References of "Marini Djang'Eing'A, Roland"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailHPLC-UV Method for Standardization of Neorautanenia mitis, an African Plant Used in an Anti-Scabies Ointment
Kagisha, Vedaste ULiege; Frederich, Michel ULiege; Ledoux, Allison ULiege et al

in Revista Brasileira de Farmacognosia (2020)

An HPLC-UV method for standardization of the methanol-soluble extracts from tubers of Neorautanenia mitis (A.Rich.) Verdc., Fabaceae, harvested during different periods and from different sites, is ... [more ▼]

An HPLC-UV method for standardization of the methanol-soluble extracts from tubers of Neorautanenia mitis (A.Rich.) Verdc., Fabaceae, harvested during different periods and from different sites, is described. The chemical fingerprint was established with six identified markers using LC-ESI (+)-MS/MS, including rotenone; the total error was used as validation criterion, the accuracy and risk profiles demonstrated the reliability of the method. The study verified that the major degradation product of rotenone in methanol is dehydrorotenone. The detection range of rotenone was between 40 and 400 μg/ml. The collected samples contained 868–5732 μg/g of rotenone. The concentrations of rotenone in the wild samples from the Ngoma site (5167 ± 565 μg/g) were higher than those registered in the samples from the other sites. No significant differences were observed among the remaining sampling sites, and most of the rotenone was located in the inner part of the tubers (2165 ± 1051 μg/g) when compared with that in their peels (961 ± 320 μg/g). [less ▲]

Detailed reference viewed: 45 (8 ULiège)
Full Text
Peer Reviewed
See detailQuality assessment of three imidazole antiparasitics (albendazole, mebendazole and metronidazole) sold in Benin
YEMOA LOCONON, Achille; AMOUSSA, Ahmed; BONOU, Jacob et al

in International Journal of Pharmacy and Pharmacology (2020), 8(5)

Illicit circuit of medicines disrupts the quality assurance system and the rational use of medicines. Substandard and falsified (SF) medicines present a major risk for the public health, due to lack of ... [more ▼]

Illicit circuit of medicines disrupts the quality assurance system and the rational use of medicines. Substandard and falsified (SF) medicines present a major risk for the public health, due to lack of active ingredient and/or toxicity of certain components. The purpose of this study was to evaluate the quality of three imidazole antiparasitic medicines (albendazole, mebendazole and metronidazole tablets) sold in Benin, to describe the different forms of non-compliances of the medicines sold in the illicit circuit. The samples were collected in the formal and illicit (informal) circuits. The results appeared as follows: irregularities of packaging (25.5% of samples from the illicit circuit); mass uniformity test (14.7% of samples from the illicit circuit were noncompliant); disintegration test (2.1% of samples from the illicit circuit and 3.5% from the formal circuit were non-compliant); identification (all samples were compliant) and assay (47.1% of samples from the informal circuit were non-compliant among which 26.5% of under-dosing and 20.6% of overdose). In sum, there were respectively 38.3% (i.e. 18/47) and 3.5% (i.e. 2/58) of non–compliance in the illicit and formal circuits [less ▲]

Detailed reference viewed: 46 (6 ULiège)
Full Text
Peer Reviewed
See detailPerformance of Sickle SCAN® in the Screening of Sickle Cell Disease in Kisangani Pregnant Women and Attitude towards Results
NEEMA UFOYMUNGU, Yvette; JUAKALI SIHALIKYOLO, Jean Jeannot; Marini Djang'Eing'A, Roland ULiege et al

in Open Journal of Blood Diseases (2020)

In the Democratic Republic of the Congo, the sickle cell trait carriage is estimated at 25%. Routine neonatal screening is not a common practice, leading to a very late diagnosis. In this study, the ... [more ▼]

In the Democratic Republic of the Congo, the sickle cell trait carriage is estimated at 25%. Routine neonatal screening is not a common practice, leading to a very late diagnosis. In this study, the screening of pregnant women was assessed as well as their attitudes. This is an analytical cross-sectional study conducted in 245 pregnant women, sampled by convenience in four hospitals in Kisangani city (Democratic Republic of Congo) and screened using the sickle SCAN® test, from February 1 to July 31, 2019. The sensitivity and specificity of the latter were determined using liquid chromatography coupled with mass spectrometry as the gold standard. The attitudes of 240 pregnant women without previous screening history were assessed upon the announcement of the results. The sensitivity of screening for hemoglobin (Hb) AA and Hb AS was 96.69% and 98.39%, respectively; while the specificities were 99.43% and 96.32%, respectively. The Kappa coefficient (κ) was excellent. Concerning attitudes, Hb SS pregnant women and 55.17% of AS pregnant women worried when the results relating to their hemoglobin status were announced. The sickle SCAN® test was found reliable for sickle cell disease screening in Kisangani. The announcement of the results, mainly positive, raises worry among pregnant woman. Therefore, we recommend the involvement of a clinician psychologist for pre-screening counselling and for results announcement, as well as early newborns and unmarried teenage girls screening [less ▲]

