References of "Kerckhofs, Greet"
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See detailDeveloping a predictive empirical model to optimize biomaterials characteristics for intra-oral bone regeneration
Sadeghian Dehkord, Ehsan ULiege; Kerckhofs, Greet; Lambert, France ULiege et al

Scientific conference (2018, September 11)

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See detailBone regeneration in healthy versus controlled diabetic conditions: Influence of leukocyte- and platelet-rich fibrin (L-PRF)ion
BACEVIC, Miljana ULiege; KERCKHOFS, Greet; ROGANOVIC, Jelena et al

Poster (2018, June)

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See detailSimultaneous three-dimensional visualization of mineralized and soft skeletal tissues by a novel microCT contrast agent with polyoxometalate structure.
Kerckhofs, Greet; Stegen, Steve; van Gastel, Nick et al

in Biomaterials (2018), 159

Biological tissues have a complex and heterogeneous 3D structure, which is only partially revealed by standard histomorphometry in 2D. We here present a novel chemical compound for contrast-enhanced ... [more ▼]

Biological tissues have a complex and heterogeneous 3D structure, which is only partially revealed by standard histomorphometry in 2D. We here present a novel chemical compound for contrast-enhanced microfocus computed tomography (CE-CT), a Hafnium-based Wells-Dawson polyoxometalate (Hf-POM), which allows simultaneous 3D visualization of mineralized and non-mineralized skeletal tissues, such as mineralized bone and bone marrow vasculature and adipocytes. We validated the novel contrast agent, which has a neutral pH in solution, by detailed comparison with (immuno)histology on murine long bones as blueprint, and showed that Hf-POM-based CE-CT can be used for virtual 3D histology. Furthermore, we quantified the 3D structure of the different skeletal tissues, as well as their spatial relation to each other, during aging and diet-induced obesity. We discovered, based on a single CE-CT dataset per sample, clear differences between the groups in bone structure, vascular network organization, characteristics of the adipose tissue and proximity of the different tissues to each other. These findings highlight the complementarity and added value of Hf-POM-based CE-CT compared to standard histomorphometry. As this novel technology provides a detailed 3D simultaneous representation of the structural organization of mineralized bone and bone marrow vasculature and adipose tissue, it will enable to improve insight in the interactions between these three tissues in several bone pathologies and to evaluate the in vivo performance of biomaterials for skeletal regeneration. [less ▲]

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See detailComputational modelling of local calcium ions release from calcium phosphate-based scaffolds
Manhas, Varun ULiege; Guyot, Yann; Kerckhofs, Greet et al

in Biomechanics and Modeling in Mechanobiology (2016)

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See detailInvestigating in vitro calcium dissolution using a mathematical model
Manhas, Varun ULiege; Guyot, Yann; Kerckhofs, Greet et al

Conference (2016, March)

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See detailMultifactorial Optimization of Contrast-Enhanced Nanofocus Computed Tomography for Quantitative Analysis of Neo-Tissue Formation in Tissue Engineering Constructs.
Sonnaert, Maarten; Kerckhofs, Greet; Papantoniou, Ioannis et al

in PLoS ONE (2015), 10(6), 0130227

To progress the fields of tissue engineering (TE) and regenerative medicine, development of quantitative methods for non-invasive three dimensional characterization of engineered constructs (i.e. cells ... [more ▼]

To progress the fields of tissue engineering (TE) and regenerative medicine, development of quantitative methods for non-invasive three dimensional characterization of engineered constructs (i.e. cells/tissue combined with scaffolds) becomes essential. In this study, we have defined the most optimal staining conditions for contrast-enhanced nanofocus computed tomography for three dimensional visualization and quantitative analysis of in vitro engineered neo-tissue (i.e. extracellular matrix containing cells) in perfusion bioreactor-developed Ti6Al4V constructs. A fractional factorial 'design of experiments' approach was used to elucidate the influence of the staining time and concentration of two contrast agents (Hexabrix and phosphotungstic acid) and the neo-tissue volume on the image contrast and dataset quality. Additionally, the neo-tissue shrinkage that was induced by phosphotungstic acid staining was quantified to determine the operating window within which this contrast agent can be accurately applied. For Hexabrix the staining concentration was the main parameter influencing image contrast and dataset quality. Using phosphotungstic acid the staining concentration had a significant influence on the image contrast while both staining concentration and neo-tissue volume had an influence on the dataset quality. The use of high concentrations of phosphotungstic acid did however introduce significant shrinkage of the neo-tissue indicating that, despite sub-optimal image contrast, low concentrations of this staining agent should be used to enable quantitative analysis. To conclude, design of experiments allowed us to define the most optimal staining conditions for contrast-enhanced nanofocus computed tomography to be used as a routine screening tool of neo-tissue formation in Ti6Al4V constructs, transforming it into a robust three dimensional quality control methodology. [less ▲]

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See detailInvestigating cell-carrier combinations for bone formation using a mathematical model
Manhas, Varun ULiege; Guyot, Yann ULiege; Chai, Yoke C et al

Poster (2014, November 28)

This study describes the development and implementation of a bio-regulatory model that focuses on the Ca2+ release from CaP scaffolds and aims to determine the local Ca2+ concentration of different ... [more ▼]

This study describes the development and implementation of a bio-regulatory model that focuses on the Ca2+ release from CaP scaffolds and aims to determine the local Ca2+ concentration of different clinical grade CaP scaffold types during bone formation. [less ▲]

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See detailMathematical modelling of bone formation based on local Ca2+ concentrations in calcium phosphate scaffolds
Manhas, Varun ULiege; Guyot, Yann; Chai, Yoke Chin et al

Poster (2014, September)

In tissue engineering applications, scaffolds act as carriers for cells and growth factors that restore the functionality of an organ either permanently or temporarily. Since their introduction, calcium ... [more ▼]

In tissue engineering applications, scaffolds act as carriers for cells and growth factors that restore the functionality of an organ either permanently or temporarily. Since their introduction, calcium phosphates (CaP) have proven to be an excellent bone substitute material, however, the complexity of the in vitro and in vivo behavior has not been elucidated completely. Computational models can help us to gain a comprehensive understanding of this complexity, allowing improvement of the design for optimal bone healing. For the development of a mathematical model accounting for the complexity of CaP driven bone formation, this study focuses on the Ca2+ release from CaP scaffolds and aims to determine the local Ca2+ concentration of different clinical grade CaP scaffold types during bone formation. [less ▲]

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