References of "Mouithys-Mickalad, Ange"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailModulation of mitochondrial respiration rate and calcium-induced swelling by new cromakalim analogues.
Mouithys-Mickalad, Ange ULiege; Ceusters, Justine ULiege; Charif, Mounia et al

in Chemico-Biological Interactions (2020), 331

A cellular model of cardiomyocytes (H9c2 cell line) and mitochondria isolated from mouse liver were used to understand the drug action of BPDZ490 and BPDZ711, two benzopyran analogues of the reference ... [more ▼]

A cellular model of cardiomyocytes (H9c2 cell line) and mitochondria isolated from mouse liver were used to understand the drug action of BPDZ490 and BPDZ711, two benzopyran analogues of the reference potassium channel opener cromakalim, on mitochondrial respiratory parameters and swelling, by comparing their effects with those of the parent compound cromakalim. For these three compounds, the oxygen consumption rate (OCR) was determined by high-resolution respirometry (HRR) and their impact on adenosine triphosphate (ATP) production and calcium-induced mitochondrial swelling was investigated. Cromakalim did not modify neither the OCR of H9c2 cells and the ATP production nor the Ca-induced swelling. By contrast, the cromakalim analogue BPDZ490 (1) induced a strong increase of OCR, while the other benzopyran analogue BPDZ711 (2) caused a marked slowdown. For both compounds, 1 displayed a biphasic behavior while 2 still showed an inhibitory effect. Both compounds 1 and 2 were also found to decrease the ATP synthesis, with pronounced effect for 2, while cromakalim remained without effect. Overall, these results indicate that cromakalim, as parent molecule, does not induce per se any direct effect on mitochondrial respiratory function neither on whole cells nor on isolated mitochondria whereas both benzopyran analogues 1 and 2 display totally opposite behavior profiles, suggesting that compound 1, by increasing the maximal respiration capacity, might behave as a mild uncoupling agent and compound 2 is taken as an inhibitor of the mitochondrial electron-transfer chain. [less ▲]

Detailed reference viewed: 23 (1 ULiège)
Full Text
Peer Reviewed
See detailBlack Soldier Fly (Hermetia Illucens) Larvae Protein Derivatives: Potential to Promote Animal Health.
Mouithys-Mickalad, Ange ULiege; Schmitt, Eric; Dalim, Monika et al

in Animals (2020), 10(6),

European legislation permits the inclusion of insect proteins in pet and aquaculture diets. Black soldier fly larvae (BSF) are one of the most actively produced species due to their low environmental ... [more ▼]

European legislation permits the inclusion of insect proteins in pet and aquaculture diets. Black soldier fly larvae (BSF) are one of the most actively produced species due to their low environmental impact and nutritional characteristics. BSF protein derivatives (proteins and protein hydrolysates) contain a substantial amount of low molecular weight peptides that are known to possess antioxidant potential. In this study, the in vitro antioxidant potential of commercial BSF proteins and protein hydrolysates was investigated for (1) radical scavenging activity, (2) myeloperoxidase activity modulation, and (3) neutrophil response modulation. Chickenmeal and fishmeal are commonly used in pet food and aquaculture formulations. Hence, both were used as industrial benchmarks during this study. The results indicate that fishmeal and chickenmeal are ineffective at suppressing the oxidative damage caused by neutrophil response and myeloperoxidase activity. Fishmeal and chickenmeal even exhibit pro-oxidant behavior in some of the models used during this study. On the other hand, it was found that BSF protein derivatives could be effective in protecting against the cellular damage resulting from neutrophil and myeloperoxidase activities. The outcomes of this study indicate that BSF protein derivatives could be potentially included in pet food and aquaculture feed formulations as health-promoting ingredients. [less ▲]

