References of "Mainil, Jacques"
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See detailNew Bacteriophages against Emerging Lineages ST23 and ST258 of Klebsiella pneumoniae and Effi cacy Assessment in Galleria mellonella Larvae
Thiry, Damien ULiege; Passet, Virginier; Danis-Wlodarczyk, Katarzyna et al

in Viruses (2019), 11(5),

Klebsiella pneumoniae is a bacterial pathogen of high public health importance. Its polysaccharide capsule is highly variable but only a few capsular types are associated with emerging pathogenic ... [more ▼]

Klebsiella pneumoniae is a bacterial pathogen of high public health importance. Its polysaccharide capsule is highly variable but only a few capsular types are associated with emerging pathogenic sublineages. The aim of this work is to isolate and characterize new lytic bacteriophages and assess their potential to control infections by the ST23 and ST258 K. pneumoniae sublineages using a Galleria mellonella larvae model. Three selected bacteriophages, targeting lineages ST258 (bacteriophages vB_KpnP_KL106-ULIP47 and vB_KpnP_KL106-ULIP54) and ST23 (bacteriophage vB_KpnP_K1-ULIP33), display specificity for capsular types KL106 and K1, respectively. These podoviruses belong to the Autographivirinae subfamily and their genomes are devoid of lysogeny or toxin-associated genes. In a G. mellonella larvae model, a mortality rate of 70% was observed upon infection by K. pneumoniae ST258 and ST23. This number was reduced to 20% upon treatment with bacteriophages at a multiplicity of infection of 10. This work increases the number of characterized bacteriophages infecting K. pneumoniae and provides information regarding genome sequence and efficacy during preclinical phage therapy against two prominent sublineages of this bacterial species. [less ▲]

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See detailAssessment of two selective agar media to isolate colistin-resistant bovine Escherichia coli: Correlation with minimal inhibitory concentration and presence of mcr genes
Thiry, Damien ULiege; Berrah, Anis ULiege; Evrard, Julien et al

in Journal of microbiological methods (2019), 159

The identification of colistin-resistant enterobacteria in veterinary medicine is impaired by the absence of first-line reliable phenotypic assay. The purpose of this study was to assess two selective ... [more ▼]

The identification of colistin-resistant enterobacteria in veterinary medicine is impaired by the absence of first-line reliable phenotypic assay. The purpose of this study was to assess two selective agar media for the detection of colistin-resistant bovine pathogenic Escherichia coli. A total of 158 E. coli (46 R <resistant>, 96 I <intermediate> and 16 S <sensitive> at the disk diffusion assay) isolated between 2013 and 2018 from <3 month-old calves suffering enteritis or septicaemia, were (i) tested by the broth dilution assay to determine colistin Minimal Inhibitory Concentrations (MIC); (ii) streaked on CHROMID® Colistin_R and CHROMagar™ COL-APSE agar plates; (iii) submitted to a pentaplex PCR to identify the presence of mcr-1 to mcr-5 genes. Of the 92 E. coli growing on both agar media, 90 had a MIC > 2.0 μg/ml as had the 3 E. coli that grew only on the CHROMID® Colistin_R agar medium and one E. coli that grew on neither agar media. Therefore, the positive predictive values of the CHROMID® Colistin_R and CHROMagar™ COL-APSE agar media were both 0.98 whereas their negative predictive values were 0.98 and 0.94, respectively. Also noteworthy 43 of the 46 R isolates had a MIC > 2.0 μg/ml and grew on both selective media as did half of the 96 I isolates and only 1 of the S isolates. Conversely, only 30 of the 90 isolates that grew on both agar media and with a MIC > 2.0 μg/ml tested positive for the mcr-1 or mcr-2 genes with the pentaplex PCR. These two selective agar media can be used to reliably detect colistin-resistant E. coli. Positive growth was highly correlated with R results at the disk diffusion assay, but not with the presence of mcr genes. Copyright © 2019. Published by Elsevier B.V. [less ▲]

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See detailCHROMAgar Mastitis medium: assessment of the new formulation and comparison with the previous one
DIDESSE, Elsa; Duprez, Jean-Noël ULiege; Mainil, Jacques ULiege et al

Poster (2018, October 26)

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See detailIdentification of unconventional O serotypes of enteropathogenic (EPEC) Escherichia coli from intestinal contents of healthy cattle at slaughterhouse
Ikeda, Rie; Habets, Audrey ULiege; Duprez, Jean-Noël ULiege et al