Detailed reference viewed: 42 (10 ULiège)
Full Text
Peer Reviewed
See detailSystematic Screening of Neonatal Sickle Cell Disease with HemoTypeSCTM Kit-Test: Case Study and Literature Review
TEBANDITE KASAI, Emmanuel; BOEMER, François ULiege; Marini Djang'Eing'A, Roland ULiege et al

in Open Journal of Blood Diseases (2020)

HemoTypeSCTM test is a new cheap, faster, and appropriate screening method for neonatal diagnosis of sickle cell disease. The literature reports a few cases of its applicability. This study extends the ... [more ▼]

HemoTypeSCTM test is a new cheap, faster, and appropriate screening method for neonatal diagnosis of sickle cell disease. The literature reports a few cases of its applicability. This study extends the cases study and reviews the available literature. The sample consisted of 99 subjects, including 87 newborns (36 girls and 51 boys; 1.9 - 4.9 kg BW) sampled among 566 babies bone at six hospitals in Kisangani city (Democratic Republic of Congo) during March-April 2019; height infant-adolescents (<18 years); and four adults. Duplicate blood samples of 75 newborns, spotted on filter paper, were transferred to Liège in Belgium for LC-MS test confirmation. Of 99 subjects, 74.74% tested HbAA, 24.26% HbAS and 1% HbSS. The prevalence of HbAS compared to the HbAA phenotype was 15/60 (20%) by HemoTypeSCTM and 14/61 (18.7%) by LC-MS. The concordance between the two methods was 98.3% or a discordance of 1.7%. The findings support the validity of the HemoTypeSCTM test as a sensitive, specific point of care test, cheap and reliable for poor African populations. [less ▲]

Detailed reference viewed: 42 (7 ULiège)
Full Text
Peer Reviewed
See detailStudents’ knowledge on sickle cell disease in Kisangani, Democratic Republic of the Congo
Kambale Kombi, Paul ULiege; Marini Djang'Eing'A, Roland ULiege; ALWORONG'A OPARA, Jean-Pierre et al

in Hematology (2020), 25

Background: Education is needed as an action to reduce morbidity and mortality from sickle cell disease (SCD), an important but largely neglected risk to child survival in most African countries as ... [more ▼]

Background: Education is needed as an action to reduce morbidity and mortality from sickle cell disease (SCD), an important but largely neglected risk to child survival in most African countries as Democratic Republic of Congo (DRC). Objective: To assess the knowledge of Kisangani University students in DRC regarding SCD. Methods: In this non-experimental, cross-sectional study, a validated questionnaire was used to assess the knowledge of 2 112 Kisangani University students in DRC and data were analyzed using SPSS version 20. Results: Most participants, 92.9% (95% confidence interval [CI]: 91.7–93.9) were knowledgeable about SCD and have heard about it through schools and/or universities (46.3%), followed by family (34.5%) and health-care workers (23.5%). Nine hundred and seventy-three (46.1%; 95% CI: 44.0–48.2) and 37.9% (95% CI: 35.9–40.0) subjects indicated, respectively, that SCD is an acquired and hereditary disease. Moreover, 53.6% (95% CI: 51.5–55.7) said that the diagnosis of SCD is made by blood tests, while 46.2% (95% CI: 44.1–48.3) talked about urine tests. About 85.6% were unaware of the risk of children becoming sickle cell patients when both parents have SCD. To prevent SCD, pre-marital screening was cited by only 7.7% (95% CI: 6.6–8.9) of subjects and no measure was known by 25.4% (95% CI: 23.6–27.3). However, 79.6% (95% CI: 77.8–81.3) approved the need of pre-marital screening of SCD. Discussion: This study highlighted that the Kisangani university students’ knowledge regarding SCD is poor and needs to be improved; education programs and motivational campaigns to be enhanced. [less ▲]