Detailed reference viewed: 22 (4 ULiège)
Full Text
Peer Reviewed
See detailIn vitro Antileishmanial, Antitrypanosomal, and Anti-inflammatory- like Activity of Terminalia mollis Root Bark
Muganga, Raymond; Bero, Joanne; Quetin-Leclercq, Joelle et al

in Planta Medica (2020), efirst

This study aims at determining the in vitro antitrypanosomal, antileishmanial, antioxidant, and anti-inflammatory-like activities of Terminalia mollis root crude extracts. The antitrypanosomal and ... [more ▼]

This study aims at determining the in vitro antitrypanosomal, antileishmanial, antioxidant, and anti-inflammatory-like activities of Terminalia mollis root crude extracts. The antitrypanosomal and antileishmanial activities on Trypanosoma brucei brucei (strain 427) and promastigotes of Leishmania mexicana mexicana (MHOM/BZ/84/BEL46) were evaluated in vitro. The methanolic root bark extract and standards were profiled by HPLC-PDA, and the majority of compounds identified using literature data. The in vitro antioxidant and anti-inflammatory-like activities were determined by evaluating the effect of crude extracts on reactive oxygen species produced by phorbol 12-myristate 13-acetate-stimulated equine neutrophils using lucigenin-enhanced chemiluminescence and on purified equine myeloperoxidase activity measured by specific immunological extraction followed by enzymatic detection. The methanolic, aqueous crude extract, and aqueous crude extract free of tannins exhibited good growth inhibition on Trypanosoma brucei brucei (IC50 3.72, 6.05, and 4.45 µg/mL respectively) but were inactive against Leishmania mexicana mexicana (IC50 > 100 µg/mL). Suramin (IC50 0.11 µg/mL) and amphotericin (IC50 0.11 µg/mL) were used as standard respectively for the antitrypanosomal and antileishmanial activity. Very interesting antioxidant and anti-inflammatory-like activities were observed with 50% hydroethanolic, aqueous crude extracts, and aqueous crude extract free of tannins as well as with pure punicalagin, gallic, and ellagic acid (IC50 0.38 – 10.51 µg/mL for 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), chemiluminescence, and specific immunological extraction followed by enzymatic detection assays. The study results support traditional medicinal use of the plant for the treatment of parasitical disorders and revealed for the first time the antitrypanosomal potential, anti-inflammatory-like, and antioxidant activity of Terminalia mollis root. [less ▲]

Detailed reference viewed: 43 (12 ULiège)
Full Text
See detailDetection of mitochondrial dysfunction by blood mononuclear cells analysis
Kruse, Caroline ULiege; Art, Tatiana ULiege; Mouithys-Mickalad, Ange ULiege et al

Poster (2019, November 21)

Since mitochondria play diverse crucial roles within the cell, mitochondrial dysfunction can have disastrous consequences. High resolution respirometry (HRR) is a new tool that allows the quantification ... [more ▼]

Since mitochondria play diverse crucial roles within the cell, mitochondrial dysfunction can have disastrous consequences. High resolution respirometry (HRR) is a new tool that allows the quantification of mitochondrial oxidative phosphorylation and electron transfer capacities. This tool allows an analysis of mitochondrial bioenergetics and the main substrate pathway of the cells that are of interest. In human, platelets and peripheral blood mononuclear cells have been used as biomarkers in diverse pathological models, as diabetes, atherosclerosis, cancer and neurodegenerative diseases. In the horse, HRR studies have been conducted on skeletal muscles samples. Using this technique, a so-called “mitochondrial syndrome” has been reported in equine atypical myopathy, an environmental intoxication, which is characterized by a severe decrease of mitochondrial respiratory capacity in affected individuals. However, the muscle biopsy procedure is difficult to use in equine clinical routine. Therefore, the aim of this study was to test whether mitochondrial function could be assessed on equine blood mononuclear cells with respirometry. Whole blood was collected in 9 ml EDTA tubes in six horses. Cells of interest (PBMC) were isolated with a density-separation medium (Lymphoprep™) following a standardized procedure. Electron paramagnetic resonance as well as HRR analysis were performed within the following 6 hours. With this isolation technique more than 1x106 PBMC/ml of whole blood were obtained. Respirometric analysis with PBMC taken from the same horses indicated a lack of reproducibility. This finding seems to be primarily due to a too long timeframe (i.e. ±Xh) between cell isolation and respirometric analysis. Additional studies indicated that when respirometry was performed within the first 2 hours after cell isolation, standard variation between samples was significantly reduced. Up to now a correlation between skeletal muscle fiber and PBMC mitochondrial bioenergetics in the equine species is not possible to be established. A standardization of the procedure is vital for reproducibility between laboratories and comparison between healthy and ill horses. The next steps are to compare the oxygraphic measures between equine PBMC and muscle biopsies, as well as the comparison of mitochondrial function between healthy and horses in extreme metabolic conditions (e.g atypical myopathy). [less ▲]

