References of "Le Goff, Caroline"
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See detailEvaluation of Metformin impact on vitamin B12 status
VRANKEN, Laura ULiege; FONTAINE, Corentin ULiege; LUKAS, Pierre ULiege et al

Poster (2019, May)

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See detailComparison of cardiac biomarker dynamics in marathon, semimarathon and untrained runners: what is the impact on results interpretation?
Le Goff, Caroline ULiege; Lennartz, Lieselotte; VRANKEN, Laura ULiege et al

in Journal of Laboratory and Precision Medicine (2019), 4(6), 10

Background: Cardiac biomarkers elevations have been described after running exercise. Objective of our study was to check cardiac biomarker dynamics in well trained athletes and untrained middle aged ... [more ▼]

Background: Cardiac biomarkers elevations have been described after running exercise. Objective of our study was to check cardiac biomarker dynamics in well trained athletes and untrained middle aged apparently healthy men and to highlight the impact on the lab results interpretation in emergency department. Methods: Cardiac biomarkers for ischemic condition, cardiac stretch and fibrotic processes were tested in different type of runners before, directly after and 3 hours after running. Markers for inflammation, muscle disease and renal function were also measured. Results: Cardiac biomarker levels between groups were not statistically different in the pre-exercise samples for natriuretic peptides [B-type natriuretic peptide (BNP), N-terminal Pro BNP (NT-ProBNP)] and galectin-3 (Gal-3), only Troponin I levels were higher. Directly after exercise, all cardiac biomarker levels were higher compared to the baseline, Gal-3 and BNP levels decreased 3 hours after completion of the run.Troponin values continued to increase with highest levels 3 hours after exercise. Troponin T, NT-Pro BNP and Gal-3 also showed significant correlation to markers of inflammation, fibrosis and renal function. Conclusions: Exercises of different intensity can be associated with biochemical abnormalities and longterm consequences are unknown. In chest pain patients presenting to the emergency department possible impact of exercise on test results, especially Troponin, should be checked. [less ▲]

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See detailEstablishment of reference intervals for serum concentrations of androstanediol glucuronide by a newly developed LC-MS/MS method.
Fabregat-Cabello, Neus; PEETERS, Stéphanie ULiege; Yilmaz, Tugba ULiege et al

in Steroids (2019), 143

BACKGROUND: Androstanediol glucuronide is linked to a range of disorders of peripheral androgen formation and action, such as in hirsutism and acne. Nowadays its accurate quantification is still ... [more ▼]

BACKGROUND: Androstanediol glucuronide is linked to a range of disorders of peripheral androgen formation and action, such as in hirsutism and acne. Nowadays its accurate quantification is still challenging and there are just a few LC-MS/MS methods available. Besides, their reference intervals for normal European populations by LC-MS/MS, including prepubertal and pubertal children, have not been reported yet. METHODS: Validation of the proposed new methodology was performed at 3 levels in triplicate during 3 different days. Calibration curve concentration ranged from 0.1 to 25microg/L. For method comparison between ELISA and the newly developed LC-MS/MS method, 43 patient samples were tested. A reference interval study was performed with 264 healthy Belgian individuals (108 male and 156 female). RESULTS: Validation of the proposed LC-MS/MS method was satisfactorily achieved, with mean imprecision values lower than 7.4%, mean recoveries within 99-108% and a limit of quantification of 0.059microg/L. Compared to LC-MS/MS, ELISA showed a positive bias in serum samples, providing results 43% higher for the same sample. As a consequence, new reference intervals based on age and gender have been calculated. CONCLUSION: An easy, fast and straightforward LC-MS/MS method for the determination of androstanediol glucuronide has been developed and fully validated. Besides, reference interval for normal European populations, including prepubertal and pubertal children has been established for the first time. [less ▲]

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See detailMétabolites de la Vitamine D: intérêts du dosage et recommandations pour les sportifs
LE GOFF, Caroline ULiege

Conference (2018, October 30)

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See detailINTÉRÊT DU DOSAGE DES STÉROÏDES par LC-MS/MS
LE GOFF, Caroline ULiege

Conference (2018, October 18)

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See detailIntérêts du dosage des métabolites de la Vitamine D
LE GOFF, Caroline ULiege

Conference (2018, September 21)

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See detailLab Developed Tests for Steroids: The Journey from Immunoassay to Mass Spectrometry
LE GOFF, Caroline ULiege

Conference (2018, September 19)