Detailed reference viewed: 26 (1 ULiège)
Full Text
Peer Reviewed
See detailFalsification des médicaments et résistance aux antimicrobiens
Marini Djang'Eing'A, Roland ULiege; MBINZE KINDENGE, Jérémie; TAKAISI KIKUNI, Pascal et al

Conference (2020, February 14)

Si l’état de santé des populations en Afrique s’est globalement amélioré durant cette dernière décennie, le continent reste face à de nombreux défis : - grandes questions prioritaires de santé publique ... [more ▼]

Si l’état de santé des populations en Afrique s’est globalement amélioré durant cette dernière décennie, le continent reste face à de nombreux défis : - grandes questions prioritaires de santé publique (malnutrition, maladies des voies respiratoires, maladies diarrhéiques, maladies transmissibles), épidémies émergentes et ré-émergentes (Ebola par exemple) ; - progression de nouvelles pathologies non transmissibles comme le cancer et le diabète, de maladies liées au vieillissement de la population, ainsi que la gestion des catastrophes et aléas naturels ; - déséquilibre en termes de disponibilités de professionnels de la santé, d’équipements et de réponses utilisant les nouvelles technologies. Ce colloque, organisé en collaboration avec le PACODEL, vise à redéfinir le rôle des universités face à cette évolution ainsi que celui de tous les acteurs de la santé, pouvoirs publics, grandes organisations internationales et régionales et sociétés privées. [less ▲]

Detailed reference viewed: 20 (1 ULiège)
Full Text
Peer Reviewed
See detailEvaluation of some quality parameters of crude shea butter produced in Burkina Faso
Goumbri, Wendinmi Bertrand ULiege; Somé, Touridomon Issa; Marini Djang'Eing'A, Roland ULiege et al

Poster (2020, January 31)

Gross composition and physicochemical properties of shea butter (SB) widely vary depending on geographical origin, cultivars and extraction process. This study aims at characterizing the composition of ... [more ▼]

Gross composition and physicochemical properties of shea butter (SB) widely vary depending on geographical origin, cultivars and extraction process. This study aims at characterizing the composition of crude SB collected in Burkina Faso, with a particular interest for the unsaponifiable. Results concerns five samples, all obtained by traditional extraction. The fatty acid (FA) profile (esterified and non-esterified) was analyzed by GC/FID, using an optimized method. Acidity, Iodine (IV) and Peroxide values (PV) were determined by titration. As expected, the main esterified FA (mean values) were: C18:1 (cis-9) (42.07%); C18:0 (40.38%) and (C18:2) n6 (5.61%). The FFA content ranged from 2.27 to 4.17%; the IV from 60.60 to 63.51 and the PV from 6.23 to 9.31 meqO2/kg. The unsaponifiable content was found between 7.24 and 13.50%. In this preliminary study, a great homogeneity was found among the samples. This next step about a large range of samples will be an investigation of some absorbent properties of unsaponifiables using HPLC, UV, IR and FT-Raman spectroscopy, in order to determine relationships between extraction processes and SB composition. [less ▲]

Detailed reference viewed: 43 (5 ULiège)
Full Text
See detailApplication of vibrationnal spectroscopy and chemometrics to access the quality of Locally produced antimalarial medicines in the Democratic Republic of Congo.
Ciza Hamuli, Patient ULiege; Sacre, Pierre-Yves ULiege; Kanyonyo, Martial et al

Poster (2020, January 28)

In recent decades, more than 15% of antimalarials marketed in low- and middle-income countries were of poor quality, in which quinoline derivatives and quinine-based formulations represent 21%. These ... [more ▼]