Detailed reference viewed: 51 (11 ULiège)
Full Text
See detailEffect of morphine and propofol on the activation of equine neutrophils: An EPR spin trapping study
Nyssen, Pauline ULiege; Mouithys-Mickalad, Ange ULiege; Hoebeke, Maryse ULiege

Poster (2019, May 24)

Inflammation is a complex phenomenon involving chemical and enzymatic mechanisms. The polymorphonuclear neutrophil leukocytes (PMNs) play an essential immunomodulatory role by releasing harmful reactive ... [more ▼]

Inflammation is a complex phenomenon involving chemical and enzymatic mechanisms. The polymorphonuclear neutrophil leukocytes (PMNs) play an essential immunomodulatory role by releasing harmful reactive oxygen species (ROS) and oxidant enzymes. During the respiratory burst, the enzymatic complex NADPH oxidase produces the ROS precursor: superoxide anion (O_2^( . −)), to initiate the invasive microorganisms degradation. Inflammatory pathologies induce an excessive stimulation of the neutrophils and an uncontrolled production and release of ROS in the extracellular medium, leading to severe tissue damages. The treatment of chronic pathologies is still a challenge to rise. For this reason, the research of potential anti-inflammatory molecules, able to inhibit the oxidant enzymes and/or to scavenge ROS, is an important part of biomedical research. Morphine and propofol, in addition to their analgesic and anaesthetic action respectively, presents antioxidant properties 3,4. However, only few data has been reported on their potential superoxide anion scavenger and inhibitory effect on the neutrophil respiratory burst. [less ▲]

Detailed reference viewed: 31 (5 ULiège)
Full Text
Peer Reviewed
See detailThe soluble curcumin derivative NDS27 inhibits superoxide anion production by neutrophils and acts as substrate and reversible inhibitor of myeloperoxidase
Franck, Thierry ULiege; Aldib, I.; Zouaoui Boudjeltia, K. et al

in Chemico-Biological Interactions (2019), 297

A water-soluble curcumin lysinate incorporated into hydroxypropyl-β-cyclodextrin (NDS27) has been developed and shown anti-inflammatory properties but no comparative study has been made in parallel with ... [more ▼]

A water-soluble curcumin lysinate incorporated into hydroxypropyl-β-cyclodextrin (NDS27) has been developed and shown anti-inflammatory properties but no comparative study has been made in parallel with its parent molecule, curcumin on polymorphonuclear neutrophils (PMNs) and myeloperoxidase (MPO) involved in inflammation. The effect of NDS27, its excipients (hydroxypropyl-β-cyclodextrin and lysine), curcumin lysinate and curcumin were compared on the release of superoxide anion by PMNs using a chemiluminescence assay and on the enzymatic activity of MPO. It was shown that curcumin and NDS27 exhibit similar inhibition activities on superoxide anion release by stimulated PMNs but also on MPO peroxidase and halogenation activities. The action mechanism of curcumin and NDS27 on the MPO activity was refined by stopped-flow and docking analyses. We demonstrate that both curcumin and NDS27 are reversible inhibitors of MPO by acting as excellent electron donors for redox intermediate Compound I (∼107 M−1 s−1) but not for Compound II (∼103 M−1 s−1) in the peroxidase cycle of the enzyme, thereby trapping the enzyme in the Compound II state. Docking calculations show that curcumin is able to enter the enzymatic pocket of MPO and bind to the heme cavity by π-stacking and formation of hydrogen bonds involving substituents from both aromatic rings. Hydroxypropyl-β-cyclodextrin is too bulky to enter MPO channel leading to the binding site suggesting a full release of curcumin from the cyclodextrin thereby allowing its full access to the active site of MPO. In conclusion, the hydroxypropyl-β-cyclodextrin of NDS27 enhances curcumin solubilization without affecting its antioxidant capacity and inhibitory activity on MPO. © 2018 Elsevier B.V. [less ▲]

