References of "Henrotin, Yves"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailReduction of Matrix Metallopeptidase 13 and Promotion of Chondrogenesis by Zeel T in Primary Human Osteoarthritic Chondrocytes
Sanchez, Christelle ULiege; Hemmer, Kathrin; Krömmelbein, Natascha et al

in Frontiers in Pharmacology (2021), 12

Objectives: Zeel T (Ze14) is a multicomponent medicinal product. Initial preclinical data suggested a preventive effect on cartilage degradation. Clinical observational studies demonstrated that Ze14 ... [more ▼]

Objectives: Zeel T (Ze14) is a multicomponent medicinal product. Initial preclinical data suggested a preventive effect on cartilage degradation. Clinical observational studies demonstrated that Ze14 reduced symptoms of osteoarthritis (OA), including stiffness and pain. This study aimed to explore these effects further to better understand the mode of action of Ze14 on human OA chondrocytes in vitro. Methods: Primary chondrocytes were obtained from the knees of 19 OA patients and cultured either as monolayers or in alginate beads. The cultures were treated with 20% or 10% (v/v) Ze14 or placebo. For RNA-seq, reads were generated with Illumina NextSeq5000 sequencer and aligned to the human reference genome (UCSC hg19). Differential expression analysis between Ze14 and placebo was performed in R using the DESeq2 package. Protein quantification by ELISA was performed on selected genes from the culture medium and/or the cellular fractions of primary human OA chondrocyte cultures. Results: In monolayer cultures, Ze14 20% (v/v) significantly modified the expression of 13 genes in OA chondrocytes by at least 10% with an adjusted p-value < 0.05: EGR1, FOS, NR4A1, DUSP1, ZFP36, ZFP36L1, NFKBIZ, and CCN1 were upregulated and ATF7IP, TXNIP, DEPP1, CLEC3A, and MMP13 were downregulated after 24 h Ze14 treatment. Ze14 significantly increased (mean 2.3-fold after 24 h, p = 0.0444 and 72 h, p = 0.0239) the CCN1 protein production in human OA chondrocytes. After 72 h, Ze14 significantly increased type II collagen pro-peptide production by mean 27% (p = 0.0147). For both time points CCN1 production by OA chondrocytes was correlated with aggrecan (r = 0.66, p = 0.0004) and type II collagen pro-peptide (r = 0.64, p = 0.0008) production. In alginate beads cultures, pro-MMP-13 was decreased by Ze14 from day 7–14 (from −16 to −25%, p < 0.05) and from day 17–21 (−22%, p = 0.0331) in comparison to controls. Conclusion: Ze14 significantly modified the expression of DUSP1, DEPP1, ZFP36/ZFP36L1, and CLEC3A, which may reduce MMP13 expression and activation. Protein analysis confirmed that Ze14 significantly reduced the production of pro-MMP-13. As MMP-13 is involved in type II collagen degradation, Ze14 may limit cartilage degradation. Ze14 also promoted extracellular matrix formation arguably through CCN1 production, a growth factor well correlated with type II collagen and aggrecan production. [less ▲]

Detailed reference viewed: 16 (1 ULiège)
Full Text
Peer Reviewed
See detailMise au point d’un modèle de culture original de cellules musculaires squelettiques à partir de biopsies musculaires humaines
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

in Revue du Rhumatisme (2020, December), Volume 87(Supplément 1),

The objective of this work was to develop a culture model of primary skeletal muscle cells obtained from human muscle biopsies.

Detailed reference viewed: 27 (2 ULiège)
Full Text
Peer Reviewed
See detailAn oleuropein-based dietary supplement improves joint functionality in older people with hugh knee joint pain
Horcajada, Marie-Noelle; Beaumont, M; Sauvageot, N et al

in Annals of the Rheumatic Diseases (2020, June), 79(Suppl 1), 930

Detailed reference viewed: 12 (0 ULiège)
Full Text
Peer Reviewed
See detailBoswellia serrata extract and curcumin increase GDF15 production by human primary osteoarthritis chondrocytes: a new mechanism of action
Sanchez, Christelle ULiege; Zappia, Jérémie ULiege; Dierckxsens, Yvan et al

in Annals of the Rheumatic Diseases (2020, June), 79(Suppl 1), 1336-1337

Detailed reference viewed: 21 (2 ULiège)
Full Text
Peer Reviewed
See detailA multicomponent medication promotes chondrogenesis and reduces MMP-13 in primary articular chondrocytes from knee osteoarthritis patients in vitro
Sanchez, Christelle ULiege; Hemmer, Kathrin; Kroemmelbein, Natascha et al

in Annals of the Rheumatic Diseases (2020, June), 79(Suppl 1), 1336

Detailed reference viewed: 20 (1 ULiège)
Full Text
See detailLes douleurs de la gonarthrose. Comment les prendre en charge? Que dire à vos patients ?
Henrotin, Yves ULiege; Mathy, Céline

in Rhumatos (2020), 17(153), 159-166

La douleur est le principal symptôme qui conduit le patient arthrosique à consulter. Elle est généralement chronique et non linéaire. Sa prise en charge est essentiellement non pharmacologique et ... [more ▼]

