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See detailSaturated fatty acids induce NLRP3 activation in human macrophages through K+ efflux resulting from phospholipid saturation and Na, K-ATPase disruption
Gianfrancesco, Marco ULiege; Dehairs, Jonas; L'homme, Laurent et al

in BBA - Molecular and Cell Biology of Lipids (2019), 1864

NLRP3 inflammasome plays a key role in Western diet–induced systemic inflammation and was recently shown to mediate long-lasting trained immunity in myeloid cells. Saturated fatty acids (SFAs) are sterile ... [more ▼]

NLRP3 inflammasome plays a key role in Western diet–induced systemic inflammation and was recently shown to mediate long-lasting trained immunity in myeloid cells. Saturated fatty acids (SFAs) are sterile triggers able to induce the assembly of the NLRP3 inflammasome in macrophages, leading to IL-1β secretion while unsaturated ones (UFAs) prevent SFAs-mediated NLRP3 activation. Unlike previous studies using LPS-primed bone marrow derived macrophages, we do not see any ROS or IRE-1α involvement in SFAs-mediated NLRP3 activation in human monocytes-derived macrophages. Rather we show that SFAs need to enter the cells and to be activated into acyl-CoA to lead to NLRP3 activation in human macrophages. However, their β-oxidation is dispensable. Instead, they are channeled towards phospholipids but redirected towards lipid droplets containing triacylglycerol in the presence of UFAs. Lipidomic analyses and Laurdan fluorescence experiments demonstrate that SFAs induce a dramatic saturation of phosphatidylcholine (PC) correlated with a loss of membrane fluidity, both events inhibited by UFAs. The silencing of CCTα, the key enzyme in PC synthesis, prevents SFA-mediated NLRP3 activation, demonstrating the essential role of the de novo PC synthesis. This SFA-induced membrane remodeling promotes a disruption of the plasma membrane Na, K-ATPase, instigating a K+ efflux essential and sufficient for NLRP3 activation. This work opens novel therapeutic avenues to interfere with Western diet-associated diseases such as those targeting the glycerolipid pathway. [less ▲]

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See detailAntiplasmodial Diterpenoids from Psiadia arguta.
Mahadeo, Keshika; Herbette, Gaetan; Grondin, Isabelle et al

in Journal of natural products (2019)

An ethyl acetate extract of Psiadia arguta leaves showed in vitro antiplasmodial activity against Plasmodium falciparum with IC50 values of 12.3 +/- 2.4 mug/mL (3D7 strain) and 13.5 +/- 3.4 mug/mL (W2 ... [more ▼]

An ethyl acetate extract of Psiadia arguta leaves showed in vitro antiplasmodial activity against Plasmodium falciparum with IC50 values of 12.3 +/- 2.4 mug/mL (3D7 strain) and 13.5 +/- 3.4 mug/mL (W2 strain). Phytochemical investigation led to the isolation and characterization of 16 compounds including four new diterpenoids: labdan-8alpha-ol-15-yl-(formate) (1), labdan-8alpha-ol-15-yl-(2-methylbutanoate) (2), labdan-8alpha-ol-15-yl-(3-methylpentanoate) (3), and labdan-8alpha-ol-15-yl-(labdanolate) (4). The latter compounds were characterized by spectroscopic methods (1D and 2D NMR, HRMS, and IR). The in vitro antiplasmodial activities of all compounds were evaluated. The known compounds labdan-13( E)-en-8alpha-ol-15-yl acetate (5), labdan-8alpha-ol-15-yl acetate (6), 13- epi-sclareol (7), labdan-13( E)-ene-8alpha,15-diol (8), and (8 R,13 S)-labdane-8alpha,15-diol (9) exhibited antiplasmodial effects, with IC50 values of 29.1, 33.2, 35.0, 36.6, and 22.2 muM, respectively. [less ▲]

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See detailPreparation of ellagic acid derivatives through a total synthesis approach to improve bioavailability.
Degotte, Gilles ULiege; Halleux, Annabelle; Hans, Aurore et al

