References of "Motte, Patrick"
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See detailAltered metal distribution in the sr45-1 Arabidopsis mutant causes developmental defects
Fanara, Steven ULiege; Schloesser, M.; Hanikenne, Marc ULiege et al

in bioRxiv (2021)

The plant SR (serine/arginine-rich) splicing factor SR45 plays important roles in several biological processes, such as splicing, DNA methylation, innate immunity, glucose regulation and ABA signaling. A ... [more ▼]

The plant SR (serine/arginine-rich) splicing factor SR45 plays important roles in several biological processes, such as splicing, DNA methylation, innate immunity, glucose regulation and ABA signaling. A homozygous Arabidopsis sr45-1 null mutant is viable, but exhibits diverse phenotypic alterations, including delayed root development, late flowering, shorter siliques with fewer seeds, narrower leaves and petals, and unusual numbers of floral organs. Here, we report that the sr45-1 mutant presents an unexpected constitutive iron deficiency phenotype characterized by altered metal distribution in the plant. RNA-Sequencing highlighted severe perturbations in metal homeostasis, phenylpropanoid pathway, oxidative stress responses, and reproductive development. Ionomic quantification and histochemical staining revealed strong iron accumulation in the sr45-1 root tissues accompanied by an iron starvation in aerial parts. We showed that some sr45-1 developmental abnormalities can be complemented by exogenous iron supply. Our findings provide new insight into the molecular mechanisms governing the phenotypes of the sr45-1 mutant. The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. [less ▲]

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See detailThe two copies of the zinc and cadmium ZIP6 transporter of Arabidopsis halleri have distinct effects on cadmium tolerance.
Spielmann, Julien ULiege; Ahmadi, Hassan; Scheepers, Maxime ULiege et al

in Plant, cell & environment (2020)

Plants have the ability to colonize highly diverse environments. The zinc and cadmium hyperaccumulator Arabidopsis halleri has adapted to establish populations on soils covering an extreme range of metal ... [more ▼]

Plants have the ability to colonize highly diverse environments. The zinc and cadmium hyperaccumulator Arabidopsis halleri has adapted to establish populations on soils covering an extreme range of metal availabilities. The A. halleri ZIP6 gene presents several hallmarks of hyperaccumulation candidate genes: it is constitutively highly expressed in roots and shoots and is associated with a zinc accumulation quantitative trait locus. Here, we show that AhZIP6 is duplicated in the A. halleri genome. The two copies are expressed mainly in the vasculature in both A. halleri and A. thaliana, indicative of conserved cis regulation, and acquired partial organ specialization. Yeast complementation assays determined that AhZIP6 is a zinc and cadmium transporter. AhZIP6 silencing in A. halleri or expression in A. thaliana alters cadmium tolerance, but has no impact on zinc and cadmium accumulation. AhZIP6-silenced plants display reduced cadmium uptake upon short-term exposure, adding AhZIP6 to the limited number of Cd transporters supported by in planta evidence. Altogether, our data suggest that AhZIP6 is key to fine-tune metal homeostasis in specific cell-types. This study additionally highlights the distinct fates of duplicated genes in A. halleri. This article is protected by copyright. All rights reserved. [less ▲]

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See detaildi-Cysteine Residues of the Arabidopsis thaliana HMA4 C-Terminus Are Only Partially Required for Cadmium Transport
Ceasar, S. A.; Lekeux, Gilles ULiege; Motte, Patrick ULiege et al

in Frontiers in Plant Science (2020), 11

Cadmium (Cd) is highly toxic to the environment and humans. Plants are capable of absorbing Cd from the soil and of transporting part of this Cd to their shoot tissues. In Arabidopsis, the plasma membrane ... [more ▼]

Cadmium (Cd) is highly toxic to the environment and humans. Plants are capable of absorbing Cd from the soil and of transporting part of this Cd to their shoot tissues. In Arabidopsis, the plasma membrane Heavy Metal ATPase 4 (HMA4) transporter mediates Cd xylem loading for export to shoots, in addition to zinc (Zn). A recent study showed that di-Cys motifs present in the HMA4 C-terminal extension (AtHMA4c) are essential for high-affinity Zn binding and transport in planta. In this study, we have characterized the role of the AtHMA4c di-Cys motifs in Cd transport in planta and in Cd-binding in vitro. In contrast to the case for Zn, the di-Cys motifs seem to be partly dispensable for Cd transport as evidenced by limited variation in Cd accumulation in shoot tissues of hma2hma4 double mutant plants expressing native or di-Cys mutated variants of AtHMA4. Expression analysis of metal homeostasis marker genes, such as AtIRT1, excluded that maintained Cd accumulation in shoot tissues was the result of increased Cd uptake by roots. In vitro Cd-binding assays further revealed that mutating di-Cys motifs in AtHMA4c had a more limited impact on Cd-binding than it has on Zn-binding. The contributions of the AtHMA4 C-terminal domain to metal transport and binding therefore differ for Zn and Cd. Our data suggest that it is possible to identify HMA4 variants that discriminate Zn and Cd for transport. © Copyright © 2020 Ceasar, Lekeux, Motte, Xiao, Galleni and Hanikenne. [less ▲]