In recent decades, more than 15% of antimalarials marketed in low- and middle-income countries were of poor quality, in which quinoline derivatives and quinine-based formulations represent 21%. These molecules are used for severe and/or cerebral malaria, as well as for falciparum-resistant malaria treatment [1,2]. This research work deals with the opportunity of using vibrational spectroscopy techniques combined with chemometrics for the quality control of local production of antimalarial drugs. Near Infrared (NIR) spectroscopy was chosen for its low-cost and rapid testing properties as well as the possibility to transfer calibrations to handheld devices to control medicines directly on field [3,4]. The main objective was to develop and validate PLS regression method using NIR spectroscopy to replace the high solvent consuming techniques such as HPLC. Indeed, HPLC analyses are difficult to perform in routine in low- and middle-income countries due to the supply and price of reagents and solvents. Furthermore, liquid chromatography devices often suffer from technical issues needing specialized technicians. The driven idea for the development of the quantitative method was the ease of implementation and use by low skilled staff together with the possibility to analyze different formulations. Multivariate analysis methods based on NIR spectroscopy have been developed and validated for the quality control of quinine sulfate tablets manufactured by a major local manufacturer in the Democratic Republic of Congo (DRC). The samples are prepared by dissolution of quinine salt in HCl 0.5 M that is a cheap and easily available medium in low- and middle-income countries. Calibration and validation samples were prepared by dissolving reference quinine sulfate in the presence of excipients using gravimetric data as reference. After being validated, the method was used to analyze commercial quinine sulfate tablets of 500 mg collected in the local market. The method is considered as valid within the acceptance limits as shown in Fig 1. This approach gives the guarantee that each further measurement of unknown samples is included within the tolerance limits at the +/- 10% acceptance limits with a 5.0 % risk level. [less ▲]

Detailed reference viewed: 60 (3 ULiège)
Full Text
Peer Reviewed
See detailQuantitation of Active Pharmaceutical Ingredient through the Packaging Using Raman Handheld Spectrophotometers: A Comparison Study
Alaoui Mansouri, Mohammed ULiege; Sacre, Pierre-Yves ULiege; Coic, Laureen ULiege et al

in Talanta (2020), 207

Handheld Raman spectroscopy is actually booming. Recent devices improvements aim at addressing the usual Raman spectroscopy issues: fluorescence with shifted‐excitation Raman difference spectroscopy ... [more ▼]

Handheld Raman spectroscopy is actually booming. Recent devices improvements aim at addressing the usual Raman spectroscopy issues: fluorescence with shifted‐excitation Raman difference spectroscopy (SERDS), poor sensitivity with surface enhanced Raman scattering (SERS) and information only about the sample surface with spatially offset Raman spectroscopy (SORS). While qualitative performances of handheld devices are generally well established, the quantitative analysis of pharmaceutical samples remains challenging. The aim of this study was to compare the quantitative performances of three commercially available handheld Raman spectroscopy devices. Two of them (TruScan and IDRaman mini) are equipped with a 785 nm laser wavelength and operate in a conventional backscattering mode. The IDRaman has the Orbital Raster Scanning (ORS) option to increase the analyzed surface. The third device (Resolve) operates with an 830 nm laser wavelength both in backscattering and in SORS modes. The comparative study was carried out on ibuprofen-mannitol-microcrystalline cellulose ternary mixtures. The concentration of ibuprofen ranged from 24 to 52 % (w/w) while the proportions of the two excipients were varied to avoid cross-correlation as much as possible. Analyses were performed either directly through a glass vial or with the glass vial in an opaque polypropylene flask, using a validated FT-NIR spectroscopy method as a reference method. Chemometric analyses were carried out with the Partial Least Squares Regression (PLS-R) algorithm. The quantitative models were validated using the total error approach and the ICH Q2 (R1) guidelines with +/- 15% as acceptance limits. [less ▲]

Detailed reference viewed: 195 (92 ULiège)
Full Text
Peer Reviewed
See detailComorbidity of sickle cell trait and albinism: a cross-sectional survey in the Democratic Republic of the Congo.
Kambale-Kombi, Paul; Marini Djang'Eing'A, Roland ULiege; Alworong'a Opara, Jean-Pierre et al

in Pan African Medical Journal (2020), 35

INTRODUCTION: Sickle Cell Disease (SCD) and albinism are both recessive hereditary diseases in human kind with a high prevalence in sub-Saharan Africa. This study aimed to determinate the prevalence of ... [more ▼]

INTRODUCTION: Sickle Cell Disease (SCD) and albinism are both recessive hereditary diseases in human kind with a high prevalence in sub-Saharan Africa. This study aimed to determinate the prevalence of sickle cell trait in people living with albinism (PLA). METHODS: a cross-sectional descriptive survey was conducted in PLA attending the "Hôpital du Cinquantenaire de Kisangani". In total, by non-probabilistic convenience sampling, 82 albinos and 139 non-albinos and without any antecedents of albinism in their family were included, selected from students in the Faculty of Medicine and Pharmacy at the University of Kisangani. Blood samples were collected on "dried blood spot" and analyzed by mass spectrometry at CHU of Liège. Data were entered into an Excel file and analysed on SPSS 20.0 (Chicago, IL). RESULTS: forty-six of the 82 albinos (56.1%) were female and 43.9% male with a sex ratio of 1.28. Among albinos, 18.3% had hemoglobin AS (HbAS) and 81.7% hemoglobin AA (HbAA) compared to 18% of subjects with hemoglobin AS and 82% hemoglobin AA in the control group. The difference was not statistically significant (Chi-square=0.003, ddl=1, p=0.9544). CONCLUSION: this study highlighted that the prevalence of the sickle cell trait is high among people living with albinism, but does not differ from that observed in non-albinos in the Democratic Republic of the Congo. It is therefore important to raise awareness among this category of people about sickle cell disease and the importance of its premarital screening. [less ▲]

