References of "PINCEMAIL, Joël"
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See detailPAOT-Liquid® Technology: An easy Electrochemical Method for Evaluating Antioxidant Capacity of Wines
PINCEMAIL, Joël ULiege; Kaci, Mouna-Messaouda; Kevers, Claire ULiege et al

in Diseases (2019), 7(10),

Polyphenol compounds present in high quantity in wines are well-known to have potent cardio-protective properties through several biological mechanisms including antioxidant activity [1]. A large number ... [more ▼]

Polyphenol compounds present in high quantity in wines are well-known to have potent cardio-protective properties through several biological mechanisms including antioxidant activity [1]. A large number of methods have been developed in the laboratory for evaluating this last mechanism in food matrices. Most of them have, however, the disadvantage of being time consuming and require specific analytical protocols and devices. In the present study, we present the electrochemical PAOT (Pouvoir Antioxydant Total)-Liquid® Technology which can be easily used by winemakers for evaluating antioxidant activity during the wine process. The methodology is based on the measurement of electric potential variation resulting from chemical reactions between wine polyphenols and a free radical mediator M• as source of oxidants. Total antioxidant activity as estimated by the PAOT-Liquid® activity was 6.8 fold higher in red wines (n = 14) when compared to rosé (n = 3) and white (n = 3) wines built in a commercial market. Moreover, PAOT-Liquid® activity was highly correlated with total polyphenols content (TPC) of all wines (r = 0.9540, p < 0.0001) and the classical DPPH (2,2-diphenyl-1-picryhydrazyl) assay very which is often used for evaluating antioxidant capacity of food matrices (r = 0.9102, p < 0.0001). [less ▲]

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See detailEx-vivo vasorelaxation induced by polyphenol-rich extracts from plant, fruits and berries on porcine coronary artery rings
Ebabe Elle, Etienne ULiege; Defraigne, Jean-Olivier ULiege; Schini-Kerth, Valérie et al

in Free Radical Biology and Medicine (2018, June), 120

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See detailProcessing effects on antioxidant, glucosinolate and sulforaphane contents in broccoli and red cabbage
Tabart, Jessica ULiege; PINCEMAIL, Joël ULiege; Kevers, Claire ULiege et al

in European Food Research and Technology (2018), 244

The objective of this study is to investigate the effects of three home cooking methods traditionally used all around the world (boiling, steaming and microwaving) in two vegetables: broccoli and red ... [more ▼]

The objective of this study is to investigate the effects of three home cooking methods traditionally used all around the world (boiling, steaming and microwaving) in two vegetables: broccoli and red cabbage. Their effects on phytochemical content (i.e., polyphenols, ascorbic acid, anthocyanins, glucosinolates and sulforaphane) and on total antioxidant capacity were investigated. Steaming and microwaving were explored to understand the effect of cooking time and/or cooking power. Nutrient and health-promoting compounds in broccoli and red cabbage are significantly affected by domestic cooking. The boiling seems to result in a very significant loss of nutritional compounds by leaching in cooking water. However, steaming and microwaving allowed the preservation of the higher quantities of bioactive compounds such as antioxidant compounds and glucosinolates. Microwave cooking significantly influenced the concentrations of bioactive compounds such as ascorbic acid, anthocyanins and sulforaphane. Sulforaphane content increased 4 or 6 times during the first minute of microwaving in the two vegetables. [less ▲]

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See detailPolyphenol-Rich Blackcurrant Juice Prevents Endothelial Dysfunction in the Mesenteric Artery of Cirrhotic Rats with Portal Hypertension: Role of Oxidative Stress and the Angiotensin System.
Rashid, Sherzad; Idris-Khodja, Noureddine; Auger, Cyril et al

in Journal of Medicinal Food (2018), 21(4), 390-399

Chronic liver diseases with portal hypertension are characterized by a progressive vasodilatation, endothelial dysfunction, and NADPH oxidase-derived vascular oxidative stress, which have been suggested ... [more ▼]