Detailed reference viewed: 83 (16 ULiège)
Full Text
Peer Reviewed
See detailCapillary electrophoresis, high-performance liquid chromatography, and thin-layer chromatography analyses of phenolic compounds from rapeseed plants and evaluation of their antioxidant activity.
Huang, Yang ULiege; Jansen, Olivia ULiege; Frederich, Michel ULiege et al

in Journal of Separation Science (2019)

Rapeseed plants, known for oil production, are also known to contain phenolic compounds such as phenolic acids and flavonoids, with potential antioxidant and anticancer activities. The separation and ... [more ▼]

Rapeseed plants, known for oil production, are also known to contain phenolic compounds such as phenolic acids and flavonoids, with potential antioxidant and anticancer activities. The separation and identification of 11 phenolic acids in rapeseed extracts (including leaves, flowers, Chinese seeds, Belgian seeds, and cake) by capillary electrophoresis were investigated. The results were compared with those obtained with high-performance liquid chromatography and thin-layer chromatography and showed that the capillary electrophoresis technique offers several advantages for the identification of phenolic compounds in various rapeseed extracts. The antioxidant activity of rapeseed extracts and reference compounds was evaluated using four different approaches, namely, 2,2'-azinobis- (3-ethylbenzohiazoline-6-sulfonic acid assay, free radical 2,2-diphenyl-1-picrylhydrazyl assay, electron paramagnetic resonance spectroscopy and the measurement of the total polyphenol content. The contents of total polyphenols in the tested extracts were ranging between 5.4 and 21.1% m/m and ranked as follows: Chinese seeds > Belgian seeds > Flowers > Cake > Leaves. [less ▲]

Detailed reference viewed: 40 (10 ULiège)
Full Text
See detailPeripheral blood mononuclear cells to detect mitochondrial dysfunction in horses: a preliminary study
Kruse, Caroline ULiege; Art, Tatiana ULiege; Mouithys-Mickalad, Ange ULiege et al

Conference (2019)

Mitochondrial dysfunction has emerged as a ubiquitous cause of disease in human and animals. In the equine species, muscle fibers were analyzed to detect and assess defective mitochondrial function. Using ... [more ▼]

Mitochondrial dysfunction has emerged as a ubiquitous cause of disease in human and animals. In the equine species, muscle fibers were analyzed to detect and assess defective mitochondrial function. Using respirometry, a so-called “mitochondrial syndrome” has been reported in equine atypical myopathy, which is characterized by a severe decrease of mitochondrial respiratory capacity in affected individuals [1] . Up to now, respirometric studies in horses have been successfully conducted on skeletal muscles samples. However, the muscle biopsy procedure is rather invasive and therefore difficult to use in equine clinical routine. Despite the fact that peripheral blood mononuclear cells (PBMC) have been used in human medicine to assess mitochondrial function, there is only one study comparing PBMC and skeletal muscle oxidative capacity in monkeys so far [2]. Therefore, the aim of this study was to test whether mitochondrial function can be assessed on equine blood cells with respirometry, according to the fact that blood is a biological sample easier to collect than muscle. Whole blood was collected in 9 ml EDTA tubes in four horses. Cells of interest (PBMC) were isolated with a density-separation medium (Lymphoprep™) within one hour of sampling, following a standardized procedure. Electron paramagnetic resonance as well as the respirometric analysis were performed within the following 4 hours. The preliminary tests show that recorded respirations are similar to those documented in humans. However, a lack of reproducibility of respirometric analysis with PBMC taken from the same horses was observed. Therefore, a correlation between skeletal muscle fiber and PBMC mitochondrial bioenergetics in the equine species is, up to now, not possible to be established. Nevertheless, an efficient PBMC isolation protocol was established and mitochondrial respiration with respirometry was obtained. The cause of the variations within the same individual is still under examination. [less ▲]