Poster (2018, December)

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See detailAntimalarial and antitrypanosomal activity of strychnogucine B, a bisindole alkaloid from Strychnos icaja
Beaufay, Claire; Ledoux, Allison ULiege; Frederich, Michel ULiege et al

Poster (2018, July)

Strychnogucine B, a bisindole alkaloid isolated from Strychnos icaja (Loganiaceae), showed promising in vitro antiplasmodial properties (IC50 < 1μM) in previous studies [1, 2]. In order to obtain ... [more ▼]

Strychnogucine B, a bisindole alkaloid isolated from Strychnos icaja (Loganiaceae), showed promising in vitro antiplasmodial properties (IC50 < 1μM) in previous studies [1, 2]. In order to obtain sufficient quantities to further investigate its antiprotozoal activity, this compound was synthesized in four steps from (-)-strychnine [3]. The in vivo antimalarial activity of this semi-synthetic strychnogucine B (30 mg/kg/d, i.p) was determined in a Plasmodium berghei murine model using the Peters’ 4-day suppressive test. This alkaloid confirmed a promising antimalarial activity in vivo by suppressing the parasitaemia by almost 36 percent on day 5 and 60 percent on day 7 compared to vehicle treated mice. In addition to this interesting antiplasmodial potential, it also showed a moderate in vitro anti-trypanosomal activity (IC50 = 2.7μM) but no in vivo activity in an acute Trypanosoma brucei model. It was also inactive in vitro on Leishmania mexicana promastigotes. This study highlights the selective antiplasmodial efficacy of strychnogucine B and warrants further investigations to assess the potential of this alkaloid as new antimalarial lead compound. [less ▲]

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See detailAntiplasmodial activity of polyphenolic derivatives.
Degotte, Gilles ULiege; Alson, S.G.; Hans, Aurore ULiege et al

Poster (2018, June 12)

Despite the progress in the struggle against malaria, this parasitic disease remains a major public health problem with 216 million cases in 2016. The last advance was the development of artemisinin and ... [more ▼]

Despite the progress in the struggle against malaria, this parasitic disease remains a major public health problem with 216 million cases in 2016. The last advance was the development of artemisinin and its derivatives, near 15 years ago. These compounds are employed with success in combination with other antimalarial drugs and are now the recommended treatment by the World Health Organization. However, resistance of Plasmodium to these molecules appears and spreads over in Asia and Africa. Thus, the design of new antiplasmodial derivatives is imperative to hope the eradication of this infection. Polyphenolics compounds are well known to have multiple pharmacological activities such as antioxidant2, antitumor3, antimicrobial4 and antiplasmodial5. Regarding this last effect, the screening of caffeic acid (1) and its derivatives was performed in vitro and in vivo. These evaluations permitted to select ethyl caffeate (2) as the most potent derivatives against a 3D7 strain in culture. More interestingly, this ester was found active in mice with a stage specificity on the young trophozoites. The major interest of this molecule is that caffeic acid is widely distributed in plants and is considered non-toxic. Considering the results obtained with this first series of polyphenolic analogs and based on the known antimalarial activity of ellagic acid (3) in vitro and in vivo6, we currently investigate this widely distributed polyphenol as a scaffold for further pharmacomodulation. The new structures will be screened to determine their antiplasmodial effect. [less ▲]

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See detailCapillary electrophoresis, high-performance liquid chromatography, and thin-layer chromatography analyses of phenolic compounds from rapeseed plants and evaluation of their antioxidant activity.
Huang, Yang; Jansen, Olivia ULiege; Frederich, Michel ULiege et al

in Journal of Separation Science (2018)

Rapeseed plants, known for oil production, are also known to contain phenolic compounds such as phenolic acids and flavonoids, with potential antioxidant and anticancer activities. The separation and ... [more ▼]