Poster (2018, October 26)

Escherichia coli producing the attaching-effacing (AE) lesion are called enteropathogenic E. coli (EPEC) and are responsible for diarrhoea in animals and humans. EPEC are subdivided into typical (t) EPEC ... [more ▼]

Escherichia coli producing the attaching-effacing (AE) lesion are called enteropathogenic E. coli (EPEC) and are responsible for diarrhoea in animals and humans. EPEC are subdivided into typical (t) EPEC producing the “Bundle Forming Pili” type 4 fimbriae and isolated from humans, and atypical (a) EPEC not producing the BFP and isolated from humans and animals, including diarrheic young calves and healthy adult cattle. tEPEC and several aEPEC belong to specific serotypes, but different aEPEC can also belong to classical Shigatoxigenic E. coli (STEC) O serotypes (O26, O103, O111, O121, O145, O157, O165) and derive from STEC after loss of genes encoding the Shiga toxins. In 2014 several hundreds of EPEC from healthy cattle at two slaughterhouses in Wallonia, but the prevalence of the classical STEC O serotypes was low and all were negative for the recently described O80 EPEC serotype. The aim of this study was to identify 6 other unconventional O serotypes (O123/186, O146, O156, O177, O182, O183) recently identified by PCR in different bovine aEPEC and STEC by one of us, amongst 312 EPEC isolated at slaughterhouse that previously tested negative, applying two triplex PCRs either for the O146_O182_O183 or for the O123/186_O156_O177 serotypes. So far the first triplex PCR identified 1 O146-positive and 8 O182-positive EPEC. The second triplex PCR identified 30 O156. The further steps of this study are: (i) the identification of still other unconventional serogroups among EPEC (ii) the identification of their H antigen-encoding genes; (iii) the comparison of these bovine EPEC between themselves, with EPEC from diarrheic calves and with STEC from cattle and humans belonging to the same serotypes. [less ▲]

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See detailIsolation of sero-pathotype specific bacteriophages against unconventional Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli from diarrheic calves.
Habets, Audrey ULiege; Duprez, Jean-Noël ULiege; Iguchi, Atsushi et al

Conference (2018, October 26)

Escherichia coli producing the Shiga toxins (STEC) and/or the attaching-effacing (AE) lesion (EPEC) cause enteritis and (bloody) diarrhoea in young calves and in humans. STEC and EPEC can belong 7 ... [more ▼]

Escherichia coli producing the Shiga toxins (STEC) and/or the attaching-effacing (AE) lesion (EPEC) cause enteritis and (bloody) diarrhoea in young calves and in humans. STEC and EPEC can belong 7 serogroups frequently identified worldwide: O26, O103, O111, O121, O145, O157 and O165. Beside these classical “gang of 7” serogroups, unconventional serogroups can be identified as previously demonstrated with the O80 EPEC. The use of active and specific bacteriophages as biocontrol agents seems to be a promising alternative against unconventional STEC/EPEC and have been employed for diagnostic. The first aim of this project was to identify those 6 unconventional serogroups among 76 STEC and EPEC isolated between 2008 and 2015 from diarrheic calves at ARSIA. Two triplex PCRs have been applied either for the O146_O182_O183 serogroups or for the O123/186_O156_O177 serogroups. The second objective of this project was to isolate specific bacteriophages against these unconventional serogroups from wastewater or farm slurry. So far, the first triplex PCR identified 4 O182-positive and 2 O183-positive STEC and EPEC. The second triplex PCR identified 10 O123-186-positive, 2 O156-positive and 13 O177-positive STEC and EPEC. A total of 4 potentially specific bacteriophages respectively active against O5, O123, O146, O186 were obtained. The further steps of this study will be to perform: (i) the PFGE profile comparison of the calf and healthy cattle PCR-positive STEC and EPEC between themselves and with human STEC and EPEC; (ii) the identification of still other unconventional serogroups among STEC and EPEC from diarrheic calves; (iii) the host range spectrum of the 4 potentially specific bacteriophages. [less ▲]

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See detailIdentification of unconventional Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli from diarrheic calves.
Habets, Audrey ULiege; Duprez, Jean-Noël ULiege; Iguchi, Atsushi et al

Poster (2018, October)

Introduction Escherichia coli producing the Shiga toxins (STEC) and/or the attaching-effacing (AE) lesion (AE-STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also ... [more ▼]