Detailed reference viewed: 59 (7 ULiège)
Full Text
Peer Reviewed
See detailAnti-inflammatory and antioxidant activities of Rungia congoensis, a traditional vegetable consumed by Yombe people from Kongo Central area (DR. Congo).
Mutwale Kapepula, Paulin; Baku Ngumbi, Pacha; Kasongo Kawayidiko, Michel et al

in Natural Product Research (2019)

Rungia congoensis, a traditional vegetable from Kongo Central area (DR. Congo) was studied for establishing microscopic characters and characterised by chromatographic techniques and their in vitro ... [more ▼]

Rungia congoensis, a traditional vegetable from Kongo Central area (DR. Congo) was studied for establishing microscopic characters and characterised by chromatographic techniques and their in vitro biochemical activities against ROS production were evaluated in cellular models and on an enzyme, myeloperoxidase (MPO), involved in inflammation. Microscopically leaf can be characterised by non-glandular and glandular trichomes, sinuous anticlinal epidermal cells, diacytic stomata and helical vessels. Methanolic extract displayed high cellular antioxidant activity at the concentrations range of 0.1-10 mug mL(-1) and 1-20 mug mL(-1) using lucigenin on neutrophils and DCFH-DA on HL 60, respectively. This extract also showed, more efficient effects on extracellular and intracellular ROS production and MPO activity. Antioxidant and anti-inflammatory activities of R. congoensis were significantly higher, positively correlated with their phytochemical constituents such as flavonoids, iridoids and phenolic acids; and could justify their use as traditional vegetable and potent local nutraceutical resource. [less ▲]

Detailed reference viewed: 35 (10 ULiège)
Full Text
See detailIn vitro testing of equine atypical myopathy induced mitochondrial dysfunction
Zhou, Y; Gustin, Pascal ULiege; Mouithys-Mickalad, Ange ULiege et al

Poster (2018, October 26)

Detailed reference viewed: 23 (6 ULiège)
Full Text
Peer Reviewed
See detailMorphine, a potential inhibitor of myeloperoxidase activity
Nyssen, Pauline ULiege; Mouithys-Mickalad, Ange ULiege; MINGUET, Grégory ULiege et al

in Biochimica et Biophysica Acta - General Subjects (2018), 1862(10), 2236-2244

Morphine is an opioid alkaloid commonly used in clinical practice for its analgesic properties. The phenolic hydroxyl group of that phenanthrene derivative is pivotal for binding to opioid receptors but ... [more ▼]