Detailed reference viewed: 35 (3 ULiège)
Full Text
Peer Reviewed
See detailQuality of antimalarials in Kinshasa peri-urban areas with regard to local pharmaceutical legislation and regulation
Mavungu Landu, Don Jethro ULiege; Frederich, Michel ULiege; Manzambi Kuwekita, Joseph et al

in International Health (2020)

Background: In the context of old pharmaceutical legislation and regulations not adapted to current realities, the aim of the present studywas to evaluate the existing pharmaceutical system in peri-urban ... [more ▼]

Background: In the context of old pharmaceutical legislation and regulations not adapted to current realities, the aim of the present studywas to evaluate the existing pharmaceutical system in peri-urban areas of Kinshasa. Methods: A prospective study was carried out during the period 2016–2018. The most used antimalarial medicines were identified through household and pharmaceutical establishment surveys. The samples of the obtained medicines were assayed with generic separation methods using the high-performance liquid chromatography technique coupled to a diode array detector. The registration status was checked for 126 antimalarial brand names. A characterization was carried out in 196 pharmaceutical establishments on the basis of standards set out by the Ministry of Health. Results: Of the 75 samples assayed, 19% (14/75)were non-compliant. Of the 124 brand names, 46.0% (57/124) were unlicensed and 14.5% (18/124) had an expired licence. Of the 196 pharmaceutical establishments, only 2 (1.0%) had an authorization to practice, none met all the Ministry of Health minimum standards and 24.5% (48/196) met the World Health Organization Guidelines for the Storage of Essential Medicines and Other Health Commodities. Conclusions: More resources should be mobilized to apply regulator sanctions. [less ▲]

Detailed reference viewed: 89 (28 ULiège)
Full Text
Peer Reviewed
See detailBlood Lead, Urinary Lead and Urinary δ-Aminolevulinic Acid Levels among Children Living in Kinshasa, D R Congo: A Pilot Biomonitoring Study,
Mputu, MLC; Ndelo, MP; Marini Djang'Eing'A, Roland ULiege et al

in Journal of toxicology and Risk Assessment (2019), 5(2),

Lead exists naturally in the earth's crust and it is widely used as a heavy metal. It is an environment toxicant that may deleteriously affect nervous, hematopoietic, skeletal, renal, endocrine and ... [more ▼]

Lead exists naturally in the earth's crust and it is widely used as a heavy metal. It is an environment toxicant that may deleteriously affect nervous, hematopoietic, skeletal, renal, endocrine and reproductive systems. So, exposure to lead in the environment continues to be a serious public health problem for all ages. Children are particularly susceptible to lead poisoning. They absorb more lead from their environment and their developing central nervous systems are vulnerable to the toxic. Fifty percent of children aged from 0 to 17-years-old presented blood lead levels above 100 μg/L and forty three percent of the same population presented blood lead levels above 50 μg/L. There was no correlation between urinary lead level and D-ALAU (urinary δ-aminolevulinic acid) with lead blood levels. But it was observed a small correlation between urinary lead and blood lead concentrations. [less ▲]

Detailed reference viewed: 38 (10 ULiège)
Full Text
Peer Reviewed
See detailEvaluation of some quality parameters of crude shea butter produced in Burkina Faso
Goumbri, Wendinmi Bertrand ULiege; Somé, Touridomon Issa; Marini Djang'Eing'A, Roland ULiege et al

Poster (2019, December 11)

Gross composition and physicochemical properties of shea butter (SB) widely vary depending on geographical origin, cultivars and extraction process. This study aims at characterizing the composition of ... [more ▼]