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See detailCortisol everywhere!
Arias-Carnero, Benjamin; PEETERS, Stéphanie ULiege; DEVILLE, Marine ULiege et al

Poster (2018, September 12)

Our problem with a routine LC-MS/MS system is related to the presence of an isobaric interference of cortisol from an unknown source. After cleaning and checking the proper function of the LC system, we ... [more ▼]

Our problem with a routine LC-MS/MS system is related to the presence of an isobaric interference of cortisol from an unknown source. After cleaning and checking the proper function of the LC system, we performed an exhaustive study of all the possible contamination sources that can affect the method performance. Bovine Serum Albumin (BSA) seems the critical reactive that contained the highest amount of this interference, among other sources tested. Identity of this compound was obtained by QTOF analysis and resulted to be cortisol. [less ▲]

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See detailDevelopment and Validation of the Simultaneous Measurement of Estrone and 17β-Estradiol in Serum by LC-MS/MS for Clinical Laboratory Applications
Pitarch-Motellón, Jorge; Cavalier, Etienne ULiege; LE GOFF, Caroline ULiege et al

Poster (2018, September 12)

A straightforward analytical method for the determination of estrone (E1) and 17β-estradiol (E2) at ultra-low levels in serum samples by LC-MS/MS has been developed and validated. This method entails an ... [more ▼]

A straightforward analytical method for the determination of estrone (E1) and 17β-estradiol (E2) at ultra-low levels in serum samples by LC-MS/MS has been developed and validated. This method entails an extraction and derivatization with dansyl chloride followed by separation with a C18 column. A comparison of the developed LC-MS/MS method against our routine immunoassay shows a good correlation for E2 while an important negative bias is observed for E1 by RIA. The later comparison for E1 shows the need to switch from the current routine automated immunoassays to highly-sensitive LC-MS/MS quantifications in order to provide accurate and reliable clinical results, especially at very low levels. [less ▲]

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See detailEccentric training for tendon healing after acute lesion: a rat model
Kaux, Jean-François ULiege; Libertiaux, Vincent; Leprince, Pierre ULiege et al

in Towards healthy tendon ageing... (2018, September)

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See detailCARDIAC BIOMARKERS FLUCTUATION IN RUNNERS OF MARATHONS, SEMI-MARATHONS AND UNTRAINED RUNNERS
Le Goff, Caroline ULiege; VRANKEN, Laura ULiege; Kaux, Jean-François ULiege et al

in 23rd Annual Congress of the ECSS (2018, July)

INTRODUCTION: Regular exercise like running is one important part of the prevention program of cardiovascular disease. There are several studies on biomarker changes during marathons especially cardiac ... [more ▼]

INTRODUCTION: Regular exercise like running is one important part of the prevention program of cardiovascular disease. There are several studies on biomarker changes during marathons especially cardiac biomarkers have been studied and mild to moderate elevations have been described as a results of a running exercise Exact underlying mechanism for these biomarker elevations reflecting physiological or even pathobiological changes is unknown and less trained athletes might exhibit a higher risk compared to well trained. The aim of our study was to compare three cardiac biomarkers for ischemic condition , cardiac stretch and fibrotic processes were tested in different type of runners, trained marathon and semi-marathon runners and untrained runners before, directly after and 3 hours after the running exercise. METHODS: 23 marathon runners, 15 semi-marathon runners <44.1±8.4yo> and 17 healthy sedentary subjects < 37± 4.4 yo> were enrolled in our study. Blood samples were taken just before , just after and 3 hours after the race, centrifuged, aliquoted and stored frozen at -80°C before further analysis. The study was approved by the Ethical Committee of our University Hospital. The analyses were performed on the Abbott ARCHITECT i2000SR for the hs cTnI, BNP and Gal-3 and on the C8000 for hs-cTnT and NTproBNP according to the manufacturer’s instructions for use. RESULTS: In all 3 running groups there is an increase of cardiac biomarkers Troponin I, BNP, Galectin-3 and NT-ProBNP after completion of the physical exercise. Biomarkers increase is depending on the intensity and duration of the exercise and is higher in long distance marathon and semi-marathon runners compared to the control group with a 1 hour run. Cardiac biomarker levels between trained marathon and semi-marathon runners were not statistically different in the pre-exercise baseline samples for BNP, NT-Pro-BNP and Galectin-3. Compared to untrained runners only Troponin I levels were higher in baseline sample of marathon runners when compared to controls, cardiac Troponin T was less significant. CONCLUSION: In conclusion, the question whether running exercise of different intensity could be harmful to the heart has no simple answer. We could show that running exercise can be associated with biochemical abnormalities that may reflect adverse consequences on the heart like possible micro necrosis, oxidative stress, fibrosis and myocardial stretch. With exception of Troponin where levels continue to raise after end of running, NPs and Gal-3 levels normalized relatively fast after the exercise. The possible harmful effect of longer term cardiac consequences of repeated intensive sport acitvities still needs to be demonstrated. [less ▲]

