References of "Lambert, Cécile"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailA systematic review of the secretome of skeletal muscle cells
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

Poster (2019, May 03)

This poster presents the preliminary results of a systematic review that summarized recent mass-spectrometry based proteomics discoveries of the secretome of skeletal muscle cells in response to disease ... [more ▼]

This poster presents the preliminary results of a systematic review that summarized recent mass-spectrometry based proteomics discoveries of the secretome of skeletal muscle cells in response to disease, exercise or metabolic stress. [less ▲]

Detailed reference viewed: 32 (7 ULiège)
Full Text
Peer Reviewed
See detailSécrétome des cellules musculaires squelettiques: revue systématique de la littérature
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

Conference (2019, April 04)

Présentation des résultats préliminaires d'une revue systématique en cours de rédaction portant sur le sécrétome des cellules musculaires squelettiques. L'étude du sécrétome de ces cellules pourrait ... [more ▼]

Présentation des résultats préliminaires d'une revue systématique en cours de rédaction portant sur le sécrétome des cellules musculaires squelettiques. L'étude du sécrétome de ces cellules pourrait permettre de comprendre les mécanismes liés au vieillissement mais également de permettre la découverte de biomarqueurs et de cibles thérapeutiques de certaines maladies [less ▲]

Detailed reference viewed: 44 (12 ULiège)
Full Text
Peer Reviewed
See detailSyndecan-4 is increased in osteoarthritic knee, but nor ip or shoulder, articular hypertrophic chondrocytes
Sanchez, Christelle ULiege; Lambert, Cécile ULiege; Dubuc, Jean-Emile et al

in Osteoarthritis and Cartilage (2019, April), 27(Suppl 1), 159-160

Detailed reference viewed: 12 (2 ULiège)
Full Text
Peer Reviewed
See detailThe secretome of skeletal muscle cells: A systematic review
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

Poster (2019, February 20)

This poster presents the preliminary results of a systematic review that summarized recent mass-spectrometry based proteomics discoveries of the secretome of skeletal muscle cells in response to disease ... [more ▼]

This poster presents the preliminary results of a systematic review that summarized recent mass-spectrometry based proteomics discoveries of the secretome of skeletal muscle cells in response to disease, exercise or metabolic stress. [less ▲]

Detailed reference viewed: 78 (25 ULiège)
Full Text
Peer Reviewed
See detailLe syndécan-4 est un marqueur de l’hypertrophie des chondrocytes du genou arthrosique
Sanchez, Christelle ULiege; Lambert, Cécile ULiege; Dubuc, JE et al

in Revue du Rhumatisme (2018, November), 85S

Detailed reference viewed: 8 (0 ULiège)
Full Text
Peer Reviewed
See detailGlycation marker glucosepane increases with the progression of osteoarthritis and correlates with morphological and functional changes of cartilage in vivo
Legrand, Catherine; Ahmed, U.; Anwar, A. et al

in Arthritis Research and Therapy (2018), 20(1), 131

Background: Changes of serum concentrations of glycated, oxidized, and nitrated amino acids and hydroxyproline and anticyclic citrullinated peptide antibody status combined by machine learning techniques ... [more ▼]

Background: Changes of serum concentrations of glycated, oxidized, and nitrated amino acids and hydroxyproline and anticyclic citrullinated peptide antibody status combined by machine learning techniques in algorithms have recently been found to provide improved diagnosis and typing of early-stage arthritis of the knee, including osteoarthritis (OA), in patients. The association of glycated, oxidized, and nitrated amino acids released from the joint with development and progression of knee OA is unknown. We studied this in an OA animal model as well as interleukin-1β-activated human chondrocytes in vitro and translated key findings to patients with OA. Methods: Sixty male 3-week-old Dunkin-Hartley guinea pigs were studied. Separate groups of 12 animals were killed at age 4, 12, 20, 28 and 36 weeks, and histological severity of knee OA was evaluated, and cartilage rheological properties were assessed. Human chondrocytes cultured in multilayers were treated for 10 days with interleukin-1β. Human patients with early and advanced OA and healthy controls were recruited, blood samples were collected, and serum or plasma was prepared. Serum, plasma, and culture medium were analyzed for glycated, oxidized, and nitrated amino acids. Results: Severity of OA increased progressively in guinea pigs with age. Glycated, oxidized, and nitrated amino acids were increased markedly at week 36, with glucosepane and dityrosine increasing progressively from weeks 20 and 28, respectively. Glucosepane correlated positively with OA histological severity (r = 0.58, p < 0.0001) and instantaneous modulus (r = 0.52-0.56; p < 0.0001), oxidation free adducts correlated positively with OA severity (p < 0.0009-0.0062), and hydroxyproline correlated positively with cartilage thickness (p < 0.0003-0.003). Interleukin-1β increased the release of glycated and nitrated amino acids from chondrocytes in vitro. In clinical translation, plasma glucosepane was increased 38% in early-stage OA (p < 0.05) and sixfold in patients with advanced OA (p < 0.001) compared with healthy controls. Conclusions: These studies further advance the prospective role of glycated, oxidized, and nitrated amino acids as serum biomarkers in diagnostic algorithms for early-stage detection of OA and other arthritic disease. Plasma glucosepane, reported here for the first time to our knowledge, may improve early-stage diagnosis and progression of clinical OA. © 2018 The Author(s). [less ▲]