Gross composition and physicochemical properties of shea butter (SB) widely vary depending on geographical origin, cultivars and extraction process. This study aims at characterizing the composition of crude SB collected in Burkina Faso, with a particular interest for the unsaponifiable. Results concerns five samples, all obtained by traditional extraction. The fatty acid (FA) profile (esterified and non-esterified) was analyzed by GC/FID, using an optimized method. Acidity, Iodine (IV) and Peroxide values (PV) were determined by titration. As expected, the main esterified FA (mean values) were: C18:1 (cis-9) (42.07%); C18:0 (40.38%) and (C18:2) n6 (5.61%). The FFA content ranged from 2.27 to 4.17%; the IV from 60.60 to 63.51 and the PV from 6.23 to 9.31 meqO2/kg. The unsaponifiable content was found between 7.24 and 13.50%. In this preliminary study, a great homogeneity was found among the samples. This next step about a large range of samples will be an investigation of some absorbent properties of unsaponifiables using HPLC, UV, IR and FT-Raman spectroscopy, in order to determine relationships between extraction processes and SB composition. [less ▲]

Detailed reference viewed: 35 (3 ULiège)
Full Text
Peer Reviewed
See detailGlobal access to quality-assured medical products: the Oxford Statement and call to action
Newton, Paul; Bond, Katherine; Sacre, Pierre-Yves ULiege et al

in Lancet Global Health (2019), 7(PE1602-E1611),

Detailed reference viewed: 29 (7 ULiège)
Full Text
Peer Reviewed
See detailSyndrome thoracique aigu chez une gestante AS méconnue: à propos d’un cas
NEEMA UFOYMUNGU, Yvette; BOSENGE NGUMA, Jean Didier; KATENGA BOSUNGA, Gédéon et al

in Pan African Medical Journal (2019)

We report a case of acute thoracic syndrome diagnosed in a Congolese pregnant woman in whom sickle-cell trait was detected after admission to hospital for fever and respiratory symptoms. Paraclinical ... [more ▼]

We report a case of acute thoracic syndrome diagnosed in a Congolese pregnant woman in whom sickle-cell trait was detected after admission to hospital for fever and respiratory symptoms. Paraclinical examinations performed to detect the cause of the disease were unhelpful. Chest X-ray showed infiltrates at the base of the lungs. Sickle SCAN test kit confirmed by liquid chromatography associated with mass spectrometry showed that the patient had sickle cell trait Symptoms were quite refractory to treatment. A favorable outcome was observed after expulsion of the fetus. Clinicians should suspect acute thoracic syndrome in pregnant women with sickle-cell trait because outcome can be favorable in the postpartum period. [less ▲]

Detailed reference viewed: 26 (1 ULiège)
Full Text
Peer Reviewed
See detailComparing the qualitative performances of handheld NIR and Raman spectrophotometers for the detection of falsified pharmaceutical products
Ciza Hamuli, Patient ULiege; Sacre, Pierre-Yves ULiege; Waffo Tchounga, Christelle ULiege et al

in Talanta (2019), 202

Over the last decade, the growth of the global pharmaceutical market has led to an overall increase of substandard and falsified drugs especially on the African market (or emerging countries). Recently ... [more ▼]

Over the last decade, the growth of the global pharmaceutical market has led to an overall increase of substandard and falsified drugs especially on the African market (or emerging countries). Recently, several methods using handheld/portable vibrational spectroscopy have been developed for rapid and on-field drug analysis. The objective of this work was evaluate the performances of various NIR and Raman handheld spectrophotometers in specific brand identification of medicines through their primary packaging. Three groups of drug samples (artemether-lumefantrine, paracetamol, and ibuprofen) were used in tablet or capsule forms. In order to perform a critical comparison, the analytical performances of the two analytical systems was compared statistically using three methods: hierarchical clustering algorithm (HCA), data-driven soft independent modeling of class analogy (DD-SIMCA) and hit quality index (HQI). The overall results show good detection abilities for NIR systems compared to Raman systems based on Matthews’s correlation coefficients, generally close to one. Raman systems are less sensitive to the physical state of the samples than the NIR systems, it also suffers of the auto-fluorescence phenomenon and the signal of highly dosed active pharmaceutical ingredient (e.g. paracetamol or lumefantrine) may mask the signal of low-dosed and weaker Raman active compounds (e.g. artemether). Hence, Raman systems are less effective for specific product identification purposes but are interesting in the context of falsification because they allow a visual interpretation of the spectral signature (presence or absence of API). [less ▲]