La douleur est le principal symptôme qui conduit le patient arthrosique à consulter. Elle est généralement chronique et non linéaire. Sa prise en charge est essentiellement non pharmacologique et multidisciplinaire. Elle doit comprendre de l’information, de l’éducation au patient, une perte de poids si nécessaire, de l’activité physique (y compris des exercices) et une approche cognitivo-comportementale. [less ▲]

Detailed reference viewed: 59 (5 ULiège)
Full Text
Peer Reviewed
See detailCartilage biomarkers S-COLL2-1 and S-COLL2-1NO2 are helpful in identifying knee osteoarthritis patients at risk of disease worsening
Henrotin, Yves ULiege; Hick, Anne-Christine; Labasse, Alain et al

in Osteoarthritis and Cartilage (2020, April), 28(Suppl 1), 327-28

Detailed reference viewed: 36 (6 ULiège)
Full Text
Peer Reviewed
See detailBoswellia serrata extract and curcumin increase GDF15 production by human primary osteoarthritis chondrocytes: a new mechanism of action
Sanchez, Christelle ULiege; Zappia, Jérémie ULiege; Dierckxsens, Yvan et al

in Osteoarthritis and Cartilage (2020, April), 28(Suppl 1), 120

Detailed reference viewed: 36 (6 ULiège)
Full Text
Peer Reviewed
See detailAn oleuropein-based dietary supplement improves joint functionality in older people with high knee joint pain
Horcajada, M-N; Beaumont, M; Sauvageot, N et al

in Osteoarthritis and Cartilage (2020, April), 28(Suppl 1), 472-3

Detailed reference viewed: 25 (1 ULiège)
Full Text
Peer Reviewed
See detailA multicomponent medication promotes chondrogenesis and reduces MMP-13 in primary articular chondrocytes from knee osteoarthritis patients in vitro
Sanchez, Christelle ULiege; Hemmer, Kathrin; Krommelbein, Natascha et al

in Osteoarthritis and Cartilage (2020, April), 28(Suppl 1), 106-107

Detailed reference viewed: 24 (3 ULiège)
Full Text
Peer Reviewed
See detailThe secretome of skeletal muscle cells: A systematic review
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

in Osteoarthritis and Cartilage Open (2020)

Background Proteomic studies of the secretome of skeletal muscle cells can help us understand the processes that govern the synthesis, systemic interactions and organization of skeletal muscle and ... [more ▼]

Background Proteomic studies of the secretome of skeletal muscle cells can help us understand the processes that govern the synthesis, systemic interactions and organization of skeletal muscle and identify proteins that are involved in muscular adaptations to exercise, ageing and degeneration. In this systematic review, we aimed to summarize recent mass-spectrometry based proteomics discoveries on the secretome of skeletal muscle cells in response to disease, exercise or metabolic stress. Methods A literature search was performed in the Medline/Ovid and Scopus electronic bibliographic databases. Only papers reporting the analysis of the secretome by mass spectrometry were included. Results A total of 19 papers met the inclusion criteria for this systematic review. These papers included comparative analysis of differentially expressed proteins between healthy and unhealthy muscle cells and comparison of the secretome of skeletal muscle cells during myogenesis and after insulin stimulation or exercising. The proteins were separated into several categories and their differential secretion was compared. In total, 654 proteins were listed as being present in the secretome of muscle cells. Among them, 30 proteins were differentially regulated by physical exercise, 130 during myogenesis, 114 by dystrophin deficiency, 26 by muscle atrophy, 27 by insulin stimulation and finally 176 proteins secreted by insulin-resistant muscle cells. Conclusions This systematic review of the secretome of skeletal muscle cell in health and disease provides a comprehensive overview of the most regulated proteins in pathological or physiological conditions. These proteins might be therapeutic targets or biochemical markers of muscle diseases. [less ▲]

