References of "Munaut, Carine"
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See detailA paracrine interaction between granulosa cells and leukocytes in the preovulatory follicle causes the increase in follicular G-CSF levels
NOEL, Laure ULiege; Fransolet, Maïté ULiege; Jacobs, Nathalie ULiege et al

in J Assist Reprod Genet (2020)

OBJECTIVE: Follicular granulocyte colony-stimulating factor (G-CSF) is a new biomarker of oocyte quality and embryo implantation in in vitro fertilization (IVF) cycles. Its role in reproduction is poorly ... [more ▼]

OBJECTIVE: Follicular granulocyte colony-stimulating factor (G-CSF) is a new biomarker of oocyte quality and embryo implantation in in vitro fertilization (IVF) cycles. Its role in reproduction is poorly understood. Our study aimed to investigate the mechanisms and cells responsible for G-CSF production in the preovulatory follicle. DESIGN: Laboratory research study. SETTING: Single-center study. INTERVENTIONS: Granulosa cells and leukocytes were isolated from the follicular fluids (FF) or the blood of women undergoing IVF and from the blood of a control group of women with spontaneous ovulatory cycles to perform cocultures. MAIN OUTCOME MEASURE: G-CSF-secreted protein was quantified in the conditioned media of cocultures. RESULTS: G-CSF secretion was considerably increased in cocultures of granulosa cells and leukocytes. This effect was maximal when leukocytes were isolated from the blood of women in the late follicular phase of the menstrual cycle or from the FF of women undergoing IVF. The leukocyte population isolated from the FF samples of women undergoing IVF had a higher proportion of granulocytes than that isolated from the corresponding blood samples. Leukocytes induced the synthesis and secretion of G-CSF by granulosa cells. Among a range of other FF cytokines/chemokines, only growth-regulated oncogene alpha (GROalpha) was also increased. CONCLUSION: The notable rise in G-CSF at the time of ovulation coincides with the accumulation of follicular granulocytes, which stimulate G-CSF production by granulosa cells via paracrine interactions. High follicular G-CSF concentrations may occur in follicles with optimal granulosa-leukocyte interactions, which could explain the increased implantation rate of embryos arising from these follicles. [less ▲]

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See detailSlow Freezing Versus Vitrification of Mouse Ovaries: from Ex Vivo Analyses to Successful Pregnancies after Auto-Transplantation
Terren, Carmen ULiege; Fransolet, Maïté ULiege; Ancion, Marie ULiege et al

in Scientific Reports (2019), 9(1), 19668

Slow freezing (SF) is the reference method for ovarian tissue cryopreservation. Vitrification (VT) constitutes an alternative but controversial method. This study compares SF and VT (open [VTo] and closed ... [more ▼]

Slow freezing (SF) is the reference method for ovarian tissue cryopreservation. Vitrification (VT) constitutes an alternative but controversial method. This study compares SF and VT (open [VTo] and closed [VTc] systems) in terms of freezing damage and fertility restoration ability. In vitro analyses of C57Bl/6 SF or VTo-ovaries, immediately after thawing/warming or after culture (cult), revealed that event though follicular density was similar between all groups, nuclear density was decreased in VTo-ovaries compared to CT-ovaries (CT = 0.50 +/- 0.012, SF = 0.41 +/- 0.03 and VTo = 0.29 +/- 0.044, p < 0.01). Apoptosis was higher in VTo-cult ovaries compared to SF-cult ovaries (p < 0.001) whereas follicular Bmp15 and Amh gene expression levels were decreased in the ovaries after culture, mostly after VTo (p < 0.001). Natural mating after auto-transplantation of SF, VTo and VTc-ovaries revealed that most mice recovered their oestrous cycle. Fertility was only restored with SF and VTo ovaries (SF: 68%; VTo: 63%; VTc: 0%; p < 0.001). Mice auto-transplanted with SF and VTo-ovaries achieved the highest number of pregnancies. In conclusion, in vitro, no differences between SF and VTo were evident immediately after thawing/warming but VTo ovaries displayed alterations in apoptosis and follicular specific proteins after culture. In vivo, SF and VTo ovary auto-transplantation fully restored fertility whereas with VTc-ovary auto-transplantation no pregnancies were achieved. [less ▲]