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See detailIntertwined metal homeostasis, oxidative and biotic stress responses in the Arabidopsis frd3 mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

in Plant Journal (2020), 102

FRD3 (FERRIC REDUCTASE DEFECTIVE 3) plays a major role in iron (Fe) and zinc (Zn) homeostasis in Arabidopsis. It transports citrate, which enables metal distribution in the plant. A frd3 mutant is dwarf ... [more ▼]

FRD3 (FERRIC REDUCTASE DEFECTIVE 3) plays a major role in iron (Fe) and zinc (Zn) homeostasis in Arabidopsis. It transports citrate, which enables metal distribution in the plant. A frd3 mutant is dwarf and chlorotic and displays constitutive Fe deficiency response and strongly altered metal distribution in tissues. Here, we have examined the interaction between Fe and Zn homeostasis in a frd3 mutant exposed to varying Zn supply. Detailed phenotyping using transcriptomic, ionomic, histochemical and spectroscopic approaches revealed the full complexity of the frd3 mutant phenotype, which resulted from altered transition metal homeostasis, manganese toxicity, oxidative and biotic stress responses. The cell wall played a key role in these processes, as a site for Fe and hydrogen peroxide accumulation, and displayed modified structure in the mutant. Finally, we showed that Zn excess interfered with these mechanisms and partially restored root growth of the mutant, without reverting the Fe deficiency response. In conclusion, the frd3 mutant molecular phenotype is more complex than previously described, and illustrates how the response to metal imbalance depends on multiple signaling pathways. [less ▲]

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See detailH2O2 signaling from mitochondria and chloroplast to the nucleus in the green microalga Chlamydomonas: in vivo and in vitro approaches
Caccamo, Anna ULiege; Nguyen, Ho Thuy Dung; Vertommen, Didier et al

Poster (2019, September 17)

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See detailResolution of the proteome, transcript and ionome dynamics upon Zn re-supply in Zn-deficient Arabidopsis
Arsova, Borjana; Amini, Sahand ULiege; Scheepers, Maxime ULiege et al

E-print/Working paper (2019)

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See detailThe knotty phenotype of the frd3 Arabidopsis mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

Poster (2018, May 17)

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See detailThe knotty phenotype of the frd3 Arabidopsis mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

Poster (2018, February 01)

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See detailWhen splicing meets environmental challenges
Fanara, Steven ULiege; Hanikenne, Marc ULiege; Motte, Patrick ULiege

Conference (2018, February 01)

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See detaildi-Cysteine motifs in the C-terminus of plant HMA4 proteins confer nanomolar affinity for zinc and are essential for HMA4 function in vivo.
Lekeux, Gilles ULiege; Laurent, Clémentine ULiege; Joris, Marine ULiege et al

in Journal of Experimental Botany (2018), 69(22), 5547-5560

The PIB ATPase heavy metal ATPase 4 (HMA4) has a central role in the zinc homeostasis network of Arabidopsis thaliana. This membrane protein loads metal from the pericycle cells into the xylem in roots ... [more ▼]

The PIB ATPase heavy metal ATPase 4 (HMA4) has a central role in the zinc homeostasis network of Arabidopsis thaliana. This membrane protein loads metal from the pericycle cells into the xylem in roots, thereby allowing root to shoot metal translocation. Moreover, HMA4 is key for zinc hyperaccumulation as well as zinc and cadmium hypertolerance in the pseudometallophyte Arabidopsis halleri. The plant-specific cytosolic C-terminal extension of HMA4 is rich in putative metal-binding residues and has substantially diverged between A. thaliana and A. halleri. To clarify the function of the domain in both species, protein variants with truncated C-terminal extension, as well as with mutated di-Cys motifs and/or a His-stretch, were functionally characterized. We show that di-Cys motifs, but not the His-stretch, contribute to high affinity zinc binding and function in planta. We suggest that the HMA4 C-terminal extension is at least partly responsible for protein targeting to the plasma membrane. Finally, we reveal that the C-terminal extensions of both A. thaliana and A. halleri HMA4 proteins share similar function, despite marginally different zinc-binding capacity. [less ▲]

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See detailAdaptation to high zinc depends on distinct mechanisms in metallicolous populations of Arabidopsis halleri
Schvartzman Echenique, Maria Sol ULiege; Corso, Massimiliano; Fataftha, Nazeer et al

in New Phytologist (2018)

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