Morphine is an opioid alkaloid commonly used in clinical practice for its analgesic properties. The phenolic hydroxyl group of that phenanthrene derivative is pivotal for binding to opioid receptors but it may also be responsible for the antioxidant behavior of morphine reported in several in vitro experiments. In this study, we assessed the effect of morphine on myeloperoxidase (MPO), a hemic enzyme from azurophilic granules of polymorphonuclear neutrophils involved in the production of cytotoxic and microbicidal reactive oxidants during inflammatory response. Specific immunological extraction followed by enzyme detection (SIEFED) and molecular modeling (docking) were performed to study the potential anti-catalytic action of morphine on MPO in comparison with the inhibitory effects of reference antioxidant molecules quercetin, gallic acid and ascorbic acid. The reducing action of morphine on the MPO peroxidase cycle has been investigated using electron paramagnetic resonance (EPR) and UV-visible absorption spectroscopy. Morphine acted as a reducing substrate in the peroxidase cycle of MPO and therefore protected the enzyme against the suicide action of its natural substrate, hydrogen peroxide. The SIEFED experiments associated with the docking study, further demonstrated a lack of strong and sustained anti-catalytic activity of morphine. In summary, from the results of this study, it appears that morphine acts as a weak and reversible inhibitor of MPO that may nonetheless contribute to immunomodulatory and antioxidant effects of this opioid analgesic in vivo. © 2018 Elsevier B.V. [less ▲]

Detailed reference viewed: 32 (13 ULiège)
Full Text
Peer Reviewed
See detailFunctional diagnosis of mitochondrial defects in equine atypical myopathy and targeted mitochondrial therapy: a preliminary study.
Zhou, Y; Gonzalez-Medina; Gustin, Pascal ULiege et al

in In proceedings: 13th Conference on Mitochondrial Physiology (2018, September)

Detailed reference viewed: 32 (7 ULiège)
Full Text
Peer Reviewed
See detailPhytochemical studies, antioxidant activity and total phenolic content of Ferula communis L. organ extracts
Rahali, Fatma Zohra; Kefi, Sarra; Rebey, Iness Bettaieb et al

in Plant Biosystems (2018)

The present study aimed to assess antioxidant activities of three organs (flower, fruit and stem) extracts of Tunisian Ferula (F.). Various experimental models were used for the characterization of ... [more ▼]

The present study aimed to assess antioxidant activities of three organs (flower, fruit and stem) extracts of Tunisian Ferula (F.). Various experimental models were used for the characterization of antioxidant activities, in vitro and using dichlorofluorescein (DCF) induced fluorescence techniques from phorbol myristate acetate (PMA ) stimulated human myeloid cell line HL-60communis. Results showed that the antioxidant activities varied considerably with organs. Thus, flower exhibited higher DPPH• scavenging ability, reducing and chelating power than stem and fruit. Moreover antioxidant capacities using ORAC method and a cell based-assay showed that fruit and stem exhibited statistically similar antioxidant activities. Moreover, F. communis contains high amounts of flavonoids with various health benefits as antioxidant properties attributed to their antioxidant potential. Likewise, to obtain biologically relevant information, the antioxidant activities of the extracts were evaluated on cellular models implicating the antioxidant activities; this test generally showed that F. communis flower extracts have the highest antioxidant capacities correlated to the highest total phenolic content. The identification of phenolic compounds in F. communis extracts using RP-HPLC revealed that resorcinol, ferulic and syringic acids together with coumarin were the major molecules. [less ▲]

Detailed reference viewed: 153 (10 ULiège)
Full Text
Peer Reviewed
See detailPhytochemical composition and antioxidant activities of different aerial parts extracts of Ferula communis L.
Rahali, F. Z.; Kefi, S.; Bettaieb Rebey, I. et al

in Plant Biosystems (2018)

The present study aimed to assess antioxidant activities of three organs (flower, fruit, and stem) extracts of Tunisian Ferula (F.) communis. Various experimental models were used to characterize the ... [more ▼]

The present study aimed to assess antioxidant activities of three organs (flower, fruit, and stem) extracts of Tunisian Ferula (F.) communis. Various experimental models were used to characterize the antioxidant activities in vitro as well as on ROS-induced fluorescence using dichlorofluorescein technique from phorbol myristate acetate (PMA)-stimulated human myeloid cell line HL-60. Results showed that the antioxidant activities varied considerably with organs. Thus, flower exhibited higher DPPH-scavenging ability, reducing and chelating power than stem and fruit. Also, antioxidant capacities using ORAC method and a cell-based assay showed that fruit and stem exhibited statistically similar antioxidant activities. Moreover, F. communis contains high amounts of flavonoids with various health benefits attributed to their antioxidant potential. Likewise, to obtain biologically relevant information, the antioxidant activities of the extracts were evaluated on cellular models implicating the antioxidant activities; this test generally showed that F. communis flower extracts have the highest antioxidant capacities correlated to the highest total phenolic content. The identification of phenolic compounds in F. communis extracts using RP-HPLC revealed that resorcinol, ferulic, and syringic acids together with coumarin were the major molecules. © 2018 Società Botanica Italiana [less ▲]