Chronic liver diseases with portal hypertension are characterized by a progressive vasodilatation, endothelial dysfunction, and NADPH oxidase-derived vascular oxidative stress, which have been suggested to involve the angiotensin system. This study evaluated the possibility that oral intake of polyphenol-rich blackcurrant juice (PRBJ), a rich natural source of antioxidants, prevents endothelial dysfunction in a rat model of cirrhosis induced by chronic bile duct ligation (CBDL), and, if so, determined the underlying mechanism. Male Wistar rats received either control drinking water or water containing 60 mg/kg gallic acid equivalents of PRBJ for 3 weeks before undergoing surgery with CBDL or sham surgery. After 4 weeks, vascular reactivity was assessed in mesenteric artery rings using organ chambers. Both the acetylcholine-induced nitric oxide (NO)- and endothelium-dependent hyperpolarization (EDH)-mediated relaxations in mesenteric artery rings were significantly reduced in CBDL rats compared to sham rats. An increased level of oxidative stress and expression of NADPH oxidase subunits, COX-2, NOS, and of the vascular angiotensin system are observed in arterial sections in the CBDL group. Chronic intake of PRBJ prevented the CBDL-induced impaired EDH-mediated relaxation, oxidative stress, and expression of the different target proteins in the arterial wall. In addition, PRBJ prevented the CBDL-induced increase in the plasma level of proinflammatory cytokines (interleukin [IL]-1alpha, monocyte chemotactic protein 1, and tumor necrosis factor alpha) and the decrease of the anti-inflammatory cytokine, IL-4. Altogether, these observations indicate that regular ingestion of PRBJ prevents the CBDL-induced endothelial dysfunction in the mesenteric artery most likely by normalizing the level of vascular oxidative stress and the angiotensin system. [less ▲]

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See detailThe potency of commercial blackcurrant juices to induce relaxations in porcine coronary artery rings is not correlated to their antioxidant capacity but to their anthocyanin content
Tabart, Jessica ULiege; Auger, Cyril; Kevers, Claire ULiege et al

in Nutrition (2018), 51-52

Objective: Polyphenol-rich products such as fruit juices have been shown to have strong antioxidant capacities and to induce potent endothelium-dependent relaxations. We have evaluated whether the ... [more ▼]

Objective: Polyphenol-rich products such as fruit juices have been shown to have strong antioxidant capacities and to induce potent endothelium-dependent relaxations. We have evaluated whether the commercial blackcurrant juices induced endothelium-dependent relaxation of isolated coronary arteries can be related to their antioxidant capacity and/or phenolic content. Research Methods & Procedures: Six different commercial blackcurrant juices were selected. Their main phenolic compounds were measured by UPLC and antioxidant capacity was evaluated by spectrometric methods. Vascular reactivity studies with these juices were done using isolated porcine coronary arteries. Results: The six different commercial blackcurrant juices induced relaxations ranging from 21 to 100 % at the concentration of 0.5% v/v. The relaxation induced at 0.5 % v/v was not correlated to their antioxidant capacity measured by either ORAC or DPPH assays, and also not to the ascorbic acid, total polyphenols, total flavonols and total phenolic acid contents. In contrast, the amplitude of the relaxation was correlated to the total anthocyanins content and the individual anthocyanin concentration. Conclusions: Correlations between relaxation amplitude and total anthocyanin or individual anthocyanin contents are of interest for the development of functional blackcurrant beverages with the potential to promote vascular protection. [less ▲]

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See detailValidation d’une méthode UHPLC/MS-MS pour le dosage plasmatique du 15-F2t-isoprostane, biomarqueur du stress oxydant, en vue de l’établissement de valeurs de référence en routine clinique
Dubrowski, Thomas ULiege; PINCEMAIL, Joël ULiege; Vigor, Claire et al

Poster (2017, October)

Objectif : Cela fait des décennies que l’implication des isoprostanes dans le stress oxydant est mise en évidence dans la littérature. Malgré cela, il y a peu de concordances entre les différentes valeurs ... [more ▼]