Detailed reference viewed: 73 (19 ULiège)
Full Text
Peer Reviewed
See detailCurcuminoids and Boswellia serrata extracts combination decreases tendinopathy symptoms: findings from an open-label post-observational study.
Henrotin, Yves ULiege; Dierckxsens, Yvan; Delisse, Gaëlle et al

in Current medical research and opinion (2020)

Background: To investigate the effects of 1-month treatment in addition to standard care with a food supplement containing both Curcuma longa and Boswellia serrata extracts on tendinopathy symptoms ... [more ▼]

Background: To investigate the effects of 1-month treatment in addition to standard care with a food supplement containing both Curcuma longa and Boswellia serrata extracts on tendinopathy symptoms. Method: This open-label, non-controlled, post-observational study included 670 patients suffering from tendinopathy recruited at different sites by Belgian general practitioners. Patients received a medical prescription for 1-month treatment with two tablets twice a day of a pharmaceutical grade food supplement containing both C. longa and B. serrata extracts. Pain and functional limitation were evaluated using a visual analog scale at the inclusion and 1-month treatment later. Patient satisfaction, concomitant drugs intake and side effects were also recorded. Results: After 1-month treatment, pain and functional limitation were significantly improved whatever the cause of tendinopathy, its localization, and the duration of symptoms. The pain score decreased from 6.16 ± 1.53 to 2.98 ± 1.64 (p < .0001), yielding a drop of 51.6% and the functional limitation score fell after 1-month treatment from 5.96 ± 1.73 to 2.88 ± 1.67 (p < .0001) corresponding to a drop of 51.6%. The percentage of patients taking at least one concomitant treatment at the end of the treatment period had decreased from 81.3% to 61.8% (p < .0001). Only 43 (6.5%) patients reported side effects. No severe adverse effects related to the product were reported. Conclusion: The combination of C. longa and B. serrata extracts improves symptoms in patients suffering of tendinopathy and shows a good safety. Although its effect will have to be confirmed in randomized controlled trials, it can be considered as a helpful support of standard symptomatic treatments for tendinopathies. Highlights Tendinopathy is a common disease representing 30% of all consultations with a general practitioner for musculoskeletal disorders. The combination of Curcuminoids and Boswellia serrata extracts are efficient on tendinopathy symptoms in support of standard symptomatic treatments. The combination of Curcuminoids and B. Serrata extract is safe and can be administrated for at least 1 month in addition of analgesic and non-steroidal anti-inflammatory drugs. [less ▲]

Detailed reference viewed: 44 (2 ULiège)
Full Text
Peer Reviewed
See detailSerum NT/CT SIRT1 ratio reflects early osteoarthritis and chondrosenescence.
Batshon, George; Elayyan, Jinan; Qiq, Omar et al

in Annals of the rheumatic diseases (2020), 79(10), 1370-1380

OBJECTIVE: Previous work has established that the deacetylase sirtuin-1 (SIRT1) is cleaved by cathepsin B in chondrocytes subjected to proinflammatory stress, yielding a stable but inactive N-terminal (NT ... [more ▼]

OBJECTIVE: Previous work has established that the deacetylase sirtuin-1 (SIRT1) is cleaved by cathepsin B in chondrocytes subjected to proinflammatory stress, yielding a stable but inactive N-terminal (NT) polypeptide (75SIRT1) and a C-terminal (CT) fragment. The present work examined if chondrocyte-derived NT-SIRT1 is detected in serum and may serve as an investigative and exploratory biomarker of osteoarthritis (OA). METHODS: We developed a novel ELISA assay to measure the ratio of NT to CT of SIRT1 in the serum of human individuals and mice subjected to post-traumatic OA (PTOA) or age-dependent OA (ADOA). We additionally monitored NT/CT SIRT1 in mice subject to ADOA/PTOA followed by senolytic clearance. Human chondrosenescent and non-senescent chondrocytes were exposed to cytokines and analysed for apoptosis and NT/CT SIRT1 ratio in conditioned medium. RESULTS: Wild-type mice with PTOA or ADOA of moderate severity exhibited increased serum NT/CT SIRT1 ratio. In contrast, this ratio remained low in cartilage-specific Sirt1 knockout mice despite similar or increased PTOA and ADOA severity. Local clearance of senescent chondrocytes from old mice with post-traumatic injury resulted in a lower NT/CT ratio and reduced OA severity. While primary chondrocytes exhibited NT/CT ratio increased in conditioned media after prolonged cytokine stimulation, this increase was not evident in cytokine-stimulated chondrosenescent cells. Finally, serum NT/CT ratio was elevated in humans with early-stage OA. CONCLUSIONS: Increased levels of serum NT/CT SIRT1 ratio correlated with moderate OA in both mice and humans, stemming at least in part from non-senescent chondrocyte apoptosis, possibly a result of prolonged inflammatory insult. [less ▲]