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See detailRecertification of 25-hydroxyvitamin D standards by Isotope Pattern Deconvolution (IPD)
Fabregat Cabello, Neus ULiege; Pitarch-Motellón, Jorge; LE GOFF, Caroline ULiege et al

Scientific conference (2018, April 20)

Vitamin D (VTD) is an important prohormone widely known since its deficiency is directly related to development of rickets in children and osteoporosis in adults. Furthermore, recent studies have ... [more ▼]

Vitamin D (VTD) is an important prohormone widely known since its deficiency is directly related to development of rickets in children and osteoporosis in adults. Furthermore, recent studies have demonstrated that vitamin D has also an important role in non-skeletal conditions such as autoimmune diseases, cardiovascular diseases and cancer, among others. This vitamin can be found in two main forms: vitamin D2 and vitamin D3. The metabolism of both forms of vitamin D are subjected to a first hydroxylation in the liver to form 25-hydroxyvitamin D (25(OH)D) and then to a second one in the kidney to form 1,25-dihydroxyvitamin D (1,25(OH)2D), the active form of vitamin D. Nevertheless, the measurement of 25(OH)D in serum samples is preferred test for the assessment of vitamin D status over the 1,25(OH)2D. There are two main reasons for this choice: the longer lifetime (3 weeks versus 4 h) and its higher concentration levels (ng/mL versus pg/mL)[2]. Over the last years, a dramatic rise in vitamin D testing (as 25(OH)D) has been observed, due to two main reasons: the increased number of patients with VTD deficiency and the increase of the role of VTD as a biomarker related to several diseases. In this work we propose a recertification approach for 25(OH)D2/D3 solvent standards based on Isotope Pattern Deconvolution (IPD) using NIST SRM 2972 as reference material. This approach could help to meet the requirements for external standardization criteria using in-house calibration curves. [less ▲]

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See detailDealing with small sample volumes and analyte levels: the case of vitamin D metabolites in aqueous humor
Fabregat Cabello, Neus ULiege; LE GOFF, Caroline ULiege; Darimont, Pierre et al

Conference (2018, March 16)

Vitamin D is a very well known prohormone that plays a crucial role in the regulation of calcium and phosphate homeostasis, together with rickets and osteomalacia prevention. Recently a relationship ... [more ▼]

Vitamin D is a very well known prohormone that plays a crucial role in the regulation of calcium and phosphate homeostasis, together with rickets and osteomalacia prevention. Recently a relationship between the levels of vitamin D in serum and the presence of primary open angle glaucoma has also been demonstrated (Goncalves et al. 2015). A major risk factor in this affection is the increase of the intraocular pressure due to aqueous humor draining blockage. However, the screening of the status of vitamin D metabolites in the aqueous humor has not been performed yet. As a consequence our objective was to developed a method for the determination of the main metabolites of vitamin D (including 24,25(OH)2D2/D3 and 25(OH)D2/D3) in aqueous humor by LC-MS/MS. The main challenges derived from this method development are related to two main drawbacks: the limited sample volume and the low levels of analytes expected. On the one hand, since the aqueous humor is extracted during the cataract surgery, only a few microliters can be drawn, and this can be performed only once. Besides, an initial screening of the normal levels in samples has shown very low levels in patient samples (from low ng/mL to pg/mL). This leads to the worst case scenario: an extremely low absolute amount of analite available to be measured within the system. In order to overcome these problems, the proposed sample preparation is based on a simple liquid-liquid extraction followed by derivatization with the commercially available reagent Amplifex®. This has permitted to reach these extremely low levels with an accurate sample preparation. The proposed methodology is under validation process. [less ▲]

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See detailDosage des catécholamines et des métabolites par LC-MS/MS
LE GOFF, Caroline ULiege

Conference (2018, March 13)

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See detailLes biomarqueurs cardiaques: le tour de la question
LE GOFF, Caroline ULiege

Conference (2018, March 09)

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