References of "RIZZUTO, R"
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See detailPhysiological characterization of a plant mitochondrial Calcium uniporter in Vitro and in Vivo
Teardo, E.; Carraretto, L.; Wagner, S. et al

in Plant Physiology (2017), 173(2), 1355-1370

Over the recent years, several proteins that make up the mitochondrial calcium uniporter complex (MCUC) mediating Ca2+ uptake into the mitochondrial matrix have been identified in mammals, including the ... [more ▼]

Over the recent years, several proteins that make up the mitochondrial calcium uniporter complex (MCUC) mediating Ca2+ uptake into the mitochondrial matrix have been identified in mammals, including the channel-forming protein MCU. Although six MCU gene homologs are conserved in the model plant Arabidopsis (Arabidopsis thaliana) in which mitochondria can accumulate Ca2+, a functional characterization of plant MCU homologs has been lacking. Using electrophysiology, we show that one isoform, AtMCU1, gives rise to a Ca2+-permeable channel activity that can be observed even in the absence of accessory proteins implicated in the formation of the active mammalian channel. Furthermore, we provide direct evidence that AtMCU1 activity is sensitive to the mitochondrial calcium uniporter inhibitors Ruthenium Red and Gd3+, as well as to the Arabidopsis protein MICU, a regulatory MCUC component. AtMCU1 is prevalently expressed in roots, localizes to mitochondria, and its absence causes mild changes in Ca2+ dynamics as assessed by in vivo measurements in Arabidopsis root tips. Plants either lacking or overexpressing AtMCU1 display root mitochondria with altered ultrastructure and show shorter primary roots under restrictive growth conditions. In summary, our work adds evolutionary depth to the investigation of mitochondrial Ca2+ transport, indicates that AtMCU1, together with MICU as a regulator, represents a functional configuration of the plant mitochondrial Ca2+ uptake complex with differences to the mammalian MCUC, and identifies a new player of the intracellular Ca2+ regulation network in plants. © 2017 American Society of Plant Biologists. All rights reserved. [less ▲]

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See detailPERK IS REQUIRED AT THE ER-TO-MITOCHONDRIA CONTACT SITES TO CONVEY APOPTOSIS FOLLOWING ROS-MEDIATED ER STRESS
VERFAILLIE, T; RUBIO, N; GARG, A et al

in Cell Death & Differentiation (2012), 19

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