enabled its use for investigations of perturbations in photosynthetic machinery as mutants can be recovered heterotrophically. Fixation of acetate into cellular carbon metabolism occurs first by its conversion into acetyl‐CoA by a respective synthase and the generation of succinate by the glyoxylate cycle. These metabolic steps have been recently determined to largely occur in the peroxisomes of this alga; however, little is known about the trafficking and import of acetate or its subcellular compartmentalization. Recently, the genes of five proteins belonging to the GPR1/FUN34/YaaH (GFY) superfamily were observed to exhibit increased expression in C. reinhardtii upon acetate addition, however, no further characterization has been reported. Here, we provide several lines of evidence to implicate CrGFY1–5 as channels which share structural homology with bacterial succinate‐acetate channels and specifically localize to microbodies, which are surprisingly distinct from the glyoxylate cycle‐containing peroxisomes. We demonstrate structural models, gene expression profiling, and in vivo fluorescence localization of all five isoforms in the algal cell to further support this role. [less ▲]

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See detailCharacterization of fermentation pathways and their interaction with photosynthesis in different microalgae
Gain, Gwenaëlle ULiege; Berne, Nicolas; Remacle, Claire ULiege et al

Poster (2019, February 07)

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See detailAcetate assimilation in the microalga Chlamydomonas
Remacle, Claire ULiege

Scientific conference (2019, January 17)

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See detailAcetate metabolism in the unicellular green microalga Chlamydomonas
Remacle, Claire ULiege

Conference (2018, November 27)

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See detailAnalysis of acetate assimilation pathways in Chlamydomonas cells cultivated in the light
Remacle, Claire ULiege

Scientific conference (2018, November 12)

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See detailThe GPR1/FUN34/YaaH(/SatP) members in Chlamydomonas reinhardtii, are they novel peroxisomal transporters?
Durante, Lorenzo ULiege; Lauersen, Kyle J.; Hübner, Wolfgang et al

Poster (2018, June 17)

The green microalga C. reinhardtii can grow heterotrophically in presence of acetate in the dark as sole carbon source, due to the anaplerotic pathway of glyoxylate cycle1 almost completely localised in ... [more ▼]

The green microalga C. reinhardtii can grow heterotrophically in presence of acetate in the dark as sole carbon source, due to the anaplerotic pathway of glyoxylate cycle1 almost completely localised in the peroxisomal microbodies2 . Despite the significant advances gained on carbon metabolism, the genes involved in acetate transport are not yet identified. Recently, five functionally uncharacterized members predicted to have transporter-like structure were classified within the protein family GPR1/FUN34/YaaH(/SatP) (GFY)3, which comprises genes coding for putative carboxylic acids transporters in other microorganisms, including acetate as substrate4. In C. reinhardtii, GFY1-5 as we shown by YFP fusion protein, localise to peroxisomal membranes. In addition, we also showed that these genes present distinct expression patterns in different cultivation conditions, which correlates with associated co-expressed genes found in the databases. Therefore, although these proteins share between 78 and 97% similarity between their amino acid sequences, this might suggest non-redundant roles. To gain insight on their role, we are currently analysing insertional and knock-down mutants. Mutants for the genes GFY1, GFY2 and GFY3 were obtained from the Chlamydomonas Library Project 5. Mutants for GFY4, GFY5 and all GFY genes are presently under generation via artificial micro RNA. In conclusion, GFY members could represent different protagonists in transporting substrates in/out of peroxisomal microbodies, as putative transporters/permeases function. [less ▲]

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See detailRegulation of mitochondrial respiration by modulating calcium fluxes in the green algae Chlamydomonas reinhardtii
Fattore, Nicolo; Remacle, Claire ULiege; Morosinotto, Tomas et al

Poster (2018, May)

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See detailOxidative phosphorylation supercomplexes and respirasome reconstitution of the colorless alga Polytomella sp
Miranda Astudillo, Héctor Vicente ULiege; Colina-Tenorio, Lilia; Jiménez-Suárez, Alejandra et al

in Biochimica et Biophysica Acta. Bioenergetics (2018), 1859

The proposal that the respiratory complexes can associate with each other in larger structures named super- complexes (SC) is generally accepted. In the last decades most of the data about this ... [more ▼]