Detailed reference viewed: 34 (0 ULiège)
Full Text
Peer Reviewed
See detailREG-O3 chimeric peptide combining growth hormone and somatostatin sequences improves joint function and prevents cartilage degradation in rat model of traumatic knee osteoarthritis.
Montjean, Rodrick; Escaich, Sonia; Paolini, Raffaello et al

in PLoS ONE (2020), 15(4), 0231240

OBJECTIVE: REG-O3 is a 24-aminoacid chimeric peptide combining a sequence derived from growth hormone (GH) and an analog of somatostatin (SST), molecules displaying cartilage repair and anti-inflammatory ... [more ▼]

OBJECTIVE: REG-O3 is a 24-aminoacid chimeric peptide combining a sequence derived from growth hormone (GH) and an analog of somatostatin (SST), molecules displaying cartilage repair and anti-inflammatory properties, respectively. This study aimed to investigate the disease-modifying osteoarthritis drug (DMOAD) potential of REG-O3 by analyzing its effect on pain, joint function and structure, upon injection into osteoarthritic rat knee joint. DESIGN: Osteoarthritis was induced in the right knee of mature male Lewis rats (n = 12/group) by surgical transection of the anterior cruciate ligament (ACLT) combined with partial medial meniscectomy (pMMx). Treatments were administered intra-articularly from fourteen days after surgery through three consecutive injections one week apart. The effect of REG-O3, solubilized in a liposomal solution and injected at either 5, 25 or 50 μg/50 μL, was compared to liposomal (LIP), dexamethasone and hyaluronic acid (HA) solutions. The study endpoints were the pain/function measured once a week throughout the entire study, and the joint structure evaluated eight weeks after surgery using OARSI score. RESULTS: ACLT/pMMx surgery induced a significant modification of weight bearing in all groups. When compared to liposomal solution, REG-O3 was able to significantly improve weight bearing as efficiently as dexamethasone and HA. REG-O3 (25 μg) was also able to significantly decrease OARSI histological global score as well as degeneration of both cartilage and matrix while the other treatments did not. CONCLUSION: This study provides evidence of a remarkable protecting effect of REG-O3 on pain/knee joint function and cartilage/matrix degradation in ACLT/pMMx model of rat osteoarthritis. REG-O3 thus displays an interesting profile as a DMOAD. [less ▲]

Detailed reference viewed: 43 (2 ULiège)
Full Text
See detailGlucosamine et chondroïtine : à titre préventif pour diminuer les coûts liés à l’arthrose
Henrotin, Yves ULiege

in Les cahiers des compléments alimentaires - l'abrégé (2019)

Detailed reference viewed: 33 (2 ULiège)
Full Text
See detailGlucosamine et chondroitine: un intérêt majeur pour les articulations
Henrotin, Yves ULiege

in Les cahiers des compléments alimentaires - l'intégral (2019)

Detailed reference viewed: 32 (1 ULiège)
Full Text
Peer Reviewed
See detailSyndecan-4 is Increased in Osteoarthritic Knee, but not Hip or Shoulder, Articular Hypertrophic Chondrocytes
Sanchez, Christelle ULiege; Lambert, Cécile ULiege; Dubuc, Jean-Emile et al

Conference (2019, October 07)

Detailed reference viewed: 22 (1 ULiège)
Full Text
Peer Reviewed
See detailDevelopment of a muscle cell culture model from human muscle biopsies
Florin, Antoine ULiege; Lambert, Cécile ULiege; Sanchez, Christelle ULiege et al

Poster (2019, October 02)

We developped a human muscle cell culture model that includes a digestion step with an association of collagenase/dispase/CaCl2 and an anti-CD56 immunomagnetic beads step to eliminate contaminating ... [more ▼]

We developped a human muscle cell culture model that includes a digestion step with an association of collagenase/dispase/CaCl2 and an anti-CD56 immunomagnetic beads step to eliminate contaminating fibroblasts. The proliferation is performed in DMEM supplemented with 10% FBS and 2% UG while the differentiation occurs in DMEM supplemented with 0,5% UG. This model may be used to study the effect of some drugs or to study the differentially secreted proteins during certain pathologies. [less ▲]

Detailed reference viewed: 71 (19 ULiège)