Rapeseed plants, known for oil production, are also known to contain phenolic compounds such as phenolic acids and flavonoids, with potential antioxidant and anticancer activities. The separation and identification of 11 phenolic acids in rapeseed extracts (including leaves, flowers, Chinese seeds, Belgian seeds, and cake) by capillary electrophoresis were investigated. The results were compared with those obtained with high-performance liquid chromatography and thin-layer chromatography and showed that the capillary electrophoresis technique offers several advantages for the identification of phenolic compounds in various rapeseed extracts. The antioxidant activity of rapeseed extracts and reference compounds was evaluated using four different approaches, namely, 2,2'-azinobis- (3-ethylbenzohiazoline-6-sulfonic acid assay, free radical 2,2-diphenyl-1-picrylhydrazyl assay, electron paramagnetic resonance spectroscopy and the measurement of the total polyphenol content. The contents of total polyphenols in the tested extracts were ranging between 5.4 and 21.1% m/m and ranked as follows: Chinese seeds > Belgian seeds > Flowers > Cake > Leaves. [less ▲]

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See detailIn-vitro and in-vivo antimalarial activity of caffeic acid and some of its derivatives.
Alson, Sylvain G.; Jansen, Olivia ULiege; Cieckiewicz, Ewa ULiege et al

in Journal of Pharmacy and Pharmacology (2018), 70(10), 1349-1356

OBJECTIVES: To explore the in-vitro and in-vivo antimalarial potential of caffeic acid and derivatives. METHODS: Two common phenolic acids (caffeic acid and chlorogenic acid) were evaluated for in-vitro ... [more ▼]

OBJECTIVES: To explore the in-vitro and in-vivo antimalarial potential of caffeic acid and derivatives. METHODS: Two common phenolic acids (caffeic acid and chlorogenic acid) were evaluated for in-vitro and in-vivo antiplasmodial activity in comparison with some semi-synthetic derivatives that were synthesized. An in-vitro assay based on plasmodial lactate dehydrogenase activity, and the classical in-vivo 5-day suppressive test from Peters on an artemisinin-resistant Plasmodium berghei strain was used. Parasitic stage sensitivity to ethyl caffeate was determined in this work. KEY FINDINGS: Phenolic acid esters derivatives showed better antiplasmodial activity than corresponding phenolic acids. The derivative with the highest in-vitro activity being caffeic acid ethyl ester, exhibiting an IC50 = 21.9 +/- 9.4 mum. Ethyl caffeate and methyl caffeate were then evaluated for antimalarial activity in vivo and ethyl caffeate showed a growth inhibition of 55% at 100 mg/kg. Finally, it seems that ethyl caffeate blocks the growth of young parasitic forms. CONCLUSIONS: Our study provides evidence for an antimalarial potential of caffeic acid derivatives which are common in several medicinal plants traditionally used against malaria. It also demonstrates the possibility to use such derivatives in the treatment of malaria. [less ▲]

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See detailAntiplasmodial, Anti-chikungunya virus and Antioxidant Activities of 64 endemic Plants from the Mascarene Islands.
Ledoux, Allison ULiege; Cao, Martine ULiege; Jansen, Olivia ULiege et al

in International Journal of Antimicrobial Agents (2018), 52(5), 622-628

Vector-borne diseases cause more than 1 million deaths annually. The research of new medicines is urgent, especially since there is currently no specific treatment. In this study, the authors have ... [more ▼]