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See detailAbsence of correlation between follicular fluid volume and follicular granulocyte colony-stimulating factor, a predictor of embryo implantation and successful delivery.
NOEL, Laure ULiege; Donneau, Anne-Françoise ULiege; JOUAN, Caroline ULiege et al

in Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology (2019)

Follicular granulocyte colony-stimulating factor (G-CSF) is a documented marker of embryo implantation potential. The primary objective was to determine whether follicular G-CSF levels correlate with ... [more ▼]

Follicular granulocyte colony-stimulating factor (G-CSF) is a documented marker of embryo implantation potential. The primary objective was to determine whether follicular G-CSF levels correlate with follicular fluid volume. The secondary objectives were to assess whether follicular G-CSF is associated with oocyte maturity at the time of harvest and with delivery rate after fresh or frozen embryo transfer. Thirty-two patients undergoing intracytoplasmic sperm injection (ICSI) cycles were recruited (Centre de Procreation Medicalement Assistee (CPMA), University of Liege, Belgium). A total of 211 follicular fluid (FF) samples were individually collected at the time of oocyte harvest. FF volume was recorded, and G-CSF concentration was assessed by ELISA. The embryos were individually cultured in vitro. Their implantation and live birth rates were recorded after fresh and frozen embryo transfers. The follicular fluid volume did not correlate with the follicular G-CSF concentration. There were no differences in follicular G-CSF levels between mature and immature oocytes. The probability of successful implantation and delivery was increased for embryos with FF containing a high G-CSF concentration. There was a trend toward lower follicular G-CSF levels in cases of miscarriage. Therefore, follicular fluid volume cannot be a substitute for follicular G-CSF as a marker of embryo implantation ability. [less ▲]

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See detailClinical performance of a specific granulocyte colony stimulating factor ELISA to determine its concentration in follicular fluid as a predictor of implantation success during in vitro fertilization.
Tournaye, H; D'Hooghe, T.; Verheyen, G. et al

in Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology (2019)

This study aimed to demonstrate the clinical performance of an ultra-sensitive follicular fluid (FF) granulocyte colony stimulating factor (G-CSF) immunoassay to confirm previous work, indicating a ... [more ▼]

This study aimed to demonstrate the clinical performance of an ultra-sensitive follicular fluid (FF) granulocyte colony stimulating factor (G-CSF) immunoassay to confirm previous work, indicating a correlation between FF G-CSF concentration and live birth potential of the corresponding embryo after in vitro fertilization. This study was a noninterventional, prospective, diagnostic clinical multicentric study conducted between August 2012 and January 2014 with 396 single embryo transfers (SETs) from 278 subjects. During oocyte retrieval, FF was individually collected. Embryo morphology and implantation success were evaluated. The implantation success rate in the high G-CSF group (32.3%) was higher than the overall rate (27.5%). Similarly, for embryos with optimal morphology, implantation success rates were highest among those in the high G-CSF concentration category (34.5%) compared with low (19.6%) and intermediate (29.8%) G-CSF concentration categories. Significant differences in mean G-CSF concentrations were observed between the study sites. To minimize bias, analyses were repeated using data from the center with the largest number of SETs. In alignment with the overall analysis, this center demonstrated a 43% greater probability of implantation for optimal embryos with high G-CSF compared to the general implantation rate among optimal embryos and a 327% increase compared with the implantation rate of optimal embryos with low G-CSF. [less ▲]

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See detailEvaluation of Z-VAD-FMK as an anti-apoptotic drug to prevent granulosa cell apoptosis and follicular death after human ovarian tissue transplantation
Fransolet, Maïté ULiege; NOEL, Laure ULiege; HENRY, Laurie ULiege et al

in Journal of Assisted Reproduction and Genetics (2019)

Purpose To evaluate the efficiency of ovarian tissue treatment with Z-VAD-FMK, a broad-spectrum caspase inhibitor, to prevent follicle loss induced by ischemia/reperfusion injury after transplantation ... [more ▼]