Detailed reference viewed: 26 (3 ULiège)
Full Text
Peer Reviewed
See detailProteomic analysis of osteoblasts secretome provides new insights in mechanisms underlying osteoarthritis subchondral bone sclerosis
Sanchez, Christelle ULiege; Mazzucchelli, Gabriel ULiege; Comblain, Fanny et al

in Annals of the Rheumatic Diseases (2018, June), 77(supplement 2), 894

Detailed reference viewed: 37 (10 ULiège)
Full Text
Peer Reviewed
See detailTHE COLL2–1 PEPTIDE OF COLLAGEN TYPE II: A NEW ACTOR OF SYNOVITIS IN OSTEOARTHRITIS
Lambert, Cécile ULiege; Borderie, D; Rannou, F et al

in Annals of the Rheumatic Diseases (2018, June), 77(supplement 2), 890

Detailed reference viewed: 26 (6 ULiège)
Full Text
Peer Reviewed
See detailGLUCOSEPANE : A NEW BIOMARKER OF THE SEVERITY OF OSTEOARTHRITIS
Rabbani, N; Ahmed, U; Lambert, Cécile ULiege et al

in Annals of the Rheumatic Diseases (2018, June), 77(supplement 2), 181

Detailed reference viewed: 30 (1 ULiège)
Full Text
Peer Reviewed
See detailComparison of secretome from osteoblasts derived from sclerotic versus non-sclerotic subchondral bone in OA: A pilot study.
Sanchez, Christelle ULiege; Mazzucchelli, Gabriel ULiege; Lambert, Cécile ULiege et al

in PLoS ONE (2018), 13(3), 0194591

OBJECTIVE: Osteoarthritis (OA) is characterized by cartilage degradation but also by other joint tissues modifications like subchondral bone sclerosis. In this study, we used a proteomic approach to ... [more ▼]

OBJECTIVE: Osteoarthritis (OA) is characterized by cartilage degradation but also by other joint tissues modifications like subchondral bone sclerosis. In this study, we used a proteomic approach to compare secretome of osteoblast isolated from sclerotic (SC) or non sclerotic (NSC) area of OA subchondral bone. DESIGN: Secretome was analyzed using differential quantitative and relative label free analysis on nanoUPLC G2 HDMS system. mRNA of the more differentially secreted proteins were quantified by RT-PCR in cell culture from 5 other patients. Finally, osteomodulin and fibulin-3 sequences were quantified by western blot and immunoassays in serum and culture supernatants. RESULTS: 175 proteins were identified in NSC osteoblast secretome. Data are available via ProteomeXchange with identifier PXD008494. Compared to NSC osteoblast secretome, 12 proteins were significantly less secreted (Osteomodulin, IGFBP5, VCAM-1, IGF2, 78 kDa glucose-regulated protein, versican, calumenin, IGFBP2, thrombospondin-4, periostin, reticulocalbin 1 and osteonectin), and 13 proteins were significantly more secreted by SC osteoblasts (CHI3L1, fibulin-3, SERPINE2, IGFBP6, SH3BGRL3, SERPINE1, reticulocalbin3, alpha-2-HS-glycoprotein, TIMP-2, IGFBP3, TIMP-1, SERPINF1, CSF-1). Similar changes in osteomodulin, IGF2, SERPINE1, fibulin-3 and CHI3L1 mRNA levels were observed. ELISAs assays confirm the decrease by half of osteomodulin protein in SC osteoblasts supernatant compared to NSC and in OA patients serum compared to healthy subjects. Fibulin-3 epitopes Fib3-1, Fib3-2 and Fib3-3 were also increased in SC osteoblasts supernatant compared to NSC. CONCLUSIONS: We highlighted some proteins differentially secreted by the osteoblasts coming from OA subchondral bone sclerosis. These changes contribute to explain some features observed in OA subchondral bone, like the increase of bone remodeling or abnormalities in bone matrix mineralization. Among identified proteins, osteomodulin was found decreased and fibulin-3 increased in serum of OA patients. These findings suggest that osteomodulin and fibulin-3 fragments could be biomarkers to monitor early changes in subchondral bone metabolism in OA. [less ▲]