Objectif : Cela fait des décennies que l’implication des isoprostanes dans le stress oxydant est mise en évidence dans la littérature. Malgré cela, il y a peu de concordances entre les différentes valeurs de référence publiées dans ces mêmes articles. Cela peut s’expliquer par le très grand nombre de composés apparentés à cette famille de molécules. L’Unité GLP-AEPT s’est concentrée sur le 15-F2t-isoprostane, biomarqueur « gold standard » de la peroxydation lipidique, afin de développer et valider une méthode de dosage fiable et utilisable en routine clinique. Cette méthode a ensuite été appliquée à l’analyse du plasma prélevé chez 61 volontaires sains en vue de déterminer les valeurs de référence pour ce biomarqueur du stress oxydant. Méthodes : Les échantillons plasmatiques sont purifiés par extraction liquide-solide avant d’être analysés par chromatographie liquide à ultra haute pression couplée à la spectrométrie de masse en tandem (UHPLC/MS-MS) au moyen du système UPLC Xevo TQ-S (Waters). Résultats : La méthode a été validée avec succès sur une gamme de concentrations allant de 25 à 2000 pg/ml en appliquant une stratégie basée sur l’erreur totale de mesure (incluant l’expression de la justesse et de la fidélité intermédiaire) et les profils d’exactitude. Les limites d’acceptation ont été fixées à ± 20 % pour les concentrations inférieures à 30 pg/ml et ± 15 % pour les concentrations égales ou supérieures à cette valeur. Cette approche garantit que seules 10 % des futures mesures d’échantillons inconnus seront en dehors de ces limites. Les concentrations obtenues pour les 61 volontaires sains sont distribuées selon une courbe gaussienne classique. L’intervalle de valeurs de référence pour le 15-F2t-isoprostane a été calculé et est compris entre 152 et 368 pg/ml [1]. Plusieurs corrélations ont ensuite pu être mises en évidence entre ce biomarqueur et d’autres paramètres biologiques connus pour affecter le niveau de stress oxydant de l’organisme (rapport Cu/Zn et glutathion total entre autres) [1]. Conclusion : Une méthode d’analyse fiable et compatible avec une application en routine clinique est à présent disponible afin de mesurer la concentration plasmatique en 15-F2t-isoprostane. Grâce à l’intervalle de valeurs de référence qui a été déterminé pour ce biomarqueur, le niveau de stress oxydant du patient peut être évalué. Références : 1. Pincemail J. et coll. Validated routine-ready UHPLC/MS-MS method for the reference range determination in human plasma of 15-F2t-isoprostane, biomarker of the oxidative stress. FRBM. 2017; 108 (1): page S42. [less ▲]

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See detailPolyphenol content and antioxidant capacity in various commercial juices
Matute Matute, Alexis Fernando ULiege; Tabart, Jessica; CHERAMY-BIEN, Jean-Paul ULiege et al

Poster (2016, November 16)

Antioxidants are enzymes, non-enzymatic proteins, minerals micronutrients, vitamins, phenolic compounds, pigments and others. Polyphenols have the ability to reduce or prevent damage caused by free ... [more ▼]

Antioxidants are enzymes, non-enzymatic proteins, minerals micronutrients, vitamins, phenolic compounds, pigments and others. Polyphenols have the ability to reduce or prevent damage caused by free radicals promoting positive effects on human health due to their antioxidant capacity. In this study we evaluated the content of polyphenols from several commercial biological (n = 6) and conventional juices (n = 6 ) (orange, blackcurrant, apple, grapes, tomato, carrot, pomegranate, lemon, grapefruit juices) and we determined the antioxidant effect by inhibition of the superoxide anion production measured by chemiluminescence technology. Conventional juices (orange, apple, tomato, pomegranate, lemon, grapefruit juices) showed more total polyphenolics (+ 32%), more flavan-3-ols (+ 67%) and more flavonols (+ 42%) than the same biological juices. The presence of anthocyanins was reported in juices made with blackcurrant, pomegranate and grapes only, with values above 50 µg/mL, regardless of their nature. Surprisingly, chemiluminescence results showed an inhibition in superoxide anion production by 51% for biological juices vs 23% in conventional ones. The correlation between total polyphenol content and antioxidant capacity of all the juices was r = 0.83 (r2 = 0.7). The fact that there is less polyphenols and higher antioxidant activity in bio juices could be related to the concentration and the type of polyphenol present. [less ▲]

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See detailModulation du stress oxydant : nouveaux concepts pour de nouvelles applications
PINCEMAIL, Joël ULiege

Scientific conference (2016, May 31)

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See detailEfficacy of the RemoweLL cardiotomy reservoir for fat and leucocyte removal from shed mediastinal blood: a randomized controlled trial.
LAGNY, Marc-Gilbert ULiege; GOTHOT, André ULiege; HANS, Grégory ULiege et al

in Perfusion (2016)

Efficacy of the RemoweLL cardiotomy reservoir for Fat and Leucocyte removal from shed mediastinal blood: a randomized controlled trial. ABSTRACT (176 words) Introduction Re-transfusion of lipid particles ... [more ▼]

Efficacy of the RemoweLL cardiotomy reservoir for Fat and Leucocyte removal from shed mediastinal blood: a randomized controlled trial. ABSTRACT (176 words) Introduction Re-transfusion of lipid particles and activated leucocytes with shed mediastinal blood (SMB) can aggravate cardiopulmonary bypass-associated inflammation and increase the embolic load. This study evaluated the fat and leucocyte removal capacity of the RemoweLL cardiotomy reservoir. Methods Forty-five patients undergoing elective on-pump cardiac surgery were randomly allocated to filtration of SMB using the RemoweLL or the Admiral cardiotomy reservoir. The primary outcome was the drop in leucocytes and lipid particles obtained with the two filters. The effect of the filters on other blood cells and inflammatory mediators such as myeloperoxidase (MPO) was also assessed. Results The RemoweLL cardiotomy filter removed 16.5 % of the leucocytes (P < 0.001) while no significant removal of leucocytes was observed with the Admiral (P = 0.48). The percentage reductions in lipid particles were similar in the two groups (26% vs 23 %, P = 0.2). Both filters similarly affected the level of MPO (P = 0.71). Discussion The RemoweLL filter more effectively removed leucocytes from SMB than the Admiral. It offered no advantage in term of lipid particle clearance. [less ▲]