Purpose To evaluate the efficiency of ovarian tissue treatment with Z-VAD-FMK, a broad-spectrum caspase inhibitor, to prevent follicle loss induced by ischemia/reperfusion injury after transplantation. Methods In vitro, granulosa cells were exposed to hypoxic conditions, reproducing early ischemia after ovarian tissue trans- plantation, and treated with Z-VAD-FMK (50 μM). In vivo, cryopreserved human ovarian fragments (n = 39) were embedded in a collagen matrix containing or not Z-VAD-FMK (50 μM) and xenotransplanted on SCID mice ovaries for 3 days or 3 weeks. Results In vitro, Z-VAD-FMK maintained the metabolic activity of granulosa cells, reduced HGL5 cell death, and decreased PARP cleavage. In vivo, no improvement of follicular pool and global tissue preservation was observed with Z-VAD-FMK in ovarian tissue recovered 3-days post-grafting. Conversely, after 3 weeks of transplantation, the primary follicular density was higher in fragments treated with Z-VAD-FMK. This improvement was associated with a decreased percentage of apoptosis in the tissue. Conclusions In situ administration of Z-VAD-FMK slightly improves primary follicular preservation and reduces global apo- ptosis after 3 weeks of transplantation. Data presented herein will help to guide further researches towards a combined approach targeting multiple cell death pathways, angiogenesis stimulation, and follicular recruitment inhibition. [less ▲]

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See detailEndometriosis: a deep insight into the pathology through metabolomics
Leenders, Justine ULiege; Martin, Manon; NISOLLE, Michelle ULiege et al

Poster (2018, June)

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See detailEndometriosis: deep insight into the pathology through metabolomics
Leenders, Justine ULiege; Martin, Manon; NISOLLE, Michelle ULiege et al

Poster (2018, May)

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See detailHeterogeneity of estrogen receptor alpha and progesterone receptor distribution in lesions of deep infiltrating endometriosis of untreated women or during exposure to various hormonal treatments.
BRICHANT, Géraldine ULiege; NERVO, Patricia ULiege; Albert, Adelin ULiege et al

in Gynecological Endocrinology (2018)

Deep infiltrating endometriosis (DIE) responds variably to hormonal therapy. Mutations in cancer driver genes have been identified in a fraction of the ectopic endometrial epithelial cells, suggesting a ... [more ▼]

Deep infiltrating endometriosis (DIE) responds variably to hormonal therapy. Mutations in cancer driver genes have been identified in a fraction of the ectopic endometrial epithelial cells, suggesting a functional heterogeneity of these lesions. To evaluate the phenotype heterogeneity of cells in DIE, we measured the expression of estrogen receptor alpha (ERalpha) and of progesterone receptor (PR) in DIE of untreated women or under various treatments. We analyzed the luminal epithelial height (LEH), immunoreactive epithelial staining (IRS) and stromal staining intensity (SSI) of ERalpha and PR. We observed a high variability in the same gland, among distinct glands in the same sample and among distinct patients receiving the same treatment. LEH variability was primarily due to epithelial cells heterogeneity in a gland, secondarily to the glands randomly evaluated on the same section, and tertiary to the patient category. Variability in IRS and SSI scores was primarily the consequence of their heterogeneity in the same woman and to a lesser extent to variability among patients. LEH and SSI were not modified according to treatment. IRS for PR was lower in treated patients. This heterogeneity of ERalpha and PR distribution could explain why endocrine treatments are unable to cure this condition. [less ▲]

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See detailAccumulation of IL-17+ Vgamma6+ gamma-delta T cells in pregnant mice is not associated with spontaneous abortion
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Perrier d'Hauterive, Sophie ULiege et al

in Clinical and Translational Immunology (2018), 7

Introduction. Pregnancy is an immune paradox. While the immune system is required for embryo implantation, placental development and progression of gestation, excessive inflammation is associated with ... [more ▼]

Introduction. Pregnancy is an immune paradox. While the immune system is required for embryo implantation, placental development and progression of gestation, excessive inflammation is associated with pregnancy failure. Similarly, the cytokine IL-17A plays an important role in defence against extracellular pathogens, but its dysregulation can lead to pathogenic inflammation and tissue damage. Although expression of IL-17 has been reported during pregnancy, the cellular source of this cytokine and its relevance to gestation are not clear. Objectives. Here we define the kinetics and cellular source of IL-17A in the uterus during healthy and abortion-prone murine pregnancy. Methods. The CBA/J x DBA/2J abortion-prone mating was used and compared to CBA/J x BALB/c control mating. Results. We demonstrate that, irrespective of gestational health, the number of IL-17-producing cells peaks during midterm pregnancy and is largely derived from the gd T-cell lineage. We identify cd T, Th17, CD8 T and NKT cells as the cellular source of IL-17A in pregnant mice. Furthermore, we positively identify the Vc6+ subset of uterine gd T cells as the main producer of IL-17A during both healthy pregnancy and abortive pregnancy. Conclusions. To conclude, the accumulation of uterine IL-17+ innate-like T cells appears not to adversely impact the developing foetus. Collectively, our results show that IL-17+ gd T cells are present in the uterus throughout the course of normal gestation and therefore may play an important role in healthy pregnancy. Keywords: abortion, gamma delta T cells, interleukin-17, pregnancy. [less ▲]