Vector-borne diseases cause more than 1 million deaths annually. The research of new medicines is urgent, especially since there is currently no specific treatment. In this study, the authors have selected 64 endemic plants from the Mascarene Islands based on their endemism, their medicinal use and their registration in the French Pharmacopeia to evaluate the antiplasmodial, anti-chikungunya and antioxidant activities. The list of these 64 plants including their local name, population, data of collection and voucher number are available in the Supporting Information section. 40 active extracts have been identified from the 38 species: 22 responded positively to the antiplasmodial activity, 8 to the anti-chikungunya activity and 8 to the antioxidant activity. Six plants demonstrated high antiplasmodial activity (IC50 < 15 microg/mL): Casearia coriaceae, Monimia rotundifolia, Poupartia borbonica, Psiadia retusa, Vernonia fimbrillifera, and Zanthoxylum heterophyllum; and five showed high anti-chikungunya activity (IC50 < 20 microg/mL): Aphloia theiformis, Stillingia lineata, Croton mauritianus, Indigofera ammoxylum, and Securinega durissima. Eight plants displayed an important antioxidant activity, with values of ABTS, FRAP or ORAC > 2000 microM of Trolox equivalent per mg/mL of extract: Bertiera borbonica, Erythroxylon laurifolium, Erythroxylon sideroxyloides, Indigofera ammoxylum, Poupartia borbonica, Scolopia heterophylla, Sophora denudata, and Terminalia bentzoe. Some data obtained tend to corroborate with the reported traditional use of the plant, such as Zanthoxylum heterophyllum (antiplasmodial), Aphloia theiformis (anti-chikungunya), and Erythroxylon laurifolium (antioxidant). [less ▲]

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See detailIn vivo antimalarial and antitrypanosomal activity of strychnogucine B, a bisindole alkaloid from Strychnos icaja.
Beaufays, Claire; Ledoux, Allison ULiege; Jansen, Olivia ULiege et al

in Planta Medica (2018), 84

Strychnogucine B is a bisindole alkaloid previously isolated from Strychnos icaja that possesses promising in vitro antiplasmodial properties. This compound was synthesized in four steps from (− ... [more ▼]

Strychnogucine B is a bisindole alkaloid previously isolated from Strychnos icaja that possesses promising in vitro antiplasmodial properties. This compound was synthesized in four steps from (−)-strychnine. As no acute toxicity was observed at the highest tested cumulative dose of 60 mg/kg, its in vivo antimalarial activity was determined intraperitoneally at 30mg/kg/d in a Plasmodium berghei murine model. In the Petersʼs 4-d suppressive test, this alkaloid suppressed the parasitaemia by almost 36% on day 5 and 60% on day 7 compared to vehicle-treated mice. In addition to this interesting antimalarial activity, it showed moderate in vitro antitrypanosomal activity but no in vivo activity in an acute Trypanosoma brucei model. It was also inactive in vitro on Leishmania mexicana promastigotes. This highlights its selective antimalarial efficacy and leads to further investigation to assess its potential as new antimalarial lead compound. [less ▲]

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See detailProcessus d’intégration de la Médecine traditionnelle dans le système national de soins de la République Démocratique du Congo : Cas de trois zones de santé de Kinshasa
Mavungu Landu, Don Jethro ULiege; Mbuyi Kaluka Tshibanda, Jacques; Dasnoy, Christine ULiege et al

Conference (2017, November 10)

PROCESSUS D’INTEGRATION DE LA MEDECINE TRADITIONNELLE DANS LE SYSTEME NATIONAL DE SOINS DE LA REPUBLIQUE DEMOCRATIQUE DU CONGO : Cas de trois zones de santé de Kinshasa La médecine traditionnelle est ... [more ▼]