Detailed reference viewed: 112 (56 ULiège)
Full Text
See detailIdentification of pharmaceutical products using Handheld NIR and Raman spectrophotometers.
Ciza Hamuli, Patient ULiege; Sacre, Pierre-Yves ULiege; Waffo Tchounga, Christelle Ange ULiege et al

Scientific conference (2019, June 14)

Abstract: Context: Over the last decade, the growth of the global pharmaceutical market has led to an overall increase of substandard and falsified drugs especially on the African market (or emerging ... [more ▼]

Abstract: Context: Over the last decade, the growth of the global pharmaceutical market has led to an overall increase of substandard and falsified drugs especially on the African market (or emerging countries). Recently, several methods using vibrational spectroscopy have been developed for rapid and on-field drug analysis. Objective: The objective of this work was to evaluate the identification performances of handheld near infrared (NIR) and Raman systems. Methods: The formulations of artemether-lumefantrine were used in tablet dosage forms. In order to perform a critical comparison, the analytical performances of the two analytical systems were compared statistically using the method of data-driven soft independent modeling of class analogy (DD-SIMCA). Results: The overall results show good detection abilities for NIR systems compared to Raman systems based on Matthews’s correlation coefficients, generally close to one Raman systems are less sensitive to the physical state of the samples than the NIR systems, because of the strong influence of the auto-fluorescence phenomenon, the signal of highly-dosed active pharmaceutical ingredient (lumefantrine) masking the signal of low-dose and weak Raman scatterers API (e.g. artemether). Conclusion: Hence, Raman systems are less effective for authentication purposes but are interesting in the context of falsification because they allow a visual interpretation of the spectral signature (presence or absence of API). [less ▲]

Detailed reference viewed: 60 (5 ULiège)
Full Text
Peer Reviewed
See detailComparison of hyperspectral imaging techniques for the elucidation of falsified medicines composition
Coic, Laureen ULiege; Sacre, Pierre-Yves ULiege; Dispas, Amandine ULiege et al

in Talanta (2019), 198

Hyperspectral imaging has shown a high potential to analyze falsifications of solid pharmaceutical products since the last decade. Thanks to the non-destructive, ecological and non-invasive properties, it ... [more ▼]

Hyperspectral imaging has shown a high potential to analyze falsifications of solid pharmaceutical products since the last decade. Thanks to the non-destructive, ecological and non-invasive properties, it is a preferred technique for these kinds of applications. Moreover, thanks to the spectroscopic properties, it is possible to detect as well organic compounds as inorganic compounds in a single analysis. Therefore, we recommend using it as second-line laboratory analysis technique. Raman microscopy and Fourier Transform Infrared (FT-IR) microscopy are two interesting techniques that are complementary. In this study, the potential of the two hyperspectral imaging techniques is evaluated to elucidate the composition of falsified antimalarial tablets. Hyperspectral data are analyzed by Multivariate Curve Resolution-Alternating Least Square (MCR-ALS). The results obtained from this study show that Raman hyperspectral imaging seems to be more suited to detect low dosed compounds possibly due to a smallest sampling volume. It has been also possible to link formulations of falsified samples of two different brands. [less ▲]

Detailed reference viewed: 63 (25 ULiège)
Full Text
Peer Reviewed
See detailCOMPARATIVE STUDY OF ACTIVE PHARMACEUTICAL INGREDIENT QUANTITATION THROUGH THE PACKAGING USING HANDHELD SPECTROPHOTOMETERS BASED ON CONVENTIONAL AND SPATIALLY OFFSET RAMAN SCATTERING
Alaoui Mansouri, Mohammed ULiege; Sacre, Pierre-Yves ULiege; Coic, Laureen ULiege et al

Conference (2019, May 20)

Handheld Raman spectroscopy is actually booming. Recent devices aim at addressing the usual Raman spectroscopy issues: fluorescence with shifted‐excitation Raman difference spectroscopy (SERDS), poor ... [more ▼]