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See detailEffect of Ribes nigrum leaf extracts on endothelium-dependent vasorelaxation
Tabart, Jessica; Shini-Kerth, Valérie; PINCEMAIL, Joël ULiege et al

Poster (2016, April 22)

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See detailHigh anthocyanins content of commercial blackcurrant juices induced endothelium-dependent relaxation in isolated porcine coronary arteries
Kevers, Claire ULiege; Shini-Kerth, Valérie; Tabart, Jessica et al

Poster (2016, April 22)

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See detailThe leaf extract of Ribes nigrum L. is a potent stimulator of the endothelial formation of NO in cultured endothelial cells and porcine coronary artery rings
Tabart, Jessica; Schini-Kerth, Valérie; PINCEMAIL, Joël ULiege et al

in Journal of Berry Research (2016), 6

BACKGROUND: Endothelial dysfunction is a major hallmark of most types of cardiovascular diseases. Numerous plant extracts have been shown to cause endothelium-dependent relaxations by increasing the ... [more ▼]

BACKGROUND: Endothelial dysfunction is a major hallmark of most types of cardiovascular diseases. Numerous plant extracts have been shown to cause endothelium-dependent relaxations by increasing the endothelial formation of the potent vasoprotective factor, nitric oxide (NO). OBJECTIVE: The ability of different Ribes nigrum L. extracts (Grossulariaceae) to induce endothelium-dependent relaxation by stimulating the endothelial formation of NO was assesssed. METHODS: Ribes nigrum extracts were prepared from buds, berries and leaves by extraction (Acetone:H2O:Acetic Acid; 70/28/2 (v/v/v)) and lyophilized after acetone evaporation. The ability of the extracts to stimulate the endothelial formation of NO was assessed using cultured endothelial cells and isolated porcine coronary artery rings. RESULTS: The Ribes nigrum leaf extract increased to a greater extent than the bud and the berry extracts the formation of NO, and up-regulated eNOS mRNA expression in cultured endothelial cells (the stimulatory effects amounted to 197 ± 9 %, 134 ± 6 % and 118 ± 5 %, respectively). The leaf extract induced greater relaxations of isolated coronary arteries with endothelium than the bud and the berry extracts whereas no such effects were observed in rings without endothelium. Relaxations to the leaf extract were minimally affected by indomethacin and by inhibitors of endothelium-dependent hyperpolarization response, and markedly reduced by NG-nitro-L-arginine. CONCLUSIONS: The present findings indicate that the Ribes nigrum leaf extract is a more potent inducer of the endothelial formation of NO than the bud and the berry extracts. [less ▲]

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See detailDetermination of total phenolic compounds content and antioxidant activity in cherry species and cultivars
Pissard, Audrey; Lateur, Marc; Baeten, V et al

in Journal of Berry Research (2016)

BACKGROUND: Several studies have shown that cherries, especially sour cultivars, contain substantial amounts of phenolic compounds. OBJECTIVE: This study aims to (i) analyze the total phenolic compound ... [more ▼]

BACKGROUND: Several studies have shown that cherries, especially sour cultivars, contain substantial amounts of phenolic compounds. OBJECTIVE: This study aims to (i) analyze the total phenolic compound (TPC) content and the antioxidant capacity (AC) of a large range of cultivars using the same methodology in one laboratory, and (ii) determine the possible relationship between agronomic characteristics and AC. METHODS: A total of 245 samples including sweet, sour and hybrid cultivars from our collections were harvested at their optimum maturity and characterized according to their TPC, DPPH and ORAC values. RESULTS: The TPC content and DPPH and ORAC values varied greatly among the cherries, with the sour cultivars presenting higher levels than the sweet ones. The PCA plot showed a slight grouping by species and confirmed the high TPC content level in sour cultivars. The bi-colored cultivars had lower TPC and antioxidant capacity (AC) values than dark-colored ones, indicating that coloration could give an indication of the AC of fruits. No significant relationship between the agronomic and chemical properties was highlighted. CONCLUSIONS: Cherry fruits, especially from sour cultivars, represent an important source of bioactive compounds and could attract new interest as a ‘functional food’. [less ▲]

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