PROCESSUS D’INTEGRATION DE LA MEDECINE TRADITIONNELLE DANS LE SYSTEME NATIONAL DE SOINS DE LA REPUBLIQUE DEMOCRATIQUE DU CONGO : Cas de trois zones de santé de Kinshasa La médecine traditionnelle est reconnue comme un mode d’administration des soins de santé en RDC à travers les textes législatives et réglementaires suivants : la constitution de la République, la Loi cadre de la Santé et les arrêtés Ministériels du Ministre de la Santé Publique le premier portant organisation de l’exercice de la profession de praticien de la médecine traditionnelle et le deuxième portant création d’un Programme National de Promotion de la Médecine Traditionnelle et des Plantes Médicinales( PNMT-PM) dont l’une des missions est d’intégrer la médecine traditionnelle dans le système national des soins de santé. Elaboration de la réglementation sur la pratique de la médecine traditionnelle et de produits finis, des outils de collecte des données de la médecine traditionnelle et de la Pharmacopée traditionnelle , la mise en place des structures professionnelles de tradipraticiens et organisation du recensement des tradipraticiens de la ville de Kinshasa ainsi que la stratégie de l’OMS/Afro 2013-2023 qui recommande aux états membres d’ intégrer dans le système de soins les praticiens, les pratiques et les produits de la médecine traditionnelle pour lesquels existent des preuves d’ efficacité ,d’innocuité et de qualité ainsi que de produire ces preuves quand elles font défaut, la raison de ce projet pilote d’intégration de la médecine traditionnelle dans 3 Zones de santé (ZS) de Kinshasa à savoir KASA VUBU, MASINA 1 et SELEMBAO. Ce projet crée un cadre de collaboration entre l’équipe cadre de la Zone de santé et les tradipraticiens exerçant dans cette zone de santé(ZS). Chaque mois, le PNMT-PM avec la ZS collectent les informations relatives au travail des Tradipraticiens œuvrant dans la ZS (le nombre total des patients reçus ; le nombre des patients reçus par pathologie ; la durée du traitement par pathologie ; les recettes utilisées par pathologie ; le nombre de cas de réussite et d’échec par pathologie ; les attitudes du tradipraticien face au cas d’échec ; etc.). Ces informations permettront d’identifier et de sélectionner les meilleurs tradipraticiens, pratiques et produits à fin d’initier les études préalables pour l’intégration. En effet, de 2014-2015, la collecte des donnés était assurée par les animateurs communautaires au niveau de la ZS mais l’évaluation faite après, nous nous sommes rendus compte de l’insuffisance des données. D’où, en 2016 nous avons associé les relais communautaires qui collectent les données au niveau des aires de santé (un relais par aire de santé). Ce projet pilote nous a permis de tester et de valider les outils de collecte des données ainsi que d’intégrer dans le processus les 32 ZS restantes de la ville de Kinshasa en 2018. Ainsi, nous pensons que dans ce contexte, l’intégration sera possible et va permettre l’augmentation de la couverture sanitaire à travers la collaboration, la communication, l’harmonisation, l’établissement de partenariats entre la médecine traditionnelle et la médecine conventionnelle. [less ▲]

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See detailImplication du remodelage de la membrane plasmique par les acides gras dans la régulation de l’activité de l’inflammasome NLRP3 dans des macrophages humains
Gianfrancesco, Marco ULiege; Dehairs, Jonas; Bloch, Katarzyna et al

in Nutrition Clinique et Metabolisme (2017), 31(3), 255

Introduction et but de l’étude L’inflammation chronique et l’activation du système immunitaire sont impliquées dans le développement du diabète de type 2 chez les patients obèses. Des macrophages sont ... [more ▼]

Introduction et but de l’étude L’inflammation chronique et l’activation du système immunitaire sont impliquées dans le développement du diabète de type 2 chez les patients obèses. Des macrophages sont recrutés dans le tissu adipeux de ces patients et sécrètent des cytokines pro-inflammatoires dont l’IL-1β impliquée dans la résistance à l’insuline. Il a été montré que l’inflammasome NLRP3, requis pour la maturation de l’IL-1β, conduit au développement de l’inflammation induite par l’obésité et de la résistance à l’insuline. Contrairement à l’oléate (C18 :1), le stéarate (C18 :0), second acide gras saturé le plus abondant, conduit à l’activation de l’inflammasome NLRP3 dans des macrophages humains. L’addition de C18 :1 au C18 :0 empêche cette activation. Cependant, les mécanismes moléculaires sont inconnus. Matériel et méthodes Des analyses lipidomiques ont été réalisées sur des cellules THP-1 différenciées au PMA après 8h de traitement avec la BSA (témoin), le C18 :0 seul ou associé avec le C18 :1 pour étudier la composition en phospholipides cellulaires. La fluidité membranaire et la formation de gouttelettes lipidiques ont été observées par microscopie confocale avec les sondes fluorescentes Laurdan et HCS LipidTOX. Du C18 :0 radiomarqué a été utilisé pour suivre son incorporation dans les différentes classes lipidiques séparées par chromatographie sur couche mince. Résultats 0,0002) et PE (33,24 % contre 39,97 % des PEs totales ; p=0,0127). Conclusion L’augmentation de la saturation des phospholipides en présence de C18 :0 induit la rigidification de la membrane plasmique et pourrait mener à l’activation de l’inflammasome NLRP3. L’effet protecteur du C18 :1 est conduit par une réorientation de l’incorporation du C18 :0 des phospholipides vers les gouttelettes lipidiques, combiné à une fluidification membranaire due à l’augmentation des phospholipides insaturés. [less ▲]

