References of "Millien, Virginie"
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See detailPathobiome of the Lyme disease principal reservoir in southern Quebec (Peromyscus leucopus)
Andre, Adrien; Mouton, alice; Millien, Virginie et al

Conference (2018)

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See detailLiver microbiome of Peromyscus leucopus, a key reservoir host species for emerging infectious diseases in North America
André, Adrien ULiege; Mouton, Alice ULiege; millien, virginie et al

in Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases (2017)

Microbiome studies generally focus on the gut microbiome, which is composed of a large proportion of commensal bacteria. Here we propose a first analysis of the liver microbiomeusing next generation ... [more ▼]

Microbiome studies generally focus on the gut microbiome, which is composed of a large proportion of commensal bacteria. Here we propose a first analysis of the liver microbiomeusing next generation sequencing as a tool to detect potentially pathogenic strains. We used Peromyscus leucopus, the main reservoir host species of Lyme disease in eastern North America, as a model and sequenced V5-V6 regions of the 16S gene from 18 populations in southern Quebec (Canada). The Lactobacillus genus was found to dominate the liver microbiome.We also detected a large proportion of individuals infected by Bartonella vinsonii arupensis, a human pathogenic bacteria responsible for endocarditis, aswell as Borrelia burgdorferi, the pathogen responsible for Lyme disease in North America. We then compared the microbiomes among two P. leucopus genetic clusters occurring on either side of the St. Lawrence River, and did not detect any effect of the host genotype on their liver microbiome assemblage. Finally, we report, for the first time, the presence of B. burgdorferi in a smallmammal host fromthe northern side of the St. Lawrence River, in support of models that have predicted the northern spread of Lyme disease in Canada. [less ▲]

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See detailEffects of parasite and historic driven selection on the diversity and structure of a MHC-II gene in a small mammal species (Peromyscus leucopus) undergoing range expansion
andré, adrien; millien, Virginie; Galan, Maxime et al

in Evolutionary Ecology (2017), DOI 10.1007/s10682-017-9898-z

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See detailStudy of the microbiome from the lyme disease principal reservoir in southern Quebec (Peromyscus leucopus)
Andre, adrien; Mouton, Alice; Millien, Virginie et al

Poster (2016)

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See detailFrom sample hunt to sequence processing, the journey of a biologist
André, Adrien ULiege; Millien, Virginie; Michaux, Johan ULiege

Conference (2015, December 08)

Metabarcoding studies are becoming more and more popular with a field of applications constantly increasing. However, the methods used are sometimes complex and might remain “obscure” for most of us. The ... [more ▼]

Metabarcoding studies are becoming more and more popular with a field of applications constantly increasing. However, the methods used are sometimes complex and might remain “obscure” for most of us. The objective of this presentation is therefore to familiarize people with this field of research by giving an overview of the different steps permitting the achievement of metabarcoding studies. Field work, lab work and bioinformatics will be subsequently detailed and accessibly explained. [less ▲]

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See detailStudy of the microbiome from the lyme disease principal reservoir in southern Quebec (Peromyscus leucopus)
Andre, adrien; mouton, alice; Millien, Virginie et al

Conference (2015, July)

The emergence of the Lyme disease in Southern Quebec appears directly linked to the recent arrival in the region of the rodent Peromyscus leucopus. Indeed, this species is considered to be the principal ... [more ▼]

The emergence of the Lyme disease in Southern Quebec appears directly linked to the recent arrival in the region of the rodent Peromyscus leucopus. Indeed, this species is considered to be the principal reservoir of the bacteria Borrelia Burgdorferei, responsible of the Lyme disease and the recent climatic warming has allowed the mice to colonize higher latitude territories. Other factors, like the "negative control or regulation? " made by pathogens might as well play an important role in the dynamic of expansion of P. leucopus. In our project, we sampled several populations of P. leucopus from the North American border, where the species is thought to be present for 30 years, to the most recently colonized zones, situated approximatively 150km inside of the Quebec province. A characterisation of their microbiome was then performed from their liver, spleen and lungs. Our objectives are threefold: First, we settled a protocol based on NGS methods for the detection of Borrelia Burgdorferei in micro-mammal internal organs. Second, we plan to identify the Borreliosis infection zones and to study their spatio-temporal evolution. Third, we aim to test which of the central-marginal hypothesis or the enemy release hypothesis is best describing the scenario presently happening in southern Quebec concerning the distribution’s expansion of P. leucopus. These information will be of great interest to understand the dynamic of emergence of Borreliosis and to predict the current and future distribution of this disease in order to inform the Canadian health authorities . [less ▲]

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See detailThe microbiome from the Lyme disease principal reservoir host in southern Quebec (Peromyscus leucopus)
André, Adrien ULiege; Mouton, Alice ULiege; Millien, Virginie et al

Poster (2015, June 25)

The emergence of the Lyme disease in Southern Quebec appears directly linked to the recent arrival in the region of the rodent Peromyscus leucopus. Indeed, this species is considered to be the principal ... [more ▼]

The emergence of the Lyme disease in Southern Quebec appears directly linked to the recent arrival in the region of the rodent Peromyscus leucopus. Indeed, this species is considered to be the principal reservoir of the bacteria Borrelia Burgdorferei, responsible of the Lyme disease and the recent climatic warming has allowed the mice to colonize higher latitude territories. Other factors, like the regulation made by pathogens might as well play an important role in the dynamic of expansion of P. leucopus. In our project, we sampled several populations of P. leucopus from the North American border, where the species is thought to be present for 30 years, to the most recently colonized zones, situated approximatively 150km inside of the Quebec province. A characterisation of their microbiome was then performed from their liver, spleen and lungs. Our objectives are threefold: First, we settled a protocol based on NGS methods for the detection of Borrelia Burgdorferei in micro-mammal internal organs. Second, we plan to identify the Borreliosis infection zones and to study their spatio-temporal evolution. Third, we aim to test which of the central-marginal hypothesis or the enemy release hypothesis is best describing the scenario presently happening in southern Quebec concerning the distribution’s expansion of P. leucopus. These information will be of great interest to understand the dynamic of emergence of the Borreliosis and to predict the current and future distribution of this disease in order to inform the Canadian health authorities. [less ▲]

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See detailBiological characteristics of a rodent species in expansion
André, Adrien ULiege; millien, Virginie; Michaux, Johan ULiege

Conference (2014, May)

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See detailÉtude du stress à long terme chez un rongeur (Peromyscus leucopus)
André, Adrien ULiege; gaitan, Jorge; Millien, Virginie et al

Poster (2014)

Detailed reference viewed: 31 (3 ULiège)