Introduction Escherichia coli producing the Shiga toxins (STEC) and/or the attaching-effacing (AE) lesion (AE-STEC) cause enteritis and (bloody) diarrhoea in young calves and in humans, and are also present in the intestines of healthy cattle. AE-STEC in humans can belong to scores of O serogroups with 7 most frequent and pathogenic ones (O157, O26, O103, O111, O121, O145 and O165). Conversely the majority of AE-STEC in young calves belong to a narrower range of serogroups (O26, O111, O118, O5). Besides AE-STEC, E. coli producing only the AE lesion (EPEC) are also frequently associated with diarrhoea in young calves. Half of them belong to the O26 and O80 serogroups,, but the serotypes of the second half of isolates remain unidentified (Fakih et al. 2017; Thiry et al., 2018). Materials and Methods Different unconventional serogroups (O123/186, O156, O177, O182, O183) were identified by PCR in 5 STEC or EPEC that tested negative for the pre-cited 12 serogroups (Iguchi et al., 2015). The aim of this study was therefore to identify those 5 serogroups and the O146 serogroup amongst 76 AE-STEC, STEC and EPEC isolated between 2008 and 2015 from diarrheic calves at ARSIA (Fakih et al., 2017). Two triplex PCRs were applied either for the O146_O182_O183 serogroups or for the O123/186_O156_O177 serogroups. Discussion So far the first triplex PCR identified 4 O182-positive and 2 O183-positive STEC and EPEC. These 6 PCR-positive isolates were confirmed by either uniplex PCR. Two O182-positive isolates were AE-STEC and 2 were EPEC whereas the two O183-positive isolate were STEC. The second triplex PCR identified 10 O123-186-positive, 2 O156-positive and 13 O177-positive STEC and EPEC. These 25 PCR-positive isolates were confirmed by the three uniplex PCR. One O123/186-positive isolate was AE-STEC and 9 were EPEC. One O156-positive isolate was AE-STEC and one was STEC and finally, 12 O177-positive isolates were EPEC and 1 was STEC. The further steps of the work are: (i) the identification of the same 6 serogroups amongst a collection of (AE-)STEC and EPEC isolated from healthy cattle at slaughterhouse; (ii) the identification of still other serogroups amongst the remaining untyped calf (AE-)STEC and EPEC; (iii) the comparison of the calf-positive STEC and EPEC with human (AE-)STEC and EPEC belonging to the same serogroups. References Fakih, I., Thiry, D., Duprez, J. N., Saulmont, M., Iguchi, A., Piérard, D., … Mainil, J. G. (2017). Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC. Veterinary Microbiology, 202, 16–22. Iguchi, A., Iyoda, S., Seto, K., Morita-Ishihara, T., Scheutz, F., & Ohnishi, M. (2015). Escherichia coli O-genotyping PCR: A comprehensive and practical platform for molecular O serogrouping. Journal of Clinical Microbiology, 53(8), 2427–2432. https://doi.org/10.1128/JCM.00321-15 Thiry, D., De Rauw, K., Takaki, S., Duprez, J. N., Iguchi, A., Piérard, D., … Mainil, J. G. (2018). Low prevalence of the “gang of seven” and absence of the O80:H2 serotypes among Shigatoxigenic and enteropathogenic Escherichia coli (STEC and EPEC) in intestinal contents of healthy cattle at two slaughterhouses in Belgium in 2014. Journal of Applied Microbiology, 124(3), 867–873. [less ▲]

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See detailIdentification of serotypes of enteropathogenic (EPEC) Escherichia coli isolated from diarrheic calves
Habets, Audrey ULiege; Duprez, Jean-Noël ULiege; Iguchi, Atsuchi et al

Poster (2018, October)

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See detailPresence Of Enterohemorrhagic Escherichi coli (Ehec) On Flemish Dairy Cattle Farms
Engelen, F.; Mainil, Jacques ULiege; Thiry, Damien ULiege et al

Poster (2018, May)

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See detailComparison Of The Emerging O80:H2 Shiga Toxin-Producing And Enteropathogenic Escherichia coli From Human and Bovine Origin
De Rauw, K.; Thiry, Damien ULiege; Caljon, B. et al

Poster (2018, May)

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See detailImplementation of the Galleria mellonella larvae model to evaluate phage therapy against Klebsiella pneumoniae
Thiry, Damien ULiege; Passet, V.; Danis-Wlodarczyk, K. et al

Conference (2018, March)