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See detailAntimalarial Activities of Alkyl Cyclohexenone Derivatives Isolated from the Leaves of Poupartia borbonica
Ledoux, Allison ULiege; St-Gelais, Alexis; Cieckiewicz, Ewa ULiege et al

in Journal of Natural Products (2017), 80(6), 1750-1757

Bioactivity-guided fractionation of the ethyl acetate extract of the leaves of Poupartia borbonica led to the isolation of three new alkyl cyclohexenone derivatives 1−3, and named Poupartone A−C. The ... [more ▼]

Bioactivity-guided fractionation of the ethyl acetate extract of the leaves of Poupartia borbonica led to the isolation of three new alkyl cyclohexenone derivatives 1−3, and named Poupartone A−C. The structures of the new compounds were elucidated by 1D and 2D NMR spectroscopic data analysis and MS, whereas calculated and experimental ECD spectra were used to define the absolute configurations. These compounds were active against 3D7 and W2 Plasmodium falciparum strains with IC50 values between 0.55 and 1.81 μM. In vitro cytotoxicity against WI38 human fibroblasts and the human cervical cancer cell line HeLa (WST-1 assay) showed that these compounds were also cytotoxic, but no hemolytic activity was observed for the extract and pure compounds. An in vivo antimalarial assay was performed on the major cyclohexenone using P. berghei-infected mice at a dose of 15 mg/kg/day ip. The assay revealed growth inhibition of 59.1 and 69.5% at days 5 and 7 postinfection, respectively, although some toxicity was observed. Zebrafish larvae were used as a model to determine the type of toxicity, and the results showed cardiac toxicity. The methanol extract was also studied, and it displayed moderate antiplasmodial properties in vitro. This extract contained the known flavonoids, quercetin, 3′-O-hydroxysulfonylquercetin, quercitrin, and isoquercitrin as well as ellagic acid, which showed high to low activity against the 3D7 P. falciparum strain. [less ▲]

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See detailAntiplasmodial activity of Mezoneuron benthamianum leaves and identification of its active constituents
Jansen, Olivia ULiege; Tchinda, Alembert T.; Loua, Jean et al

in Journal of Ethnopharmacology (2017), 203(March), 20--26

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See detailAntiplasmodial activity of Heinsia crinita (Rubiaceae) and identification of new iridoids.
Tshisekedi Tshibangu, P.; Mutwale Kapepula, P.; Kabongo Kapinga, M. J. et al

in Journal of Ethnopharmacology (2017), 196

ETHNOPHARMACOLOGICAL RELEVANCE: Heinsia crinita is used in traditional medicine for the treatment of febrile illness and erectile dysfunction. Its stem bark powder is found in some peripheral markets in ... [more ▼]