Handheld Raman spectroscopy is actually booming. Recent devices aim at addressing the usual Raman spectroscopy issues: fluorescence with shifted‐excitation Raman difference spectroscopy (SERDS), poor sensitivity with surface enhanced Raman scattering (SERS) and only surface information with spatially offset Raman spectroscopy (SORS). While qualitative performances of handheld devices are generally well established, the quantitative analysis of pharmaceutical samples remain challenging. The aim of this study was to compare the quantitative performances of three commercially available handheld Raman spectroscopy devices. Two devices (ThermoFisher’s Truscan and Ocean Optic’s IDRaman mini) are equipped with a 785 nm laser wavelength and operate in a conventional backscattering mode. The IDRaman has the Orbital Raster Scanning (ORS) option to increase the analyzed surface. The third device (Agilent’s Resolve) operates with a 830 nm laser wavelength both in a backscattering mode and in the SORS mode. The comparative study was carried out on ibuprofen-mannitol-microcrystalline cellulose ternary mixtures. The concentration of ibuprofen ranged from 24 to 52 % (m/m) while proportions of the two excipients were varied to avoid cross-correlation as much as possible. Analyses were performed directly through a glass vial and with the glass vial in a polypropylene flask using a validated FT-NIR spectroscopy method as a reference method. Chemometric analyses were carried out with the Partial Least Squares Regression (PLS-R) algorithm. The quantitative models were validated using the total error approach and the ICH Q2R1 guidelines with +/- 15% as acceptance limits. [less ▲]

Detailed reference viewed: 72 (12 ULiège)
Full Text
Peer Reviewed
See detailField survey to evaluate the prevalence of poor quality anti-infective medicines in Cameroon
Waffo Tchounga, Christelle Ange ULiege; Ciza Hamuli, Patient ULiege; Sacre, Pierre-Yves ULiege et al

Conference (2019, May 20)

Poor quality medicines pose a threat to all health systems. It is obvious that they have harmful consequences not only from the point of view of public health, but also from the economic and socio ... [more ▼]

Poor quality medicines pose a threat to all health systems. It is obvious that they have harmful consequences not only from the point of view of public health, but also from the economic and socio-economic point of view [1]. Over the past 15 years, substandards and falsified drugs have received increasing attention in scientific publications. However, there is little reliable data determining their prevalence with accuracy due to the scarcity of well-designed studies identified as having good methodological quality as well as representative sampling strategy [2-4]. In this context, we have decided to evaluate the prevalence of poor quality anti-infective medicines in two Cameroon areas (Yaoundé and Douala), inspired by the Medicines Quality Assessment Reporting Guidelines (MEDGUARG) [3] and WHO recommendations [4] for the sampling strategy and the methodology. Our study will focus on the formal private sector. Pharmacies as well as drugs products will be sampled by a stratified random sampling strategy. The study will focus on two anti-infective medicines (ciprofloxacin and metronidazole 500mg tablets) in tablets, from 96 outlets in the cities of Yaoundé and Douala that are the two main cities (Yaoundé and Douala) of Cameroon representing almost 70% of private outlets of the country. Mystery shoppers will collect samples using a specific scenario. As a prelude to our field study, screening and dosage methods have to be developed and validated in Liège University. They se methods consist of vibrational spectroscopy (near infrared and Raman spectroscopy) as first screening techniques and HPLC for identification and assay. For vibrational spectroscopy, qualitative models will be developed for identification using chemometric tools. HPLC methods will be validated following the total error approach using accuracy profile as decision tool. The medicines collected will be first analysed visually (physical appearance tests), then field methods will be implemented (screening methods: Paper Analytical Devices (PADs), handled NIR device). Finally laboratory testing (assay and confirmation methods: HPLC reference method and pharmacotechnical tests) will be performed at LANACOME (Yaoundé, Cameroon). Suspect and unusual samples will be transported to Liège University for further analyses. All these methods will be applied according to a decision tree based on observed facts. The study will be submitted to the ethics committee of the Ministry of Health in Cameroon. An accurate and fast HPLC method for identification and quantification of both metronidazole and ciprofloxacin has been developed. Identification models for some brands of ciprofloxacin and metronidazole using handled NIR and Raman devices has been developed before implementation on field. This study will allow us to evaluate not only the prevalence of poor quality anti-infective medicines marketed in Cameroon but also outlets dispensing substandard and falsified medicines. They will be distinguished into sub-standard, degraded or falsified and classified according to their country of origin, manufacturer and city of sampling. The results will be notified to the drug regulatory authority in Cameroon and if poor quality medicines are detected, we will proceed with an alert to the WHO Global Surveillance System. The estimation of the prevalence of counterfeit and falsified anti-infective medicines would be extrapolated to the entire population and depending on the information obtained, evaluate the patient health risk exposed to substandard and falsified anti-infective medicines and develop capacity-building interventions in the fight against poor quality medicines. [less ▲]

Detailed reference viewed: 33 (6 ULiège)