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See detailESTROGEN RECEPTORS AND ESTETROL-DEPENDENT NEUROPROTECTIVE ACTIONS: A PILOT STUDY
Tskitishvili, Ekaterine ULiege; Pequeux, Christel ULiege; Munaut, Carine ULiege et al

Conference (2017, October 20)

Context: Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic–ischemic encephalopathy. Objective: We aimed to define ... [more ▼]

Context: Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic–ischemic encephalopathy. Objective: We aimed to define the role of estrogen receptors in E4-dependent actions in neuronal cell cultures and prove the promyelinating effect of E4. Methods: In vitro the antioxidative and cell survival/proliferating effects of E4 on H2O2-induced oxidative stress in primary hippocampal cell cultures were studied using different combinations of specific inhibitors for ERα (MPP dihydrochloride), ERβ (PHTTP), GPR30 (G15) and palmytoilation (2-BR). LDH activity and cell survival assays were performed. In vivo the promyelinating role of different concentrations of E4 (1 mg/kg/day, 5 mg/kg/day, 10 mg/kg/day, 50 mg/kg/day) was investigated using the hypoxic–ischemic brain damage model in the 7-day-old immature rats before/after the induction of hypoxic–ischemic insult. Myelin basic protein (MBP) immunostaining was performed on brain coronal sections. Results: Our results show that LDH activity is significantly upregulated in cell cultures where the E4’s effect was completely blocked by concomitant treatment either with ERα and ERβ inhibitors (MPP and PHTPP, respectively), or ERα and ERβ inhibitors combined with 2-BR. Cell survival is significantly downregulated in cell cultures where the effect of E4 was blocked by ERβ inhibitor (PHTTP) alone. The blockage of GRP30 receptor did affect neither LDH activity nor cell survival. MBP immunostaining is significantly upregulated in E4-pretreated groups at a concentration of 5 mg/kg/day and 50 mg/kg/day E4, whereas the MBP-positive area OD ratio is significantly increased in all the E4-treated groups. Conclusions: E4’s antioxidative actions mostly depend on ERα and ERβ, whereas neurogenesis and possibly promyelinating activities might be realized through ERβ. [less ▲]

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See detailCombining strong sparsity and competitive predictive power with the L‑sOPLS approach for biomarker discovery in metabolomics
Feraud, Baptiste; Munaut, Carine ULiege; Manon, Martin et al

in Metabolomics (2017), (13), 130

In the context of metabolomics analyses, partial least squares (PLS) represents the standard tool to perform regression and classification. OPLS, the Orthogonal extension of PLS which has proved to be ... [more ▼]

In the context of metabolomics analyses, partial least squares (PLS) represents the standard tool to perform regression and classification. OPLS, the Orthogonal extension of PLS which has proved to be very useful when interpretation is the main issue, is a more recent way to decompose the PLS solution into predictive components correlated to the target Y and components pertaining to the data X but uncorrelated to Y. This predominance of (O)PLS can raise the question of the awareness of alternative multivariate regression and/or classification tools able to find biomarkers. Actually, the search for biomarkers remains a key issue in metabolomics as it is crucial to very accurately target discriminating features. [less ▲]

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See detailMetabolomics approaches of endometriosis
Leenders, Justine ULiege; Munaut, Carine ULiege; NISOLLE, Michelle ULiege et al

Conference (2017, September 12)

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See detailImproved computer-assisted analysis of the global lymphatic network in human cervical tissues.
BALSAT, Cédric ULiege; Signolle, N; Goffin, Frédéric ULiege et al

in Modern Pathology (2017), 30(2), 313

Detailed reference viewed: 52 (10 ULiège)