Detailed reference viewed: 30 (6 ULiège)
Full Text
Peer Reviewed
See detailStudy of the evolution of the osteoarthritis pathology and the mechanical properties of cartilage in a spontaneous osteoarthritis model in the Dunkin-Hartley guinea pigs.
Legrand, Catherine ULiege; Centonze, Prescilia ULiege; Comblain, Fanny ULiege et al

Poster (2017, April 27)

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes ... [more ▼]

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes. In parallel to these histological analyzes, we studied the mechanical properties of cartilage at different stages of disease progression in the Dunkin-Hartley guinea pigs. We also correlated the severity of histological lesions with the mechanical properties of cartilage. [less ▲]

Detailed reference viewed: 56 (5 ULiège)
Full Text
Peer Reviewed
See detailStudy of the evolution of the osteoarthritis pathology and the mechanical properties of cartilage in a spontaneous osteoarthritis model in the Dunkin-Hartley guinea pigs.
Legrand, Catherine ULiege; Centonze, Prescilia ULiege; Comblain, Fanny ULiege et al

in Osteoarthritis and Cartilage (2017, April), 25

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan con- tent, the integrity of the tidemark, the cellularity, and osteophytes ... [more ▼]

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan con- tent, the integrity of the tidemark, the cellularity, and osteophytes. In parallel to these histological analyzes, we studied the mechanical properties of cartilage at different stages of disease progression in the Dunkin-Hartley guinea pigs. We also correlated the severity of histo- logical lesions with the mechanical properties of cartilage. [less ▲]

Detailed reference viewed: 43 (6 ULiège)
Full Text
Peer Reviewed
See detailLe peptide Coll2-1 du collagène de type II : un nouvel acteur de la synovite dans l’arthrose
Lambert, Cécile ULiege; Borderie, Didier; Rannou, François et al

in Revue du Rhumatisme (2017), 84S

Detailed reference viewed: 26 (3 ULiège)
Full Text
Peer Reviewed
See detailLe glucosépane : un nouveau marqueur de la sévérité de l’arthrose
Lambert, Cécile ULiege; Legrand, Catherine; Ahmed, Usman et al

in Revue du Rhumatisme (2017), 84S

Detailed reference viewed: 22 (4 ULiège)
Full Text
Peer Reviewed
See detailReview of soluble biomarkers of osteoarthritis: lessons from animal model
Legrand, Catherine ULiege; Lambert, Cécile ULiege; Comblain, Fanny ULiege et al

in Cartilage (2017)

Osteoarthritis (OA) is one of the leading causes of disability within the adult population. Currently, its diagnosis is mainly based on clinical examination and standard radiography. To date, there is no ... [more ▼]

Osteoarthritis (OA) is one of the leading causes of disability within the adult population. Currently, its diagnosis is mainly based on clinical examination and standard radiography. To date, there is no way to detect the disease at a molecular level, before the appearance of structural changes and symptoms. So an attractive alternative for monitoring OA is the measurement of biochemical markers in blood, urine, or synovial fluid, which could reflect metabolic changes in joint tissue and therefore disease onset and progression. Animal models are relevant to investigate the early stage of OA and metabolic changes occurring in joint tissues. The goal of this narrative review is to summarize the scientific data available in the literature on soluble biomarkers in animal models of OA. [less ▲]

Detailed reference viewed: 32 (5 ULiège)
Full Text
Peer Reviewed
See detailStudy of the evolution of the osteoarthritis pathology and the mechanical properties of cartilage in a spontaneous osteoarthritis model in the Dunkin-Hartley guinea pigs.
Legrand, Catherine ULiege; Centonze, Prescilia ULiege; Comblain, Fanny ULiege et al

Poster (2016, November 16)

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes ... [more ▼]

In animal models, the severity of cartilage damage is assessed by histological scores evaluating the structure, the proteoglycan content, the integrity of the tidemark, the cellularity, and osteophytes. In parallel to these histological analyzes, we studied the mechanical properties of cartilage at different stages of disease progression in the Dunkin-Hartley guinea pigs. We also correlated the severity of histological lesions with the mechanical properties of cartilage. [less ▲]

Detailed reference viewed: 39 (4 ULiège)