ETHNOPHARMACOLOGICAL RELEVANCE: Heinsia crinita is used in traditional medicine for the treatment of febrile illness and erectile dysfunction. Its stem bark powder is found in some peripheral markets in the Democratic Republic of the Congo (DRC) as a remedy against malaria. Investigations were conducted on crude extracts of leaves, fruits and stem barks in view to validate their use and to determine which plant part possesses the best antiplasmodial properties. MATERIALS AND METHODS: Different plant parts were extracted with methanol, ethanol and dichloromethane. Based on the preliminary assays, the dichloromethane extract of the stem bark was subjected to fractionation using preparative HPLC system and column chromatography. This step led to the isolation of two new iridoids which had their structures elucidated by NMR, UV, MS and FT-IR spectroscopic techniques. Extracts and pure compounds were tested in vitro against the 3D7 strain of Plasmodium falciparum. The inhibition of the parasite growth was evaluated in vitro by colorimetric method (p-LDH assay) and their cytotoxicity evaluated in vitro against the human non-cancer fibroblast cell line (WI38) through WST1 assay. The in vivo antiplasmodial activity was assessed by the inhibition of Plasmodium berghei growth in infected mice treated with the ethanol extract of H. crinita stem bark at the concentrations of 200 and 300mg/Kg/day per os, using a protocol based on the 4-d suppressive test of Peters and compared to a non-treated negative control group of mice (growth =100%). Finally the antioxidant activity of the same extract was evaluated using ABTS, DPPH and cell-based assays. RESULTS: A moderate in vitro antiplasmodial activity was observed for the dichloromethane extract of the stem bark of H. crinita (IC50 =29.2+/-1.39microg/mL) and for the two new iridoids, lamalbide 6, 7, 8- triacetate (IC50 =16.39+/-0.43microg/mL) as well as for its aglycone lamiridosin 6, 7, 8-triacetate (IC50 =0.44.56+/-1.12microg/mL). The ethanolic stem bark extract (200 and 300mg/kg/day, oral route) showed a moderate in vivo antimalarial activity in Plasmodium berghei-infected mice with 27.84+/-2.75% and 48.54+/-3.76% of inhibition of the parasite growth, respectively (p<0.01).). This extract displayed high cellular antioxidant activity using dichlorofluorescein-diacetate (DCFDA) on HL-60 monocytes. These crude extracts and pure compounds tested at the higher concentration of 100microg/mL did not show any cytotoxicity against WI38 cells. CONCLUSIONS: The results showed that H. crinita extracts possess antimalarial activity and contain some unusual iridoids with moderate antiplasmodial activity, therefore justifying to some extent its traditional use by the local population in DRC for this purpose. This is the first report of the isolation and antiplasmodial activity of these two new iridoids. [less ▲]

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See detailValorization of Seeds from Some Field Border Flowering Seeds
Paul, Aman ULiege; Danthine, Sabine ULiege; Mutwale Kapepula, Paulin ULiege et al

Poster (2016, September 20)

Flowering strips are now being increasingly cultivated along the fields to improve biodiversity. However after serving for the desired function, these plants have no utilization besides animal feed. It ... [more ▼]

Flowering strips are now being increasingly cultivated along the fields to improve biodiversity. However after serving for the desired function, these plants have no utilization besides animal feed. It could be really interesting to valorize some commonly grown plant in these strips to render food or health promoting compounds. With this objective in mind the seeds of Achillea millefolium, Anthriscus sylvestris and Prunella vulgaris were investigated for lipids, proteins and phenolic content. Further the lipids were analyzed for fatty acid profile using gas chromatography and the phenolic compounds in the methanolic extract of defatted seeds were identified using HPLC-DAD. The antiradical activity of the methanolic extracts obtained from defatted seeds was investigated using DPPH and ABTS assays. The anti-inflammatory potential of these seed extracts was evaluated on the reactive oxygen species (ROS) production by stimulated neutrophils and on the specific activity of myeloperoxidase (MPO), a pro-oxidant enzyme marker of inflammation. Seeds from all three plants were analyzed with interesting levels of lipids, proteins and phenolic content. Linoleic acid, oleic acid and alpha-linolenic acid were the major fatty acids analyzed in A. millefolium, A. sylvestris and P. vulgaris respectively. On the other hand different phenolic acid formed the major phenolic constituents. Seed extracts displayed high ABTS and DPPH radical-scavenging activities in a dose dependent manner. Also a strong dose dependent anti-inflammatory activity of all three extracts was observed against ROS production by neutrophils and MPO activity. Results indicate that these seed show a great potential to render lipids which could be utilized as human food, further the defatted seeds could be directly included in human diet due to interesting levels of proteins and anti-inflammation ability. [less ▲]

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