The proposal that the respiratory complexes can associate with each other in larger structures named super- complexes (SC) is generally accepted. In the last decades most of the data about this association came from studies in yeasts, mammals and plants, and information is scarce in other lineages. Here we studied the su- pramolecular association of the F1FO-ATP synthase (complex V) and the respiratory complexes I, III and IV of the colorless alga Polytomella sp. with an approach that involves solubilization using mild detergents, n-dodecyl-β-D- maltoside (DDM) or digitonin, followed by separation of native protein complexes by electrophoresis (BN- PAGE), after which we identified oligomeric forms of complex V (mainly V2 and V4) and different respiratory supercomplexes (I/IV6, I/III4, I/IV). In addition, purification/reconstitution of the supercomplexes by anion exchange chromatography was also performed. The data show that these complexes have the ability to strongly associate with each other and form DDM-stable macromolecular structures. The stable V4 ATPase oligomer was observed by electron-microscopy and the association of the respiratory complexes in the so-called “respirasome” was able to perform in-vitro oxygen consumption. [less ▲]

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See detailRegulation of mitochondrial respiration by modulating calcium fluxes in the green algae Chlamydomonas reinhardtii.
Fattore, Nicolo; Remacle, Claire ULiege; Morosinotto, Tomas et al

Conference (2018)

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See detailRNA-seq data and surprisal analysis of icl mutant and control strain of the green microalga Chlamydomonas reinhardtii during day/night cycles
Willamme, R.; Bogaert, K. A.; Remacle, Françoise ULiege et al

in Data in Brief (2018), 21

The data presented in this article are associated to the research article “Surprisal analysis of the transcriptomic response of the green microalga Chlamydomonas to the addition of acetate during day ... [more ▼]

The data presented in this article are associated to the research article “Surprisal analysis of the transcriptomic response of the green microalga Chlamydomonas to the addition of acetate during day/night cycles” (Willamme et al., 2018) [1]. Here the RNA-seq data of the icl mutant, a null mutant of the isocitrate lyase gene, and its control are summarized and the FPKM values are listed. The data were analysed using surprisal analysis and the genes contributing the strongest to the mutant and wild type phenotype are listed. The raw data are accessible at BioProject PRJNA437393 with SRA accession number SRP136101 (experiments SRX3824204–SRX3824249). The raw data set and expression values used for surprisal analysis are made public to enable critical or extended analyses. © 2018 The Authors [less ▲]

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See detailHost-pathogen biotic interactions shaped vitamin K metabolism in Archaeplastida
Cenci, U.; Qiu, H.; Pillonel, T. et al

in Scientific Reports (2018), 8(1),

Menaquinone (vitamin K2) shuttles electrons between membrane-bound respiratory complexes under microaerophilic conditions. In photosynthetic eukaryotes and cyanobacteria, phylloquinone (vitamin K1 ... [more ▼]

Menaquinone (vitamin K2) shuttles electrons between membrane-bound respiratory complexes under microaerophilic conditions. In photosynthetic eukaryotes and cyanobacteria, phylloquinone (vitamin K1) participates in photosystem I function. Here we elucidate the evolutionary history of vitamin K metabolism in algae and plants. We show that Chlamydiales intracellular pathogens made major genetic contributions to the synthesis of the naphthoyl ring core and the isoprenoid side-chain of these quinones. Production of the core in extremophilic red algae is under control of a menaquinone (Men) gene cluster consisting of 7 genes that putatively originated via lateral gene transfer (LGT) from a chlamydial donor to the plastid genome. In other green and red algae, functionally related nuclear genes also originated via LGT from a non-cyanobacterial, albeit unidentified source. In addition, we show that 3–4 of the 9 required steps for synthesis of the isoprenoid side chains are under control of genes of chlamydial origin. These results are discussed in the light of the hypoxic response experienced by the cyanobacterial endosymbiont when it gained access to the eukaryotic cytosol. © 2018, The Author(s). [less ▲]

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See detailInsights into the respiratory chain and oxidative stress
Larosa, Véronique ULiege; Remacle, Claire ULiege

in Bioscience Reports (2018)

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See detailSurprisal analysis of the transcriptomic response of the green microalga Chlamydomonas to the addition of acetate during day/night cycles
Willamme, R.; Bogaert, K. A.; Remacle, Françoise ULiege et al

in Chemical Physics (2018)

Our study aims to find gene pathways that depend on acetate assimilation under diurnal conditions in the microalga Chlamydomonas. We compare the transcriptome of two strains, one control and one mutant ... [more ▼]