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See detailLow prevalence of the 'gang of seven' and absence of the O80:H2 serotypes among Shigatoxigenic and enteropathogenic Escherichia coli (STEC and EPEC) in intestinal contents of healthy cattle at two slaughterhouses in Belgium in 2014.
Thiry, Damien ULiege; De Rauw, Klara; Tataki, Shino et al

in Journal of Applied Microbiology (2018), 124(3), 867-873

The purpose of this survey was to estimate the respective prevalence of the 'gang of seven' and 'non-gang of seven' serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the ... [more ▼]

The purpose of this survey was to estimate the respective prevalence of the 'gang of seven' and 'non-gang of seven' serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the O80:H2 serotype in 245 intestinal contents collected at two slaughterhouses in Belgium in 2014. [less ▲]

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See detailCharacteristics of Shiga toxin producing- and enteropathogenic Escherichia coli of the emerging serotype O80:H2 isolated from humans and diarrhoeic calves in Belgium
De Rauw, K.; Thiry, Damien ULiege; Caljon, B. et al

in Clinical Microbiology and Infection (2018)

Objectives: Recently a highly virulent Escherichia coli O80:H2 pathotype carrying Shiga toxin genes, the intimin subtype eaeξ, and genes associated with the extraintestinal pathogenic E. coli (ExPEC) pS88 ... [more ▼]

Objectives: Recently a highly virulent Escherichia coli O80:H2 pathotype carrying Shiga toxin genes, the intimin subtype eaeξ, and genes associated with the extraintestinal pathogenic E. coli (ExPEC) pS88 plasmid was described in France. In this study we examine the relatedness of Belgian E. coli O80:H2 isolated from humans and diarrhoeic calves as well their similarities with the French pathotype. Methods: Eighteen Belgian E. coli O80:H2 strains (nine human Shiga toxin-producing E. coli (STEC) (2008–2016), two bovine STEC (1987) and seven bovine atypical enteropathogenic E. coli (aEPEC) (2009–2015)) were characterized with conventional PCR, disc diffusion susceptibility testing and whole genome sequencing. Results: Only nine sporadic human STEC O80:H2 cases have been detected in Belgium. All patients were female, just two of them suffered from haemolytic uremic syndrome. All studied strains had the eaeξ subtype, belonged to the multi-locus sequence type ST-301, and carried virulence genes associated with the type III secretion system and effectors not encoded by the locus of enterocyte effacement (LEE). Multiple genes of the pS88 plasmid were detected in all but two strains (one human and one calf STEC). The Shiga toxin subtypes stx1a (n = 3; one human, two calf), stx2a (n = 2) and stx2d (n = 6) were detected. All strains were multidrug resistant, two were extended-spectrum β-lactamase positive. Core genome MLST revealed that some human and calf E. coli differed by only 22 loci. Conclusions: The STEC/ExPEC O80:H2 pathotype was present in calves in Belgium as early as 1987, but human infections have been rare and mostly mild. The human STEC and bovine aEPEC cluster together and have the potential to be as virulent as the French isolates, as shown by their similar gene content. © 2018 European Society of Clinical Microbiology and Infectious Diseases [less ▲]

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See detailEnteropathogenic Escherichia coli O80:H2 in Young Calves with Diarrhea, Belgium
Thiry, Damien ULiege; Saulmont, Marc; Takaki, Shino et al

in Emerging Infectious Diseases (2017), 23(12),

Serogroup O80 was detected in 40% of 104 enteropathogenic Escherichia coli isolates from calves with diarrhea from 42 farms in Belgium during 2008‒2015. These isolates harbored the eae-ξ and fliCH2 genes ... [more ▼]

Serogroup O80 was detected in 40% of 104 enteropathogenic Escherichia coli isolates from calves with diarrhea from 42 farms in Belgium during 2008‒2015. These isolates harbored the eae-ξ and fliCH2 genes, similar to the O80 attaching-effacing Shigatoxigenic E. coli isolates found in humans in France. This strain might be emerging. [less ▲]

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See detailImplementation of the Galleria mellonella larvae model to evaluate phage therapy against Klebsiella pneumoniae
Thiry, Damien ULiege; Passet, V.; Danis-Wlodarczyk, K. et al

Conference (2017, October 20)

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See detailImplementation of the Galleria mellonella larvae model to evaluate phage therapy against Klebsiella pneumoniae
Thiry, Damien ULiege; Passet, V.; Danis-Wlodarczyk, K. et al

Poster (2017, October)

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