Detailed reference viewed: 31 (2 ULiège)
Full Text
Peer Reviewed
See detailSynthesis and biochemical evaluation of 2-amino-3-(7-methoxybenzo[d][1,3]dioxol-5-yl)propanoic acid
Etse, Koffi Senam ULiege; Zaragoza, Guillermo; Demonceau, Albert ULiege et al

Poster (2017, December 07)

Heterocyclic 1,3-benzodioxole derivatives are interesting compounds due to their wide range of pharmacological properties.[1] LASSBio 294 (1), for instance, is an alternative therapeutic for cardiac ... [more ▼]

Heterocyclic 1,3-benzodioxole derivatives are interesting compounds due to their wide range of pharmacological properties.[1] LASSBio 294 (1), for instance, is an alternative therapeutic for cardiac dysfunction that increases the contractility of intact and saponin-skinned cardiac muscle of Wistar rats,[2] whereas brasiliamide D (2) and its derivatives display convulsive activity.[3] Analysis of the structure of brasiliamides suggests that they derive from two phenylpropanoic amino acids, phenylalanine and 2-amino-3-(7-methoxybenzo[d][1,3]dioxol-5-yl)propanoic acid (4), and it was suggested that the activity of the brasiliamide family was in part due to the 3- methoxy-4,5-methylene dioxybenzyl group. So, we became interested in the potential biological activity of this amino acid combining a benzodioxole moiety and an amino acid. The synthesis of 4 started from cheap and readily available vanillin (3) using well-established procedures. We then investigated the antioxidant and antiproliferative activity of 4 and some selected precursors. In the DPPH test, 4 showed the highest antiradical activity with IC50 value of 80.91 μM. The enzymatic HRP/H2O2/L012 chemiluminescence assay also revealed a very good antioxidant activity for all compounds with the best activity for 4 (IC50 = 0.36 μM). Cellular tests indicated that most of the tested compounds caused ROS inhibition without cell death. [less ▲]

Detailed reference viewed: 23 (2 ULiège)
Full Text
See detailStudy of the antioxidant action of two analgesic compounds, morphine and propofol, on the activity of MPO and HRP
Nyssen, Pauline ULiege; Mouithys-Mickalad, Ange ULiege; MINGUET, Grégory ULiege et al

Poster (2017, December 05)

Although acute inflammation can efficiently be treated, the treatment of chronic inflammatory pathologies is still a challenge to rise. As marker of inflammation, the enzyme Myeloperoxidase (MPO) is a ... [more ▼]

Although acute inflammation can efficiently be treated, the treatment of chronic inflammatory pathologies is still a challenge to rise. As marker of inflammation, the enzyme Myeloperoxidase (MPO) is a choice target for the establishment of a treatment. Indeed, the uncontrolled release in the extracellular medium of MPO, marker of inflammation, along with the release of ROS, causes severe damages on biological tissues. The modulation of the enzyme activity, by an inhibitor, might constitute an approach to treat excessive inflammation. An interesting pathway is to give a second life to clinical-used molecules, presenting antioxidant and anti-inflammatory properties. According to several studies, morphine and propofol (PPF), which are already known for their analgesic and anesthetic properties, present an antioxidant activity. Therefore, these reducing molecule can potentially pretend to be MPO inhibitors. [less ▲]

Detailed reference viewed: 55 (11 ULiège)