Our study aims to find gene pathways that depend on acetate assimilation under diurnal conditions in the microalga Chlamydomonas. We compare the transcriptome of two strains, one control and one mutant deficient for the glyoxylate cycle essential in acetate metabolism, cultivated under day/night cycles with acetate. We apply surprisal analysis, an information-theoretic approach, to the RNA-seq data. Carrying out the analysis on groups of dark and light phase samples separately allows identifying constraints and gene pathways that discriminate between mutant and control samples. Carbon metabolism is the most important in the light phase for the control strain while the dark phase is enriched in cell division pathways. The mutant phenotype includes genes pathways of stress response and autophagy in the two phases. Cell division pathways are found in the light phase and catabolic pathways in the dark phase, highlighting a rewiring of the mutant transcriptome in these cyclic cultivation conditions. [less ▲]

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See detailRegulation of mitochondrial respiration by modulating calcium fluxes in the green algae Chlamydomonas reinhardtii.
Fattore, Nicolo; Remacle, Claire ULiege; Morosinotto, Tomas et al

Poster (2018)

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See detailSurprisal analysis of genome-wide transcript profiling identifies differentially expressed genes and pathways associated with four growth conditions in the microalga Chlamydomonas
Bogaert, K. A.; Manoharan-Basil, S. S.; Perez, E. et al

in PLoS ONE (2018), 13(4),

The usual cultivation mode of the green microalga Chlamydomonas is liquid medium and light. However, the microalga can also be grown on agar plates and in darkness. Our aim is to analyze and compare gene ... [more ▼]

The usual cultivation mode of the green microalga Chlamydomonas is liquid medium and light. However, the microalga can also be grown on agar plates and in darkness. Our aim is to analyze and compare gene expression of cells cultivated in these different conditions. For that purpose, RNA-seq data are obtained from Chlamydomonas samples of two different labs grown in four environmental conditions (agar@light, agar@dark, liquid@light, liquid@dark). The RNA seq data are analyzed by surprisal analysis, which allows the simultaneous meta-analysis of all the samples. First we identify a balance state, which defines a state where the expression levels are similar in all the samples irrespectively of their growth conditions, or lab origin. In addition our analysis identifies additional constraints needed to quantify the deviation with respect to the balance state. The first constraint differentiates the agar samples versus the liquid ones; the second constraint the dark samples versus the light ones. The two constraints are almost of equal importance. Pathways involved in stress responses are found in the agar phenotype while the liquid phenotype comprises ATP and NADH production pathways. Remodeling of membrane is suggested in the dark phenotype while photosynthetic pathways characterize the light phenotype. The same trends are also present when performing purely statistical analysis such as K-means clustering and differentially expressed genes. © 2018 Bogaert et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. [less ▲]

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See detailDispositif pour la production d'hydrogène gazeux
Lorge, Philippe ULiege; Remacle, Claire ULiege; Gerin, Stéphanie ULiege et al

Patent (2017)

Dispositif de dissociation d’une phase aqueuse pour produire de l’hydrogène gazeux, ledit dispositif comprenant : - une première zone comprenant ladite phase aqueuse, - un moyen de capture d’électrons ... [more ▼]

Dispositif de dissociation d’une phase aqueuse pour produire de l’hydrogène gazeux, ledit dispositif comprenant : - une première zone comprenant ladite phase aqueuse, - un moyen de capture d’électrons, - un moyen de capture de protons, et - une source énergétique, ledit dispositif comprenant une deuxième zone non aqueuse et étant conçu pour réaliser ladite réaction de réduction au niveau de ladite deuxième zone non aqueuse. [less ▲]

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See detailAnalysis of acetate assimilation pathways in Chlamydomonas cells cultivated in the light
Remacle, Claire ULiege

Conference (2017, September 25)

Detailed reference viewed: 18 (2 ULiège)
See detailRegulation of mitochondrial respiration by modulating calcium fluxes in the green algae Chlamydomonas reinhardtii
Fattore, Nicolo; Remacle, Claire ULiege; Morosinotto, Tomas et al

Poster (2017, September 04)

Detailed reference viewed: 61 (4 ULiège)
See detailA mitochondrial mutation increases high light resistance in Chlamydomonas reinhardtii.
Larosa, Véronique ULiege; Meneghesso, Andrea; La Rocca, Nicoletta et al

Poster (2017, September)

Detailed reference viewed: 14 (2 ULiège)